Nutritional, physiological, hematological, and biochemical responses of lambs fed increasing levels of Pornunça silage

ABSTRACT: Pornunça (Manihot sp.) is a potential forage to feed ruminants in drylands worldwide; however, evaluations of animal diets are necessary. This study assessed intake and digestibility of dry matter and nutrients, physiological responses, ingestive behavior, water intake, as well as hematological and biochemical responses of lambs fed diets containing increasing levels of Pornunça silage (PS) replacing Tifton-85 bermudagrass hay (Cynodon spp.). Treatments consisted of 0, 33, 66, and 100 % PS considering the roughage portion of the diet (% dry matter DM). The experimental design was completely randomized with six replicates. Cyanide acid (HCN) levels in fresh Pornunça leaves were 207.7 mg kg1 DM and 76.78 mg kg1 DM in PS. Intake of DM and crude protein (CP), and neutral detergent fiber corrected for ash and protein (NDFap) increased linearly with increasing levels of PS in the diet. Digestibility of DM, NDF, and CP were higher with increasing levels of PS. Idle time decreased linearly, while water balance, water intake via food, and total water in feces increased with PS. Nitrogen balance, physiological, and hematological responses were not influenced by treatments, while the final alanine aminotransferase increased for lambs fed all diets evaluated. The PS up to 100 % of the roughage portion increased DM and nutrient intake and digestibility without altering physiological responses and the hematological and biochemical parameters, resulting in greater water intake via food.

Agromorphological, yield and quality characteristics of two populations of alfalfa developed by mass selection / Características agromorfológicas, produtivas e de qualidade de duas populações de alfafa desenvolvidas por seleção massal

ABSTRACT: This study was designed to test the two alfalfa advanced populations (L-1737 and L-1738) with four control cultivars (Bilensoy-80, Gözlü, Savaş and Plato) for agromorphological, yield and quality properties under irrigated conditions in two various locations in Ankara, Turkey in the years of 2013, 2014, and 2015. The experiments were arranged in a randomized block design with four replications. Results of the study showed that there were statistically significant differences in plant height and stem diameter as well as stem number among the study materials. Statistically significant differences were observed for green forage yield (P < 0.05), while not in dry matter yield among the study materials. The average green forage and dry matter yields were reported as 67.0 t ha-1 and 13. 0 t ha-1, respectively. Green forage and dry matter yields in the advanced populations of L-1737 and L-1738 were 69.2 and 67.4 t ha-1; 13.7 and 13.6 t ha-1, respectively. Those yield values were 6.5 and 3.7%; 4.3 and 3.4% higher in green forage and dry matter yields of the advanced populations of L-1737 and L-1738 compared to the control cultivar Bilensoy-80, respectively. These increases in green forage and dry matter yields were due to the effect of the mass selection method. Statistically significant differences were observed for acid detergent fiber, neutral detergent fiber, and relative feeding value among the study materials, except crude protein contents. It was concluded that the two advanced populations had good adaptation ability, high yield and good quality performance under irrigated conditions and could be used as commercial cultivars.

RESUMO: Este estudo foi desenhado para testar duas populações avançadas de alfafa (L-1737 e L-1738) com quatro cultivares controle (Bilensoy-80, Gözlü, Savaş e Plato) para propriedades agromorfológicas, produtividade e qualidade sob condições irrigadas em dois locais diferentes em Ancara, Turquia, nos anos de 2013, 2014 e 2015. Os experimentos foram dispostos em delineamento de blocos casualizados com quatro repetições. Os resultados do estudo mostraram que houveram diferenças estatisticamente significativas na altura das plantas e diâmetro do colmo, bem como no número de colmos entre os materiais avaliados. Foram observadas diferenças estatisticamente significativas para a produtividade de forragem verde (P < 0.05), enquanto não foram observadas diferenças na produção de matéria seca entre os materiais estudados. As produtividades médias de forragem verde e matéria seca foram de 67.0 t ha-1 e 13.0 t ha-1, respectivamente. Os rendimentos de forragem verde e matéria seca nas populações avançadas de L-1737 e L-1738 foram 69.2 e 67.4 t ha-1; 13.7 e 13.6 t ha-1, respectivamente. Esses valores de rendimento foram 6.5 e 3.7%; 4.3 e 3.4% maiores em rendimentos de forragem verde e matéria seca das populações avançadas de L-1737 e L-1738 em relação à cultivar controle Bilensoy-80, respectivamente. Esses aumentos nos rendimentos de forragem verde e matéria seca foram devidos ao efeito do método de seleção massal. Diferenças, estatisticamente significativas, foram observadas para fibra em detergente ácido, fibra em detergente neutro e valor alimentar relativo entre os materiais estudados, exceto nos teores de proteína bruta. Concluiu-se que as duas populações avançadas apresentaram boa capacidade de adaptação, alta produtividade e bom desempenho em condições irrigadas, podendo ser utilizadas como cultivares comerciais.

Genetic divergence of cassava genotypes from Petrolina

This study aimed to evaluate cassava genotypes to identify the ones with the best nutritional values for ruminant diets. Nine genotypes were used: Amansa Burro (BGM 549), Aramaris (BGM 116), Brasília, Cambadinha (BRS Guaíra), Curvelinha, Engana Ladrão (BGM 1269), Trouxinha (BGM 1468), BRS Gema de Ovo, and BRS Dourada. A completely randomized block experimental design with nine treatments (genotypes) and three blocks (replicates) were employed. The roots were analyzed for dry matter production (DMP) in tonnes per hectare, crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), starch, and in vitro dry matter digestibility (IVDMD). The multivariate analysis was carried out using Ward's hierarchical agglomerative clustering based on Mahalanobis distance. The groups formed were evaluated by Scott-Knott test. The group formed by genotype Engana Ladrão was chosen as the best one for having the highest DMP t ha-1 and IVDMD values and the lowest NDF and ADF contents.(AU)

Composição de cana-de-açúcar para alimentação animal em função da aplicação de bactérias diazotróficas / Composition of sugarcane for animal feeding with the application of diazotrophic bacteria

