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1.
Rev. bras. ciênc. avic ; 25(2): eRBCA-2022-1668, 2023. tab
Artigo em Inglês | VETINDEX | ID: biblio-1419081

Resumo

This study investigated the effect of resveratrol on the immune and inflammatory responses and the mRNA levels of splenic toll-like receptor (TLR)-4 signaling pathway-related genes of broilers under heat stress (HS). One hundred and sixty-two birds were allocated to three groups, each with 6 replicates, for 21 continuous days. The three treatments were as follows: the control group (22 ± 1 °C), the HS (33 ± 1 °C for 10 h d-1 and 22 ± 1 °C for the remaining time) group and the HS + resveratrol (400 mg kg-1) group. At the end of the trial, one bird per replicate close to the average body weight (BW) was selected, exsanguinated, and slaughtered. Compared with the control group, the HS treatment decreased (p<0.05) final BW, average daily gain (ADG), average daily feed intake (ADFI), relative weight of bursa of Fabricius and spleen, serum immunoglobulin (Ig) Y, IgA and interleukin (IL)-10 contents, and splenic IL-10 mRNA level, while it increased (p<0.05) feed/gain, mRNA levels of splenic tumor necrosis factor-α (TNF-α), TLR-4, nuclear factor-kappa-B (NF-κB), IL-1ß, and IL-6. Compared to the HS group, the HS+resveratrol group exhibited increased (p<0.05) final BW, ADG, relative weight of bursa of Fabricius and spleen, serum IgY, IgA and IL-10 contents, and splenic IL-10 mRNA level, while it exhibited lower (p<0.05) TNF-α, IL-1ß and IL-6 contents in serum, and splenic TLR4, TNF-α, IL-1ß, and NF-κB mRNA levels. In conclusion, resveratrol prevented a HS-impairment of the immune function of broilers by blocking the abnormal activation of the TLR4 signaling pathway.(AU)


Assuntos
Animais , Galinhas/imunologia , Resposta ao Choque Térmico/imunologia , Resveratrol/efeitos adversos , Receptor 4 Toll-Like/análise
2.
Braz. j. vet. pathol ; 16(1): 54-59, mar. 2023. ilus
Artigo em Inglês | VETINDEX | ID: biblio-1425378

Resumo

Histiocytic sarcoma (HS) is uncommon malignant neoplasia of round cells with marked predilection in Rottweiler and Bernese Mountain. The disseminated form, which mainly affects the spleen, lungs, lymph nodes, bone marrow, skin, and subcutis, presents a quick and aggressive clinical behavior. Hemangiosarcoma (HAS) is a malignant neoplasm of endothelial vessel cells commonly reported to affect the right atrium of dogs' hearts. A male Rottweiler, five years old, presented flaccid paraplegia and progressive muscular atrophy in the temporal, masseter, and limbs muscles; Due to the clinical stage of the animal, euthanasia was conducted. During the necroscopic examination, it was noticed that several masses presented different sizes; some were whitish, and others were reddish and spread in multiple organs (lungs, heart, spleen, stomach, kidneys, brain, medulla, skeletal muscle, and pre-scapular lymph node). Microscopically, in some organs such as the stomach, right ventricle, lungs, and medulla, it was noticed a proliferation of myeloid cells, highly cellular, with poor demarcation, no encapsulation, and with the infiltrative growth pattern of cells with high pleomorphism. Numerous tumoral emboli were observed in the spleen, brain, skeletal muscle, and lymph node. These cells were submitted for immunohistochemistry and were positive for CD18 (HS antibody). In the right atrium, liver, and kidney it was observed malignant and infiltrative endothelial proliferation (HSA) and emboli in the medulla. Therefore, we conclude that both neoplasms (HS and HSA) cause the animal's paraplegia due to their embolism and metastasis to the spinal cord and skeletal muscle.(AU)


Assuntos
Animais , Doenças do Cão , Sarcoma Histiocítico/veterinária , Hemangiossarcoma/diagnóstico , Cães
3.
Rev. bras. zootec ; 52: e20210040, 2023. tab
Artigo em Inglês | VETINDEX | ID: biblio-1436783

Resumo

In this study, we aimed to determine the effect of Moringa oleifera essential oil (MOEO) supplementation to rations of Japanese quail ( Coturnix coturnix japonica ) grown under heat stress (HS) on some adipokine (visfatin, adiponectin, and chemerin), intestinal (citrulline), 3,5,3'-triiodothyronine (T3), thyroxine (T4), and thyroid-stimulation hormone (TSH) levels in the serum. For this purpose, 320 day-old male quail chicks were used. The study was conducted over 42 days, including a 7-d adaptation period and a 35-d experimental period. Eight trial groups were established, each including 10 quail: CT (control temperature, 25 ℃), MOEO 200, MOEO 400, MOEO 600, HSC (HS control), SMOEO 200, SMOEO 400, and SMOEO 600. Based on the results, a dose-dependent decrease was observed on days 21 and 42 in serum adiponectin and T3 in the stress and stress-free groups compared with the control group due to MOEO supplementation. The lowest decrease was observed in the MOEO 600 mg/kg dose group. In addition, an increase in stressed groups was observed when examining serum citrulline levels, while chemerin levels did not have a statistically significant effect. While the decrease in serum visfatin, T4, and TSH levels on the 21st day did not have a statistically significant effect, a significant effect was determined on the 42nd day. The addition of MOEO at 600 mg/kg to the ration may be beneficial in preventing intestinal damage and inflammation that may occur due to HS.


