Thecoma in an elderly crioulo mare

Background: Ovarian enlargement in mares can either be a physiological or pathological condition associated with neoplastic or non-neoplastic causes. Neoplasia is more prominent in elderly than young horses. Granulosa cell tumors are the most common neoplastic cause of ovarian enlargement, and thecoma is the rarest. Thecoma is a benign ovarian stromal neoplasia originating from the sexual cords of the embryonic gonad and can affect the production of steroid hormones, leading to changes in behavior and the estrous cycle. If only one ovary is affected, after unilateral ovariectomy, usually the mare can still be used for reproductive purposes. Herein, we report a case of ovarian thecoma in an elderly Crioulo mare. Case: A 15-year-old Crioulo mare was evaluated due to progressive weight loss. The mare presented a low body score, and on physical examination, all parameters were within reference limits. Hematological examination revealed anemia, hyperfibrinogenemia, and neutrophilia. Serum biochemistry examination were within the physiological limits. Rectal palpation revealed an increase in volume in the left ovary (4.3 × 2.9 × 3 cm), smooth consistency and no associated pain in either of the ovaries. Rectal ultrasonography revealed a heterogeneous pattern, suggesting a neoplasm. As a treatment modality and for definitive diagnosis, the mare underwent a unilateral flank ovariectomy laparoscopically in the quadrupedal position. On histopathological evaluation of the left ovary, the sample showed cysts associated with proliferating spindle cells arranged in a solid arrangement, occasionally forming eddies, in addition to hemosiderosis, with clear cytoplasm and lipid vacuoles. Macroscopically, the ovary presented with a regular surface, smooth to firm consistency, a homogeneous brownish appearance with blackened foci, and brownish fluid-filled cysts. These findings confirmed thecoma of the left ovary in the mare. Discussion: Ovarian enlargement in mares can be associated to the presence of neoplasia. Clinical signs of ovarian neoplasms can be nonspecific; therefore, to achieve a definitive diagnosis, it is imperative to evaluate and compare the patient's history, physical examination, and results of complementary examinations. Excessive production of hormones lead to behavioral changes in mares with ovarian tumors, such as masculinized or aggressive behavior, and anestrus persistence. Thecomas may or may not interfere with steroid production and levels. On ultrasound examination, ovarian neoplasms may present similar echogenicity patterns. Histopathological evaluation is the best diagnostic approach after an ovariectomy. The prognosis of ovariectomy is considerably successful with the mare regaining reproductive health, although some mares may become infertile, with a small, inactive contralateral ovary. Thecoma is a rare neoplasm in horses; however, owing to its potential to cause altered reproductive functions, it should be considered as a differential diagnosis in mares with increased ovarian volume.

Biópsia endometrial na égua: importância no diagnóstico da subfertilidade e principais achados / Endometrial biopsy in the mare: importance in the diagnosis of subfertility and main findings

A biópsia endometrial na égua é uma técnica simples e bastante antiga. É uma importante ferramenta para o diagnóstico de patologia no endométrio nesta espécie utilizada para determinar o estágio do ciclo estral, para detectar a presença de inflamações agudas ou crônicas e para determina a possibilidade do útero levar a gestação a termo. Mais recentemente outras alterações tais como a má-diferenciação e as angiopatias, também estão sendo avaliadas em amostras de biópsia endometrial em éguas, uma vez que também podem interferir no prognóstico reprodutivo destas fêmeas. Esta revisão tem por objetivo descrever a avaliação da biópsia endometrial e as principais alterações que ocorrem no endométrio das éguas.(AU)

Endometrial biopsy in the mare is a simple and quite old technique. It is an important tool for the diagnosis of pathology in the endometrium in this species, used to determine the stage of the estrous cycle, to detect the presence of acute or chronic inflammation and to determine the possibility of the uterus carrying the pregnancy to term. More recently, other alterations, such as poor differentiation and angiopathies, are also being evaluated in endometrial biopsy samples in mares, since they may also interfere with the reproductive prognosis of these females. This review aims to describe the evaluation of the endometrial biopsy and the main changes that occur in the endometrium of mares.(AU)

Luz artificial na reprodução equina ­ Revisão / Artificial lighting in the equine reproduction ­ A review

Os equinos são animais poliéstricos sazonais, fotoperiódico positivos, apresentando ciclos reprodutivos em estações definidas do ano, primavera e verão, e estações com ausência ou baixa atividade reprodutiva, outono e inverno. A necessidade da maximização do período reprodutivo, bem como a definição de um ano hípico iniciando em julho, no hemisfério sul (para algumas raças como o Puro Sangue de Corrida e o Quarto de milha) levou ao desenvolvimento de técnicas para diminuir o tempo que essas éguas permanecem em anestro reprodutivo. Uma das técnicas mais utilizadas destaca-se o uso de iluminação artificial. Consistindo no fornecimento de luz branca incandescente ou led azul ou branco em baias, piquetes ou por meio de máscaras, a fim de simular os efeitos de dias longos no organismo assim liberar o eixo hipotalâmico-hipofisário-gonadal. Para estimular o retorno antecipado à ciclicidade, a égua deve ser exposta a um estímulo de 14 a 15 horas de luz diárias, totalizando 9 a 10 horas de escuridão, ou receber um estímulo de 1 hora de luz, aproximadamente 9 horas após o anoitecer. É fundamental que a égua esteja com escore corporal adequado e alguns cuidados com o fornecimento da luz artificial sejam atendidos, como a continuidade no fornecimento para o sucesso da técnica.(AU)

