Danger under wheels: mammal roadkills in the threaten lowland Atlantic Forest in southeast Brazil / Perigo sob rodas: atropelamentos de mamíferos na ameaçada Mata Atlântica de baixada no sudeste do Brasil

Understanding the ecological impacts of roads on mammals requires periodic monitoring of roads, and identification of both temporal and spatial distribution of roadkills (i.e., roadkill hotspots). The main aim of the study was to identify the most roadkilled mammals and evaluate the temporal and spatial distribution of roadkills in the RJ-122, a highway that crosses the threaten lowland Atlantic Forest in the state of Rio de Janeiro, southeast Brazil. Between October 2017 and January 2020, an intense monitoring study was conducted, with the carcasses of the roadkilled mammals being collected three times a week. Overall, we recorded 295 roadkilled mammals belonging to 22 species, resulting in 11 roadkills per month for the RJ-122 highway. The black-eared opossum, Didelphis aurita (N=149, 51%) stood out as the most roadkilled mammal, followed by porcupine, Coendou spinosus (N= 24, 8%), crab-eating fox, Cerdocyon thous (N= 23, 8), nine-banded armadillo, Dasypus novemcinctus (N= 23, 8%), and white-tufted marmoset, Callithrix jacchus (N= 20, 7%). Roadkills on the RJ-122 varied throughout the year, being more frequent in the rainy season (N=180) than in the dry season (N=115), and were concentrated in two hotspots, indicating some critical points with high roadkill frequency. Hotspots were associated with areas with dense natural vegetation, which can function as forest corridors in this fragmented landscape. Based on our results, several mitigation measures are recommended for the RJ-122 highway.(AU)

Compreender os impactos ecológicos das estradas sobre a fauna de mamíferos requer o monitoramento periódico das estradas e a identificação da distribuição temporal e espacial dos atropelamentos (ou seja, hotspots de atropelamentos). O objetivo principal do estudo foi identificar os mamíferos mais atropelados e avaliar a distribuição temporal e espacial dos atropelamentos na RJ-122, uma rodovia que atravessa a ameaçada Mata Atlântica de baixada no estado do Rio de Janeiro, sudeste do Brasil. Entre outubro de 2017 e janeiro de 2020, foi realizado um intenso estudo de monitoramento, com a coleta três vezes por semana das carcaças dos mamíferos atropelados. Ao todo, registramos 295 mamíferos atropelados pertencentes a 22 espécies, resultando em 11 atropelamentos por mês para a rodovia RJ-122. O gambá-de-orelha-preta, Didelphis aurita (N=149, 51%) destacou-se como o mamífero mais atropelado, seguido pelo ouriço-cacheiro, Coendou spinosus (N= 24, 8%), cachorro-do-mato, Cerdocyon thous (N= 23, 8%), tatu-galinha, Dasypus novemcinctus (N= 23, 8%) e sagui-de-tufo-branco, Callithrix jacchus (N= 20, 7%). Os atropelamentos na RJ-122 variaram ao longo do ano, sendo mais frequentes na estação chuvosa (N=180) do que na estação seca (N=115), e se concentraram em duas áreas, indicando alguns pontos críticos com alta frequência de atropelamentos. Os hotspots foram associados a áreas com vegetação natural densa, que podem funcionar como corredores florestais nesta paisagem fragmentada. Com base em nossos resultados, várias medidas de mitigação são recomendadas para a rodovia RJ-122.(AU)

How omnivory affects the survival and choices of earwig Doru luteipes (Scudder) (Dermaptera: Forficulidae)?

Abstract Doru luteipes (Scudder, 1876) is an omnivorous predator that finds different food resources in the corn plant: eggs of Spodoptera frugiperda (J.E. Smith, 1797), uredospores of Puccinia polysora (Underw, 1897), and pollen. Knowing the survival and food preferences of this predator is essential to define its relevance as a biological control agent. We hypothesize that the foraging behavior and predatory capacity of D. luteipes may be affected when several food resources, especially eggs of S. frugiperda, uredospores of P. polysora, and pollen are concurrently in the same plant. The survival of D. luteipes in the nymph stage and their preference among food resources, often available in corn plants, were determined. To verify the survival of D. luteipes, newly hatched nymphs were fed exclusively with 1- uredospores of P. polysora, 2- eggs of S. frugiperda, 3- corn pollen, 4- a combination of uredospores + eggs, and 5- artificial diet (control). In another experiment, nymphs and adults of D. luteipes with 24 and 48 hours of fasting were individually released in the center of a container with four diets: 1- uredospores of P. polysora, 2- eggs of S. frugiperda, 3- corn pollen, 4- artificial diet, and maintained for 10 minutes, to evaluate the food choice and feeding time. The exclusive feeding with S. frugiperda eggs caused low nymph survival (8%), but the combination of P. polysora uredospores + S. frugiperda eggs allowed 58.3% survival. D. luteipes preferred feeding during the nighttime and the most significant proportions of choices by nymphs and adults were for pollen and diet, with adults spending more time eating pollen. These findings indicate that the trophic choices of D. luteipes are relevant to understand its contribution as an agent to control pest insects and fungal diseases in corn.

Resumo Doru luteipes (Scudder, 1876) é um predador onívoro, que encontra na planta do milho diferentes recursos alimentares: ovos de Spodoptera frugiperda (J.E. Smith, 1797), uredósporos de Puccinia polysora (Underw, 1897) e pólen. Para definição da relevância desse predador como agente de controle biológico, conhecer a sobrevivência e preferência alimentar é essencial. Nós hipotetizamos que o comportamento de forrageamento e a capacidade de predação de D. luteipes podem ser afetados quando uma mesma planta oferece ovos, uredósporos e pólen, concomitantemente. A sobrevivência de D. luteipes na fase de ninfa e sua preferência entre os recursos alimentares, frequentemente disponíveis nas plantas de milho, foram determinados. Para verificar a sobrevivência de D. luteipes, ninfas recém eclodidas foram alimentadas exclusivamente com 1- uredósporos de P. polysora, 2- ovos de S. frugiperda, 3- pólen de milho, 4- combinação de uredósporos + ovos e 5- dieta artificial (controle). Em outro experimento, ninfas e adultos de D. luteipes com 24 e 48 horas de jejum foram liberadas individualmente, em recipientes contendo quatro dietas: 1- uredósporos de P. polysora, 2- ovos de S. frugiperda, 3- pólen de milho, 4- dieta artificial e mantidas durante 10 min, sendo avaliados a escolha pelo alimento e o tempo de alimentação. A alimentação exclusiva com ovos de S. frugiperda ocasionou baixa sobrevivência das ninfas (8%), porém a combinação de uredósporos de P. polysora + ovos de S. frugiperda possibilitou sobrevivência de 58,3%. D. luteipes preferiu se alimentar durante o período noturno e as maiores proporções de escolhas das ninfas e dos adultos ocorreram no pólen e na dieta, sendo que os adultos gastaram mais tempo se alimentando de pólen. Estas descobertas indicam que as escolhas tróficas de D. luteipes são relevantes para compreender sua contribuição como agente de controle de insetos-praga e doença fúngicas em milho.