Assuntos
Animais , Hormônios Tireóideos , Óleos Voláteis , Transtornos de Estresse por Calor , Coturnix , Moringa oleifera , Dieta/veterinária
4.
Acta cir. bras ; 38: e382923, 2023. tab, graf, ilus
Artigo em Inglês | VETINDEX | ID: biblio-1505463

Resumo

Purpose: To explore effect and mechanism of olsalazine of Chinese generic drugs on ulcerative colitis induced by dextran sulfate sodium salt (DSS) in BALB/c mice. Methods: The mouse model of ulcerative colitis was induced by free drinking of 3% (w/v) DSS aqueous solution for seven days. The mice were treated with olsalazine (0.6 g·kg-1) of Chinese generic drugs. The therapeutic effect of olsalazine on ulcerative colitis mice was evaluated by measuring disease activity index (DAI), colonic mucosal injury index (CMDI), histopathological score (HS), and detected the expression levels of interleukin (IL)-2, IL-10, tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), IL-1ß in serum and IL-7, IL-17, IL-22, epidermal growth factor (EGF), transforming growth factor ß1 (TGF-ß1) in colonic homogenate of mice. Results: Olsalazine significantly increased the contents of IL-2, IL-10, IL-22, TGF and EGF in ulcerative colitis rats, and significantly decreased the scores of DAI, CMDI, HS and the contents in IL-7, IL-17, TNF-α, IL-1ß and IFN-γ when compared with the model group. It improved the degree of colonic lesion in ulcerative colitis mice. Conclusions: It was suggested that olsalazine has a therapeutic effect on ulcerative colitis induced by DSS in mice, and the mechanism may be related to the increase of IL-2, IL-10, IL-22, TGF, and EGF and the decrease of the expression of IL-7, IL-17, TNF-α, IL-1ß, and IFN-γ.


Assuntos
Animais , Camundongos , Colite Ulcerativa/tratamento farmacológico , Sulfato de Dextrana , Medicamentos Genéricos
5.
J. Anim. Behav. Biometeorol ; 11(1): e2023002, Jan. 2023. ilus, graf
Artigo em Inglês | VETINDEX | ID: biblio-1434674

Resumo

The four fish species, Trichogaster pectoralis, Barbodes schwanenfeldii, Osphronemus gouramy, and Wallago leeri have the same body size, representing different habitats. Therefore, this study aimed to determine the four species swimming abilities and speeds, including sustained, maximum sustained, prolonged swimming speed, and burst speed. The four species were brought using oxygenated plastic bags to the Aquatic Resources Utilization Laboratory at Riau University. Then it was acclimated in an aquarium measuring 100×50×50 cm with an aeration and filtering system to maintain water quality. The four groups of species were forced to swim in the swimming channel of the flume tank, with different current speeds for each individual tested. The swimming activity of the fish was recorded using a high-speed video camera (Casio HS Exlim, EX-ZR400, Casio Computer Co. LTD, Japan). The results showed that the swimming speed of B. schwanenfeldii was higher than O. gouramy, T. pectoralis, and W. leeri. The maximum sustained swimming speed of B. schwanenfeldii is about 5.1 BL/s, and 14.2 BL/s for the burst speed. Meanwhile, the sustained swimming speed of O. gouramy (1.6 BL/s) and the burst speed is 7.2 BL/s. The maximum sustained swimming speed of T. pectoralis was 1.6 BL/s, and the burst speed was 5.7 BL/s. In addition, the maximum sustained swimming speed of W. leeri is only 0.5 BL/s and 2.4 BL/s for the burst speed. The ability of these four fish to swim was influenced by the morphology of the tail fin, body shape, and the habitat where they live.


Assuntos
Animais , Comportamento Animal , Fauna Aquática , Peixes/fisiologia , Locomoção
6.
Arq. bras. med. vet. zootec. (Online) ; 75(4): 721-734, July-Aug. 2023. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1447346

Resumo

The objective of this work was to compare the dry matter intake, milk yield and quality, physiological and biochemical parameters in Holstein (n=10) and Jersey (n=10) cows under heat stress and insolation, in two treatments: CL - cooling by ventilation and sprinkling and HS - heat stress and insolation. Data were submitted to ANOVA. There was an interaction between treatment and breed and day effect for dry matter intake. For consumption in % of body weight, CL and Jersey cows consumed more. CL cows produced more milk and 3.5% fat-corrected milk. Feed efficiency was similar between treatments and breeds. Fat, lactose, total solids, and somatic cell score did not differ. The concentration of milk urea nitrogen was higher for CL cows. Milk from Holstein cows had greater stability to alcohol, and from HT cows had a greater freezing point of milk. HT cows had higher respiratory rates in the morning and surface temperatures in the afternoon. There were no differences in beta-hydroxybutyrate and glucose concentrations. Heat stress, with insulation, reduces intake, especially in Holstein cows, as well as milk production and increases the freezing point of milk, respiratory rate, and surface temperature.