Horses are seasonally breeding animals which means that they natural breed in specific seasons (Spring, and Summer), with non or low reproductive activity on the other seasons (Winter and Fall). The development of techniques to shorten the anoestrus can be necessary to anticipate the breeding season, starting in July in the Southern Hemisphere, for some breeds such as Thoroughbred and Quarter Horses. The most common technique is the use of artificial lighting with incandescent, or blue/white led light, applied in stalls, paddocks or with a light mask to mimic the effects of a long day and release the hipotalamic-hipophysarie-gonadal axis. To stimulate an early return to cyclicity, the mare should be exposed to a 14-to-15-hour light stimulus daily, totalling 9 to 10 hours of darkness, or receive a 1-hour light stimulus, approximately 9 hours after dusk. It is fundamental that the mare has an adequate body condition score and some awareness with the provision of artificial light are considered, such as the continuity of the light program for the success of the technique.(AU)

Mammal prevalence after the fire catastrophe in northeastern Pantanal, Brazil

Fire might occur under natural conditions in the Pantanal of Brazil; however, with climate change, severe periods of drought potentiated the devasting fires in 2020, resulting in substantial wildlife loss. Considering that mammal communities are strongly affected by fire and habitat alterations, the aim of this study was to evaluate possible differences in mammal diversity and the number of records before and one year after the fire in one region of the Pantanal of Mato Grosso, Brazil (Parque SESC Baía das Pedras - PSBP). The data collection was performed using camera trapping between 2015 and 2017 and 2021, together with visual field observations in PSBP. We observed that the mammal assemblage composition was similar before and one year after the fire. Four species were more or less frequent in burned areas than in unburned ones. Since the fire was controlled in this area, avoiding its total destruction, and the species that fled from the surrounding areas, which were completely burnt, might be using PSBP as a refuge while the vegetation recovers elsewhere. Therefore, the PSBP might have contributed to protecting mammal species after the fire and maintaining and conserving biodiversity on a regional scale in the Pantanal of Mato Grosso, Brazil.

The equine endometrium and its multiple connections, interrelationships and clinical effects in aged mares

Mares, which make up 90% of animals requiring reproductive assistance in commercial equine production systems, are subjected to strong breeding and reproductive efficiency pressure throughout their lives, but the effects of aging on overall and reproductive systems in particular; it often means that we are forcing the natural homeostasis mechanisms under pressure over time. The objective of this brief communication is to summarize some concepts related to the role and importance of equine endometrium, particularly in old mares, the most challenged category to achieve productive and efficiency goals established by humans. Endometritis is one of the most frequent and recurrent pathologies in equine gynecology and is responsible for enormous economic losses in the industry. Alterations of the microbiota, molecular signals and uterine endocrine microenvironment can lead to infertility, but degenerative phenomena associated with age and parity can lead to endometriosis altering maternal recognition of pregnancy and pregnancy maintenance. Maintaining horses in an adequate nutrition status and environment is essential to achieve pregnancy but also to obtain healthy offspring.(AU)

Amphibian Diversity: Where everything starts to flood, Cáceres Municipality, North Pantanal, Central­West Brazil

Faunal inventories contribute to our understanding of regional diversity, and are fundamental for policy and de-cision-making regarding the management and conservation of large natural areas. This study aimed to inventory and com-pile information on amphibian species occurring in the North Pantanal region, in the municipal limits of Cáceres, Mato Grosso, Brazil. We used three methods to inventory amphibian species: (1) fieldwork, (2) analysis of specimens deposited in scientif-ic collections, and (3) literature reviews. We registered 49 amphibian species in Cáceres. Of them, 48 species belonged to the Anura order and were distributed across eight families and 20 genera, and one species belonged to the Gymnophiona order (Siphonops paulensis). The families Leptodactylidae (20 spp.) and Hylidae (17 spp.) were dominant in terms of richness, ac-counting for 75.5% of all species found in Cáceres. The remaining families had between four and one species each. The mu-nicipality is strongly influenced by non-forested formations (e.g., the Cerrado and Pantanal) and presents a high species rich-ness for a non-forested location in Brazil. Our findings highlight Cáceres as one of the richest areas in amphibian species in the North Pantanal region, expanding our knowledge regarding frog diversity. This study provides a foundation for future conser-vation strategies and additional assessments of amphibian species in light of potential population declines and other emerg-ing threats.(AU)

Physical attributes and yield of wheat seeds originated from plants under temporary flooding of the soil / Atributos físicos e rendimento de sementes de trigo originadas de plantas sob alagamento temporário do solo

The aim of this work was to evaluate whether there is any influence of temporary soil flooding on the agronomic performance and yield of wheat varieties. The experimental design was completely random blocks, in an 11x2 factorial scheme (eleven varieties and two soil water conditions), with four replications. Stress was imposed in the stage prior to plant anthesis. The assessments were made in two seasons (2016/2017) in relation to the number of spikelets in the main ear, number of seeds in the main ear, mass of seeds in the main ear, number of seeds in the tiller ear, mass of seeds in the tiller ear and yield in the useful area. The physical attributes number of seeds, seed mass and wheat seed yield, according to variety and agricultural year, are negatively affected by the temporary flooding (12 days) of the soil. There is influence of temporary soil flooding on the agronomic performance and yield of wheat varieties. The varieties TBio Toruk, TBio Sinuelo, TBio Sintonia, and CD151 are superior in soil flooding condition for the number of spikelets, number of seeds end for the seed mass attribute. In the soil at field capacity, the seeds produced have higher yield compared to those produced under flooding period, reaching about 309.2 Kg more for the variety TBio Sinuelo, 262 Kg for TBio Toruk and 114 Kg in CD1303.

