Nutritional, physiological, hematological, and biochemical responses of lambs fed increasing levels of Pornunça silage

ABSTRACT: Pornunça (Manihot sp.) is a potential forage to feed ruminants in drylands worldwide; however, evaluations of animal diets are necessary. This study assessed intake and digestibility of dry matter and nutrients, physiological responses, ingestive behavior, water intake, as well as hematological and biochemical responses of lambs fed diets containing increasing levels of Pornunça silage (PS) replacing Tifton-85 bermudagrass hay (Cynodon spp.). Treatments consisted of 0, 33, 66, and 100 % PS considering the roughage portion of the diet (% dry matter DM). The experimental design was completely randomized with six replicates. Cyanide acid (HCN) levels in fresh Pornunça leaves were 207.7 mg kg1 DM and 76.78 mg kg1 DM in PS. Intake of DM and crude protein (CP), and neutral detergent fiber corrected for ash and protein (NDFap) increased linearly with increasing levels of PS in the diet. Digestibility of DM, NDF, and CP were higher with increasing levels of PS. Idle time decreased linearly, while water balance, water intake via food, and total water in feces increased with PS. Nitrogen balance, physiological, and hematological responses were not influenced by treatments, while the final alanine aminotransferase increased for lambs fed all diets evaluated. The PS up to 100 % of the roughage portion increased DM and nutrient intake and digestibility without altering physiological responses and the hematological and biochemical parameters, resulting in greater water intake via food.

Methane emission, milk yield and behavior of ewes kept on pastures or supplemented with concentrate based on corn grain or whole cottonseed / Emissão de metano, produção de leite e comportamento de ovelhas mantidas em pastagem ou suplementadas com concentrado à base de milho grão ou caroço de algodão

This study aimed to evaluate methane emission, milk yield and behavior of ewes kept exclusively on irrigated pasture of Tifton 85 grass (Cynodon spp.) or supplemented with ground corn or whole cottonseed (WCS) based concentrates. Twelve Lacaune x Santa Ines ewes (43.07±0.83 kg of body weight, 77±24 days after parturition, on average) were distributed in replicated 3x3 Latin square. Treatments consisted of three diets: pasture (no concentrate supplementation); corn (pasture + corn-based supplement); whole cottonseed (pasture + whole cottonseed-based supplement), offering 0.5 kg/ewe/day. The WCS group showed the highest concentrate dry matter intake (DMI) (p=0.049) and crude protein (CP) intake (p=0.001) compared to the others. There was no difference on total DMI (p=0.115) for the tested diets. Animals exclusively kept on pasture had the greatest forage DMI (p=0.004), lowest CP digestibility (p=0.015), showed longer grazing time (p=0.01) and shorter idle time (p=0.01) compared to the supplemented groups. Milk yield (0.36 kg/ewe/day) (p=0.15) and methane emission (33.12 g/ewe/day) (p=0.95) were similar for all three evaluated groups. Supplementation with concentrate based on corn or whole cottonseed does not improve productive performance nor decrease methane emission. However, lactating ewes kept exclusively in pasture show longer grazing time, without changes in milk yield and methane emission.

Objetivou-se avaliar a emissão de metano, a produção de leite e o comportamento de ovelhas mantidas exclusivamente em pastagem irrigada de Tifton 85 (Cynodon spp.) ou suplementadas com concentrados à base de milho grão ou caroço de algodão. Doze ovelhas Lacaune x Santa Inês (43,07±0,83 kg de peso corporal e 77±24 dias após a parição, em média) foram distribuídas em quadrados latinos 3x3 replicados. Os tratamentos consistiram em três dietas: pastagem (sem suplementação concentrada); milho (pastagem + suplemento à base de milho); caroço de algodão integral (pastagem + suplemento à base de caroço de algodão), oferecendo 0,5 kg/ovelha/dia. O grupo caroço de algodão apresentou maior consumo de matéria seca (CMS) (P=0,049) e consumo de proteína bruta (PB) (P=0,001) em relação aos demais. Não houve diferença no CMS total (P=0,115) para as dietas testadas. Os animais exclusivamente em pastagem apresentaram maior consumo de forragem (P=0,004), menor digestibilidade da PB (P=0,015), maior tempo em pastejo (P=0,01) e menor tempo em ócio (P=0,01), em relação aos grupos suplementados. A produção de leite (0,36 kg/ovelha/dia) (P=0,15) e a emissão de metano (33,12 g/ovelha/dia) (P=0,95) foram semelhantes nos três grupos avaliados. A suplementação com concentrado à base de milho grão ou caroço de algodão não melhorou o desempenho produtivo e não reduziu a emissão de metano. Entretanto, as ovelhas lactantes mantidas exclusivamente em pastagem apresentaram maior tempo em pastejo, sem alterações na produção de leite e na emissão de metano.

