Rhinitis in goat by Exserohilum rostratum (Setosphaeria rostrata) / Rinite em caprino por Exserohilum rostratum (Setosphaeria rostrata)

ABSTRACT: We described a case of rhinitis case caused by Exserohilum rostratum in a 3-year-old, mixed breed, afemale goat, presenting with decreased appetite, dyspnea, and face deformity, with evolution of approximately 2 months. Necropsy revealed an extensive brownish yellow, irregular, ulcerated, and friable focal lesion in the nasal cavity. Purulent secretion drained from the lesion, affecting the nasal vestibule and extending the caudal portion of the dorsal concha, associated with nasal meatus obstruction, destruction of the nasal septum, and nasal bone palatine and vomer. Histologically, the lesion was characterized as rhinitis and pyogranulomatous and necrotizing osteomyelitis (multifocal to coalescent, sharp, and chronic) and associated with a myriad of fungal structures. Morphologically, the hyphae were characterized by thin, slightly tortuous, and rarely septate walls (2-6 μm diameter), multiple chains of individual conidia or in groups (8-10 μm diameter), and pigmented chlamydoconidia (7.5-15 μm diameter). They showed positive results for the special histochemical techniques such as Fontana Masson, methenamine silver nitrate by Grocott, and periodic acid by Schiff. E. rostratum was identified as the causative agent using microbiological isolation associated with molecular identification. Pyrogranulomatous rhinitis caused by E. rostratum in goats is unprecedented, being the first isolation of the agent in animals in Brazil and the first case of nasal phaeohyphomycosis in the goats.

RESUMO: Descreve-se um caso de rinite por Exserohilum rostratum em um caprino, sem raça definida, fêmea, três anos, com diminuição do apetite, dispneia e deformidade facial, com evolução de aproximadamente dois meses. Durante a necropsia, observou-se que na cavidade nasal havia área focal extensa, amarelo acastanhada, irregular, ulcerada e friável, que drenava secreção purulenta acometendo o vestíbulo nasal e estendendo-se da porção caudal da concha dorsal, associada a obstrução do meato nasal, destruição do septo nasal e osso nasal palatino e vômer. Histologicamente, a lesão foi caracterizada como rinite e osteomielite piogranulomatosa e necrosante, multifocal a coalescente, aguda, crônica, associada a uma miríade de estruturas fúngicas. Morfologicamente, as hifas foram caracterizadas por paredes finas, discretamente tortuosas e septadas medindo 2 a 6 μm de diâmetro. Observamos algumas cadeias de conídios ou grupos com 8 a 10 μm de diâmetro e clamidoconídios pigmentados medindo de 7,5 a 15 μm de diâmetro. Foram positivos na histoquímica especial de Fontana Masson, Nitrato de Metenamina de Prata de Grocott e Ácido Periódico de Schiff. O agente foi determinado por meio de isolamento microbiológico associado à identificação molecular. A rinite piogranulomatosa causada por E. rostratum em caprinos é inédita, sendo o primeiro isolamento do agente em animais no Brasil e o primeiro caso de feo-hifomicose nasal na espécie caprina.

Rhinitis in goat by Exserohilum rostratum (Setosphaeria rostrata) / Rinite em caprino por Exserohilum rostratum (Setosphaeria rostrata)

