Population level genetic divergence and phylogenetic placement of Mexican shortfin mollies (Mollienesia: Poecilia: Poeciliidae)

Mexico is a megadiverse region with a complex geological history, but it remains unclear to what extent the distribution of freshwater fish has been influenced by geographic barriers. This study examines the population level genetic divergence and phylogenetic relationships of species in the shortfin group of the subgenus Mollienesia (genus Poecilia), a group of live-bearing fishes that are widely distributed across Mexico, with sampling at a small geographic scale. Samples from over 50 locations were analyzed for six species by using phylogenetic and haplotype network approaches to assess genetic diversity across geographic ranges and to refine the distributions of species in this group. The results indicate that Mexican species have diversified following multiple, independent invasions from Middle America. Two species found north of the Trans-Mexican Volcanic Belt (TMVB) and one transversal species exhibited weak phylogenetic structure, likely due to the lack of physiographic barriers, recent colonization, and high dispersal rates among regions. In contrast, three species found south of the TMVB exhibited strong phylogenetic structure, reflecting a longer presence in the area and multiple physiographic barriers that isolated populations. This study identified mechanisms driving divergence and speciation, expanded the known range of several species, and resolved taxonomic uncertainties of populations.(AU)

México es una región megadiversa con una historia geológica compleja, pero se desconoce el nivel de influencia de las barreras geográficas sobre las distribuciones de los peces dulceacuícolas. Este estudio examina las relaciones filogenéticas, a escala geográfica pequeña, de las especies del grupo de aletas cortas del subgénero Mollienesia (género Poecilia), un grupo de peces vivíparos ampliamente distribuidos en México. Se analizaron muestras de seis especies en más de 50 localidades, utilizando métodos filogenéticos y de redes de haplotipos, para evaluar la diversidad genética y precisar las distribuciones de especies en este grupo. Los resultados indican que las especies mexicanas se han diversificado a partir de múltiples invasiones independientes desde Mesoamérica. Se detectó estructura filogenética débil en dos especies distribuidas al norte del Eje Neovolcánico y una especie que atraviesa el Eje Neovolcánico, posiblemente debido a la ausencia de barreras fisiográficas, colonización reciente y altas tasas de dispersión entre regiones. En contraste, se detectaron niveles altos de estructura filogenética en tres especies distribuidas del Eje Neovolcánico, lo que refleja una presencia más prolongada en el área y la existencia de múltiples barreras fisiográficas que aislaron a las poblaciones. Este estudio identificó mecanismos que promueven la divergencia y la especiación, expandió el rango conocido de varias especies y resolvió incertidumbres taxonómicas de algunas poblaciones.(AU)

Cysticercosis in free-ranging agoutis (Dasyprocta leporina) in the Eastern Brazilian Amazon / Cisticercose em cutias (Dasyprocta leporina) silvestres na Amazônia Oriental brasileira

The study describes the occurrence of cysticercosis in liver of 22 wild agoutis (Dasyprocta leporina) in the Brazilian Amazon. The phylogenetic analysis and microscopic characteristics of metacestodes in liver tissue sections, associated with the geographic distribution of the intermediate hosts indicated that a possibly novel Taenia sp. metacestode caused the parasitism. Additionally, two cases of hepatic co-infection by Taenia sp., Calodium sp. and Echinococcus oligarthra were also observed among the analyzed animals. The results point to the need for a better understanding of hepatotropic parasites among wild rodents in the Brazilian Amazon.(AU)

O estudo descreve a ocorrência de cisticercose no fígado de 22 cutias (Dasyprocta leporina) silvestres da Amazônia brasileira. A análise filogenética e as características microscópicas dos metacestódeos em cortes histológicos de fígado, associadas à distribuição geográfica do hospedeiro intermediário, indicaram que, possivelmente, uma nova espécie de Taenia sp. Causou o parasitismo. Adicionalmente, dois casos de co-infecção por Taenia sp., Calodium sp. e Echinococcus oligarthra também foram observados entre os animais avaliados. Os resultados apontam para a necessidade de um melhor entendimento dos parasitas hepatotrópicos entre roedores selvagens da Amazônia brasileira.(AU)

Bovine landscape genetics in Brazil

ABSTRACT The aim of this study was to explore spatial genetic structure patterns in cattle breeds adapted to local conditions in Brazil. We georeferenced 876 animals of ten breeds raised in Brazil kept in the Genebank of Brazilian Agricultural Research Corporation (Embrapa) by sample locations using the QGIS 2.4.0 software. The Mantel tests, spatial autocorrelation, and Monmonier tests were performed. The distances for spatial correlation tests ranged from 5 to 15 classes. The results indicated genetic discontinuities in cattle breeds from the Midwest, South, and Southeast of the country. Correlation between genetic and geographic distance was low, but significant. The Monmonier Maximum Distance Algorithm indicated an initial subdivision of Curraleiro and then Pantaneiro from the other breeds. In another subdivision, Criollo, Mocho Nacional, and Caracu were grouped. Genetic discontinuity was observed beyond 431 km, the minimum sampling distance between populations for conservation purposes.