Ingestive behavior of Holstein × Zebu cows managed on deferred supplemented pasture

This study aimed to evaluate the ingestive behavior of F1 Holstein × Zebu cows grazing on deferred pasture receiving different supplementation strategies. The experimental design was completely randomized, in a 3×4 factorial, with three supplementation strategies and four evaluation periods. The ingestive behavior of 24 cows divided into three groups of supplementation provided at milking was observed, and two of these groups received protein in the pasture. Ingestive behavior was monitored every 10 minutes for 24 hours and analyzed in the form of periods (morning, afternoon, night, and dawn) and total time. The animals remained longer grazing during the afternoon when the BGHI presented values of 83.8. Protein consumption did not influence grazing time but interfered with forage consumption. For the total grazing time, the animals in group 2 spent less time grazing. The groups of animals presented no difference concerning the time spent on rumination and idleness. During night and dawn, when the climate was characterized as thermal comfort, the animals spent more time ruminating. The climatic conditions did not influence the ingestive behavior, as the grazing peak was observed at times of higher BGHI. However, supplementation of the animals in group 2 modified the ingestive behavior of F1 H × Z cows.

How omnivory affects the survival and choices of earwig Doru luteipes (Scudder) (Dermaptera: Forficulidae)? / Como a onivoria afeta a sobrevivência e escolhas da tesourinha Doru luteipes (Scudder) (Dermaptera: Forficulidae)?

Abstract Doru luteipes (Scudder, 1876) is an omnivorous predator that finds different food resources in the corn plant: eggs of Spodoptera frugiperda (J.E. Smith, 1797), uredospores of Puccinia polysora (Underw, 1897), and pollen. Knowing the survival and food preferences of this predator is essential to define its relevance as a biological control agent. We hypothesize that the foraging behavior and predatory capacity of D. luteipes may be affected when several food resources, especially eggs of S. frugiperda, uredospores of P. polysora, and pollen are concurrently in the same plant. The survival of D. luteipes in the nymph stage and their preference among food resources, often available in corn plants, were determined. To verify the survival of D. luteipes, newly hatched nymphs were fed exclusively with 1- uredospores of P. polysora, 2- eggs of S. frugiperda, 3- corn pollen, 4- a combination of uredospores + eggs, and 5- artificial diet (control). In another experiment, nymphs and adults of D. luteipes with 24 and 48 hours of fasting were individually released in the center of a container with four diets: 1- uredospores of P. polysora, 2- eggs of S. frugiperda, 3- corn pollen, 4- artificial diet, and maintained for 10 minutes, to evaluate the food choice and feeding time. The exclusive feeding with S. frugiperda eggs caused low nymph survival (8%), but the combination of P. polysora uredospores + S. frugiperda eggs allowed 58.3% survival. D. luteipes preferred feeding during the nighttime and the most significant proportions of choices by nymphs and adults were for pollen and diet, with adults spending more time eating pollen. These findings indicate that the trophic choices of D. luteipes are relevant to understand its contribution as an agent to control pest insects and fungal diseases in corn.

Resumo Doru luteipes (Scudder, 1876) é um predador onívoro, que encontra na planta do milho diferentes recursos alimentares: ovos de Spodoptera frugiperda (J.E. Smith, 1797), uredósporos de Puccinia polysora (Underw, 1897) e pólen. Para definição da relevância desse predador como agente de controle biológico, conhecer a sobrevivência e preferência alimentar é essencial. Nós hipotetizamos que o comportamento de forrageamento e a capacidade de predação de D. luteipes podem ser afetados quando uma mesma planta oferece ovos, uredósporos e pólen, concomitantemente. A sobrevivência de D. luteipes na fase de ninfa e sua preferência entre os recursos alimentares, frequentemente disponíveis nas plantas de milho, foram determinados. Para verificar a sobrevivência de D. luteipes, ninfas recém eclodidas foram alimentadas exclusivamente com 1- uredósporos de P. polysora, 2- ovos de S. frugiperda, 3- pólen de milho, 4- combinação de uredósporos + ovos e 5- dieta artificial (controle). Em outro experimento, ninfas e adultos de D. luteipes com 24 e 48 horas de jejum foram liberadas individualmente, em recipientes contendo quatro dietas: 1- uredósporos de P. polysora, 2- ovos de S. frugiperda, 3- pólen de milho, 4- dieta artificial e mantidas durante 10 min, sendo avaliados a escolha pelo alimento e o tempo de alimentação. A alimentação exclusiva com ovos de S. frugiperda ocasionou baixa sobrevivência das ninfas (8%), porém a combinação de uredósporos de P. polysora + ovos de S. frugiperda possibilitou sobrevivência de 58,3%. D. luteipes preferiu se alimentar durante o período noturno e as maiores proporções de escolhas das ninfas e dos adultos ocorreram no pólen e na dieta, sendo que os adultos gastaram mais tempo se alimentando de pólen. Estas descobertas indicam que as escolhas tróficas de D. luteipes são relevantes para compreender sua contribuição como agente de controle de insetos-praga e doença fúngicas em milho.

How omnivory affects the survival and choices of earwig Doru luteipes (Scudder) (Dermaptera: Forficulidae)? / Como a onivoria afeta a sobrevivência e escolhas da tesourinha Doru luteipes (Scudder) (Dermaptera: Forficulidae)?