We described a case of rhinitis case caused by Exserohilum rostratum in a 3-year-old, mixed breed, afemale goat, presenting with decreased appetite, dyspnea, and face deformity, with evolution of approximately 2 months. Necropsy revealed an extensive brownish yellow, irregular, ulcerated, and friable focal lesion in the nasal cavity. Purulent secretion drained from the lesion, affecting the nasal vestibule and extending the caudal portion of the dorsal concha, associated with nasal meatus obstruction, destruction of the nasal septum, and nasal bone palatine and vomer. Histologically, the lesion was characterized as rhinitis and pyogranulomatous and necrotizing osteomyelitis (multifocal to coalescent, sharp, and chronic) and associated with a myriad of fungal structures. Morphologically, the hyphae were characterized by thin, slightly tortuous, and rarely septate walls (2-6 µm diameter), multiple chains of individual conidia or in groups (8-10 µm diameter), and pigmented chlamydoconidia (7.5-15 µm diameter). They showed positive results for the special histochemical techniques such as Fontana Masson, methenamine silver nitrate by Grocott, and periodic acid by Schiff. E. rostratum was identified as the causative agent using microbiological isolation associated with molecular identification. Pyrogranulomatous rhinitis caused by E. rostratum in goats is unprecedented, being the first isolation of the agent in animals in Brazil and the first case of nasal phaeohyphomycosis in the goats.

Descreve-se um caso de rinite por Exserohilum rostratum em um caprino, sem raça definida, fêmea, três anos, com diminuição do apetite, dispneia e deformidade facial, com evolução de aproximadamente dois meses. Durante a necropsia, observou-se que na cavidade nasal havia área focal extensa, amarelo acastanhada, irregular, ulcerada e friável, que drenava secreção purulenta acometendo o vestíbulo nasal e estendendo-se da porção caudal da concha dorsal, associada a obstrução do meato nasal, destruição do septo nasal e osso nasal palatino e vômer. Histologicamente, a lesão foi caracterizada como rinite e osteomielite piogranulomatosa e necrosante, multifocal a coalescente, aguda, crônica, associada a uma miríade de estruturas fúngicas. Morfologicamente, as hifas foram caracterizadas por paredes finas, discretamente tortuosas e septadas medindo 2 a 6 µm de diâmetro. Observamos algumas cadeias de conídios ou grupos com 8 a 10 µm de diâmetro e clamidoconídios pigmentados medindo de 7,5 a 15 µm de diâmetro. Foram positivos na histoquímica especial de Fontana Masson, Nitrato de Metenamina de Prata de Grocott e Ácido Periódico de Schiff. O agente foi determinado por meio de isolamento microbiológico associado à identificação molecular. A rinite piogranulomatosa causada por E. rostratum em caprinos é inédita, sendo o primeiro isolamento do agente em animais no Brasil e o primeiro caso de feo-hifomicose nasal na espécie caprina.

Systemic phaeohyphomycosis in a dog caused by Cladophialophora bantiana

Background: Cladophialophora bantiana is a dematiaceous fungus that causes phaeohyphomycosis, a generic term used to describe a variety of unusual mycoses caused by fungi that have melanin in their cell wall. C. bantiana targets the central nervous system, commonly causing localized brain infections that may result in disseminated infections. In Brazil, minimal phaeohyphomycosis data are available, and information about C. bantiana infections in animals, especially canines, is scarce. Thus, the aim of this study was to describe the clinical and pathological aspects of systemic phaeohyphomycosis caused by C. bantiana in a dog. Case: A 1-year-old female Pit Bull presented with weight loss, reduced appetite, and a history of cutaneous lesions on the right thoracic limb; however, clinical evolution was not reported. The bitch had reportedly given birth recently. Physical examination revealed thinness, pale ocular and oral mucosa, submandibular lymph nodes, and enlarged popliteal lymph nodes. The bitch died after convulsive crises during hospitalization. At necropsy, white-yellowish multifocal nodules were observed in the liver and right kidney. The brain featured left cerebral hemisphere asymmetry with blood vessel congestion in the leptomeninges and an irregular brownish focal area on the surface of the right occipital cortex. Cross-sections of the formalin-fi xed brain exhibited compression of the left lateral ventricle and the presence of grayish and friable multifocal areas in the gray matter of the left parietal and right occipital cortices. Fragments of the lesions were collected for histopathological and microbiological examination. Histologically, the lesions were similar, characterized by hepatitis, nephritis, and granulomatous and necrotizing...

