O1-conotoxin Tx6. 7 cloned from the genomic DNA of Conus textile that inhibits calcium currents

Background: Conotoxins exhibit great potential as neuropharmacology tools and therapeutic candidates due to their high affinity and specificity for ion channels, neurotransmitter receptors or transporters. The traditional methods to discover new conotoxins are peptide purification from the crude venom or gene amplification from the venom duct. Methods: In this study, a novel O1 superfamily conotoxin Tx6.7 was directly cloned from the genomic DNA of Conus textile using primers corresponding to the conserved intronic sequence and 3' UTR elements. The mature peptide of Tx6.7 (DCHERWDW CPASLLGVIYCCEGLICFIAFCI) was synthesized by solid-phase chemical synthesis and confirmed by mass spectrometry. Results: Patch clamp experiments on rat DRG neurons showed that Tx6.7 inhibited peak calcium currents by 59.29 ± 2.34% and peak potassium currents by 22.33 ± 7.81%. In addition, patch clamp on the ion channel subtypes showed that 10 µM Tx6.7 inhibited 56.61 ± 3.20% of the hCaV1.2 currents, 24.67 ± 0.91% of the hCaV2.2 currents and 7.30 ± 3.38% of the hNaV1.8 currents. Tx6.7 had no significant toxicity to ND7/23 cells and increased the pain threshold from 0.5 to 4 hours in the mouse hot plate assay. Conclusion: Our results suggested that direct cloning of conotoxin sequences from the genomic DNA of cone snails would be an alternative approach to obtaining novel conotoxins. Tx6.7 could be used as a probe tool for ion channel research or a therapeutic candidate for novel drug development.(AU)

Morphometric and molecular differentiation of Pimelodus grosskopfii and Pimelodus yuma (Siluriformes: Pimelodidae)

Pimelodus grosskopfii and Pimelodus yuma, two species endemic to the Magdalena-Cauca basin in Colombia, overlap in the ranges of some of their diagnostic characters, which hampers their correct morphological identification. Aiming to help discriminate these species, this study conducted an integrative analysis using traditional and geometric morphometrics, phylogenetic analysis based on partial sequences of the mitochondrial cytochrome c oxidase subunit 1 gene (COI, cox1) and the identification of diagnostic Single Nucleotide Polymorphism markers (SNP). The species differ significantly in body geometry, allowing 100% discrimination, which was reinforced by a phylogenetic analysis that recovered well-supported monophyly of each species (posterior probability > 0.95). Additionally, the traditional morphometric results corroborated some previously reported diagnostic traits for both species and let us describe one non-overlapping ratio related to the adipose fin length. Three of five SNP markers had reciprocally exclusive alleles suitable for identifying each species. The morphometric and molecular methods conducted in this study constitute alternative tools for the correct discrimination of P. grosskopfii and P. yuma in the wild and in captive populations used for aquaculture.(AU)

Pimelodus grosskopfii y Pimelodus yuma, dos especies endémicas de la Cuenca Magdalena-Cauca en Colombia, se superponen en los rangos de variación de algunos de sus caracteres diagnósticos, lo que dificulta su correcta diferenciación morfológica. Con el objetivo de contribuir a la discriminación de estas especies, se realizó un análisis integrativo utilizando la morfometría geométrica y tradicional, análisis filogenético basado en secuencias parciales del gen mitocondrial citocromo c oxidasa subunidad I (COI, cox1) y la identificación de marcadores diagnósticos de polimorfismo de nucleótido único (SNP). Las especies difieren significativamente en la geometría del cuerpo, permitiendo una discriminación del 100%, lo que fue reforzado por un análisis filogenético que recuperó una monofilia bien soportada para cada especie (probabilidad posterior > 0,95). Además, los resultados de la morfometría tradicional corroboraron algunos rasgos diagnósticos previamente reportados para ambas especies y nos permitieron describir una proporción que no se sobrepone, relacionada con la longitud de la aleta adiposa. Tres de los cinco marcadores SNP poseían alelos recíprocamente exclusivos, adecuados para identificar cada especie. Los métodos morfométricos y moleculares implementados en este estudio constituyen herramientas alternativas para la correcta discriminación de P. grosskopfii y P. yuma tanto en la naturaleza como en poblaciones cautivas utilizadas para la acuicultura.(AU)

Dejeto líquido de suínos: efeito no solo e nas perdas de K, Ca e Mg por erosão hídrica / Pig slurry: effect on soil and K, Ca and Mg losses by water erosion

O dejeto de suíno (DLS) é usado como fertilizante cujos nutrientes são perdidos por erosão e contaminam o ambiente se o dejeto é usado inadequadamente. Objetivou-se avaliar o efeito de DLS nosolo e na erosão, num Nitossolo Bruno. Os tratamentos, 0; 50; 100; e 200 m³ ha-1de DLS foram aplicados superficialmente após a semeadura uma vez em cada um dos cinco cultivos, e na palhada do último cultivo, totalizando 0, 250, 500 e 1000 m³ ha-1, na aveia (Avena strigosa), milho (Zea mays), nabo (Raphanus sativus L.) e soja (Glycine max), e nos resíduos de aveia. A chuva simulada (65 mm h-1e 75 minutos) foi aplicada três vezes no milho e quatro vezes no nabo, na soja, e nos resíduos, com um simulador debraços rotativos. Antes e depois da pesquisa, determinou-se o teor de K, Ca e Mg no solo. As chuvas aplicadas em cada momento compuseram um teste (T). O T1 ocorreu após a aplicação do DLS e os demais testes em intervalos que variaram entre 14 e 70 dias, em função do clima. Durante o escoamento, em intervalos de cincominutos coletaram-se amostras de enxurrada para determinar as perdas de água e o teor de K, Ca e Mg na água. O DLS não influenciou o teor dos nutrientes no solo. O teore as perdas totais dos nutrientes foram maiores com 1000 m3ha-1de DLS do que na ausência do dejeto. Com o aumento de escoamento, diminuiu o teor na enxurrada e aumentou a perda total, a qual aumentou também com o aumento de teor dos nutrientes na enxurrada. Recomenda-se aplicar menos do que 500 m3ha-1de DLS total no solo, mesmo em aplicações intervalares, devido à elevada perda de K, Ca e Mg pela erosão hídrica.(AU)

Pigg slurry (PS) is used as a fertilizer whose nutrients are lost through erosion and contaminate the environment if the manure is used inappropriately. The objective was to evaluate the effect of PS on soil and erosion, in a Alfisol soil. The treatments, 0; 50; 100; and 200 m³ ha-1of PS were applied superficially after sowing once in each of the five crops, and in the straw of the last crop, totaling 0, 250, 500 and 1000 m³ ha-1, in oat (Avena strigosa), corn (Zea mays), turnip (Raphanus sativusL.) and soybean (Glycine max), and in oat residues. The simulated rain (65 mm h-1and 75 minutes) was applied three times to corn and four times to turnip, soybeans, and residues, with a rotating arms simulator. Before and after the research, the content of K, Ca and Mg in thesoil was determined. The rainfall applied at each moment made up a test (T). T1 occurred after the application of the PS and the other tests at intervals that varied between 14 and 70 days, depending on the weather. During the runoff, at five-minute intervals, runoff samples were collected to determine water losses and the content of K, Ca and Mg in the water. The PS did not influence the nutrient content in the soil. The content and total losses of nutrients were higher with 1000 m3ha-1of PS than in theabsence of manure. With the increase in runoff, the content inthe runoff decreased and the total loss increased, which also increased with the increase in the nutrient content in the runoff. It is recommended to apply less than 500 m3ha-1of total PS inthe soil, even in interval applications, due to the high loss of K, Ca and Mg by water erosion.(AU)

Glyceryl guaiacol ether for sedation of horses undergoing orchiectomy when standing / Éter gliceril-guaiacol para sedação de equinos submetidos à orquiectomia em pé

A study was designed to compare two sedation protocols to be used in horses undergoing orchiectomy when standing. In both protocols, the induction to the sedative state was performed with bolus detomidine at a dose of 10 µg/kg, intravenously (IV). In the first protocol (eight horses), the sedative state was maintained with 1% glyceryl guaiacol ether (GGE) in continuous infusion, at a dose of 1ml/kg/hour IV. In the second protocol (eight horses) this sedative effect was maintained with detomidine in continuous infusion at the same dose and induction route. Orchiectomy was performed on all animals. The two protocols allowed the surgeries to be performed when standing. However, horses kept under sedation by the GGE showed greater relaxation and a more intense degree of sedation. It should be noted that the use of GGE to maintain the sedative state in horses is unprecedented in the literature. The drug is used in anesthetic protocols in the species, but only in pre-anesthetic medication in general anesthesia. It was concluded that the two sedation protocols allowed the performance of orchiectomy with the horses when standing. However, the protocol in which 1% GGE was used showed more profound sedation, without adverse effects.