Bovine landscape genetics in Brazil

The aim of this study was to explore spatial genetic structure patterns in cattle breeds adapted to local conditions in Brazil. We georeferenced 876 animals of ten breeds raised in Brazil kept in the Genebank of Brazilian Agricultural Research Corporation (Embrapa) by sample locations using the QGIS 2.4.0 software. The Mantel tests, spatial autocorrelation, and Monmonier tests were performed. The distances for spatial correlation tests ranged from 5 to 15 classes. The results indicated genetic discontinuities in cattle breeds from the Midwest, South, and Southeast of the country. Correlation between genetic and geographic distance was low, but significant. The Monmonier Maximum Distance Algorithm indicated an initial subdivision of Curraleiro and then Pantaneiro from the other breeds. In another subdivision, Criollo, Mocho Nacional, and Caracu were grouped. Genetic discontinuity was observed beyond 431 km, the minimum sampling distance between populations for conservation purposes.(AU)

Characterization and genetic diversity in Paspalum notatum Flügge accessions: Morphological and geographical distance

The objective of this work was to describe morphology and grouping of Paspalum notatum accessions, based on multicategorical data which discards the redundant variables for quantification of genetic diversity. We also tested the hypothesis that geographical distance was correlated with morphological divergence. In our study, multivariate analyzes successfully demonstrated the geographic and morphological variability of the P. notatum accessions characterized. Many of these evaluated accessions can be included in future genetic improvement programs. Based on two methodologies for discarding variables, it was possible to identify the potentially important morphological characteristics from genetic diversity studies and characterize new accessions aimed at improving forage and seed production. The methodologies used to discard variables are biometric tools that can be used successfully in future plant breeding programs, especially when a large number of traits and accessions are being evaluated. Although significant, geographic distance had a low association with morphological traits. This indicated the need to use other characteristics, such as forage and seed yield, in addition to molecular analysis. Our analyzes showed genetic variability in P. notatum for all the characteristics studied.(AU)

Bovine landscape genetics in Brazil

The aim of this study was to explore spatial genetic structure patterns in cattle breeds adapted to local conditions in Brazil. We georeferenced 876 animals of ten breeds raised in Brazil kept in the Genebank of Brazilian Agricultural Research Corporation (Embrapa) by sample locations using the QGIS 2.4.0 software. The Mantel tests, spatial autocorrelation, and Monmonier tests were performed. The distances for spatial correlation tests ranged from 5 to 15 classes. The results indicated genetic discontinuities in cattle breeds from the Midwest, South, and Southeast of the country. Correlation between genetic and geographic distance was low, but significant. The Monmonier Maximum Distance Algorithm indicated an initial subdivision of Curraleiro and then Pantaneiro from the other breeds. In another subdivision, Criollo, Mocho Nacional, and Caracu were grouped. Genetic discontinuity was observed beyond 431 km, the minimum sampling distance between populations for conservation purposes.

Population structuration and chromosomal features homogeneity in Parodon nasus (Characiformes: Parodontidae): A comparison between Lower and Upper Paraná River representatives

The ichthyofauna of the La Plata hydrographic basin is divided into Upper and Lower Paraná River systems due to the geographic isolation of the Sete Quedas waterfalls, currently flooded by the lake of the Itaipu dam. In Parodontidae, pairs of species, or groups of cryptic species were described between these systems. Although genetic isolation and speciation have already been proposed in other species in the group, Parodon nasus has been maintained as a valid species and distributed throughout the La Plata river basin. In this perspective, specimens of P. nasus from four different sampling sites in the Upper and Lower Paraná River systems were compared regarding the karyotypes, molecular analyzes of population biology and species delimitation to investigate their genetic and population isolation in the La Plata river basin. Despite a geographic barrier and the immense geographic distance separating the specimens sampled from the Lower Paraná River system compared to those from the Upper Paraná River, the data obtained showed P. nasus as a unique taxon. Thus, unlike other species of Parodontidae that showed diversification when comparing the groups residing in the Lower versus Upper Paraná River, P. nasus showed a population structure and a karyotypic homogeneity.(AU)

A ictiofauna do sistema hidrográfico La Plata é dividida em alto e baixo rio Paraná devido ao isolamento geográfico dos Saltos das Sete Quedas há 22 milhões de anos, atualmente inundado pelo lago da represa da Usina de Itaipu. Em Parodontidae, espécies pares ou grupos de espécies crípticas foram descritos entre esses sistemas. Contudo, embora o isolamento genético e especiação já tenham sido propostos em outras espécies do grupo, Parodon nasus tem sido mantido como espécie válida e distribuída em toda a bacia do rio La Plata. Nessa perspectiva, exemplares de P. nasus de quatro diferentes pontos de amostragem nos sistemas do alto e baixo rio Paraná foram comparados quanto ao arranjo dos cariótipos, análises moleculares de biologia populacional e delimitação de espécies, afim de investigar seu isolamento genético e populacional na bacia do rio La Plata. Apesar da barreira geográfica e imensa distância geográfica separando os exemplares amostrados no sistema baixo rio Paraná em comparação àqueles do alto rio Paraná, os dados obtidos demonstraram P. nasus como único táxon válido. Dessa forma, diferentemente de outras espécies de Parodontidae que demonstraram diversificação quando comparados grupos pares residentes no baixo e alto rio Paraná, P. nasus demonstrou estruturação populacional e homogeneidade cariotípica.(AU)

Uncertainties and risks in delimiting species of Cambeva (Siluriformes: Trichomycteridae) with single-locus methods and geographically restricted data