O trabalho teve como objetivo avaliar se há influência do alagamento temporário do solo no desempenho agronômico e no rendimento de cultivares de trigo. O delineamento experimental foi em blocos ao acaso, em esquema fatorial 11x2 (onze cultivares e duas condições hídricas do solo), com quatro repetições. O estresse foi o imposto no estádio antecedente a antese das plantas. Foi realizada a avaliação por dois anos (2016/2017) do número de espigueta da espiga principal, número de sementes da espiga principal, massa de sementes da espiga principal, número de sementes da espiga do perfilho, massa de sementes da espiga do perfilho e rendimento da área útil. Os atributos físicos número de sementes, massa de sementes e o rendimento de sementes de trigo, conforme a cultivar e ano agrícola, são afetados negativamente pelo alagamento temporário (12 dias) do solo. Há influência do alagamento temporário do solo no desempenho agronômico e na produtividade das variedades de trigo. As variedades TBio Toruk, TBio Sinuelo, TBio Sintonia e CD151 são superiores na condição de alagamento do solo para o número de espiguetas, número de sementes e para o atributo massa de sementes. Na condição de capacidade de campo, as sementes produzidas apresentam maior rendimento, atingindo cerca de 309,2 Kg a mais para a cultivar TBio Sinuelo, 262 Kg para a TBio Toruk e 114 Kg na CD1303 em comparação àquelas produzidas sob o período de alagamento imposto.

Comparative growth of two mussels farmed in the macrotidal mangroves of the Amazon / Crescimento comparativo de dois mexilhões cultivados em manguezais de macromarés da Amazônia

The present study evaluated the growth of the species Mytella guyanensis and Mytella strigata on ropes suspended in Amazon Macrotidal Mangrove Coast. The mussels were farmed at a density of 840 ind. m-1 of rope, with the same shell height (mm) and live weight (g) for both species. The experiment was entirely randomized, with two treatments and 15 repetitions. Significant differences were reported regarding the growth (shell height and live weight) between the species (P>0.05). The daily growth rate was greater for M. guyanensis than for M. strigata. At the end of the cultivation cycle, only 451 ± 46 (59.9%) of M. guyanensis individuals reached commercial size per meter of rope compared to 670 ± 73 (89.3%) of M. strigata individuals. Survival rates were similar. Salinity and temperature increased progressively throughout the experiment with the reduction in rainfall and were within the range considered ideal for these species. In conclusion, the farming of both species of mussels was viable under estuarine conditions influenced by macrotides, with satisfactory survival rates and daily growth (shell height and weight).

O presente trabalho avaliou o crescimento das espécies Mytella guyanensis e Mytella strigata em cordas suspensas na Costa de Manguezais de Macromarés da Amazônia. Os mexilhões foram cultivados na densidade de 840 ind. m-1 de corda, com a mesma altura de valva (mm) e peso vivo (g) para ambas as espécies. O experimento ocorreu inteiramente casualizado, com dois tratamentos e 15 repetições. Foram encontradas diferenças significativas quanto ao crescimento (altura da concha e peso vivo) entre as espécies (P>0,05). A taxa de crescimento diário foi maior para a M. guyanensis do que para M. strigata. Ao final do ciclo de cultivo, apenas 451 ± 46 (59,9%) indivíduos de M. guyanensis atingiram o tamanho comercial por metro de corda comparado a 670 ± 73 (89,3%) indivíduos de M. strigata. As taxas de sobrevivência foram semelhantes. A salinidade e a temperatura aumentaram progressivamente ao longo do experimento com a redução das chuvas e ficaram dentro da faixa considerada ideal para essas espécies. Em conclusão, o cultivo de ambas as espécies de mexilhões foi viável em condições estuarinas influenciadas pelas macromarés, com taxas de sobrevivência e crescimento diário satisfatórios (altura de valva e peso).

Nodular lymphocytic conjunctivitis in a horse - case report / Conjuntivite linfocítica nodular em equino - relato de caso

The case of an equine with nodular lymphocytic conjunctivitis is described. A 14-year-old crossbred mare was seen presenting with development of a mass in the nasal angle of the right eye, behind the third eyelid. The tutor reported slow growth over 4 years, always accompanied by epiphora, and that no treatment had been performed prior to consultation. The histopathological and immunohistochemical results found a nodular, subepithelial structure, composed predominantly of densely packed small lymphocytes. Through the exams, associated with studies with monoclonal anti B lymphocyte antibodies and polyclonal anti T lymphocyte antibodies, the diagnosis of nodular lymphocytic conjunctivitis was reached. Only clinical pharmacological treatment was chosen, based on the use of topical and intralesional hydrocortisone acetate. After one month of treatment the mass completely disappeared without sequelae.

