Complexo enzimático em rações para leitões nas fases pré-inicial e inicial / Enzymatic complex in piglet feeds in the pre-initial and initial phases

Objetivou-se, com este trabalho, avaliar o efeito da utilização de complexo enzimático em rações para leitões sobre o desempenho zootécnico nas fases pré-inicial e inicial. Foram utilizados, para isso, 292 leitões, machos castrados e fêmeas, de linhagem comercial. O período de suplementação da enzima, na maternidade, foi do sétimo ao vigésimo primeiro dia de idade, e, na creche, foi do desmame até a saída para a fase de crescimento. O delineamento utilizado foi inteiramente casualizado, sendo a matriz e sua leitegada consideradas a unidade experimental durante a maternidade e uma baia com dez leitões na creche. Na maternidade, os tratamentos consistiram em: T1 - ração controle, sem complexo enzimático; T2 - ração controle com adição on top do complexo enzimático com inclusão de 5%. Na creche, foram definidos três tratamentos: T1 - ração controle; T2 - ração controle com inclusão on top de 5% do complexo enzimático na fase de maternidade; T3 - ração controle com inclusão de 5% de complexo enzimático nas fases de maternidade e creche. A suplementação durante a lactação não influenciou o desempenho da leitegada na maternidade até o 21º dia. Na creche, leitões alimentados com ração controle apresentaram maior peso inicial em relação aos suplementados na fase de maternidade e creche. Ao término da fase, os leitões mostraram desempenho semelhante, independente da dieta. Conclui-se que a inclusão do complexo enzimático na forma on top em dietas para leitões não influenciou parâmetros de desempenho na maternidade, porém, favoreceu a recuperação de leitões desmamados com menor peso.

This study aimed to evaluate the effect of using an enzymatic complex in piglet feeds on the zootechnical performance in the pre-starter and initial phases. For this purpose, 292 piglets, castrated males and females, of commercial lineage were used. The enzyme supplementation period in the maternity hospital was from the seventh to the twenty-first day of age, and in the nursery, it was from the waning until the exit for the growth phase. The design used was completely randomized, with the mother and her litter considered the experimental unit during maternity and a stall with 10 piglets in the nursery. In maternity, the treatments consisted of: T1 - control feed, without the enzymatic complex; T2 - control diet with on top addition of the enzymatic complex with 5% inclusion. Three treatments were defined at the nursery: T1 - control feed; T2 - control diet with inclusion on top of 5% of the enzymatic complex in the maternity phase; T3 - control feed with the inclusion of 5% enzymatic complex in the maternity and nursery phases. Supplementation during lactation did not influence litter performance in the maternity unit until 21 days. In the nursery, piglets fed with control feed had higher initial weight compared to those supplemented in the maternity and nursery phases. At the end of the phase, the piglets showed similar performance, regardless of diet. It is concluded that the inclusion of the enzymatic complex used in the on top form in diets for piglets did not influence performance parameters in maternity; however, it favored the recovery of weaned piglets with lower weight.

Oregano extract (Origanum vulgare) in female broiler chickens of free-range strain raised in the Western Amazon / Extrato de orégano (Origanum vulgare) em frangos de corte fêmeas de linhagem caipira criadas na Amazônia Ocidental

This study aimed to evaluate the effect of oregano extract added to commercial diets on zootechnical performance parameters, carcass yield, immunological conditions, morphometry, and intestinal pH for free-range broiler chickens raised under sanitary challenge conditions. Three hundred chicks of the strain 'Heavy red' were used and distributed in a completely randomized experimental design with five treatments and six replications, totaling 30 experimental units, each consisting of 10 birds. The treatments were: T1: Basal diet without oregano extract (OE); T2: Basal diet with OE (150 mg/kg); T3: Basal diet with OE (250 mg/kg); T4: Basal diet with OE (350 mg/kg); T5: and Basal diet with OE (450 mg/kg). There was a significant effect on feed intake, weight gain, feed conversion, feed efficiency, heterophil/lymphocyte ratio (H/L), and intestinal morphometry. No significant differences were observed in viability, carcass yield, intestinal pH, lymphoid organs, gizzard, heart, and intestine. Oregano extract influenced the liver yield and abdominal fat of broilers. Overall, the inclusion of oregano extract at the level of 350 mg/kg provided better results.(AU)

O objetivo desse trabalho foi avaliar o efeito do extrato de orégano adicionado à ração comercial, considerando os parâmetros de desempenho zootécnico, rendimento de carcaça, condições imunológicas, morfometria e pH intestinal de frangos de corte de linhagem caipira, criados sob condição de desafio sanitário. Foram utilizados 300 pintos da linhagem vermelho pesadão, que foram distribuídos em um delineamento experimental inteiramente casualizado - DIC, com cinco tratamentos e seis repetições, totalizando 30 unidades experimentais, constituídas por 10 aves cada. Os tratamentos foram: T1: Ração basal, sem Extrato de Orégano (EO); T2: Ração basal com EO (150 mg/Kg); T3: Ração basal com EO (250 mg/Kg); T4: Ração basal com EO(350 mg/Kg); T5: Ração basal com EO (450 mg/Kg). Houve efeito significativo no consumo de ração, ganho de peso, conversão alimentar, eficiência alimentar, relação heterofilo/linfócito (H/L) e morfometria intestinal. Não foram observadas diferenças significativas na viabilidade, no rendimento de carcaça, no pH intestinal, nos órgãos linfoides, moela, coração e intestino. Promoveu influência no rendimento de fígado e gordura abdominal dos frangos. A inclusão no nível de 350mg/kg proporciona melhores resultados.(AU)