A cana-de-açúcar pode ser empregada na alimentação animal devido ao seu alto rendimento de matéria seca. No entanto, há muitas recomendações para evitar o emprego como alimento para animais de bom potencial genético, pois apresenta baixo teor proteico e de minerais, bem como e fibra de baixa qualidade. Desta forma, o objetivo deste trabalho foi avaliar os efeitos da aplicação de uma mistura de cinco bactérias diazotróficas (Gluconacetobacter diazotrophicus; Herbaspirillum seropedicae; Herbaspirillum rubrisubalbicans; Paraburkholderia tropica; Nitrospirillum amazonense) e da adubação nitrogenada sobre a produção, maturação e composição químico-bromatológica de cinco variedades de cana-de-açúcar com potencial para serem empregadas na alimentação animal. Os tratamentos foram formados conforme a combinação dos cinco genótipos de cana-de-açúcar (RB867515, RB966928, RB106814, RB106818 e RB106819) com quatro condições de inoculação e adubação nitrogenada em cobertura. Os teores de sólidos solúveis totais foram determinados dos 180 dias após o transplante até a colheita do experimento, onde foi avaliada a produtividade e a amostragem de material foi coletada para as avaliações químico-bromatológicas: matéria seca, nitrogênio acumulado, proteína bruta, fibra em detergente neutro, fibra em detergente ácido, holocelulose, celulose, hemicelulose, lignina, extrativos totais e cinzas. De acordo com os resultados deste estudo, conclui-se que a aplicação de bactérias diazotróficas de forma isolada ou em conjunto com a adubação nitrogenada, aumenta a produtividade e os sólidos solúveis totais, antecipando a maturação, além de melhorar a composição químico-bromatológica das plantas de cana-de-açúcar.

Sugarcane can be used in animal feed due to its high dry matter yield. However, there are many recommendations to avoid its use as food for animals with good genetic potential, since it has a low contents of protein and minerals, as well as fiber of low quality. Therefore, this work aimed to evaluate the effects of applying a mixture of five diazotrophic bacteria (Gluconacetobacter diazotrophicus; Herbaspirillum seropedicae; Herbaspirillum rubrisubalbicans; Paraburkholderia tropica; Nitrospirillum amazonense) and nitrogen fertilization on the production, maturation, and chemical-bromatological composition of five sugarcane varieties with potential to be used in animal feed. The treatments were formed according to the combination of the five sugarcane genotypes (RB867515, RB966928, RB106814, RB106818, and RB106819) with four conditions of inoculation and nitrogen fertilization in coverage. The levels of total soluble solids were determined from 180 days after transplantation until the harvest of the experiment, where productivity was evaluated and the sample material was collected for chemical-bromatological evaluations: dry matter, accumulated nitrogen, crude protein, neutral detergent fiber, acid detergent fiber, holocellulose, cellulose, hemicellulose, lignin, total extracts, and ashes. According to the results of the present study, it is concluded that the application of diazotrophic bacteria alone or in conjunction with nitrogen fertilization, increases productivity and total soluble solids, anticipating maturation, in addition to improving the chemical-bromatological composition of sugarcane plants.

Canopeo and GreenSeeker applications as tools to support tropical pasture management / Aplicativos Canopeo e GreenSeeker como ferramentas de apoio ao manejo de pastagens tropicais

ABSTRACT: This study determined whether Canopeo and GreenSeeker measurements in Megathyrsus maximus can estimate plant height, dry matter mass, morphological components, and content of crude protein and neutral detergent fiber at different days of growth. Five plots of 5 × 25m M. maximus grass were defined: subplots of 1×1m were evaluated every three days, in which the pasture shows 3, 6, 9, 12, 15, 18, 21, 24, 27, and 30 days of regrowth. The subplot was evaluated for canopy height and mass accumulation. The canopeo index (CI) obtained at a higher height was lower than those obtained at a smaller height. Higher measurement height increased the normalized difference vegetation index (NDVI) relative to 0.10 m. The highest indexes were observed since 18 d of regrowth. Except for the CI evaluated at 0.10 m of height, the indexes were not correlated to the chemical composition of the forage. The CI and NDVI were positively correlated to plant height, dry matter mass, and leaf index, whereas both were negatively correlated with stalk index. Thus, lower evaluation heights for CI and NDVI can be a good predictor of forage height. Values of 0.83 and 85.8 for NDVI and CI, respectively, indicated an appropriate time to start the grazing of M. maximus.

RESUMO: O presente estudo teve como objetivo avaliar se os índices obtidos com Canopeo e GreenSeeker (NDVI) em Megathyrsus maximum são capazes de estimar altura de plantas, massa de matéria seca, componentes morfológicos e teor de proteína bruta e fibra em detergente neutro em diferentes dias de crescimento. Cinco parcelas de 5 × 25 m de capim M.maximum foram definidas: sub parcelas de 1 × 1 m foram avaliadas a cada três dias, nas quais a pastagem apresenta 3, 6, 9, 12, 15, 18, 21, 24, 27 e 30 dias de rebrota. A sub parcela foi avaliada quanto à altura do dossel e acúmulo de massa. O índice do Canopeo (IC) obtido em maior altura foi menor do que o obtido em menor altura. A maior altura de medição aumentou o NDVI em relação a 0,10 m. Os maiores índices foram observados desde 18 dias de rebrota. Exceto para o IC avaliado a 0,10 m de altura, os índices não foram correlacionados com a composição química da forragem. IC e NDVI correlacionaram-se positivamente com a altura da planta, massa de MS e índice foliar, e negativamente com o índice de colmos. Assim, menores alturas de avaliação para IC e NDVI podem ser um bom preditor da altura da forragem. Valores de 0,83 e 85,8 para NDVI e IC, respectivamente, indicam um momento adequado para o início do pastejo de Megathyrsus maximum.

Intake and apparent nutrient digestibility, ruminal parameters, and nitrogen balance of lambs fed with corn and forage-free diets with sodium bicarbonate / Consumo e digestibilidade aparente dos nutrientes, parâmetros ruminais e balanço de N de cordeiros confinados recebendo dietas com milho e sem forragem com bicarbonato de sódio

This study evaluated forage-free diets supplemented with sodium bicarbonate (SB) based on ground flint corn on intake, apparent nutrient digestibility, ruminal parameters, and N balance. Four rumen-cannulatedDorper × Santa Inês lambs, with a body weight of 43.4 ± 0.85 kg (mean ± standard deviation) were used. The experimental design was a 4×4 Latin square. Each experimental period lasted 21 days. Experimental diets were: positive control, a diet without SB containing 400 g/kg DM of soybean hulls (CONT), and three diets based on ground flint corn, containing 10 (10SB), 20 (20SB) or 30 (30SB) g/kg DM of SB. Intake of neutral and acid detergent fiber was greater for CONT (P < 0.01) when compared to SB. Intake and apparent nutrient digestibility of dry and organic matter, ether extract, and crude protein did not differ. Intake (P = 0.01) and digestibility (P < 0.01) of non-fiber carbohydrates were greater for SB diets and neutral detergent fiber digestibility was greater (P = 0.03) for CONT. The total digestible nutrients and metabolizable energy were greater for SB (P = 0.04). Rumen concentrations of acetate (P < 0.01), isobutyrate (P < 0.01), butyrate (P < 0.01), isovalerate (P < 0.01), and total short-chain fatty acids (P < 0.01) were greater for CONT. Ruminal pH showed a quadratic (P = 0.05) response to SB levels, with greater values for 20SB. Sodium bicarbonate was efficient in increasing ruminal pH at the level of 20 g/kg DM without interfering with the intake and apparent nutrient digestibility characteristics.