Foi realizado um estudo para comparar dois protocolos de sedação a serem utilizados em equinos submetidos à orquiectomia em estação. Em ambos os protocolos, a indução ao estado sedativo foi realizada com bolus de detomidina, na dose de 10µg/kg, por via intravenosa (IV). No primeiro protocolo (oito cavalos), o estado sedativo foi mantido com 1% de éter gliceril guaiacol (GGE) em infusão contínua, na dose de 1mL/kg/hora IV. No segundo protocolo (oito cavalos), esse efeito sedativo foi mantido com detomidina, em infusão contínua, na mesma dose e via de indução. Foi realizada orquiectomia em todos os animais. Os dois protocolos permitiram que as cirurgias fossem realizadas em estação No entanto, os cavalos mantidos sob sedação pelo GGE apresentaram maior relaxamento e grau de sedação mais intenso. Ressalta-se que o uso de GGE para manutenção do estado sedativo em equinos é inédito na literatura. O fármaco é utilizado em protocolos anestésicos na espécie, mas apenas na medicação pré-anestésica em anestesia geral. Concluiu-se que os dois protocolos de sedação permitiram a realização da orquiectomia com os cavalos em estação. No entanto, o protocolo em que foi utilizado GGE 1% apresentou sedação mais profunda, sem efeitos adversos.

Environmental characteristics, nutritional and executive functions in children of 6 to 7 years

Abstract This study aimed to analyze the socio-environmental characteristics, executive and nutritional functions in children aged 6 to 7 years, from public schools in Alagoas, Brazil. A quantitative, cross-sectional and descriptive study was performed involving 64 children of the mentioned age group, from public schools located in Alagoas: Maceió, on the coast; Major Isidoro, in the hinterland and Palmeira dos Índios, in the country. Such analyzes were made through the application of neuropsychological tests and anthropometric assessment with children and food and socio-environmental surveys with their parents. As for the type of housing, 100% were made of masonry, with a bathroom present in 98.4%. All children reported with the habit of bathing in the river / lagoon, presented some pathology. There was no significant difference between cities in terms of the sociodemographic characteristics evaluated, with the same result occurring with the factors associated with the occurrence of diseases in children. The subtests of WISC-IV, were below the average in all municipalities, and the TAC and SCC were classified within the average. However, even though the ranking were divided between below average and average, it is possible to identify from the subtests of WISC-IV, that the general IQ showed a cognitive level below the average. There was also no significant difference in the anthropometric assessment (weight, height, BMI and IMCI) between the evaluated students. The average weight was 23.3 kg to 25 kg, the height between 1.23 m to 1.24 m, the BMI between 16.4 to 17; the IMCI from 2.8 to 3.0. Children were classified within the average. Regarding micronutrients (Ca, Fe, K, Mg and Na, and vitamins A, C, D, B1, B9 and B12) and calories, there was also no significant difference between the cities evaluated. The same occurred with macronutrients (proteins, carbohydrates and lipids). This study showed that in general there was no difference between the students of the three municipalities. Probably, even though they are all public schools and from different cities, children have similar social conditions.

Resumo Este estudo teve como objetivo analisar as características socioambientais, funções executivas e nutricionais de crianças de 6 a 7 anos, de escolas públicas de Alagoas, Brasil. Foi realizado um estudo quantitativo, transversal e descritivo envolvendo 64 crianças, na referida faixa etária, de escolas públicas localizadas em Alagoas: Maceió, no litoral; Major Isidoro, no sertão e Palmeira dos Índios, no país. Tais análises foram feitas por meio da aplicação de testes neuropsicológicos e avaliação antropométrica com crianças e inquéritos alimentares e socioambientais com seus pais. Quanto ao tipo de habitação, 100% eram de alvenaria, com banheiro presente em 98,4%. Todas as crianças relataram o hábito de tomar banho no rio / lagoa, apresentavam alguma patologia. Não houve diferença significativa entre os municípios quanto às características sociodemográficas avaliadas, ocorrendo o mesmo resultado com os fatores associados à ocorrência de doenças em crianças. Os subtestes do WISC-IV, ficaram abaixo da média em todos os municípios, e o TAC e SCC foram classificados dentro da média. Porém, mesmo que a classificação tenha sido dividida entre abaixo da média e média, é possível identificar a partir dos subtestes do WISC-IV, que o QI geral apresentou um nível cognitivo abaixo da média. Também não houve diferença significativa na avaliação antropométrica (peso, altura, IMC e AIDPI) entre os alunos avaliados. O peso médio foi de 23,3 kg a 25 kg, a altura entre 1,23 ma 1,24 m, o IMC entre 16,4 a 17; A AIDPI de 2.8 a 3.0. As crianças foram classificadas dentro da média. Em relação aos micronutrientes (Ca, Fe, K, Mg e Na e vitaminas A, C, D, B1, B9 e B12) e calorias, também não houve diferença significativa entre os municípios avaliados. O mesmo ocorreu com os macronutrientes (proteínas, carboidratos e lipídios). Este estudo mostrou que de maneira geral não houve diferença entre os alunos dos três municípios. Provavelmente, mesmo sendo todas escolas públicas e de diferentes cidades, as crianças têm condições sociais semelhantes.

Amelioration of exosome and mesenchymal stem cells in rats infected with diabetic nephropathy by attenuating early markers and aquaporin-1 expression / Melhoria das células-tronco exossomais e mesenquimais em ratos infectados com nefropatia diabética atenuando marcadores precoces e expressão de aquaporina-1

Diabetic nephropathy (DN) is a prevalent diabetic microvascular condition. It is the leading cause of kidney disease in the advanced stages. There is no currently effective treatment available. This research aimed to investigate the curative potentials of exosomes isolated from mesenchymal stem cells affecting DN. This study was performed on 70 male adult albino rats. Adult rats were randomized into seven groups: Group I: Negative control group, Group II: DN group, Group III: Balanites treated group, Group IV: MSCs treated group, Group V: Exosome treated group, Group VI: Balanites + MSCs treated group and Group VII: Balanites + exosome treated group. Following the trial period, blood and renal tissues were subjected to biochemical, gene expression analyses, and histopathological examinations. Results showed that MDA was substantially increased, whereas TAC was significantly decreased in the kidney in the DN group compared to normal health rats. Undesired elevated values of MDA levels and a decrease in TAC were substantially ameliorated in groups co-administered Balanites aegyptiacae with MSCs or exosomes compared to the DN group. A substantial elevation in TNF-α and substantially diminished concentration of IGF-1 were noticed in DN rats compared to normal health rats. Compared to the DN group, the co-administration of Balanites aegyptiacae with MSCs or exosomes substantially improved the undesirable elevated values of TNF-α and IGF-1. Furthermore, in the DN group, the mRNA expression of Vanin-1, Nephrin, and collagen IV was significantly higher than in normal healthy rats. Compared with DN rats, Vanin-1, Nephrin, and collagen IV Upregulation were substantially reduced in groups co-administered Balanites aegyptiacae with MSCs or exosomes. In DN rats, AQP1 expression was significantly lower than in normal healthy rats. Furthermore, the groups co-administered Balanites aegyptiacae with MSCs or exosomes demonstrated a substantial increase in AQP1 mRNA expression compared to DN rats.

