Fungal cellulases: production by solid-state cultivation in packedbed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse / Celulases fúngicas: produção por cultivo sólido em biorreator de leito empacotado usando resíduos sólidos agroindustriais como substratos e aplicação na hidrólise de bagaço de cana

Cellulases are essential for the hydrolysis of lignocellulosic materials in the production of second generation ethanol. Solid-state cultivation is a process that provides high concentrations of enzymes that can be used in this hydrolysis. The objectives of this work were to produce cellulases by cultivating the fungus Myceliophthora thermophila I-1D3b in a packed bed bioreactor with sugarcane bagasse (SCB) and wheat bran (WB) as substrate and to evaluate the efficiency of the enzymatic extract in the hydrolysis of SCB in natura (BIN) and pretreated with ozone, alkali and ultrasound (BOU). The conditions for enzyme production in the bioreactor were SCB:WB at a ratio of 2.3:1 (w/w), 75 % moisture content; 45 ºC; aeration rate 240 L h-¹ and 96 h. The enzyme production was evaluated by endoglucanase, xylanase, filter paper (FPU) and β-glycosidase activities. For the application of the enzymes, a central composed response surface design with 5 repetitions of the central point was used, taking enzyme volume and hydrolysis time as factors. Such cultivation yielded the following enzymatic activities: 723 U gss-¹ of endoglucanases, 2024 U gss-¹ of xylanase, 12.6 U gss-¹ of FPU and 41 U gss-¹ of β-glucosidase. The results of the application tests indicated the best conditions as 7.0 mL of the enzyme extract (4.2 FPU) and 6 hours for BIN and BOU. The best cellulose-glucose conversions were obtained for BOU, reaching 32.1 % at 65 ºC. In conclusion, the enzyme production in the packed bed bioreactor was efficient and BOU pretreatment improved the hydrolysis of biomass, increasing the efficiency of conversion of cellulose to glucose.

Celulases são essenciais para a hidrólise de materiais lignocelulósicos visando à produção de etanol de segunda geração. O cultivo em estado sólido é um processo que proporciona altas concentrações de enzimas que podem ser aplicadas nessa hidrólise. O objetivo deste trabalho foi produzir celulases pelo fungo Myceliophthora thermophila I-1D3b em biorreator de leito empacotado com bagaço decana (BC) e farelo de trigo (FT) como substrato e avaliar a eficiência deste extrato enzimático na hidrólise de bagaço de cana in natura (BIN) e pré-tratado com ozônio, álcali e ultrassom (BOU). As condições para produção de enzimas no biorreator foram BC:FT numa proporção de 2,3:1 (m/m); 75 %de umidade em base úmida; 45 ºC; taxa de aeração 240 L h-1 e 96 h. A produção de enzimas foi avaliada pelas atividades de endoglucanase, xilanase, papel de filtro (FPU) e β-glicosidase. Para a aplicação das enzimas, foi utilizado um planejamento de superfície de resposta central composto com 5 repetições do ponto central, tendo como fatores o volume de extrato enzimático e o tempo de hidrólise. Esse cultivo produziu as seguintes atividades enzimáticas: 723 U gss-1 de endoglucanases, 2024 U gss-¹ de xilanase, 12,6 U gss-¹ de FPU e 41 U gss-¹ de β-glucosidase. Os resultados da aplicação dessas enzimas na hidrólise indicaram como ótimos 7,0 mL de extrato enzimático (4,2 FPU) e 6 horas para BIN e BOU. As melhores conversões de celulose-glicose foram obtidas para a BOU, atingindo 32,1 % a 65 ºC. Em conclusão, a produção de enzimas no biorreator de leito compactado foi eficiente e o pré-tratamento BOU melhorou a hidrólise da biomassa, aumentando a eficiência de conversão de celulose em glicose.