Descreve-se o caso de um equino com conjuntivite linfocítica nodular. Uma égua, mestiça, de 14 anos, foi atendida apresentando desenvolvimento de uma massa no ângulo nasal do olho direito, atrás da terceira pálpebra. O tutor relatou um crescimento lento durante 4 anos, sempre acompanhado de epífora, para o qual não foi realizado tratamento prévio à consulta. Os resultados histopatológico e imuno-histoquímico constataram uma estrutura nodular e subepitelial, composta predominantemente por linfocitos pequenos densamente agrupados. Por meio dos exames, associados a estudos com anticorpos monoclonais antilinfocitos B e anticorpos policlonais antilinfocitos T, chegou-se ao diagnóstico de conjuntivite linfocítica nodular. Optou-se apenas pelo tratamento clínico farmacológico, baseando-se na utilização de acetato de hidrocortisona tópica e intralesional. Após um mês de tratamento, a massa involuiu por completo, sem deixar sequelas.

The monodominance of Acuri in Pantanal formations: allelochemical effects of its leaves and the presence of Acurizais / A monodominância do Acuri em formações do Pantanal: efeitos aleloquímicos de suas folhas e a presença de acurizais

The presence of monodominant vegetative formations almost exclusively composed of Acuri palm trees (Attalea phalerata) stands out in some regions of the Pantanal Sul-Mato-Grossense. These formations are generally associated with anthropic, edaphic and/or hydrological factors. However, little is known about the effect of allelopathy on the formation and maintenance of these areas. Herein, we investigated the chemical composition of A. phalerata aqueous leaf extract and the potential allelopathic effects on germination and growth of target L. sativa, L. esculentum and S. obtusifolia species. Thus, extracts at concentrations of 0, 2.5, 5, 10, 15, and 20% were used for germination and growth bioassays with a completely randomised design in a germination chamber and greenhouse. The results showed that the A. phalerata extracts negatively affected the germination speed index and mean germination time of the target species and positively affected seedling length under controlled conditions and were also stimulated in the greenhouse. Thus, the formation of Acurizals can be related to the presence of secondary metabolites in the leaves, in addition to other environmental factors.

No Pantanal Sul-Mato-Grossense se destaca, em determinadas regiões, a presença de formações vegetacionais monodominantes, compostas quase que exclusivamente por uma espécie de palmeira, o Acuri (Attalea phalerata). Normalmente estas formações estão associadas a fatores antrópicos, edáficos e/ou hidrológicos. Porém pouco se sabe sobre o efeito da alelopatia na formação e manutenção destas áreas. Desta maneira, objetivou-se estudar a composição química dos extratos aquosos das folhas de A. phalerata e seus possíveis efeitos alelopáticos na germinação e crescimento das espécies-alvo, alface, tomate e fedegoso. Para os bioensaios de germinação e crescimento, foram utilizados extratos nas concentrações de 0, 2,5, 5, 10, 15 e 20%, com delineamento inteiramente casualizado, em câmara de germinação e casa de vegetação. Os resultados demostraram que em condições controladas (câmara de germinação), os extratos de A. phalerata afetaram negativamente o índice de velocidade de germinação e o tempo médio de geminação das espécies-alvo e positivamente, o comprimento das plântulas, que também foram estimuladas em casa de vegetação, indicando a presença de fitormônios. Desta maneira, a formação de Acurizais pode estar relacionada à presença de metabolitos secundários nas folhas, além de outros fatores ambientais.

Manejo das principais anormalidades gestacionais em éguas / Management of the main gestational abnormalities in mares

A produção de potros é o objetivo final de todas as atividades voltadas para a reprodução equina. Para tanto, o diagnóstico de anormalidades gestacionais deve ser realizado precocemente para que a instalação da terapêutica adequada seja garantida, visando manter a sobrevivência da égua e o nascimento de indivíduos vivos e saudáveis. Aqui encontram-se descritos os principais achados relacionados às anormalidades gestacionais de éguas mais frequentemente encontradas em atendimentos realizados a campo e nas rotinas hospitalares. São elas: gestação gemelar, placentite, separação prematura da placenta, torção uterina, hidropsias e ruptura de tendão pré-púbico. O objetivo é caracterizar os sinais clínicos, métodos diagnósticos, tratamento e prognóstico dessas enfermidades. A compreensão desses aspectos é essencial para o desenvolvimento de estratégias de prevenção e gestão das intercorrências obstétricas, visando reduzir seu impacto na criação de equinos.(AU)

The primary objective of equine reproduction is to produce healthy foals. To achieve this, it is crucial to diagnose gestational abnormalities at an early stage and administer appropriate therapy. This will increase the chances of survival for the mare and the birth of live foals. Here we outlines the most frequently observed gestational abnormalities in mares during field visits and hospital routines. The following abnormalities discussed are: twin pregnancy, placenta problems, premature separation of the placenta, uterine torsion, hydrops, and rupture of the prepubic tendon. The objective is to characterize the clinical signs, diagnostic methods, treatment, and prognosis for each of these conditions. It is essential to understand these aspects of gestational abnormalities to develop effective prevention and management strategies for obstetric complications. aiming to reduce their impact on equine breeding.(AU)

A arte de inseminar éguas com sêmen congelado / The art of inseminating mares with frozen semen

