Urethral obstruction secondary to Staphylococcus felis chronic urethritis and hepatoid gland adenoma in a captive ocelot (Leopardus pardalis)

A male adult ocelot (Leopardus pardalis) kept under human care developed anuria, which progressed to death. Grossly, the urinary bladder was markedly dilated and filled with red discolored urine containing blood clots. In addition, the animal had a hepatoid cell adenoma adjacent to the urethra, which likely caused partial urethral occlusion. Microscopically, there was a predominantly neutrophilic, fibrinous and hemorrhagic urethritis, cystitis, and pyelonephritis with intralesional gram-positive cocci. Microbiologic culture followed by MALDI-TOF MS analysis resulted in the identification of isolates from the urine and urethra as Staphylococcus felis.(AU)

Genetic diversity in creole genotypes of Amazon chicory / Diversidade genética em genótipos crioulos de chicória da Amazônia

Amazon chicory is still a little-known vegetable despite its great agronomic potential. The characterization of chicory genotypes concerning genetic divergence is a key step for breeding programs, as it allows the selection of superior individuals and to explore the variability and complementarity of characteristics via interbreeding between newly generated genotypes. In this context, this study aimed to evaluate the genetic divergence among Amazon chicory creole genotypes from the northern Brazilian states of Pará and Rondônia based on morpho-agronomic traits. We conducted an experiment in a randomized block design with eight chicory genotypes (treatments) and four replications. Both quantitative and qualitative characteristics were evaluated. Genetic divergence was estimated via squared generalized Mahalanobis distance (D2 ), considering only quantitative characters, and the genotypes were subsequently clustered via the UPGMA method. Analysis of variance showed significant differences among genotypes for all studied characteristics, except shoot fresh weight. The UPGMA grouped the genotypes into three clusters, which demonstrated that the genotypes from Colares and Santarém Novo (Pará) (Chic-02 and Chic-04) were the most divergent as compared to the genotypes from Castanhal and Santa Isabel do Pará (Pará). Qualitative characteristics showed a monomorphic behavior and, therefore, were not used to assess genetic divergences. To obtain segregating populations with complementary characteristics, crossbreeding between the two most divergent clusters is recommended.(AU)

A chicória da Amazônia é uma hortaliça ainda pouco conhecida, mas com grande potencial agronômico. A caracterização dos genótipos de chicória quanto a divergência genética é um importante passo para programas de melhoramento genético, pois permite selecionar indivíduos superiores e explorar a variabilidade e a complementariedade de características, a partir dos novos genótipos gerados. Nesse contexto, o objetivo deste estudo foi avaliar a divergência genética de genótipos crioulos de chicória da Amazônia dos estados do Pará e Rondônia, com base em caracteres morfoagronômicos. Realizou-se um experimento em delineamento de blocos ao acaso, com oito genótipos de chicória (tratamentos) e quatro repetições. Foram avaliadas características quantitativas e qualitativas. A divergência genética foi estimada a partir da distância quadrada generalizada de Mahalanobis (D2 ), levando em consideração apenas os caracteres quantitativos, e os genótipos foram agrupados pelo método UPGMA. A análise de variância evidenciou diferença significativa entre os genótipos para todas as características, exceto massa fresca. O UPGMA agrupou os genótipos em três grupos, sendo os genótipos de Colares e Santarém Novo (Pará) (Chic-02 e Chic-04) os mais divergentes em comparação com os genótipos da região de Castanhal e Santa Isabel do Pará (Pará). As características qualitativas apresentaram padrão monomórfico, não sendo, portanto, utilizadas para avaliar a divergência. Para obter populações segregantes com complementariedade de características, recomenda-se o cruzamento entre os dois grupos mais divergentes.(AU)

A citogenética da sexagem molecular de aves selvagens monomórficas e a influência negativa de anomalias pigmentares: revisão de literatura / The cytogenetics of the molecular sexage of wild birds monomorphics and the negative influence of anomalies pigmentary: literature review

O Brasil apresenta a segunda maior biodiversidade de aves do mundo, entretanto, lidera o ranking de aves ameaçadas de extinção, sobretudo, devido às ações antropológicas sobre as aves e seu habitat. Logo, a sexagem citogenética, através da técnica de PCR e estudo de cariótipos, possibilita a identificação e manutenção de espécies ameaçadas. Outrossim, existem anomalias pigmentares que modificam os fenótipos das aves e, consequentemente, prejudicam a identificação de espécies. Assim, esse trabalho objetivou, através de uma revisão de literatura, discorrer sobre a importância da citogenética na sexagem molecular de aves selvagens monomórficas e evidenciar a influência negativa de anomalias pigmentares.

Brazil has the second largest bird biodiversity in the world, however, it leads the ranking of endangered birds, mainly due to anthropological actions on birds and their habitat. Therefore, cytogenetic sexing, through the PCR technique and the study of karyotypes, enables the identification and maintenance of endangered species. Furthermore, there are pigmentary anomalies that modify the phenotypes of birds and, consequently, hinder the identification of species. Thus, this study aimed, through a literature review, to discuss the importance of cytogenetics in the molecular sexing of monomorphic wild birds and to highlight the negative influence of pigmentary anomalies.