Cambeva contains species with complex taxonomy or poorly delimitated in terms of morphology and geopraphic distribution. We conducted an extensive review of Cambeva populations from coastal drainages of Southern to Southeastern Brazil to evaluate species geographic limits with an integrative analysis including morphological and molecular data (COI). We test if two single-locus methods, Bayesian Poisson Tree Processes (bPTP) and Generalized Mixed Yule Coalescent (GMYC), are efficient to delimit species boundaries in Cambeva by the comparison with the diagnosable morphological units. Using GMYC, we also evaluated the combination of tree and molecular clock priors to reconstruct the input phylogeny and assessed how well the implemented model fitted our empirical data. Eleven species were identified using a morphological diagnosability criterion: Cambeva balios, C. barbosae, C. botuvera, C. cubataonis, C. davisi, C. guaraquessaba, C. iheringi, C. tupinamba, and C. zonata and two treated as undescribed species. In contrast with previous knowledge, many of them have wider distribution and high intraspecific variation. Species delimitation based on single-locus demonstrated incongruences between the methods and strongly differed from the morphological delimitation. These disagreements and the violation of the GMYC model suggest that a single-locus data is insufficient to delimit Cambeva species and the failure may be attributable to events of mitochondrial introgression and incomplete lineage sorting.(AU)

Cambeva contém espécies com taxonomia complexa ou mal delimitadas em termos morfológicos e de distribuição geográfica. Realizamos uma extensa revisão de populações de Cambeva das drenagens costeiras do Sul ao Sudeste do Brasil para avaliar os limites das espécies com uma análise integrativa incluindo dados morfológicos e moleculares (COI). Testamos se dois métodos de locus único, Implementação Bayesiana dos Processos da Árvore de Poisson (bPTP) e Coalescente de Yule Misto Generalizado (GMYC), são eficientes para delimitar os limites das espécies em Cambeva pela comparação com as unidades morfológicas diagnosticáveis. Usando o GMYC, também avaliamos a combinação de árvores e relógios moleculares para reconstruir a filogenia e avaliamos o quão bem o modelo implementado se ajustava aos nossos dados empíricos. Foram identificadas 11 espécies usando o critério morfológico: Cambeva balios, C. barbosae, C. botuvera, C. cubataonis, C. davisi, C. guaraquessaba, C. iheringi, C. tupinamba e C. zonata e duas tratadas como espécies não-descritas. Em contraste com o conhecimento prévio, muitas delas têm distribuição mais ampla e alta variação intraespecífica. A delimitação das espécies baseada em locus único demonstrou incongruências entre os métodos e diferiu fortemente da delimitação morfológica. Essas discordâncias e a violação do modelo GMYC sugerem que os dados de locus único são insuficientes para delimitar as espécies de Cambeva e a falha pode ser atribuída a eventos de introgressão mitocondrial e sorteio incompleto da linhagem.(AU)

Morphological and molecular characterization of Austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the Ivaí River, Brazil / Caracterização morfológica e molecular de metacercárias de Austrodiplostomum compactum em olhos e cérebros de peixes do Rio Ivaí, Brasil

Abstract Austrodiplostomum spp. (Platyhelminthes: Digenea) are endoparasites with a broad geographic distribution in South America. During the larval stage, they parasitize the eyes, brains, muscles, gill, kidneys and swim bladder of a wide variety of fishes. The metacercariae of Austrodiplostomum spp. have several morphological characteristics during development, but are very similar among species, which makes it necessary to use molecular tools to contribute to the elucidation during the larval stage. The objective of this study was to perform morphological and molecular analyses of Austrodiplostomum sp. found in specimens of Hypostomus sourced from the Ivaí River in the state of Paraná, Brazil. Of the 93 analyzed specimens (H. hermanni [n = 50], H. albopunctatus [n = 9], Hypostomus sp. 1 [n = 24], and Hypostomus sp. 2 [n = 10]), 60 were parasitized. A total of 577 Austrodiplostomum sp. metacercariae was collected from the infected hosts; DNA from seven of these samples was extracted, amplified, and sequenced. The morphological data associated with the genetic distance values and the relationships observed in the COI gene tree, indicate that all metacercariae were A. compactum. This is the first record of A. compactum parasitizing H. hermanni, H. albopunctatus, Hypostomus sp. 1, and Hypostomus sp. 2 in the Ivaí River.

Resumo Austrodiplostomum spp. (Platyhelminthes: Digenea) são endoparasitos com uma ampla distribuição geográfica na América do Sul. Durante a fase larval, parasitam os olhos, cérebros, músculos, brânquias, rins e bexiga natatória de uma grande variedade de peixes. As metacercárias de Austrodiplostomum spp. apresentam várias características morfológicas durante o desenvolvimento, as quais são muito semelhantes entre as espécies, o que torna necessário o uso de ferramentas moleculares para contribuir para a elucidação durante a fase larval. O objetivo deste estudo foi realizar análises morfológicas e moleculares de Austrodiplostomum sp. encontradas em espécimes de Hypostomus provenientes do rio Ivaí, no Paraná, Brasil. Dos 93 espécimes analisados (H. hermanni [n = 50], H. albopunctatus [n = 9], Hypostomus sp. 1 [n = 24], e Hypostomus sp. 2 [n = 10]), 60 foram parasitados. Um total de 577 metacercárias de Austrodiplostomum foram coletadas dos hospedeiros infectados; o DNA de sete dessas amostras foi extraído, amplificado e sequenciado. Os dados morfológicos, associados aos valores de distância genética e as relações observadas na árvore gênica do COI, indicam que todas as metacercárias são A. compactum. Este é o primeiro registo de A. compactum parasitando H. hermanni, H. albopunctatus, Hypostomus sp. 1, e Hypostomus sp. 2 no rio Ivaí.