Nutritional, physiological, hematological, and biochemical responses of lambs fed increasing levels of Pornunça silage

Pornunça (Manihot sp.) is a potential forage to feed ruminants in drylands worldwide; however, evaluations of animal diets are necessary. This study assessed intake and digestibility of dry matter and nutrients, physiological responses, ingestive behavior, water intake, as well as hematological and biochemical responses of lambs fed diets containing increasing levels of Pornunça silage (PS) replacing Tifton-85 bermudagrass hay (Cynodon spp.). Treatments consisted of 0, 33, 66, and 100 % PS considering the roughage portion of the diet (% dry matter - DM). The experimental design was completely randomized with six replicates. Cyanide acid (HCN) levels in fresh Pornunça leaves were 207.7 mg kg-¹ DM and 76.78 mg kg-¹ DM in PS. Intake of DM and crude protein (CP), and neutral detergent fiber corrected for ash and protein (NDFap) increased linearly with increasing levels of PS in the diet. Digestibility of DM, NDF, and CP were higher with increasing levels of PS. Idle time decreased linearly, while water balance, water intake via food, and total water in feces increased with PS. Nitrogen balance, physiological, and hematological responses were not influenced by treatments, while the final alanine aminotransferase increased for lambs fed all diets evaluated. The PS up to 100 % of the roughage portion increased DM and nutrient intake and digestibility without altering physiological responses and the hematological and biochemical parameters, resulting in greater water intake via food.

Weaning and concentrate supplementation on the characteristics of carcass cuts and longissimus muscle of Suffolk lambs finished on pasture

The aim of this study was to evaluate the influence of weaning and concentrate supplementation on the characteristics of carcass cuts and longissimus muscle of Suffolk lambs finished on a Tifton 85 (Cynodon spp.) pasture. Weaning and concentrate supplementation strategies were evaluated in four finishing systems: i) non-weaned and non-supplemented lambs; ii) non-weaned lambs supplemented with concentrate in creep feeding; iii) weaned and non-supplemented lambs; and iv) weaned lambs supplemented with concentrate. In the systems with weaning, lambs were weaned at 46 ± 6 days of age. In the systems with supplementation, the concentrate was offered daily at 2% of lambs' body weight. Weaning led to a decrease, whereas supplementation led to an increase in carcass cut weights. Supplemented lambs had lower yields of neck and uncovered ribs, and higher yields of breast + flank and loin. The characteristics of loin eye and fat thickness over the longissimus muscle presented lower values in weaned lambs and higher values in supplemented lambs. Keeping lambs with their dams and offering concentrate supplementation until slaughter are strategies recommended to obtain heavier cuts with increased muscularity and fat content.(AU)

Plant poisoning containing hydrocyanic acid in cattle in Southern Brazil

Background: Cyanogenic plants accumulate cyanogenic glycosides and release hydrocyanic acid (HCN). In Brazil, therehave been reports of several plants that cause HCN poisoning in animals and lead to a fast death with few clinical signs andlesions on post mortem examination. Some cultivars of Cynodon spp. grasses cause HCN poisoning in cattle in Brazil. Theobjectives of this work were to report the occurrence of deaths by HCN poisoning in cattle as diagnosed by the veterinarypathology laboratory, describe the quantity of HCN in some cultivars of Cynodon spp., as well as, to describe one cultivarof genus Cynodon never reported as poisonous.Materials, Methods & Results: The archives of the Veterinary Pathology Laboratory (LPV) at the Concórdia Campus ofthe Instituto Federal Catarinense (IFC) were reviewed, seeking cases with a diagnosis of hydrocyanic acid poisoning incattle after post mortem examination. The amount of HCN present in some cultivars of the Cynodon genus was quantified due to the high frequency of poisoning cases. From the 1,235 post mortem examinations of cattle 28 (2.27%) werediagnosed with spontaneous hydrocyanic acid poisoning, 17 cases (60.7%) due to ingestion of Prunus sp. or Manihotsp., and 11 cases (39.3%) of Cynodon dactylon ingestion. Most animals were found dead, normally having presented noclinical signs. Macroscopic evaluation mainly showed a severe amount of unchewed and undigested leaves or grass mixedin the ruminal content presenting a bitter almond odor. It was possible to infer that, among cultivars of the Cynodon genus, Florakirk showed the highest levels of HCN compared (P < 0.05) with Star of Puerto Rico, Tifton 68, Tifton 44, andCoast-Cross. Furthermore, Tifton 85 and Jiggs showed undetected levels of HCN...

