Review: biological, antioxidant and phytochemical activities of Musa spp / Revisão: atividades biológicas, antioxidante e fitoquímica de Musa spp

Banana (Musa spp.) is a food with high nutritional value. Studies about its compounds have increased considerably due to the antioxidant and biological activities of the fruit. Thus, this article synthesized and organized data related to the phytochemical constituents, as well as antioxidant and biological activities of Musa acuminata, Musa balbisiana and Musa paradisiaca, and evaluated the mutual influence and correlation of these activities. A bibliographic review was performed using the scientific databases Google Scholar, SciELO, Periódicos CAPES, Scientific Electronic Library Online (SciELO), Science Direct, PubMed and Scopus, applying the following terms: Musa spp., antioxidant, biological and phytochemical activities, combined by the Boolean operator AND. A total of 28 articles were selected from 2017 to 2022. The results indicated that bananas are rich in flavonoids, showed good performance in DPPH and FRAP antioxidant assays and have antibacterial, anticancer and antifungal potential. Previous literature reported that phytochemical constituents improved antioxidant performance, thereby enhancing the biological activities described.

Banana (Musa spp.) é caracterizada como um alimento que possui alto valor nutricional e, atualmente, pesquisas sobre seus compostos têm crescido de forma considerável, tendo em vista a presença de atividades antioxidantes e biológicas que o fruto possui. Dessa forma, o objetivo desse artigo é sintetizar e organizar informações sobre os constituintes fitoquímicos, as atividades antioxidantes e as atividades biológicas encontradas em Musa acuminata, Musa balbisiana e Musa paradisíaca e avaliar se tais atividades se relacionam e se há influência de uma sobre a outra. Para tanto, foi feita uma revisão bibliográfica nas plataformas: Google Acadêmico, SciELO, Periódicos Capes, Scientific Eletronic Libary Online (SciELO), Science Direct, PubMed e scopus, por meio dos termos Musa spp, antioxidant, activities biologics e phytochemical intercalados pelo operador booleano AND. O recorte temporal foi de 2017 a 2022 e 28 trabalhos foram selecionados para análise. Em relação à constituição fitoquímica foi possível concluir que as variedades avaliadas são ricas em flavonoides. Para a atividade antioxidante observou-se que Musa spp. apresentam boa performance nos testes antioxidantes do DPPH e FRAP. E para as atividades biológicas as espécies analisadas se destacaram pelo seu potencial como antibacteriano, anticancerígeno e antifúngico. Vários trabalhos revisados apresentaram que os constituintes químicos presentes nas espécies favorecem o bom desempenho antioxidante, esse, por sua vez, influencia positivamente no desempenho das atividades biológicas descritas.

Comparison of the alcohol content obtained in the fermentation of dwarf cavendish bananaand its peel for the purposes of brandy production / Comparação do teor alcóolico obtido na fermentação de banana nanica e de sua casca para fins de produção de aguardente

Brandy is one of the most consumed alcoholic beverages in Brazil, being produced with sweet vegetables, cereals, sugar cane and fruits that have fermentable sugars. This drink is confused with cachaça, from which it is distinguished by the alcoholic degree and typically Brazilian productivity. Banana stands out in Brazil and in the world as the second fruit in productivity, behind only orange, being an accessible raw material for the production of brandy. This processing is advantageous due to the rapid ripening of this fruit, which can often make it impossible to fresh to the consumer. This study aimed to carry out the fermentation through the inoculation of the yeast Saccharomyces cerevisiaeseparately for pulp and skins of Dwarf Cavendish bananas to verify the alcohol content produced. The banana pulp brandy reached an alcohol content of 39,5° GL, within the stipulated by the current legislation, the peel, however, did not allow adequate alcohol levels (7,5° GL). It is concluded that the pulp of Dwarf Cavendish banana is viable for the production of brandy.(AU)

A aguardente é uma das bebidasalcoólicasmais consumidasno Brasil, sendo produzida comvegetais doces, cereais, cana-de-açúcar e frutas que possuemaçúcares fermentescíveis. Esta bebida é confundida com a cachaça, da qual é diferenciadapelo grau alcoólicoe produtividade tipicamente brasileira. Abanana possui destaque no Brasil e no mundo como a segunda fruta em produtividade,atrás apenas da laranja, sendo uma matéria-prima acessível para a produção de aguardenteem razão do processo de amadurecimento rápido,o que acaba muitas vezes por inviabilizar a venda in naturapara o consumidor. Este estudo objetivou realizar a fermentação através da inoculação da levedura Saccharomyces cerevisiaeseparadamente para mostosdapolpa e cascas de bananas nanicapara verificar o teor alcóolico produzido. A aguardente de polpa de bananaatingiu teor alcoólicode 39,5° GL, dentro do estipulado pela legislação vigente, a casca, contudo, não permitiuníveis alcóolicos adequados(7,5°GL). Conclui-se que a polpa da banana nanica é viável para a produção de aguardente.(AU)

Cellulolytic activity of anaerobic facultative fungi from the digestive tract of sheep fed with banana leaf hay / Atividade celulolítica de fungos aeróbios facultativos do trato digestório de ovinos alimentados com feno da folha de bananeira