O objetivo deste trabalho foi comparar o consumo de matéria seca, a produção e a qualidade do leite, os parâmetros fisiológicos e bioquímicos em vacas das raças Holandesa (n=10) e Jersey (n=10) sob estresse calórico e insolação, em dois tratamentos: CL - resfriamento por ventilação e aspersão; HS - estresse térmico e insolação. Os dados foram submetidos à análise de variância. Houve interação entre tratamento e raça e efeito de dia para consumo de matéria seca. Para consumo em % de peso vivo, vacas CL e Jersey consumiram mais. Vacas CL produziram mais leite e leite corrigido a 3,5% de gordura. A eficiência alimentar foi similar entre tratamentos e raças. Teores de gordura, lactose, sólidos totais e escore de células somáticas não diferiram. A concentração de nitrogênio ureico do leite foi maior para vacas CL. O leite das vacas Holandesas apresentou maior estabilidade ao álcool, e de vacas HT maior crioscopia. Vacas HT apresentaram maior frequência respiratória de manhã e temperatura superficial à tarde. Não houve diferenças para concentração de beta-hidroxibutirato e glicose. O estresse calórico, com insolação, reduz o consumo, especialmente em vacas Holandesas, bem como a produção de leite, com aumento da crioscopia, elevando a frequência respiratória e a temperatura superficial.


Assuntos
Animais , Bovinos , Insolação , Radiação Solar , Leite/química , Temperatura Alta/efeitos adversos
7.
Ciênc. rural (Online) ; 53(6): 1-8, 2023. tab
Artigo em Inglês | VETINDEX | ID: biblio-1413095

Resumo

Sapajus libidinosus is a New World primate belonging to the Cebidae family that lives in the caatinga and cerrado, which are known Brazilian biomes. It is currently classified as near threatened, almost endangered, by the main animal protection organizations. Knowledge of biochemistry, the C-reactive protein, and the proteinogram are important for the preservation of this species. Our study established reference intervals for several biochemical variables and the proteinogram. For this purpose, blood samples were collected from 50 S. libidinosus monkeys (25 males and 25 females) kept in captivity at the Brazilian state of Paraíba. Descriptive analysis and reference intervals were estimated following the guidelines of the American Society of Veterinary Clinical Pathology, using the Reference Value Advisor 2.1 software. In the overall population (n=50), significant differences (P < 0.05) were noted for creatinine and total proteins when considering the age factor (higher in adults), as well as for albumin and indirect bilirubin (higher in juveniles). Sex-associated differences (females versus males, P < 0.05) were reported for serum urea and creatinine (higher in males), as well as for albumin and Beta-Globulins (higher in females). In conclusion, to the authors' best knowledge, the present results are innovative and can be used as reference intervals for assessing the animals' health status. Moreover, it is also pioneer in determining the C-reactive protein in this species. It is important to emphasize that gender and age categories can have an influence and should be considered when interpreting the tests results.


O Sapajus libidinosus é um primata do novo mundo pertencente à família Cebidae que habita os biomas da caatinga e cerrado do Brasil. Atualmente esta classificado como quase ameaçado pelos principais orgãos de proteção animal. O conhecimento da bioquímica sérica, proteína C reativa e proteinograma são importantes na preservação desta espécie. O nosso estudo tem como objetivo determinar os intervalos de referência para várias variáveis bioquímicas e proteinograma. Para isso, foram colhidas amostras de sangue de 50 macacos da espécie S. libidinosus (25 machos e 25 fêmeas) mantidos em cativeiro no estado da Paraíba, Brasil. A análise descritiva e os cálculos dos intervalos de referência foram estimados segundo as diretrizes da Sociedade Americana de Patologia Clínica Veterinária com o uso do software Reference Value Advisor 2.1. Na população total (n=50), considerando o factor idade, foram observadas diferenças significativas (P < 0,05) para a creatinina e proteínas totais (valores superiores nos adultos) e para a albumina e bilirrubina indirecta (valores superiores nos jovens). Foram encontradas diferenças associadas ao sexo (fêmeas versus machos, P < 0,05) para a ureia e creatinina séricas (valores superiores nos machos) e albumina e Beta-Globulinas (valores superiores nas fêmeas). Conclui-se que, tanto quanto é do conhecimento dos autores, os resultados deste estudo são inovadores e podem ser utilizados como intervalos de referência para avaliação do estado de higidez dos animais e é pioneiro na determinação da proteína C reativa nesta espécie. É importante salientar que o sexo e a faixa etária podem ter influência e devem ser levados em consideração pelos profissionais na interpretação dos exames.


Assuntos
Animais , Bioquímica , Proteína C-Reativa , Biomarcadores , Cebus , Sapajus
8.
Anim. Reprod. (Online) ; 20(1): e20220086, 2023. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1418538

Resumo

The identification of putative prognostic factors in canine mammary neoplasms (CMNs) has been focused on tissue-specific biomarkers, but the serum biomarkers, including cancer antigen 15-3 (CA 15-3), c-reactive protein (CRP), and lactate dehydrogenase (LDH) have been demonstrated to display clinical application in cases of CMNs. The aim of the study was to evaluate the levels of these serum biomarkers and their association with well-established prognostic factors in CMNs. Samples from 15 female canines with CMNs and 15 clinically healthy ones were collected. The results were evaluated using the Tukey's, Pearson, or Spearman tests. The cut-off point, sensitivity, specificity, and area under curve (AUC) were evaluated using the receiver operating characteristic (ROC) curve analysis in a logistic regression model (P<0.05). The levels of CA 15-3, CRP and LDH were significantly higher in the serum of female dogs with CMNs compared to the healthy ones. Moreover, these factors were positively correlated with ulceration, tumor size, histopathological grade, metastatic lymph node, and clinical staging. Female dogs with CMNs were found to exhibit highest serum levels of CA 15-3, CRP, and LDH. Therefore, they can be applied to improve the efficacy of the diagnosis and prognostic evaluation in casas of CMNs.(AU)