Vampire bat Desmodus rotundus shelters in the central region of São Paulo state, Brazil / Abrigos do morcego hematófago Desmodus rotundus na região central do estado de São Paulo, Brasil / Albergues de murciélagos hematophagus Desmodus rotundus em la región central del estado de São Paulo, Brasil

The vampire bat Desmodus rotundus is the main transmitter of domestic herbivorous rabies, which causes economic losses in cattle raising, being a serious public health problem. The use of Geographic Information Systems (GIS) allows the spatial monitoring of these bats' shelters, which is an efficient way to prevent and control rabies. This study located shelters of D. rotundus in the Eastern Center region of São Paulo state and identified the types of shelters used (natural or artificial), their proximity to water, sexual composition, and a population estimated of the colonies. Searches were carried out by colonies of vampire bats in natural and artificial shelters from 2002 to 2004, covering 18 municipalities. After being located, the shelters were georeferenced, being recorded the nature of the shelter (natural or artificial), use (shelters of males, maternities, digestive), and estimated population. The data were incorporated into a GIS, producing a thematic map of the shelter’s location and its distance from the local water bodies. There were found 1.567 D. rotundus distributed in 94 shelters, where only six shelters (6.38%) were natural (caves). Most shelters (79.78%) were sparsely populated, with up to 15 bats, including male shelters (46 shelters) and 30 maternities (63.82% of maternities). Five shelters (5.37%) had between 50 and 100 bats and only two shelters (2.15%) had a population greater than 100 bats. Sixs helters

O morcego hematófago Desmodus rotundus é o principal transmissor da raiva dos herbívoros domésticos, o qual causa prejuízos econômicos na pecuária, além de ser um sério problema de saúde pública. O uso de Sistemas de Informações Geográficas (SIG) permite o monitoramento espacial dos abrigos destes morcegos, sendo uma forma eficiente na prevenção e controle da raiva. Este estudo localizou abrigos de D. rotundus na região Centro Leste do estado de São Paulo e identificou os tipos de abrigos utilizados (naturais ou artificiais), sua proximidade a corpos de água, composição sexual e população estimada das colônias. Foram realizadas buscas por colônias de morcegos hematófagos em abrigos naturais e artificiais durante 2002 a 2004, abrangendo 18 municípios. Após serem localizados, os abrigos foram georreferenciados registrados quanto à natureza do abrigo (natural ou artificial), utilização (abrigos de machos, maternidades, digestório) e população estimada. Os dados foram incorporados em um SIG, produzindo-se um mapa temático da localização dos abrigos e sua distância dos corpos hídricos locais. Foram encontrados cerca de 1567 indivíduos da espécie D. rotundus distribuídos em 94 abrigos, onde apenas seis abrigos (6,38%) eram naturais (grutas). A maioria dos abrigos (79,78%) era pouco populoso, com até 15 morcegos, incluindo os abrigos de machos (46 abrigos) e 30 maternidades (63,82% das maternidades). Cinco abrigos (5,37%) possuíam entre 50 e 100 morcegos e apenas dois abrigos (2,15%) possuíam uma população superior a 100 morcegos. Seis abrigos...

El murciélago vampiro Desmodus rotundus es el principal transmisor de la rabia en los herbívoros domésticos, lo que provoca pérdidas económicas en el ganado, además de ser un grave problema de salud pública. El uso de Sistemas de Información Geográfica (SIG) permite el monitoreo espacial de los refugios de estos murciélagos, siendo una forma eficiente de prevenir y controlar la rabia. Este estudio localizó refugios de D. rotundus en la región Centro-Este del estado de São Paulo e identificó los tipos de refugios utilizados (naturales o artificiales), su proximidad a cuerpos de agua, composición sexual y población estimada de las colonias. Se realizaron búsquedas de colonias de murciélagos vampiros en refugios naturales y artificiales durante 2002 a 2004, abarcando 18 municipios. Una vez ubicados, se georreferenciaron los refugios y se registró la naturaleza del refugio (natural o artificial), uso (refugios masculinos, maternidades, digestivo) y población estimada. Los datos se incorporaron a un SIG, produciendo un mapa temático de la ubicación de los refugios y su distancia a los cuerpos de agua locales. Se encontraron 1567 individuos de la especie D. rotundus distribuidos en 94 refugios, donde solo seis refugios (6,38%) eran naturales (grutas). La mayoría delos refugios (79,78%) estaban escasamente poblados, con hasta 15 murciélagos, incluidos los refugios para machos (46 refugios) y 30 criaderos (63,82% de los criaderos). Cinco refugios (5,37%) tenían entre 50 y 100 murciélagos y solo dos refugios (2,15%) tenían una población superior a 100 murciélagos. Seis albergues...

