Salicylic acid concentrations and forms of application mitigate water stress in sour passion fruit seedlings / Concentrações e formas de aplicação de ácido salicílico mitiga o estresse hídrico em mudas de maracujazeiro-azedo

The present study aimed to evaluate concentrations and forms of application of salicylic acid used for water stress mitigation on the gas exchange and growth of yellow passion fruit. The experimental design was arranged in randomized blocks in a 4 × 4 × 2 factorial scheme, with four concentrations of salicylic acid (SA) via foliar application (0.0, 0.7, 1.4, and 2.1 mM), four SA concentrations via fertigation (0.0, 0.7, 1.4, and 2.1 mM), and two irrigation depths estimated based on the actual evapotranspiration - ETr (50 and 100% of ETr), with three replications. Water stress negatively affected the physiology and growth of yellow passion fruit seedlings at 75 days after sowing (DAS). The application of salicylic acid, regardless of the form of application, attenuates the effects of water stress on gas exchange and growth of yellow passion fruit, with the best results obtained when applying a concentration of 1.30 mM via leaf or 0.90 mM via fertirrigation. The combination of foliar application of AS and fertigation contributed to improve photosynthetic and growth parameters under water conditions of 50 and 100% of ETr. The foliar application of AS presents superior responses to the application via fertigation. These results reinforce the hypothesis that the attenuation of water stress by salicylic acid is related to the maintenance of gas exchange, which depends on the concentration and form of application, and studies testing combinations throughout the crop cycle become promising for advances in knowledge from the action of this phytohormone on abiotic stress.

O presente estudo teve como objetivo avaliar as concentrações e formas de aplicação do ácido salicílico como mitigador do estresse hídrico sobre as trocas gasosas e parâmetros de crescimento do maracujazeiro amarelo. O delineamento experimental foi em blocos casualizados, em esquema fatorial 4 × 4 × 2, com tratamentos constituídos por quatro concentrações de ácido salicílico (SA) via aplicação foliar (0,0, 0,7, 1,4 e 2,1 mM), quatro concentrações de SA via fertirrigação (0,0, 0,7, 1,4 e 2,1 mM) e duas lâminas de irrigação estimadas com base na evapotranspiração real - ETr (50 e 100% da ETr), com três repetições. O estresse hídrico afetou negativamente a fisiologia e o crescimento das mudas de maracujazeiro amarelo aos 75 dias após o semeio (DAS). A aplicação de ácido salicílico, independente da forma de aplicação, atenua os efeitos do estresse hídrico nas trocas gasosas e crescimento do maracujazeiro amarelo, com os melhores resultados obtidos ao se aplicar a concentração de 1,30 mM via foliar ou 0,90 mM via fertirrigação. A combinação de aplicação de AS via foliar e fertirrigação contribuiu para melhorar os parâmetros fotossintéticos e de crescimento nas condições hídricas de 50 e 100% da ETr. A aplicação via foliar de AS apresenta respostas superiores a aplicação via fertirrigação. Esses resultados reforçam a hipótese de que a atenuação do estresse hídrico pelo ácido salicílico está relacionada a manutenção das trocas gasosas, a qual depende da concentração e forma de aplicada, sendo que estudos testando combinações ao longo do ciclo da cultura se torna promissores para avanços nos conhecimentos provenientes da atuação desse fitormônio sobre o estresse abiótico.

Potassium fertilization as salt stress attenuator in sour passion fruit / Adubação potássica como atenuante do estresse salino em maracujazeiro-azedo

Water scarcity associated with irregular rainfall in the semi-arid region of Northeastern Brazil stands out as a limiting factor for agricultural production. Thus, the use of waters with high concentration of salts is an alternative to expand irrigated agriculture in this region. In this context, this study evaluated the water status, intercellular electrolyte leakage, photosynthetic pigments, and gas exchange of 'BRS SC1' sour passion fruit as a function of irrigation with water of different levels of salinity and potassium doses. The experiment was carried out in pots adapted as drainage lysimeters under field conditions at the Experimental Farm of the Federal University of Campina Grande in São Domingos - PB, Brazil. The experimental design was randomized blocks, in a 5 × 4 factorial scheme, whose treatments were obtained by combining two factors: five levels of electrical conductivity of irrigation water - ECw (0.3, 1.1, 1.9, 2.7, and 3.5 dS m-1), associated with four potassium doses (60, 80, 100, and 120% of the recommendation), with three replicates. Water with electrical conductivity greater than 0.3 dS m-1 reduced the relative water content, chlorophyll a and chlorophyll b contents, CO2 assimilation rate, and instantaneous water use efficiency of 'BRS SC1' sour passion fruit plants. The estimated potassium dose of 85% of the recommendation (equivalent to 293 g per plant per year) mitigated the deleterious effects of salt stress on stomatal conductance, transpiration, internal CO2 concentration, and instantaneous carboxylation efficiency of passion fruit 'BRS SC1'.

Water scarcity associated with irregular rainfall in the semi-arid region of Northeastern Brazil stands out as a limiting factor for agricultural production. Thus, the use of waters with high concentration of salts is an alternative to expand irrigated agriculture in this region. In this context, this study evaluated the water status, intercellular electrolyte leakage, photosynthetic pigments, and gas exchange of 'BRS SC1' sour passion fruit as a function of irrigation with water of different levels of salinity and potassium doses. The experiment was carried out in pots adapted as drainage lysimeters under field conditions at the Experimental Farm of the Federal University of Campina Grande in São Domingos - PB, Brazil. The experimental design was randomized blocks, in a 5 × 4 factorial scheme, whose treatments were obtained by combining two factors: five levels of electrical conductivity of irrigation water - ECw (0.3, 1.1, 1.9, 2.7, and 3.5 dS m-1), associated with four potassium doses (60, 80, 100, and 120% of the recommendation), with three replicates. Water with electrical conductivity greater than 0.3 dS m-1 reduced the relative water content, chlorophyll a and chlorophyll b contents, CO2 assimilation rate, and instantaneous water use efficiency of 'BRS SC1' sour passion fruit plants. The estimated potassium dose of 85% of the recommendation (equivalent to 293 g per plant per year) mitigated the deleterious effects of salt stress on stomatal conductance, transpiration, internal CO2 concentration, and instantaneous carboxylation efficiency of passion fruit 'BRS SC1'.