Plant poisoning containing hydrocyanic acid in cattle in Southern Brazil

Background: Cyanogenic plants accumulate cyanogenic glycosides and release hydrocyanic acid (HCN). In Brazil, therehave been reports of several plants that cause HCN poisoning in animals and lead to a fast death with few clinical signs andlesions on post mortem examination. Some cultivars of Cynodon spp. grasses cause HCN poisoning in cattle in Brazil. Theobjectives of this work were to report the occurrence of deaths by HCN poisoning in cattle as diagnosed by the veterinarypathology laboratory, describe the quantity of HCN in some cultivars of Cynodon spp., as well as, to describe one cultivarof genus Cynodon never reported as poisonous.Materials, Methods & Results: The archives of the Veterinary Pathology Laboratory (LPV) at the Concórdia Campus ofthe Instituto Federal Catarinense (IFC) were reviewed, seeking cases with a diagnosis of hydrocyanic acid poisoning incattle after post mortem examination. The amount of HCN present in some cultivars of the Cynodon genus was quantified due to the high frequency of poisoning cases. From the 1,235 post mortem examinations of cattle 28 (2.27%) werediagnosed with spontaneous hydrocyanic acid poisoning, 17 cases (60.7%) due to ingestion of Prunus sp. or Manihotsp., and 11 cases (39.3%) of Cynodon dactylon ingestion. Most animals were found dead, normally having presented noclinical signs. Macroscopic evaluation mainly showed a severe amount of unchewed and undigested leaves or grass mixedin the ruminal content presenting a bitter almond odor. It was possible to infer that, among cultivars of the Cynodon genus, Florakirk showed the highest levels of HCN compared (P < 0.05) with Star of Puerto Rico, Tifton 68, Tifton 44, andCoast-Cross. Furthermore, Tifton 85 and Jiggs showed undetected levels of HCN...(AU)

Use of near-infrared spectroscopy for prediction of chemical composition of Tifton 85 grass / Uso da espectroscopia de infravermelho próximo para a predição da composição química de capim Tifton 85

The reduction in the quality, consumption, and digestibility of forage can cause a decrease in animal performance, resulting in losses to the rural producer. Thus, it is important to monitor these characteristics in forage plants to devise strategies or practices that optimize production systems. The aim of this study was to develop and validate prediction models using near-infrared spectroscopy (NIRS) to determine the chemical composition of Tifton 85 grass. Samples of green grass, its morphological structures (whole plant, leaf blade, stem + sheath, and senescent material) and hay, totaling 105 samples were used. Conventional chemical analysis was performed to determine the content of oven-dried samples (ODS), mineral matter (MM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), cellulose (CEL), hemicellulose (HEM), and in vitro dry matter digestibility (IVDMD). Subsequently, all the samples were scanned using a Vis-NIR spectrometer to collect spectral data. Principal component analysis (PCA) was applied to the data set, and modified partial least squares was used to correlate reference values to spectral data. The coefficients of determination (R2) were 0.74, 0.85, 0.98, 0.75, 0.85, 0.71, 0.82, 0.77, and 0.93, and the ratio of performance deviations (RPD) obtained were 1.99, 2.71, 6.46, 2.05, 2.58, 3.84, 1.86, 2.35, 2.09, and 3.84 for ODS, MM, CP, NDF, ADF, ADL, CEL, HEM, and IVDMD, respectively. The prediction models obtained, in general, were considered to be of excellent quality, and demonstrated that the determination of the chemical composition of Tifton 85 grass can be performed using NIRS technology, replacing conventional analysis.(AU)

A redução da qualidade, do consumo e da digestibilidade da forragem pode ocasionar a diminuição do desempenho animal, resultando em prejuízos ao produtor rural. Desta forma, é importante monitorar essas características em plantas forrageiras para definir estratégias ou práticas que otimizem os sistemas de produção. Objetivou-se desenvolver e validar modelos de predição pela espectroscopia de infravermelho próximo (NIRS), para determinar a composição química do capim Tifton 85 (Cynodon spp.). Foram utilizadas amostras de capim verde (planta inteira, lâmina foliar, colmo + bainha e material senescente) e de feno, da mesma gramínea, totalizando 105 amostras. As amostras foram submetidas a análise química convencional para determinação dos teores de amostra seca em estufa (ASE), matéria mineral (MM), proteína bruta (PB), fibra em detergente neutro (FDN), fibra em detergente ácido (FDA), lignina em detergente ácido (LDA), celulose (CEL), hemicelulose (HEM) e digestibilidade in vitro da matéria seca (DIVMS). Posteriormente, todas as amostras foram escaneadas em espectrômetro Vis-NIR, para a coleta dos dados espectrais. Aplicouse a análise de componentes principais (PCA) ao conjunto de amostras, e utilizou-se a regressão por mínimos quadrados parciais modificadas para correlacionar valores de referência aos dados espectrais. Os coeficientes de determinação (R2) foram de 0,74; 0,85; 0,98; 0,75; 0,85; 0,71; 0,82, 0,77 e 0,93 e as taxas de desvio de performance (RPD) de 1,99; 2,71; 6,46; 2,05; 2,58; 3,84; 1,86; 2,35; 2,09 e 3,84 para ASE, MM, PB, FDN, FDA, LDA, CEL, HEM e DIVMS respectivamente, na etapa de validação. Os modelos de predição obtidos, em geral, foram considerados de boa qualidade, e demonstraram que a determinação da composição química do Tifton 85 pode ser realizada pela tecnologia NIRS, em substituição à análise convencional.(AU)

Concentrate supplementation on milk yield, methane and CO2 production in crossbred dairy cows grazing in tropical climate regions