Vampire bat Desmodus rotundus shelters in the central region of São Paulo state, Brazil / Abrigos do morcego hematófago Desmodus rotundus na região central do estado de São Paulo, Brasil / Albergues de murciélagos hematophagus Desmodus rotundus em la región central del estado de São Paulo, Brasil

The vampire bat Desmodus rotundus is the main transmitter of domestic herbivorous rabies, which causes economic losses in cattle raising, being a serious public health problem. The use of Geographic Information Systems (GIS) allows the spatial monitoring of these bats' shelters, which is an efficient way to prevent and control rabies. This study located shelters of D. rotundus in the Eastern Center region of São Paulo state and identified the types of shelters used (natural or artificial), their proximity to water, sexual composition, and a population estimated of the colonies. Searches were carried out by colonies of vampire bats in natural and artificial shelters from 2002 to 2004, covering 18 municipalities. After being located, the shelters were georeferenced, being recorded the nature of the shelter (natural or artificial), use (shelters of males, maternities, digestive), and estimated population. The data were incorporated into a GIS, producing a thematic map of the shelters location and its distance from the local water bodies. There were found 1.567 D. rotundus distributed in 94 shelters, where only six shelters (6.38%) were natural (caves). Most shelters (79.78%) were sparsely populated, with up to 15 bats, including male shelters (46 shelters) and 30 maternities (63.82% of maternities). Five shelters (5.37%) had between 50 and 100 bats and only two shelters (2.15%) had a population greater than 100 bats. Sixs helters…(AU)

O morcego hematófago Desmodus rotundus é o principal transmissor da raiva dos herbívoros domésticos, o qual causa prejuízos econômicos na pecuária, além de ser um sério problema de saúde pública. O uso de Sistemas de Informações Geográficas (SIG) permite o monitoramento espacial dos abrigos destes morcegos, sendo uma forma eficiente na prevenção e controle da raiva. Este estudo localizou abrigos de D. rotundus na região Centro Leste do estado de São Paulo e identificou os tipos de abrigos utilizados (naturais ou artificiais), sua proximidade a corpos de água, composição sexual e população estimada das colônias. Foram realizadas buscas por colônias de morcegos hematófagos em abrigos naturais e artificiais durante 2002 a 2004, abrangendo 18 municípios. Após serem localizados, os abrigos foram georreferenciados registrados quanto à natureza do abrigo (natural ou artificial), utilização (abrigos de machos, maternidades, digestório) e população estimada. Os dados foram incorporados em um SIG, produzindo-se um mapa temático da localização dos abrigos e sua distância dos corpos hídricos locais. Foram encontrados cerca de 1567 indivíduos da espécie D. rotundus distribuídos em 94 abrigos, onde apenas seis abrigos (6,38%) eram naturais (grutas). A maioria dos abrigos (79,78%) era pouco populoso, com até 15 morcegos, incluindo os abrigos de machos (46 abrigos) e 30 maternidades (63,82% das maternidades). Cinco abrigos (5,37%) possuíam entre 50 e 100 morcegos e apenas dois abrigos (2,15%) possuíam uma população superior a 100 morcegos. Seis abrigos...(AU)

El murciélago vampiro Desmodus rotundus es el principal transmisor de la rabia en los herbívoros domésticos, lo que provoca pérdidas económicas en el ganado, además de ser un grave problema de salud pública. El uso de Sistemas de Información Geográfica (SIG) permite el monitoreo espacial de los refugios de estos murciélagos, siendo una forma eficiente de prevenir y controlar la rabia. Este estudio localizó refugios de D. rotundus en la región Centro-Este del estado de São Paulo e identificó los tipos de refugios utilizados (naturales o artificiales), su proximidad a cuerpos de agua, composición sexual y población estimada de las colonias. Se realizaron búsquedas de colonias de murciélagos vampiros en refugios naturales y artificiales durante 2002 a 2004, abarcando 18 municipios. Una vez ubicados, se georreferenciaron los refugios y se registró la naturaleza del refugio (natural o artificial), uso (refugios masculinos, maternidades, digestivo) y población estimada. Los datos se incorporaron a un SIG, produciendo un mapa temático de la ubicación de los refugios y su distancia a los cuerpos de agua locales. Se encontraron 1567 individuos de la especie D. rotundus distribuidos en 94 refugios, donde solo seis refugios (6,38%) eran naturales (grutas). La mayoría delos refugios (79,78%) estaban escasamente poblados, con hasta 15 murciélagos, incluidos los refugios para machos (46 refugios) y 30 criaderos (63,82% de los criaderos). Cinco refugios (5,37%) tenían entre 50 y 100 murciélagos y solo dos refugios (2,15%) tenían una población superior a 100 murciélagos. Seis albergues...(AU)

Breeding biology of Long-tailed Cinclodes Cinclodes pabsti Sick, 1969 (Passeriformes: Furnariidae)