O presente estudo avaliou a suplementação de bicarbonato de sódio (BS) em dietas sem forragem à base de milho moído sobre o consumo e a digestibilidade aparente dos nutrientes, os parâmetros ruminais e o balanço de N. Foram utilizados quatro cordeiros Dorper × Santa Inês, com um peso corporal inicial de 43,4 ± 0,85 kg (média ± desvio padrão). O delineamento experimental foi quadrado latino 4×4. Cada período experimental durou 21 dias. As dietas experimentais foram: controle positivo, sem BS, contendo 400 g/kg de MS de casca de soja (CONT) e três dietas à base de milho moído, sem casca de soja, contendo 10 (10BS), 20 (20BS) ou 30 (30BS) g/kg de MS de BS. O consumo de fibra em detergente neutro e ácido foi maior (P < 0,01)para o CONT quando comparado com BS. O consumo e a digestibilidade aparente da matéria seca, matéria orgânica, extrato etéreo e proteína bruta não diferiram entre os tratamentos. O consumo (P = 0,01) e digestibilidade (P < 0,01) dos carboidratos não fibrosos foram superiores para as dietas contendo BS e a digestibilidade da fibra em detergente neutro foi maior para o CONT. Os teores de nutrientes digestivos totais e energia metabolizável foram superiores para as dietas contendo BS (P = 0,04). A concentração ruminal de acetato (P < 0,01), isobutirato (P < 0,01), butirato (P < 0,01), isovalerato (P < 0,01) e ácidos graxos totais de cadeia curta (P < 0,01) foram mais elevadas para o CONT. O pHruminal apresentou resposta quadrática (P = 0,05) em relação aos níveis de BS, sendo observado maior pH para os animais alimentados com a dieta 20BS. O BS foi eficiente em aumentar o pHruminal no teor de 20 g/kg MS sem interferir no consumo e na digestibilidade aparente dos nutrientes. Portanto, 20 g/kg de MS é a dose indicada de BS em dietas sem forragem para terminação de cordeiros em confinamento.

In situ ruminal degradability of forage cactus-based diets associated with pornunça silage / Degradabilidade ruminal in situ de dietas a base de palma forrageira associada à silagem de pornunça

The knowledge of ruminal degradation kinetics of forage cactus-based diets associated with Euphorbiaceae species of the genus Manihot assist in understanding nutrient quality and use, contributing to the indication of combinations of these ingredients in ruminant diets. This study aimed to evaluate the in situ ruminal degradability of diets with increasing association of forage cactus Elephant Ear (MEE) and pornunça (Manihot sp.) silage, as well as to analyze and evaluate the indigestible neutral detergent fiber (FDNi) content of forage cactus MEE and pornunça silage. The treatments consisted of diets with cactus pear associated with pornunça silage in the roughage portion in the proportions of 375, 250, 125 and 0 g kg-1 of DM. The feed was composed of 50% roughage and 50% concentrate of dry matter. Incubation times of 0, 6, 12, 24, 48, 96, and 144 hours were evaluated for the ingredients and 0, 2, 4, 6, 8, 12, 24, 48, 72, and 96 hours for the diets. Incubation to obtain the iNDF was given for 288 hours. The experimental design consisted of randomized blocks, with three replications. Regarding roughage ingredients, MEE showed higher average disappearance, potential degradability (PD), and effective degradability (ED) in relation to pornunça silage, as well as lower NDFi content. The proportion of 375 g kg-1 MEE and 125 g kg-1 pornunça silage provided higher fractions a, b, and c, increasing ED considering the three passage rates, with higher PD. In conclusion, the association between MEE and pornunça silage at a proportion of 375 g kg-1 MEE and125g kg-1 pornunça silage of the roughage portion increases the in situ degradability of dry matter, crude protein, and neutral detergent fiber.

O conhecimento da cinética de degradação ruminal de dietas compostas por palma-forrageira associadas com espécies de Euphorbiaceae do gênero Manihot auxiliam na compreensão da qualidade e aproveitamento dos nutrientes, com contribuição para a indicação de combinações destes ingredientes em dietas para ruminantes. Objetivou-se avaliar a degradabilidade ruminal in situ de dietas com crescente associação de palma-forrageira Orelha de Elefante Mexicana (OEM) e silagem de pornunça (Manihot sp.), assim como, avaliar o teor de fibra em detergente neutro indigestível (FDNi) da palma OEM e da silagem de pornunça. Os tratamentos consistiram em dietas com palma forrageira associada à silagem de pornunça na porção volumosa nas proporções de 375, 250, 125 e 0 g kg-1 na MS. A dieta foi composta por 50% de volumoso e 50% de concentrado da matéria seca. Foram avaliados os tempos de incubação de 0, 6,12, 24, 48, 96 e 144 para os ingredientes e 0, 2, 4, 6, 8 ,12, 24, 48, 72 e 96 para as dietas. A incubação para a obtenção do FDNi foi realizada por 288 horas. O delineamento experimental utilizado foi em blocos casualizados, com três repetições. Quanto aos ingredientes volumosos, a palma OEM apresentou maior desaparecimento médio, degradabilidade potencial (DP) e degradabilidade efetiva (DE) em relação à silagem de pornunça, bem como menor teor de FDNi. A proporção de 375 g kg-1 OEM e 125 g kg-1 de silagem de pornunça propiciou maiores frações a, b e c, atuando no aumento da DE considerando as três taxas de passagem, com maior DP. Conclui-se que à associação de palma OEM e silagem de pornunça na proporção de 375 g kg-1 OEM e 125 g kg-1 da silagem de pornunça da porção volumosa aumenta a degradabilidade in situ da matéria seca, proteína bruta e fibra em detergente neutro.