The objective of this study was to evaluate the level of concentrate supplementation on the production and chemical composition of milk from 12 crossbred F1 dual-purpose cows (½ Bos taurus ½ Bos indicus) and estimate the emission of CH4, N2O, and CO2 gases. The study included 12 crossbred F1 dual-purpose cows over 60 days of lactation. The cows grazed on 28% tropical native grassland and 72% Brachiaria spp. and Cynodon neumfluensis, supplemented with 0, 150, 300, and 450g of concentrate per kg daily milk production, during three experimental periods of 15 days each in a crossover design. Pasture and concentrate samples were collected and were analyzed for dry matter, crude protein, neutral detergent fiber, and acid detergent fiber. Milk production (kg d-1) was recorded daily, nitrous oxide (N2O), and emissions from excreta and daily CH4 production were calculated. Results were analyzed with the SAS MIXED procedure. Concentrate supplementation in tropical crossbred dairy cows did not improve milk yield but increased CH4 and N2O production (P < 0.0001) per cow as the concentrate increased in the diet; the Ym factor from the tropical region yielded less CH4 than the IPCC Ym model (P < 0.0001). In conclusion, the calculation of CH4 using specific emission factors for the tropical climate region is better than the IPCC default emission factors in order not to overestimate the CH4 emissions.(AU)

Concentrate supplementation on milk yield, methane and CO2 production in crossbred dairy cows grazing in tropical climate regions

The objective of this study was to evaluate the level of concentrate supplementation on the production and chemical composition of milk from 12 crossbred F1 dual-purpose cows (½ Bos taurus ½ Bos indicus) and estimate the emission of CH4, N2O, and CO2 gases. The study included 12 crossbred F1 dual-purpose cows over 60 days of lactation. The cows grazed on 28% tropical native grassland and 72% Brachiaria spp. and Cynodon neumfluensis, supplemented with 0, 150, 300, and 450g of concentrate per kg daily milk production, during three experimental periods of 15 days each in a crossover design. Pasture and concentrate samples were collected and were analyzed for dry matter, crude protein, neutral detergent fiber, and acid detergent fiber. Milk production (kg d-1) was recorded daily, nitrous oxide (N2O), and emissions from excreta and daily CH4 production were calculated. Results were analyzed with the SAS MIXED procedure. Concentrate supplementation in tropical crossbred dairy cows did not improve milk yield but increased CH4 and N2O production (P < 0.0001) per cow as the concentrate increased in the diet; the Ym factor from the tropical region yielded less CH4 than the IPCC Ym model (P < 0.0001). In conclusion, the calculation of CH4 using specific emission factors for the tropical climate region is better than the IPCC default emission factors in order not to overestimate the CH4 emissions.(AU)

Concentrate supplementation on milk yield, methane and CO2 production in crossbred dairy cows grazing in tropical climate regions

The objective of this study was to evaluate the level of concentrate supplementation on the production and chemical composition of milk from 12 crossbred F1 dual-purpose cows (½ Bos taurus – ½ Bos indicus) and estimate the emission of CH4, N2O, and CO2 gases. The study included 12 crossbred F1 dual-purpose cows over 60 days of lactation. The cows grazed on 28% tropical native grassland and 72% Brachiaria spp. and Cynodon neumfluensis, supplemented with 0, 150, 300, and 450g of concentrate per kg daily milk production, during three experimental periods of 15 days each in a crossover design. Pasture and concentrate samples were collected and were analyzed for dry matter, crude protein, neutral detergent fiber, and acid detergent fiber. Milk production (kg d-1) was recorded daily, nitrous oxide (N2O), and emissions from excreta and daily CH4 production were calculated. Results were analyzed with the SAS MIXED procedure. Concentrate supplementation in tropical crossbred dairy cows did not improve milk yield but increased CH4 and N2O production (P < 0.0001) per cow as the concentrate increased in the diet; the Ym factor from the tropical region yielded less CH4 than the IPCC Ym model (P < 0.0001). In conclusion, the calculation of CH4 using specific emission factors for the tropical climate region is better than the IPCC default emission factors in order not to overestimate the CH4 emissions.

Nutrient digestibility in horses of tropical grasses found in semi-arid areas of the Brazilian Northeast region assessed using mobile bags

The present study used mobile bags to estimate horse nutrient digestibility of tropical grasses found in semi-arid areas of the Brazilian Northeast region. Five female mixed-breed horses with a mean weight of 400±23 kg were assigned to a 5×5 Latin square design with five periods of seven days and five grasses: Tifton 85 hay (Cynodon spp.), sixweeks threeawn (Aristida adscensionis Linn.), Alexandergrass (Brachiaria plantaginea (Link) Hitchc), capim-de-raiz (Chloris orthonoton Doell), and Sabi grass (Urochloa mosambicensis). The nutrient content of forages was determined prior to inoculation in horses and after recovery of mobile bags from feces. The digestibility coefficients were determined from the difference between the inoculated and recovered material. The dry matter, organic matter, mineral matter, crude protein, neutral detergent fiber, and acid detergent fiber contents of the grass species were analyzed. Digestibility data were subjected to analysis of variance using the Statistical Analysis System (SAS, version 9.0) software. Higher dry matter digestibility coefficients were observed in Tifton 85 (74.61%), Alexandergrass (74.30%), and capim-de-raiz (68.88%) than in sixweeks threeawn (48.40%) and Sabi grass (52.89%). The highest crude protein digestibility coefficients were found for Alexandergrass (95.70%), Tifton 85 (93.50%), and sixweeks threeawn (93.35%). Sixweeks threeawn had lower apparent mineral matter digestibility than the other grasses. The digestibility coefficients of Alexandergrass and capim-de-raiz indicate that those grasses have potential to be used in equine feed.