Abstract Cinclodes pabsti is an endemic passerine restricted to the highland areas in southern Brazil. The aim of this study was to provide information on its breeding biology. The nesting cavities along road cuts were monitored from May 2008 to March 2011. The survey was carried out monthly from May to July 2008, February to July 2009, 2010 (non-breeding season), weekly from August 2008 to January 2009, 2010 (breeding season), and on a 2 to 4-days basis from August 2010 to January 2011. The geographic location, physical characteristics, and soil/substrate type in which the nesting cavities were situated were recorded. The total number of cavities used in the three breeding seasons was 136, resulting in 295 nests. The distance of a nest to its nearest neighbor ranged from 24-2,368 m, with a higher number of nests (n = 34; 59.7%) in the distance interval of 24-500 m. There was a greater usage of cavities located in Inceptisols, and the distances of nesting cavity entrances to the ground and to the top of road cuts were 1.6 ± 0.9 m and 0.8 ± 0.62 m, respectively. The breeding season lasted 148 days from mid-August to early January. Clutch size (n = 256) varied from 2 to 3 eggs, and the eggs (n = 155) had a total length of 27.2 ± 1.3 mm, breadth of 20.9 ± 0.8 mm, and mass of 6.2 ± 0.7 g. The incubation phase lasted 17.3 ± 0.8 days and the nestling phase for 18.3 ± 1.5 days. The body mass of the chicks was 6.0 ± 1.0 g just after hatching and reached a maximum of 59.6 ± 2.4 g at 16 days of age. Our results can contribute to filling the gaps in knowledge of C. pabsti ecology, because its habitat is under high anthropic pressures and the information on its life history is yet limited.

Breeding biology of Long-tailed Cinclodes Cinclodes pabsti Sick, 1969 (Passeriformes: Furnariidae)

Abstract Cinclodes pabsti is an endemic passerine restricted to the highland areas in southern Brazil. The aim of this study was to provide information on its breeding biology. The nesting cavities along road cuts were monitored from May 2008 to March 2011. The survey was carried out monthly from May to July 2008, February to July 2009, 2010 (non-breeding season), weekly from August 2008 to January 2009, 2010 (breeding season), and on a 2 to 4-days basis from August 2010 to January 2011. The geographic location, physical characteristics, and soil/substrate type in which the nesting cavities were situated were recorded. The total number of cavities used in the three breeding seasons was 136, resulting in 295 nests. The distance of a nest to its nearest neighbor ranged from 24-2,368 m, with a higher number of nests (n = 34; 59.7%) in the distance interval of 24-500 m. There was a greater usage of cavities located in Inceptisols, and the distances of nesting cavity entrances to the ground and to the top of road cuts were 1.6 ± 0.9 m and 0.8 ± 0.62 m, respectively. The breeding season lasted 148 days from mid-August to early January. Clutch size (n = 256) varied from 2 to 3 eggs, and the eggs (n = 155) had a total length of 27.2 ± 1.3 mm, breadth of 20.9 ± 0.8 mm, and mass of 6.2 ± 0.7 g. The incubation phase lasted 17.3 ± 0.8 days and the nestling phase for 18.3 ± 1.5 days. The body mass of the chicks was 6.0 ± 1.0 g just after hatching and reached a maximum of 59.6 ± 2.4 g at 16 days of age. Our results can contribute to filling the gaps in knowledge of C. pabsti ecology, because its habitat is under high anthropic pressures and the information on its life history is yet limited.

Breeding biology of Long-tailed Cinclodes Cinclodes pabsti Sick, 1969 (Passeriformes: Furnariidae)

Cinclodes pabsti is an endemic passerine restricted to the highland areas in southern Brazil. The aim of this study was to provide information on its breeding biology. The nesting cavities along road cuts were monitored from May 2008 to March 2011. The survey was carried out monthly from May to July 2008, February to July 2009, 2010 (non-breeding season), weekly from August 2008 to January 2009, 2010 (breeding season), and on a 2 to 4-days basis from August 2010 to January 2011. The geographic location, physical characteristics, and soil/substrate type in which the nesting cavities were situated were recorded. The total number of cavities used in the three breeding seasons was 136, resulting in 295 nests. The distance of a nest to its nearest neighbor ranged from 24-2,368 m, with a higher number of nests (n = 34; 59.7%) in the distance interval of 24-500 m. There was a greater usage of cavities located in Inceptisols, and the distances of nesting cavity entrances to the ground and to the top of road cuts were 1.6 ± 0.9 m and 0.8 ± 0.62 m, respectively. The breeding season lasted 148 days from mid-August to early January. Clutch size (n = 256) varied from 2 to 3 eggs, and the eggs (n = 155) had a total length of 27.2 ± 1.3 mm, breadth of 20.9 ± 0.8 mm, and mass of 6.2 ± 0.7 g. The incubation phase lasted 17.3 ± 0.8 days and the nestling phase for 18.3 ± 1.5 days. The body mass of the chicks was 6.0 ± 1.0 g just after hatching and reached a maximum of 59.6 ± 2.4 g at 16 days of age. Our results can contribute to filling the gaps in knowledge of C. pabsti ecology, because its habitat is under high anthropic pressures and the information on its life history is yet limited.(AU)

Dissimilarity of parameterized maps generated in geostatistics and the assumption of an automatic software model in precision agriculture / Dissimilaridade de mapas gerados na geoestatística parametrizada e seu pressuposto modelo automático de software na agricultura de precisão

Geostatistics is the main technique used to efficiently determine spatial variability. The objective of this study was to evaluate the applicability of the principles of geostatistics in the use of semivariograms elaborated through parametric monitoring and the assumption automatically made by software in the map preparation of soil chemical attributes. Available phosphorus (P), potassium (K+), calcium (Ca²+), magnesium (Mg²+), base saturation (V%), sulfur (SO4²-), and pH were compared from the soil chemical attributes of 60 samples of a Typical Oxisol collected at a 0-20 cm depth and a distance of 300 m betweenthe points. The maps were compared using error matrices and evaluated by the Global Accuracy (GA), Kappa (K), and Tau (T) indexes. The parameterized semivariograms and the automatic software model assumption did not present a high coincidence for the available P and Mg²+, making it necessary to adjust the semivariogram variables in the spatial analysis as a function of the outliers, sum of squares of residuals, coefficient of determination, and cross-validation to better represent the variability of the data and thus avoid distortions of the sample point range that would affect the adequate representativeness of the attributes, which contrasts with the automatic model generated by the software.