Row-Col and Bayesian approach seeking to improve the predictive capacity and selection of passion fruit

Methods for genetic improvement of semi-perennial species, such as passion fruit, often involve large areas, unbalanced data, and lack of observations. Some strategies can be applied to solve these problems. In this work, different models and approaches were tested to improve the precision of estimates of genetic evaluation models for several characteristics of the passion fruit. A randomized block design (RBD) model was compared to a posteriori correction, adding two factors to the model (post-hoc blocking Row-Col). These models were also combined with the frequentist and Bayesian approaches to identify which combination yields the most accurate results. These approaches are part of a strategic plan in a perennial plant breeding program to select promising genitors of passion to compose the next selection cycle. For Bayesian, we tested two priors, defining different values for the distribution parameters of effect variances of the model. We also performed a cross-validation test to choose a priori values and compare the frequentist and Bayesian approaches using the root mean square error (RMSE) and the correlation between the predicted and observed values, called Predictive capacity of the model (PC). The model with the post-hoc blocking Row-Col design captured the spatial variability for productivity and number of fruits, directly affecting the experimental precision. Both approaches applied to the models showed a similar performance, with predictive capacity and selective efficiency leading to the selection of the same individuals.

Quantum yield, chlorophyll, and cell damage in yellow passion fruit under irrigation strategies with brackish water and potassium / Rendimento quântico, clorofila e dano celular em maracujazeiro-amarelo sob estratégias de irrigação com água salobra e potássio

The occurrence of water sources with high concentrations of salts in the semiarid region of Northeast Brazil stands out as a limiting factor in the cultivation of irrigated yellow passion fruit. Thus the search for irrigation strategies with brackish water is fundamental for the sustainability of irrigated crops. The objective of the present study was to determine the quantum yield, chlorophyll levels, and cell damage in yellow passion fruit cultivated under different irrigation strategies with brackish water and potassium fertilization. The experiment was conducted under field conditions in São Domingos - PB, Brazil, adopting a randomized block design in a 6×2 factorial scheme, consisting of six strategies of use of brackish water applied in the different phenological stages of crop and two doses of potassium (60 and 100% of the recommendation), with four replicates. Two levels of irrigation water salinity were used, with low (1.3 dS m-1) and high electrical conductivity (4.0 dS m-1). The potassium dose of 100% recommendation corresponds to the application of 345 g of K2O per plant per year. The use of brackish water (4.0 dS m-1) increased chlorophyll fluorescence and negatively affected the photosynthetic pigments of yellow passion fruit, regardless of the phenological stage. Salt stress intensified intercellular electrolyte leakage in passion fruit plants under higher dose of potassium. Our results show that fertilization equivalent to 100% of the potassium recommendation is excessive for yellow passion fruit 'BRS GA1' in the tropical semiarid region under salt stress conditions.

A ocorrência de mananciais com altas concentrações de sais no semiárido nordestino destaca-se como fator limitante no cultivo do maracujazeiro-amarelo irrigado. Assim, a busca por estratégias de irrigação com água salobra é fundamental para a sustentabilidade da agricultura irrigada. O objetivo deste trabalho foi determinar o rendimento quântico, os teores de clorofila e dano celular em maracujazeiro-amarelo cultivado sob diferentes estratégias de irrigação com água salobra e adubação potássica. O experimento foi conduzido em condições de campo em São Domingos - PB, Brasil, adotando-se o delineamento em blocos casualizados em esquema fatorial 6×2, correspondendo a seis estratégias de uso de água salobra aplicadas nos diferentes estádios fenológicos da cultura e duas doses de potássio (60 e 100% da recomendação), com quatro repetições. Foram utilizados dois níveis de salinidade da água de irrigação, com baixa (1,3 dS m-1) e alta condutividade elétrica (4,0 dS m-1). A dose de potássio de 100% corresponde à aplicação de 345 g de K2O por planta por ano. O uso de água salobra (4,0 dS m-1) aumentou a fluorescência da clorofila e afetou negativamente os pigmentos fotossintéticos do maracujá-amarelo, independente do estádio fenológico. O estresse salino intensificou o extravasamento de eletrólitos em plantas de maracujazeiro adubadas sob alta dose de potássio. Os resultados mostram que a adubação equivalente a 100% da recomendação de potássio é excessiva para o cultivo do maracujazeiro-amarelo 'BRS GA1' no semiárido tropical, sob condições de estresse salino.

Micorrização e respiração microbiana do solo sob o cultivo de maracujá e pinhão manso em diferentes regimes hídricos / Mycorrization and microbial respiration in soil under passion fruit and jatropha in different water regimes

Os fungos micorrízicos arbusculares (FMAs) e a população microbiana do solo ocupam papel de destaque em amenizar os efeitos causados pelo estresse hídrico em diversas culturas, entretanto se faz necessário estabelecer parâmetros sobre a dinâmica da interação desses microrganismos com as plantas em condições de estresse. Diante do exposto, o trabalho teve por objetivo avaliar a micorrização ea respiração microbiana do solo cultivado com mudas de maracujá amarelo e pinhão manso submetidas a diferentes níveis de água. O experimento foi realizado em vasos na casa de vegetação localizada na Universidade Estadual de Goiás, unidade de Ipameri. O delineamento utilizado foi inteiramente casualizado, e analisado em um esquema fatorial 2 x 7 sendo duas espécies de plantas (maracujá e pinhão manso) e sete níveis de fornecimento de água (25%, 50%, 75%, 100%, 125%, 150% e 175%) em relação a capacidade de campo (CC) do solo com cinco repetições, totalizando 70 parcelas experimentais de cultivo. Foram avaliadas a respiração microbiana do solo (RM), número de esporos micorrízicos (ESP) e colonização micorrízica (CM). Em termos de RM os solos responderam de forma distinta a alteração da umidade do solo, onde a microbiota do solo com pinhão manso se mostrou mais adaptada até aos 175% da CC que do maracujá. A condição de excesso hídrico provocou uma diminuição no ESP do solo de ambas a culturas, porém com efeito sendo mais significativo na cultura do maracujá que apresentou uma média de 77,2 esporos 50g-1 de solo. A CM do pinhão manso e do maracujazeiro foram afetadas negativamente com o aumento dos níveis de umidade do solo, porém o pinhão manso apresentou em média uma taxa de CM 38,5% maior que o maracujá. De maneira geral a microbiota do solo e os FMAs sob mudas de pinhão manso responderam melhor ao estresse hídrico.(AU)