Changes in soil chemical and physical properties in pasture fertilised with liquid swine manure

Chemical and physical properties of soil can be altered with the successive application of liquid swine manure (LSM), also known as slurry. Therefore, monitoring the impact of LSM is essential to an assessment of the potential agronomic and environmental benefits and risks associated with management practices. The aim of this study was to evaluate the effects of LSM on the chemical and physical attributes of soil under pasture (Cynodon spp.), located in southern Brazil. Four areas were sampled: three areas cultivated with Cynodon spp. pasture with successive applications of 200 m3 ha1 y1 of LSM for three, eight and 15 years; and untreated native forest as a reference. The soil attributes evaluated were: organic carbon (OC), active (pH water) and potential acidity (H + Al+ 3), nutrient availability (i.e., P, K, Ca, Mg, Cu and Zn), soil bulk density, macro, micro and total porosity. Successive applications of LSM on pasture reduced soil active acidity, and increased the soil organic C and plant-available N, P, Ca, Mg, Cu and Zn contents. These effects were more intense with time and in the shallowest layers, i.e. 0-10 cm. Excessive increases in P, Cu and Zn in the soil should be carefully monitored to minimize the contamination risks to soil, ground and surface waters. Soil physical attribute changes were more associated with land use (pasture versus native forest) than LSM use, and LSM applications induced slight improvements in the soil structural quality over time.

Changes in soil chemical and physical properties in pasture fertilised with liquid swine manure

Chemical and physical properties of soil can be altered with the successive application of liquid swine manure (LSM), also known as slurry. Therefore, monitoring the impact of LSM is essential to an assessment of the potential agronomic and environmental benefits and risks associated with management practices. The aim of this study was to evaluate the effects of LSM on the chemical and physical attributes of soil under pasture (Cynodon spp.), located in southern Brazil. Four areas were sampled: three areas cultivated with Cynodon spp. pasture with successive applications of 200 m3 ha1 y1 of LSM for three, eight and 15 years; and untreated native forest as a reference. The soil attributes evaluated were: organic carbon (OC), active (pH water) and potential acidity (H + Al+ 3), nutrient availability (i.e., P, K, Ca, Mg, Cu and Zn), soil bulk density, macro, micro and total porosity. Successive applications of LSM on pasture reduced soil active acidity, and increased the soil organic C and plant-available N, P, Ca, Mg, Cu and Zn contents. These effects were more intense with time and in the shallowest layers, i.e. 0-10 cm. Excessive increases in P, Cu and Zn in the soil should be carefully monitored to minimize the contamination risks to soil, ground and surface waters. Soil physical attribute changes were more associated with land use (pasture versus native forest) than LSM use, and LSM applications induced slight improvements in the soil structural quality over time.(AU)

Canopy structure and morphogenesis of Italian ryegrass intercropped with red clover under cutting intervals determined by thermal sum / Estrutura do dossel e morfogênese do azevém anual consorciado com trevo vermelho em intervalos de corte determinados por soma térmica

The canopy structure and morphogenesis of ryegrass (Lolium multiflorum Lam.) was investigated after it was over seeded with Tifton 85 (Cynodon spp.) and intercropped with red clover (Trifolium pratense L.). Time intervals between cuts were determined by different thermal sums (125, 250, 375, and 500 degree-days [DD]). Cuts were conducted at a height equivalent to 50% of the average canopy height. Morphogenic and structural data were collected from July to October of 2016 and 2017, from a completely randomized block experimental design. Canopy height, extended tiller height, pseudostem length, and forage mass were fitted to linear equations according to the thermal sums. Interaction of ryegrass with red clover was the highest (12.1%) for longer cut intervals (375 and 500 DD). The leaf appearance rate (0.0080 leaf/DD), phyllochron value (128.8 DD), and senescence rate (0.10 cm/DD) of ryegrass were comparable between the different intervals. The slowest elongation rate (0.06 cm/DD) with the shortest interval duration between cuts (171.6 DD) was observed in the 125 DD interval. According to the findings from this 2-year experiment, the use of a 250 DD cut interval was recommended between grazing events, taking into account the efficiency of forage use, ryegrass leaf, stem ratio, and interactions with red clover in the canopy under rotational stocking.(AU)