Efficacy of acidified phytase supplemented cottonseed meal based diets on growth performance and proximate composition of Labeo rohita fingerlings

Abstract The growth of aquaculture sector is strongly dependent upon the continuous supply of inexpensive fish feed with balanced nutritional profile. However, fish meal (FM) is unable to satisfy this demand due to its scarce supply and high cost. In order to test the potential of cottonseed meal (CSM) as a fish meal replacer, a feeding trial of 12 weeks was conducted to check growth performance and proximate composition of Labeo rohita fingerlings. The protein ration of the test feed was satisfied by replacing FM with CSM at 0, 25, 50 and 75%. Sixteen test diets viz., TD1 (control), TD2, TD3, TD4, TD5, TD6, TD7, TD8, TD9, TD10, TD11, TD12, TD13, TD14, TD15 and TD16 were supplemented with citric acid (CA; 0 and 2.5%) and phytase (PHY; 0 and 750 FTU/kg) in a completely randomized design with 3×3 factorial arrangement. The highest weight gain (11.03g), weight gain% (249.21%), specific growth rate (1.39) and best feed conversion ratio (1.20) were recorded by fish fed with TD12. Furthermore, the same level increased the crude protein (59.26%) and fat (16.04%) being significantly different (p 0.05) than that of control. Conclusively, the addition of acidified phytase (CA; 2.5%, PHY; 750 FTU/kg) in TD12 (CSM=50%) led to the improved growth and proximate composition of L. rohita fingerlings.

Resumo O crescimento do setor de aquicultura é fortemente dependente do fornecimento contínuo de rações baratas para peixes com perfil nutricional equilibrado. Porém, a farinha de peixe (FM) não consegue atender a essa demanda devido à sua escassa oferta e alto custo. Com o objetivo de testar o potencial da farinha de semente de canola (MSC) como substituto da farinha de peixe, um ensaio alimentar de 12 semanas foi conduzido para verificar o desempenho de crescimento e a composição centesimal de alevinos de Labeo rohita. A ração de proteína da ração teste foi satisfeita substituindo FM por CSM em 0, 25, 50 e 75%. Dezesseis dietas de teste, viz., TD1 (controle), TD2, TD3, TD4, TD5, TD6, TD7, TD8, TD9, TD10, TD11, TD12, TD13, TD14, TD15 e TD16 foram suplementadas com ácido cítrico (CA; 0 e 2,5%) e fitase (PHY; 0 e 750 FTU / kg) em um delineamento inteiramente casualizado com arranjo fatorial 3 × 3. O maior ganho de peso (11,03g), % de ganho de peso (249,21%), taxa de crescimento específico (1,39) e melhor taxa de conversão alimentar (1,20) foram registrados por peixes alimentados com TD12. Além disso, o mesmo nível aumentou a proteína bruta (59,26%) e a gordura (16,04%), sendo significativamente diferente (p 0,05) do controle. Conclusivamente, a adição de fitase acidificada (CA; 2,5%, PHY; 750 FTU / kg) em TD12 (CSM = 50%) levou a um melhor crescimento e composição próxima de alevinos de L. rohita.

Effect of Housing Design and Location on Production and Economic Performance of Broiler Chickens during Summer in Botswana

Botswana experiences long, hot summer periods which negatively affect broiler productivity and results in economic losses. To determine these negative effects, two parallel broiler production studies were conducted in the North eastern (NE) and South eastern (SE) regions. In each region, three large scale commercial broiler farms were randomly selected based on similarities in bird management and housing systems. In each farm, one house type (Gable, Hoop and See-saw) was selected for long term flock monitoring (1 to 35 days) over three production cycles. Results showed that the production performance of the broilers in the SE region was superior to that in the NE region, with temperatures on being average higher in the NE than in the SE. The European Production Efficiency Factor (EPEF) was significantly higher (p0.05) between the regions at 9.0% and 7.4% for the NE and SE, respectively. In the NE, the Gable structure had the highest profitability and economic efficiency and was thus superior in comparison to the other house structures.(AU)

Efficacy of acidified phytase supplemented cottonseed meal based diets on growth performance and proximate composition of Labeo rohita fingerlings / Eficácia de dietas à base de algodão com fitase acidificada suplementada no desempenho do crescimento e na composição proximada de dedos de Labeo rohita