The addition of cellulolytic fungi, or their enzymes, in diets containing high levels of fiber are promising strategy for improving the performance. In this study, the aims were to select cellulolytic fungi from the digestive tract of sheep fed different concentrations of banana leaf hay. Thirty lambs raised in a feedlot were evaluated, distributed in a completely randomized design, with diets containing 0, 125, 250, 375, or 500 g/Kg of dry matter and six replications. Approximately 15 mL of ruminal fluid and swabs from the rectal ampulla were collected. The cultures were carried out in a medium containing microcrystalline cellulose (C medium). The mycelial fungi isolates were identified through the microculture technique. Among the fungi from the ruminal fluid, 23 isolates corresponded to the genus Aspergillus and three to Paecilomyces spp. Among the isolates from the rectal ampulla, seven were A. spp., and three were P. spp. The A. genus predominated among the isolates from both evaluated sites (p <0.05). Fragments of these fungi were inoculated in triplicate in medium C at 37 °C and the cellulolytic activity index (CAI) was determined after 24, 48, and 72 hours of incubation. There was no difference in the CAI of Aspergillus spp. from animals fed different diets or of different evaluated sites (P > 0.05). However, 22 isolates of Aspergillus spp. and three of Paecilomyces spp. showed a CAI > 1, indicating biotechnological potential for cellulase production. These selected isolates could be selected for the elaboration of microbial additives in ruminant diets.(AU)

A adição de fungos celulolíticos, ou suas enzimas, em dietas contendo elevados teores de fibras são estratégias pro-missoras para melhorar o desempenho. Neste estudo os objetivos foram selecionar fungos celulolíticos do trato digestório de ovinos alimentados com diferentes concentrações do feno da folha da bananeira (FBH). Foram avaliados 30 borregos criados em sistema intensivo, distribuídos em delineamento inteiramente ao acaso, com cinco dietas contendo 0, 125, 250, 375 ou 500 g/KG de matéria seca em seis repetições. Foram coletados aproximadamente 15 mL de fluido ruminal e swabs da ampola retal. Os cultivos foram realizados em meio de cultura contendo celulose microcristalina (meio C). Os fungos micelianos foram identificados após a técnica de microcultivo. Entre os fungos provenientes do fluido ruminal, 23 isolados corresponderam ao gênero Aspergillus e três a Paecilomyces spp.. Foram identificados nas fezes dos animais sete Aspergillus spp. e três Paecilomyces spp.. O gênero Aspergillus predominou entre os isolados de ambos os sítios avaliados (p =0,013). Fragmentos desses fungos foram inoculados em triplicada em meio C a 37 °C e determinou-se o índice de atividade celulolítica(IAC) após 24, 48 e 72 horas de incubação. Não houve diferença entre CAI de isolados de Aspergillus spp. provenientes dos animais em diferentes dietas ou sítios avaliados (P > 0.05). Entretanto, 22 isolados de Aspergillus spp. e três de Paecilomyces spp. apresentaram IAC >1, indicando potencial biotecnológico para produção de celulases.(AU)

Microbiological properties of soils are sensitive to changes provided by organic cultivation of banana 'BRS Princesa' in the semi-arid region

Soil microbiota has a key role in the dynamics of natural and agro-ecosystems and is sensitive to changes in these environments. This study evaluated changes in the microbiological properties of soils under an organic production system of banana 'BRS Princesa' (Musa spp.). The experimental design consisted of completely randomized blocks, with four replications. Treatments consisted of 1) soil cover with green manure and agricultural gypsum at a dose of 2,820 kg ha−1, 2) soil cover with green manure without gypsum application, 3) soil cover with weeds and agricultural gypsum at a dose of 2,820 kg ha−1, 4) soil cover with spontaneous plants without gypsum application, and two controls: 5) soil under native Caatinga and 6) soil under regenerating forest (capoeira). The evaluated properties were ß-glucosidase, arylsulfatase, acid phosphatase, fluorescein diacetate hydrolysis activities (FDA), carbon and phosphorus contents in microbial biomass, basal soil respiration, microbial and metabolic quotients, and arbuscular mycorrhizal fungi spore density. Soil samples were collected from the 0­0.20m depth layer in two seasons. No parameter could distinguish the treatments. Spontaneous plants provided conditions equivalent to those under green manure. Agricultural gypsum application also did not influence the microbial biomass and microbiota activity, in the analyzed soil depth. However, ß-glucosidase and arylsulfatase activities, the carbon content in microbial biomass, and metabolic and microbial quotients were sensitive to land-use changes and could distinguish areas under organic cultivation from those under native vegetation. Therefore, these properties can be considered good indicators for monitoring the quality of these soils. Furthermore, microbial communities of soils under organic cultivation responded with arylsulfatase activity corresponding to that found in soils under regenerating forest, which may indicate that organic management tends to provide the microbiota with a condition similar to that found under situations that are little disturbing to edaphic living.(AU)

Potential nutrient-response curves and sufficiency ranges for ‘Prata-An㒠banana cultivated under two environmental conditions