Assuntos
Animais , Feminino , Neoplasias Mamárias Animais/diagnóstico , Cães , Glândulas Mamárias Animais/anatomia & histologia , Proteína C-Reativa/efeitos adversos , Biomarcadores , Mucina-1/efeitos adversos , L-Lactato Desidrogenase/efeitos adversos
9.
Rev. bras. ciênc. avic ; 24(3): eRBCA-2021-1450, 2022. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1416142

Resumo

The objective of the study was to evaluate the productive, carcass, and tibia mineralization responses in broiler chickens supplemented with a liquid extract of humic substances (HS) in the drinking water. Chicks were housed in holding cages from 8-42 days of age and were randomly assigned to one of five increasing HS levels in the drinking water (0, 161, 322, 483, and 644 µg/L). At 21 and 42 days, to obtain carcass and tibia measurements half of the broilers were slaughtered. ANOVA and linear regression were used to analyze the data. The HS chemical composition and flat structures were estimated. At 21 days, increasing levels of HS in the drinking water resulted in a cubic response on breast weight (p<0.05), tibia ashes percentage (p<0.05) and tibia Ca percentage, as well as a linear increasing response (p<0.05) on P percentage. HS elicited a quadratic response on the tibia DM percentage (p<0.05), Ca content (p<0.01), and P content (p<0.05) at 42 days. The optimal HS supplementation level to achieve the highest tibia DM percentage, Ca and P content were 345.00, 322.46, and 347.75 µg/L, respectively. Increasing HS levels also resulted in a cubic response in tibia Ca (p<0.05) and P percentage (p<0.01). In conclusions, HS supplementation in drinking water improved bone mineralization in broiler chickens at 21 and 42 days of age.(AU)


Assuntos
Animais , Calcificação Fisiológica/fisiologia , Galinhas/fisiologia , Substâncias Húmicas/análise , Tíbia/fisiologia , Suplementos Nutricionais/análise
10.
Ciênc. anim. bras. (Impr.) ; 23: e71852E, 2022. graf
Artigo em Inglês | VETINDEX | ID: biblio-1384502

Resumo

The objective of this study was to evaluate the influence of thermal shock on oocytes used in the production of in vitro embryos (IVP) of high productivity Holstein cows on the day of follicular aspiration (OPU; 0), 30, 60 and 90 days before the OPU. From the mean temperature on day 0 and on the previous 30, 60 and 90 days, they were classifed into comfort group (TC; up to 15°C) and heat stress (HS; above 15°C) groups.Anegative influence was observed on oocytes and viable embryos (total and grade I). The heat stress in the periods of 30 and 60 days prior to OPU resulted in lower production of viable oocytes (P=0.0028; P=0.0092, respectively). Under stress, on the day of OPU (HS-OPU), cows showed no reduction in the amount of viable oocytes (P=0.5497) and there was no influence of temperature for the group stressed 90 days before OPU (P=0.8287). For total embryos, the difference occurred only in the HS-30 group (P=0.0317), where the groups HS-OPU, HS-60, HS-90 presented, respectively, P=0. 1987, P=0.0596 and P=0.4580. Regarding the production of embryos of grade 1, there was no difference for the groups HS-OPU (P=0.2291) and HS-90 (P=0.2868), but there was a reduction for HS-30 (P=0.0143) and HS-60 (P=0.0253). In summary, heat stress had a negative impact when it occurred 30 or 60 days before follicular aspiration. In addition, 30 days seems to be the period of more susceptibility and that causes the greatest deleterious effects on oocyte viability and IVP.(AU)


Objetivou-se avaliar a infuência do estresse térmico em oócitos utilizados na produção in vitro de embriões (PIV) bovinos da raça Holandesa de alta produtividade no dia da aspiração folicular (OPU; 0), 30, 60 e 90 dias antes da OPU. A partir da temperatura média no dia 0 e aos 30, 60 e 90 dias anteriores, foram classificados nos grupos conforto (CT; até 15°C) e estresse por calor (ET -acima de 15°C). Observou-se infuência negativa em oócitos e embriões viáveis (total e grau I). A submissão ao estresse térmico nos períodos de 30 e 60 dias anteriores à OPU resultou em menor produção de oócitos viáveis (P=0,0028; P=0,0092, respectivamente). Sob estresse, no dia da OPU (ET-OPU), as vacas não apresentaram redução na quantidade de oócitos viáveis (P=0,5497) e não houve infuência da temperatura para o grupo estressado 90 dias antes da OPU (P=0,8287). Para embriões totais, a diferença ocorreu apenas no grupo ET-30 (P=0,0317), onde os grupos ET-OPU, ET-60, ET-90 apresentaram, respectivamente, P=0,1987, P=0,0596 e P=0,4580. Em relação à produção de embriões grau 1, não houve diferença para os grupos ET-OPU (P=0,2291) e ET-90 (P=0,2868), porém houve redução para ET-30 (P=0,0143) e ET- 60 (P=0,0253). Em resumo, o estresse por calor teve impacto negativo quando ocorreu 30 ou 60 dias antes da aspiração folicular. Além disso, 30 dias parece ser o período de maior suscetibilidade e que causa os maiores efeitos deletérios na viabilidade oocitária e na PIV.(AU)