Abstract The growth of aquaculture sector is strongly dependent upon the continuous supply of inexpensive fish feed with balanced nutritional profile. However, fish meal (FM) is unable to satisfy this demand due to its scarce supply and high cost. In order to test the potential of cottonseed meal (CSM) as a fish meal replacer, a feeding trial of 12 weeks was conducted to check growth performance and proximate composition of Labeo rohita fingerlings. The protein ration of the test feed was satisfied by replacing FM with CSM at 0, 25, 50 and 75%. Sixteen test diets viz., TD1 (control), TD2, TD3, TD4, TD5, TD6, TD7, TD8, TD9, TD10, TD11, TD12, TD13, TD14, TD15 and TD16 were supplemented with citric acid (CA; 0 and 2.5%) and phytase (PHY; 0 and 750 FTU/kg) in a completely randomized design with 3×3 factorial arrangement. The highest weight gain (11.03g), weight gain% (249.21%), specific growth rate (1.39) and best feed conversion ratio (1.20) were recorded by fish fed with TD12. Furthermore, the same level increased the crude protein (59.26%) and fat (16.04%) being significantly different (p<0.05) than that of control. Conclusively, the addition of acidified phytase (CA; 2.5%, PHY; 750 FTU/kg) in TD12 (CSM=50%) led to the improved growth and proximate composition of L. rohita fingerlings.

Resumo O crescimento do setor de aquicultura é fortemente dependente do fornecimento contínuo de rações baratas para peixes com perfil nutricional equilibrado. Porém, a farinha de peixe (FM) não consegue atender a essa demanda devido à sua escassa oferta e alto custo. Com o objetivo de testar o potencial da farinha de semente de canola (MSC) como substituto da farinha de peixe, um ensaio alimentar de 12 semanas foi conduzido para verificar o desempenho de crescimento e a composição centesimal de alevinos de Labeo rohita. A ração de proteína da ração teste foi satisfeita substituindo FM por CSM em 0, 25, 50 e 75%. Dezesseis dietas de teste, viz., TD1 (controle), TD2, TD3, TD4, TD5, TD6, TD7, TD8, TD9, TD10, TD11, TD12, TD13, TD14, TD15 e TD16 foram suplementadas com ácido cítrico (CA; 0 e 2,5%) e fitase (PHY; 0 e 750 FTU / kg) em um delineamento inteiramente casualizado com arranjo fatorial 3 × 3. O maior ganho de peso (11,03g), % de ganho de peso (249,21%), taxa de crescimento específico (1,39) e melhor taxa de conversão alimentar (1,20) foram registrados por peixes alimentados com TD12. Além disso, o mesmo nível aumentou a proteína bruta (59,26%) e a gordura (16,04%), sendo significativamente diferente (p <0,05) do controle. Conclusivamente, a adição de fitase acidificada (CA; 2,5%, PHY; 750 FTU / kg) em TD12 (CSM = 50%) levou a um melhor crescimento e composição próxima de alevinos de L. rohita.

Detection of microorganisms with antibacterial activities from different industrial wastes and GC-MS analysis of crude microbial extract

Abstract Many soil microorganisms i.e., bacteria and fungi produce secondary metabolites called antibiotics. These are used for the treatment of some of the bacterial, fungal and protozoal diseases of humans. There is a need for isolation of a broad spectrum of antibiotics from microorganisms due to the emergence of antibiotic resistance. In the present study two antibiotic producing bacteria Klebsiella pneumoniae and Bacillus cereus were isolated from pharmaceutical and poultry feed industry of Hattar, Haripur Pakistan. Total 10 waste samples were collected from different industries (Marble, Ghee, Soap, Mineral, Steel, Poultry Feed, Pharmaceutical, Qarshi, Cosmetic and Glass). Thirty-three bacterial strains were isolated from industrial wastes of these ten different industries. Fourteen out of thirty-three bacterial strains exhibited antimicrobial activities against at least one of the test microbes considered in this study including Escherchia coli, Staphylococcus aureus and Salmonella typhi. The bacteria were isolated by standard serial dilution spread plate technique. Morphological characterization of the isolates was done by Gram staining. Nine bacterial isolates out of fourteen were initially identified as B. cereus and five as K. pneumoniae through biochemical characterization. The antibacterial activities were tested by well diffusion method. Maximum number of antibiotic producing bacteria were isolated from pharmaceutical and poultry feed industry based on the results of primary screening, the most potential isolates S9, S19, S20, S22 and S23 were selected for secondary screening. The maximum activity against E. coli and S. aureus was recorded by bacterial isolate S19 i.e zones of inhibition of 6.5mm and 9mm while S20 showed 7.5mm and 6mm zones respectively. Molecular identification was carried out on the basis of 16S rRNA sequence analysis. Finally, the isolates were identified as B. cereus accession number LC538271and K. pneumoniae accession number MT078679. Analysis of bacterial extract S20 through GC-MS indicated the presence of 8 compounds of diverse nature and structure. Present study suggests that wastes of pharmaceutical and poultry feed industry may have antibiotic producing bacteria. These bacteria could be utilized for the production of antibiotics. B. cereus and K. pneumoniae isolated from wastes of poultry feed and pharmaceutical industries have the potential to produce antibiotics and could be used to control the microbial growth.