A estrutura do dossel e a morfogênese do azevém (Lolium multiflorum Lam.) foram investigadas após a sobressemeadura em Tifton 85 (Cynodon spp.) e consorciado com trevo vermelho (Trifolium pratense L.). Os intervalos de tempo entre os cortes foram determinados por diferentes somas térmicas (125, 250, 375 e 500 graus-dia [GD]). Os cortes foram realizados a uma altura equivalente a 50% da altura média do dossel. Os dados morfogênicos e estruturais foram coletados no período de julho a outubro de 2016 e 2017, em um delineamento experimental de blocos ao acaso. A altura do dossel, a altura estendida do perfilho, o comprimento do pseudocolmo e a massa de forragem se ajustaram a equações lineares de acordo com a soma térmica. A consorciação de azevém e trevo vermelho foi maior (12,1%) para intervalos de corte mais longos (375 e 500 GD). A taxa de aparecimento foliar (0,0080 folha/GD), filocrono (128,8 GD) e a taxa de senescência (0,10 cm /GD) do azevém não foram diferentes entre os intervalos de corte. A menor taxa de elongação (0,06 cm/GD) e duração de elongação (171,6 GD) foi observada no menor intervalo entre cortes, 125 GD. De acordo com os achados deste experimento de dois anos, recomenda-se o uso de um intervalo de corte de 250 GD entre os eventos de pastejo, levando em consideração a eficiência do uso de forragem, proporção de folhas e colmo de azevém e trevo vermelho no dossel, sob lotação rotativa.(AU)

Protein and carbohydrate fractionation of Jiggs Bermudagrass in different seasons and under intermittent grazing by Holstein cows

Jiggs bermudagrass has presented competitive production potential over other forages. However, there is a lack of information about the nutritional value of this forage with important relevance in ruminant nutrition. This study aimed to evaluate the protein and carbohydrate fractionation of Jiggs bermudagrass in different seasons of the year under intermittent grazing by Holstein cows. The experiment was conducted during one year in a completely randomized design with nine replications and treatments consisted of the effect of four seasons: fall, winter, spring and summer. The results showed that there were seasonal variations in the fractions of proteins and carbohydrate, with the exception of the protein fraction B3. The better climatic conditions in spring and summer contributed to an increase in the protein fraction A and carbohydrate A+B1. The winter had a greater fraction C of protein and carbohydrate, reflecting the nutritive value of the forage. The use of irrigation during fall and winter had a positive effect on nitrogen fractions B1 and B2 and on non-fiber carbohydrates.(AU)

Protein and carbohydrate fractionation of Jiggs Bermudagrass in different seasons and under intermittent grazing by Holstein cows

Jiggs bermudagrass has presented competitive production potential over other forages. However, there is a lack of information about the nutritional value of this forage with important relevance in ruminant nutrition. This study aimed to evaluate the protein and carbohydrate fractionation of Jiggs bermudagrass in different seasons of the year under intermittent grazing by Holstein cows. The experiment was conducted during one year in a completely randomized design with nine replications and treatments consisted of the effect of four seasons: fall, winter, spring and summer. The results showed that there were seasonal variations in the fractions of proteins and carbohydrate, with the exception of the protein fraction B3. The better climatic conditions in spring and summer contributed to an increase in the protein fraction A and carbohydrate A+B1. The winter had a greater fraction C of protein and carbohydrate, reflecting the nutritive value of the forage. The use of irrigation during fall and winter had a positive effect on nitrogen fractions B1 and B2 and on non-fiber carbohydrates.

Clinical biochemistry profile of American Quarter Horse broodmares fed Tifton-85 (Cynodon spp. ) hay and haylage / Perfil bioquímico clínico de matrizes Quarto de Milha alimentadas com feno e haylage Tifton 85 (Cynodon spp. )

The conservation of haylage (a pre-dried feed) can be challenging, since there is an increased risk of mould growth, which can contaminate this foodstuff with mycotoxins. However, when the hygienic quality is secured, haylage enhances grass palatability and provide enough supply of dry matter throughout the year. Due to the lack of information regarding its effect on blood parameters in horses fed exclusively with this foodstuff, the aim of this study is to provide information regarding its use in comparison to hay and ensure that it does not affect horses' biochemical profile. Twelve Quarter Horse broodmares were distributed into two groups, each fed with Tifton-85 (Cynodon spp.) hay or haylage for a period of 28 days, and the biochemical profile was done in five different times (T0 before the experiment started and, chronologically, seven days apart - T1, T2, T3 and T4), It was analyzed total protein (TP) and its fractioning; enzymes alanine aminotransferase, aspartate aminotransferase and γ-glutamyl-transferase; endogenous catabolism products urea and creatinine; and ions calcium and phosphorus. Mycotoxins in haylage were also investigated and remained below the legislation thresholds. Only TP was higher in the last sampling (T4) of the haylage group, which may be related to the foodstuff's higher protein digestibility. No differences were observed between serum enzymes, urea, creatinine and Ca/P from both experimental groups. Haylage has proven to be safe, when well prepared for horses, without causing impairing side effects, as shown by the normal serum biochemistry parameters presented in this study.(AU)