Fungal cellulases: production by solid-state cultivation in packedbed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse / Celulases fúngicas: produção por cultivo sólido em biorreator de leito empacotado usando resíduos sólidos agroindustriais como substratos e aplicação na hidrólise de bagaço de cana

Cellulases are essential for the hydrolysis of lignocellulosic materials in the production of second generation ethanol. Solid-state cultivation is a process that provides high concentrations of enzymes that can be used in this hydrolysis. The objectives of this work were to produce cellulases by cultivating the fungus Myceliophthora thermophila I-1D3b in a packed bed bioreactor with sugarcane bagasse (SCB) and wheat bran (WB) as substrate and to evaluate the efficiency of the enzymatic extract in the hydrolysis of SCB in natura (BIN) and pretreated with ozone, alkali and ultrasound (BOU). The conditions for enzyme production in the bioreactor were SCB:WB at a ratio of 2.3:1 (w/w), 75 % moisture content; 45 ºC; aeration rate 240 L h-¹ and 96 h. The enzyme production was evaluated by endoglucanase, xylanase, filter paper (FPU) and β-glycosidase activities. For the application of the enzymes, a central composed response surface design with 5 repetitions of the central point was used, taking enzyme volume and hydrolysis time as factors. Such cultivation yielded the following enzymatic activities: 723 U gss-¹ of endoglucanases, 2024 U gss-¹ of xylanase, 12.6 U gss-¹ of FPU and 41 U gss-¹ of β-glucosidase. The results of the application tests indicated the best conditions as 7.0 mL of the enzyme extract (4.2 FPU) and 6 hours for BIN and BOU. The best cellulose-glucose conversions were obtained for BOU, reaching 32.1 % at 65 ºC. In conclusion, the enzyme production in the packed bed bioreactor was efficient and BOU pretreatment improved the hydrolysis of biomass, increasing the efficiency of conversion of cellulose to glucose.(AU)

Celulases são essenciais para a hidrólise de materiais lignocelulósicos visando à produção de etanol de segunda geração. O cultivo em estado sólido é um processo que proporciona altas concentrações de enzimas que podem ser aplicadas nessa hidrólise. O objetivo deste trabalho foi produzir celulases pelo fungo Myceliophthora thermophila I-1D3b em biorreator de leito empacotado com bagaço decana (BC) e farelo de trigo (FT) como substrato e avaliar a eficiência deste extrato enzimático na hidrólise de bagaço de cana in natura (BIN) e pré-tratado com ozônio, álcali e ultrassom (BOU). As condições para produção de enzimas no biorreator foram BC:FT numa proporção de 2,3:1 (m/m); 75 %de umidade em base úmida; 45 ºC; taxa de aeração 240 L h-1 e 96 h. A produção de enzimas foi avaliada pelas atividades de endoglucanase, xilanase, papel de filtro (FPU) e β-glicosidase. Para a aplicação das enzimas, foi utilizado um planejamento de superfície de resposta central composto com 5 repetições do ponto central, tendo como fatores o volume de extrato enzimático e o tempo de hidrólise. Esse cultivo produziu as seguintes atividades enzimáticas: 723 U gss-1 de endoglucanases, 2024 U gss-¹ de xilanase, 12,6 U gss-¹ de FPU e 41 U gss-¹ de β-glucosidase. Os resultados da aplicação dessas enzimas na hidrólise indicaram como ótimos 7,0 mL de extrato enzimático (4,2 FPU) e 6 horas para BIN e BOU. As melhores conversões de celulose-glicose foram obtidas para a BOU, atingindo 32,1 % a 65 ºC. Em conclusão, a produção de enzimas no biorreator de leito compactado foi eficiente e o pré-tratamento BOU melhorou a hidrólise da biomassa, aumentando a eficiência de conversão de celulose em glicose.(AU)

Multi-enzyme complex of white rot fungi in saccharification of lignocellulosic material