Arbuscular mycorrhizal fungi (AMF) and the soil microbial population occupy a prominent role in mitigating the effects caused by water stress in several crops. However, it is necessary to establish parameters on the dynamics of the interaction of these microorganisms with plants under stress conditions. Given the above, this study aimed to evaluate the mycorrhization and microbial respiration of soil cultivated with yellow passion fruit and jatropha seedlings subjected to different water levels. The experiment was carried out in pots in a greenhouse located at the State University of Goiás, Ipameri unit. The design used was completely randomized and analyzed in a 2x 7 factorial scheme with two plant species (passion fruit and Jatropha curcas) and seven levels of water supply (25%, 50%, 75%, 100%, 125%, 150% and,175%) in relation to the field capacity (FC) of the soil with five replications, totaling 70 experimental cultivation plots. In addition, soil microbial respiration (MR), number of mycorrhizal spores (SPO) and mycorrhizal colonization (MC) were also evaluated. In terms of MR, the soils responded differently to changes in soil moisture, where the microbiota of the soil with Jatropha curcaswas more adapted up to 175% of CC than that of passion fruit. The excess water condition caused a decrease in the SPOof the soil of both crops, but with an effect being more significant in the passion fruit crop, which presented an average of 77.2 spores 50g-1of soil. The MC of Jatropha curcasand passion fruit were negatively affected with the increase of soil moisture levels, however,the Jatropha curcaspresented an average rate of MC 38.5% higher than the passion fruit. In general, the soil microbiota and the AMF under Jatropha seedlings responded better to water stress.(AU)

Elephant grass silage with addition of regional by-products

This study examines the effect of adding coffee husks (CH), cacao by-product (CBP) and passion fruit by-product (PBP) (fresh-matter basis) in the silage of elephant grass cv. Napier on nutritional characteristics. The experiment was laid out in a completely randomized design with a 3 × 4 factorial arrangement represented by three additives (CH, CBP or PBP) and four inclusion levels (0, 12, 24 or 36%). Four replicates were used per treatment. The material was ensiled in experimental mini-silos that were opened 60 days later for chemical analysis of the produced silages. The dry matter content of the silages with CH and PBP included at levels greater than 25% was adequate. The silage with PBP inclusion showed the highest crude protein levels and the best results for in vitro dry matter digestibility. In the silage containing CH, the neutral detergent fiber content decreased linearly with increasing inclusion of the husks. The silage with CBP showed the lowest dry matter and crude protein levels and the highest pH. In conclusion, the inclusion of up to 36% PBP in the ensiling of elephant grass is recommended, as the resulting material has potential for use in ruminant feeding at times of forage scarcity.(AU)

Physicochemical and sensorial characterization of yacon mixed juice with bioactive properties / Caracterização físico-química e sensorial de suco misto de yacon com propriedades bioativas

This study developed a palatable, acceptable, and functional mixed juice of pineapple (Ananas comosus var. Comosus), passion fruit (Passiflora edulis flavicarpa degener), and yacon (Smallanthus sonchifolius), with enhanced bioactive and prebiotic compounds. The ideal proportion of fruit and yacon was derived after planning a mixture with six formulations and by evaluating their bioactive compounds and organic acid profile. The best formulation sensory evaluation was performed by the Just-About-Right test (JAR) and global acceptance. The chosen mixed juice (M6 - 30% pineapple, 45% passion fruit, and 30% yacon) presented results that showed elevated levels of antioxidant activity (82.5% DPPH* inhibition), carotenoids (0.97 mg.100g-¹), and total polyphenols (70.28 mg.100g-¹). Total flavonols (23.95 mg.100g-¹), total anthocyanins (1.37 mg.100g-¹), gallic acid (3.13 µg.mL-¹), quercetin (2.25 µg.mL-¹), syringic acid (1.01 µg.mL-¹), citric acid (949.30 mg.100g-¹), malic acid (140.35 mg.100g-¹), and 0.58% of dietary fiber were also reported. Sensorially, consumers perceived high acidity, strong flavor, and a fibrous texture, which might have affected the global acceptance of the juice (6.0). Results indicated the feasibility of obtaining a functional and palatable mixed juice using yacon.

Este estudo objetivou desenvolver suco misto funcional, palatável e aceitável de abacaxi (Ananas comosus var. Comosus), maracujá (Passiflora edulis flavicarpa degenerer) e yacon (Smallanthus sonchifolius), enriquecido com compostos bioativos e prebióticos. A proporção ideal entre as frutas e o yacon foi resultado de planejamento de mistura com seis formulações e avaliação de perfil de compostos bioativos e ácidos orgânicos. A melhor formulação foi avaliada sensorialmente pelo teste Just About Right (JAR) e por aceitação global. O suco misto escolhido (M6 - 30% de abacaxi, 45% de maracujá e 30% de yacon) apresentou altos valores para atividade antioxidante (82,5% de inibição de DPPH *), carotenoides (0,97 mg/100g) polifenóis totais (70,28 mg/100g). Também foram detectados flavonóis totais (23,95 mg/100g), antocianinas totais (1,37 mg/100g), ácido gálico (3,13 µg/mL), quercetina (2,25 µg/mL), ácido siríngico (1,01 µg/mL), ácido cítrico (949,30 mg/100g), ácido málico (140,35 mg/100g) e 0,58% de fibra alimentar. Sensorialmente, os consumidores perceberam alta acidez, sabor forte e textura fibrosa, o que pode ter afetado a aceitação global (6,0). Os resultados indicam a possibilidade de obtenção de um suco misto funcional e palatável com yacon.