Cutaneous mast cell tumor in a captive Bush dog (Speothos venaticus): pathological and immunophenotypical aspects - case report / Mastocitoma cutâneo em um cachorro-do-mato vinagre (Speothos venaticus) de cativeiro: aspectos patológicos e imunofenotípicos - relato de caso

A senile male captive bush dog (Speothos venaticus) presented a small perianal cutaneous nodule. Histologically, there was an ulcerated round cell tumor composed of well differentiated mast cells with abundant intracytoplasmic purple Giemsa-positive granules, with a diffuse eosinophilic infiltrate. Immunohistochemistry revealed that 30% of the neoplastic cells were positive for Kit in the cytoplasm and cell membrane, and all neoplastic cells were negative for MAC and CD3. Less than 10% of the neoplastic cells were positive for Ki67. At necropsy other primary tumors were identified in this animal, including an intestinal adenoma, an adrenal cortex adenoma and a testicular interstitial cell tumor.(AU)

Um cachorro-vinagre (Speothos venaticus) apresentou um nódulo cutâneo pequeno na região perianal. Histologicamente havia neoplasia cutânea de células redondas e ulcerada, constituída por mastócitos bem diferenciados, com abundantes grânulos citoplasmáticos metacromáticos na coloração de Giemsa e infiltrado eosinofílico difuso. A imuno-histoquímica demonstrou que 30% das células neoplásicas eram positivas para a proteína Kit no citoplasma e na membrana celular. As células foram negativas para MAC e CD3. Menos de 10% das células neoplásicas foram positivas para Ki67. Durante a necropsia, foram identificados outros tumores primários, como adenoma intestinal, adenoma cortical da adrenal e tumor de células intersticiais do testículo.(AU)

Tubular adenoma pyloric-gland type in a cow

The paper describes a typical Pyloric-Gland Type Tubular Adenoma founded in a 7 years old holstein cow. At necropsy, macroscopic examination showed a pedunculated polypoid formation measuring 4.0 cm in its largest diameter in the mucous membrane of the gallbladders body region, with a delicate base in the form of a rod measuring 2.5 cm in length. Histologically analysis showed a tubular cell proliferation covered with cylindrical epithelium, which secreted mucus, with regular, vesicular nuclei with small and regular nucleoli. The stroma was represented by a delicate and loosened, vascularized connective tissue and edema. In the veterinary literature, there is no mention of the occurrence of Pyloric-Gland Type Tubular Adenoma in cattle as well a published description about it.(AU)

Tubular adenoma pyloric-gland type in a cow

The paper describes a typical Pyloric-Gland Type Tubular Adenoma founded in a 7 years old holstein cow. At necropsy, macroscopic examination showed a pedunculated polypoid formation measuring 4.0 cm in its largest diameter in the mucous membrane of the gallbladder’s body region, with a delicate base in the form of a rod measuring 2.5 cm in length. Histologically analysis showed a tubular cell proliferation covered with cylindrical epithelium, which secreted mucus, with regular, vesicular nuclei with small and regular nucleoli. The stroma was represented by a delicate and loosened, vascularized connective tissue and edema. In the veterinary literature, there is no mention of the occurrence of Pyloric-Gland Type Tubular Adenoma in cattle as well a published description about it.

Histopathological study of canine sebaceous tumors and their association with PCNA expression by immunohistochemistry / Estudo histopatológico de tumores sebáceos caninos e sua associação com a expressão de PCNA por imuno-histoquímica

Sebaceous tumors are common in dogs. These tumors include both benign and malignant lesions. Immunohistochemical evaluation of these tumors can aggregate information regarding the origin and degree of malignancy of the lesions. Focusing on this matter, sixty-one samples including normal skin and sebaceous tumors were selected from dogs of various breeds and ages, with no predilection for sex, from the archive of Veterinary Pathology Service of Federal Fluminense University, Niterói/RJ, Brazil. The samples underwent to histological processing, routine staining and immunohistochemistry with anti-PCNA (proliferating cell nuclear antigen). Descriptive statistical analysis was performed, the Wilcoxon-Mann-Whitney test was used to compare the distribution of anti-PCNA labelling in different groups of variables. In case there were more than two groups, the Analysis of Variance (ANOVA) test was performed. The mean age of the affected animals was 10.56 years. The most affected breeds were Caniches and Cocker Spaniels, as well as mixed breed animals. There was immunostaining of PCNA in both benign and malignant tumors, as well as in hyperplasic lesions with varying intensity. Most of the tumors were neoplasms which represented 67.27% of the total sample; within these, 75.00% were benign. The most frequent neoplasm was sebaceous adenoma (37.74%). Results indicated no statistical difference in the distribution of anti-PCNA labelling between the groups of sex, age, reproductive status, localization, size of tumor, and histopathological diagnosis. Although there are not many studies analyzing anti-PCNA labelling in sebaceous tumors, several of them pointed out to the predictive value in other neoplasms. With this matter in mind, we intended to evaluate the expression of anti-PCNA in canine sebaceous tumor and a possible association with the malignancy of the lesions.