Yield, chemical composition, and efficiency of utilization of applied nitrogen from BRS Kurumi pastures / Produção, composição química e eficiência da utilização de nitogênio aplicado de pastagens de BRS Kurumi

ABSTRACT: The objective was to evaluate the effect of nitrogen fertilization levels on productivity, and nutritional value of BRS Kurumi managed at different residue heights during rainy and dry periods of the year. The pasture was managed in three residue heights (20, 35, and 50 cm) and three nitrogen fertilization levels (0, 100, and 200 kg N/ha/year) during the rainy and dry seasons. When the height of the grass of each plot reached 80 cm, the Kurumi was cut manually at the pre-established residue heights. Around 89% of the dry matter (DM) production was observed during the rainy season. The residue height of 35 cm together with doses of 100 or 200 kg N/ha/year, and the residue height of 20 cm, with a dose of 100 kg N/ha/year were the treatments that had the highest DM production during the rainy period. No differences in DM production were observed between tested treatments during the dry period. The Kurumi cultivated during the dry period had the highest content of neutral detergent fiber and lowest digestibility. The Kurumi managed with residue heights of 20 and 35 cm and fertilized with 100 kg N/ha had the highest values of efficiency of utilization applied nitrogen. The use of 20 or 35 cm of residue height and 100 kg of N/ha/year is recommended to obtain greater biomass production and nutrients, nutritional quality, and nitrogen use efficiency.

RESUMO: O objetivo foi avaliar o efeito dos níveis de fertilização nitrogenada na produtividade e no valor nutritivo do BRS Kurumi quando manejado em diferentes alturas de resíduos durante os períodos seco e chuvoso do ano. O capim foi manejado em três alturas de resíduos (20, 35 e 50 cm) em associação com três níveis de fertilização com nitrogênio (0, 100 e 200 kg N/ha/ ano), durante o período seco e chuvoso do ano. Quando a altura do capim de cada parcela atingiu 80 cm, o Kurumi foi cortado nas alturas de resíduos pré-estabelecidas. Cerca de 89% da produção de MS foi observada durante o período chuvoso. A altura do resíduo de 35 cm juntamente com doses de 100 ou 200 kg N/ha/ ano, e a altura do resíduo de 20 cm, com dose de 100 kg N/ha/ ano foram os tratamentos que apresentaram maior produção de MS durante o período chuvoso. Não foram observadas diferenças na produção de MS entre os tratamentos testados durante o período da seca. O Kurumi cultivado no período da seca apresentou maior teor de fibra em detergente neutro e menor digestibilidade. O Kurumi manejado com alturas de resíduo de 20 e 35 cm e fertilizado com 100 kg N/ha apresentou os maiores valores de eficiência de utilização do nitrogênio aplicado. Recomenda-se a utilização de 20 ou 35 cm de altura do resíduo e 100 kg de N/ha/ ano para obter maior produção de biomassa e nutrientes, qualidade nutricional e eficiência no uso do nitrogênio.

Intake, digestibility, and ingestive behavior of sheep fed with thornless Mandacaru, cactus pear genotypes Orelha de Elefante Mexicana and Miúda

This study aimed to evaluate the intake, digestibility, and ingestive behavior of sheep fed with different species of forage cacti. Fifteen sheep (17.27kg ± 1 kg) were distributed in a completely randomized experimental design with three treatments and five replicates. The treatments were diets on a dry matter basis composed of 430.9 g kg-1of thornless Mandacaru cactus (Cereus hildmannianus), 525.7 g kg-1of cactus pear cv. Orelha de Elefante Mexicana (Opuntia stricta) and 492.1 g kg-1of cactus pear cv. Miúda (Nopalea cochenillifera) in addition to Sabiá hay (Mimosa caesalpiniaefolia) (194.7 to 233.8 g kg-1), plus concentrate feed. The intake of the dry matter,organic matter, ether extract, neutral detergent fiber, total carbohydrates, non-fiber carbohydrates, total digestible nutrientsand voluntary water intake in g day-1was not differ (p > 0.05) by experimental diets. There were no differences (p > 0.05) in digestibility coefficients of the dry matter,organic matter, neutral detergent fiber, total carbohydrates, non-fiber carbohydrates, and total digestible nutrientsbetween the experimental diets. The feeding times differed (p < 0.05) between diets, with a higher value for the Opuntiadiet, while the rumination times, feeding efficiency, and rumination efficiency did not differ (p > 0.05). The cactus Cereusand Opuntiaand Nopaleahave similar nutritional value in sheep's diet.(AU)

Agromorphological, yield and quality characteristics of two populations of alfalfa developed by mass selection / Características agromorfológicas, produtivas e de qualidade de duas populações de alfafa desenvolvidas por seleção massal

This study was designed to test the two alfalfa advanced populations (L-1737 and L-1738) with four control cultivars (Bilensoy-80, Gözlü, Savas and Plato) for agromorphological, yield and quality properties under irrigated conditions in two various locations in Ankara, Turkey in the years of 2013, 2014, and 2015. The experiments were arranged in a randomized block design with four replications. Results of the study showed that there were statistically significant differences in plant height and stem diameter as well as stem number among the study materials. Statistically significant differences were observed for green forage yield (P < 0.05), while not in dry matter yield among the study materials. The average green forage and dry matter yields were reported as 67.0 t ha-1 and 13. 0 t ha-1, respectively. Green forage and dry matter yields in the advanced populations of L-1737 and L-1738 were 69.2 and 67.4 t ha-1; 13.7 and 13.6 t ha-1, respectively. Those yield values were 6.5 and 3.7%; 4.3 and 3.4% higher in green forage and dry matter yields of the advanced populations of L-1737 and L-1738 compared to the control cultivar Bilensoy-80, respectively. These increases in green forage and dry matter yields were due to the effect of the mass selection method. Statistically significant differences were observed for acid detergent fiber, neutral detergent fiber, and relative feeding value among the study materials, except crude protein contents. It was concluded that the two advanced populations had good adaptation ability, high yield and good quality performance under irrigated conditions and could be used as commercial cultivars.