Gas exchange, growth, and quality of passion fruit seedlings cultivated with saline water / Trocas gasosas, crescimento e qualidade de mudas de maracujazeiro cultivadas com águas salinas

This study was conducted to evaluate the gas exchange, growth, and quality of passion fruit cultivars under irrigation with waters of different salinity levels. The experiment was conducted in a greenhouse, in Pombal-PB, Brazil, using a randomized block design in a 5 ? 2 factorial arrangement, with five levels of electrical conductivity of irrigation water (0.3, 1.1, 1.9, 2.7, and 3.5 dS m-1) and two passion fruit cultivars (BRS Sol do Cerrado and Guinezinho), with three plants per plot and four replicates. The reduction in transpiration, intercellular CO2 concentration and CO2 assimilation rate in passion fruit plants grown with saline water was related to factors of stomatal and non-stomatal origin. Gas exchange and growth of passion fruit cultivars were negatively affected by water salinity from 0.3 dS m-1 at 75 days after sowing. Despite the reduction in the growth of the passion fruit cultivars, irrigation with water of up to 3.5 dS m-1 could be used to obtain seedlings with acceptable quality. Based on the relative yield of total dry phytomass, the passion fruit cultivars BRS Sol do Cerrado and Guinezinho were classified as moderately sensitive to water salinity during the seedling phase.(AU)

Desenvolveu-se este trabalho com o objetivo de avaliar as trocas gasosas, o crescimento e a qualidade de cultivares de maracujazeiro sob irrigação com águas de diferentes níveis salinos. O experimento foi conduzido em casa de vegetação, em Pombal-PB, utilizando-se o delineamento de blocos casualizados em arranjo fatorial 5 × 2, sendo cinco níveis de condutividade elétrica da água de irrigação (0,3; 1,1; 1,9; 2,7 e 3,5 dS m-1) e duas cultivares de maracujazeiro (BRS Sol do Cerrado e Guinezinho), com três plantas por parcela e quatro repetições. A redução na transpiração, concentração intercelular de CO2 e taxa de assimilação de CO2 nas plantas de maracujazeiro cultivadas com águas salinas está relacionado a fatores de origem estomáticos e não estomáticos. As trocas gasosas e o crescimento das cultivares de maracujazeiro foram afetados de forma negativa pela salinidade da água a partir de 0,3 dS m-1, aos 75 dias após o semeio. Apesar da redução no crescimento nas cultivares de maracujazeiro, a irrigação com água de até 3,5 dS m-1 pode ser utilizada para obtenção de mudas com qualidade aceitável. Com base no rendimento relativo de fitomassa seca total, as cultivares de maracujazeiro BRS Sol do Cerrado e Guinezinho foram classificados como moderadamente sensível a salinidade da água na fase de formação de mudas.(AU)

Gas exchange, growth, and quality of passion fruit seedlings cultivated with saline water / Trocas gasosas, crescimento e qualidade de mudas de maracujazeiro cultivadas com águas salinas

This study was conducted to evaluate the gas exchange, growth, and quality of passion fruit cultivars under irrigation with waters of different salinity levels. The experiment was conducted in a greenhouse, in Pombal-PB, Brazil, using a randomized block design in a 5 ? 2 factorial arrangement, with five levels of electrical conductivity of irrigation water (0.3, 1.1, 1.9, 2.7, and 3.5 dS m-1) and two passion fruit cultivars (BRS Sol do Cerrado and Guinezinho), with three plants per plot and four replicates. The reduction in transpiration, intercellular CO2 concentration and CO2 assimilation rate in passion fruit plants grown with saline water was related to factors of stomatal and non-stomatal origin. Gas exchange and growth of passion fruit cultivars were negatively affected by water salinity from 0.3 dS m-1 at 75 days after sowing. Despite the reduction in the growth of the passion fruit cultivars, irrigation with water of up to 3.5 dS m-1 could be used to obtain seedlings with acceptable quality. Based on the relative yield of total dry phytomass, the passion fruit cultivars BRS Sol do Cerrado and Guinezinho were classified as moderately sensitive to water salinity during the seedling phase.

Desenvolveu-se este trabalho com o objetivo de avaliar as trocas gasosas, o crescimento e a qualidade de cultivares de maracujazeiro sob irrigação com águas de diferentes níveis salinos. O experimento foi conduzido em casa de vegetação, em Pombal-PB, utilizando-se o delineamento de blocos casualizados em arranjo fatorial 5 × 2, sendo cinco níveis de condutividade elétrica da água de irrigação (0,3; 1,1; 1,9; 2,7 e 3,5 dS m-1) e duas cultivares de maracujazeiro (BRS Sol do Cerrado e Guinezinho), com três plantas por parcela e quatro repetições. A redução na transpiração, concentração intercelular de CO2 e taxa de assimilação de CO2 nas plantas de maracujazeiro cultivadas com águas salinas está relacionado a fatores de origem estomáticos e não estomáticos. As trocas gasosas e o crescimento das cultivares de maracujazeiro foram afetados de forma negativa pela salinidade da água a partir de 0,3 dS m-1, aos 75 dias após o semeio. Apesar da redução no crescimento nas cultivares de maracujazeiro, a irrigação com água de até 3,5 dS m-1 pode ser utilizada para obtenção de mudas com qualidade aceitável. Com base no rendimento relativo de fitomassa seca total, as cultivares de maracujazeiro BRS Sol do Cerrado e Guinezinho foram classificados como moderadamente sensível a salinidade da água na fase de formação de mudas.

Elevated plus-maze and the hole board test for use as a quality control assay of herbal medicines based on passiflora incarnata linnaeus / Teste do labirinto em cruz elevado e campo vazado para o uso como ensaios de controle de qualidade de medicamentos fitoterápicos a base de passiflora incarnata linnaeus

In the search for the reduction of the side effects caused by allopathy, phytotherapeutic products are already on the veterinary market. This study aimed was to evaluate Elevated Plus-Maze and Hole Board test as an analytical methodology for quality control of anxiolytic medicines based on Passiflora incarnata. The efficacy of three brands commercial differents of Passiflora incarnata extracts was tested in the Elevated Plus-maze (for anxiolytic effect) and the Hole Board test (for locomotor and neophilia activity) in Wistar rats. Treatment with the P. incarnata brands revealed a significant 3-fold increase in the length of stay in the open arms in the LCE. In the ambulation, animals from bands 1 and 3 presented a significant increase, being brand 2 the only one that did not present this relevance. In the neophilia, Passiflora brand 1 presented a significant increase of 3 times and groups 2 and 3 an increase of approximately 2 times. The results showed that different brands of Passiflora have a satisfactory quality standard related to biological activity and showed that the use of Elevated plus-maze/Hole board test can be an effective tool in the quality control of herbal medicines with anxiolytic activity associated with the phytochemical analysis.