A nefropatia diabética (ND) é uma condição microvascular diabética prevalente. É a principal causa de doença renal em estágios avançados. Atualmente, não há tratamento eficaz disponível. Esta pesquisa teve como objetivo investigar os potenciais curativos de exossomos isolados de células-tronco mesenquimais que afetam a ND. Este estudo foi realizado em 70 ratos albinos adultos machos. Ratos adultos foram randomizados em sete grupos: Grupo I: Grupo de controle negativo, Grupo II: Grupo DN, Grupo III: Grupo tratado com Balanites, Grupo IV: Grupo tratado com MSCs, Grupo V: Grupo tratado com exossomos, Grupo VI: Grupo tratado com Balanites + MSCs e Grupo VII: Balanites + grupo tratado com exossomas. Após o período experimental, o sangue e os tecidos renais foram submetidos a análises bioquímicas, de expressão gênica e exames histopatológicos. Os resultados mostraram que o MDA aumentou substancialmente, enquanto o TAC diminuiu significativamente no rim no grupo DN em comparação com ratos saudáveis normais. Valores elevados indesejados de níveis de MDA e uma diminuição no TAC foram substancialmente melhorados em grupos coadministrados Balanites aegyptiacae com MSCs ou exossomas em comparação com o grupo DN. Uma elevação substancial em TNF-α e uma concentração substancialmente diminuída de IGF-1 foram observadas em ratos DN em comparação com ratos saudáveis normais. Em comparação com o grupo DN, a coadministração de Balanites aegyptiacae com MSCs ou exossomas melhorou substancialmente os valores elevados indesejáveis de TNF-α e IGF-1. Além disso, no grupo DN a expressão de mRNA de vanina-1, nefrina e colágeno IV foi significativamente maior do que em ratos saudáveis normais. Comparado com ratos DN, Vanin-1, Nephrin e colágeno IV Upregulation foram substancialmente reduzidos em grupos co-administrados Balanites aegyptiacae com MSCs ou exossomos. Em ratos DN, a expressão de AQP1 foi significativamente menor do que em ratos saudáveis normais. Além disso, os grupos que coadministraram Balanites aegyptiacae com MSCs ou exossomos demonstraram um aumento substancial na expressão de mRNA de AQP1 em comparação com ratos DN.

Environmental characteristics, nutritional and executive functions in children of 6 to 7 years / Características ambientais, funções nutricionais e executivas em crianças de 6 a 7 anos

This study aimed to analyze the socio-environmental characteristics, executive and nutritional functions in children aged 6 to 7 years, from public schools in Alagoas, Brazil. A quantitative, cross-sectional and descriptive study was performed involving 64 children of the mentioned age group, from public schools located in Alagoas: Maceió, on the coast; Major Isidoro, in the hinterland and Palmeira dos Índios, in the country. Such analyzes were made through the application of neuropsychological tests and anthropometric assessment with children and food and socio-environmental surveys with their parents. As for the type of housing, 100% were made of masonry, with a bathroom present in 98.4%. All children reported with the habit of bathing in the river / lagoon, presented some pathology. There was no significant difference between cities in terms of the sociodemographic characteristics evaluated, with the same result occurring with the factors associated with the occurrence of diseases in children. The subtests of WISC-IV, were below the average in all municipalities, and the TAC and SCC were classified within the average. However, even though the ranking were divided between below average and average, it is possible to identify from the subtests of WISC-IV, that the general IQ showed a cognitive level below the average. There was also no significant difference in the anthropometric assessment (weight, height, BMI and IMCI) between the evaluated students. The average weight was 23.3 kg to 25 kg, the height between 1.23 m to 1.24 m, the BMI between 16.4 to 17; the IMCI from 2.8 to 3.0. Children were classified within the average. Regarding micronutrients (Ca, Fe, K, Mg and Na, and vitamins A, C, D, B1, B9 and B12) and calories, there was also no significant difference between the cities evaluated. The same occurred with macronutrients (proteins, carbohydrates and lipids). This study showed [...].

Este estudo teve como objetivo analisar as características socioambientais, funções executivas e nutricionais de crianças de 6 a 7 anos, de escolas públicas de Alagoas, Brasil. Foi realizado um estudo quantitativo, transversal e descritivo envolvendo 64 crianças, na referida faixa etária, de escolas públicas localizadas em Alagoas: Maceió, no litoral; Major Isidoro, no sertão e Palmeira dos Índios, no país. Tais análises foram feitas por meio da aplicação de testes neuropsicológicos e avaliação antropométrica com crianças e inquéritos alimentares e socioambientais com seus pais. Quanto ao tipo de habitação, 100% eram de alvenaria, com banheiro presente em 98,4%. Todas as crianças relataram o hábito de tomar banho no rio / lagoa, apresentavam alguma patologia. Não houve diferença significativa entre os municípios quanto às características sociodemográficas avaliadas, ocorrendo o mesmo resultado com os fatores associados à ocorrência de doenças em crianças. Os subtestes do WISC-IV, ficaram abaixo da média em todos os municípios, e o TAC e SCC foram classificados dentro da média. Porém, mesmo que a classificação tenha sido dividida entre abaixo da média e média, é possível identificar a partir dos subtestes do WISC-IV, que o QI geral apresentou um nível cognitivo abaixo da média. Também não houve diferença significativa na avaliação antropométrica (peso, altura, IMC e AIDPI) entre os alunos avaliados. O peso médio foi de 23,3 kg a 25 kg, a altura entre 1,23 ma 1,24 m, o IMC entre 16,4 a 17; A AIDPI de 2.8 a 3.0. As crianças foram classificadas dentro da média. Em relação aos micronutrientes (Ca, Fe, K, Mg e Na e vitaminas A, C, D, B1, B9 e B12) e calorias, também não houve diferença significativa entre os municípios avaliados. O mesmo ocorreu com os macronutrientes (proteínas, carboidratos e lipídios). Este estudo mostrou que de maneira geral não houve diferença entre os alunos [...].

Environmental characteristics, nutritional and executive functions in children of 6 to 7 years / Características ambientais, funções nutricionais e executivas em crianças de 6 a 7 anos

Abstract This study aimed to analyze the socio-environmental characteristics, executive and nutritional functions in children aged 6 to 7 years, from public schools in Alagoas, Brazil. A quantitative, cross-sectional and descriptive study was performed involving 64 children of the mentioned age group, from public schools located in Alagoas: Maceió, on the coast; Major Isidoro, in the hinterland and Palmeira dos Índios, in the country. Such analyzes were made through the application of neuropsychological tests and anthropometric assessment with children and food and socio-environmental surveys with their parents. As for the type of housing, 100% were made of masonry, with a bathroom present in 98.4%. All children reported with the habit of bathing in the river / lagoon, presented some pathology. There was no significant difference between cities in terms of the sociodemographic characteristics evaluated, with the same result occurring with the factors associated with the occurrence of diseases in children. The subtests of WISC-IV, were below the average in all municipalities, and the TAC and SCC were classified within the average. However, even though the ranking were divided between below average and average, it is possible to identify from the subtests of WISC-IV, that the general IQ showed a cognitive level below the average. There was also no significant difference in the anthropometric assessment (weight, height, BMI and IMCI) between the evaluated students. The average weight was 23.3 kg to 25 kg, the height between 1.23 m to 1.24 m, the BMI between 16.4 to 17; the IMCI from 2.8 to 3.0. Children were classified within the average. Regarding micronutrients (Ca, Fe, K, Mg and Na, and vitamins A, C, D, B1, B9 and B12) and calories, there was also no significant difference between the cities evaluated. The same occurred with macronutrients (proteins, carbohydrates and lipids). This study showed that in general there was no difference between the students of the three municipalities. Probably, even though they are all public schools and from different cities, children have similar social conditions.