Tumores sebáceos são comuns em cães. Tais tumores incluem lesões benignas e malignas. A avaliação imunohistoquímica desses tumores pode agregar informações sobre a origem e o grau de malignidade das lesões. Para este fim, sessenta e uma amostras, incluindo pele normal e tumores sebáceos foram selecionadas de cães de várias raças e idades, sem predileção por sexo, do arquivo do Serviço de Patologia Veterinária da Universidade Federal Fluminense, Niterói/RJ, Brasil. As amostras passaram por processamento histológico, coloração de rotina e imuno-histoquímica com anti-PCNA (proliferating cell nuclear antigen). Foram realizadas análises estatísticas descritivas além dos testes de Wilcoxon-Mann-Whitney para comparar a distribuição da marcação de anti-PCNA entre grupos de variáveis. Para variáveis com mais de dois grupos, aplicou-se a Análise de Variância (ANOVA). A idade média dos animais afetados foi de 10.56 anos. As raças mais afetadas foram Caniches e Cocker Spaniel, e ainda animais sem raça definida. Houve imunomarcação de PCNA em tumores benignos, malignos, e ainda em lesões hiperplásicas com intensidade variada. A maioria dos tumores eram neoplásicos representando 67.92% do total; destes, 75.00% eram benignos. O adenoma sebáceo foi a neoplasia mais frequente (37.74%). Não foram encontradas diferenças significativas nas distribuições de anti-PCNA entre os grupos das variáveis sexo, idade, status reprodutivo, localização e tamanho do tumor e diagnóstico histopatológico. Embora não haja estudos com anti-PCNA em tumores sebáceos caninos, numerosas publicações apontam seu valor preditivo em outras neoplasias. Com isso, a finalidade deste estudo foi avaliar a expressão de anti-PCNA em tumores sebáceos caninos e sua possível associação com a malignidade das lesões.

Transferability of heterologous microsatellite primers in Leiarius marmoratus / Transferibilidade de primers microssatélites heterólogos em Leiarius marmoratus

The Amazonian Jundiá (Leiarius marmoratus) (Siluliformes: Pimelodidae) is a species of catfish with social and economic importance in some South American countries such as Brazil and Colombia. Genetic evaluation of this species is limited due to the lack of specific molecular markers, hindering studies on genetic diversity and structure in animals under captive conditions or in natural populations. The objective of the present study was to evaluate the transferability of heterologous microsatellite markers in Leiarius marmoratus. Thirty-two heterologous primers were tested in L. marmoratus. The primers that presented the best standards were applied to 20 specimens, and the number of alleles (Na), number of effective alleles (Ne), gene diversity per Locus (GdL) and percentage of amplification failure (Md) were calculated. Eleven primers demonstrated satisfactory transferability patterns, all from the fish of the Pimelodidae family, of which, seven were monomorphic and four polymorphic. The eleven markers presented low percentage of Md (mean was 5.9% samples per locus). Na varied from one to two alleles per locus, revealing low polymorphism in the evaluated samples. The mean Ne and GdL numbers were 1.77 and 0.32, respectively. The transferability of the heterologous microsatellite loci in L. marmoratus was shown to be possible. However, further tests are needed to apply these markers inpopulation genetic studies.

O Jundiá da Amazônia (Leiarius marmoratus) (Siluliformes: Pimelodidae) é uma espécie de catfish com importância social e econômica em alguns países Sul americanos como Brasil e Colômbia. A avaliação genética dessa espécie é limitada devido a ausência de marcadores moleculares específicos para L. marmoratus, dificultando estudos sobre a diversidade e estrutura genética em animais em condições de cativeiro ou em populações naturais. O objetivo do presente estudo foi avaliar a transferibilidade de 32 marcadores microssatélites heterólogos em Leiarius marmoratus. Os primers que apresentaram melhores padrões foram aplicados em 20 espécimes, sendo calculados o número de alelos (Na), número de alelos efetivos (Ne), diversidade gênica por Locus (GdL) e porcentagem de falha na amplificação (Md). Onze primers demonstraram padrões satisfatórios de transferibilidade, todos oriundos de peixes da família Pimelodidae, dos quais sete foram monomórficos e quatro polimórficos. Os onze marcadores apresentaram baixa porcentagem de Md (média 5,9% amostras por loco). O Na variou deum a dois alelos por loco, revelando baixo polimorfismo nas amostras avaliadas. O número médio de Ne e GdL foram 1,77 e 0,32, respectivamente. Estudos genéticos envolvendo indivíduos de diferentes regiões poderão apresentar novos alelos e maior número de loci polimórficos. A transferibilidade de loci microssatélites heterólogos em L. marmoratus se mostrou possível, no entanto, novos testes são necessários para aplicação desses marcadores em estudos genéticos populacionais.