Na busca pela redução dos efeitos colaterais causados pela alopatia, os fitoterápicos já se encontram disponíveis no mercado veterinário. O objetivo deste estudo foi avaliar os testes neurocomportamentais Labirinto em Cruz Elevado e Campo Vazado como metodologias analíticas para o controle de qualidade de medicamentos ansiolíticos a base de Passiflora incarnata. A eficácia de três diferentes marcas comerciais de extrato de Passiflora incarnata foi testada no Labirinto em Cruz Elevado (para efeitos ansiolíticos) e no Campo Vazado (para atividade locomotora e busca por novidade) em ratos Wistar. O tratamento com as marcas de P. incarnata demonstrou aumento significativo de 3 vezes no tempo de permanência nos braços abertos no Labirinto em Cruz Elevado. Na deambulação, animais das marcas 1 e 3 apresentaram aumento significativo, sendo a marca 2 a única que não apresentou relevância para este parâmetro. Na neofilia, a marca 1 de Passiflora apresentou aumento significativo de 3 vezes e as marcas 2 e 3 aumento de aproximadamente 2 vezes. Os resultados mostraram que diferentes marcas de Passiflora apresentam um padrão de qualidade satisfatório relacionado à atividade biológica e mostraram que a utilização dos testes Labirinto em Cruz Elevado e Campo Vazado podem ser ferramentas eficazes no controle de qualidade de fitoterápicos com atividade ansiolítica associadas à análises fitoquímicas.

Elevated plus-maze and the hole board test for use as a quality control assay of herbal medicines based on passiflora incarnata linnaeus / Teste do labirinto em cruz elevado e campo vazado para o uso como ensaios de controle de qualidade de medicamentos fitoterápicos a base de passiflora incarnata linnaeus

In the search for the reduction of the side effects caused by allopathy, phytotherapeutic products are already on the veterinary market. This study aimed was to evaluate Elevated Plus-Maze and Hole Board test as an analytical methodology for quality control of anxiolytic medicines based on Passiflora incarnata. The efficacy of three brands commercial differents of Passiflora incarnata extracts was tested in the Elevated Plus-maze (for anxiolytic effect) and the Hole Board test (for locomotor and neophilia activity) in Wistar rats. Treatment with the P. incarnata brands revealed a significant 3-fold increase in the length of stay in the open arms in the LCE. In the ambulation, animals from bands 1 and 3 presented a significant increase, being brand 2 the only one that did not present this relevance. In the neophilia, Passiflora brand 1 presented a significant increase of 3 times and groups 2 and 3 an increase of approximately 2 times. The results showed that different brands of Passiflora have a satisfactory quality standard related to biological activity and showed that the use of Elevated plus-maze/Hole board test can be an effective tool in the quality control of herbal medicines with anxiolytic activity associated with the phytochemical analysis.(AU)

Na busca pela redução dos efeitos colaterais causados pela alopatia, os fitoterápicos já se encontram disponíveis no mercado veterinário. O objetivo deste estudo foi avaliar os testes neurocomportamentais Labirinto em Cruz Elevado e Campo Vazado como metodologias analíticas para o controle de qualidade de medicamentos ansiolíticos a base de Passiflora incarnata. A eficácia de três diferentes marcas comerciais de extrato de Passiflora incarnata foi testada no Labirinto em Cruz Elevado (para efeitos ansiolíticos) e no Campo Vazado (para atividade locomotora e busca por novidade) em ratos Wistar. O tratamento com as marcas de P. incarnata demonstrou aumento significativo de 3 vezes no tempo de permanência nos braços abertos no Labirinto em Cruz Elevado. Na deambulação, animais das marcas 1 e 3 apresentaram aumento significativo, sendo a marca 2 a única que não apresentou relevância para este parâmetro. Na neofilia, a marca 1 de Passiflora apresentou aumento significativo de 3 vezes e as marcas 2 e 3 aumento de aproximadamente 2 vezes. Os resultados mostraram que diferentes marcas de Passiflora apresentam um padrão de qualidade satisfatório relacionado à atividade biológica e mostraram que a utilização dos testes Labirinto em Cruz Elevado e Campo Vazado podem ser ferramentas eficazes no controle de qualidade de fitoterápicos com atividade ansiolítica associadas à análises fitoquímicas.(AU)

Nutrient suppression in passion fruit species: an approach to leaf development and morphology / Supressão de nutrientes em espécies de maracujazeiro: uma abordagem no desenvolvimento e na morfologia foliar

The production of passion fruit in commercial areas requires a good correction of nutrients in the soil, however, with nutritional restrictions it can cause symptoms of deficiency expressed mainly in the leaves and stems, which may appear spots, reduced growth, changes in the architecture of these organs mainly in the its size and shape. This work aimed to know the effects caused by the suppression of nutrients in passion fruit species with an approach on their development and leaf morphology. The experimental design was completely randomized (DIC) in a 2x6 factorial scheme where the first factor was composed of two passion fruit species: P. gibertiiand P. edulis, interacting with the availability of nutrients, that is, a control group with the supply of all nutrients, with nitrogen suppression (N); phosphorus (P); potassium (K); magnesium (Mg) and iron (Fe), totaling 12 treatments with four replications totaling 48 plots or plastic vessels per experiment. The species P. edulisshowed better responses to the variables of development andleaf morphology. The species P. gibertiiis more susceptible to nutrient deficiency and showed changes in the color and shape of its leaves with the restriction of nutrients. The nutrients Fe and Mg caused greater restrictions on the development and leaf morphology of passion fruit species. It is necessary to carry out a new study with the species P. gibertiiregarding the appropriate concentrations of N, P and K provided in planting and cover, as this species is used as a rootstock in commercial areas.(AU)

A produção de maracujá em áreas comerciais demanda de uma boa correção de nutrientes no solo, no entanto, com restrições nutricionais pode provocar sintomas de deficiência expressada principalmente nas folhas e caule, podendo aparecer manchas, redução no crescimento, mudança na arquitetura desses órgãos principalmente no seu tamanho e formato. Esse trabalho teve por objetivo conhecer os efeitos provocados pela supressão de nutrientes em espécies de maracujazeiros com uma abordagem no seu desenvolvimento e na morfologia foliar. O delineamento experimental foiinteiramente casualizado (DIC) em esquema fatorial 2x6 onde o primeiro fator foi composto por duas espécies de maracujazeiros sendo elas: P. gibertiie P. edulis, interagindo com a disponibilidade de nutrientes, ou seja, um grupo controle com o fornecimento de todos os nutrientes, com as supressões de nitrogênio (N); fósforo (P); potássio (K); magnésio (Mg) e ferro (Fe), totalizando 12 tratamentos com quatro repetições totalizando 48parcelas ou vasos plásticos por experimento. A espécie P. edulisapresentou melhores respostas para as variáveis de desenvolvimento e morfologia foliar. A espécie P. gibertiié mais susceptível a deficiência de nutrientes e apresentou mudanças na coloração e formato das suas folhas com a restrição de nutrientes. Os nutrientes Fe e Mg causaram maiores restrições no desenvolvimento e na morfologia foliar das espécies de maracujá. É necessário realizar novo estudo com a espécie P. gibertiiquanto às concentrações adequadas de N, P e K fornecidas no plantio e cobertura, pois essa espécie é utilizada como porta enxerto em áreas comerciais.(AU)