Doru luteipes (Scudder, 1876) is an omnivorous predator that finds different food resources in the corn plant: eggs of Spodoptera frugiperda (J.E. Smith, 1797), uredospores of Puccinia polysora (Underw, 1897), and pollen. Knowing the survival and food preferences of this predator is essential to define its relevance as a biological control agent. We hypothesize that the foraging behavior and predatory capacity of D. luteipes may be affected when several food resources, especially eggs of S. frugiperda, uredospores of P. polysora, and pollen are concurrently in the same plant. The survival of D. luteipes in the nymph stage and their preference among food resources, often available in corn plants, were determined. To verify the survival of D. luteipes, newly hatched nymphs were fed exclusively with 1- uredospores of P. polysora, 2- eggs of S. frugiperda, 3- corn pollen, 4- a combination of uredospores + eggs, and 5- artificial diet (control). In another experiment, nymphs and adults of D. luteipes with 24 and 48 hours of fasting were individually released in the center of a container with four diets: 1- uredospores of P. polysora, 2- eggs of S. frugiperda, 3- corn pollen, 4- artificial diet, and maintained for 10 minutes, to evaluate the food choice and feeding time. The exclusive feeding with S. frugiperda eggs caused low nymph survival (8%), but the combination of P. polysora uredospores + S. frugiperda eggs allowed 58.3% survival. D. luteipes preferred feeding during the nighttime and the most significant proportions of choices by nymphs and adults were for pollen and diet, with adults spending more time eating pollen. These findings indicate that the trophic choices of D. luteipes are relevant to understand its contribution as an agent to control pest insects and fungal diseases in corn.

Doru luteipes (Scudder, 1876) é um predador onívoro, que encontra na planta do milho diferentes recursos alimentares: ovos de Spodoptera frugiperda (J.E. Smith, 1797), uredósporos de Puccinia polysora (Underw, 1897) e pólen. Para definição da relevância desse predador como agente de controle biológico, conhecer a sobrevivência e preferência alimentar é essencial. Nós hipotetizamos que o comportamento de forrageamento e a capacidade de predação de D. luteipes podem ser afetados quando uma mesma planta oferece ovos, uredósporos e pólen, concomitantemente. A sobrevivência de D. luteipes na fase de ninfa e sua preferência entre os recursos alimentares, frequentemente disponíveis nas plantas de milho, foram determinados. Para verificar a sobrevivência de D. luteipes, ninfas recém eclodidas foram alimentadas exclusivamente com 1- uredósporos de P. polysora, 2- ovos de S. frugiperda, 3- pólen de milho, 4- combinação de uredósporos + ovos e 5- dieta artificial (controle). Em outro experimento, ninfas e adultos de D. luteipes com 24 e 48 horas de jejum foram liberadas individualmente, em recipientes contendo quatro dietas: 1- uredósporos de P. polysora, 2- ovos de S. frugiperda, 3- pólen de milho, 4- dieta artificial e mantidas durante 10 min, sendo avaliados a escolha pelo alimento e o tempo de alimentação. A alimentação exclusiva com ovos de S. frugiperda ocasionou baixa sobrevivência das ninfas (8%), porém a combinação de uredósporos de P. polysora + ovos de S. frugiperda possibilitou sobrevivência de 58,3%. D. luteipes preferiu se alimentar durante o período noturno e as maiores proporções de escolhas das ninfas e dos adultos ocorreram no pólen e na dieta, sendo que os adultos gastaram mais tempo se alimentando de pólen. Estas descobertas indicam que as escolhas tróficas de D. luteipes são relevantes para compreender sua contribuição como agente de controle de insetos-praga e doença fúngicas em milho.

How omnivory affects the survival and choices of earwig Doru luteipes (Scudder) (Dermaptera: Forficulidae)? / Como a onivoria afeta a sobrevivência e escolhas da tesourinha Doru luteipes (Scudder) (Dermaptera: Forficulidae)?

Doru luteipes (Scudder, 1876) is an omnivorous predator that finds different food resources in the corn plant: eggs of Spodoptera frugiperda (J.E. Smith, 1797), uredospores of Puccinia polysora (Underw, 1897), and pollen. Knowing the survival and food preferences of this predator is essential to define its relevance as a biological control agent. We hypothesize that the foraging behavior and predatory capacity of D. luteipes may be affected when several food resources, especially eggs of S. frugiperda, uredospores of P. polysora, and pollen are concurrently in the same plant. The survival of D. luteipes in the nymph stage and their preference among food resources, often available in corn plants, were determined. To verify the survival of D. luteipes, newly hatched nymphs were fed exclusively with 1- uredospores of P. polysora, 2- eggs of S. frugiperda, 3- corn pollen, 4- a combination of uredospores + eggs, and 5- artificial diet (control). In another experiment, nymphs and adults of D. luteipes with 24 and 48 hours of fasting were individually released in the center of a container with four diets: 1- uredospores of P. polysora, 2- eggs of S. frugiperda, 3- corn pollen, 4- artificial diet, and maintained for 10 minutes, to evaluate the food choice and feeding time. The exclusive feeding with S. frugiperda eggs caused low nymph survival (8%), but the combination of P. polysora uredospores + S. frugiperda eggs allowed 58.3% survival. D. luteipes preferred feeding during the nighttime and the most significant proportions of choices by nymphs and adults were for pollen and diet, with adults spending more time eating pollen. These findings indicate that the trophic choices of D. luteipes are relevant to understand its contribution as an agent to control pest insects and fungal diseases in corn.(AU)