Assuntos
Animais , Oócitos , Reprodução , Bovinos/crescimento & desenvolvimento , Transtornos de Estresse por Calor , Estruturas Embrionárias
11.
J. Anim. Behav. Biometeorol ; 10(1): 2209, jan. 2022. tab, ilus
Artigo em Inglês | VETINDEX | ID: biblio-1438211

Resumo

The problem of free radical pathologies in high temperatures in animal husbandry and veterinary medicine is extremely important scientific and practical. In the process of hyperthermia, the structure of cell membranes, the permeability of membranes, and membrane transport systems change, which cause the oxidation of unsaturated fatty acids and the accumulation of toxic products of a radical nature. This plays an important role in the development of many pathological conditions. The review article describes the problem of the influence of heat stress (HS) on a living organism and the problem of hyperthermia in animal husbandry. Emphasis is placed on the role of the body's antioxidant system in a state of HS and the use of antioxidant drugs as additives into feed for farm animals, aimed at mitigating the course and harmful effects in HS. On the one hand, our goal was to describe in an accessible form the main complex biochemical disorders in the body under HS conditions, which lead to a deterioration in the welfare and loss of productivity of animals. On the other hand, we tried to show the special role of the antioxidant defense system in preventing the development of oxidative stress in its classical representation. Also, we substantiated the choice in favor of using natural and synthetic antioxidants as one of the strategies for the prevention of HS in modern animal husbandry.(AU)


Assuntos
Animais , Feminino , Bovinos , Estresse Fisiológico , Bovinos/fisiologia , Reações Bioquímicas , Antioxidantes/fisiologia
12.
Ciênc. rural (Online) ; 52(8): 20210040, 2022. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1360343

Resumo

Varronia curassavica is a medicinal species native to Brazil; its anti-inflammatory properties are attributed to α-humulene and ß-caryophyllene. This study to investigated the population variability of V. curassavica. Leaves were collected from 40 individuals of a natural population and transferred to glass flasks (2 mL). The volatiles were extracted and analyzed using static headspace gas chromatography coupled with mass spectrometry (HS-GC-MS). A total of 17 compounds were detected: monoterpenes (1.6% - 68%) and sesquiterpenes (25.7% - 79.0%), including α-humulene and ß-caryophyllene. Multivariate analysis was performed to estimate the dissimilarity of individuals separated the compounds into four groups. Group I was characterized by the content of α-pinene, groups II and IV by ß-caryophyllene, and group III by ß-pinene. The abundance of α-humulene showed small variations; while ß-caryophyllene showed larger variations that contributed to the population variability. This is the first report on the chemical composition analysis of volatile compounds in V. curassavica using the HS-GC-MS technique.


Varronia curassavica é uma espécie medicinal nativa no Brasil e sua propriedade anti-inflamatória é atribuída aos compostos α-humuleno e ß-cariofileno. O objetivo foi estudar a variabilidade de população de V. curassavica. Foram coletadas folhas de 40 indivíduos de população natural, sendo estas transferidas para frascos de vidro (2 mL). Os voláteis foram extraídos e analisados por headspace estático, associado à cromatografia gasosa, acoplada à espectrometria de massas (HS-CG-EM). Detectou-se 17 compostos: monoterpenos (1,6% - 68%) e sesquiterpenos (25,7% - 79,0%), incluindo o α-humuleno e o ß-cariofileno. A análise multivariada foi realizada para estimar a dissimilaridade dos indivíduos, a qual separou quatro grupos. O grupo I foi caracterizado pela presença do α-pineno, os grupos II e IV do ß-cariofileno e o grupo III do ß-pineno. O teor do α-humuleno apresentou pequenas variações, enquanto o do ß-cariofileno contribuiu mais para a variabilidade da população. É o primeiro relato de análise da composição química de compostos voláteis de V. curassavica utilizando a técnica HS-GC-MS.


Assuntos
Boraginaceae/genética , Boraginaceae/química , Cromatografia Gasosa-Espectrometria de Massas
13.
Rev. bras. zootec ; 51: e20210154, 2022. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1442849

Resumo

The objective of this study was to evaluate the effects of dietary supplementation of purified lignin on the performance, relative organ weights, serum metabolites, and gene expression profiles of broiler chickens subjected to cyclic heat stress (HS). At 22 days old, 280 broilers were distributed in a completely randomized design with four treatments, ten repetitions, and seven birds per experimental unit. The birds were subjected to daily cyclic HS. A high temperature of 32±1 °C was maintained for 10 h/day (08:00­18:00 h), while a temperature of 22±1 °C was maintained for the remaining time. Treatments were a basal diet or basal diet with the addition of 5, 10, or 15 g of purified lignin/kg of diet. Data were analyzed using one-way ANOVA and means were compared by Tukey's test at 0.05 significance. There was no effect of lignin supplementation on performance, carcass yield, relative weights of the bursa, spleen, and liver, or serum levels of glucose, triglycerides, uric acid, malondialdehyde, triiodothyronine, or tetraiodothyronine. The abundance of mRNA of heat shock protein 70, nuclear factor-κB, glutathione peroxidase, and Cu,Zn-superoxide dismutase in the liver was similarly unaffected by treatments. Purified lignin supplementation does not improve performance or the antioxidant response of broiler chickens subjected to HS.(AU)