Proper plant nutrition is critical to increasing the yield of bananas. The objective was to establish the potential nutrient-response curves and sufficiency ranges using the boundary line approach (BLA) and the method proposed by Kenworthy (MK) to assess the nutritional status of ‘Prata-An㒠bananas cultivated under two environmental conditions. The study was carried out using a database comprising leaf nutrient concentrations and banana yields grown at Missão Velha, Ceará, and Ponto Novo, Bahia, Brazil. The reference population consisted of high-yielding plants with yields greater than the mean yield plus 0.5 standard deviation. The database was divided into two datasets. One contained 253 leaf analysis results and a reference population with a mean yield greater than 39.81 t ha−1 yr−1 at Missão Velha. The other contained 147 samples and a reference population with a mean yield greater than 41.69 t ha−1 yr−1 at Ponto Novo. The sufficiency ranges obtained by the BLA for ‘Prata-An㒠banana in Bahia and Ceará, respectively, are: a) for macronutrients (g kg−1): N (19.3-22.0) and (19.9-22.1); P (1.4-2.0) and (1.4-1.6); K (22.6-32.2) and (24.0-31.3); Ca (4.6-6.5) and (5.3-5.8); Mg (1.8-2.6) and (2.1-2.7); S (1.3-2.0) and (1.3-1.5); b) for micronutrients (mg kg−1): B (8.4-13.0) and (13.7-16.4); Cu (5.6-8.4) and (4.4-5.2); Fe (54.2-77.6) and (39.0-55.0); Mn (140.1-222.8) and (64.0-91.0); Zn (13.5-18.3) and (12.4-14.5). The sufficiency ranges obtained by the BLA are more assertive when assessing the nutritional status for ‘Prata-An㒠banana.

Potential nutrient-response curves and sufficiency ranges for ‘Prata-Anã banana cultivated under two environmental conditions

Proper plant nutrition is critical to increasing the yield of bananas. The objective was to establish the potential nutrient-response curves and sufficiency ranges using the boundary line approach (BLA) and the method proposed by Kenworthy (MK) to assess the nutritional status of ‘Prata-Anã bananas cultivated under two environmental conditions. The study was carried out using a database comprising leaf nutrient concentrations and banana yields grown at Missão Velha, Ceará, and Ponto Novo, Bahia, Brazil. The reference population consisted of high-yielding plants with yields greater than the mean yield plus 0.5 standard deviation. The database was divided into two datasets. One contained 253 leaf analysis results and a reference population with a mean yield greater than 39.81 t ha−1 yr−1 at Missão Velha. The other contained 147 samples and a reference population with a mean yield greater than 41.69 t ha−1 yr−1 at Ponto Novo. The sufficiency ranges obtained by the BLA for ‘Prata-Anã banana in Bahia and Ceará, respectively, are: a) for macronutrients (g kg−1): N (19.3-22.0) and (19.9-22.1); P (1.4-2.0) and (1.4-1.6); K (22.6-32.2) and (24.0-31.3); Ca (4.6-6.5) and (5.3-5.8); Mg (1.8-2.6) and (2.1-2.7); S (1.3-2.0) and (1.3-1.5); b) for micronutrients (mg kg−1): B (8.4-13.0) and (13.7-16.4); Cu (5.6-8.4) and (4.4-5.2); Fe (54.2-77.6) and (39.0-55.0); Mn (140.1-222.8) and (64.0-91.0); Zn (13.5-18.3) and (12.4-14.5). The sufficiency ranges obtained by the BLA are more assertive when assessing the nutritional status for ‘Prata-Anã banana.(AU)

Optimization of banana plantlets acclimatization by hydroponic cultivation

Acclimatization is the final phase of banana plantlet production by micropropagation. In this phase, the plantlets are grown in a protected environment with high demand for water, fertilizer and labor, until reaching the standard size and quality required for marketing. The aim of this study was to evaluate the possibility of reducing these costs and the duration of the acclimatization phase, using the hydroponic cultivation technique as compared to the conventional system. ‘Prata–An㒠banana plantlet performance was measured for both systems under both tropical summer and winter conditions, based upon water and nutrient use efficiency and growing time of the plantlets. In both seasons, the plantlets produced hydroponically presented faster growth of both the shoot and root systems. There was a reduction of 12 days to reach the transplantation point, a significant reduction in plantlet production cost (fertilizers + water) and improved efficiency in space use with an expressive increase in the number of plantlets produced per square meter.

Optimization of banana plantlets acclimatization by hydroponic cultivation

Acclimatization is the final phase of banana plantlet production by micropropagation. In this phase, the plantlets are grown in a protected environment with high demand for water, fertilizer and labor, until reaching the standard size and quality required for marketing. The aim of this study was to evaluate the possibility of reducing these costs and the duration of the acclimatization phase, using the hydroponic cultivation technique as compared to the conventional system. ‘Prata–Anã banana plantlet performance was measured for both systems under both tropical summer and winter conditions, based upon water and nutrient use efficiency and growing time of the plantlets. In both seasons, the plantlets produced hydroponically presented faster growth of both the shoot and root systems. There was a reduction of 12 days to reach the transplantation point, a significant reduction in plantlet production cost (fertilizers + water) and improved efficiency in space use with an expressive increase in the number of plantlets produced per square meter.(AU)

Growth and production of 'Prata Anã Gorutuba' banana under different planting densities

Increased planting density, different types of thinning management, and the use of beneficial microorganisms have been adopted as strategic tools in the effort to increase crop yields in a sustainable manner. This investigation evaluated the growth and production of ‘Prata Anã Gorutuba’ banana ( Musa spp., AAB) under different plant densities during two production cycles in the semiarid region of Minas Gerais, Brazil. Micropropagated plantlets were also inoculated with endophytic bacteria and cultivated as part of two mat management strategies. Planting density varied between 1,680 and 3,920 plant ha–1. During the experiment, water was less than optimal because of rationing due to drought in the region. Under field conditions, there was no difference between bacterially inoculated and non-inoculated plants. The dense planting system increased the crop cycle. The highest-density planting treatment (3,920 plant ha–1) resulted in the highest yield in the first cycle, but no net income was observed. However, the high-density planting treatments resulted in the highest yields and gross incomes in the second production cycle. Management with thinning of plants up to 10-months-old and thinning up to harvest associated with high planting density increased the length of the production cycles. Although the water restriction experienced in the first and second crop cycles, the vegetative and productive traits of ‘Prata Anã Gorutuba’ banana did not compromise the objectives of this study.