Este estudo foi desenhado para testar duas populações avançadas de alfafa (L-1737 e L-1738) com quatro cultivares controle (Bilensoy-80, Gözlü, Savas e Plato) para propriedades agromorfológicas, produtividade e qualidade sob condições irrigadas em dois locais diferentes em Ancara, Turquia, nos anos de 2013, 2014 e 2015. Os experimentos foram dispostos em delineamento de blocos casualizados com quatro repetições. Os resultados do estudo mostraram que houveram diferenças estatisticamente significativas na altura das plantas e diâmetro do colmo, bem como no número de colmos entre os materiais avaliados. Foram observadas diferenças estatisticamente significativas para a produtividade de forragem verde (P < 0.05), enquanto não foram observadas diferenças na produção de matéria seca entre os materiais estudados. As produtividades médias de forragem verde e matéria seca foram de 67.0 t ha-1 e 13.0 t ha-1, respectivamente. Os rendimentos de forragem verde e matéria seca nas populações avançadas de L-1737 e L-1738 foram 69.2 e 67.4 t ha-1; 13.7 e 13.6 t ha-1, respectivamente. Esses valores de rendimento foram 6.5 e 3.7%; 4.3 e 3.4% maiores em rendimentos de forragem verde e matéria seca das populações avançadas de L-1737 e L-1738 em relação à cultivar controle Bilensoy-80, respectivamente. Esses aumentos nos rendimentos de forragem verde e matéria seca foram devidos ao efeito do método de seleção massal. Diferenças, estatisticamente significativas, foram observadas para fibra em detergente ácido, fibra em detergente neutro e valor alimentar relativo entre os materiais estudados, exceto nos teores de proteína bruta. Concluiu-se que as duas populações avançadas apresentaram boa capacidade de adaptação, alta produtividade e bom desempenho em condições irrigadas, podendo ser utilizadas como cultivares comerciais.

Biomass sorghum silages with sugarcane / Silagem de sorgo biomassa com cana-de-açúcar / Ensilaje de sorgo biomasa con caña de azúcar

The storage of forage to be offered at different times of the year are viable alternatives for all production systems, and sorghum biomass has been highlighted for this purpose. As preservedforage, it was hypothesized that sugarcane can contribute to the fermentation process. Theobjective was to evaluate the inclusion of different levels of sugarcane (0, 20, 40 and60%) inthe silage of three biomass sorghum genotypes (B012, B017 and B018). The material wasensiled using PVC silos and after 60 days the silos were opened and the contents of drymatter, mineral matter, organic matter, crude protein, neutral detergent fiber, acid detergent fiber, hemicellulose, lignin, and hydrogen potential were determined. The experiment wasconducted in a completely randomized design, in a factorial scheme with four replications. The data were analyzed through the analysis of variance followed by multiple comparisonbyTukey's test (α < 0.05) and linear regression. The biomass sorghum genotypes respondedsatisfactorily to the fermentation process, resulting in quality silages. However, the inclusionof sugarcane did not improve the quality of the silages, and its inclusion in the silage of thegenotypes evaluated is not recommended.(AU)

O armazenamento de forragem a ser ofertada em diferentes épocas do ano são alternativasviáveis para todos os sistemas de produção, e a biomassa de sorgo tem se destacado paraestefim. Como forragem preservada, levantou-se a hipótese de que a cana-de-açúcar podecontribuir para o processo de fermentação. O objetivo foi avaliar a inclusão de diferentesníveis de cana-de-açúcar (0, 20, 40 e 60%) na silagem de três genótipos de sorgo biomassa(B012, B017 e B018). O material foi ensilado em silos de PVC e após 60 dias foramabertosedeterminados os teores de matéria seca, matéria mineral, matéria orgânica, proteína bruta, fibra em detergente neutro, fibra em detergente ácido, hemicelulose, lignina e potencial dehidrogênio. O experimento foi conduzido em delineamento inteiramente casualizado, emesquema fatorial com quatro repetições. Os dados foram analisados por meio da análisedevariância seguida de comparação múltipla pelo teste de Tukey (α < 0,05) e regressãolinear. Os genótipos de sorgo biomassa responderam satisfatoriamente ao processo de fermentação, resultando em silagens de qualidade. No entanto, a inclusão da cana-de-açúcar não melhoroua qualidade das silagens, não sendo recomendada sua inclusão na silagemdos genótipos avaliados.(AU)

Los acopios de forrajes para ser ofrecidos en diferentes épocas del año son alternativas viablespara todos los sistemas de producción, y para ello se ha destacado la biomasa de sorgo. Comoforraje conservado, se planteó la hipótesis de que la caña de azúcar puede contribuir al proceso de fermentación. El objetivo fue evaluar la inclusión de diferentes niveles de cañadeazúcar (0, 20, 40 y 60%) en el ensilaje de tres genotipos de sorgo de biomasa (B012, B017yB018). El material se ensiló en silos de PVC y después de 60 días se abrieron los silos ysedeterminaron los contenidos de materia seca, materia mineral, materia orgánica, proteínacruda, fibra detergente neutro, fibra detergente ácido, hemicelulosa, lignina y potencial dehidrógeno. El experimento se condujo en un diseño completamente al azar, en un esquemafactorial con cuatro repeticiones. Los datos fueron analizados a través del análisis de varianzaseguido de comparación múltiple por la prueba de Tukey (α < 0,05) y regresión lineal. Losgenotipos de sorgo de biomasa respondieron satisfactoriamente al proceso de fermentación, dando como resultado ensilajes de calidad. Sin embargo, la inclusión de caña de azúcar nomejoró la calidad de los ensilajes, por lo que no se recomienda su inclusión en los ensilajesdelos genotipos evaluados.(AU)

Chemical and physical variability of traditional varieties of corn (Zea mays L) grown in the Juruá Valley, Acre, Brazil / Variabilidade nutricional e física de variedades tradicionais de milho (Zea mays L) cultivados no vale do Juruá, Acre, Brasil

The traditional varieties of maize grown by family farmers are a source of genetic variability and are essential for food security. Thus, the present study aims to evaluate, based on physical and chemical components, the behavior of the genetic variability of traditional maize cultivated on non-flooded and floodplain areas, in the Juruá region, state of Acre. The characteristics consisted of the determination of dry matter, mineral material, crude protein, ether extract, gross energy, neutral detergent fiber, starch and in vitro digestibility of dry matter, vitreousness and grain density. Data were analyzed using descriptive statistics, associated with multivariate analysis of principal components (PCA), with the aid of the R software. By means of the PCA, the varieties grown on non-flooded and floodplain areas formed 3 distinct groups, in which the vitreosity in the grains ranged from 73.7% to 79.46%, so that the reddish grain varieties had a greater presence of endosperm vitreous and higher density, with a strong and positive correlation between these variables. The yellow and orange-yellow grain varieties showed greater adherence to energy, starch and greater digestibility. Therefore, the traditional varieties cultivated on non-flooded and floodplain areas, present variations in relation to the physical and chemical analyses.