Doru luteipes (Scudder, 1876) é um predador onívoro, que encontra na planta do milho diferentes recursos alimentares: ovos de Spodoptera frugiperda (J.E. Smith, 1797), uredósporos de Puccinia polysora (Underw, 1897) e pólen. Para definição da relevância desse predador como agente de controle biológico, conhecer a sobrevivência e preferência alimentar é essencial. Nós hipotetizamos que o comportamento de forrageamento e a capacidade de predação de D. luteipes podem ser afetados quando uma mesma planta oferece ovos, uredósporos e pólen, concomitantemente. A sobrevivência de D. luteipes na fase de ninfa e sua preferência entre os recursos alimentares, frequentemente disponíveis nas plantas de milho, foram determinados. Para verificar a sobrevivência de D. luteipes, ninfas recém eclodidas foram alimentadas exclusivamente com 1- uredósporos de P. polysora, 2- ovos de S. frugiperda, 3- pólen de milho, 4- combinação de uredósporos + ovos e 5- dieta artificial (controle). Em outro experimento, ninfas e adultos de D. luteipes com 24 e 48 horas de jejum foram liberadas individualmente, em recipientes contendo quatro dietas: 1- uredósporos de P. polysora, 2- ovos de S. frugiperda, 3- pólen de milho, 4- dieta artificial e mantidas durante 10 min, sendo avaliados a escolha pelo alimento e o tempo de alimentação. A alimentação exclusiva com ovos de S. frugiperda ocasionou baixa sobrevivência das ninfas (8%), porém a combinação de uredósporos de P. polysora + ovos de S. frugiperda possibilitou sobrevivência de 58,3%. D. luteipes preferiu se alimentar durante o período noturno e as maiores proporções de escolhas das ninfas e dos adultos ocorreram no pólen e na dieta, sendo que os adultos gastaram mais tempo se alimentando de pólen. Estas descobertas indicam que as escolhas tróficas de D. luteipes são relevantes para compreender sua contribuição como agente de controle de insetos-praga e doença fúngicas em milho.(AU)

Feeding behavior of sheep in feedlot and fed with diets containing detoxified castor cake in substitution to the soybean meal

To evaluate the ingestive behavior of sheep confined and fed with substitution levels of soybean meal (SM) by detoxified castor cake (DCC). We adopted a completely randomized design, with four levels of substitution (0; 33; 67 and 100%) with five replicates (sheep). We estimated the percentages of total times of intake of feed, time of rumination, in 'other activities', in idle agreed and in idle sleeping, dividing the day into eight periods (5h00 to 8h00; 8h01 to 11h00; 11h01 to 14h00; 14h01 to 17h00; 17h01 to 20h00; 20h01 to 23h00; 23h01 to 2h00 and 2h01 to 5h00). No interaction was observed between levels of substitution of the SM by DCC and period of the day to go. However, the isolated effect period of the day, there is more time to go from 8h00 to 11h00. The time rumination was not influenced by the levels of substitution of the SM by DCC. As for the variable other activities, the level of 67% DCC was superior to that of 100%. Regarding the variables idle agreed and idle sleeping, were not observed effects of substitution levels of SM by DCC. The DCC provides no changes in the behavior of the sheep, but the period of the day exerts influence on the behavioral pattern of such animals.(AU)

Linking toxicity and predation in a venomous arthropod: the case of Tityus fuhrmanni (Scorpiones: Buthidae), a generalist predator scorpion

Background: Scorpions are arachnids that have a generalist diet, which use venom to subdue their prey. The study of their trophic ecology and capture behavior is still limited compared to other organisms, and aspects such as trophic specialization in this group have been little explored. Methods: In order to determine the relationship between feeding behavior and venom toxicity in the scorpion species Tityus fuhrmanni, 33 specimens were offered prey with different morphologies and defense mechanisms: spiders, cockroaches and crickets. In each of the experiments we recorded the following aspects: acceptance rate, immobilization time and the number of capture attempts. The median lethal dose of T. fuhrmanni venom against the three different types of prey was also evaluated. Results: We found that this species does not have a marked difference in acceptance for any of the evaluated prey, but the number of capture attempts of spiders is higher when compared to the other types of prey. The immobilization time is shorter in spiders compared to other prey and the LD50 was higher for cockroaches. Conclusions: These results indicate that T. fuhrmanni is a scorpion with a generalist diet, has a venom with a different potency among prey and is capable of discriminating between prey types and employing distinct strategies to subdue them.(AU)

Immunocastrated female pigs' social and feeding behaviour

- This study compared the social and feeding behaviour of immunocastrated female pigs using different immunocastration protocols. We used seventy-two gilts from industrial crossbreeding (Agroceres × Topigs) at 15 weeks of age. The pigs were distributed in a randomised design with three treatments. The second dose of the immunocastration vaccine was applied in two groups, six weeks before slaughter (I6) and four weeks before slaughter (I4). A non-immunised group (NI) was used for comparison. Due to the divergence in the time of immunisation between the two groups, an individual comparison of each immunocastrated group with the control group (NI vs. I6 and NI vs. I4) was performed. Social behaviour was analysed through the collection of images by a monitoring camera, and the analysis period was from 6:00 to 18:00 h, with behaviour observed every ten minutes. Feeding behaviour data were collected by the automated FIRE® system. Anti-GnRH immunisation and the different protocols of application did not influence most social and feeding behaviours.(AU)

Monitoring post-release behavioural activity of captive-bred urial (Ovis vignei punjabiensis) at Togh Managara Safari Park Khyber Pakhtunkhwa, Pakistan / Monitoramento da atividade comportamental pós-liberação de urial criado em cativeiro (Ovis vignei punjabiensis) em Togh Managara Safari Park Khyber Pakhtunkhwa, Paquistão

The Punjab urial (Ovis vignei punjabiensis) is a globally threatened wild sheep species. In Pakistan the species is legally protected and bred in captivity to release into natural habitats to reinforce wild populations. Vigilance and feeding are critical to survival, though the degree to which captive-bred animals exhibit these behaviours post-release has rarely been investigated. We monitored the daily behaviours of five adult urial after release and classifying behaviours from 0600 hrs to 1800 hrs, replicating each one-hour period twice and recording four observations of each animal per hour. At the herd level, vigilance and feeding behaviours were exhibited equally. Males and females invested equal time in feeding, males were more vigilant (p = 0.001) and more aggressive (p = 0.010), and females were more restful (p = 0.019) or engaged in grooming (p = 0.044) or locomotion (p = 0.002). Females spent more time feeding than being vigilant (p = 0.002). Males maintained high levels of vigilance throughout the day. Patterns of resting 1300 hrs to 1500 hrs and feeding in early morning and late afternoon were common for both sexes. Behaviours classified as 'other' were exhibited equally between sexes. Our results reveal positive indications of captive-bred urial balancing threat detection and energy acquisition post-release and exhibiting natural behaviours and activity patterns. We encourage assessment of survivorship to evaluate long-term effectiveness of captive breeding and release as a candidate restoration programme.