Tricoblastoma em cão: uma análise clínica, diagnóstica e terapêutica / Trichoblastoma in a dog: a clinical, diagnostic and therapeutic analysis

Background: Trichoblastoma is a rare skin neoplasm derived from the primitive hair follicle, having epithelial and mesenchymal components. Despite being commonly described as a benign tumor, there are reports in the literature of tumors with characteristics of malignant neoplasia. It affects adult dogs and cats, with no predilection for sex. Although rare, in studies in Brazil the pathology shows variable rates of occurrence. This work describes a case of tricoblastoma in a 4-year-old mixed breed dog, submitted to care at the Veterinary Hospital of the Federal University of Western Bahia (HVU-UFOB). Case: A 4-year-old male mixed breed dog with 7 kg of body weight, was referred to the HVU-UFOB. The main complaint reported by the canine’s tutor was an increase in volume in the region of the animal’s face, which evolved slowly, associated with itching, bleeding and sensitivity to touch. During the physical examination, a round, elevated mass was observed in the subcutaneous tissue of the left face, in the mandible region, with local alopecia. On palpation, the neoformation showed a firm consistency, well-defined and fluctuating, with no solutions for continuity or discomfort to touch. The animal’s physiological parameters were within the normal for the species. CBC, chest X-rays, abdominal ultrasound and aspiration cytology of the mass were requested. The blood count and image tests did not reveal any changes. On cytological examination, small, grouped basal epithelial cells were observed, characterized by a high nucleus-cytoplasm ratio, monomorphic nuclei and intensely basophilic and pigmented cytoplasm, a suggestive diagnosis of basal cell tumor. Due to the findings, an excisional biopsy of the mass was performed, with margins of 2 cm. The mass with its margins was sent for histopathological examination at the Animal Pathology Laboratory of the HVU...

Tricoblastoma em cão: uma análise clínica, diagnóstica e terapêutica / Trichoblastoma in a dog: a clinical, diagnostic and therapeutic analysis

Background: Trichoblastoma is a rare skin neoplasm derived from the primitive hair follicle, having epithelial and mesenchymal components. Despite being commonly described as a benign tumor, there are reports in the literature of tumors with characteristics of malignant neoplasia. It affects adult dogs and cats, with no predilection for sex. Although rare, in studies in Brazil the pathology shows variable rates of occurrence. This work describes a case of tricoblastoma in a 4-year-old mixed breed dog, submitted to care at the Veterinary Hospital of the Federal University of Western Bahia (HVU-UFOB). Case: A 4-year-old male mixed breed dog with 7 kg of body weight, was referred to the HVU-UFOB. The main complaint reported by the canines tutor was an increase in volume in the region of the animals face, which evolved slowly, associated with itching, bleeding and sensitivity to touch. During the physical examination, a round, elevated mass was observed in the subcutaneous tissue of the left face, in the mandible region, with local alopecia. On palpation, the neoformation showed a firm consistency, well-defined and fluctuating, with no solutions for continuity or discomfort to touch. The animals physiological parameters were within the normal for the species. CBC, chest X-rays, abdominal ultrasound and aspiration cytology of the mass were requested. The blood count and image tests did not reveal any changes. On cytological examination, small, grouped basal epithelial cells were observed, characterized by a high nucleus-cytoplasm ratio, monomorphic nuclei and intensely basophilic and pigmented cytoplasm, a suggestive diagnosis of basal cell tumor. Due to the findings, an excisional biopsy of the mass was performed, with margins of 2 cm. The mass with its margins was sent for histopathological examination at the Animal Pathology Laboratory of the HVU...(AU)

Transferability of heterologous microsatellite primers in Leiarius marmoratus / Transferibilidade de primers microssatélites heterólogos em Leiarius marmoratus

The Amazonian Jundiá (Leiarius marmoratus) (Siluliformes: Pimelodidae) is a species of catfish with social and economic importance in some South American countries such as Brazil and Colombia. Genetic evaluation of this species is limited due to the lack of specific molecular markers, hindering studies on genetic diversity and structure in animals under captive conditions or in natural populations. The objective of the present study was to evaluate the transferability of heterologous microsatellite markers in Leiarius marmoratus. Thirty-two heterologous primers were tested in L. marmoratus. The primers that presented the best standards were applied to 20 specimens, and the number of alleles (Na), number of effective alleles (Ne), gene diversity per Locus (GdL) and percentage of amplification failure (Md) were calculated. Eleven primers demonstrated satisfactory transferability patterns, all from the fish of the Pimelodidae family, of which, seven were monomorphic and four polymorphic. The eleven markers presented low percentage of Md (mean was 5.9% samples per locus). Na varied from one to two alleles per locus, revealing low polymorphism in the evaluated samples. The mean Ne and GdL numbers were 1.77 and 0.32, respectively. The transferability of the heterologous microsatellite loci in L. marmoratus was shown to be possible. However, further tests are needed to apply these markers inpopulation genetic studies.(AU)