As variedades tradicionais de milho cultivados pelos agricultores familiares constituem-se em fonte de variabilidade genética e são fundamentais para segurança alimentar. Assim, o presente trabalho tem como objetivo, avaliar, com base em componentes físicos e químicos, o comportamento da variabilidade genética de milho tradicional cultivadas em terra firme e praia, na regional Vale do Juruá, estado do Acre. As características constaram da determinação de matéria seca, material mineral, proteína bruta, extrato etéreo, energia bruta, fibra em detergente neutro, amido e digestibilidade in vitro da matéria seca, vitreosidade e densidade dos grãos. Os dados foram analisados mediante estatística descritiva, associado à análise multivariada de componentes principais (PCA), com auxílio do software R. Por meio da PCA, as variedades cultivadas em terra firme e praia formaram 3 grupos distintos, na qual a vitreosidade nos grãos variou de 73,7% a 79,46%, de modo que, as variedades de grãos avermelhadas apresentaram maior presença de endosperma vítreo e maior densidade, havendo uma correlação forte e positiva entre essas variáveis. Já as variedades de grãos amarelas e amarelas-oranges apresentaram maior aderência a energia, amido e maior digestibilidade. Portanto, as variedades tradicionais cultivadas em terra firme e praia, apresentam variações em relação as análises físicas e químicas.

Nutritional, physiological, hematological, and biochemical responses of lambs fed increasing levels of Pornunça silage

Pornunça (Manihot sp.) is a potential forage to feed ruminants in drylands worldwide; however, evaluations of animal diets are necessary. This study assessed intake and digestibility of dry matter and nutrients, physiological responses, ingestive behavior, water intake, as well as hematological and biochemical responses of lambs fed diets containing increasing levels of Pornunça silage (PS) replacing Tifton-85 bermudagrass hay (Cynodon spp.). Treatments consisted of 0, 33, 66, and 100 % PS considering the roughage portion of the diet (% dry matter - DM). The experimental design was completely randomized with six replicates. Cyanide acid (HCN) levels in fresh Pornunça leaves were 207.7 mg kg-¹ DM and 76.78 mg kg-¹ DM in PS. Intake of DM and crude protein (CP), and neutral detergent fiber corrected for ash and protein (NDFap) increased linearly with increasing levels of PS in the diet. Digestibility of DM, NDF, and CP were higher with increasing levels of PS. Idle time decreased linearly, while water balance, water intake via food, and total water in feces increased with PS. Nitrogen balance, physiological, and hematological responses were not influenced by treatments, while the final alanine aminotransferase increased for lambs fed all diets evaluated. The PS up to 100 % of the roughage portion increased DM and nutrient intake and digestibility without altering physiological responses and the hematological and biochemical parameters, resulting in greater water intake via food.

Yield, chemical composition, and efficiency of utilization of applied nitrogen from BRS Kurumi pastures / Produção, composição química e eficiência da utilização de nitogênio aplicado de pastagens de BRS Kurumi

The objective was to evaluate the effect of nitrogen fertilization levels on productivity, and nutritional value of BRS Kurumi managed at different residue heights during rainy and dry periods of the year. The pasture was managed in three residue heights (20, 35, and 50 cm) and three nitrogen fertilization levels (0, 100, and 200 kg N/ha/year) during the rainy and dry seasons. When the height of the grass of each plot reached 80 cm, the Kurumi was cut manually at the pre-established residue heights. Around 89% of the dry matter (DM) production was observed during the rainy season. The residue height of 35 cm together with doses of 100 or 200 kg N/ha/year, and the residue height of 20 cm, with a dose of 100 kg N/ha/year were the treatments that had the highest DM production during the rainy period. No differences in DM production were observed between tested treatments during the dry period. The Kurumi cultivated during the dry period had the highest content of neutral detergent fiber and lowest digestibility. The Kurumi managed with residue heights of 20 and 35 cm and fertilized with 100 kg N/ha had the highest values of efficiency of utilization applied nitrogen. The use of 20 or 35 cm of residue height and 100 kg of N/ha/year is recommended to obtain greater biomass production and nutrients, nutritional quality, and nitrogen use efficiency.

O objetivo foi avaliar o efeito dos níveis de fertilização nitrogenada na produtividade e no valor nutritivo do BRS Kurumi quando manejado em diferentes alturas de resíduos durante os períodos seco e chuvoso do ano. O capim foi manejado em três alturas de resíduos (20, 35 e 50 cm) em associação com três níveis de fertilização com nitrogênio (0, 100 e 200 kg N/ha/ ano), durante o período seco e chuvoso do ano. Quando a altura do capim de cada parcela atingiu 80 cm, o Kurumi foi cortado nas alturas de resíduos pré-estabelecidas. Cerca de 89% da produção de MS foi observada durante o período chuvoso. A altura do resíduo de 35 cm juntamente com doses de 100 ou 200 kg N/ ha/ ano, e a altura do resíduo de 20 cm, com dose de 100 kg N/ha/ ano foram os tratamentos que apresentaram maior produção de MS durante o período chuvoso. Não foram observadas diferenças na produção de MS entre os tratamentos testados durante o período da seca. O Kurumi cultivado no período da seca apresentou maior teor de fibra em detergente neutro e menor digestibilidade. O Kurumi manejado com alturas de resíduo de 20 e 35 cm e fertilizado com 100 kg N/ha apresentou os maiores valores de eficiência de utilização do nitrogênio aplicado. Recomenda-se a utilização de 20 ou 35 cm de altura do resíduo e 100 kg de N/ha/ ano para obter maior produção de biomassa e nutrientes, qualidade nutricional e eficiência no uso do nitrogênio.

Canopeo and GreenSeeker applications as tools to support tropical pasture management / Aplicativos Canopeo e GreenSeeker como ferramentas de apoio ao manejo de pastagens tropicais

This study determined whether Canopeo and GreenSeeker measurements in Megathyrsus maximus can estimate plant height, dry matter mass, morphological components, and content of crude protein and neutral detergent fiber at different days of growth. Five plots of 5 × 25m M. maximus grass were defined: subplots of 1×1m were evaluated every three days, in which the pasture shows 3, 6, 9, 12, 15, 18, 21, 24, 27, and 30 days of regrowth. The subplot was evaluated for canopy height and mass accumulation. The canopeo index (CI) obtained at a higher height was lower than those obtained at a smaller height. Higher measurement height increased the normalized difference vegetation index (NDVI) relative to 0.10 m. The highest indexes were observed since 18 d of regrowth. Except for the CI evaluated at 0.10 m of height, the indexes were not correlated to the chemical composition of the forage. The CI and NDVI were positively correlated to plant height, dry matter mass, and leaf index, whereas both were negatively correlated with stalk index. Thus, lower evaluation heights for CI and NDVI can be a good predictor of forage height. Values of 0.83 and 85.8 for NDVI and CI, respectively, indicated an appropriate time to start the grazing of M. maximus.