Antifungal activity of geraniol and citronellol against food-relevant dematiaceous fungi Cladosporium spp / Atividade antifúngica de geraniol e citronelol frente a fungos dematiáceos de importância para os alimentos do gênero Cladosporium spp

Cladosporium spp. is a group of dematiaceous food-relevant fungi which are well dispersed in the environment causing food spoilage and poisoning. Considering the importance of fungal contamination, natural drugs to control their growth have become important. Thus, the aim of this study was to evaluate the inhibitory effects of two monoterpenoids, (geraniol and citronellol), against strains of Cladosporium carrioni, C. cladosporioides,and C. oxysporum. Methods: The Minimum Inhibitory Concentration (MIC) and Minimum Fungicide Concentration (MFC) of the drugs were determined by microdilution. The effects of test drugs on mycelial dry weight, conidia germination, and conidiogenesis of Cladosporium spp. were also investigated using a hemacytometer. Respective MIC and MFC values of citronellol varied from 256 to 512 μg/mL, and from 256 to 2048 μg/mL. The MIC and MFC of geraniol varied similarly to citronellol. Conidia germination, mycelial dry weight, and conidiogenesis of Cladosporium spp. were reduced by the test-drugs at 1/2MIC, MIC and 2xMIC (p<0.05). These measurable cell events are essential for fungal infection and development in foods. The action of citronellol and geraniol against Cladosporium spp. suggest that the drugs may serve as effective agents for controlling fungal contamination and growth in foods.

Cladosporium spp. é um grupo de fungos dematiáceos relevantes para os alimentos, que podem ser dispersos pelo ambiente e causar deterioração e intoxicação alimentar. Considerando a importância da contaminação fúngica, os produtos naturais usados para controlar seu crescimento são importantes. neste contexto, o objetivo deste estudo foi avaliar os efeitos inibitórios de dois monoterpenoides, geraniol e citronelol, contra cepas de Cladosporium carrioni, C. cladosporioides e C. oxysporum. A Concentração Inibitória Mínima (CIM) e Concentração Fungicida Mínima (CFM) das drogas foram determinadas por microdiluição. os efeitos das drogas-teste sobre a massa micelial seca, a germinação de conídios e a conidiogênese de Cladosporium spp. também foram investigados utilizando um hemocitômetro. os valores de CIM e CFM do citronelol variaram de 256 a 512 μg/mL e de 256 a 2048 μg/mL, respectivamente. CIM e CFM de geraniol variaram de forma semelhante. A germinação de conídios, massa micelial seca e conidiogênese de Cladosporium spp. foram inibidas pelas drogas-teste 1/2CIM, CIM e 2xCIM (p<0,05). Esses eventos celulares são essenciais para a infecção e desenvolvimento fúngico em alimentos. A ação de citronelol e geraniol contra Cladosporium spp. sugere que podem servir como agentes eficazes para controlar a contaminação fúngica e seu crescimento em alimentos.

Antifungal activity of geraniol and citronellol against food-relevant dematiaceous fungi Cladosporium spp / Atividade antifúngica de geraniol e citronelol frente a fungos dematiáceos de importância para os alimentos do gênero Cladosporium spp

Cladosporium spp. is a group of dematiaceous food-relevant fungi which are well dispersed in the environment causing food spoilage and poisoning. Considering the importance of fungal contamination, natural drugs to control their growth have become important. Thus, the aim of this study was to evaluate the inhibitory effects of two monoterpenoids, (geraniol and citronellol), against strains of Cladosporium carrioni, C. cladosporioides,and C. oxysporum. Methods: The Minimum Inhibitory Concentration (MIC) and Minimum Fungicide Concentration (MFC) of the drugs were determined by microdilution. The effects of test drugs on mycelial dry weight, conidia germination, and conidiogenesis of Cladosporium spp. were also investigated using a hemacytometer. Respective MIC and MFC values of citronellol varied from 256 to 512 μg/mL, and from 256 to 2048 μg/mL. The MIC and MFC of geraniol varied similarly to citronellol. Conidia germination, mycelial dry weight, and conidiogenesis of Cladosporium spp. were reduced by the test-drugs at 1/2MIC, MIC and 2xMIC (p<0.05). These measurable cell events are essential for fungal infection and development in foods. The action of citronellol and geraniol against Cladosporium spp. suggest that the drugs may serve as effective agents for controlling fungal contamination and growth in foods.(AU)