The multi-enzyme complex (crude extract) of white rot fungi Pleurotus ostreatus, Pleurotus eryngii, Trametes versicolor, Pycnosporus sanguineus and Phanerochaete chrysosporium were characterized, evaluated in the hydrolysis of pretreated pulps of sorghum straw and compared efficiency with commercial enzyme. Most fungi complexes had better hydrolysis rates compared with purified commercial enzyme.(AU)

Enzymatic saccharification and fermentation of cellulosic date palm wastes to glucose and lactic acid

The bioconversion of cellulosic wastes into high-value bio-products by saccharification and fermentation processes is an important step that can reduce the environmental pollution caused by agricultural wastes. In this study, enzymatic saccharification of treated and untreated date palm cellulosic wastes by the cellulases from Geobacillus stearothermophilus was optimized. The alkaline pre-treatment of the date palm wastes was found to be effective in increasing the saccharification percentage. The maximum rate of saccharification was found at a substrate concentration of 4% and enzyme concentration of 30 FPU/g of substrate. The optimum pH and temperature for the bioconversions were 5.0 and 50 °C, respectively, after 24 h of incubation, with a yield of 31.56 mg/mL of glucose at a saccharification degree of 71.03%. The saccharification was increased to 94.88% by removal of the hydrolysate after 24 h by using a two-step hydrolysis. Significant lactic acid production (27.8 mg/mL) was obtained by separate saccharification and fermentation after 72 h of incubation. The results indicate that production of fermentable sugar and lactic acid is feasible and may reduce environmental pollution by using date palm wastes as a cheap substrate. (AU)

High levels of B-xylosidase in Thermomyces lanuginosus: potential use for saccharification

A new strain of Thermomyces lanuginosus was isolated from the Atlantic Forest biome, and its -xylosidases optimization in response to agro-industrial residues was performed. Using statistical approach as a strategy for optimization, the induction of -xylosidases activity was evaluated in residual corn straw, and improved so that the optimum condition achieved high -xylosidases activities 1003 U/mL. According our known, this study is the first to show so high levels of -xylosidases activities induction. In addition, the application of an experimental design with this microorganism to induce -xylosidases has not been reported until the present work. The optimal conditions for the crude enzyme extract were pH 5.5 and 60 °C showing better thermostability at 55 °C. The saccharification ability of -xylosidase in the presence of hemicellulose obtained from corn straw raw and xylan from beechwood substrates showed a xylo-oligosaccharide to xylose conversion yield of 80 and 50%, respectively, at 50 °C. Our data strongly indicated that the -xylosidases activities was not subjected to the effects of potential enzyme inhibitors often produced during fermentation process. These data suggest the application of this enzyme studied for saccharification of hemicellulose, an abundant residue in the American continents, thus providing an interesting alternative for future tests for energy production.(AU)

Thermotolerant and mesophylic fungi from sugarcane bagasse and their prospection for biomass-degrading enzyme production

Nineteen fungi and seven yeast strains were isolated from sugarcane bagasse piles from an alcohol plant located at Brazilian Cerrado and identified up to species level on the basis of the gene sequencing of 5.8S-ITS and 26S ribosomal DNA regions. Four species were identified: Kluyveromyces marxianus, Aspergillus niger, Aspergillus sydowii and Aspergillus fumigatus, and the isolates were screened for the production of key enzymes in the saccharification of lignocellulosic material. Among them, three strains were selected as good producers of hemicellulolitic enzymes: A. niger (SBCM3), A. sydowii (SBCM7) and A. fumigatus (SBC4). The best β-xylosidase producer was A. niger SBCM3 strain. This crude enzyme presented optimal activity at pH 3.5 and 55 °C (141 U/g). For β-glucosidase and xylanase the best producer was A. fumigatus SBC4 strain, whose enzymes presented maximum activity at 60 °C and pH 3.5 (54 U/g) and 4.0 (573 U/g), respectively. All these crude enzymes presented stability around pH 3.0–8.0 and up to 60 °C, which can be very useful in industrial processes that work at high temperatures and low pHs. These enzymes also exhibited moderate tolerance to ethanol and the sugars glucose and xylose. These similar characteristics among these fungal crude enzymes suggest that they can be used synergistically in cocktails in future studies of biomass conversion with potential application in several biotechnological sectors..(AU)