O Jundiá da Amazônia (Leiarius marmoratus) (Siluliformes: Pimelodidae) é uma espécie de catfish com importância social e econômica em alguns países Sul americanos como Brasil e Colômbia. A avaliação genética dessa espécie é limitada devido a ausência de marcadores moleculares específicos para L. marmoratus, dificultando estudos sobre a diversidade e estrutura genética em animais em condições de cativeiro ou em populações naturais. O objetivo do presente estudo foi avaliar a transferibilidade de 32 marcadores microssatélites heterólogos em Leiarius marmoratus. Os primers que apresentaram melhores padrões foram aplicados em 20 espécimes, sendo calculados o número de alelos (Na), número de alelos efetivos (Ne), diversidade gênica por Locus (GdL) e porcentagem de falha na amplificação (Md). Onze primers demonstraram padrões satisfatórios de transferibilidade, todos oriundos de peixes da família Pimelodidae, dos quais sete foram monomórficos e quatro polimórficos. Os onze marcadores apresentaram baixa porcentagem de Md (média 5,9% amostras por loco). O Na variou deum a dois alelos por loco, revelando baixo polimorfismo nas amostras avaliadas. O número médio de Ne e GdL foram 1,77 e 0,32, respectivamente. Estudos genéticos envolvendo indivíduos de diferentes regiões poderão apresentar novos alelos e maior número de loci polimórficos. A transferibilidade de loci microssatélites heterólogos em L. marmoratus se mostrou possível, no entanto, novos testes são necessários para aplicação desses marcadores em estudos genéticos populacionais.(AU)

Natural infection by Trypanosoma cruzi in a dog from Bahia state - northeast of Brazil

Background: Chagas Disease (CD) is a parasitic anthropozoonosis caused by the Trypanosoma cruzi (T. cruzi), a protozoan transmitted by insects from the Reduviidae family. Several species of wild and domestic animals, humans included, are susceptible, developing acute clinical signals (myocarditis and cardiac arrhythmias) or chronic signals (drop on the performance and syncope) of the disease. In Brazil, the disease in dogs shows variable indexes of occurrence. The present paper describes the natural infection by T. cruzi in a canine living in the city of Barra, in the state of Bahia, Brazil, a region classified as high risk of vulnerability for the CD in the northeast of Brazil. Case: A 9-year-old male dog, non-specific breed, was referred to the clinic the Veterinary Clinic for Small Animals, at the Veterinary Medical Teaching Hospital), at the Federal University of the West of Bahia. Its owner reported the presence of bloody secretion in the penile region. During the physical exam it was possible to observe an increase in the volume of the penis base. All of the animal’s physiological parameters were within normal levels. Blood samples and cytological laminas from the lesioned area were collected and sent to the Laboratory of Veterinary Clinical Pathology in the same institution. The cytological exam showed monomorphic population of great round cells with a round nucleus, condensed chromatin and one to two prominent nucleolus, abundant and slightly basophilic cytoplasm, with multiple stippled vacuoles, thus confirming a Transmissible venereal tumor (TVT) case. With the blood exam one observed a thrombocytopenia, neutrophilia with a deviation on the left and the presence of a trypomastigote form of Trypanosoma sp. in the blood smears...

Natural infection by Trypanosoma cruzi in a dog from Bahia state - northeast of Brazil

Background: Chagas Disease (CD) is a parasitic anthropozoonosis caused by the Trypanosoma cruzi (T. cruzi), a protozoan transmitted by insects from the Reduviidae family. Several species of wild and domestic animals, humans included, are susceptible, developing acute clinical signals (myocarditis and cardiac arrhythmias) or chronic signals (drop on the performance and syncope) of the disease. In Brazil, the disease in dogs shows variable indexes of occurrence. The present paper describes the natural infection by T. cruzi in a canine living in the city of Barra, in the state of Bahia, Brazil, a region classified as high risk of vulnerability for the CD in the northeast of Brazil. Case: A 9-year-old male dog, non-specific breed, was referred to the clinic the Veterinary Clinic for Small Animals, at the Veterinary Medical Teaching Hospital), at the Federal University of the West of Bahia. Its owner reported the presence of bloody secretion in the penile region. During the physical exam it was possible to observe an increase in the volume of the penis base. All of the animals physiological parameters were within normal levels. Blood samples and cytological laminas from the lesioned area were collected and sent to the Laboratory of Veterinary Clinical Pathology in the same institution. The cytological exam showed monomorphic population of great round cells with a round nucleus, condensed chromatin and one to two prominent nucleolus, abundant and slightly basophilic cytoplasm, with multiple stippled vacuoles, thus confirming a Transmissible venereal tumor (TVT) case. With the blood exam one observed a thrombocytopenia, neutrophilia with a deviation on the left and the presence of a trypomastigote form of Trypanosoma sp. in the blood smears...(AU)

Reticulose pagetoide em um cão: relato de caso / Pagetoid reticulosis in a dog: case report