Growth and production of 'Prata Anã Gorutuba' banana under different planting densities

Increased planting density, different types of thinning management, and the use of beneficial microorganisms have been adopted as strategic tools in the effort to increase crop yields in a sustainable manner. This investigation evaluated the growth and production of ‘Prata Anã Gorutuba banana ( Musa spp., AAB) under different plant densities during two production cycles in the semiarid region of Minas Gerais, Brazil. Micropropagated plantlets were also inoculated with endophytic bacteria and cultivated as part of two mat management strategies. Planting density varied between 1,680 and 3,920 plant ha–1. During the experiment, water was less than optimal because of rationing due to drought in the region. Under field conditions, there was no difference between bacterially inoculated and non-inoculated plants. The dense planting system increased the crop cycle. The highest-density planting treatment (3,920 plant ha–1) resulted in the highest yield in the first cycle, but no net income was observed. However, the high-density planting treatments resulted in the highest yields and gross incomes in the second production cycle. Management with thinning of plants up to 10-months-old and thinning up to harvest associated with high planting density increased the length of the production cycles. Although the water restriction experienced in the first and second crop cycles, the vegetative and productive traits of ‘Prata Anã Gorutuba banana did not compromise the objectives of this study.(AU)

Optimization of banana plantlets acclimatization by hydroponic cultivation

ABSTRACT: Acclimatization is the final phase of banana plantlet production by micropropagation. In this phase, the plantlets are grown in a protected environment with high demand for water, fertilizer and labor, until reaching the standard size and quality required for marketing. The aim of this study was to evaluate the possibility of reducing these costs and the duration of the acclimatization phase, using the hydroponic cultivation technique as compared to the conventional system. PrataAnã banana plantlet performance was measured for both systems under both tropical summer and winter conditions, based upon water and nutrient use efficiency and growing time of the plantlets. In both seasons, the plantlets produced hydroponically presented faster growth of both the shoot and root systems. There was a reduction of 12 days to reach the transplantation point, a significant reduction in plantlet production cost (fertilizers + water) and improved efficiency in space use with an expressive increase in the number of plantlets produced per square meter.

Agronomic performance of 'BRS Princesa' banana under fertigation and mulching / Desempenho agronômico da bananeira 'BRS Princesa' sob fertirrigação e cobertura morta

Banana is one of the most produced fruit crops in Brazil and has great economic, social and nutritional importance. Factors such as water availability and well-managed fertilization are fundamental to achieving profitable yields. The aim of this study was to examine the agronomic performance and water use efficiency of 'BRS Princesa' banana under combinations of irrigation systems, fertilization methods and mulching, during three production cycles. The experiment was laid out in a randomized-block design with four replicates. Treatments consisted of a combination of the localized irrigation system, fertilization strategy and mulching, as follows: Drip irrigation with manual fertilization; Micro-sprinkler irrigation with manual fertilization; Drip fertigation with mulch; Drip fertigation without mulch; Micro-sprinkler fertigation with mulch; and Microsprinkler fertigation without mulch. The plants were irrigated every three days, using three 4-L h-1 drippers per plant or a 64-L h-1 micro-sprinkler for every four plants. The following variables were analyzed: number of leaves, plant height, pseudostem circumference, number of hands, hand yield, water and nutrient (nitrogen and potassium) use efficiency and earliness. The micro-sprinkler system provided a greater pseudostem circumference without the presence of mulch. Plants under micro-sprinkler fertigation with mulching grew taller. The banana yield was higher when fertigation was used than with manual fertilization, regardless of the irrigation system. Plants grown in mulched soil were more productive than those grown in bare soil. The use of mulch increased water, nitrogen and potassium use efficiency when compared with cultivation in bare soil. Drip-irrigated plants showed earlier production than those irrigated with micro-sprinklers.(AU)

A bananeira é sendo uma das fruteiras mais produzidas no Brasil e possui grande importância econômica, social e alimentícia. Fatores como disponibilidade de água e adubação bem manejados são fundamentais para obtenção de produtividades rentáveis. Objetivou-se com este trabalho, avaliar o desempenho agronômico e a eficiência no uso da água da bananeira 'BRS Princesa' sob combinações de sistemas de irrigação, métodos de adubação e cobertura morta, durante três ciclos de produção. O delineamento experimental foi o de blocos casualizados, com quatro repetições. Os tratamentos consistiram na combinação do sistema de irrigação localizada, estratégia de adubação e uso de cobertura morta: a) Irrigação por gotejamento com adubação manual; b) Irrigação por microaspersão com adubação manual; c) Fertirrigação por gotejamento com cobertura morta; d) Fertirrigação por gotejamento sem cobertura morta; e) Fertirrigação por microaspersão com cobertura morta e f) Fertirrigação por microaspersão sem cobertura morta. As irrigações ocorreram a cada três dias, utilizando-se três gotejadores de 4 L h-1 por planta ou um microaspersor de 64 L h-1 para cada quatro plantas. As variáveis estudadas foram: número de folhas, altura da planta, perímetro do pseudocaule, número de pencas, produtividade, eficiência no uso da água e de nutrientes (nitrogênio e potássio) e precocidade. A microaspersão proporcionou maior circunferência do pseudocaule sem a presença da cobertura do solo. Plantas sob fertirrigação por microaspersão com cobertura do solo expressam maiores alturas. A produtividade da bananeira foi maior quando utilizada a fertirrigação em detrimento da adubação manual, independente do sistema de irrigação. As plantas cultivadas em solo coberto foram mais produtivas que aquelas cultivadas em solo descoberto. O uso da cobertura morta elevou a eficiência do uso da água, de nitrogênio e de potássio em relação ao cultivo em solo descoberto. As plantas irrigadas por gotejamento apresentaram produção mais precoce que irrigadas por microaspersão.(AU)