Resumo Este estudo teve como objetivo analisar as características socioambientais, funções executivas e nutricionais de crianças de 6 a 7 anos, de escolas públicas de Alagoas, Brasil. Foi realizado um estudo quantitativo, transversal e descritivo envolvendo 64 crianças, na referida faixa etária, de escolas públicas localizadas em Alagoas: Maceió, no litoral; Major Isidoro, no sertão e Palmeira dos Índios, no país. Tais análises foram feitas por meio da aplicação de testes neuropsicológicos e avaliação antropométrica com crianças e inquéritos alimentares e socioambientais com seus pais. Quanto ao tipo de habitação, 100% eram de alvenaria, com banheiro presente em 98,4%. Todas as crianças relataram o hábito de tomar banho no rio / lagoa, apresentavam alguma patologia. Não houve diferença significativa entre os municípios quanto às características sociodemográficas avaliadas, ocorrendo o mesmo resultado com os fatores associados à ocorrência de doenças em crianças. Os subtestes do WISC-IV, ficaram abaixo da média em todos os municípios, e o TAC e SCC foram classificados dentro da média. Porém, mesmo que a classificação tenha sido dividida entre abaixo da média e média, é possível identificar a partir dos subtestes do WISC-IV, que o QI geral apresentou um nível cognitivo abaixo da média. Também não houve diferença significativa na avaliação antropométrica (peso, altura, IMC e AIDPI) entre os alunos avaliados. O peso médio foi de 23,3 kg a 25 kg, a altura entre 1,23 ma 1,24 m, o IMC entre 16,4 a 17; A AIDPI de 2.8 a 3.0. As crianças foram classificadas dentro da média. Em relação aos micronutrientes (Ca, Fe, K, Mg e Na e vitaminas A, C, D, B1, B9 e B12) e calorias, também não houve diferença significativa entre os municípios avaliados. O mesmo ocorreu com os macronutrientes (proteínas, carboidratos e lipídios). Este estudo mostrou que de maneira geral não houve diferença entre os alunos dos três municípios. Provavelmente, mesmo sendo todas escolas públicas e de diferentes cidades, as crianças têm condições sociais semelhantes.

Environmental characteristics, nutritional and executive functions in children of 6 to 7 years / Características ambientais, funções nutricionais e executivas em crianças de 6 a 7 anos

This study aimed to analyze the socio-environmental characteristics, executive and nutritional functions in children aged 6 to 7 years, from public schools in Alagoas, Brazil. A quantitative, cross-sectional and descriptive study was performed involving 64 children of the mentioned age group, from public schools located in Alagoas: Maceió, on the coast; Major Isidoro, in the hinterland and Palmeira dos Índios, in the country. Such analyzes were made through the application of neuropsychological tests and anthropometric assessment with children and food and socio-environmental surveys with their parents. As for the type of housing, 100% were made of masonry, with a bathroom present in 98.4%. All children reported with the habit of bathing in the river / lagoon, presented some pathology. There was no significant difference between cities in terms of the sociodemographic characteristics evaluated, with the same result occurring with the factors associated with the occurrence of diseases in children. The subtests of WISC-IV, were below the average in all municipalities, and the TAC and SCC were classified within the average. However, even though the ranking were divided between below average and average, it is possible to identify from the subtests of WISC-IV, that the general IQ showed a cognitive level below the average. There was also no significant difference in the anthropometric assessment (weight, height, BMI and IMCI) between the evaluated students. The average weight was 23.3 kg to 25 kg, the height between 1.23 m to 1.24 m, the BMI between 16.4 to 17; the IMCI from 2.8 to 3.0. Children were classified within the average. Regarding micronutrients (Ca, Fe, K, Mg and Na, and vitamins A, C, D, B1, B9 and B12) and calories, there was also no significant difference between the cities evaluated. The same occurred with macronutrients (proteins, carbohydrates and lipids). This study showed [...].(AU)

Este estudo teve como objetivo analisar as características socioambientais, funções executivas e nutricionais de crianças de 6 a 7 anos, de escolas públicas de Alagoas, Brasil. Foi realizado um estudo quantitativo, transversal e descritivo envolvendo 64 crianças, na referida faixa etária, de escolas públicas localizadas em Alagoas: Maceió, no litoral; Major Isidoro, no sertão e Palmeira dos Índios, no país. Tais análises foram feitas por meio da aplicação de testes neuropsicológicos e avaliação antropométrica com crianças e inquéritos alimentares e socioambientais com seus pais. Quanto ao tipo de habitação, 100% eram de alvenaria, com banheiro presente em 98,4%. Todas as crianças relataram o hábito de tomar banho no rio / lagoa, apresentavam alguma patologia. Não houve diferença significativa entre os municípios quanto às características sociodemográficas avaliadas, ocorrendo o mesmo resultado com os fatores associados à ocorrência de doenças em crianças. Os subtestes do WISC-IV, ficaram abaixo da média em todos os municípios, e o TAC e SCC foram classificados dentro da média. Porém, mesmo que a classificação tenha sido dividida entre abaixo da média e média, é possível identificar a partir dos subtestes do WISC-IV, que o QI geral apresentou um nível cognitivo abaixo da média. Também não houve diferença significativa na avaliação antropométrica (peso, altura, IMC e AIDPI) entre os alunos avaliados. O peso médio foi de 23,3 kg a 25 kg, a altura entre 1,23 ma 1,24 m, o IMC entre 16,4 a 17; A AIDPI de 2.8 a 3.0. As crianças foram classificadas dentro da média. Em relação aos micronutrientes (Ca, Fe, K, Mg e Na e vitaminas A, C, D, B1, B9 e B12) e calorias, também não houve diferença significativa entre os municípios avaliados. O mesmo ocorreu com os macronutrientes (proteínas, carboidratos e lipídios). Este estudo mostrou que de maneira geral não houve diferença entre os alunos [...].(AU)

Toxicological effects of thimerosal on rat kidney: a histological and biochemical study

Abstract Thimerosal is an organomercurial compound, which is used in the preparation of intramuscular immunoglobulin, antivenoms, tattoo inks, skin test antigens, nasal products, ophthalmic drops, and vaccines as a preservative. In most of animal species and humans, the kidney is one of the main sites for mercurial compounds deposition and target organs for toxicity. So, the current research was intended to assess the thimerosal induced nephrotoxicity in male rats. Twenty-four adult male albino rats were categorized into four groups. The first group was a control group. Rats of Group-II, Group-III, and Group-IV were administered with 0.5µg/kg, 10µg/kg, and 50µg/kg of thimerosal once a day, respectively. Thimerosal administration significantly decreased the activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), glutathione reductase (GR), glutathione (GSH), and protein content while increased the thiobarbituric acid reactive substances (TBARS) and hydrogen peroxide (H2O2) levels dose-dependently. Blood urea nitrogen (BUN), creatinine, urobilinogen, urinary proteins, kidney injury molecule-1 (KIM-1), and neutrophil gelatinase-associated lipocalin (NGAL) levels were substantially increased. In contrast, urinary albumin and creatinine clearance was reduced dose-dependently in thimerosal treated groups. The results demonstrated that thimerosal significantly increased the inflammation indicators including nuclear factor kappaB (NF-B), tumor necrosis factor- (TNF-), Interleukin-1 (IL-1), Interleukin-6 (IL-6) levels and cyclooxygenase-2 (COX-2) activities, DNA and histopathological damages dose-dependently. So, the present findings ascertained that thimerosal exerted nephrotoxicity in male albino rats.

Resumo O timerosal é um composto organomercurial, utilizado na preparação de imunoglobulina intramuscular, antivenenos, tintas de tatuagem, antígenos de teste cutâneo, produtos nasais, gotas oftálmicas e vacinas como conservante. Na maioria das espécies animais e nos humanos, o rim é um dos principais locais de deposição de compostos de mercúrio e órgãos-alvo de toxicidade. Assim, a presente pesquisa teve como objetivo avaliar a nefrotoxicidade induzida pelo timerosal em ratos machos. Vinte e quatro ratos albinos machos adultos foram categorizados em quatro grupos. O primeiro grupo era um grupo de controle. Ratos do Grupo II, Grupo III e Grupo IV receberam 0,5µg / kg, 10µg / kg e 50µg / kg de timerosal uma vez ao dia, respectivamente. A administração de timerosal diminuiu significativamente as atividades de catalase (CAT), superóxido dismutase (SOD), peroxidase (POD), glutationa redutase (GR), glutationa (GSH) e conteúdo de proteína, enquanto aumentou as substâncias reativas ao ácido tiobarbitúrico (TBARS) e peróxido de hidrogênio (H2O2) níveis dependentes da dose. Os níveis de nitrogênio ureico no sangue (BUN), creatinina, urobilinogênio, proteínas urinárias, molécula de lesão renal-1 (KIM-1) e lipocalina associada à gelatinase de neutrófilos (NGAL) aumentaram substancialmente. Em contraste, a albumina urinária e a depuração da creatinina foram reduzidas de forma dependente da dose nos grupos tratados com timerosal. Os resultados demonstraram que o timerosal aumentou significativamente os indicadores de inflamação, incluindo fator nuclear kappaB (NF-B), fator de necrose tumoral- (TNF-), interleucina-1 (IL-1), níveis de interleucina-6 (IL-6) e atividades da ciclooxigenase-2 (COX-2), DNA e danos histopatológicos dependentes da dose. Portanto, os presentes achados verificaram que o timerosal exerceu nefrotoxicidade em ratos albinos machos.