Reticulose pagetoide é a expressão utilizada para uma das mais raras formas de linfoma descritas em cães. Trata-se de um distúrbio linfoproliferativo cutâneo que emerge de linfócitos T intraepidérmicos. Descreve-se aqui um caso de reticulose pagetoide com apresentação localizada, muito semelhante à doença de Woringer-Kolopp dos humanos, que acometeu um cão, Boxer, fêmea de 10 anos de idade. O paciente foi atendido devido ao aparecimento de um nódulo na região nasolabial, com evolução clínica de cerca de 30 dias. Histologicamente o nódulo era composto por uma proliferação linfoide intraepidérmica e anexal. Os linfócitos neoplásicos, células T confirmadas por meio da imunomarcação positiva para CD3 e negativa para CD79, eram grandes células redondas e monomórficas. Apesar de rara, a reticulose pagetoide é um distúrbio linfoproliferativo de fácil suspeita com base apenas na morfologia celular. Esse neoplasma deverá ser lembrado sempre que um padrão linfoide intraepidérmico estiver presente.(AU)

Reticulosis pagetoide is an expression used for one of the rare forms of lymphoma described in dogs. It is a cutaneous lymphoproliferative disorder that emerges from intraepidermal T-cells. We describe a case of pagetoid reticulosis with localized presentation, very similar to Woringer-Kolopp's disease in humans, which affected a 10-year-old Boxer female. The patient was seen due to the appearance of a nodule in the nasolabial region with clinical evolution of about 30 days. Histologically, it was composed of an intraepidermal and annexal lymphoid proliferation. Neoplastic lymphocytes, T-cells confirmed by CD3-positive and CD79-negative immunolabeling, were large, round, and monomorphic cells. Although rare, pagetoid reticulosis is an easily suspected lymphoproliferative disorder based only on cell morphology. This neoplasm should be remembered whenever an intraepidermal lymphoid pattern is present.(AU)

Tumor venéreo transmissível cutâneo sem envolvimento genital em cão macho / Transmissible cutaneous venereal tumor without genital involvement in a male dog / Cutánea tumor venéreo transmisible sin la participación genital en perro macho

O tumor venéreo transmissível (TVT) trata-se de uma neoplasia de células redondas, transmitida a partir do transplante de células neoplásicas, altamente contagiosa, que afeta principalmente a genitália externa de caninos e também regiões extragenitais. A forma cutânea geralmente acomete a região de face e membros, secundária a um foco genital primário de forma simultânea. Relata-se um caso de TVT cutâneo sem envolvimento genital em um cão macho sem padrão de raça definida (SPRD), quatro anos de idade, semidomiciliado, com queixa principal de aparecimento de lesão única, de caráter nodular, friável, ulcerada com exsudação sanguinolenta na região cervical lateral. Foi realizada punção aspirativa com agulha fina para avaliação citológica, que demostrou predomínio de células redondas monomórficas, com vacuolização citoplasmática, citoplasma basofílico e escasso, além de núcleos ovais e centralizados, citologia esta compatível com TVT. Adotou-se o protocolo de tratamento quimioterápico com sulfato de vincristina 0,025 mg/kg a cada sete dias, com regressão completa do tumor após seis semanas. O envolvimento do tecido cutâneo sem acometimento venéreo é raro, e diante disso o TVT pode ser incluído como diagnóstico diferencial de lesões nodulares ou tumorais isoladas.(AU)

The transmissible venereal tumor (TVT) is a round cells tumor, transmitted from the highly contagious neoplastic cell transplantation, which mainly affects the external genitalia of dogs and also extragenital regions. The cutaneous form usually affects the region of the face and limbs, secondary to a primary genital focus simultaneously. A case of cutaneous TVT without genital involvement is reported in a four-year-old male, without racial standard set, half-domiciled, with a major complaint of a nodular, friable and ulcerated lesion with bloody exudation in the lateral cervical region. Fine needle aspiration was performed for cytological evaluation, and presented a monomorphic round cell population, with cytoplasmic vacuolization, basophilic and scarce cytoplasm, oval and centralized nuclei. The cytology evaluation was compatible with TVT. The chemotherapy protocol was adopted with vincristine sulphate at 0.025 mg/kg every 7 days. Complete regression of the tumor mass was observed after six weeks. Involvement of the cutaneous tissue without venereal involvement is uncommon, and there after the TVT is included as a differential diagnosis of isolated nodular cutaneous lesions.(AU)

El tumor venéreo transmisible (TVT) se trata de una neoplasia de células redondas, transmitida a partir del transplante de células neoplásicas, altamente contagiosa, que afecta principalmente a los genitales externos de los caninos y también a las regiones extragenitales. La forma cutánea generalmente acomete la región de cara y miembros, secundaria a un foco genital primario de forma simultánea. Se relata un caso de TVT cutáneo sin implicación genital en un perro macho, cuatro años de edad, semidomiciliado, con queja principal de aparición de lesión única, de carácter nodular, friable, ulcerada con exudación sanguinolenta en la región cervical lateral. Se realizó una punción aspirativa con aguja fina para evaluación citológica, y obtuvo como resultado el predominio de células redondas monomórficas, con vacuolización citoplasmática, citoplasma basofílico y escaso, además de núcleos ovalados y centralizados, citología esta compatible con TVT. Se adoptó el protocolo de tratamiento quimioterápico con sulfato de vincristina 0,025 mg / kg cada siete días, regresión completa del tumor después de seis semanas. La participación del tejido cutáneo sin afectación venérea es rara, y ante todo el TVT se incluye como diagnóstico diferencial de lesiones nodulares o tumorales aisladas.(AU)