Resumo Muitos microrganismos do solo, ou seja, bactérias e fungos produzem metabólitos secundários chamados antibióticos. Eles são usados para tratamento de algumas doenças bacterianas, fúngicas e protozoárias em humanos. Há necessidade de isolamento de um amplo espectro de antibióticos de microrganismos devido ao surgimento de resistência aos antibióticos. No presente estudo, duas bactérias produtoras de antibióticos, Klebsiella pneumoniae e Bacillus cereus, foram isoladas da indústria farmacêutica e de ração avícola de Hattar, Haripur, Paquistão. Um total de 10 amostras de resíduos foi coletado de diferentes indústrias (mármore, ghee, sabão, mineral, aço, ração para aves, farmacêutica, Qarshi, cosmética e vidro). Trinta e três cepas bacterianas foram isoladas de resíduos industriais dessas dez diferentes indústrias. Quatorze das 33 cepas bacterianas exibiram atividades antimicrobianas contra pelo menos um dos micróbios de teste considerados neste estudo, incluindo Escherchia coli, Staphylococcus aureus e Salmonella typhi. As bactérias foram isoladas pela técnica de placa de diluição em série padrão. A caracterização morfológica dos isolados foi feita por coloração de gram. Nove isolados bacterianos de 14 foram inicialmente identificados como B. cereus e cinco como K. pneumoniae por meio de caracterização bioquímica. As atividades antibacterianas foram testadas pelo método de difusão em poço. O número máximo de bactérias produtoras de antibióticos foi isolado da indústria farmacêutica e de ração avícola com base nos resultados da triagem primária, os isolados mais potenciais S9, S19, S20, S22 e S23 foram selecionados para a triagem secundária. A atividade máxima contra E. coli e S. aureus foi registrada pelo isolado bacteriano S19, ou seja, zonas de inibição de 6,5 mm e 9 mm, enquanto S20 mostrou zonas de 7,5 mm e 6 mm, respectivamente. A identificação molecular foi realizada com base na análise da sequência 16S rRNA. Finalmente, os isolados foram identificados como B. cereus número de acesso LC538271 e K. pneumoniae número de acesso MT078679. A análise do extrato bacteriano S20 por meio de GC-MS indicou a presença de oito compostos de natureza e estrutura diversas. O presente estudo sugere que resíduos da indústria farmacêutica e de ração para aves podem conter bactérias produtoras de antibióticos. Essas bactérias podem ser utilizadas para a produção de antibióticos B. cereus e K. pneumoniae isolados de resíduos de rações de aves e indústrias farmacêuticas têm potencial para produzir antibióticos e podem ser usados para controlar o crescimento microbiano.

The effect of feeding frequency on the performance of juvenile cobia (Rachycentron canadum) / Efeito da frequência alimentar no desempenho de juvenis do cobia (Rachycentron canadum)

Para ajustar a frequência alimentar de juvenis de cobia ou beijupirá (Rachycentron canadum), 540 juvenis, com peso médio de 2,0 g (± 0,4) e comprimento médio de 8,7cm (± 0,5), foram distribuídos em quatro tratamentos: T2, T4, T6 e T8 (alimentação realizada duas, quatro, seis e oito vezes ao dia, respectivamente). Cada tratamento teve três repetições. Os juvenis de beijupirá foram alimentados em regime ad libitum. Observou-se diferença significativa no peso final (P<0,05), tendo os peixes do tratamento que receberam ração duas vezes ao dia (T2) apresentado peso significativamente menor do que os peixes que receberam oito refeições ao dia (T8). Não houve diferença nas variáveis conversão alimentar, ganho de peso, comprimento e sobrevivência (P>0,05) entre os tratamentos. Assim, para juvenis de beijupirá, observou-se que a frequência de alimentação de oito refeições por dia, juntamente com um regime de alimentação ad libitum, é a mais adequada durante essa fase. Os dados obtidos no presente experimento permitem otimizar uma etapa da cadeia produtiva do beijupirá.

Enzymatic hydrolysis of lignocellulosic residues and bromatological characterization for animal feed / Hidrólise enzimática de resíduos lignocelulósicos e caracterização bromatológica para ração animal

ABSTRACT: This study evaluated the action of commercial and non-commercial cellulases and pectinases in the hydrolysis of soybean hulls (SH) and corn stover and cobs (CSC), the effect of temperature and agitation on the lignocellulosic substrate hydrolysis and the bromatological characteristics of hydrolyzed substrates. The effect of pretreatment on the hydrolysis of lignocellulosic residues and bromatological analysis were also evaluated. The highest hydrolytic activity occurred at 300 rpm for SH (47.95 and 51.43% for cellulase and pectinase, respectively) and at 350 rpm for CSC (26.05 and 9.23% for cellulase and pectinase, respectively). Non-commercial enzymes achieved 7.26-30% of the amount of hydrolysis obtained with commercial enzymes, on the same substrates. Pretreatment with 7.5% of NaOH and a particle size of the substrate of 0.5 mm significantly increased the hydrolysis of SH and CSC for both enzymes. The bromatological characteristics showed that soybean hulls hydrolyzed with both commercial cellulase and pectinase have potential for large-scale use in animal feed production.