Caracterização, aptidão agrícola e capacidade de uso de um Argissolo Vermelho-Amarelo Eutrófico abrúptico: Estudo de caso em Itapetinga-BA / Characterization, agricultural aptitude and use capacity of 'Argissolo Vermelho-Amarelo Eutrófico abrúptico: a case study in Itapetinga -BA

No município de Itapetinga,90% das terras são ocupadas com pecuária e os Argissolos são os solos mais comuns. A pequena capacidade protetora das pastagens e também o adensamento dos rebanhos, tem causado severa compactação no solo, que se torna preocupante.Diante disso, os objetivos da pesquisa foram: i) caracterizar morfológica, física e quimicamente um Argissolo Vermelho-Amarelo; ii) indicar a aptidão agrícola e capacidade de uso. Realizou-se a caracterização morfológica do solo e coletaram-se amostras dos horizontes para caracterização física equímica. Fisicamente determinou-se a granulometria e argila dispersa em água (ADA). Quimicamente foram analisados os cátions trocáveis do complexo sortivo (Ca2+, Mg2+Na+, K+, Al3+e H+), pH, carbono, nitrogênio total e fósforo disponível,bem como, óxidos Al2O3e Fe2O3 por ataque sulfúrico. Os sistemas de Avaliação de Aptidão Agrícola das Terras e de Capacidade de Uso foram as metodologias utilizadas para essas classificações. A descrição morfológica e dados analíticos permitiram classificar o solo como Argissolo Vermelho-Amarelo Eutrófico abrúptico. Quanto à aptidão agrícola e capacidade de uso, o solo foi classificado como 1abC e IV E-3, respectivamente. Conclui-se que o uso atual está inadequado, recomendando-sepastagens perenes associadas a práticas conservacionistas que evitem a erosão do solo. O caráter abrúptico, aliado à compactação provocada pela pecuária extensiva, agrava ainda mais a perda da capacidade produtiva desse solo, condição representativa para a microrregião de Itapetinga, onde a maiorparte do seu território é coberto por Argissolos Vermelho-Amarelos Eutróficos, sendo cerca de 41%.(AU)

The livestock agriculture fromItapetinga are in area ofArgissolos. Themanagement of livestock has caused compaction. Thus, the purpose of the study was: i) describe the morphological, Chemical, and physical of the Argissolo Vermelho-Amarelo; ii) classify your agricultural aptitude and use capacity. The morphological characterization was described: depth, color, structure, texture, consistency, and transition. The physical analysis was: texture and clay dispersed in water. The Chemical analysis determined the cations (Ca2+, Mg2+ Na+, K+, Al3+ e H+), pH, carbon, nitrogen and phosphorus. The oxides Al2O3and Fe2O3were determined. The "Sistema de Avaliação de Aptidão Agrícola das Terras" and "Sistema de Capacidade de Uso" were used for classification. The soil was classified with Argissolo Vermelho-Amarelo Eutrófico abrúptico. Your aptitude has been graded as 1abC and IV E-3 to use capacity. Therefore, the use of soil is incorrect, because the perennial pasture with practices against erosion is the correct form of use. The abrupt characterwith compaction by the animals result in losses of the productive capacity of the soil.This is reality of the microregion of Itapetingabecausemost ofthe territory is in area of Argissolos Vermelho-Amarelos Eutróficos, totaling 41% of the total area.(AU)

Astragaloside IV alleviates stroke-triggered early brain injury by modulating neuroinflammation and ferroptosis via the Nrf2/HO-1 signaling pathway

Purpose: Stroke is an acute cerebrovascular disease. Astragaloside IV (AS-IV) is an active ingredient extracted from Astragalus membranaceus with an established therapeutic effect on central nervous system diseases. This study examined the neuroprotective properties and possible mechanisms of AS-IV in stroke-triggered early brain injury (EBI) in a rat transient middle cerebral artery occlusion (MCAO) model. Methods: The neurological scores and brain water content were analyzed. 2,3,5-triphenyl tetrazolium chloride (TTC) staining was utilized to determine the infarct volume, neuroinflammatory cytokine levels, and ferroptosis-related genes and proteins, and neuronal damage and molecular mechanisms were evaluated by terminal deoxynucleotidyl transferase dutp nickend labeling (TUNEL) staining, western blotting, and real-time polymerase chain reaction. Results: AS-IV administration decreased the infarct volume, brain edema, neurological deficits, and inflammatory cytokines TNF-α, interleukin-1ß (IL-1ß), IL-6, and NF-κB, increased the levels of SLC7A11 and glutathione peroxidase 4 (GPX4), decreased lipid reactive oxygen species (ROS) levels, and prevented neuronal ferroptosis. Meanwhile, AS-IV triggered the Nrf2/HO-1 signaling pathway and alleviated ferroptosis due to the induction of stroke. Conclusion: Hence, the findings of this research illustrate that AS-IV administration can improve delayed ischemic neurological deficits and decrease neuronal death by modulating nuroinflammation and ferroptosis via the Nrf2/HO-1 signaling pathway.

Resistance of gastrointestinal nematoids of goats and sheep to the antihelmintics levamisol, ivermectin and albendazole / Resistência de nematoides gastrintestinais de caprinos e ovinos aos anti-helmínticos levamisol, ivermectina e albendazol

The aim of this study was to determine the resistance of gastrointestinal nematodes in goats and sheep to the anthelmintic drugs levamisole, ivermectin, and albendazole in the metropolitan region of São Luís Island, Maranhão, Brazil. Fecal samples were collected from 150 animals across four different farms; two farms had goats, and the other two had sheep. The samples were then randomly divided into three to four groups of 10 animals: Group I: control, without treatment; Group II: ivermectin treatment; Group III: levamisole treatment; and Group IV: albendazole treatment. Stool samples were collected from the rectal ampulla one day before treatment and 10 days after anthelmintic treatment. Individual coproparasitological examinations were performed using the modified McMaster technique at the Animal Health Laboratory of the Federal Institute of Maranhão, Campus São Luís-Maracanã. The efficacies of the anthelmintic drugs against gastrointestinal nematodes in goats and sheep were: 14.28%, and 13.6% for ivermectin; 0% and 79.4% for levamisole; and 59.8% and 3.43% for albendazole, respectively Gastrointestinal nematodes demonstrated multiple anthelmintic resistance, as the percentage reduction in egg count was less than 95% and the lower limit of the confidence interval was less than 90%.

Objetivou-se determinar a resistência de nematoides gastrintestinais aos anti-helmínticos levamisol, ivermectina e albendazol em caprinos e ovinos da região metropolitana da Ilha de São Luís, Maranhão, Brasil. Foram coletadas amostras de fezes de 150 animais de quatro propriedades diferentes, sendo 2 com caprinos e 2 com ovinos, e aleatoriamente distribuídos de três a quatro grupos de 10 animais: Grupo I: grupo controle, sem tratamento. Grupo II: tratado com anti-helmíntico à base de ivermectina, administrado oralmente na dose de 200 mcg/kg; Grupo III: tratado com anti-helmíntico à base de levamisol, administrado oralmente na dose de 7,5mg/kg e Grupo IV: tratado com anti-helmíntico à base de albendazol administrado oralmente na dose de 3mg/kg. Amostras de fezes foram colhidas da ampola retal um dia antes do tratamento e 10 dias após o tratamento anti-helmíntico. Foram feitos exames coproparasitológicos individuais, pela técnica de McMaster modificada, no Laboratório de Sanidade Animal, do Instituto Federal do Maranhão (IFMA), Campus São Luís-Maracanã. A ivermectina, na espécie caprina, mostrou eficácia de 14,28%, enquanato para ovina, 13,6 e 52,2%. Considerando o levamisol na espécie caprina, não apresentou eficácia contra os nematoides gastrintestinais, enquanto para ovinos, apresentou eficácia de 79,4%. Já o albendazol, apresentou eficácia de 59,8% para caprinos, e 3,43% para ovinos. Os nematoides gastrintestinais demonstraram resistência múltipla (RAM), visto que a percentagem de redução da contagem de ovos foi inferior a 95% e o limite inferior do intervalo de confiança menor do que 90%.