Reticulose pagetoide em um cão: relato de caso / Pagetoid reticulosis in a dog: case report

Reticulose pagetoide é a expressão utilizada para uma das mais raras formas de linfoma descritas em cães. Trata-se de um distúrbio linfoproliferativo cutâneo que emerge de linfócitos T intraepidérmicos. Descreve-se aqui um caso de reticulose pagetoide com apresentação localizada, muito semelhante à doença de Woringer-Kolopp dos humanos, que acometeu um cão, Boxer, fêmea de 10 anos de idade. O paciente foi atendido devido ao aparecimento de um nódulo na região nasolabial, com evolução clínica de cerca de 30 dias. Histologicamente o nódulo era composto por uma proliferação linfoide intraepidérmica e anexal. Os linfócitos neoplásicos, células T confirmadas por meio da imunomarcação positiva para CD3 e negativa para CD79, eram grandes células redondas e monomórficas. Apesar de rara, a reticulose pagetoide é um distúrbio linfoproliferativo de fácil suspeita com base apenas na morfologia celular. Esse neoplasma deverá ser lembrado sempre que um padrão linfoide intraepidérmico estiver presente.(AU)

Reticulosis pagetoide is an expression used for one of the rare forms of lymphoma described in dogs. It is a cutaneous lymphoproliferative disorder that emerges from intraepidermal T-cells. We describe a case of pagetoid reticulosis with localized presentation, very similar to Woringer-Kolopp's disease in humans, which affected a 10-year-old Boxer female. The patient was seen due to the appearance of a nodule in the nasolabial region with clinical evolution of about 30 days. Histologically, it was composed of an intraepidermal and annexal lymphoid proliferation. Neoplastic lymphocytes, T-cells confirmed by CD3-positive and CD79-negative immunolabeling, were large, round, and monomorphic cells. Although rare, pagetoid reticulosis is an easily suspected lymphoproliferative disorder based only on cell morphology. This neoplasm should be remembered whenever an intraepidermal lymphoid pattern is present.(AU)

Tumor venéreo transmissível cutâneo sem envolvimento genital em cão macho / Transmissible cutaneous venereal tumor without genital involvement in a male dog / Cutánea tumor venéreo transmisible sin la participación genital en perro macho

O tumor venéreo transmissível (TVT) trata-se de uma neoplasia de células redondas, transmitida a partir do transplante de células neoplásicas, altamente contagiosa, que afeta principalmente a genitália externa de caninos e também regiões extragenitais. A forma cutânea geralmente acomete a região de face e membros, secundária a um foco genital primário de forma simultânea. Relata-se um caso de TVT cutâneo sem envolvimento genital em um cão macho sem padrão de raça definida (SPRD), quatro anos de idade, semidomiciliado, com queixa principal de aparecimento de lesão única, de caráter nodular, friável, ulcerada com exsudação sanguinolenta na região cervical lateral. Foi realizada punção aspirativa com agulha fina para avaliação citológica, que demostrou predomínio de células redondas monomórficas, com vacuolização citoplasmática, citoplasma basofílico e escasso, além de núcleos ovais e centralizados, citologia esta compatível com TVT. Adotou-se o protocolo de tratamento quimioterápico com sulfato de vincristina 0,025 mg/kg a cada sete dias, com regressão completa do tumor após seis semanas. O envolvimento do tecido cutâneo sem acometimento venéreo é raro, e diante disso o TVT pode ser incluído como diagnóstico diferencial de lesões nodulares ou tumorais isoladas.

The transmissible venereal tumor (TVT) is a round cells tumor, transmitted from the highly contagious neoplastic cell transplantation, which mainly affects the external genitalia of dogs and also extragenital regions. The cutaneous form usually affects the region of the face and limbs, secondary to a primary genital focus simultaneously. A case of cutaneous TVT without genital involvement is reported in a four-year-old male, without racial standard set, half-domiciled, with a major complaint of a nodular, friable and ulcerated lesion with bloody exudation in the lateral cervical region. Fine needle aspiration was performed for cytological evaluation, and presented a monomorphic round cell population, with cytoplasmic vacuolization, basophilic and scarce cytoplasm, oval and centralized nuclei. The cytology evaluation was compatible with TVT. The chemotherapy protocol was adopted with vincristine sulphate at 0.025 mg/kg every 7 days. Complete regression of the tumor mass was observed after six weeks. Involvement of the cutaneous tissue without venereal involvement is uncommon, and there after the TVT is included as a differential diagnosis of isolated nodular cutaneous lesions.