RESUMO: Foram avaliadas a ação de celulases e pectinases comerciais e não comerciais na hidrólise de casca de soja (CS) e palha e espigas de milho (PEM), o efeito da temperatura e da agitação na hidrólise do substrato lignocelulósico e as características bromatológicas dos substratos hidrolisados. O efeito do pré-tratamento na hidrólise de resíduos lignocelulósicos e a análise bromatológica também foram avaliados. A maior atividade hidrolítica ocorreu a 300 rpm para CS (47,95 e 51,43% para celulase e pectinase, respectivamente) e a 350 rpm para PEM (26,05 e 9,23% para celulase e pectinase, respectivamente). As enzimas não comerciais atingiram 7,26-30% da quantidade de hidrólise obtida com as enzimas comerciais, nos mesmos substratos. O pré-tratamento com 7,5% de NaOH e um tamanho de partícula do substrato de 0,5 mm aumentou significativamente a hidrólise de CS e PEM para ambas as enzimas. As características bromatológicas mostraram que a casca de soja hidrolisada com celulase e pectinase comercial tem potencial para uso em larga escala na produção de ração animal.

Effects of mixed feeding strategies with different dietary energy: protein ratios on juvenile Nile tilapia (Oreochromis niloticus) / Efeitos de estratégias de alimentação mista com diferentes relações de energia: proteína para juvenis de tilápia-do-Nilo (Oreochromis niloticus)

The evaluation of feeding strategies is necessary to ensure the sustainability of aquaculture. This study assessed the effect of two diets with different E:P ratios (9.6 and 10.3 kcal of digestible energy per gram of crude protein) on Nile tilapia juveniles. The growth, feed and nutrient use, economic parameters, whole-body composition, and liver steatosis of fish were evaluated. There was no significant effect of treatments on the growth, feed intake, feed conversion ratio, uniformity, and survival of the fish. The lower feed cost (P>0.05) per biomass or 1000 units produced was registered in Nile tilapia juveniles fed with 10.3 kcal DE/g CP diet for seven days. The contribution of ether extract in fish weight gain was reduced (P=0.055) by the increased use of the 10.3 kcal DE/g CP diet in the feeding strategies. The same trend was observed in fish whole-body lipid levels. Body indexes were similar (P>0.05) among fish from the different treatments. Mixing diets with different E:P ratios in a weekly feeding protocol does not impair productive performance of Nile tilapia juveniles. However, considering the cost of feeding, the recommendation is to supply a diet with 33%CPand 3.4 kcal/DE for seven days per week.(AU)

A avaliação das estratégias de alimentação é necessária para garantir a sustentabilidade da aquicultura. Este estudo avaliou o efeito de duas dietas com diferentes proporções de E:P (9,6 e 10,3 kcal de energia digestível por grama de proteína bruta) para juvenis de tilápia do Nilo. Foram avaliados o crescimento, uso da dieta e nutrientes, parâmetros econômicos, composição corporal e esteatose hepática de peixes. Não houve efeito significativo dos tratamentos sobre o crescimento, consumo de ração, conversão alimentar, uniformidade e sobrevivência dos peixes. O menor custo de ração (P>0,05) por biomassa ou 1000 unidades produzidas foi registrado nos juvenis de tilápia do Nilo alimentados com a dieta 10,3 kcal DE/g PB por sete dias. A contribuição do extrato etéreo no ganho de peso dos peixes foi reduzida (P=0,055) pela maior utilização da dieta 10,3 kcal DE/g PB nas estratégias de alimentação utilizadas. A mesma tendência foi observada nos níveis de lipídios corporais em peixes. Os índices corporais foram semelhantes (P>0,05) entre os peixes dos diferentes tratamentos. A mistura de dietas com diferentes relações E:P em um protocolo de alimentação semanal não prejudica o desempenho produtivo de juvenis de tilápia do Nilo. No entanto, considerando os custos de alimentação, a recomendação é fornecer uma dieta com 33% PB e 3,4 kcal/DE por sete dias por semana.(AU)

Effect of adding Spirulina platensis algae to small ruminant rations on productive, reproductive traits and some blood components