The impact of agricultural comprehensive development on agro-ecological efficiency of China: evidence from perspective of scale and structure / O impacto do desenvolvimento agrícola na eficiência agro-ecológica da China: evidência sob a perspectiva de escala e estrutura

Promoting the agricultural sustainable development is one of the core objectives of agricultural comprehensive development (ACD), and the core of agricultural sustainable development lies in enhancing agro-ecological efficiency (AEE). Based on inter-provincial panel data from 2003-2017, the AEE was measured by a unexpected super-efficient SBM model. From the perspective of investment scale and investment structure, the impact of investment scale in ACD on AEE was examined using a panel econometric model, then characterizing the investment structure by the proportion of government funds in the investment of ACD, examining the non-linear relationship between investment structure of ACD and AEE seeking a reasonable proportion of government expenditure. Finally, a spatial econometric model was constructed to test the spatial spillover effect of the scale and structure of investment in ACD on AEE. Results showed that: (i) AEE of China is on the rise overall, but the efficiency level is still low and there is large scope for improvement. (ii) Due to the inefficient use of funds and the lag in the transformation of agricultural benefits, the scale of investment in ACD has a significant negative impact on AEE in the current period. (iii) The impact of investment structure of ACD on AEE presents a significant "inverted N" relationship, and the optimal proportion of government fund investment structure is 76.71%. The reasonable structure of investment in ACD at different food functional areas shows differences. (iv) After considering the spatial effect, the impact of ACD remains robust, but the spatial spillover effect prolongs the time lag of this impact of investment scale and starts to have a positive impact in the 2nd year after the investment. Guarantee the investment scale in ACD, reduce financial redundancy, establish a cooperation mechanism between ACD and agricultural ecological protection in neighboring areas, and increase the introduction of private capital can achieve sustainable development of agricultural economy.

Promover o desenvolvimento agrícola sustentável é um dos objetivos centrais do desenvolvimento agrícola abrangente (ACD), e o núcleo do desenvolvimento agrícola sustentável está no aumento da eficiência agro-ecológica (AEE). Com base nos dados do painel interprovincial de 2003-2017, o AEE foi medido por um modelo SBM super-eficiente inesperado. Da perspectiva da escala de investimento e da estrutura de investimento, o impacto da escala de investimento da ACD na AEE foi examinado utilizando um modelo econométrico de painel, então caracterizado a estrutura de investimento pela proporção de fundos governamentais no investimento da ACD, examina a relação não linear entre a estrutura de investimento da ACD e da AEE para buscar uma proporção razoável dos gastos governamentais. Finalmente, um modelo econométrico espacial foi construído para testar o efeito de spillover espacial da escala e da estrutura de investimento da TDAA na AEE. Os resultados mostram isso: (i) O AEE da China está em ascensão em geral, mas o nível de eficiência ainda é baixo e há uma grande margem para melhorias. (ii) Devido ao uso ineficiente dos fundos e ao atraso na transformação dos benefícios agrícolas, a escala de investimento na TCAA tem um impacto negativo significativo na TCAA no período atual. (iii) O impacto da estrutura de investimento da DCA na AEE apresenta uma relação "N invertida" significativa, e a proporção ótima da estrutura de investimento dos fundos governamentais é de 76,71%. A estrutura razoável de investimento na TCA em diferentes áreas funcionais de alimentos mostra diferenças. (iv) Depois de considerar o efeito espacial, o impacto da TCA permanece robusto, mas o efeito de spillover espacial prolonga o tempo de atraso deste impacto da escala de investimento e começa a ter um impacto positivo no 2º ano após o investimento. Garantir a escala de investimento na TCA, reduzir a redundância financeira, estabelecer um mecanismo de cooperação entre a TCA e a proteção ecológica agrícola em áreas vizinhas e aumentar a introdução de capital privado pode alcançar o desenvolvimento sustentável da economia agrícola.

Environmental variability in simultaneous selection of common bean for grain quality and mineral concentration traits

The selection of common bean (Phaseolus vulgaris L.) lines based on grain quality and mineral concentration traits will be more efficient if a minimum number of experiments is established. This study was carried out to determine whether grain quality and mineral concentration traits are significantly affected by the genotype × environment interaction; to estimate heritability and genetic gain in individual and combined experiments; and to select superior common bean lines considering a minimum number of experiments. A total of 17 common bean genotypes were evaluated in four experiments. Grain quality was determined through seven traits, and the concentration of six minerals was analyzed by acid digestion. Statistical analyses were completed using data obtained from both individual (I, II, III and IV) and combined (I and II; I, II and III; and I, II, III and IV) experiments. Except for the potassium concentration, all traits showed a significant genotype × environment interaction effect. Heritability and genetic gain estimates of grain quality and mineral concentration traits varied when the data were obtained from one or more experiments. Genetic gain may be inflated because of the data being based on one or two experiments. Four carioca bean (BRS MG Uai, LP 09-33, LEC 01-16 and Pérola) and four black bean (TB 02-19, CHP 04-239-52, TB 03-11 and IAC Netuno) genotypes were selected for their high grain quality and mineral concentration based on four experiments. Data from at least four experiments should be used to select common bean lines superior in grain quality and mineral concentration traits to increase the efficiency of simultaneous selection.

Crescimento, severidade de oídio e produtividade do feijão-vagem em resposta a diferentes fontes de potássio / Growth, powdery mildew severity and yield of green beans in response to potassium sources

O Brasil tem enfrentado sérios problemas com o abastecimento de fertilizantes potássicos nos últimos anos. Isso tem causado a elevação dos preços e até mesmo escassez do principal fertilizante potássico utilizado pelos produtores, o cloreto de potássio. Por isso, o objetivo do presente trabalho foi avaliar um silicato de potássio nacional (K6) como fonte alternativa ao uso do cloreto de potássio para a cultura do feijão-vagem. Para tanto, sementes de feijão-vagem cv. Macarrão Rasteiro foram semeadas a campo em espaçamento de 0,50 x 0,20 m. Empregou-se o delineamento em blocos ao acaso com quatro tratamentos e cinco repetições. Os tratamentos foram constituídos da seguinte maneira: (i) testemunha ­ sem adubação potássica; (ii) cloreto de potássio; (iii) silicato de potássio ­ K6; (iv) 50% cloreto de potássio + 50% K6. Os fertilizantes foram aplicados no momento da semeadura e as doses determinadas com base na análise de solo e nas recomendações para a cultura. Foi realizada avaliação de altura de plantas, massa fresca e seca da parte aérea, massa fresca e seca de vagens, estimativa de produtividade e ocorrência de oídio, através de notas de severidade. Verificou-se que não houve influência da adubação potássica sobre o crescimento das plantas. De maneira geral, a produtividade de vagens apresentou baixa influência da adubação. Entretanto, a produtividade total de vagens foi maior em plantas adubadas com K6 em comparação a plantas adubadas com cloreto de potássio. A aplicação de K6 também contribuiu para a redução da severidade de oídio. Dessa forma, nós concluímos que o K6 é uma fonte de potássio viável em substituição ao cloreto de potássio para o feijão-vagem. (AU)

Brazil has faced serious problems with the supply of potassium fertilizers in recent years. This has caused prices to rise and even shortages of the main potassium fertilizer used by producers, potassium chloride. Therefore, the objective of the present work was to evaluate a national potassium silicate (K6) as an alternative source to the use of potassium chloride for the green beans crop. For this purpose, green beans seeds cv. Macarrão Rasteiro were sown in the field at a spacing of 0.50 x 0.20 m. A randomized block design with four treatments and five replications was used. The treatments were constituted as follows: (i) control ­ without potassium fertilization; (ii) potassium chloride; (iii) potassium silicate - K6; (iv) 50% potassium chloride + 50% K6. Fertilizers were applied at the time of sowing and doses were determined based on soil analysis and recommendations for the crop. Plant height, shoot fresh and dry mass, pod fresh and dry mass, yield estimation and occurrence of powdery mildew were evaluated through severity scores. It was found that in terms of plant growth, there was no influence of potassium fertilization on plant growth. In addition, pod productivity showed a reduction throughout the evaluation period and little influence of fertilization. However, total pod productivity was higher in plants that received K6 compared to plants fertilized with potassium chloride. The application of K6 also contributed to the reduction of powdery mildew severity. Thus, we conclude that K6 is a viable source of potassium in substitution of potassium chloride for the green beans.(AU)