Production of bioethanol using agricultural waste: banana pseudo stem

India is amongst the largest banana (Musa acuminata) producing countries and thus banana pseudo stem is commonly available agricultural waste to be used as lignocellulosic substrate. Present study focuses on exploitation of banana pseudo stem as a source for bioethanol production from the sugars released due to different chemical and biological pretreatments. Two fungal strains Aspergillus ellipticus and Aspergillus fumigatus reported to be producing cellulolytic enzymes on sugarcane bagasse were used under co-culture fermentation on banana pseudo stem to degrade holocellulose and facilitate maximum release of reducing sugars. The hydrolysate obtained after alkali and microbial treatments was fermented by Saccharomyces cerevisiae NCIM 3570 to produce ethanol. Fermentation of cellulosic hydrolysate (4.1 g%) gave maximum ethanol (17.1 g/L) with yield (84%) and productivity (0.024 g%/h) after 72 h. Some critical aspects of fungal pretreatment for saccharification of cellulosic substrate using A. ellipticus and A. fumigatus for ethanol production by S. cerevisiae NCIM 3570 have been explored in this study. It was observed that pretreated banana pseudo stem can be economically utilized as a cheaper substrate for ethanol production.

Ethanol production from agricultural wastes using Sacchromyces cervisae

The main objective of this study was production of ethanol from three lignocellulosic biomasses like sugarcane bagasse, rice straw and wheat straw by Sacchromyces cervisae. All the three substrates were ground to powder form (2 mm) and pretreated with 3%H2O2 + 2% NaOH followed by steaming at 130 °C for 60 min. These substrates were hydrolyzed by commercial cellulase enzyme. The whole fermentation process was carried out in 500 mL Erlenmeyer flask under anaerobic conditions in submerged fermentation at 30 °C for three days of incubation period. FTIR analysis of the substrates indicated significant changes in the alteration of the structure occurred after pretreatment which leads to efficient saccharification. After pretreatment the substrates were hydrolyzed by commercial cellulase enzyme and maximum hydrolysis was observed in sugarcane bagasse (64%) followed by rice straw (40%) and wheat straw (34%). Among all these tested substrates, sugarcane bagasse (77 g/L) produced more ethanol as compared to rice straw (62 g/L) and wheat straw (44 g/L) using medium composition of (%) 0.25 (NH4)2SO4, 0.1 KH2PO4, 0.05 MgSO4, 0.25 Yeast extract by S. cervisae.

Bioprospecting thermophiles for cellulase production: a review

Most of the potential bioprospecting is currently related to the study of the extremophiles and their potential use in industrial processes. Recently microbial cellulases find applications in various industries and constitute a major group of industrial enzymes. Considerable amount of work has been done on microbial cellulases, especially with resurgence of interest in biomass ethanol production employing cellulases and use of cellulases in textile and paper industry. Most efficient method of lignocellulosic biomass hydrolysis is through enzymatic saccharification using cellulases. Significant information has also been gained about the physiology of thermophilic cellulases producers and process development for enzyme production and biomass saccharification. The review discusses the current knowledge on cellulase producing thermophilic microorganisms, their physiological adaptations and control of cellulase gene expression. It discusses the industrial applications of thermophilic cellulases, their cost of production and challenges in cellulase research especially in the area of improving process economics of enzyme production.