This experiment conducted using 20 Rahmani ewes at the last third of pregnancy in two equal groups. One group served as control, while the other group received Spirulina platensis(SP) at the rate of 0.5 gm 10 kg-1live body weight. The objective was to find out the effect of adding Spirulina platensisalgae to small ruminant rations on reproductive and productive traits and blood components of sheep. The experiments lasted for 120 days for both dams and their lambs after weaning. The findings proved that adding SP in ewes' diets had no effect on the average of live body weight change. Average milk yield was significantly (p <0.01) higher in the treatment group than the control. Lamb's birth weight and daily body gain of the treated group were significantly (p <0.01) higher than the control. Blood and serum picture profile of ewes were significantly higher when fed SP additive than the control.It could be concluded that the addition of SP to the ration of sheep positively preserved their health, productive and reproductive status as well as their lambs' growth rate. Also the additive improved the economic efficiency of treated animals by about 53.13%.(AU)

Carcass characteristics and meat quality of lambs fed diets with different roughage: concentrate ratios supplemented with liquid residue of cassava

The objective of this study was to evaluate the effects of lambs fed two roughage: concentrate (R:C) ratios, with or without the liquid residue of cassava (LRC) on carcass traits and meat quantity. Forty lambs (19.5 ± 1.45 kg body weight), non-castrated, crossbred Santa Inês were distributed in a completely randomized 2 × 2 factorial design. There was no effect (p > 0.05) of the inclusion of LRC on the variables. The roughage:concentrate ratio of 40:60 promoted higher values for empty body weight, hot carcass weight, cold carcass weight, subcutaneous thickness fat, cooling loss, Longissimus muscle area, carcass morphometric measurements, and commercial cuts. Higher weight of leg, muscles, fat, bone and other tissues, as well as for the ratio muscle:fat, and muscle:bone was observed in lambs fed 40R:60C. For the meat chemical composition, there was the effect (p < 0.05) only for ether extract (EE) for lambs fed 40R:60C ratio. The redness (a*) of meat was greater (p < 0.05) for lambs fed 80R:20C. The other physicochemical characteristics were not influenced. The roughage:concentrate ratio of 40:60 improves the carcass traits and commercial cuts, but does not promote changes in meat quality, independent of supplementation with liquid residue of cassava.(AU)

Effect of Dietary Supplementation with Rosemary Complex Powder on the Growth Performance of Native Chickens

This study investigated the effects of dietary supplementation with rosemary complex powder on the growth performance of native chickens. In total, 180 one day-old native chicks were assigned to one of three dietary groups (60 birds each). The control group (Group A) received the basal diet. In addition to the basal diet, the two experimental diets (Groups B and C) were supplemented with 0.2% and 0.4% rosemary complex powder, which contained rosemary leaves, sweet basil, pineapple sage and sweet lavender. Over 19 weeks, feed intake was recorded to determine the average daily gain and feed conversion ratio. In weeks 10 and 15, blood samples were taken for serum antibody titer analysis. At the end of the experiment, serum immunoglobulin A (IgA) and serum immunoglobulin G (IgG) concentrations were examined. No differences were observed among the groups in terms of the starting weight, weight in week 19, average daily feed intake, average daily gain, or average feed conversion ratio. The addition of rosemary complex powder improved total weight gain by 1.52%. Serum IgA and serum IgG concentrations were significantly lower in the experimental groups in comparison to the control group (p<0.05). Villus height, villus width, crypt depth, and villus height/crypt depth ratio were significantly higher in group B than in group A (p<0.05). In summary, rosemary complex powder improved the intestinal absorption capacity of chickens and significantly reduced their immunoglobulin concentrations.(AU)

Effect of Turmeric (Curcuma Longa) on Duodenal Structure in Broiler Chickens

Supplementation of feed with turmeric (Curcuma longa) has been shown to be beneficial in poultry farming. The present study aimed at evaluating the effect of turmeric on the duodenal structure of broiler chickens during the first 42 days of life. A control and three treatment groups (turmeric powder added to feed at the following doses: E1 - 5; E2 - 10; E3 - 20 g/kg feed) were constituted (n = 8). Addition of turmeric powder in the feed resulted in an increase in intestinal villi height and a decrease in crypts depth in case of groups E1 and E2, while the villus height to crypt depth ratio generally did not differ significantly from the control. Turmeric also influenced the histological structure of the duodenum, as well as the presence of IL-6 and TNFα, as evidenced through staining. Addition of 0.5 and 1 % turmeric powder in the feed had evident results on body weight gain.(AU)

Insect meal in poultry feed: a potential protein source

The present study aims to conduct a review on the potential use of insect meal as an alternative protein source in poultry feed, particularly to serve the rearing of free-range chickens. Insects are already part of the diet of birds in their natural habitat, and the availability of low-cost alternative foods with low environmental impact is essential for the development of the activity. The review comprehended studies that used meals consisting of silkworm (Bombyx mori) chrysalis, earthworm (Eisenia foetida), housefly (Musca domestica), black soldier fly (Hermetia illucens) and mealworm beetle (Tenebrio molitor) to replace plant-based protein sources. In general, insect meals have a high content of crude protein and ether extract, as well as an essential amino acid profile suitable for poultry feeding. The addition of insect meal in poultry feed normally shows good results as to growth performance and egg production, without causing a negative effect on carcass characteristics, meat sensory quality and egg quality, presenting itself as an alternative protein source with good prospects for replacing plant-based sources in poultry feed.(AU)