O presente estudo teve como objetivo avaliar se os índices obtidos com Canopeo e GreenSeeker (NDVI) em Megathyrsus maximum são capazes de estimar altura de plantas, massa de matéria seca, componentes morfológicos e teor de proteína bruta e fibra em detergente neutro em diferentes dias de crescimento. Cinco parcelas de 5 × 25 m de capim M.maximum foram definidas: sub parcelas de 1 × 1 m foram avaliadas a cada três dias, nas quais a pastagem apresenta 3, 6, 9, 12, 15, 18, 21, 24, 27 e 30 dias de rebrota. A sub parcela foi avaliada quanto à altura do dossel e acúmulo de massa. O índice do Canopeo (IC) obtido em maior altura foi menor do que o obtido em menor altura. A maior altura de medição aumentou o NDVI em relação a 0,10 m. Os maiores índices foram observados desde 18 dias de rebrota. Exceto para o IC avaliado a 0,10 m de altura, os índices não foram correlacionados com a composição química da forragem. IC e NDVI correlacionaram-se positivamente com a altura da planta, massa de MS e índice foliar, e negativamente com o índice de colmos. Assim, menores alturas de avaliação para IC e NDVI podem ser um bom preditor da altura da forragem. Valores de 0,83 e 85,8 para NDVI e IC, respectivamente, indicam um momento adequado para o início do pastejo de Megathyrsus maximum.

Does the use of corn and soybean hulls affect calf performance in the preweaning period?

The objective of this study was to assess the effects of two levels of soybean hulls (0 and 400.1 g/kg) and whole or ground corn in the diet of newborn crossbred dairy male calves on intake, performance, blood indicators, and feeding cost. Twenty-eight calves with an average weight of 33.0±6.2 kg and four days of age were distributed into four treatment groups in a completely randomized design (n = 7) for 56 days. Weekly samples of feed, diets, and leftovers were collected to determine dry matter and nutrient intakes. To evaluate apparent digestibility, samples were taken using titanium dioxide as a maker. Blood samples were also collected to evaluate blood indicators. The inclusion of soybean hulls resulted in greater neutral detergent fiber intake by the calves, but reduced their non-fibrous carbohydrates intake, which was also reduced by the use of whole corn in the diet. Although the total digestible nutrients content of diets decreased with the use of whole corn and inclusion of soybean hulls, its intake did not vary (0.75 kg/d), regardless of the factors analyzed. The apparent digestibilities of dry matter (0.87 kg/kg) and crude protein (0.89 kg/kg) were similar, resulting in similar performance between the animals, regardless of the factors analyzed. In the quantities evaluated, the use of soybean hulls or whole corn did not affect blood indicators and was insufficient to reduce feed costs; the cost of daily feed was $2.06, while the cost per kilogram of gain was $3.74. The inclusion of up to 400.1 g/kg of soybean hulls or the replacement of ground corn with whole corn does not affect the performance of crossbred dairy calves during the preweaning period, demonstrating that both can be used in animal feed during this phase of production.(AU)

Identical thermal stress coupled with different temperature and humidity combinations affects nutrient digestibility and gut metabolites of laying hens

The present study investigated whether the same temperature-humidity index (THI) values under different conditions of air temperature and relative humidity (RH) would affect the thermoregulatory, nutritional, and behavioral responses of laying hens. One hundred twenty Hy-Line Brown laying hens (60-weeks-old) were divided equally in two environmental chambers: 26 °C with 70% RH (hRH75) and 30 °C with 30% RH (hT75) for 28 days. The two ambient environments (hRH75 and hT75) had an identical THI value of 75, calculated using an empirical formula for laying hens. Neither hRH75 nor hT75 affected rectal and body-surface temperatures and heart and respiratory rates. The concentration of volatile fatty acids in fecal excreta were altered by the thermal treatments. hT75 vs. hRH75 decreased the proportion of acetate and increased the proportion of propionate in fecal samples. hT75 vs. hRH75 lowered the digestibility of dry matter, crude protein, and neutral detergent fiber at 14 days. Thermal treatments did not affect heat stress-associated behavioral responses including feeding, drinking, panting, and wing elevation at any stage. Laying hens exposed to the same THI at different temperatures and RH exhibit equal physiological responses including rectal and body-surface temperatures, heart and respiratory rates, and behavioral responses. Nonetheless, high-temperature treatment (hT75; 30 °C and 30% RH) vs. low temperature treatment (hRH75; 26 °C and 70% RH) affects nutrient digestibility and gut metabolites, suggesting that there are negligible but discernable responses to temperature in the gut physiology.(AU)

Effect of replacing corn with cactus pear on the performance and carcass traits and meat quality of feedlot finished lambs / Efeito da substituição do milho pela palma forrageira sobre o desempenho, características de carcaça e qualidade da carne de cordeiros terminados em confinamento

The aim was to evaluate the effect of replacing ground corn with spineless cactus from two species Orelha de Elefante Mexicana-OEM (Opuntia stricta Haw.) and Gigante (Opuntia ficus-indica Mill) in a total mixed ration for finishing lambs evaluating its effects on intake, ingestive behavior, performance, carcass traits, commercial cuts and physiochemical composition of the meat. Eighteen crossbred lambs with average body weight of 15.0 ± 2.32 kg were distributed in a completely randomized design with three treatments (OEM and Gigante spineless cactus and ground corn as control treatment) and six replications. Spineless cactus species replacing ground corn in lambs diets does not change the intake of dry matter (DMI), crude protein and neutral detergent fiber or time (min/d) of ingestion, rumination and idleness, or final body weight gain. Lambs fed with ground corn and "Gigante" cactus presented a higher total weigh gain (TWG) and average daily weight gain (ADG) when compared to OEM spineless specie. Ground corn diet promoted better hot and cold carcass yield in lambs receiving spineless cactus, regardless of species. Feed and total costs (kg/lamb) were higher for the ground corn diet. The replacement of ground corn with spineless cactus did not change moisture, protein, and ash meat contents, as well as water holding capacity, cooking loss, shear force, and a* color intensity. However, there was an effect for the meat lipid content, b* and L* color intensity and color index, where the species of cactus Gigante on ground corn presented the highest lipid content and yellowness (b*) intensity and lower L* color compared to OEM. Spineless cactus species Gigante can replace ground corn as a source of energy in diets for finishing lambs because it significantly improves the financial income for the producer without changing the ADG, DMI, ingestive behavior and yield of commercial cuts.