A geoestatística é uma das principais técnicas utilizadas para determinar a variabilidade espacial dos atributos do solo. O objetivo deste trabalho foi comparar o uso de semivariogramas elaborados através do acompanhamento paramétrico da geoestatística e seu pressuposto modelo realizado automaticamente por software na confecção dos mapas de fertilidade do solo. Foram comparados os mapas dos valores de pH, fósforo (P), potássio (K+), cálcio (Ca²+), magnésio (Mg²+), enxofre (SO4²-) e saturação por bases (V%), provenientes de 60 amostras coletadas de área de Latossolo Vermelho Distrófico coletadas na profundidade 0-20 cm e malha de 300 m de distância entre os pontos. Os mapas foram comparados por matrizes de erro e avaliadas pelos índices Exatidão Global, Kappa e Tau. O uso de semivariogramas parametrizados e seu pressuposto modelo automático do software não apresentaram alta coincidência para os teores de P disponível e Mg²+, sendo necessário ajustes das variáveis dos semivariogramas na análise espacial em função dos outliers, soma dos quadrados dos resíduos, coeficiente de determinação e validação cruzada para melhor representação da variabilidade dos dados e assim, evitar distorções do alcance do ponto amostral para uma boa representatividade dos atributos estudados, o que contraria o modelo automático gerado pelo software.

Dissimilarity of parameterized maps generated in geostatistics and the assumption of an automatic software model in precision agriculture / Dissimilaridade de mapas gerados na geoestatística parametrizada e seu pressuposto modelo automático de software na agricultura de precisão

Geostatistics is the main technique used to efficiently determine spatial variability. The objective of this study was to evaluate the applicability of the principles of geostatistics in the use of semivariograms elaborated through parametric monitoring and the assumption automatically made by software in the map preparation of soil chemical attributes. Available phosphorus (P), potassium (K+), calcium (Ca²+), magnesium (Mg²+), base saturation (V%), sulfur (SO4²-), and pH were compared from the soil chemical attributes of 60 samples of a Typical Oxisol collected at a 0-20 cm depth and a distance of 300 m betweenthe points. The maps were compared using error matrices and evaluated by the Global Accuracy (GA), Kappa (K), and Tau (T) indexes. The parameterized semivariograms and the automatic software model assumption did not present a high coincidence for the available P and Mg²+, making it necessary to adjust the semivariogram variables in the spatial analysis as a function of the outliers, sum of squares of residuals, coefficient of determination, and cross-validation to better represent the variability of the data and thus avoid distortions of the sample point range that would affect the adequate representativeness of the attributes, which contrasts with the automatic model generated by the software.(AU)

A geoestatística é uma das principais técnicas utilizadas para determinar a variabilidade espacial dos atributos do solo. O objetivo deste trabalho foi comparar o uso de semivariogramas elaborados através do acompanhamento paramétrico da geoestatística e seu pressuposto modelo realizado automaticamente por software na confecção dos mapas de fertilidade do solo. Foram comparados os mapas dos valores de pH, fósforo (P), potássio (K+), cálcio (Ca²+), magnésio (Mg²+), enxofre (SO4²-) e saturação por bases (V%), provenientes de 60 amostras coletadas de área de Latossolo Vermelho Distrófico coletadas na profundidade 0-20 cm e malha de 300 m de distância entre os pontos. Os mapas foram comparados por matrizes de erro e avaliadas pelos índices Exatidão Global, Kappa e Tau. O uso de semivariogramas parametrizados e seu pressuposto modelo automático do software não apresentaram alta coincidência para os teores de P disponível e Mg²+, sendo necessário ajustes das variáveis dos semivariogramas na análise espacial em função dos outliers, soma dos quadrados dos resíduos, coeficiente de determinação e validação cruzada para melhor representação da variabilidade dos dados e assim, evitar distorções do alcance do ponto amostral para uma boa representatividade dos atributos estudados, o que contraria o modelo automático gerado pelo software.(AU)

Bat assemblages of protected areas in the state of Rio de Janeiro, Brazil

ABSTRACT We analyzed the bat assemblages found in protected areas in the state of Rio de Janeiro, Brazil, which is the best-sampled region of the Atlantic Forest. We selected 24 strict nature reserves and nine sustainable-use protected areas. We used data from inventories and complemented with data from the literature. We compared strict and sustainable-use protected areas, and tested whether the bat assemblages varied between habitat types. We tested the effect of geographic distance on the dissimilarity between bat assemblages, as well as the relationship between species composition and the size, mean altitude of the protected area, and capture effort. We compiled a total of 34,443 capture records, involving 67 species. Three species were captured only once, which raises cause for concern. Bat assemblages did not vary between protected area categories, but did vary among habitats with less than 1,000 captures. Assemblages were more similar to one another in geographically proximate areas. The size of the protected area and capture effort did not affect the composition of the bat assemblages, but altitude did influence this parameter. The Atlantic Forest is a priority biome for research and conservation, and reliable data on species distributions are essential for the development of conservation strategies.