Impact of reference diet composition on apparent digestibility coefficients of two protein-rich ingredients in Nile tilapia

Protein quality is related to amino acid composition and digestibility. Accurate evaluation of apparent digestibility coefficients (ADCs) of nutrients in commonly used feedstuffs is paramount for formulating efficient aquafeed. ADCs of soybean meal (SBM) and poultry by-product meal (PBM) were evaluated using reference diets formulated with two types of ingredients (semi-purified [SP] and practical [P]) for juvenile Nile tilapia ( Oreochromis niloticus, Linnaeus) of the GIFT strain. Groups of 20 juveniles (65.05 ± 12.37 g) were fed twice a day to apparent satiety with one of the four experimental diets (SBM-SP, SBM-P, PBM-SP, and PBM-P) in quadruplicate for 30 days. After the last feeding, feces were collected by siphoning hourly and the ADCs of dry matter, protein, and amino acids (AAs) were calculated. Nile tilapia exhibited a high capacity to digest SBM and PBM, with most ADCs exceeding 90 %. The type of reference diet affected the ADCs of protein and AAs on the test ingredients, with the SP reference diet providing the highest ADC, mainly in SBM. Digestibility data generated with a P-type reference diet demonstrated more practical relevance than those generated with an SP-type reference diet. They can be applied in digestibility studies for Nile tilapia.(AU)

Performance and Carcass of Broiler Quails Fed Distillers' Dried Grains with Solubles (DDGS)

This study determined the metabolizable energy of DDGS for broiler quails (Experiment I) and evaluated the effect of different dietary levels of DDGS on performance, carcass, organ weight, meat quality, and economic viability (Experiment II). In Experiment I, 72 broiler quails were randomly distributed into two treatments (reference or test diets). The experimental period consisted of 5 days of adaptation, followed by 5 days of total excreta collection. Experimental diets consisted of a reference or a test diet containing 800 g/kg reference diet and 200 g/kg DDGS. In experiment II, 432 unsexed broiler quails were randomly distributed into groups fed six levels of DDGS (0, 50, 100, 150, 200, or 250 g/kg). At 43 days of age, birds were slaughtered and evaluated for carcass yield, organ weight, and meat quality. Apparent metabolizable energy values corrected for nitrogen retention of DDGS were 2,488 and 2,466 kcal/kg for males and females, respectively. In the growth phase and the overall period, feed intake increased linearly (p=0.015 and 0.040) and feed conversion ratio worsened (p=0.038 and 0.001) with the inclusion of DDGS in the diet, respectively. A linear increasing (p=0.001) of gizzard weight was observed with increasing dietary DDGS levels, while the economic variables were affect depending on seasonal feedstocks prices. It is concluded that dietary levels up to 250 g/kg DDGS do not affect growth, carcass characteristics, and meat quality of broiler quails. However, the prices of ingredients in the harvest and off-season period should be considered to determine the level of inclusion of DDGS.(AU)

Commercial Feed Diluted with Different Fiber Sources and Enzyme Product for Broilers: Growth Performance, Carcass and Gut Health

The dilution of commercial broiler feed with copra meal (CM), palm kernel meal (PKM) and cassava leaf meal (CLM) and enzyme supplementation was investigated. Two hundred and eighty 7-day old Cobb 500 broilers were fed commercial feed alone or diluted with the test ingredients with and without enzyme. There were no interaction effects (p>0.05) on feed intake (FI) and weight gain (WG). Poorer (p<0.05) feed conversion ratio (FCR) was observed on the diet diluted diets from 22-42 d, but this was restored (p>0.05) by enzyme supplementation. There was no interaction effect on final body weight (FBW). Diet dilution depressed FBW (p<0.05) but enzyme supplementation restored (p>0.05) this weight depression. Abdominal fat was lighter (p<0.05) on CLM compared to CM. There were no interaction effects (p>0.05) on the weight of gut segments and E. coli count. Heavier (p<0.05) liver, gizzard and intestine were observed on the test diets. Commercial feed dilution with CM, CLM and PKM at 100 g/kg for starter and 200 g/kg for finisher would be a viable option for smallholder broiler production. More research is needed into dilution levels and enzyme concentrations.(AU)

The Effects of Yucca Schidigera Extract and Multi-carbohydrase in Different Crude Protein Diets on Growth Performance, Nutrient Digestibility, Carcass Parameters and Excreta Noxious Gas Contents in Broilers