Milk allergy in jersey cows: first report in Brazil / Auto alergia ao leite em vaca jersey: primeiro relato no Brasil / Alergia a la leche de auto en vaca jersey: primer reporte en Brasil

Allergy to milk is the only auto-allergic disease in cattle. It is characterized by the retention and absorption of milk itself, making milk proteins, especially alpha casein, have access to the bloodstream, resulting in a type I hypersensitivity. The purpose of this report is to describe a case of auto-allergic disease in cattle. Here we report the case of a four-year-old Jersey female bovine, which was pregnant and weighing 400Kg. The animal was being treated at (hidden for revision)and had a history of an acute allergic reaction. Its owner reported sudden onset of skin lesions, salivation, and difficulty breathing. As the animal was participating in an agricultural exhibition, it was not being milked to generate mammary gland engorgement (milk retention). Inphysical examination findings wherein there is presence of multifocal urticarial papules and plaques, mainly on the sides and right and left flanks, besides the absence of pruritus, edema in mucocutaneous regions of the upper and lower eyelids of the right and left eyes, submandibular edema, excessive salivation, inspiratory dyspnea with half-open mouth breathing, respiratory distress, apathy, cough, tachycardia tachypnea, ruminal hypomotility and increased volume of the mammary gland, characterizing milk retention. Clinical suspicion was milk allergy with anaphylactic reaction (type I hypersensitivity). Treatment was based on promethazine administration (1mg/kg; IV) and immediate full milking of the mammary gland. After 12 h of treatment, clinical respiratory signs resolved, and after 24 h, skin changes resolved completely. Based on clinical signs and treatment success, the clinical suspicion of milk allergy causing anaphylactic reaction (type I hypersensitivity) was confirmed.(AU)

A alergia ao leite é a única doença auto-alérgica em bovinos. Caracteriza-se pela retenção e absorção do próprio leite, fazendo com que as proteínas do leite, principalmente a alfa caseína, tenham acesso à corrente sanguínea, resultando em uma hipersensibilidade do tipo I. O objetivo deste relato é descrever um caso de doença autoalérgica em bovinos. Relatamos o caso de uma fêmea bovina da raça Jersey, com quatro anos de idade, prenhe e pesando 400Kg. O animal estava em tratamento (oculto para revisão)e tinha histórico de reação alérgica aguda. Proprietário relatou aparecimento súbito de lesões na pele, salivação e dificuldade respiratória. Oanimal estava participando de uma exposição agrícola, e não estava sendo ordenhado para gerar ingurgitamento da glândula mamária (retenção de leite). No exame físico identificou-sepápulas e placas urticariformes multifocais, principalmente nas laterais e flancos direito e esquerdo, ausência de prurido, edema em regiões mucocutâneas das pálpebras superior e inferior dos olhos direito e esquerdo, edema submandibular, salivação excessiva, dispneia inspiratória com respiração de boca entreaberta, desconforto respiratório, apatia, tosse, taquicardia, taquipneia, hipomotilidade ruminal e aumento do volume da glândula mamária, caracterizando retenção de leite. A suspeita clínica foi alergia ao leite com reação anafilática (hipersensibilidade tipo I). O tratamento baseou-se na administração de prometazina (1mg/kg; IV) e ordenha completa imediata da glândula mamária. Após 12 h de tratamento, os sinais clínicos respiratórios foram resolvidos e, após 24 h, as alterações cutâneas desapareceram completamente. Com base nos sinais clínicos e no sucesso do tratamento, confirmou-sea suspeita de alergia ao leite causando reação anafilática (hipersensibilidade tipo I).(AU)

La alergia a la leche es la única enfermedad autoalérgica en el ganado bovino. Se caracteriza por la retención y absorción de la propia leche, provocando que las proteínas de la leche, especialmente la alfa caseína, tengan acceso al torrente sanguíneo, dando lugar a una hipersensibilidad tipo I. El objetivo de este reporte es describir un caso de enfermedad autoalérgica en bovinos. Presentamos el caso de una vaca Jersey hembra de cuatro años, gestante y de 400 kg de peso. El animal estaba siendo atendido en el (oculto para revision)y tenía antecedentes de reacción alérgica aguda. Propietario refirió aparición repentina de lesiones en la piel, salivación y dificultad respiratoria. Como el animal participaba en una exhibición agrícola, no estaba siendo ordeñado para generar congestión de la glándula mamaria (retención de leche). En examen físico en los que se observan pápulas y placas urticariales multifocales, principalmente en costados y flancos derecho e izquierdo, además de ausencia de prurito, edema en las regiones mucocutáneas de los párpados superior e inferior del lado derecho e ojos izquierdos, edema submandibular, salivación excesiva, disnea inspiratoria con respiración con la boca abierta, dificultad respiratoria, apatía, tos, taquicardia (frecuencia cardíaca 88 latidos/minuto), taquipnea (100 movimientos/minuto), normotermia (39,0°C), hipomotilidad ruminal (2 movimientos ruminales/5 minutos) y aumento de volumen de la glándula mamaria, caracterizando la retención de leche. La sospecha clínica fue alergia a la leche con reacción anafiláctica (hipersensibilidad tipo I). El tratamiento se basó en la administración de prometazina (1 mg/kg; IV) y ordeño completo inmediato de la glándula mamaria. Después de 12 h de tratamiento, los signos clínicos respiratorios se resolvieron y, después de 24 h, los cambios en la piel desaparecieron por completo. Sobre la base de los signos clínicos y el éxito del tratamiento, se confirmó la sospecha clínica de alergia a la leche que causaba una reacción anafiláctica (hipersensibilidad tipo I).(AU)

Induced corneal ulcers in cats - effects of 2% dorzolamide on epithelization time and on the expression of matrix metalloproteinase-9

Background: Topically administered 2% dorzolamide is among the most commonly used agents to lower IOP. As a complication of glaucoma, blind patients may develop corneal ulcers secondary to trauma. Nonetheless, in patients with a hypertensive or glaucomatous eye, in which the cornea has also been ulcerated, medical hypotensive therapy should not be discontinued. Therefore, the present study aimed to determine whether the instillation of a benzalkonium chloride (BAK)-preserved 2% dorzolamide alters corneal wound healing time and the levels of matrix metalloproteinases (MMP-9) in the tears of cats with experimentally induced corneal ulcers. Materials, Methods & Results: Sixteen cats (8/group) were randomly assigned to receive 40 µL of 2% dorzolamide (TG) or saline (CG) 3 times daily until corneal re-epithelialization. Experimental keratectomies were performed under general and topical anesthesia using an operating microscope. For this purpose, a millimitred trephine was calibrated and used to create a temporal paraxial corneal ulcer with a diameter of 6 mm and a depth of 200 µm. After corneal wounding, the ulcerated area, the healing time, blepharospasm, conjunctival hyperemia, and aqueous flare were compared between groups. Tears were collected at baseline and 24 and 48 h after keratectomy, and the total MMP-9 was quantified by ELISA. Data were assessed statistically using unpaired Student's t test, one-way, and two-way ANOVA followed by a Bonferroni post hoc test. Statistical significance was set at P < 0.05 for all analyses. The average time to achieve corneal wound healing did not differ between groups (P = 0.36) and was 65.50 ± 3.62 h in the CG and 71.00 ± 4.58 h in the TG. Twenty-four h after keratectomy, the ulcerated area in the CG was 3.34 mm2 larger than that observed in the TG (P = 0.04); the rest of the comparisons did not reach statistical significance at any time point between groups (P > 0.05). Higher blepharospasm scores were observed in cats of TG (P = 0.04). When compared with baseline of both groups, the levels of MMP-9 increased significantly at 24 and 48 h post-keratectomy (P < 0.001), but differences between groups were not observed at 24 and 48 h post-keratectomy (P > 0.05). Discussion: In cats, 9 mm axial corneal ulcers created by superficial debridement re-epithelize approximately 48 h postwounding. In the present study, re-epithelialization post keratectomy occurred within an average time of 68.25 h in most cats and in a delayed manner in one cat of the TG after 96 h. In the current study, the lesions in both groups healed without corneal scarring, pigmentation, or vascularization. Although BAC was present in all topical medications used in the present study, the authors attribute the higher scores of blepharospasm in the TG to the rheological characteristics and the pH of the dorzolamide ophthalmic solution. Indeed, the pH value of dorzolamide (5.58) may cause signs of irritation, as the tear film has an approximate pH of 7.6. Previous studies showed that ulcerated corneas presented significantly higher levels of MMP-9 in tears at the early stages (8 to 36 h) post-wounding. In the current study, the levels of this enzyme after wounding did not change significantly in the tears of cats treated with 2% dorzolamide when compared to the eyes in the control group. This study showed that the instillation of a BAC-preserved 2% dorzolamide ophthalmic solution did not impair the corneal wound healing time or the early expression of MMP-9 in the tears of cats with experimentally induced corneal ulcers. However, our results warrant further investigation in patients with ocular hypertension or glaucoma presenting concomitant naturally occurring corneal ulcers to certify our findings.