O Punjab urial (Ovis vignei punjabiensis) é uma espécie de ovelha selvagem ameaçada globalmente. No Paquistão, a espécie é legalmente protegida e criada em cativeiro para ser liberada em habitats para reforçar as populações selvagens. Vigilância e alimentação são essenciais para a sobrevivência, embora o grau de comportamento que os animais criados em cativeiro apresenta raramente tenha sido estudado. Cinco uriais adultos foram monitorados diariamente, após a liberação os comportamentos foram classificados das 6 h às 18 h, replicando cada período de uma hora por duas vezes e registrando quatro observações de cada animal por hora. Em nível do rebanho, os comportamentos de vigilância e alimentação foram exibidos igualmente. Machos e fêmeas investiam tempo igual na alimentação, enquanto os machos eram mais vigilantes (p = 0,001) e mais agressivos (p = 0,010), as fêmeas eram mais repousantes (p = 0,019) ou mais engajadas no asseio (p = 0,044) ou na locomoção (p = 0,002). As fêmeas passam mais tempo se alimentando do que vigiando (p = 0,002). Os machos mantiveram altos níveis de vigilância ao longo do dia. Padrões de descanso de 1300 a 1500 horas e alimentação no início da manhã e no final da tarde eram comuns para ambos os sexos. Comportamentos classificados como "outros" foram exibidos igualmente entre os sexos. Os resultados revelam indicações positivas na detecção de ameaça em relação ao equilíbrio de criação urial em cativeiro e em aquisição de energia pós liberação, exibindo comportamentos naturais e padrões de atividade. Neste estudo, encorajou-se a utilização da avaliação de sobrevivência para avaliar a eficácia em longo prazo da reprodução em cativeiro e soltura como um programa de restauração dos candidatos.

Monitoring post-release behavioural activity of captive-bred urial (Ovis vignei punjabiensis) at Togh Managara Safari Park Khyber Pakhtunkhwa, Pakistan / Monitoramento da atividade comportamental pós-liberação de urial criado em cativeiro (Ovis vignei punjabiensis) em Togh Managara Safari Park Khyber Pakhtunkhwa, Paquistão

The Punjab urial (Ovis vignei punjabiensis) is a globally threatened wild sheep species. In Pakistan the species is legally protected and bred in captivity to release into natural habitats to reinforce wild populations. Vigilance and feeding are critical to survival, though the degree to which captive-bred animals exhibit these behaviours post-release has rarely been investigated. We monitored the daily behaviours of five adult urial after release and classifying behaviours from 0600 hrs to 1800 hrs, replicating each one-hour period twice and recording four observations of each animal per hour. At the herd level, vigilance and feeding behaviours were exhibited equally. Males and females invested equal time in feeding, males were more vigilant (p = 0.001) and more aggressive (p = 0.010), and females were more restful (p = 0.019) or engaged in grooming (p = 0.044) or locomotion (p = 0.002). Females spent more time feeding than being vigilant (p = 0.002). Males maintained high levels of vigilance throughout the day. Patterns of resting 1300 hrs to 1500 hrs and feeding in early morning and late afternoon were common for both sexes. Behaviours classified as 'other' were exhibited equally between sexes. Our results reveal positive indications of captive-bred urial balancing threat detection and energy acquisition post-release and exhibiting natural behaviours and activity patterns. We encourage assessment of survivorship to evaluate long-term effectiveness of captive breeding and release as a candidate restoration programme.(AU)

O Punjab urial (Ovis vignei punjabiensis) é uma espécie de ovelha selvagem ameaçada globalmente. No Paquistão, a espécie é legalmente protegida e criada em cativeiro para ser liberada em habitats para reforçar as populações selvagens. Vigilância e alimentação são essenciais para a sobrevivência, embora o grau de comportamento que os animais criados em cativeiro apresenta raramente tenha sido estudado. Cinco uriais adultos foram monitorados diariamente, após a liberação os comportamentos foram classificados das 6 h às 18 h, replicando cada período de uma hora por duas vezes e registrando quatro observações de cada animal por hora. Em nível do rebanho, os comportamentos de vigilância e alimentação foram exibidos igualmente. Machos e fêmeas investiam tempo igual na alimentação, enquanto os machos eram mais vigilantes (p = 0,001) e mais agressivos (p = 0,010), as fêmeas eram mais repousantes (p = 0,019) ou mais engajadas no asseio (p = 0,044) ou na locomoção (p = 0,002). As fêmeas passam mais tempo se alimentando do que vigiando (p = 0,002). Os machos mantiveram altos níveis de vigilância ao longo do dia. Padrões de descanso de 1300 a 1500 horas e alimentação no início da manhã e no final da tarde eram comuns para ambos os sexos. Comportamentos classificados como "outros" foram exibidos igualmente entre os sexos. Os resultados revelam indicações positivas na detecção de ameaça em relação ao equilíbrio de criação urial em cativeiro e em aquisição de energia pós liberação, exibindo comportamentos naturais e padrões de atividade. Neste estudo, encorajou-se a utilização da avaliação de sobrevivência para avaliar a eficácia em longo prazo da reprodução em cativeiro e soltura como um programa de restauração dos candidatos.(AU)

Food habits of indian crested porcupine (Hystrix indica) (Kerr 1792), in district Bagh, Azad Jammu and Kashmir / Hábitos alimentares do porco-espinho de crista indiano (Hystrix indica) (Kerr 1972) nos distritos de Bagh, Azad Jammu e Caxemira

The Indian Crested Porcupine (Hystrix indica) is classified as an agricultural pest species. It feeds on plants and crops; hence, it is responsible for massive financial losses worldwide. The current study was conducted to assess the diet composition of Indian Crested Porcupine in District Bagh, Azad Jammu and Kashmir (AJ&K). Thus, fecal samples were collected and examined from different sampling sites. Reference slides of the material collected from the study area were prepared for identification of dietary components in fecal pellets. A total of 80 fecal samples were collected and processed. Percent relative frequencies (P.R.F.) were calculated for each plant species recovered from pellets. Data revealed that Indian Crested Porcupine consumed 31 plant species in its diet, among them Zea mays (34.31±7.76) was the most frequently selected species followed by Rumex obtusifolius (15.32±2.57) and Melia azedarach (12.83±4.79). The study revealed that the greatest diversity of (n=20) plant species were consumed in summer season while minimum (n=13) species were used during winter. Among the parts of plants, stem was highly consumed in spring (57.2%) as compared to seed in fall (36.7%) while spikes and leaf were the least recovered parts from the fecal matter. The Berger-Parker diversity index showed highly diversified food (10.92) in the summer time of the year as compared to the autumn season (2.95). This study provides a baseline for the diet preference of this pest in the study area. Based on current findings, a detailed investigation on damage assessment, exploration, habitat use and management of Indian Crested Porcupine in AJ&K has been recommended.