Alkali pretreated of wheat straw and its enzymatic hydrolysis

The efficiency of enzymatic hydrolysis of cellulose can be improved by various pretreatments of the substrate. In order to increase the efficiency of enzymatic saccharification of the wheat straw, we determined the effect of different pretreatments on the physical structure, chemical components and enzymatic saccharification of wheat straw. Our results showed that combination of grinding and sodium hydroxide (NaOH) treatment had high effect on the enzymatic hydrolysis of wheat straws. The optimal pretreatment condition was to grind the wheat straws into the sizes of 120 meshes followed by treatment with 1.0% NaOH for 1.5 h (121°C/15psi). Under this condition, the cellulose content of wheat straw was increased by 44.52%, while the content of hemicellulose and lignin was decreased by 44.15% and 42.52%, respectively. Scanning Electronic Microscopy and infrared spectrum analyses showed that significant changes occurred in the structure of wheat straws after pretreatment, which is favorable for the hydrolysis and saccharification. Cellulase produced by Penicillium waksmanii F10-2 was used to hydrolyze the pretreated wheat straw and the optimal condition was determined to be 30 h of enzymatic reaction under the temperature of 55°C, pH 5.5 and substrate concentration of 3%.

Concentração de enzimas amilolíticas na hidrólise do amido de gengibre

The use of disqualifies ginger rhizomes as raw material for fresh fruit market is a way to allow the increase of ginger production . The starch is not directly fermentable, which requires prior hydrolysis to obtain glucose. This study aimed to evaluate the effect of concentrations of -amylase and amyloglucosidase on the yield and sugar profile in the process of hydrolysis-saccharification of ginger. The process followed the central composite design for two factors, totaling 11 treatments. The results showed the effect of concentrations of -amylase (Termamyl 2X) and amyloglucosidase (AMG 300L) on the glucose content and amyloglucosidase concentration effect on the content of dextrin. The higher yields of hydrolysis of the ginger suspension were obtained under conditions of high concentration of -amylase and amyloglucosidase.

Uma possibilidade de incremento na cadeia produtiva do gengibre seria o uso de rizomas desclassificados para comercialização in natura, ou mesmo o resíduo da extração de óleos, como matérias-primas para a obtenção de bebidas destiladas. O amido não é diretamente fermentável, necessitando de uma hidrólise prévia de suas cadeias para a obtenção de glicose. Neste trabalho, objetivou-se avaliar o efeito das concentrações de amilases sobre o perfil de açúcares e rendimento no processo de hidrólise-sacarificação de gengibre. O processo seguiu o delineamento central composto rotacional para dois fatores, totalizando 11 tratamentos. Os resultados obtidos mostram o efeito das concentrações da -amilase (Termamyl 2X) e da amiloglucosidase (AMG 300L) sobre o teor de glicose do hidrolisado e efeito da concentração de amiloglucosidase sobre o teor de dextrina. Os maiores rendimentos de hidrólise da suspensão de gengibre foram obtidos nas condições de elevada concentração de -amilase e amiloglucosidase.

Concentração de enzimas amilolíticas na hidrólise do amido de gengibre

The use of disqualifies ginger rhizomes as raw material for fresh fruit market is a way to allow the increase of ginger production . The starch is not directly fermentable, which requires prior hydrolysis to obtain glucose. This study aimed to evaluate the effect of concentrations of -amylase and amyloglucosidase on the yield and sugar profile in the process of hydrolysis-saccharification of ginger. The process followed the central composite design for two factors, totaling 11 treatments. The results showed the effect of concentrations of -amylase (Termamyl 2X) and amyloglucosidase (AMG 300L) on the glucose content and amyloglucosidase concentration effect on the content of dextrin. The higher yields of hydrolysis of the ginger suspension were obtained under conditions of high concentration of -amylase and amyloglucosidase.