Toxicological effects of thimerosal on rat kidney: a histological and biochemical study / Efeitos toxicológicos do timerosal no rim de rato: um estudo histológico e bioquímico

Thimerosal is an organomercurial compound, which is used in the preparation of intramuscular immunoglobulin, antivenoms, tattoo inks, skin test antigens, nasal products, ophthalmic drops, and vaccines as a preservative. In most of animal species and humans, the kidney is one of the main sites for mercurial compounds deposition and target organs for toxicity. So, the current research was intended to assess the thimerosal induced nephrotoxicity in male rats. Twenty-four adult male albino rats were categorized into four groups. The first group was a control group. Rats of Group-II, Group-III, and Group-IV were administered with 0.5µg/kg, 10µg/kg, and 50µg/kg of thimerosal once a day, respectively. Thimerosal administration significantly decreased the activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), glutathione reductase (GR), glutathione (GSH), and protein content while increased the thiobarbituric acid reactive substances (TBARS) and hydrogen peroxide (H2O2) levels dose-dependently. Blood urea nitrogen (BUN), creatinine, urobilinogen, urinary proteins, kidney injury molecule-1 (KIM-1), and neutrophil gelatinase-associated lipocalin (NGAL) levels were substantially increased. In contrast, urinary albumin and creatinine clearance was reduced dose-dependently in thimerosal treated groups. The results demonstrated that thimerosal significantly increased the inflammation indicators including nuclear factor kappaB (NF-κB), tumor necrosis factor-α (TNF-α), Interleukin-1β (IL-1β), Interleukin-6 (IL-6) levels and cyclooxygenase-2 (COX-2) activities, DNA and histopathological damages dose-dependently. So, the present findings ascertained that thimerosal exerted nephrotoxicity in male albino rats.

O timerosal é um composto organomercurial, utilizado na preparação de imunoglobulina intramuscular, antivenenos, tintas de tatuagem, antígenos de teste cutâneo, produtos nasais, gotas oftálmicas e vacinas como conservante. Na maioria das espécies animais e nos humanos, o rim é um dos principais locais de deposição de compostos de mercúrio e órgãos-alvo de toxicidade. Assim, a presente pesquisa teve como objetivo avaliar a nefrotoxicidade induzida pelo timerosal em ratos machos. Vinte e quatro ratos albinos machos adultos foram categorizados em quatro grupos. O primeiro grupo era um grupo de controle. Ratos do Grupo II, Grupo III e Grupo IV receberam 0,5µg / kg, 10µg / kg e 50µg / kg de timerosal uma vez ao dia, respectivamente. A administração de timerosal diminuiu significativamente as atividades de catalase (CAT), superóxido dismutase (SOD), peroxidase (POD), glutationa redutase (GR), glutationa (GSH) e conteúdo de proteína, enquanto aumentou as substâncias reativas ao ácido tiobarbitúrico (TBARS) e peróxido de hidrogênio (H2O2) níveis dependentes da dose. Os níveis de nitrogênio ureico no sangue (BUN), creatinina, urobilinogênio, proteínas urinárias, molécula de lesão renal-1 (KIM-1) e lipocalina associada à gelatinase de neutrófilos (NGAL) aumentaram substancialmente. Em contraste, a albumina urinária e a depuração da creatinina foram reduzidas de forma dependente da dose nos grupos tratados com timerosal. Os resultados demonstraram que o timerosal aumentou significativamente os indicadores de inflamação, incluindo fator nuclear kappaB (NF-κB), fator de necrose tumoral-α (TNF-α), interleucina-1β (IL-1β), níveis de interleucina-6 (IL-6) e atividades da ciclooxigenase-2 (COX-2), DNA e danos histopatológicos dependentes da dose. Portanto, os presentes achados verificaram que o timerosal exerceu nefrotoxicidade em ratos albinos machos.

Analgesia perioperatória de gatas submetidas a ovariohisterectomia eletiva - uso preemptivo de carprofeno ou grapiprant / Perioperative analgesia in cats undergoing elective ovariohysterectomy - preemptive use of carprofen or grapiprant

Background: The nonsteroidal anti-inflammatory drugs (NSAIDs) exert their analgesic effect through peripheral inhibition of prostaglandin synthesis and a variety of other peripheral and central mechanisms. However, NSAIDs are associated with some adverse effects, mainly related to the gastrointestinal, renal, and hepatic systems, highlighting the need for research to develop safer drugs. Therefore, the aim of this study was to evaluate the efficacy of preoperative oral administration of carprofen or grapiprant in female cats submitted to elective ovariohysterectomy on the quality of perioperative analgesia and the need for hypnotic and analgesic drugs. Materials, Methods & Results: Thirty-three adult female cats were selected, without defined breed and healthy based on physical examination, routine laboratory analyses (complete blood count, total protein, Heinz body investigation and serum quantification of alanine transaminase [ALT], aspartate transaminase [AST], gamma glutamyl transpeptidase [GGT], alkaline phosphatase [ALP], urea, frutosamine, and glucose) and negative tests for feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV). After 3 days of adaptation, they were submitted to ovariohysterectomy by celiotomy and randomly allocated into 2 groups according to the preoperative drug used: GCAR [carprofen - 4 mg/kg, VO, 2 h before surgery; n = 11] and GGRA (grapiprant - 2 mg/kg IV, 2 h before surgery; n = 21]. The cats were pre-medicated with acepromazine 0.05 mg/ kg IV and later submitted to general anesthesia with propofol intravenously. Anesthesia was maintained with isoflurane in 100% oxygen. After anesthetic induction, a continuous infusion of remifentanil at a rate of 10 µg/kg/h was initiated. During the transanesthetic period, the parameters of heart rate; respiratory rate; systolic, mean, and diastolic arterial pressure using the oscillometric method; electrocardiogram; rectal temperature; partial pressure of CO2 at the end of expiration: and partial saturation of O2 in hemoglobin were continuously monitored. The evaluation of nociception was based on the changes in the aforementioned physiological parameters. The rate of remifentanil used did not change over time with the use of carprofen. However, animals that received grapiprant required a lower remifentanil dose at 20, 25, and 30 min during the procedure. The female cats that received carprofen showed an increase in mean heart rate at 30 min compared to that at 20 and 25 min. In the Grapiprant group, the heart rate at 35 min was higher only than that observed at 25 min. Discussion: The remifentanil rate did not differ between the groups, even between the times for GCAR. However, the remifentanil rate was lower from 20 min of the procedure for GGRA. This decrease may be related to a decrease in the need for anesthetics and analgesics by decreasing temperature, which causes decreases in metabolism and surgical stimulation. The increase in systolic, mean, diastolic, and heart rate arterial pressure parameters observed in both treatments after 15 min of anesthesia is related to the nociceptive stimulus resulting from traction and ligation of the ovarian pedicles and maneuvers for exteriorization of the uterus. These are considered the moments of greater surgical stimulus during ovariohysterectomy, evidenced by the greater release of cortisol and increase in physiological parameters. The results of this study show that the administration of carprofen or grapiprant was clinically similar when used preemptively for perioperative analgesia in cats submitted to elective ovariohysterectomy.