RESUMO Analisamos as assembleias de morcegos encontradas em Unidades de Conservação (UCs) no estado do Rio de Janeiro, Brasil, que é a região melhor amostrada da Mata Atlântica. Selecionamos 24 UCs de Proteção Integral e nove de Uso Sustentável. Utilizamos dados de inventários e complementamos com dados da literatura. Comparamos as UCs de Proteção Integral e de Uso Sustentável e testamos se as assembleias de morcegos variavam entre os tipos de habitat. Testamos o efeito da distância geográfica na dissimilaridade entre as assembleias de morcegos, bem como a relação entre a composição das espécies e o tamanho, a altitude média das UCs e o esforço de captura. Compilamos um total de 34.443 registros de captura, de 67 espécies. Três espécies foram capturadas apenas uma vez, o que mostra motivos de preocupação. As assembleias de morcegos não variaram entre categorias das UCs, mas variaram entre habitats com menos de 1.000 capturas. As assembleias foram mais parecidas entre si em áreas geograficamente próximas. O tamanho da UC e o esforço de captura não afetaram a composição das assembleias de morcegos, mas a altitude influenciou esse parâmetro. A Mata Atlântica é um bioma prioritário para pesquisa e conservação, e dados confiáveis sobre a distribuição de espécies são essenciais para o desenvolvimento de estratégias de conservação.

Morphological and molecular characterization of Haemoproteus coatneyi and Haemoproteus erythrogravidus (Haemosporida: Haemoproteidae) in Passeriformes in Brazils Atlantic Forest / Caracterização morfológica e molecular de Haemoproteus coatneyi e Haemoproteus erythrogravidus (Haemosporida: Haemoproteidae) em Passeriformes da Mata Atlântica, Brasil

Haemoproteus spp. are protozoan parasites found in birds around the world. These parasites are identified through the morphology of gametocytes, phylogenetic analysis based on the mitochondrial cytb gene, and the parasites geographic distribution. The absence of erythrocytic merogony, high intraspecific genetic variation and low parasitemia in wild birds makes it essential to use integrative approaches that assist in the identification of these parasites. Thus, microscopic and molecular analyses, combined with spatial distribution, were carried out to verify the presence of Haemoproteus spp. in wild birds in Brazil. Light microscopy revealed one Tangara sayaca bird was parasitized by Haemoproteus coatneyi and, two specimens of Zonotrichia capensis presented Haemoproteus erythrogravidus. The morphology of the gametocytes of these two parasitic species showed high similarity. The molecular analysis revealed the presence of one lineage of H. coatneyi and two lineages of H. erythrogravidus, one of which is considered a new lineage. These lineages were grouped phylogenetically in separate clades, with low genetic divergence, and the H. erythrogravidus lineage emerged as an internal group of the lineages of H. coatneyi. The geographic distribution demonstrated that the two species occur in the American continent. This is the first report of H. erythrogravidus in Brazil.(AU)

Haemoproteus spp. são protozoários parasitos encontrados em aves de todo o mundo. A identificação desses parasitos é realizada por meio da morfologia dos gametócitos, da análise filogenética, baseada no gene mitoncodrial cytb e na distribuição geográfica do parasito. A ausência de merogonia eritrocítica, a alta variação genética intraespecífica e a baixa parasitemia em aves silvestres, tornam essencial a utilização de abordagens integrativas que auxiliem na identificação desses parasitos. Assim, análises microscópicas e moleculares, aliadas à distribuição espacial, foram realizadas para verificar a presença de Haemoproteus spp. em aves silvestres no Brasil. A microscopia óptica demonstrou que uma ave Tangara sayaca estava parasitada por Haemoproteus coatneyi, e dois espécimes de Zonotrichia capensis apresentavam Haemoproteus erythrogravidus, cujas morfologias dos gametócitos apresentaram alta similaridade. A análise molecular recuperou uma linhagem de H. coatneyi e duas linhagens de H. erythrogravidus, sendo uma dessas considerada nova linhagem. Essas linhagens se agruparam filogeneticamente em clados separados, apresentando baixa divergência genética, sendo que as linhagens de H. erythrogravidus emergiram como grupo interno às linhagens de H. coatneyi. A distribuição geográfica demonstrou que as duas espécies estão ocorrendo no continente americano. Este é o primeiro relato de H. erythrogravidus no Brasil.(AU)

A checklist of 'Psocoptera' (Psocodea) from Brazil: an update to the list of 2009 of García Aldrete and Mockford, with an identification key to the families

The described species of Psocoptera currently known for Brazil are listed, with state distribution and biogeographic status. An identification key to the families recorded in Brazil is presented.

A checklist of 'Psocoptera' (Psocodea) from Brazil: an update to the list of 2009 of García Aldrete and Mockford, with an identification key to the families

The described species of Psocoptera currently known for Brazil are listed, with state distribution and biogeographic status. An identification key to the families recorded in Brazil is presented.(AU)