Uma possibilidade de incremento na cadeia produtiva do gengibre seria o uso de rizomas desclassificados para comercialização in natura, ou mesmo o resíduo da extração de óleos, como matérias-primas para a obtenção de bebidas destiladas. O amido não é diretamente fermentável, necessitando de uma hidrólise prévia de suas cadeias para a obtenção de glicose. Neste trabalho, objetivou-se avaliar o efeito das concentrações de amilases sobre o perfil de açúcares e rendimento no processo de hidrólise-sacarificação de gengibre. O processo seguiu o delineamento central composto rotacional para dois fatores, totalizando 11 tratamentos. Os resultados obtidos mostram o efeito das concentrações da -amilase (Termamyl 2X) e da amiloglucosidase (AMG 300L) sobre o teor de glicose do hidrolisado e efeito da concentração de amiloglucosidase sobre o teor de dextrina. Os maiores rendimentos de hidrólise da suspensão de gengibre foram obtidos nas condições de elevada concentração de -amilase e amiloglucosidase.

Concentração de enzimas amilolíticas na hidrólise do amido de gengibre

The use of disqualifies ginger rhizomes as raw material for fresh fruit market is a way to allow the increase of ginger production . The starch is not directly fermentable, which requires prior hydrolysis to obtain glucose. This study aimed to evaluate the effect of concentrations of -amylase and amyloglucosidase on the yield and sugar profile in the process of hydrolysis-saccharification of ginger. The process followed the central composite design for two factors, totaling 11 treatments. The results showed the effect of concentrations of -amylase (Termamyl 2X) and amyloglucosidase (AMG 300L) on the glucose content and amyloglucosidase concentration effect on the content of dextrin. The higher yields of hydrolysis of the ginger suspension were obtained under conditions of high concentration of -amylase and amyloglucosidase.

Uma possibilidade de incremento na cadeia produtiva do gengibre seria o uso de rizomas desclassificados para comercialização in natura, ou mesmo o resíduo da extração de óleos, como matérias-primas para a obtenção de bebidas destiladas. O amido não é diretamente fermentável, necessitando de uma hidrólise prévia de suas cadeias para a obtenção de glicose. Neste trabalho, objetivou-se avaliar o efeito das concentrações de amilases sobre o perfil de açúcares e rendimento no processo de hidrólise-sacarificação de gengibre. O processo seguiu o delineamento central composto rotacional para dois fatores, totalizando 11 tratamentos. Os resultados obtidos mostram o efeito das concentrações da -amilase (Termamyl 2X) e da amiloglucosidase (AMG 300L) sobre o teor de glicose do hidrolisado e efeito da concentração de amiloglucosidase sobre o teor de dextrina. Os maiores rendimentos de hidrólise da suspensão de gengibre foram obtidos nas condições de elevada concentração de -amilase e amiloglucosidase.

Estudo energético da produção de biocombustível a partir do milho

This research analyzed the energetic consumption of etanol from the corn crops (Zea mays L.). The field surveys were carried out in the Midle Paranapanema River Region, São Paulo state, Brazil, in the period from January to December 2007. The energy consumption on stage of production and industrial processing of grain were evaluated. It was verified that the total energetic cost of the crop production corresponded to 15,633.7MJ ha-1, and the most onerous item was the "inputs" (77.5%). In the industrial step, the energetic consumption was equivalent to 3,882.2MJ t-1. The operations of "hydrolysis, saccharification and treatment of the broth" represented 50.2% of the total energetic expenditure. It was also observed an energetic cost of 7.9MJ L-1 in relation to the etanol produced in the main crop production operations, and 11.8MJ L-1 in the industrial processing. The energy balance of crop production and industrialization was of 1.2MJ.

Objetivou-se analisar energeticamente a produção de etanol a partir do milho (Zea mays L.). As pesquisas de campo foram realizadas na região paulista do Médio Paranapanema, São Paulo (SP), no período de janeiro a dezembro de 2007. Avaliou-se o consumo energético referente às fases de produção e processamento industrial do grão. O custo energético total da produção agrícola correspondeu a 15.633,7MJ ha-1, e o item mais oneroso foi o de "insumos" (77,5%). Nas etapas industriais, o consumo energético foi equivalente a 3.882,2MJ t-1. As operações de "hidrólise, sacarificação e tratamento do caldo" representaram 50,2% do dispêndio energético total. O custo energético do milho foi de 7,9MJ L-1 nas operações agronômicas de produção e 11,8MJ L-1 nas etapas de processamento industrial. O balanço energético do cultivo e da industrialização foi de 1,2MJ.