A conservação do haylage (alimento pré-seco) pode ser desafiadora, considerando o aumento do risco de crescimento de fungos, com consequente produção de micotoxinas. Entretanto, quando a qualidade da higiene e armazenamento é assegurada, o haylage aumenta a palatabilidade da forragem e fornece suplemento de matéria seca suficiente ao longo do ano. Devido à falta de informação relativa aos efeitos dessa alimentação nos parâmetros sanguíneos de equinos alimentados exclusivamente com essa dieta, o objetivo do presente estudo é avaliar o perfil bioquímico sanguíneo dos equinos após administração da haylage em comparação com feno. Doze matrizes Quarto de Milha foram distribuídas em dois grupos, cada um recebendo feno ou haylage de Tifton 85 (Cynodon spp.) por um período de 28 dias. O perfil bioquímico foi realizado em cinco tempos (T) diferentes (T0, antes do início do experimento e cronologicamente, a cada sete dias após o fornecimento das dietas - T1, T2, T3 e T4) para análise de proteína total (PT) e seu perfil fracionado, das enzimas alanina aminotransferase, aspartato aminotransferase, γ-glutamil-transferase, dos produtos de catabolismo creatinina e ureia e, dos íons cálcio e fósforo. Micotoxinas no haylage foram investigadas e mantiveram-se abaixo dos limites determinados pela legislação brasileira. O perfil bioquímico revelou, somente, elevação da PT em T4 no grupo que recebeu haylage, o que pode estar relacionado à sua maior digestibilidade proteica. Nenhuma diferença foi observada nos outros parâmetros estudados em ambos os grupos experimentais. Conclui-se que Haylage é comprovadamente seguro, quando bem preparado para equinos, sem causar efeitos na saúde geral, conforme demonstrado pelos exames bioquímicos no presente estudo.(AU)

Clinical biochemistry profile of American Quarter Horse broodmares fed Tifton-85 (Cynodon spp. ) hay and haylage / Perfil bioquímico clínico de matrizes Quarto de Milha alimentadas com feno e haylage Tifton 85 (Cynodon spp. )

The conservation of haylage (a pre-dried feed) can be challenging, since there is an increased risk of mould growth, which can contaminate this foodstuff with mycotoxins. However, when the hygienic quality is secured, haylage enhances grass palatability and provide enough supply of dry matter throughout the year. Due to the lack of information regarding its effect on blood parameters in horses fed exclusively with this foodstuff, the aim of this study is to provide information regarding its use in comparison to hay and ensure that it does not affect horses' biochemical profile. Twelve Quarter Horse broodmares were distributed into two groups, each fed with Tifton-85 (Cynodon spp.) hay or haylage for a period of 28 days, and the biochemical profile was done in five different times (T0 before the experiment started and, chronologically, seven days apart - T1, T2, T3 and T4), It was analyzed total protein (TP) and its fractioning; enzymes alanine aminotransferase, aspartate aminotransferase and γ-glutamyl-transferase; endogenous catabolism products urea and creatinine; and ions calcium and phosphorus. Mycotoxins in haylage were also investigated and remained below the legislation thresholds. Only TP was higher in the last sampling (T4) of the haylage group, which may be related to the foodstuff's higher protein digestibility. No differences were observed between serum enzymes, urea, creatinine and Ca/P from both experimental groups. Haylage has proven to be safe, when well prepared for horses, without causing impairing side effects, as shown by the normal serum biochemistry parameters presented in this study.(AU)

A conservação do haylage (alimento pré-seco) pode ser desafiadora, considerando o aumento do risco de crescimento de fungos, com consequente produção de micotoxinas. Entretanto, quando a qualidade da higiene e armazenamento é assegurada, o haylage aumenta a palatabilidade da forragem e fornece suplemento de matéria seca suficiente ao longo do ano. Devido à falta de informação relativa aos efeitos dessa alimentação nos parâmetros sanguíneos de equinos alimentados exclusivamente com essa dieta, o objetivo do presente estudo é avaliar o perfil bioquímico sanguíneo dos equinos após administração da haylage em comparação com feno. Doze matrizes Quarto de Milha foram distribuídas em dois grupos, cada um recebendo feno ou haylage de Tifton 85 (Cynodon spp.) por um período de 28 dias. O perfil bioquímico foi realizado em cinco tempos (T) diferentes (T0, antes do início do experimento e cronologicamente, a cada sete dias após o fornecimento das dietas - T1, T2, T3 e T4) para análise de proteína total (PT) e seu perfil fracionado, das enzimas alanina aminotransferase, aspartato aminotransferase, γ-glutamil-transferase, dos produtos de catabolismo creatinina e ureia e, dos íons cálcio e fósforo. Micotoxinas no haylage foram investigadas e mantiveram-se abaixo dos limites determinados pela legislação brasileira. O perfil bioquímico revelou, somente, elevação da PT em T4 no grupo que recebeu haylage, o que pode estar relacionado à sua maior digestibilidade proteica. Nenhuma diferença foi observada nos outros parâmetros estudados em ambos os grupos experimentais. Conclui-se que Haylage é comprovadamente seguro, quando bem preparado para equinos, sem causar efeitos na saúde geral, conforme demonstrado pelos exames bioquímicos no presente estudo.(AU)

Bermuda grass foliar chlorophyll content influenced by luminosity and substrate / Conteúdo de clorofila foliar influenciado pela luminosidade e substrato em grama Bermuda