Objetivou-se avaliar o efeito da substituição do milho moído por duas espécies de palma forrageira, Orelha de Elefante Mexicana-OEM (Opuntia stricta Haw.) e espécie Gigante (Opuntia ficus-indica Mill) em uma ração completa para terminação de cordeiros avaliando seus efeitos sobre consumo, comportamento ingestivo, desempenho, características de carcaça, cortes comerciais e composição físico-química da carne. Foram utilizados dezoito cordeiros sem padrão racial definido com peso corporal médio de 15,0 ± 2,32 kg e foram distribuídos em delineamento inteiramente casualizado com três tratamentos (OEM e palma Gigante e grão de milho como controle) e seis repetições. As duas espécies de palma forrageira em substituição ao milho moído na dieta de cordeiros não modificaram o consumo de matéria seca (CMS) proteína bruta e fibra em detergente neutro nem o tempo (min/dia) de ingestão, ruminação e ócio ou ganho corporal final. Cordeiros alimentados com milho moído e palma Gigante apresentaram maior ganho de peso total (GPT) e médio diário (GMD) em relação à espécie OEM. A dieta com milho moído promoveu melhor rendimento de carcaça quente e fria comparando cordeiros recebendo palma forrageira, independente da espécie. Os custos com ração e total (kg/cordeiro) foram maiores para a dieta com milho moído. A substituição do milho moído pela palma forrageira não alterou os teores de umidade, proteína e cinzas da carne, bem como a capacidade de retenção de água, perda por cozimento, força de cisalhamento e intensidade de cor a*. No entanto, houve efeito para o teor de lipídios da carne, índice de intensidade de cor b* e L*, sendo que a espécie de palma Gigante e o milho grão moído apresentou o maior teor de lipídios e intensidade de amarelo (b*) e menor cor L* em relação ao OEM. A espécie palma forrageira Gigante pode substituir o milho moído como fonte de energia em dietas para cordeiros em terminação, pois melhora significativamente o rendimento financeiro do produtor sem alterar o GMD, CMS, comportamento ingestivo e rendimento dos cortes comerciais.

Evaluation of extruded roughage with different additives in sheep diet / Evaluation of extruded roughage with different additives in sheep diet

The purpose was to evaluate the effect of extruded roughage Foragge® with different additives on intake, digestibility nutrients and nitrogen balance of sheep. Twenty adults, non-pregnant ewes with average weight 68 kg were used. The treatments were extruded roughage with additives (essential oil, virginiamycin, unpurified inactive yeast, tannin and purified inactive yeast). The design was in randomized blocks. The means were contrasted by SNK test, and the fecal score was tested by the Kruskal Wallis test (1952), at 5% significance. There was no difference in the intake of dry matter, crude protein, nitrogen, water, water in relation to dry matter, neutral detergent fiber (NDF), and acid detergent fiber (p > 0.05). As well as the digestibility of dry matter, crude protein and neutral detergent fiber, the fecal weight, fecal nitrogen, retained nitrogen and nitrogen retained in relation to nitrogen ingested (p > 0.05). However, dry matter intake as a function of body weight and metabolic weight, urinary nitrogen, hemicellulose intake, and hemicellulose as a function of NDF, were higher in the Foragge Factor® treatment (p < 0.05). The inclusion of different additives in the extruded roughage improved nutritional parameters, without causing disturbances.  

The purpose was to evaluate the effect of extruded roughage Foragge® with different additives on intake, digestibility nutrients and nitrogen balance of sheep. Twenty adults, non-pregnant ewes with average weight 68 kg were used. The treatments were extruded roughage with additives (essential oil, virginiamycin, unpurified inactive yeast, tannin and purified inactive yeast). The design was in randomized blocks. The means were contrasted by SNK test, and the fecal score was tested by the Kruskal Wallis test (1952), at 5% significance. There was no difference in the intake of dry matter, crude protein, nitrogen, water, water in relation to dry matter, neutral detergent fiber (NDF), and acid detergent fiber (p > 0.05). As well as the digestibility of dry matter, crude protein and neutral detergent fiber, the fecal weight, fecal nitrogen, retained nitrogen and nitrogen retained in relation to nitrogen ingested (p > 0.05). However, dry matter intake as a function of body weight and metabolic weight, urinary nitrogen, hemicellulose intake, and hemicellulose as a function of NDF, were higher in the Foragge Factor® treatment (p < 0.05). The inclusion of different additives in the extruded roughage improved nutritional parameters, without causing disturbances.  

Chemical composition of forage watermelon fruit at different maturity stage or storage length / Chemical composition of forage watermelon fruit at different maturity stage or storage length

This study aimed to assess the chemical responses of forage watermelon fruit at different maturity stages or storage lengths, performing two experimental tests. In the first test, four maturity stages were assessed: 30, 45, 60, and 75 days after anthesis, with four replicates. In the second test, fruits were maintained under three storage lengths: T1D (harvest day), T3M (3 months after harvest), and T6M (6 months after harvest), with eight replicates. Experimental design was completely randomized in both experimental tests. Fruit maturity stage did not affect crude protein, total carbohydrate, neutral detergent fiber, in vitro dry matter digestibility (IVDMD), pulp firmness, soluble solids content and total pectin content, but increased acid detergent fiber content from 45 days after anthesis. Storage length up to six months after harvest increased ash, crude protein and IVDMD, and reduced the content of soluble solids. Forage watermelon fruit can be harvested from 30 to 75 days after anthesis equivalent to 75 - 120 days after planting, and they can be stored under tree shade up to 6 months after harvest.

This study aimed to assess the chemical responses of forage watermelon fruit at different maturity stages or storage lengths, performing two experimental tests. In the first test, four maturity stages were assessed: 30, 45, 60, and 75 days after anthesis, with four replicates. In the second test, fruits were maintained under three storage lengths: T1D (harvest day), T3M (3 months after harvest), and T6M (6 months after harvest), with eight replicates. Experimental design was completely randomized in both experimental tests. Fruit maturity stage did not affect crude protein, total carbohydrate, neutral detergent fiber, in vitro dry matter digestibility (IVDMD), pulp firmness, soluble solids content and total pectin content, but increased acid detergent fiber content from 45 days after anthesis. Storage length up to six months after harvest increased ash, crude protein and IVDMD, and reduced the content of soluble solids. Forage watermelon fruit can be harvested from 30 to 75 days after anthesis equivalent to 75 - 120 days after planting, and they can be stored under tree shade up to 6 months after harvest.