Potential nutrient-response curves and sufficiency ranges for Prata-Anã banana cultivated under two environmental conditions

ABSTRACT: Proper plant nutrition is critical to increasing the yield of bananas. The objective was to establish the potential nutrient-response curves and sufficiency ranges using the boundary line approach (BLA) and the method proposed by Kenworthy (MK) to assess the nutritional status of Prata-Anã bananas cultivated under two environmental conditions. The study was carried out using a database comprising leaf nutrient concentrations and banana yields grown at Missão Velha, Ceará, and Ponto Novo, Bahia, Brazil. The reference population consisted of high-yielding plants with yields greater than the mean yield plus 0.5 standard deviation. The database was divided into two datasets. One contained 253 leaf analysis results and a reference population with a mean yield greater than 39.81 t ha1 yr1 at Missão Velha. The other contained 147 samples and a reference population with a mean yield greater than 41.69 t ha1 yr1 at Ponto Novo. The sufficiency ranges obtained by the BLA for Prata-Anã banana in Bahia and Ceará, respectively, are: a) for macronutrients (g kg1): N (19.3-22.0) and (19.9-22.1); P (1.4-2.0) and (1.4-1.6); K (22.6-32.2) and (24.0-31.3); Ca (4.6-6.5) and (5.3-5.8); Mg (1.8-2.6) and (2.1-2.7); S (1.3-2.0) and (1.3-1.5); b) for micronutrients (mg kg1): B (8.4-13.0) and (13.7-16.4); Cu (5.6-8.4) and (4.4-5.2); Fe (54.2-77.6) and (39.0-55.0); Mn (140.1-222.8) and (64.0-91.0); Zn (13.5-18.3) and (12.4-14.5). The sufficiency ranges obtained by the BLA are more assertive when assessing the nutritional status for Prata-Anã banana.

Effects of different banana crop residue hays on ensiling BRS capiaçu grass on fermentation profile, aerobic stability and nutritional value of silage / Efeitos de diferentes fenos de resíduo da bananicultura na ensilagem do capim-BRS capiaçu sobre o perfil de fermentativo, estabilidade aeróbia e valor nutricional da silagem

The objective of this study was to evaluate the BRS capiaçu grass silage combined with different hays of banana crop residue on fermentation profile, aerobic stability and nutritional value. The treatments consisted of elephant grass cv. BRS capiaçu (Pennisetum purpureum Schum.) ensiled with 37.44% banana peel hay, 36.06% banana pseudostem hay and 37.00% banana leaf hay, on a dry matter (DM) basis and control silage (no additive). The experimental design used was completely randomized, with five treatments and five replicates. Forage was collected when it reached 3.5 meters in height (90 days). Experimental PVC silos of known weight, 50 cm long, 10 cm diameter, were used for silage making. For all treatments, silage aerobic stability breakdown started after 64 hours exposure to air. The BRS capiaçu grass control silage or silage combined with pseudostem hay (mean of 73.15 kg t GM-1) presented effluent losses 40.46% higher than those observed for BRS capiaçu grass silage + banana leaf hay and 69.17% in relation the BRS capiaçu grass silage + banana peel hay. The inclusion of banana crop residue (hay) when ensiling BRS capiaçu grass decreased 13.93% gas losses compared to the control silage (mean of 3.11% DM). Higher values of total digestible nutrients, metabolizable energy and digestible energy content was found in BRS capiaçu grass silage + with banana peel hay. The greater in vitro dry matter digestibility and in vitro neutral detergent fiber digestibility was observed for BRS capiaçu grass silage combined with pseudostem hay. The inclusion of 37.44% banana peel hay improves the fermentation profile and aerobic stability of BRS capiaçu grass silage.(AU)

Ojetivou-se por meio desta pesquisa avaliar a silagem de capim-BRS capiaçu associada com diferentes fenos de resíduos da cultura da banana sobre o perfil fermentativo, estabilidade aeróbia e valor nutricional. Os tratamentos consistiram de capim-elefante cv. BRS capiaçu (Pennisetum purpureum Schum.) ensilado com 37,44% de feno de casca de banana, 36,06% de feno de pseudocaule de banana e 37,00% de feno de folha de bananeira, à base da matéria seca (MS), e a silagem controle (sem aditivo). O delineamento experimental utilizado foi inteiramente casualizado com cinco tratamentos e cinco repetições. A forragem foi coletada quando atingiu 3,5 metros de altura (90 dias). Para a produção da silagem, foram utilizados silos experimentais de PVC de peso conhecido, com 50 cm de comprimento e 10 cm de diâmetro. Para todos os tratamentos, a quebra da estabilidade aeróbia da silagem iniciou a partir de 64 horas de exposição ao ar. A silagem de BRS capiaçu controle ou associada ao feno de pseudocaule (média de 73,15 kg t MN-1) apresentaram perdas de efluentes 40,46% maiores do que as observadas para a silagem de BRS capiaçu associada ao feno de folha de bananeira e 69,17% em relação à silagem de BRS capiaçu associada à casca de banana. A inclusão dos resíduos da cultura da banana (feno) na ensilagem do capim- BRS capiaçu diminuiu 13,93% as perdas por gases em comparação com a silagem controle (média de 3,11% da matéria seca - MS). Maiores teores de nutrientes totais digestíveis, energia metabolizável e energia digestível foram verificadas na silagem de BRS capiaçu associada à casca de banana. A maior digestibilidade in vitro da matéria seca e da fibra em detergente neutro foi verificada na silagem de BRS capiaçu associada com feno de pseudocaule. A inclusão de 37,44% de feno de casca de banana melhora o perfil fermentativo e a estabilidade aeróbia da silagem de capim- BRS capiaçu.(AU)

Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense / Bioensaio em discos de rizomas de bananeiras para a seleção de bactérias antagonistas a Fusarium oxysporum f. sp. cubense

Detached plant organs are alternative materials to in vitro tests for selecting biocontrol agents. On the other hand, the use of scales to quantify injured areas can generate inconsistent results. Rhizospheric and endophytic bacteria were selected as growth inhibitors of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Panama disease of banana. For this, rhizome discs were treated with 200 µL of antagonist suspension (109 CFU mL−¹) and inoculated with the pathogen. The material was placed in plastic gerbox boxes and incubated in BOD at 25 °C for 12 days. Afterward, the area of rhizome discs (mm²) colonized by Foc was quantified by digital images. The assay was set up in a completely randomized design, with four replications and three discs per replication. The control consisted of untreated and inoculated discs. The results showed the efficiency of this method in selecting the biological control agent, as the 26 isolates were group into five different clusters, with isolates belonging to four of these groups (from '1' to '4') being able to reduce Foc colonization. Isolates 520EB, 993EB, and 531EB had the highest potential for inhibition, with areas of 343.3, 344.1, 364.8 mm², respectively, promoting inhibition ranging from 53 to 56 % of the colonized area compared to the control (782.6 mm²).

O emprego de órgãos vegetais destacados é uma alternativa aos testes in vitro para seleção de agentes de biocontrole. Por outro lado, o uso de escalas para quantificação de área lesionada pode gerar resultados inconsistentes. Bactérias rizosféricas e endofíticas foram selecionadas como inibidoras do crescimento de Fusarium oxysporum f. sp. cubense (Foc), agente causal do mal-do-Panamá da bananeira. Para tanto, discos de rizoma foram tratados com 200 µL de suspensão dos antagonistas (109 UFC mL-¹) e inoculados com patógeno. O material foi disposto em caixas plásticas tipo gerbox e incubado em BOD a 25 ºC, por doze dias. Após, a área dos discos de rizoma (mm²) colonizada por Foc dos discos foi quantificada por meio de imagens digitais. O ensaio foi montado em delineamento inteiramente casualizado, com quatro repetições e três discos por repetição. Discos não tratados e inoculados compuseram o controle. Com base nos resultados obtidos, foi possível verificar a eficiência do método na seleção de agente de controle biológico, uma vez que foi possível o agrupamento dos 26 isolados utilizados em cinco clústeres distintos, sendo que isolados pertencentes a quatro destes grupos (do '1' a '4') foram capazes de proporcionar a redução da colonização por Foc. Os isolados com maior potencial de inibição foram520EB, 993EB e 531EB com 343,3, 344,1, 364,8 mm2, respectivamente, promovendo inibição variando de 53 a 56 % da área colonizada quando comparados com ao controle (782,6 mm²).

Bioassay on banana rhizome discs for selecting bacteria antagonistic to Fusarium oxysporum f. sp. cubense / Bioensaio em discos de rizomas de bananeiras para a seleção de bactérias antagonistas a Fusarium oxysporum f. sp. cubense

Detached plant organs are alternative materials to in vitro tests for selecting biocontrol agents. On the other hand, the use of scales to quantify injured areas can generate inconsistent results. Rhizospheric and endophytic bacteria were selected as growth inhibitors of Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Panama disease of banana. For this, rhizome discs were treated with 200 µL of antagonist suspension (109 CFU mL−¹) and inoculated with the pathogen. The material was placed in plastic gerbox boxes and incubated in BOD at 25 °C for 12 days. Afterward, the area of rhizome discs (mm²) colonized by Foc was quantified by digital images. The assay was set up in a completely randomized design, with four replications and three discs per replication. The control consisted of untreated and inoculated discs. The results showed the efficiency of this method in selecting the biological control agent, as the 26 isolates were group into five different clusters, with isolates belonging to four of these groups (from '1' to '4') being able to reduce Foc colonization. Isolates 520EB, 993EB, and 531EB had the highest potential for inhibition, with areas of 343.3, 344.1, 364.8 mm², respectively, promoting inhibition ranging from 53 to 56 % of the colonized area compared to the control (782.6 mm²).(AU)