Nos últimos quinze anos, a equideocultura foi uma atividade em destaque com significativo crescimento no Brasil e no mundo. O Brasil é o segundo país no mundo que mais utiliza transporte de sêmen equino, ficando atrás apenas dos Estados Unidos e a utilização do sêmen congelado no país vem expandido a cada dia. O índice de prenhez por ciclo, com sêmen equino congelado é variável e oscila entre 25 e 40%. Adicionalmente, sabe-se que o sêmen de alguns garanhões apresenta baixa viabilidade após o descongelamento. A primeira prenhez obtida com sêmen equino congelado foi relatada há cinco décadas. Segundo Cazalez, 2020, é muito difícil recomendar uma dose inseminante padrão para sêmen congelado em equinos. A maioria dos estudos científicos não consegue controlar o efeito de outros fatores "de confusão" como método de processo, fator égua, fator garanhão, técnica de inseminação etc., tornando difícil uma comparação crítica dos mesmos. Rigby et al. (2001) obtiveram taxas de prenhez similares ao se comparar a inseminação artificial profunda em corno uterino com endoscópio e pipeta flexível.(AU)

In the last fifteen years, equine breeding has been a prominent activity with significant growth in Brazil and in the world. Brazil is the second country in the world that most uses equine semen transport, second only to the United States, and the use of frozen semen in the country is expanding every day. The pregnancy rate per cycle with frozen equine semen is variable and ranges from 25 to 40%. Additionally, it is known that the semen of some stallions has low viability after thawing. The first pregnancy obtained with frozen equine semen was reported five decades ago. According to Cazalez, 2020, it is very difficult to recommend a standard insemination dose for frozen semen in horses. Most scientific studies cannot control for the effect of other "confounding" factors such as processing method, mare factor, stallion factor, insemination technique, etc., making it difficult to critically compare them. Rigby et al. (2001) obtained similar pregnancy rates when comparing deep artificial insemination in the uterine horn with an endoscope and flexible pipette.(AU)

Medium and large-sized mammals of a private protected wetland in the Cerrado-Amazon biological corridor, Brazil / Mamíferos de médio e grande porte em uma área alagável protegida no corredor biológico Cerrado-Amazônia, Brasil

Abstract Brazil is the world's richest country in biodiversity, including mammal species. In the Brazilian Cerrado biome, mammalian diversity is vast, with about 251 species, 32 of them are endemic and 22 listed as threatened species. In this work, we investigated species diversity of medium- and large-sized mammals in the private protected area RPPN Pontal do Jaburu (RPPN-PJ) and its surroundings, which is a flooded area located in an important biological corridor in the Cerrado-Amazon ecotone zone, a priority area for biodiversity conservation in Brazil. We used camera-trapping, active search (night and day), and track survey during dry season (Apr - Aug 2016). We recorded 29 mammal species, being the Carnivora order the most representative with 11 species. Regarding threat status, 35.7% of the recorded species were listed as threatened in Brazil and 32.1% worldwide. We highlight the high relative frequency of threatened species records such as Tapirus terrestris, Panthera onca, Blastocerus dichotomus, Pteronura brasiliensis, Priodontes maximus, and other, as well as the presence of the newly described aquatic mammal species Inia araguaiaensis. We stress the importance of RPPN-PJ and its surroundings for mammal conservation, which include complex habitats (wetlands) located in an important ecotone zone.

Resumo O Brasil é o país mais rico em biodiversidade no mundo, incluindo espécies de mamíferos. No bioma Cerrado, a diversidade de mamíferos é enorme, com cerca de 251 espécies, sendo 32 delas endêmicas e 22 listadas como ameaçadas de extinção. Neste estudo, investigamos a diversidade de espécies de mamíferos de médio e grande porte da RPPN Pontal do Jaburu (RPPN-PJ) e seu entorno, que é uma floresta de inundação localizada em um importante corredor biológico na zona de ecótono Cerrado-Amazonia, uma área prioritária para conservação da biodiversidade no Brasil. Os dados foram coletados por armadilhas fotográficas, busca ativa (noturna e diurna) e identificação de pegadas durante a estação seca (abril - agosto de 2016). Registramos um grande número de espécies de mamíferos (n = 29), sendo a ordem carnívora a mais representativa com 11 espécies. Com relação ao status de ameaça, 34,5% das espécies registradas foram listadas como ameaçadas na lista vermelha do Brasil e 20,7% na lista vermelha da IUCN. Destacamos a alta frequência relativa de registros de espécies ameaçadas como Tapirus terrestris, Panthera onca, Blastocerus dichotomus, Pteronura brasiliensis, Priodontes maximus, bem como a presença da recém descrita espécie de mamífero aquático Inia araguaiaensis. Nós discutimos a importância da RPPN-PJ e seus arredores para a conservação de espécies de mamíferos, onde inclui habitats complexos (áreas de inundação) localizados em uma importante zona de ecótono.. Os resultados reforçam a relevância desta área para a conservação de mamíferos.

Hematological, serum biochemical results, and treatment approach of an Arabian mare with squamous cell carcinoma of the vulva - case report / Resultados hematológicos, bioquímicos séricos e abordagem de tratamento de uma égua Árabe com carcinoma escamoso de células da vulva - relato de caso

A 20-year-old Arabian mare, was brought to the obstetrics and gynecology clinic with the complaint of an unknown wound in the genital area. Although the mare's general condition was good, examination of the genital area revealed a wide-open wound in the vulva and posterior vagina, characterized by fibrotic growth. The biopsy sample was sent to the pathology laboratory. A diagnosis of squamous cell carcinoma (SCC) was made after histopathological examination. Blood samples for serum biochemistry and hematological tests were collected from the jugular vein. Hematological and serum biochemical findings are generally used to diagnose diseases in animals and humans. Hematological and serum biochemical findings are important with genital SCC. Therefore, the findings of these values are given in this case report.