Nutrient suppression in passion fruit species: an approach to leaf development and morphology / Supressão de nutrientes em espécies de maracujazeiro: uma abordagem no desenvolvimento e na morfologia foliar

The production of passion fruit in commercial areas requires a good correction of nutrients in the soil, however, with nutritional restrictions it can cause symptoms of deficiency expressed mainly in the leaves and stems, which may appear spots, reduced growth, changes in the architecture of these organs mainly in the its size and shape. This work aimed to know the effects caused by the suppression of nutrients in passion fruit species with an approach on their development and leaf morphology. The experimental design was completely randomized (DIC) in a 2x6 factorial scheme where the first factor was composed of two passion fruit species: P. gibertiiand P. edulis, interacting with the availability of nutrients, that is, a control group with the supply of all nutrients, with nitrogen suppression (N); phosphorus (P); potassium (K); magnesium (Mg) and iron (Fe), totaling 12 treatments with four replications totaling 48 plots or plastic vessels per experiment. The species P. edulisshowed better responses to the variables of development andleaf morphology. The species P. gibertiiis more susceptible to nutrient deficiency and showed changes in the color and shape of its leaves with the restriction of nutrients. The nutrients Fe and Mg caused greater restrictions on the development and leaf morphology of passion fruit species. It is necessary to carry out a new study with the species P. gibertiiregarding the appropriate concentrations of N, P and K provided in planting and cover, as this species is used as a rootstock in commercial areas.

A produção de maracujá em áreas comerciais demanda de uma boa correção de nutrientes no solo, no entanto, com restrições nutricionais pode provocar sintomas de deficiência expressada principalmente nas folhas e caule, podendo aparecer manchas, redução no crescimento, mudança na arquitetura desses órgãos principalmente no seu tamanho e formato. Esse trabalho teve por objetivo conhecer os efeitos provocados pela supressão de nutrientes em espécies de maracujazeiros com uma abordagem no seu desenvolvimento e na morfologia foliar. O delineamento experimental foiinteiramente casualizado (DIC) em esquema fatorial 2x6 onde o primeiro fator foi composto por duas espécies de maracujazeiros sendo elas: P. gibertiie P. edulis, interagindo com a disponibilidade de nutrientes, ou seja, um grupo controle com o fornecimento de todos os nutrientes, com as supressões de nitrogênio (N); fósforo (P); potássio (K); magnésio (Mg) e ferro (Fe), totalizando 12 tratamentos com quatro repetições totalizando 48parcelas ou vasos plásticos por experimento. A espécie P. edulisapresentou melhores respostas para as variáveis de desenvolvimento e morfologia foliar. A espécie P. gibertiié mais susceptível a deficiência de nutrientes e apresentou mudanças na coloração e formato das suas folhas com a restrição de nutrientes. Os nutrientes Fe e Mg causaram maiores restrições no desenvolvimento e na morfologia foliar das espécies de maracujá. É necessário realizar novo estudo com a espécie P. gibertiiquanto às concentrações adequadas de N, P e K fornecidas no plantio e cobertura, pois essa espécie é utilizada como porta enxerto em áreas comerciais.

Foliar application of H2O2 as salt stress attenuator in 'BRS Rubi do Cerrado' sour passion fruit / Aplicação foliar de H2O2 como atenuante do estresse salino em maracujazeiro-azedo 'BRS Rubi do Cerrado'

Irrigation with saline water causes a reduction in yield, especially in semi-arid regions. Cultivation strategies have been developed to mitigate salt stress on plants, such as the use of hydrogen peroxide. The objective of this study was to evaluate the attenuating effect of hydrogen peroxide on the gas exchange and growth of 'BRS Rubi do Cerrado' sour passion fruit cultivated under irrigation with saline water. The design was completely randomized in split-plot plots, with water salinity levels ECw (0.6, 1.2, 1.8, 2.4, and 3.0 dS m-1) considered the plots and the concentrations of hydrogen peroxide H2O2 (0, 15, 30, and 45 µM) considered the subplots, with three replicates. Gas exchange (stomatal conductance, transpiration, CO2 assimilation rate, intercellular CO2 concentration, instantaneous water use efficiency, and instantaneous carboxylation efficiency), and absolute and relative growth rates in stem diameter were evaluated. An increase in irrigation water salinity from 0.6 dS m-1 reduced gas exchange, and exogenous application of hydrogen peroxide did not promote a significant effect on gas exchange. However, foliar application of hydrogen peroxide at 15 µM increased the growth of 'BRS Rubi do Cerrado' sour passion fruit.(AU)

A irrigação com águas salinas causa redução na produtividade, principalmente nas regiões semiáridas. Estratégias de cultivo vêm sendo desenvolvidas, a exemplo uso de peróxido de hidrogênio para mitigar o estresse salino sobre as plantas. Objetivou-se avaliar o efeito atenuante do peróxido de hidrogênio sobre as trocas gasosas e o crescimento do maracujazeiro-azedo 'BRS Rubi do Cerrado' cultivado sob irrigação com águas salinas. O delineamento foi inteiramente casualizados em parcelas subdivididas, sendo os níveis de salinidade da água - CEa (0,6, 1,2, 1,8, 2,4 e 3,0 dS m-1) consideradas as parcelas, e as concentrações de peróxido de hidrogênio ­ H2O2 (0, 15, 30 e 45 µM) as subparcelas, com três repetições. Foram avaliadas as trocas gasosas (condutância estomática, transpiração, taxa de assimilação de CO2, concentração intercelular de CO2, eficiência instantânea no uso da água e eficiência instantânea de carboxilação), e a taxa de crescimento absoluto e relativo do caule. O aumento na salinidade da água de irrigação a partir da salinidade de 0,6 dS m-1 reduziu as trocas gasosas; a aplicação exógena de peróxido de hidrogênio não promoveu efeito significativos sob as trocas gasosas. A aplicação foliar de 15 µM de peróxido de hidrogênio aumentou o crescimento do maracujazeiro-azedo 'BRS Rubi do Cerrado'.(AU)