O emprego de órgãos vegetais destacados é uma alternativa aos testes in vitro para seleção de agentes de biocontrole. Por outro lado, o uso de escalas para quantificação de área lesionada pode gerar resultados inconsistentes. Bactérias rizosféricas e endofíticas foram selecionadas como inibidoras do crescimento de Fusarium oxysporum f. sp. cubense (Foc), agente causal do mal-do-Panamá da bananeira. Para tanto, discos de rizoma foram tratados com 200 µL de suspensão dos antagonistas (109 UFC mL-¹) e inoculados com patógeno. O material foi disposto em caixas plásticas tipo gerbox e incubado em BOD a 25 ºC, por doze dias. Após, a área dos discos de rizoma (mm²) colonizada por Foc dos discos foi quantificada por meio de imagens digitais. O ensaio foi montado em delineamento inteiramente casualizado, com quatro repetições e três discos por repetição. Discos não tratados e inoculados compuseram o controle. Com base nos resultados obtidos, foi possível verificar a eficiência do método na seleção de agente de controle biológico, uma vez que foi possível o agrupamento dos 26 isolados utilizados em cinco clústeres distintos, sendo que isolados pertencentes a quatro destes grupos (do '1' a '4') foram capazes de proporcionar a redução da colonização por Foc. Os isolados com maior potencial de inibição foram520EB, 993EB e 531EB com 343,3, 344,1, 364,8 mm2, respectivamente, promovendo inibição variando de 53 a 56 % da área colonizada quando comparados com ao controle (782,6 mm²).(AU)

Physicochemical characteristics of unripe and ripe banana "FHIA 18" submitted to phosphorus fertilizer over three production cycles / Perfil físico-químico de frutos verdes e maduros da bananeira "FHIA 18" submetida à adubação fosfatada em três ciclos produtivos

The banana fruits of "FHIA 18" have sensory properties like the "Prata" ones. However, there is no information regarding to phosphorus (P) requirement of this hybrid and its effects on postharvest quality. This study aims to evaluate the physicochemical properties of "FHIA 18" banana fruits submitted to different phosphorus levels under the edaphoclimatic conditions of the municipality of São Manuel, state of São Paulo, during three successive production cycles. Therefore, different levels of Phosphorus (0 [0%], 20 [50%)], 30 [75%], 40 [100%], 50 [125%] and 60 kg ha-1 year-1 P2O5 [150%]) were applied during three production cycles. A completely randomized design and split plot structure was used, the plots consisted of six phosphorus fertilizers doses and three production cycles (2014, 2015 and 2016) in sub plots. For unripe fruits, we evaluated the contents of dry matter, starch, total sugars, reducing sugars and phosphorus. For ripe fruits, we analyzed firmness, peel colour descriptors, total titratable acidity, total soluble solids, maturity index, total sugars and reducing sugars. Results showed that P fertilizer provided an increase in P content in unripe fruits until the application of 100% of the recommended dose; besides an increase in the reducing sugar contents until the application of 61% over the first production cycle. Results also indicated that applying lower phosphorus doses (˂100% of the recommended dose) promoted lower total titratable acidity and higher maturity index (52-53% of recommendation) in ripe fruits. Regardless of P2O5 dose, the contents of dry matter, starch and phosphorus increased in unripe fruits from first to third crop cycle, as well as the firmness, total soluble solids contents, maturity index and sugar content in ripe fruits.

Os frutos da bananeira "FHIA 18" possuem características sensoriais semelhantes à banana "Prata", porém, não existem informações sobre as reais necessidades em fósforo (P) deste híbrido e seus efeitos na produção e qualidade pós-colheita. O objetivo deste trabalho foi avaliar o perfil físico-químico dos frutos da bananeira "FHIA 18" submetida à níveis de adubação fosfatada, em São Manuel-SP, durante três ciclos sucessivos. Os níveis de adubação com P2O5 aplicados foram: sem adubação com P (0%); 20 kg ha-1 ano-1 (50%); 30 kg ha-1 ano-1 (75%); 40 kg ha-1 ano-1 (100%); 50 kg ha-1 ano-1 (125%); e 60 kg ha-1 ano-1 (150%). Utilizou-se o delineamento inteiramente casualizado, em arranjo de parcelas subdivididas, sendo as parcelas compostas pelos seis níveis de adubação com fósforo e as subparcelas pelos três ciclos de produção (2014, 2015 e 2016). Foram avaliados nos frutos verdes os teores de matéria seca, amido, açúcares totais, açúcares redutores e de fósforo. Nos frutos maduros avaliou-se a firmeza, os descritores de coloração da casca, acidez titulável total, sólidos solúveis totais, índice de maturação, açúcares totais e açúcares redutores. A adubação fosfatada proporcionou aumento nos teores de fósforo nos frutos verdes até a aplicação de 100% da recomendação e aumento dos teores de açúcares redutores até a aplicação de 61% no primeiro ciclo de produção. Quanto aos frutos maduros, a utilização de fósforo em doses inferiores às recomendadas (100%) promoveu menor acidez titulável total e maior índice de maturação (52-53% da recomendação). Por outro lado, independente da dose de P2O5 utilizada, os teores de matéria seca, amido e fósforo, nos frutos verdes, e a firmeza, teores de sólidos solúveis totais, índice de maturação e teores de açúcares nos frutos maduros, aumentam do primeiro ao terceiro ciclo de produção.