The scarcity and increase in the price of animal feeds have attracted the attention of nutritionists to address this issue. The inclusion of plant extracts and enzymes to protein-reduced diet could be a feasible strategy to reducing the feed cost. Therefore, the purpose of this study was to assess the impacts of Yucca schidigera extract and multi-carbohydrase in low crude protein (CP) diets of broiler on growth performance, nutrient digestibility, carcass metrics, and noxious gas levels in the excreta. A total of 480, 1-d-old ROSS 308 were randomly allocated into 4 dietary treatments, six replication, and 20 birds/cage. Phase 1, T1(CP 21%, ME 2,969 kcal/kg); T2 (CP 19%, ME 2,863 kcal/kg + 0.02% Yucca); T3 (CP 17%, ME 2,865 kcal/kg + 0.02% Yucca); T4 (CP 17%, ME 2,861 kcal/kg + 0.02% Yucca + 0.1% multi-carbohydrase). Phase 2, T1 (CP 19%, ME 3,086 kcal/kg); T2 (CP 17%, ME 2,977 kcal/kg + 0.02% Yucca); T3 (CP 15%, ME 2,978 kcal/kg + 0.02% Yucca); T4 (CP 15%, ME 2,978 kcal/kg + 0.02% Yucca + 0.1% 0.1% multi-carbohydrase). Although the addition of YS and multi-carbohydrase to the low CP diets on the growth performance did not improve, it revealed the positive result on the nutrient digestibility, carcass parameters, and noxious gas emission. Overall, broilers supplemented with YS 0.02% and multi-carbohydrase (0.1%) demonstrated the best production performances compared to the other treatment groups. Thus, a combination of YS and multi-carbohydrase could be added to the diets with low CP to boost broiler production performance.(AU)

Carbohydrase inclusion in a corn-soybean diet improves broiler growth performance

An experiment was conducted to evaluate the effect of diets with reduced energy level content, supplemented with carbohydrase, on broiler performance and the coefficient of metabolizability of nutrients. A total of 720 one-day-old male Cobb-500 chicks were distributed in a completely randomized design, with six treatments, eight repetitions of 15 birds each. The treatments were: (1) a positive control, basal diet to meet the requirements of broiler chickens (PC); (2) a negative control, basal diet with a reduction of 80 kcal kg-1 (NC); (3) NC + alphagalactosydase; (4) NC + xylanase; (5) NC + xylanase and alphagalactosydase, and (6) NC + enzymatic blend (alphagalactosydase, xylanase, pectinase and amylase). The nutrient digestibility was not improved by use of enzymes. At 7 days of age, the broilers which were fed diets supplemented with enzymes showed a lower feed intake (FI) and better feed conversion ratio (FCR) than the broilers fed on PC. Both the NC and enzymatic blend resulted in a worse performance of the broilers at 21, 35 and 42 days old. The use of alphagalactosydase and xylanase, isolated or in combination, in a corn-soybean meal-based diet is effective in improving the growth performance of broilers fed energy-reduced diets.(AU)

Farelo de mamona em dietas para tilápia do Nilo / Castor bean bran in diets for Nile tilapia

No cultivo de peixes, um dos entraves é o gasto com a alimentação, que pode chegar até 70% dos custos de produção. Diante desse fato, os pesquisadores vêm buscando soluções, para minimizar estes custos por meio da substituição de ingredientes tradicionais por alternativos. Neste sentido, o presente estudo visa avaliar a inclusão do farelo de mamona em dietas para alevinos de tilápia do Nilo. Foram utilizados 144 animais com peso médio inicial 0,8 ± 0,01 g, distribuídos em 24 aquários de polietileno, com capacidade para 80L de água, cada um, interligados a um sistema de recirculação, emdelineamento experimental inteiramente casualizado, composto por quatro tratamentos: 0,0; 3,0; 6,0 e 9,0% de farelo de mamona, com seis repetições, cada um. Ao término do período experimental foram avaliados: peso final, comprimento total, taxa de sobrevivência, ganho empeso, consumo de ração, conversão alimentar aparente, taxa de eficiência proteica, fator de condição, e os índices hepatossomático e lipossomático. Ofarelo de mamona apresentou efeito linear decrescente sobre o peso final, comprimento total e ganho empeso. Desta forma, a inclusão do farelo de mamona em dietas para alevinos de tilápia do Nilo comprometeu o ganho empeso destes animais.(AU)

In fish farming, one of the barriers is the cost of food, which can reach up to 70% of production costs. Faced with this fact, researchers have been looking for solutions to minimize these costs by replacing traditional ingredients with alternative ones. In this sense, the present study aims to evaluate the inclusion of castor bean bran in diets for Nile tilapia fingerlings. We used 144 animals with a mean initial weight of 0.8 ± 0.01 g, distributed in 24 polyethylene aquariums, with a capacity of 80L ofwater, connected to a recirculation system, in a completely randomized experimental design, consisting of four treatments: 0.0; 3.0; 6.0 and 9.0% of castor bean bran, with six replications each. At the end of the experimental period, final weight, total length, survival rate, weight gain, feed intake, apparent feed conversion, protein efficiency rate, condition factor, and hepatosomatic and liposomatic index. Castor bean bran showed a decreasing linear effect on final weight, total length and weight gain. Thus, the inclusion of castor bean bran in diets for Nile tilapia fingerlings compromised the weight gain of these animals.(AU)