Uma égua de 20 anos (raça árabe), foi levada à clínica de obstetrícia e ginecologia com a queixa de uma ferida desconhecida na área genital. Embora o estado geral da égua fosse bom, o exame da área genital revelou uma ferida amplamente aberta na vulva e na vagina posterior, caracterizada pelo crescimento fibrótico. A amostra da biópsia foi enviada para o laboratório de patologia. Um diagnóstico de carcinoma espinocelular (CEC) foi feito como resultado do exame histopatológico. Amostras de sangue para bioquímica sérica e testes hematológicos foram coletadas da veia jugular. Os resultados hematológicos e bioquímicos séricos são geralmente usados para diagnosticar doenças em animais e seres humanos. Os achados hematológicos e bioquímicos séricos são importantes com o SCC genital. Portanto, as descobertas destes valores são dadas neste relatório de caso.

Metastatic vulvar squamous cell carcinoma in a mare

Background: Squamous cell carcinoma (SCC) is a common cutaneous neoplasm in horses, which mainly affects the external genitalia, oral cavity, and periocular region. The development of SCC metastases is rare in these animals, and the most common occurrence is a marked local infiltration. Exposure to ultraviolet rays and skin depigmentation are the main etiological factors of SCC. Definitive diagnosis of the neoplasm is performed through histological examination of lesions. The present report describes the clinical, anatomopathological, and immunohistochemical (IHC) aspects of a case of metastatic SCC, with the vulva as the primary site in a mare. Case: A 17-year-old mare, mixed breed, was referred to the Veterinary Hospital of the Universidade de Passo Fundo (UPF), with history of areas of depigmented skin (pinto coat), and clinical history of anorexia, frequent episodes of colic, and recumbency. During clinical evaluation, heart rate (68 bpm), rectal temperature (38.4ºC), and respiratory rate (48 mpm) were elevated, and the oral mucosa was pale. The mare also had an ulcerated tumor mass involving the vulva, which extended to the inguinal region and involved the mammary gland. A cytological aspirate of the vulvar tumor was performed, in which no neoplastic cells were found. Next, a biopsy of 2 distinct areas of the vulva was performed. The material was sent for anatomopathological examination, which showed markedly pleomorphic malignant squamous cells, with individual keratinization and high mitotic index, organized in trabeculae with rare keratin pearl-like formations. The exam allowed the diagnosis of SCC Grade II. Due to the poor prognosis and high cost of treatment, the owner consented to euthanasia and necropsy examination. During necropsy, the vulvar tumor mass was grayish, firm to hard, infiltrative, and had friable areas. Tumor foci suggestive of metastasis were also observed in inguinal, mesenteric, mediastinal and renal lymph nodes, adrenal glands, lung, pericardium, medullary canal, intercostal muscles, right 15th rib, and tissue surrounding the azygos vein. Samples from all organs were collected for anatomopathological examination. Diagnosis of metastatic vulvar SCC was confirmed through histological and IHC studies, which evaluated the expression of cytokeratins (AE1/ AE3), as well as the proliferative activity of neoplastic cells through the PCNA marker. Discussion: The diagnosis of metastatic SCC was obtained through the observed clinical, necroscopic, histological, and IHC characteristics. This neoplasm usually appears in depigmented regions exposed to ultraviolet light, and older animals are more likely to be affected. The mare in the present case had areas of depigmented skin (pinto coat). The animal was kept in a paddock outdoor and exposed to constant solar radiation. Although rare in horses, manifestation of anorexia, progressive weight loss, and frequent colic episodes and recumbency may be closely related to the multiple sites of metastasis in the present case. The main histological findings of the neoplasm were the dense proliferation of malignant squamous cells with individual keratinization, arranged in a trabecular pattern and with rare formations of keratin pearls, in line with previous studies. During IHC evaluation, the neoplastic cells showed expression of cytokeratins (AE1/AE3), as well as high proliferative activity evidenced by the PCNA marker. Given this background, the present report describes the clinical, anatomopathological, and IHC aspects of a case of metastatic SCC with a primary site in the vulva of a mare.

Sinonasal multilobular tumor of bone in a young mare: case report / Tumor ósseo multilobular sinonasal em uma égua jovem: relato de caso

A two-year old mare was referred to the veterinary hospital because of an increase in the volume of the head on the frontal and left side as well as dyspnoea. Radiographic examination showed an osseous radiopaque spherical mass, with a granular aspect, affecting the left frontal, maxillary, and nasal bones. A frontal sinusotomy was performed, and the frontal sinus was found to be totally obliterated by a granular, yellowish, and hard mass containing multiple spicules. Histologically, it was a multilobulated osseous neoplasia diagnosed as a multilobular osseous tumour. Because of poor prognosis, the animal was euthanized. Necropsy showed that the left nasal cavity was totally effaced as were the nasal sinuses and that the nasal turbinate disappeared because of the hard, yellowish, and granular mass measuring 20 × 14 × 14 cm that surrounded the molar teeth and extended to the cribriform plate. This tumour, which was described only once in horses, is more frequently observed in older dogs' skulls, and must be considered as a differential diagnosis in horses with sinonasal diseases.