Cladosporium spp. é um grupo de fungos dematiáceos relevantes para os alimentos, que podem ser dispersos pelo ambiente e causar deterioração e intoxicação alimentar. Considerando a importância da contaminação fúngica, os produtos naturais usados para controlar seu crescimento são importantes. neste contexto, o objetivo deste estudo foi avaliar os efeitos inibitórios de dois monoterpenoides, geraniol e citronelol, contra cepas de Cladosporium carrioni, C. cladosporioides e C. oxysporum. A Concentração Inibitória Mínima (CIM) e Concentração Fungicida Mínima (CFM) das drogas foram determinadas por microdiluição. os efeitos das drogas-teste sobre a massa micelial seca, a germinação de conídios e a conidiogênese de Cladosporium spp. também foram investigados utilizando um hemocitômetro. os valores de CIM e CFM do citronelol variaram de 256 a 512 μg/mL e de 256 a 2048 μg/mL, respectivamente. CIM e CFM de geraniol variaram de forma semelhante. A germinação de conídios, massa micelial seca e conidiogênese de Cladosporium spp. foram inibidas pelas drogas-teste 1/2CIM, CIM e 2xCIM (p<0,05). Esses eventos celulares são essenciais para a infecção e desenvolvimento fúngico em alimentos. A ação de citronelol e geraniol contra Cladosporium spp. sugere que podem servir como agentes eficazes para controlar a contaminação fúngica e seu crescimento em alimentos.(AU)

Isolation of dermatophytes and saprotrophic fungi from the hair coat of cats without skin disorders in the metropolitan area of Porto Alegre - RS, Brazil / Isolamento de dermatófitos e fungos saprotróficos do pelame de gatos sem dermatoses na região metropolitana de Porto Alegre - RS, Brasil

Background: Dermatophytoses are one of the most frequent skin diseases of pets and livestock in the world. Contagionamong animal communities, difficulty in implementing control measures, and the eventual transmission of ringworm topeople explain its great importance. Microsporum canis causes a zoonosis that has increased in several countries, outnumbering classical anthropophilic dermatophytes. The objective of this study was to isolate dermatophytes from the hair coatof cats without skin disorders in the metropolitan area of Porto Alegre, south of Brazil, as well as evaluate the influenceof age, sex, hair length, habitat and access or not to the street as potential risk factors.Materials, Methods & Results: Samples were obtained from 191 cats with no skin disorders by rubbing sterilized carpetsquares on the cats skin (head, neck, dorsum, limbs and tail) and cultured for dermatophytes on Sabouraud dextrose agarwith chloramphenicol and cyclohexamide and incubated at 27°C for up to 21 days. Only the genus Microsporum (8,4%)was isolated from positive specimens: M. canis (5,8%) and M. gypseum (2,6%). On 15 samples (7,8%) there was no fungalgrowth. From the remaining 160 samples (83,8%), several saprotrophic fungi were isolated: hyaline filamentous fungi[Penicillium sp. (27), Aspergillus sp. (23), Scopulariopsis sp. (22), Acremonium sp. (10), Chrysosporium sp. (7), Paecilomyces sp. (7), Fusarium sp. (2) and unidentified hyalohyphomycetes (13)]; dematiaceous filamentous fungi [Cladosporiumsp. (39), Alternaria sp. (10), Curvularia sp. (10) and unidentified phaeohyphomycetes (14)]; Zygomycetes [Rhizopus sp.(2) and Mucor sp. (1)] and yeasts [Malassezia sp. (2) and Candida sp. (4)]. Inittialy, the possibility of association between predictors variables and a variable answer was evaluated by an univariate logistic regression model. Variables with...(AU)