Cost reduction and quality enhancement in passion fruit seedlings using alternative substrate / Redução de custo e aumento da qualidade de mudas de maracujá azedo usando substrato alternativo

Obtaining vigorous, quality seedlings is a complex task because it involves multiple physical, chemical and biological factors, with the substrate being a fundamental of this process. Therefore, this study proposes to evaluate different formulations of substrate mixtures and identify which provides better emergence vigor and shoot and root growth in passion fruit (Passiflora edulis Sims). The experiment was laid out in a completely randomized design consisting of 23 substrates with different proportions of Vivatto® and Osmocote®, washed sand, soil, fine coal, coarse coal, enriched hydrogel and enriched vermiculite, with and without the addition of NPK, which were distributed into four replications of four plants per plot. To enrich the hydrogel and vermiculite, foliar fertilizer was used. Emergence vigor was evaluated throughout the seedling emergence period and seedling growth parameters were observed at 60 days after sowing. The data were subjected to multivariate analysis and the groups formed were subjected to analysis of variance. Based on principal component analysis (PCA), four groups were identified. The group composed only of soil + coarse coal and enriched hydrogel (2:2:1) was considered the best treatment, as it favored most of the vegetative growth and emergence vigor traits. Soil plus sand with the addition of NPK was not recommended to grow passion fruit seedlings in any of the proportions used, as there was no emergence.(AU)

A obtenção de mudas com qualidade e vigor é complexa, pois envolve múltiplos fatores físicos, químicos e biológicos sendo o substrato fundamental neste processo, assim, o objetivo deste trabalho foi avaliar diferentes formulações de substrato e identificar qual proporciona melhor vigor de emergência e crescimento de parte aérea e raízes de maracujá amarelo (Passiflora edulis Sims). O delineamento experimental foi inteiramente casualizado, composto por 23 substratos, em diferentes misturas de Vivatto®, Osmocote®, areia lavada, solo, resíduo de carvão fino, carvão grosso, hidrogel enriquecido e vermiculita enriquecida com adubo foliar, além de tratamentos com e sem adição de NPK, distribuídos em quatro repetições de quatro plantas por parcela. O vigor de emergência foi avaliado durante o período de emergência das plântulas e os parâmetros de crescimento das plântulas foram observados aos 60 dias após a semeadura. Os dados foram submetidos à análise multivariada e os grupos formados foram submetidos à análise de variância. Com base na análise de componentes principais (PCA), quatro grupos foram identificados. O grupo composto apenas de solo + carvão vegetal e hidrogel enriquecido (2:2:1), foi considerado o melhor tratamento, pois favoreceu a maioria das características de crescimento vegetativo e vigor de emergência. O solo mais a areia com a adição de NPK não foi recomendado para a produção de mudas de maracujá azedo em nenhuma das proporções utilizadas, pois não houve emergência.(AU)

Cost reduction and quality enhancement in passion fruit seedlings using alternative substrate / Redução de custo e aumento da qualidade de mudas de maracujá azedo usando substrato alternativo

Obtaining vigorous, quality seedlings is a complex task because it involves multiple physical, chemical and biological factors, with the substrate being a fundamental of this process. Therefore, this study proposes to evaluate different formulations of substrate mixtures and identify which provides better emergence vigor and shoot and root growth in passion fruit (Passiflora edulis Sims). The experiment was laid out in a completely randomized design consisting of 23 substrates with different proportions of Vivatto® and Osmocote®, washed sand, soil, fine coal, coarse coal, enriched hydrogel and enriched vermiculite, with and without the addition of NPK, which were distributed into four replications of four plants per plot. To enrich the hydrogel and vermiculite, foliar fertilizer was used. Emergence vigor was evaluated throughout the seedling emergence period and seedling growth parameters were observed at 60 days after sowing. The data were subjected to multivariate analysis and the groups formed were subjected to analysis of variance. Based on principal component analysis (PCA), four groups were identified. The group composed only of soil + coarse coal and enriched hydrogel (2:2:1) was considered the best treatment, as it favored most of the vegetative growth and emergence vigor traits. Soil plus sand with the addition of NPK was not recommended to grow passion fruit seedlings in any of the proportions used, as there was no emergence.

A obtenção de mudas com qualidade e vigor é complexa, pois envolve múltiplos fatores físicos, químicos e biológicos sendo o substrato fundamental neste processo, assim, o objetivo deste trabalho foi avaliar diferentes formulações de substrato e identificar qual proporciona melhor vigor de emergência e crescimento de parte aérea e raízes de maracujá amarelo (Passiflora edulis Sims). O delineamento experimental foi inteiramente casualizado, composto por 23 substratos, em diferentes misturas de Vivatto®, Osmocote®, areia lavada, solo, resíduo de carvão fino, carvão grosso, hidrogel enriquecido e vermiculita enriquecida com adubo foliar, além de tratamentos com e sem adição de NPK, distribuídos em quatro repetições de quatro plantas por parcela. O vigor de emergência foi avaliado durante o período de emergência das plântulas e os parâmetros de crescimento das plântulas foram observados aos 60 dias após a semeadura. Os dados foram submetidos à análise multivariada e os grupos formados foram submetidos à análise de variância. Com base na análise de componentes principais (PCA), quatro grupos foram identificados. O grupo composto apenas de solo + carvão vegetal e hidrogel enriquecido (2:2:1), foi considerado o melhor tratamento, pois favoreceu a maioria das características de crescimento vegetativo e vigor de emergência. O solo mais a areia com a adição de NPK não foi recomendado para a produção de mudas de maracujá azedo em nenhuma das proporções utilizadas, pois não houve emergência.