Bermuda grass (Cynodon spp.) is the best suited for ornamental and sportive lawns, widely used in fields of soccer, polo, golf, tennis, baseball and lining among ornamental components of a garden. Regardless of use, a very important characteristic for a good quality lawn is the intensity of green coloration that it presents. The objective of this work was to evaluate the influence of luminosity and different substrates on Bermuda grass (Cynodondactylonx C. transvaalensis) chlorophyll a and b contents. The experimental design was completely randomized in a 5 x 4 factorial scheme (substrates x luminosities), in completely randomized design with 20 treatments and 3 replicates. The substrates were: S1 = Soil, S2 = Soil + sand (2:1), S3 = Soil + organic matter (1:1), S4 = Soil + organic matter + sand (2:1:1) and S5 = organic matter + sand (3:1), in four luminosity conditions: full sun, 30%, 50% and 80% of shading for six months. We evaluated chlorophyll a and b. After 30 DAP the levels of chlorophyll content decreased in relation to shading increased the opposite was found at 180 days after grass planting, higher levels of chlorophyll content were observed as shading increased. The substrate formulations used have not interfered at leaves chlorophyll a and b content.

A grama Bermuda (Cynodon spp.) é uma das espécies de forração mais utilizadas em campos de futebol, polo, tenis, beisebol, além de fazer parte de components ornamentais em jardins. Além de qualquer uso, uma característica muito importante de qualquer gramado é a colocação verde que possui. Objetivou-se com o experimento avaliar a influência da luminosidade e de diferentes substratos no teor de clorofila das folhas e avaliação visual do sistema radicular de grama bermuda (Cynodon dactylonx C. transvaalensis). O delineamento experimental foi inteiramente casualizado em esquema fatorial 5 x 4 (substratos x luminosidades), em delineamento inteiramente casualizado com 20 tratamentos e 3 repetições. Os substratos foram: S1 = Solo, S2 = Solo + areia (2:1), S3 = Solo + matéria orgânica (1:1), S4 = Solo + matéria orgânica + areia (2:1:1) e S5 = Matéria orgânica + areia (3:1), em quatro condições de luminosidade: pleno sol, 30%, 50% e 80% de sombreamento, durante seis meses. Foram avaliados: clorofila a e b. Após 30 dias os níveis de conteúdo de clorofila diminuíram conforme aumentou o sombreamento e o oposto foi encontrado aos 180 dias do plantio da grama, em que maiores teores de clorofila ocorreu com aumento do sombreamento.

Bermuda grass foliar chlorophyll content influenced by luminosity and substrate / Conteúdo de clorofila foliar influenciado pela luminosidade e substrato em grama Bermuda

Bermuda grass (Cynodon spp.) is the best suited for ornamental and sportive lawns, widely used in fields of soccer, polo, golf, tennis, baseball and lining among ornamental components of a garden. Regardless of use, a very important characteristic for a good quality lawn is the intensity of green coloration that it presents. The objective of this work was to evaluate the influence of luminosity and different substrates on Bermuda grass (Cynodondactylonx C. transvaalensis) chlorophyll a and b contents. The experimental design was completely randomized in a 5 x 4 factorial scheme (substrates x luminosities), in completely randomized design with 20 treatments and 3 replicates. The substrates were: S1 = Soil, S2 = Soil + sand (2:1), S3 = Soil + organic matter (1:1), S4 = Soil + organic matter + sand (2:1:1) and S5 = organic matter + sand (3:1), in four luminosity conditions: full sun, 30%, 50% and 80% of shading for six months. We evaluated chlorophyll a and b. After 30 DAP the levels of chlorophyll content decreased in relation to shading increased the opposite was found at 180 days after grass planting, higher levels of chlorophyll content were observed as shading increased. The substrate formulations used have not interfered at leaves chlorophyll a and b content.(AU)

A grama Bermuda (Cynodon spp.) é uma das espécies de forração mais utilizadas em campos de futebol, polo, tenis, beisebol, além de fazer parte de components ornamentais em jardins. Além de qualquer uso, uma característica muito importante de qualquer gramado é a colocação verde que possui. Objetivou-se com o experimento avaliar a influência da luminosidade e de diferentes substratos no teor de clorofila das folhas e avaliação visual do sistema radicular de grama bermuda (Cynodon dactylonx C. transvaalensis). O delineamento experimental foi inteiramente casualizado em esquema fatorial 5 x 4 (substratos x luminosidades), em delineamento inteiramente casualizado com 20 tratamentos e 3 repetições. Os substratos foram: S1 = Solo, S2 = Solo + areia (2:1), S3 = Solo + matéria orgânica (1:1), S4 = Solo + matéria orgânica + areia (2:1:1) e S5 = Matéria orgânica + areia (3:1), em quatro condições de luminosidade: pleno sol, 30%, 50% e 80% de sombreamento, durante seis meses. Foram avaliados: clorofila a e b. Após 30 dias os níveis de conteúdo de clorofila diminuíram conforme aumentou o sombreamento e o oposto foi encontrado aos 180 dias do plantio da grama, em que maiores teores de clorofila ocorreu com aumento do sombreamento.(AU)