Estudo energético da produção de biocombustível a partir do milho

This research analyzed the energetic consumption of etanol from the corn crops (Zea mays L.). The field surveys were carried out in the Midle Paranapanema River Region, São Paulo state, Brazil, in the period from January to December 2007. The energy consumption on stage of production and industrial processing of grain were evaluated. It was verified that the total energetic cost of the crop production corresponded to 15,633.7MJ ha-1, and the most onerous item was the "inputs" (77.5%). In the industrial step, the energetic consumption was equivalent to 3,882.2MJ t-1. The operations of "hydrolysis, saccharification and treatment of the broth" represented 50.2% of the total energetic expenditure. It was also observed an energetic cost of 7.9MJ L-1 in relation to the etanol produced in the main crop production operations, and 11.8MJ L-1 in the industrial processing. The energy balance of crop production and industrialization was of 1.2MJ.

Objetivou-se analisar energeticamente a produção de etanol a partir do milho (Zea mays L.). As pesquisas de campo foram realizadas na região paulista do Médio Paranapanema, São Paulo (SP), no período de janeiro a dezembro de 2007. Avaliou-se o consumo energético referente às fases de produção e processamento industrial do grão. O custo energético total da produção agrícola correspondeu a 15.633,7MJ ha-1, e o item mais oneroso foi o de "insumos" (77,5%). Nas etapas industriais, o consumo energético foi equivalente a 3.882,2MJ t-1. As operações de "hidrólise, sacarificação e tratamento do caldo" representaram 50,2% do dispêndio energético total. O custo energético do milho foi de 7,9MJ L-1 nas operações agronômicas de produção e 11,8MJ L-1 nas etapas de processamento industrial. O balanço energético do cultivo e da industrialização foi de 1,2MJ.

Estudo energético da produção de biocombustível a partir do milho

This research analyzed the energetic consumption of etanol from the corn crops (Zea mays L.). The field surveys were carried out in the Midle Paranapanema River Region, São Paulo state, Brazil, in the period from January to December 2007. The energy consumption on stage of production and industrial processing of grain were evaluated. It was verified that the total energetic cost of the crop production corresponded to 15,633.7MJ ha-1, and the most onerous item was the "inputs" (77.5%). In the industrial step, the energetic consumption was equivalent to 3,882.2MJ t-1. The operations of "hydrolysis, saccharification and treatment of the broth" represented 50.2% of the total energetic expenditure. It was also observed an energetic cost of 7.9MJ L-1 in relation to the etanol produced in the main crop production operations, and 11.8MJ L-1 in the industrial processing. The energy balance of crop production and industrialization was of 1.2MJ.

Objetivou-se analisar energeticamente a produção de etanol a partir do milho (Zea mays L.). As pesquisas de campo foram realizadas na região paulista do Médio Paranapanema, São Paulo (SP), no período de janeiro a dezembro de 2007. Avaliou-se o consumo energético referente às fases de produção e processamento industrial do grão. O custo energético total da produção agrícola correspondeu a 15.633,7MJ ha-1, e o item mais oneroso foi o de "insumos" (77,5%). Nas etapas industriais, o consumo energético foi equivalente a 3.882,2MJ t-1. As operações de "hidrólise, sacarificação e tratamento do caldo" representaram 50,2% do dispêndio energético total. O custo energético do milho foi de 7,9MJ L-1 nas operações agronômicas de produção e 11,8MJ L-1 nas etapas de processamento industrial. O balanço energético do cultivo e da industrialização foi de 1,2MJ.