Digestibility of diets with passion fruit by-product estimated through external and internal markers in dairy heifers

This study examined the influence of the external markers chromium oxide (CO), titanium dioxide (TD), isolated, purified and enriched lignin (LIPE®) and isolated, purified, enriched lignin in nanoparticles (NANOLIPE®) as well as the internal markers indigestible dry matter (iDM), indigestible neutral detergent fiber (iNDF) and indigestible acid detergent fiber (iADF) in diets with inclusion of passion fruit by-product for dairy heifers on the estimation of fecal output and nutrient digestibility. Sixteen Holstein × Zebu crossbred heifers at an average live weight of 363 ± 28 kg were randomly distributed in a completely randomized design where they received diets in which Tifton 85 (Cynodon sp.) hay was replaced with passion fruit by-product (0, 12, 24 and 36%, as-fed basis). The CO, LIPE®, NANOLIPE® and iNDF markers did not differ from the total collection method (p > 0.05) in the estimation of fecal output and nutrient digestibility. The TD and iDM markers overestimated, while iADF underestimated fecal output. Under the presented conditions, we recommend using the CO, LIPE®, NANOLIPE® and iNDF markers to estimate fecal output and nutrient digestibility in diets with inclusion of passion fruit by-product for dairy heifers.(AU)

Digestibility of diets with passion fruit by-product estimated through external and internal markers in dairy heifers

This study examined the influence of the external markers chromium oxide (CO), titanium dioxide (TD), isolated, purified and enriched lignin (LIPE®) and isolated, purified, enriched lignin in nanoparticles (NANOLIPE®) as well as the internal markers indigestible dry matter (iDM), indigestible neutral detergent fiber (iNDF) and indigestible acid detergent fiber (iADF) in diets with inclusion of passion fruit by-product for dairy heifers on the estimation of fecal output and nutrient digestibility. Sixteen Holstein × Zebu crossbred heifers at an average live weight of 363 ± 28 kg were randomly distributed in a completely randomized design where they received diets in which Tifton 85 (Cynodon sp.) hay was replaced with passion fruit by-product (0, 12, 24 and 36%, as-fed basis). The CO, LIPE®, NANOLIPE® and iNDF markers did not differ from the total collection method (p > 0.05) in the estimation of fecal output and nutrient digestibility. The TD and iDM markers overestimated, while iADF underestimated fecal output. Under the presented conditions, we recommend using the CO, LIPE®, NANOLIPE® and iNDF markers to estimate fecal output and nutrient digestibility in diets with inclusion of passion fruit by-product for dairy heifers.

Digestibility, ingestive behavior, and nitrogen balance in goat kids fed a diet containing dehydrated passion fruit residue

The objective was to determine the optimal dehydrated passion fruit pulp replacing ground corn in diets for goat kids. We used 24 crossbred castrated goat kids with initial body weight of 26.6±0.3 kg. The experimental period was 23 days, with 15 days of adjustment and acclimatization (mostly to the installation and diet). Kids were distributed in a randomized experimental design with four treatments of 0, 80, 160, or 240 g/kg of dehydrated passion fruit residue as a supplementary inclusion in the dry matter (DM) content of the diet. The statistical model considered the fixed effect of the included amount of residue from dehydrated passion fruit. Analysis of variance was conducted using the PROC GLM of SAS. Intakes of DM and total digestible nutrients (TDN) were not affected by residue inclusion. However, neutral detergent fiber (NDF) intake increased linearly. The inclusion linearly decreased intake of ether extract (EE) and non-fibrous carbohydrates (NFC). Crude protein (CP) intake was quadratically affected, with a greater value under treatment of 160 g/kg passion fruit. Dry matter and NFC digestibility decreased linearly with the amount of passion fruit included in diet. Fecal N and total N excretion values (in g/d and %N intake) increased linearly. Nitrogen balance and N retained values showed a quadratic increase, with greater values under the 160 g/kg inclusion. Time spent ruminating, DM and NDF eating efficiency, and g DM/bolus chewed were affected quadratically by inclusion of passion fruit residue. The replacement of ground corn with dehydrated passion fruit residue at a level of 160 g/kg is recommended for diets of goat kids to improve their NDF and CP intake and their N balance without affecting DM and TDN intakes.

Substituting corn grain for passion fruit peels in feed for confined sheep / Substituição do grão de milho pela casca de maracujá na alimentação de ovinos confinados

The aim of this study was to evaluate diets substituting corn grain with passion fruit peels on the consumption, ingestive behavior, digestibility of diets and sheep performance. A total of 20 sheep were used, confined in individual pens, fed twice a day, with diets which included passion fruit peels in the proportions of 0; 25; 50; 75 and 100% replacing corn grain. The corn grain represented 70% of the dry matter of the diet and the passion fruit peels were supplied in dehydrated form. The experimental design was completely randomized with five treatments and four replicates. The data were submitted to analysis of variance and regression analysis at the significance level of 5%. There was a linear increase in dry matter consumption with an increase of 3.89 g for each inclusion level of passion fruit peel. The apparent digestibility of dry matter decreased and neutral detergent fiber increased linearly with the substitution of corn grain with passion fruit peels. The feeding, rumination, leisure and water intake times presented averages of 3.74; 2.92; 16.92 and 0.42 hours, and were not influenced by the diets (P>0.05). The average daily weight gain was similar for the animals in the different diets and consequently the final weight presented average values of 0.226 kg day-1 and 32.1 kg, respectively. The inclusion of passion fruit peels in the sheep diet is an interesting alternative because even with the reduced dry matter digestibility, it increased the consumption and promoted similar weight gains.(AU)

Objetivou-se avaliar dietas com substituição do grão de milho pela casca de maracujá sobre o consumo, comportamento ingestivo, digestibilidade das dietas e desempenho de ovinos. Foram utilizados 20 ovinos, confinados em baias individuais, alimentados duas vezes ao dia, com dietas que receberam a inclusão da casca de maracujá nas proporções de 0; 25; 50; 75 e 100% em substituição ao grão de milho. O grão de milho representou 70% da matéria seca da dieta e a casca de maracujá foi fornecida na forma desidratada. O delineamento experimental foi inteiramente casualizado, com cinco tratamentos e quatro repetições. Os dados foram submetidos à análise de variância e análise de regressão, ao nível de 5%. Observou-se um aumento linear do consumo de matéria seca, com acréscimo de 3,89 g para cada nível de inclusão de casca de maracujá. A digestibilidade aparente da matéria seca reduziu e a fibra detergente neutro aumentou linearmente com a substituição ao grão de milho pela casca de maracujá. O tempo de alimentação, ruminação, ócio e ingestão de água, com médias de 3,74; 2,92; 16,92 e 0,42 horas, não foram influenciados pelas dietas (P>0,05). O ganho de peso médio diário foi semelhante para os animais nas diferentes dietas e por consequência o peso final, apresentando valores médios de 0,226 kg dia-1 e 32,1 kg, respectivamente. A inclusão de casca de maracujá na dieta de ovinos é uma alternativa interessante, pois mesmo com a redução da digestibilidade da matéria seca, aumentou o consumo e promoveu semelhante ganho de peso.(AU)