Assuntos
Animais , Galinhas/fisiologia , Lignina/efeitos adversos , Antioxidantes/efeitos adversos , Transtornos de Estresse por Calor/veterinária
14.
Rev. bras. reprod. anim ; 46(2): 148-153, Abril-Junho 2022.
Artigo em Português | VETINDEX | ID: biblio-1378119

Resumo

Os testículos, são mantidos no escroto a uma temperatura ~3-5°C abaixo da corporal. Quando a temperatura das gônadas se eleva, instala-se um quadro de estresse térmico (ET) testicular. O ET afeta a espermatogênese, e observam-se, já na primeira semana pós-ET, impactos na cinética, concentração e morfologia espermática. Classicamente, tais efeitos eram creditados à incapacidade da circulação local de atender ao aumento do metabolismo testicular devido ao aumento da temperatura local. Contudo, estudos recentes demonstraram que a hipóxia não era a causa da degeneração testicular. Atualmente, credita-se os efeitos deletérios do ET ao aumento local das espécies reativas de O2. Nesta situação, apesar da ativação de mecanismos antioxidantes (aumento das HSP e GPX1) e de proteção do DNA (aumento da P53), estes não são suficientes, sendo desencadeada a apoptose. Os efeitos deletérios do ET testicular podem ser mitigados pela melatonina, que pode ser tanto administrada aos animais ou adicionada ao sêmen para que desencadeie seus efeitos protetores.(AU)


The testes are kept in the scrotum at a ~3-5°C below body core temperature. When the temperature of the gonads increases, a process called heat stress (HS) takes place. The HS impairs spermatogenesis, and in the first week post-HS, impacts in sperm kinetics, concentration, and morphology are observed. Classically, such effects were credited to the incapacity of the local circulation to sustain the higher testicular metabolism due to the increased temperature. However, recent studies demonstrated that it was not the cause of testicular degeneration. The novel perspective credits the deleterious impacts of the HS to the local increase of the reactive oxygen species. Importantly, although there's an activation of antioxidant defenses (increase in HSP and GPX1) and DNA protection (increase in P53), such mechanisms are not sufficient, unfolding the apoptotic cascade. Lastly, some of the negative effects of HS can be mitigated by melatonin, which can either be given to the animals or added to the sperm to exert its protective effects.(AU)


Assuntos
Animais , Masculino , Bovinos , Testículo/fisiopatologia , Resposta ao Choque Térmico/fisiologia , Hipóxia/veterinária , Espermatogênese/fisiologia , Regulação da Temperatura Corporal , Espécies Reativas de Oxigênio/efeitos adversos , Apoptose
15.
J. Anim. Behav. Biometeorol ; 10(1): 1-8, jan. 2022. tab, ilus
Artigo em Inglês | VETINDEX | ID: biblio-1484122

Resumo

The problem of free radical pathologies in high temperatures in animal husbandry and veterinary medicine is extremely important scientific and practical. In the process of hyperthermia, the structure of cell membranes, the permeability of membranes, and membrane transport systems change, which cause the oxidation of unsaturated fatty acids and the accumulation of toxic products of a radical nature. This plays an important role in the development of many pathological conditions. The review article describes the problem of the influence of heat stress (HS) on a living organism and the problem of hyperthermia in animal husbandry. Emphasis is placed on the role of the body's antioxidant system in a state of HS and the use of antioxidant drugs as additives into feed for farm animals, aimed at mitigating the course and harmful effects in HS. On the one hand, our goal was to describe in an accessible form the main complex biochemical disorders in the body under HS conditions, which lead to a deterioration in the welfare and loss of productivity of animals. On the other hand, we tried to show the special role of the antioxidant defense system in preventing the development of oxidative stress in its classical representation. Also, we substantiated the choice in favor of using natural and synthetic antioxidants as one of the strategies for the prevention of HS in modern animal husbandry.


Assuntos
Feminino , Animais , Bovinos , Bovinos , Estresse Oxidativo/efeitos dos fármacos , Fenômenos Bioquímicos/fisiologia , Resposta ao Choque Térmico
16.
Semina ciênc. agrar ; 43(2): 693-712, mar.-abr. 2022. graf, tab
Artigo em Inglês | VETINDEX | ID: biblio-1368892

Resumo

The interplay between biochemical characteristics and the generation of volatile compounds in 11 type II sourdough fermented by single strains of lactic acid bacteria (LAB) was studied. Samples were collected at 0, 6, 9, 12, 15, 18 and 24h for analyses of microbial growth, pH, titratable acidity and CO2 production. During the first 12h, the LABs entered the stationary phase, and the formation of organic and carboxyl acids, alcohols, and esters were observed. Although acidity is an important characteristic of sourdough, in this work increasing the acetic acid content decreased yeast growth and the CO2 retention capacity of the doughs. The main carbohydrate consumed by autochthonous yeast was influenced by the LAB added (homo-or heterofermentative), as observed by correlation analysis. Maltose and glucose showed a strong and negative correlation with the yeast cell density in the dough fermented by homo and heterofermentative LAB, respectively. Moreover, LAB had an important effect on the aromatic profile, being the alcohols, aldehydes, alkanes, organics acids and esters mainly groups characterized. Altogether, 100 different volatile compounds were identified; however, each dough had a different volatile profile. This study shows, for the first time, the influence of a single strain of LAB on the characteristics of type II sourdough.(AU)