O porco-espinho de crista indiano (Hystrix indica) é classificado como uma espécie de praga agrícola. Alimenta-se de plantas e colheitas; portanto, é responsável por enormes perdas financeiras em todo o mundo. O estudo atual foi realizado para avaliar a composição da dieta do porco-espinho de crista indiano nos distritos de Bagh, Azad Jammu e Caxemira (AJ&K). Assim, amostras fecais foram coletadas e examinadas em diferentes locais de amostragem. Lâminas de referência do material coletado na área de estudo foram preparadas para identificação dos componentes da dieta em pellets fecais. Um total de 80 amostras fecais foi coletado e processado. As frequências relativas percentuais (P.R.F.) foram calculadas para cada espécie de planta recuperada de pelotas. Os dados revelaram que o porco-espinho indiano consumiu 31 espécies de plantas em sua dieta, entre elas Zea mays (34,31 ± 7,76) foi a espécie mais selecionada, seguida por Rumex obtusifolius (15,32 ± 2,57) e Melia azedarach (12,83 ± 4,79). O estudo revelou que a maior diversidade de (n = 20) espécies de plantas foi consumida no verão, enquanto o mínimo (n = 13) espécies foi utilizado durante o inverno. Entre as partes das plantas, o caule foi muito consumido na primavera (57,2%) em relação à semente no outono (36,7%), enquanto a espiga e a folha foram as partes menos recuperadas da matéria fecal., O índice de diversidade de Berger-Parker mostrou alimentos altamente diversificados (10,92) no verão do ano em comparação com o outono (2,95). Este estudo fornece uma linha de base para a preferência alimentar dessa praga na área de estudo. Com base nas descobertas atuais, uma investigação detalhada sobre avaliação de danos, exploração, uso de habitat e gerenciamento de porco-espinho de crista indiano em AJ&K foi recomendada.

Production, intake, and feeding behavior of dairy goats fed alfalfa via grazing and cassava

This study examined the replacement of maize and soybean meal with cassava chips and alfalfa grazing, respectively. Twelve lactating Anglo-Nubian goats were kept on a Panicum maximum cv. Tobiatã pasture. The experiment was laid out in a Latin square design in which the following diets were tested: ground maize + soybean meal, cassava chips + soybean meal, ground maize + alfalfa grazing, and cassava chips + alfalfa grazing. The evaluated variables were feed intake, daily weight gain, milk yield and composition, and feeding behavior of the goats as well as production costs. Cassava chips and grazed alfalfa influenced the intakes of dry matter, crude protein, neutral detergent fiber, and total digestible nutrients. However, milk yield, body weight, and body score did not change. There was no diet effect on the proportions of protein, solids-not-fat, somatic cell count, or urea nitrogen in the milk. Treatments influenced the levels of fat, lactose, and total solids in milk, with the highest fat levels achieved with diets containing alfalfa. Grazing, rumination, and idle times and time spent interacting with other goats were not influenced by diets. The evaluated feedstuffs improved feed efficiency and reduced production costs. Therefore, cassava chips and alfalfa can replace certain ingredients without impairing the production performance of goats, but rather improving the profit of the producer.(AU)

Ultrastructural analysis of the antennae of Hemilucilia segmentaria (Diptera: Calliphoridae), a blowfly of forensic importance

Hemilucilia segmentaria (Fabricius, 1805) is a Neotropical blowfly species of forensic importance, with necrophagous and asynanthropic habits. In this study, the antennal ultrastructure of H. segmentaria was examined using scanning electron microscopy. The three antennal segments are covered by microtrichia. Sensilla chaetica were detected only on the scape and pedicel. Setiferous plaques and a pedicellar button were observed on the pedicel. Four types of sensilla were found on the postpedicel, including s. trichoidea, s. basiconica (subtype I, II and III), s. coeloconica subtype I, and sensory pits with s. coeloconica subtype II. This is the first time that the fine structure of the antennae of H. segmentaria was studied. Our results constitute a solid base for research on comparative and functional morphology in H. segmentaria and other blowflies.(AU)

Filling gaps in the ecological knowledge on Auchenipteridae catfishes (Ostariophysi: Siluriformes): first data for Trachelyichthys exilis / Preenchendo lacunas no conhecimento ecológico de bagres Auchenipteridae (Ostariophysi: Siluriformes): primeiros dados para Trachelyichthys exilis

The feeding habit of Trachelyichthys exilis is described for the first time, with additional comments on its growth type and size at sexual maturity. We analyzed 42 specimens from the Amanã Reserve (Amazonas state, Brazil) collected with a seine net amidst stands of floating herbaceous vegetation during the dry season of 2002. Stomach contents revealed a predominantly carnivorous habit (tending to piscivorous), an isometric growth type, and size at maturity around 5.5 cm standard length. Based on the biological characteristics of the consumed prey, we presume that T. exilis forage actively during the twilight/night around and among the root tangle of herbaceous vegetation, preying upon nocturnally active animals such as small fishes and aquatic invertebrates, and/or close to the water surface, where diurnally active prey usually rests. Our findings provide essential information to fill knowledge gaps on the natural history of auchenipterid catfishes, especially on trophic ecology.(AU)