Uma égua de dois anos de idade foi encaminhada ao Hospital Veterinário devido a um aumento de volume nas regiões frontal e lateral esquerdas da cabeça, associado à dispneia. Exame radiográfico demonstrou a existência de uma massa esférica radiopaca de aspecto granular afetando os ossos frontal, maxilar e nasal esquerdos. Sinusotomia frontal foi realizada e o seio frontal era totalmente obliterado por uma massa amarelada, granular e dura, com múltiplas espículas. Histologicamente, era uma neoplasia óssea multilobulada, diagnosticada como tumor ósseo multilobular. Devido ao prognóstico ruim, a égua foi eutanasiada. Durante a necropsia, observou-se desaparecimento dos turbinados nasais assim como obliteração total da cavidade nasal esquerda e dos seios nasais por uma massa dura, amarelada e granular, de 20x14x14cm, que circundava os dentes molares e estendia-se à placa cribriforme. Esse tumor, que foi descrito apenas uma vez em equinos, é mais frequente no crânio de cães idosos e deve ser considerado como diagnóstico diferencial em equinos com doenças sinonasais.

Spatio-temporal variability in the Cladocera assemblage of a subtropical hypersaline lagoon

Abstract Cladocera represent an important zooplankton group because of their seasonal prominence in terms of abundance and their contribution in controlling primary production (phytoplankton). On a global scale, there are few studies on Cladocera in hypersaline environments. The present work aims to evaluate the spatio-temporal variation of the Cladocera assemblage across a salinity gradient in the habitats of the Araruama Lagoon. Samples were collected in random months over a period of four years at 12 fixed stations in the Araruama Lagoon using a WP2 plankton net equipped with a flow meter. Our results do not reveal significant influence of the tide and seasonal variation as factors affecting the Cladocera assemblage. Five Cladocera species were found in the Araruama Lagoon, only in stations 11 and 12 where they reached an average of 1,799 ± 3,103 ind. m-3. The mean of the Shannon Diversity Index was 0.45 ± 0.2. The species that stood out in terms of frequency and abundance were: Penilia avirostris (frequency of occurrence: 71%), followed by Pseudevadne tergestina (41%). The same species also stood out in terms of relative abundance, Penilia avirostris (87%) and Pseudevadne tergestina (11%). The absence of Cladocera in the innermost parts of the lagoon suggests that their entrance to these locations is possibly inhibited by the salinity and temperature gradient of the lagoon, being the main factors influencing the dynamics of the Cladocera assemblages.

Resumo Os cladóceros representam um importante grupo de zooplâncton, devido ao seu destaque sazonal em termos de abundância e à sua contribuição no controlo da produção primária (fitoplâncton). Em escala global, há poucos estudos com Cladocera em ambientes hipersalinos. O presente trabalho tem como objetivo avaliar a variação espaço-temporal da assembleia de Cladocera em um gradiente de salinidades da Lagoa de Araruama. As amostras foram coletadas em meses aleatórios, durante um período de quatro anos, em 12 estações fixas na Lagoa de Araruama, utilizando uma rede de plâncton WP2 equipada com um fluxômetro. Os nossos resultados não revelam uma influência significativa da maré e da variação sazonal como fatores que afetam a assembleia de Cladocera. Cinco espécies de Cladocera foram encontradas na Lagoa de Araruama. A assembleia apresentou maior densidade absoluta estações 12 e 11, atingindo uma a média de 1.799 ± 3.103 inds-3. A média do Índice de Diversidade de Shannon foi de 0,45 ± 0,2. As espécies que se destacaram em termos de frequência e abundância foram: Penilia avirostris (frequência de ocorrência: 71%), seguida de Pseudevadne tergestina (41%). As mesmas espécies também se destacaram em termos de abundância relativa Penilia avirostris (87%) e Pseudevadne tergestina (11%). A ausência de Cladocera nas partes mais interiores da lagoa sugere que a sua entrada nestes locais é possivelmente inibida pela salinidade e gradiente de temperatura da lagoa, sendo os principais fatores que influenciam a dinâmica da assembleia de Cladocera.

Coastal wetlands of the north of Peru: preservation progress

Wetlands provide several ecological, biological, and environmental benefits, including their role in the hydrological cycle, especially in coastal areas. Coastal wetlands are affected by anthropogenic threats, as these are in constant synergy with local populations and agricultural expansion, which is one of the main factors causing their depletion. In addition, the ravages of climate change accentuate their vulnerability, which may lead to their irreparable loss. In this regard, the objective of this workwas to identify the coastal wetlands of northern Peru and in order to verify the progress made in their conservation. For this purpose, a search was performed in wetlands atlas and similar studies, and a check was made to see if those wetlands had recordsof flora and fauna studies. Finally, information was sought on protection measures in the area. Consequently, 22 coastal wetlands were registered, and among them, three are internationally recognized as Ramsar sites: Mangroves of Tumbes, Mangrove of San Pedro de Vice, and the Virrilá Estuary, where commendable conservation efforts are underway. However, it was found that more than 77% of the coastal wetlands in northern Peru do not have preservation actions or scientific evidence describing their biodiversity.(AU)