As características bioquímicas e a produção de compostos voláteis em 11 diferentes sourdough tipo II produzido com uma única cepa de bactérias do ácido láctico (BAL) em foi estudada. As amostras foram coletadas às 0, 6, 9, 12, 15, 18 e 24 h para análises de crescimento microbiano, pH, acidez titulável e produção de CO2. Durante as primeiras 12 h, as BAL entraram em fase estacionária, sendo observada a formação de ácidos orgânicos e carboxílicos, álcoois e ésteres. Embora a acidez seja uma característica importante do sourdough, neste trabalho o aumento do teor de ácido acético diminuiu o crescimento das leveduras e a capacidade de retenção de CO2 nas massas. Também foi observado que o principal carboidrato consumido pelas leveduras autóctones foi influenciado pela BAL adicionada (homo ou heterofermentativas), conforme observado pela análise de correlação. A maltose e a glicose apresentaram uma correlação forte e negativa com a densidade celular de levedura na massa fermentada por BAL homo e heterofermentativas, respectivamente. Além disso, a BAL teve efeito importante no perfil aromático, sendo os álcoois, aldeídos, alcanos, ácidos orgânicos e ésteres os principais compostos caracterizados. Ao todo, foram identificados 100 compostos voláteis diferentes; no entanto, cada massa apresentou um perfil volátil diferente. Este estudo mostra, pela primeira vez, a influência de uma única cepa de BAL nas características de sourdough tipo II.(AU)


Assuntos
Reações Bioquímicas , Ácido Láctico , Limosilactobacillus reuteri/química , Fenômenos Microbiológicos , Compostos Orgânicos Voláteis , Lactobacillales
17.
Acta cir. bras ; 37(5): e370505, 2022. tab, graf, ilus
Artigo em Inglês | VETINDEX | ID: biblio-1393756

Resumo

Purpose: To investigate the effects of Periplaneta americana L. on ulcerative colitis (UC) induced by a combination of chronic stress (CS) and 2,4,6-trinitrobenzene sulfonic acid enema (TNBS) in rats. Methods: The experiment UC model with CS was established in rats by a combination of chronic restraint stress, excess failure, improper, and TNBS. The body weight, disease activity index (DAI), colonic mucosal injury index (CMDI), histopathological score (HS) and pro-inflammatory mediators were measured. The content of corticotropin-releasing hormone (CRH) in hypothalamus or adrenocorticotropic hormone (ACTH) and corticosteroids (CORT) in plasma were evaluated by enzyme-linked immunosorbent assay. The proportion of T lymphocyte subsets was detected by flow cytometry, and gut microbiota was detected by 16S rDNA amplicon sequencing. Results: Weight loss, DAI, CMDI, HS and proinflammatory mediators were reversed in rats by P. americana L. treatment after UC with CS. Increased epidermal growth factor (EGF) was observed in P. americana L. groups. In addition, P. americana L. could reduce the content of CRH and ACTH and regulate the ratio of CD3+, CD3+CD8+ and CD3+CD4+CD25+/CD4+ in spleen. Comparably, P. americana L. changes composition of gut microbiota. Conclusions: The ethanol extract of Periplaneta Americana L. improves UC induced by a combination of CS and TNBS in rats.


Assuntos
Animais , Ratos , Periplaneta , Terapêutica , Colite Ulcerativa , Etanol , Microbioma Gastrointestinal
18.
Colloq. Agrar ; 18(2): 33-41, mar.-abr. 2022. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1399106

Resumo

Plant species Phytolacca americana (Phytolaccaceae) can be classified as UFP (Unconventional Food Plant); it is used for food and/or medicinal purposes due to its analgesic, anti-inflammatory, anti-rheumatic and anti-arthritic properties; moreover, it is suitable to treat different skin diseases. However, its seeds present integumentary dormancy caused by uneven germination. The aim of the present study is to assess the effect of chemical scarification in sulfuric acid on dormancy overcome in Phytolacca Americana (Phytolaccaceae) seeds. Seeds were immersed in 4 ml sulfuric acid, which was concentrated through different immersion times (2, 5, 8, 11 and 15 min), and compared to the control treatment (without sulfuric acid). The study followed a completely randomized design with six treatments and four repetitions, with 50 seeds per treatment. The following parameters were assessed: days to start germination (DSG), germination (G), germination speed index (GSI), abnormal plantlets (AP), dead seeds (DS), hard seeds (HS) and total dry mass (TDM). Data were subjected to Shapiro-Wilk test for variance normality. The analysis of variance and regression were carried out in RStudio software, version 4.1.0. Means recorded for treatments were compared through Tukey test (p ≤ 0.05). The treatment based on immersion in sulfuric acid for 2 min was the most efficient in overcoming dormancy in this species, since it led to the best results for germination variables and total biomass accumulation in plantlets.