O hábito alimentar de Trachelyichthys exilis é descrito pela primeira vez, com comentários adicionais sobre seu tipo de crescimento e tamanho de maturação sexual. Nós analisamos 42 espécimes da Reserva Amanã (Amazonas, Brasil) coletados com rede de cerco em bancos flutuantes de herbáceas durante a estação seca de 2002. Conteúdos estomacais revelaram um hábito predominantemente carnívoro (tendendo a piscívoro), um tipo de crescimento isométrico e tamanho de maturação sexual em torno de 5,5 cm de comprimento padrão. Com base nas características biológicas das presas consumidas, presumimos que T. exilis forrageia ativamente durante o crepúsculo/noite ao redor e entre as raízes dos bancos flutuantes, onde captura presas noturnas como pequenos peixes e invertebrados aquáticos, e/ou próximo à superfície da água, onde geralmente repousam as presas diurnas. Nossos resultados fornecem informações essenciais para preencher lacunas de conhecimento sobre a história natural de bagres auchenipterídeos, especialmente sobre a ecologia trófica.(AU)

South American Sea Lions Otaria flavescens, a good indicator of relative spatial and temporal changes in the distribution and abundance of marine resources? / Lobos marinos de un pelo sudamericanos Otaria flavescens ¿buenos indicadores de cambios espaciales y temporales relativos en la distribución y abundancia de los recursos marinos?

Oceanographic fluctuations and changes in ocean productivity directly affect the abundance and distribution of prey species, which in turn, affect the population status of their predators. In order to have a better understanding of this predator-prey relationship, the aim of the present study was to analyze the diet of the South American Sea Lion Otaria flavescens (Shaw, 1800) in two rookeries of the San Matias Gulf, in northern Patagonia (Argentina). A total of 52 scat samples of O. flavescens were collected in the Promontorio Belén colony and 35 in Caleta de los Loros during the late spring of 2011. The analysis of the samples indicated that at both localities fish occurred in 100% of scats with prey remains, followed by cephalopods (32.3%) and crustaceans (21.4%). The fish Raneya brasiliensis (Kaup, 1856) constituted the main prey taxon, both in terms of occurrence and numerical abundance. The octopod Octopus tehuelchus (d' Orbigny, 1834) and squids of the genus Doryteuthis constituted the dominant cephalopod prey. It is concluded that sea lions focused their foraging activity on those species of demersal and benthic habits, associated with coastal and shelf waters. Furthermore, this study reports for the first time the feeding habits of the South American Sea Lion colony of Promontorio Belén, second in population size of Río Negro province.

Las fluctuaciones oceanográficas y los cambios en la productividad del océano afectan directamente a la abundancia y distribución de las especies presa, que a su vez afectan al estado de la población de sus predadores. Con el fin de tener una mejor comprensión de esta relación predador-presa, el objetivo del presente trabajo fue analizar la dieta del lobo marino de un pelo sudamericano Otaria flavescens (Shaw, 1800) en dos apostaderos del Golfo San Matias, en Patagonia norte (Argentina). Se colectaron un total de 52 muestras de fecas de O. flavescens en el apostadero de Promontorio Belén y 35 en Caleta de los Loros durante la primavera tardía de 2011. El análisis de las muestras indicó que en ambas localidades los peces ocurrieron en el 100% de las muestras con remanentes alimentarios, seguidos por cefalópodos (32.3%) y crustáceos (21.4%). El pez Raneya brasiliensis (Kaup, 1856) constituyó el principal taxón presa, tanto en términos de ocurrencia como de abundancia numérica. El octópodo Octopus tehuelchus (d' Orbigny, 1834) y los calamares del género Doryteuthis constituyeron los cefalópodos presa dominantes. Se concluye que los lobos marinos centraron su actividad forrajera en aquellas especies de hábitos demersales y bentónicos, asociado a aguas costeras y de plataforma. Además, este estudio analiza por primera vez los hábitos alimenticios de la colonia de lobos marinos de un pelo sudamericanos en el apostadero de Promontorio Belén, segunda en tamaño poblacional de la provincia de Río Negro.

Productive performance and ingestive behavior of crossbred heifers in integrated livestock-forest systems

The present study aimed to assess the productive performance, ingestive behavior, and thermal condition of crossbred beef heifers in an integrated livestock-forest (ILF) system with different tree-planting designs. Seventy-two ½ Angus ½ Nellore heifers were divided in three treatments­conventional system (CS) without eucalyptus, integrated livestock system with 187 eucalyptus ha−1 (ILF-1L), and integrated livestock system with 446 eucalyptus ha−1 (ILF-3L)­, in the winter and summer. Total dry mass of forage (TDM), chemical analysis of Marandu palisadegrass, average daily gain (ADG), stocking rate (SR), weight gain per area (WGA), behavior, microclimatic variables, and thermal comfort indexes were evaluated. A higher percentage of crude protein and lower TDM and SR were observed in ILF-1L and ILF-3L, with no differences for ADG and WGA. Ambient temperature, temperature-humidity index, black globe-humidity index, and radiant heat load were better in ILF-1L and ILF-3L. Heifers in ILF-1L and ILF-3L carried out grazing, resting, rumination, and other activities in the shade. In the summer, heifers in ILF-1L and ILF-3L spent less time resting and more time in rumination and performing other activities than those in the CS. In conclusion, the better forage quality in ILF-1L and ILF-3L does not alter the performance of heifers or WGA, despite the decreased TDM and SR. Furthermore, ILF-1L and ILF-3L provide better thermal comfort, and heifers that do not have access to shade decrease their activities as a strategy to reduce the use of energy for thermal regulation process.(AU)

Exogenous enzymes in sheep diet: nutritional and physiological parameters

The objective of this study was to evaluate the effect of adding exogenous enzymes to the diet on nutrient intake and digestibility, nitrogen balance, ingestive behavior and rumen movement of ewe lambs. Five ewe lambs, Dorper x Santa Inês crossbred, with an average age of 7 ± 1 months, average weight of 36.40 ± 2.36 kg were assigned in a 5x5 Latin square design. The treatments consisted of adding exogenous enzymes to the concentrate: Allzyme, Fibrozyme®, Amaize®, Mix and Control. Analyses of variance were applied and means were compared by the SNK test, and non-parametric Kruskal Wallis test at 5% significance. The dry matter intake in relation to body weight, crude protein intake and nitrogen intake were higher with the use of amylolytic enzyme compared to the other treatments (p <0.05). Nitrogen balance was higher with the use of amylolytic enzymes and the Allzyme® enzyme complex (p <0.05). A longer time spent in total chewing was observed with the inclusion of fibrolytic and amylolytic enzymes without changes in rumen movement. The use of exogenous enzymes promotes better use of nutrients, with high digestibility of dry matter, neutral detergent fiber and crude protein.(AU)