Isolation of dermatophytes and saprotrophic fungi from the hair coat of cats without skin disorders in the metropolitan area of Porto Alegre - RS, Brazil / Isolamento de dermatófitos e fungos saprotróficos do pelame de gatos sem dermatoses na região metropolitana de Porto Alegre - RS, Brasil

Background: Dermatophytoses are one of the most frequent skin diseases of pets and livestock in the world. Contagionamong animal communities, difficulty in implementing control measures, and the eventual transmission of ringworm topeople explain its great importance. Microsporum canis causes a zoonosis that has increased in several countries, outnumbering classical anthropophilic dermatophytes. The objective of this study was to isolate dermatophytes from the hair coatof cats without skin disorders in the metropolitan area of Porto Alegre, south of Brazil, as well as evaluate the influenceof age, sex, hair length, habitat and access or not to the street as potential risk factors.Materials, Methods & Results: Samples were obtained from 191 cats with no skin disorders by rubbing sterilized carpetsquares on the cats’ skin (head, neck, dorsum, limbs and tail) and cultured for dermatophytes on Sabouraud dextrose agarwith chloramphenicol and cyclohexamide and incubated at 27°C for up to 21 days. Only the genus Microsporum (8,4%)was isolated from positive specimens: M. canis (5,8%) and M. gypseum (2,6%). On 15 samples (7,8%) there was no fungalgrowth. From the remaining 160 samples (83,8%), several saprotrophic fungi were isolated: hyaline filamentous fungi[Penicillium sp. (27), Aspergillus sp. (23), Scopulariopsis sp. (22), Acremonium sp. (10), Chrysosporium sp. (7), Paecilomyces sp. (7), Fusarium sp. (2) and unidentified hyalohyphomycetes (13)]; dematiaceous filamentous fungi [Cladosporiumsp. (39), Alternaria sp. (10), Curvularia sp. (10) and unidentified phaeohyphomycetes (14)]; Zygomycetes [Rhizopus sp.(2) and Mucor sp. (1)] and yeasts [Malassezia sp. (2) and Candida sp. (4)]. Inittialy, the possibility of association between predictors variables and a variable answer was evaluated by an univariate logistic regression model. Variables with...

Feo-hifomicose sistêmica em cão

The objective of this study was to describe clinical, laboratorial and histopathological findings of systemic phaeohyphomycosis in a dog. The animal was presented with a history of fatigue, ascites, lethargy, weight and appetite loss and polydipsia. The therapy for erliquiose and babesiosis was started. After 11 days, the dog returned with severe liver disease and died after forty-eight hours. At necropsy, there were black nodules in various abdominal organs. Histologically, necrotizing granulomatous reaction with hemorrhage associated with myriads of intralesional pigmented fungal hyphae was observed in multiple organs, characterizing systemic phaeohyphomycosis. Thus, it is important to include this illness in the differential diagnosis of severe liver diseases in dogs with ascites.

Descrevem-se os achados clínicos, laboratoriais e histopatológicos de feo-hifomicose sistêmica em um cão. Clinicamente observou-se cansaço, ascite, apatia, hiporexia, perda de peso e polidipsia. O animal estava sendo tratado para erliquiose e babesiose. Após 11 dias, o cão retornou com hepatopatia grave e veio a óbito em 48 horas. Na necropsia, observaram-se nódulos enegrecidos em vários órgãos da cavidade abdominal. Histologicamente, havia reação granulomatosa necrosante com hemorragia, associada a miríades de hifas fúngicas pigmentadas intralesionais em múltiplos órgãos, caracterizando feo-hifomicose sistêmica. Ressalta-se a importância de incluir esta doença no diagnóstico diferencial de hepatopatias graves em cães com ascite.