Physicochemical characteristics of unripe and ripe banana "FHIA 18" submitted to phosphorus fertilizer over three production cycles / Perfil físico-químico de frutos verdes e maduros da bananeira "FHIA 18" submetida à adubação fosfatada em três ciclos produtivos

The banana fruits of "FHIA 18" have sensory properties like the "Prata" ones. However, there is no information regarding to phosphorus (P) requirement of this hybrid and its effects on postharvest quality. This study aims to evaluate the physicochemical properties of "FHIA 18" banana fruits submitted to different phosphorus levels under the edaphoclimatic conditions of the municipality of São Manuel, state of São Paulo, during three successive production cycles. Therefore, different levels of Phosphorus (0 [0%], 20 [50%)], 30 [75%], 40 [100%], 50 [125%] and 60 kg ha-1 year-1 P2O5 [150%]) were applied during three production cycles. A completely randomized design and split plot structure was used, the plots consisted of six phosphorus fertilizers doses and three production cycles (2014, 2015 and 2016) in sub plots. For unripe fruits, we evaluated the contents of dry matter, starch, total sugars, reducing sugars and phosphorus. For ripe fruits, we analyzed firmness, peel colour descriptors, total titratable acidity, total soluble solids, maturity index, total sugars and reducing sugars. Results showed that P fertilizer provided an increase in P content in unripe fruits until the application of 100% of the recommended dose; besides an increase in the reducing sugar contents until the application of 61% over the first production cycle. Results also indicated that applying lower phosphorus doses (˂100% of the recommended dose) promoted lower total titratable acidity and higher maturity index (52-53% of recommendation) in ripe fruits. Regardless of P2O5 dose, the contents of dry matter, starch and phosphorus increased in unripe fruits from first to third crop cycle, as well as the firmness, total soluble solids contents, maturity index and sugar content in ripe fruits.(AU)

Os frutos da bananeira "FHIA 18" possuem características sensoriais semelhantes à banana "Prata", porém, não existem informações sobre as reais necessidades em fósforo (P) deste híbrido e seus efeitos na produção e qualidade pós-colheita. O objetivo deste trabalho foi avaliar o perfil físico-químico dos frutos da bananeira "FHIA 18" submetida à níveis de adubação fosfatada, em São Manuel-SP, durante três ciclos sucessivos. Os níveis de adubação com P2O5 aplicados foram: sem adubação com P (0%); 20 kg ha-1 ano-1 (50%); 30 kg ha-1 ano-1 (75%); 40 kg ha-1 ano-1 (100%); 50 kg ha-1 ano-1 (125%); e 60 kg ha-1 ano-1 (150%). Utilizou-se o delineamento inteiramente casualizado, em arranjo de parcelas subdivididas, sendo as parcelas compostas pelos seis níveis de adubação com fósforo e as subparcelas pelos três ciclos de produção (2014, 2015 e 2016). Foram avaliados nos frutos verdes os teores de matéria seca, amido, açúcares totais, açúcares redutores e de fósforo. Nos frutos maduros avaliou-se a firmeza, os descritores de coloração da casca, acidez titulável total, sólidos solúveis totais, índice de maturação, açúcares totais e açúcares redutores. A adubação fosfatada proporcionou aumento nos teores de fósforo nos frutos verdes até a aplicação de 100% da recomendação e aumento dos teores de açúcares redutores até a aplicação de 61% no primeiro ciclo de produção. Quanto aos frutos maduros, a utilização de fósforo em doses inferiores às recomendadas (100%) promoveu menor acidez titulável total e maior índice de maturação (52-53% da recomendação). Por outro lado, independente da dose de P2O5 utilizada, os teores de matéria seca, amido e fósforo, nos frutos verdes, e a firmeza, teores de sólidos solúveis totais, índice de maturação e teores de açúcares nos frutos maduros, aumentam do primeiro ao terceiro ciclo de produção.(AU)

Soil-water-plant relationship and fruit yield under partial root-zone drying irrigation on banana crop

Climatic uncertainties have compromised water resources, which influences irrigated agriculture. Irrigation is an important alternative to mitigate the effects of water scarcity. Partial root-zone drying (PRD) is a rational use of irrigation water. This study aimed to evaluate PRD for irrigation management of banana crop, cv BRS Princesa, under semi-arid conditions in Brazil. A field experiment was carried out with five treatments: one control (full irrigation) and four with 50 % reduction of water irrigation depth (WID) and frequencies alternating the side to be irrigated of the plant row of 0, 7, 14 and 21 days. Stomatal conductance, growth, yield and soil water status were evaluated. Results indicated that banana crop cv. BRS Princesa shows shoot physiological regulation for 50 % reduction of soil water and vapor pressure deficit between 1.92 and 2.25 kPa. Decrease in yields of treatments with WID reduction compared to full irrigation is due to the reduction of root uptake rate, which is related to vapor pressure deficit and soil water availability. Partial root-zone drying saves 50 % of irrigation water and increases water use efficiency of banana cv. BRS Princesa crop by 78 %.

Soil-water-plant relationship and fruit yield under partial root-zone drying irrigation on banana crop

Climatic uncertainties have compromised water resources, which influences irrigated agriculture. Irrigation is an important alternative to mitigate the effects of water scarcity. Partial root-zone drying (PRD) is a rational use of irrigation water. This study aimed to evaluate PRD for irrigation management of banana crop, cv BRS Princesa, under semi-arid conditions in Brazil. A field experiment was carried out with five treatments: one control (full irrigation) and four with 50 % reduction of water irrigation depth (WID) and frequencies alternating the side to be irrigated of the plant row of 0, 7, 14 and 21 days. Stomatal conductance, growth, yield and soil water status were evaluated. Results indicated that banana crop cv. BRS Princesa shows shoot physiological regulation for 50 % reduction of soil water and vapor pressure deficit between 1.92 and 2.25 kPa. Decrease in yields of treatments with WID reduction compared to full irrigation is due to the reduction of root uptake rate, which is related to vapor pressure deficit and soil water availability. Partial root-zone drying saves 50 % of irrigation water and increases water use efficiency of banana cv. BRS Princesa crop by 78 %.(AU)