Rainfall effects on Anomalocardia flexuosa densities on the northeastern Brazilian coast using distributed lag models / Efeitos da chuva na densidade de Anomalocardia flexuosa na costa nordeste do Brasil usando modelos de defasagem distribuída

In this study, the effects of precipitation on Anomalocardia flexuosa densities were analyzed from the perspective of temporal delays between the variables. The collections occurred bi-monthly between April 2016 and February 2018 at Mangue Seco beach, Pernambuco, Brazil. Total densities and densities according to the category of size (small, medium and large), and precipitation were analyzed with autocorrelation and cross-correlation functions, with a retrospective analysis of up to 12 months. Distributed lag models were used among the variables. The maximum density was found in June 2017 (378 ind.m2) for the medium-size category of individuals. Significant autocorrelations occurred for densities (total and average size) and precipitation. In cross-correlation functions, high precipitation for a given month was followed by high densities of total and average individuals for up to two months. Distributed lag models also showed significant values between densities (total and medium-sized) and precipitation, which explained more than 50% of the variability of these two groups. The effect of precipitation was responsible for the increase in the density of A. flexuosa for up to two months, mainly by increasing individuals with medium shell lengths.

Neste estudo, foram analisados os efeitos da precipitação nas densidades de Anomalocardia flexuosa sob a perspectiva de atrasos temporais entre as variáveis. As coletas foram bimensais, entre abril de 2016 e fevereiro de 2018, na praia de Mangue Seco, Pernambuco, Brasil. As densidades totais e por categoria de tamanho (pequeno, médio e grande) e a precipitação foram analisadas com as funções de autocorrelação e correlação cruzada, com análise retrospectiva de até 12 meses. Modelos de defasagem distribuída foram empregados entre as variáveis. A densidade máxima foi encontrada em junho de 2017 (378 ind.m²) na categoria de tamanho médio dos indivíduos. Autocorrelações significativas ocorreram nas densidades (total e tamanho médio) e precipitação. Nas funções de correlação cruzada, a alta precipitação de determinado mês foi seguida por alta nas densidades total e de indivíduos médios por até dois meses. Os Modelos de defasagem distribuída também apresentaram valores significativos entre as densidades (total e tamanho médio) e a precipitação, explicando mais de 50% da variabilidade desses dois grupos. O efeito da precipitação foi responsável pelo aumento da densidade de A. flexuosa por até dois meses, principalmente pelo incremento de indivíduos com comprimentos de concha medianos.

Utilização da ozonioterapia como tratamento da endometrite em égua / Use of ozone therapy as endometritis treatment in mare

Conformações perineais inadequadas estão frequentemente associadas à pneumovagina, predispondo animais a processos inflamatórios vaginais e uterinos. Dentre as patologias reprodutivas que acometem éguas, a endometrite é a que ocorre com maior frequência, com forte impacto negativo sobre os índices reprodutivos de um criatório. Para essa afecção, a ozonioterapia se apresenta como tratamento em potencial. Assim, este estudo objetiva relatar um caso de endometrite em égua Quarto de Milha, multípara, de 17 anos de idade, atendida no Hospital Veterinário Sylvio Barbosa Cardoso, da Universidade Estadual de Ceará. Durante a avaliação ginecológica, observou-se alterações na conformação vulvar e teste de Windsucker positivo, indicando pneumovagina. Foram coletadas amostras endometriais para exames laboratoriais, os quais apresentaram citologia com contagem de neutrófilos polimorfonucleares ≥30% e cultura negativa para fungos e bactérias. O exame histopatológico revelou fibrose intersticial endometrial crônica acentuada. Diante do diagnóstico de endometrite, iniciou-se o tratamento com lavagem uterina usando solução ringer lactato ozonizada, seguido por insuflação uterina com ozônio. O animal foi submetido à técnica cirúrgica de Caslick para correção da má conformação vulvar. Após o tratamento, a égua foi inseminada, resultando na coleta de dois embriões viáveis. Diante do exposto, o caso reforça a efetividade da ozonioterapia associada à Caslick para o tratamento de endometrite em éguas.

Dysfunctional perineal conformations are often associated with pneumovagina, predisposing animals to vaginal and uterine inflammatory processes. Among the reproductive pathologies that affect mares, endometritis is the most frequent, with a strong negative impact on the reproductive indices of a farm. For this condition, ozone therapy presents itself as a potential treatment. The study aimed to report a case of endometritis in a 17-year-old multiparous quarter-mile mare, which was attended at the Veterinary Hospital Sylvio Barbosa Cardoso located in the State University of Ceará. During the gynecological evaluation, changes in vulvar conformation and a positive Windsucker test were observed, indicating pneumovagina. Endometrial samples were collected for laboratory exams, resulting in cytology with polymorphonuclear neutrophil count ≥30% and negative culture for fungi and bacteria. The histopathological examination revealed severe chronic endometrial interstitial fibrosis. Regarding the diagnosis of endometritis, the treatment with uterine lavage using ozonated ringer lactate solution was initiated followed by uterine insufflation with ozone. The animal was submitted to Caslick's surgical technique to correct the vulvar malformation. After treatment, the mare was inseminated, resulting in the collection of two viable embryos. In view of the above, the case reinforces the effectiveness of ozone therapy associated with Caslick`s technique for endometritis treatment in mares.