Feo-hifomicose sistêmica em cão

The objective of this study was to describe clinical, laboratorial and histopathological findings of systemic phaeohyphomycosis in a dog. The animal was presented with a history of fatigue, ascites, lethargy, weight and appetite loss and polydipsia. The therapy for erliquiose and babesiosis was started. After 11 days, the dog returned with severe liver disease and died after forty-eight hours. At necropsy, there were black nodules in various abdominal organs. Histologically, necrotizing granulomatous reaction with hemorrhage associated with myriads of intralesional pigmented fungal hyphae was observed in multiple organs, characterizing systemic phaeohyphomycosis. Thus, it is important to include this illness in the differential diagnosis of severe liver diseases in dogs with ascites.

Descrevem-se os achados clínicos, laboratoriais e histopatológicos de feo-hifomicose sistêmica em um cão. Clinicamente observou-se cansaço, ascite, apatia, hiporexia, perda de peso e polidipsia. O animal estava sendo tratado para erliquiose e babesiose. Após 11 dias, o cão retornou com hepatopatia grave e veio a óbito em 48 horas. Na necropsia, observaram-se nódulos enegrecidos em vários órgãos da cavidade abdominal. Histologicamente, havia reação granulomatosa necrosante com hemorragia, associada a miríades de hifas fúngicas pigmentadas intralesionais em múltiplos órgãos, caracterizando feo-hifomicose sistêmica. Ressalta-se a importância de incluir esta doença no diagnóstico diferencial de hepatopatias graves em cães com ascite.

Feohifomicose cutânea causada por Curvularia sp. em um equino / Cutaneous phaeohyphomycosis caused by Curvularia sp. in an equine

As feohifomicoses são doenças cutâneas, subcutâneas e sistêmicas que acometem humanos e animais. São causadas por fungos que possuem melanina em sua parede celular (dematiáceos) e estão geralmente associadas com imunocomprometimento do hospedeiro. Este trabalho descreve alterações clínicas e histopatológicas de feohifomicose cutânea em um equino, macho, da raça crioula, de 6 anos de idade. O animal apresentou dois nódulos cutâneos com aproximadamente 4 a 5 cm de diâmetro, nas regiões laterais direita e esquerda do abdômen, que foram removidos cirurgicamente e encaminhados para exame histopatológico. Macroscopicamente, as lesões consistiam em nódulos cutâneos, um ulcerado, apresentando ao corte múltiplas áreas circunscritas e esbranquiçadas contendo em seu interior grânulos enegrecidos. Na histopatologia, as lesões se caracterizavam por dermatite ulcerativa piogranulomatosa com hifas septadas intralesionais apresentando parede pigmentada. No cultivo micológico, observou-se crescimento fúngico de Curvularia sp. O diagnóstico de dermatite fúngica por Curvularia sp. foi baseado nos achados histológicos associados com o isolamento fúngico. O tratamento se restringiu à retirada cirúrgica completa das lesões; entretanto, um ano após a excisão, novas lesões surgiram nas proximidades das lesões prévias no abdômen.

Phaeohyphomycosis include cutaneous, subcutaneous, and systemic diseases that affect humans and animals. The diseases are usually associated with immunocompromised hosts and are caused by fungi whose wall cell contains melanin (dematiaceous). This communication describes clinical, histopathological, and microbiological aspects observed in a case of phaeohyphomycosis affecting a 6-year-old male Crioulo horse. Animal showed two cutaneous nodules with about 4-5 cm of diameter located in the lateral abdominal walls (one in the right, the other in the left). Nodules were excised and submitted to the pathological laboratory SPV-UFRGS. Grossly, one of the cutaneous nodules was ulcerated and had, at the cut surface, multiple circumscript whitish areas containing blackish granules. Microscopically, nodules had piogranulomatous ulcerative dermatitis associated with intralesional septate and pigmented hyphae. Mycological culture, performed at the Mycology Laboratory-UFRGS, yielded fungal growth of Curvularia sp. Those findings support the diagnosis of fungal dermatitis by Curvularia sp. Treatment was complete surgical excision of lesions; however, one year after the procedure, recurrence of lesions was observed in the areas close to that where the previous lesions were.