El tumor venéreo transmisible (TVT) se trata de una neoplasia de células redondas, transmitida a partir del transplante de células neoplásicas, altamente contagiosa, que afecta principalmente a los genitales externos de los caninos y también a las regiones extragenitales. La forma cutánea generalmente acomete la región de cara y miembros, secundaria a un foco genital primario de forma simultánea. Se relata un caso de TVT cutáneo sin implicación genital en un perro macho, cuatro años de edad, semidomiciliado, con queja principal de aparición de lesión única, de carácter nodular, friable, ulcerada con exudación sanguinolenta en la región cervical lateral. Se realizó una punción aspirativa con aguja fina para evaluación citológica, y obtuvo como resultado el predominio de células redondas monomórficas, con vacuolización citoplasmática, citoplasma basofílico y escaso, además de núcleos ovalados y centralizados, citología esta compatible con TVT. Se adoptó el protocolo de tratamiento quimioterápico con sulfato de vincristina 0,025 mg / kg cada siete días, regresión completa del tumor después de seis semanas. La participación del tejido cutáneo sin afectación venérea es rara, y ante todo el TVT se incluye como diagnóstico diferencial de lesiones nodulares o tumorales aisladas.

Papillary renal adenoma in a toco toucan (Ramphastos toco)

Primary renal neoplasms are infrequent in domestic animals and birds, being budgerigars an exception. This report describes a papillary renal adenoma in a toco toucan (Ramphastos toco), which was euthanized due to poor prognosis after surgical intervention of phalanx fractures in both wings. Grossly, the caudal pole of the right kidney had a nodular lesion measuring 3.5 x 1.7 x 1.5 cm. Microscopically, there was a well delimited neoplasm arranged in large and coalescent papillary structures composed by a single layer of epithelial cells. There was mild to moderate nuclear and cytoplasmic pleomorphism, whereas mitotic figures were absent. In conclusion, this tumor was considered as an accidental finding, which is similar to the majority of adenomas reported in domestic mammals.

Papillary renal adenoma in a toco toucan (Ramphastos toco)

Primary renal neoplasms are infrequent in domestic animals and birds, being budgerigars an exception. This report describes a papillary renal adenoma in a toco toucan (Ramphastos toco), which was euthanized due to poor prognosis after surgical intervention of phalanx fractures in both wings. Grossly, the caudal pole of the right kidney had a nodular lesion measuring 3.5 x 1.7 x 1.5 cm. Microscopically, there was a well delimited neoplasm arranged in large and coalescent papillary structures composed by a single layer of epithelial cells. There was mild to moderate nuclear and cytoplasmic pleomorphism, whereas mitotic figures were absent. In conclusion, this tumor was considered as an accidental finding, which is similar to the majority of adenomas reported in domestic mammals.(AU)

Bartholins gland adenoma in a Saanen goat / Adenoma de glândula de Bartholin em cabra da raça Saanen

Tumors affecting Bartholins gland are considered rare in human medicine; there are few reports in the veterinary literature, with descriptions occurring only in cows. This article described the clinical and pathological findings associated with Bartholins gland adenoma in a goat. Clinically, a 7-year-old pregnant Saanen goat presented bilateral enlargement of the vulva that did not regress spontaneously after parturition. Grossly, these vulvar masses were multilobulated, contained cystic areas from which oozed a whitish fluid. Histopathology revealed an adenoma characterized by the proliferation of irregularly shaped neoplastic epithelial cells that formed tubular to glandular-like structures. These neoplastic cells demonstrated moderate anisokaryosis and evident nucleoli. The intratumoral proliferation index (PI) was estimated by immunoreactivity with the protein ki-67. Further, the glandular-like structures produced a Periodic Acid-Schiff positive secretion. A diagnosis of Bartholins gland adenoma was established due to the anatomic location of the neoplastic growths, the histopathological features, and the PI of the tumor.(AU)

Tumores que afetam a glândula de Bartholin são considerados raros em humanos e há poucos relatos na medicina veterinária, sendo descrito somente em vacas. Este artigo descreve os achados clínicos e patológicos associados ao adenoma na glândula de Bartholin em uma cabra. O animal da raça Saanen, com sete anos de idade e gestante apresentava aumento de volume bilateral na região da vulva, que não regrediu após o parto. Macroscopicamente, a massa era multilobulada, com presença de vários cistos preenchidos por uma secreção esbranquiçada. A histopatologia revelou um adenoma, caracterizado por proliferação de células epiteliais de formato irregular que formavam estruturas tubulares semelhantes à glândulas. Estas células neoplásicas apresentavam anisocariose moderada e nucléolos evidentes. O índice de proliferação celular intratumoral (PI) foi estimado por imunorreatividade à proteína ki-67. Além disso, a secreção produzida por essas estruturas do tipo glandular, foi positiva na coloração do Ácido Periódico-Schiff. O diagnóstico de adenoma da glândula de Bartholin foi estabelecido pela localização anatômica dos tumores neoplásicos, características histopatológicas e à PI do tumor.(AU)