Impact of Pre-transport Feed Withdrawal on Welfare and Meat Characteristics of European Quails

The objective of this study was evaluate whether pretransport feed withdrawal affects welfare, carcass, and meat characteristics of European quails. A total of 120 European quails were used, with an initial age of 15 days. Bird feed was removed before transport to the slaughterhouse at the following times, which characterised the different treatments: zero hours, three hours, six hours, nine hours and twelve hours. The transport took 54 minutes to cover 27 kilometres to a commercial slaughterhouse. The birds were slaughtered at 41 days of age. During bleeding, blood samples were collected. Blood glucose, total protein, albumin, uric acid, and corticosterone concentrations were measured. The carcasses and meat characteristics were measured. Poultry body weight decreased and blood glucose concentrations increased with the increase in feed withdrawal time. The treatments did not affect carcass weights. Carcass yields after three hours fasting were similar to those in the six hours and nine hours groups, indicating that gastrointestinal tracts were empty after the third hour. Meat quality was negatively affected (pH, lightness, water holding capacity, cooking loss) by the increase in feed withdrawal time; integrated parameters that characterise dark, firm, dry meat. Pre transport feed withdrawal time should be three hours to empty the gastrointestinal tract and minimise losses in meat quality of European quails. It is necessary to adjust feed withdrawal so that it does not exceed this time, since there is no technical justification for supporting it.(AU)

Assessment of the drinker type on water intake and toy type on behaviour of nursery piglets / Avaliação do tipo de bebedouro sobre o consumo hídrico e o tipo de brinquedo sobre o comportamento de leitões de creche

This study was conducted to assess the effect of two types of drinkers on water intake (WI) and two types of toys on behavioural observations of piglets in the nursery phase. A total of 72 crossbred entire male piglets (Landrace × Large White, Agroceres♂ and DanBred♀), weaned at 21 days of age, with an average initial body weight (AIBW) of 6.75 ± 0.19 kg were used. Two statistical models were fitted for WI. In model I, animals were distributed in a completely randomized design (CRD) with a split-plot in time, composed of six treatments (2 types of drinkers × 3 experimental phases), eight replications (days of sampling), and 36 animals/experimental unit (EU), without using AIBW as a covariate. In model II, animals were distributed in a CRD with two treatments (fixed or pendular nipple drinkers), eight replications (days of sampling) and 36 animals/EU, with use of AIBW as a covariate. For assessment of behavioural observations, the model was adjusted in a randomized block design, consisting of four treatments [two types of toys (metal chain and plastic bottle) × two daytime periods (morning and afternoon)], nine replications and three blocks constituted in time (days), totalling four pigs/ EU. The results indicated an effect on animal daily WI in the starter phase in model I (p < 0.000) and II (p = 0.006). There was an effect (p ≤ 0.05) of toy type for behavioural observations, in which pigs showed a greater proportion of fighting and belly nosing when they had the bottle toy. However, animals spent more time (p ≤ 0.05) eating, drinking water, overlapping, and playing with the toy when they had access to the chain toy. There was an increase (p ≤ 0.05) in sleeping behaviour in the morning period, as well as greater (p ≤ 0.05) behavioural activity in the afternoon period. In conclusion, WI of nursery piglets was positively influenced by growth phase, and the fixed drinker stimulated greater WI in piglets in the starter phase. In addition, the metal chain, as an environmental enrichment, promoted improvements in feeding behaviour and WI, reducing fighting events, and the afternoon period had increased behavioural observations overall.(AU)

Um estudo foi conduzido com o objetivo de avaliar dois tipos de bebedouros e seus efeitos sobre o consumo hídrico (CH) e dois tipos de brinquedos sobre os eventos comportamentais de leitões na fase de creche. Um total de 72 leitões machos inteiros mestiços (Landrace × Large White, Agroceres♂ e DanBred♀), desmamados aos 21 dias de idade e peso corporal inicial médio (PCIM) de 6,75 ± 0,19 kg. Para o CH foram ajustados dois modelos estatísticos. No modelo I, os animais foram distribuídos em um delineamento inteiramente casualizado (DIC) e esquema de parcelas subdivididas no tempo, composto de seis tratamentos (2 tipos de bebedouros × 3 fases experimentais), oito repetições (dias de amostragem) e 36 animais/unidade experimental (UE) e sem utilização de PCIM como covariável. No modelo II, os animais foram distribuídos em um DIC aninhado com dois tratamentos (bebedouro tipo chupeta fixo ou pendular), oito repetições (dias de amostragem) e 36 animais/UE, com utilização de PCIM como covariável. Para a avaliação dos eventos comportamentais, foi ajustado um modelo em delineamento de blocos casualizados, constituído de quatro tratamentos [dois tipos de brinquedos (corrente de metal e garrafa plástica) × dois períodos do dia (matutino e vespertino)], nove repetições e três blocos constituídos no tempo (dias), totalizando quatro suínos/UE. Os resultados indicaram efeito no CH diário animal na fase inicial no modelo I (p < 0,000) e II (p = 0,006). Houve efeito (p ≤ 0,05) de tipo de brinquedo para as observações comportamentais, em que os suínos apresentaram maior proporção de brigas e empurrando a barriga quando tiveram o brinquedo de garrafa. Entretanto, os animais passaram maior tempo (p ≤ 0,05) comendo, bebendo água, montando outro animal e brincando com o brinquedo quando tiveram acesso ao brinquedo de corrente. Houve um aumento (p ≤ 0,05) no comportamento de dormir no período matutino, bem como os suínos apresentaram maior (p ≤ 0,05) atividade comportamental no período vespertino. Em conclusão, o consumo hídrico de leitões em fase de creche é influenciado positivamente pela fase de crescimento e o bebedouro fixo estimulou um maior consumo de água em leitões na fase inicial. Em adição, o tipo de enriquecimento ambiental corrente promoveu melhorias no comportamento alimentar e de ingestão de água, reduzindo eventos de brigas, bem como o período vespertino aumentou os eventos comportamentais em geral.(AU)