Feohifomicose cutânea causada por Curvularia sp. em um equino / Cutaneous phaeohyphomycosis caused by Curvularia sp. in an equine

As feohifomicoses são doenças cutâneas, subcutâneas e sistêmicas que acometem humanos e animais. São causadas por fungos que possuem melanina em sua parede celular (dematiáceos) e estão geralmente associadas com imunocomprometimento do hospedeiro. Este trabalho descreve alterações clínicas e histopatológicas de feohifomicose cutânea em um equino, macho, da raça crioula, de 6 anos de idade. O animal apresentou dois nódulos cutâneos com aproximadamente 4 a 5 cm de diâmetro, nas regiões laterais direita e esquerda do abdômen, que foram removidos cirurgicamente e encaminhados para exame histopatológico. Macroscopicamente, as lesões consistiam em nódulos cutâneos, um ulcerado, apresentando ao corte múltiplas áreas circunscritas e esbranquiçadas contendo em seu interior grânulos enegrecidos. Na histopatologia, as lesões se caracterizavam por dermatite ulcerativa piogranulomatosa com hifas septadas intralesionais apresentando parede pigmentada. No cultivo micológico, observou-se crescimento fúngico de Curvularia sp. O diagnóstico de dermatite fúngica por Curvularia sp. foi baseado nos achados histológicos associados com o isolamento fúngico. O tratamento se restringiu à retirada cirúrgica completa das lesões; entretanto, um ano após a excisão, novas lesões surgiram nas proximidades das lesões prévias no abdômen.(AU)

Phaeohyphomycosis include cutaneous, subcutaneous, and systemic diseases that affect humans and animals. The diseases are usually associated with immunocompromised hosts and are caused by fungi whose wall cell contains melanin (dematiaceous). This communication describes clinical, histopathological, and microbiological aspects observed in a case of phaeohyphomycosis affecting a 6-year-old male Crioulo horse. Animal showed two cutaneous nodules with about 4-5 cm of diameter located in the lateral abdominal walls (one in the right, the other in the left). Nodules were excised and submitted to the pathological laboratory SPV-UFRGS. Grossly, one of the cutaneous nodules was ulcerated and had, at the cut surface, multiple circumscript whitish areas containing blackish granules. Microscopically, nodules had piogranulomatous ulcerative dermatitis associated with intralesional septate and pigmented hyphae. Mycological culture, performed at the Mycology Laboratory-UFRGS, yielded fungal growth of Curvularia sp. Those findings support the diagnosis of fungal dermatitis by Curvularia sp. Treatment was complete surgical excision of lesions; however, one year after the procedure, recurrence of lesions was observed in the areas close to that where the previous lesions were.(AU)

Feohifomicose felina por Phialophera verrucosa - Primeiro relato de caso em Curitiba-PR, Brasil / Feline phaeohyphomycosis due to Phialophera verrucosa - First case report in Curitiba - PR, Brasil

Fungos oportunistas podem eventualmente acometer os felinos, causando micoses profundas. A feohifomicose é causada por espécies fi lamentosas dematiáceas, inoculadas acidentalmente através de traumas, mordidas ou arranhões.A doença pode manifestar-se nas formas nasal, rino-sinusal, cutânea, subcutânea, sistêmica e cerebral. O diagnóstico pode ser subsidiado pelos exames citológico ehistopatológico, mas somente através da cultura o agente etiológico pode ser identifi cado. Apresentase um caso de feohifomicose em um felino doméstico adulto onde Phialophora verrucosa foi identifi cada, na região de Curitiba, PR, Brasil. (AU)