A Phytolacca americana (Phytolaccaceae) é uma espécie que pode ser classificada como PANC (Planta Alimentícia Não Convencional), utilizada para fins alimentícios e/ou medicinais com propriedades analgésicas, antiinflamatórias, antirreumáticas, antiartríticas e, também é adequada para o tratamento de várias doenças de pele. Contudo, suas sementes apresentam dormência tegumentar causando desuniformidade na germinação. Objetivou-se com o presente trabalho avaliar o efeito da escarificação química com ácido sulfúrico na superação da dormência em sementes de Phytolacca americana (Phytolaccaceae). As sementes foram imersas em 4 ml de ácido sulfúrico concentrado por diferentes períodos de imersão (2, 5, 8, 11 e 15 min) comparadas ao tratamento controle (sem ácido sulfúrico). O delineamento utilizado foi o inteiramente casualizado com seis tratamentos e quatro repetições de 50 sementes por tratamento. Foram avaliados os seguintes parâmetros: dias para iniciar a germinação (DIG), germinação (G), índice de velocidade de germinação (IVG), plântulas anormais (PA), sementes mortas (SM), sementes duras (SD), e massa seca total (MST). Os dados foram submetidos ao teste de Shapiro-Wilk para normalidade das variâncias. E a análise de variância e de regressão por meio do software RStudio versão 4.1.0. Sendo as médias dos tratamentos comparadas pelo teste Tukey (p ≤ 0,05). O tratamento de imersão em ácido sulfúrico por 2 min mostrou-se o mais eficiente na superação da dormência dessa espécie, proporcionando melhores resultados nas variáveis germinativas e acúmulo de biomassa total das plântulas.


Assuntos
Ácidos Sulfúricos/administração & dosagem , Germinação , Phytolacca/embriologia , Dormência de Plantas , Plantas Comestíveis , Brotos de Planta
19.
Rev. bras. ciênc. avic ; 23(1)fev. 2021.
Artigo em Inglês | LILACS-Express | VETINDEX | ID: biblio-1490845

Resumo

ABSTRACT The hypothalamic-pituitary-gonadal (HPG) axis is a key neuroen-docrine regulation system involved in the growth and reproduction of poultry. High-temperature conditions lead to the physiological dysfunction of target organs of the HPG axis of poultry, ultimately affecting the animals growth and development. In this study, we evaluated the effect of heat stress (HS) on the development of cells secreting major reproductive hormones of the HPG axis (i.e., hypothalamus, pituitary gland, ovary, and testis) of Wenchang chicks. Seventy-two one-day-old healthy Wenchang chicks were randomly divided into control (CK) and HS groups. The HS group was placed in a 40 ± 0.5°C artificial climate chamber for heat-stress treatment from 13:00 to 15:00 daily for six consecutive weeks. As development progressed, compared with the CK group, the gonadotropin-releasing hormone (GnRH), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) levels in the hypothalamus and testosterone (T) levers in the testes of male chicks in the HS group were significantly decreased at three weeks of age (p 0.05). However, GnRH levels in the hypothalamus and estradiol (E2) levels in the ovaries of female chicks in the HS group were variable and significantly lower than those of the CK group at four and five weeks of age (p 0.05). In addition, the LH and FSH levels in the pituitary gland were significantly lower than those in the CK group at two and four weeks of age and at four and six weeks of age, respectively (p 0.05). In brief, HS caused dysfunction of the corresponding target organs of the HPG axis in Wenchang chicks, and this affected the normal growth and development of the cells HPG axis.

20.
Anim. Reprod. (Online) ; 18(4): e20200255, 2021. graf
Artigo em Inglês | LILACS-Express | VETINDEX | ID: biblio-1349958

Resumo

Abstract Here, we aimed to discriminate between the spectral profiles of spent culture media after oocyte in vitro maturation (IVM) and culture (IVC) from goats of different ages subjected to repeated hormonal treatments. The profiles were discriminated using near infrared (NIR) spectroscopy combined with multivariate methods. A total of 19 goats (young = 10; old = 9) were subjected to serial hormonal stimulation (HS) with gonadotropins. Cumulus oophorus complexes (COCs) were collected using laparoscopic ovum pick-up (LOPU) and subjected to IVM and parthenogenetic activation. The initial embryos were subjected to IVC. Spent culture media were collected after oocyte IVM and on day 2 of IVC and analyzed using NIR spectroscopy. NIR spectral data were interpreted through chemometric methods, such as principle component analysis (PCA) and partial least square discriminant analysis (PLS-DA). The results of PCA analysis clearly showed a separation in the spectral profiles between the experimental groups (HS sessions; young and old animals) both after IVM and IVC. Overall, the main absorption bands were attributed to the C-H group second overtone, first overtone of O-H and N-H, and C-H combinations and may serve as molecular markers. On the other hand, the spectral data obtained using PLS-DA models provided a better classification of the groups. The results showed the possibility of discriminating young and old groups as well as the three HS sessions with high specificity, sensitivity, and accuracy using NIR spectra. Thus, the culture medium analysis using NIR spectroscopy combined with multivariate methods indicated the dissimilarities between the groups and provided an insight into the in vitro development of goat oocytes. This technique serves as an efficient, objective, rapid, and non-invasive method to discriminate spectral profiles.

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