Opportunistic fungi may eventually affect the cats, causing deep mycoses. Phaeohyphomycosis is caused by dematiaceous fi lamentous species, accidentally inoculated by trauma, bites or scratches. The disease can manifest itself in ways nose, rhino-sinus, skin, subcutaneous, systemic and cerebral.Diagnosis can be subsidized by cytological and histopathological examinations but only by culturingthe causative agent can be identifi ed. We present a case of phaeohyphomycosis in an adult domesticcat where Phialophora verrucosa was identifi ed, in the region of Curitiba, PR, Brazil. (AU)

Feohifomicose felina por Phialophera verrucosa - Primeiro relato de caso em Curitiba-PR, Brasil / Feline phaeohyphomycosis due to Phialophera verrucosa - First case report in Curitiba - PR, Brasil

Fungos oportunistas podem eventualmente acometer os felinos, causando micoses profundas. A feohifomicose é causada por espécies fi lamentosas dematiáceas, inoculadas acidentalmente através de traumas, mordidas ou arranhões.A doença pode manifestar-se nas formas nasal, rino-sinusal, cutânea, subcutânea, sistêmica e cerebral. O diagnóstico pode ser subsidiado pelos exames citológico ehistopatológico, mas somente através da cultura o agente etiológico pode ser identifi cado. Apresentase um caso de feohifomicose em um felino doméstico adulto onde Phialophora verrucosa foi identifi cada, na região de Curitiba, PR, Brasil.

Opportunistic fungi may eventually affect the cats, causing deep mycoses. Phaeohyphomycosis is caused by dematiaceous fi lamentous species, accidentally inoculated by trauma, bites or scratches. The disease can manifest itself in ways nose, rhino-sinus, skin, subcutaneous, systemic and cerebral.Diagnosis can be subsidized by cytological and histopathological examinations but only by culturingthe causative agent can be identifi ed. We present a case of phaeohyphomycosis in an adult domesticcat where Phialophora verrucosa was identifi ed, in the region of Curitiba, PR, Brazil.

Occurrence of fungi of superficial lesion of dog in the city of São Paulo - Brazil / Ocorrência de fungos em lesões superficiais de cães na cidade de São Paulo, Brasil

It is presented the result of a mycological study performed in 212 dogs which showed superficial tesions clinically related to dermatomycosis, during the period of January till December, 1986. Through the direct microscopic examination and cultures isolation from the specimens, there were identified the following etiologic agents: Malassezia pachydermatis (49,5%); Microsporum canis (30,0%); M. gypseum (4,7%); Trichophyton mentagrophytes (0,9%). Several different genera were identified in low frequencies as follows: Candida; Trichosporon; Torulopsis; Geotrichum; Cephalosporium; ScopuI ariopsis and a dematiaceous fungi. The highest frequency of dermatomycosis occurred in the group of dogs which had less than 11 months old.

Foram estudados 212 casos de cães com suspeita clinica de dermatomicose, no período de janeiro a dezembro de 1986. Deste total investigado, foram diagnosticados, através de exame microscópico direto e cultivo, 107 casos de dermatomicoses (50,5%). Malassezia pachydermatis foi o agente predominante (43,5%) seguido dos dermatófitos Microsporum canis (30,0%), M. Gypseum (4,7%), Trichophyton mentagrophytes (0,9%). Outros fungos como Candida sp, Trichosporon sp, Torulopsis sp, Geotrichum sp, Cephalosporium sp, Scopularopsis sp e fungos dematáceos foram isolados, sendo discutível sua participação na etiologia primária das lesões. De acordo com a faixa etária, a maior prevalência ocorreu no grupo de até 11 meses e com referência ao sexo, não houve predominância significativa.