Avaliação energética da produção de etanol utilizando como matéria-prima a cana-de-açúcar

This research analyzed the energetic consumption of the etanol, using as raw-material sugar cane. The searches were carried out from the field in the Midle Paranapanema River Region, Sao Paulo state, in the period from January to December 2007. The referring energy consumption from the crop production phase and industrial processing were evaluated. It was verified that the total energetic cost of the crop production corresponded to 14370.9MJ ha-1, and the most onerous item was the "inputs" (50.4%). In the industrial step, the energetic consumption was equivalent to 1,641.56MJ t-1. The 'hydrolysis, saccharification and broth treatment operations' represented 71.72% of the total energetic expenditure. It was also observed an energetic cost of 2.0MJ L-1 in relation to the etanol produced in the main crop production operations, and 19.4MJ L-1 in the industrial processing. The energy efficiency observed in the crop production and industrialization were of 1.1.

Analisou-se energeticamente a produção de etanol originária da cana-de-açúcar. As pesquisas de campo foram realizadas na região paulista do Médio Paranapanema, no período de janeiro a dezembro de 2007, avaliando-se o consumo energético referente às fases de produção e processamento industrial. Verificou-se que o custo energético total da produção agrícola correspondeu a 14.370,9MJ ha-1, e o item mais oneroso foi o de "insumos" (50,4%). Nas etapas industriais, o consumo energético foi equivalente a 1.641,6MJ t-1. As operações de "hidrólise, sacarificação e tratamento do caldo" representaram 71,7% do dispêndio energético total. Na cana-de-açúcar, observou-se um custo energético de 2,0MJ L-1 em relação ao etanol produzido nas principais operações agronômicas de produção e 19,4MJ L-1 nas etapas de processamento industrial. A eficiência energética no cultivo e na industrialização foi de 1,1.

Avaliação energética da produção de etanol utilizando como matéria-prima a cana-de-açúcar

This research analyzed the energetic consumption of the etanol, using as raw-material sugar cane. The searches were carried out from the field in the Midle Paranapanema River Region, Sao Paulo state, in the period from January to December 2007. The referring energy consumption from the crop production phase and industrial processing were evaluated. It was verified that the total energetic cost of the crop production corresponded to 14370.9MJ ha-1, and the most onerous item was the "inputs" (50.4%). In the industrial step, the energetic consumption was equivalent to 1,641.56MJ t-1. The 'hydrolysis, saccharification and broth treatment operations' represented 71.72% of the total energetic expenditure. It was also observed an energetic cost of 2.0MJ L-1 in relation to the etanol produced in the main crop production operations, and 19.4MJ L-1 in the industrial processing. The energy efficiency observed in the crop production and industrialization were of 1.1.

Analisou-se energeticamente a produção de etanol originária da cana-de-açúcar. As pesquisas de campo foram realizadas na região paulista do Médio Paranapanema, no período de janeiro a dezembro de 2007, avaliando-se o consumo energético referente às fases de produção e processamento industrial. Verificou-se que o custo energético total da produção agrícola correspondeu a 14.370,9MJ ha-1, e o item mais oneroso foi o de "insumos" (50,4%). Nas etapas industriais, o consumo energético foi equivalente a 1.641,6MJ t-1. As operações de "hidrólise, sacarificação e tratamento do caldo" representaram 71,7% do dispêndio energético total. Na cana-de-açúcar, observou-se um custo energético de 2,0MJ L-1 em relação ao etanol produzido nas principais operações agronômicas de produção e 19,4MJ L-1 nas etapas de processamento industrial. A eficiência energética no cultivo e na industrialização foi de 1,1.

Avaliação energética da produção de etanol utilizando como matéria-prima a cana-de-açúcar

This research analyzed the energetic consumption of the etanol, using as raw-material sugar cane. The searches were carried out from the field in the Midle Paranapanema River Region, Sao Paulo state, in the period from January to December 2007. The referring energy consumption from the crop production phase and industrial processing were evaluated. It was verified that the total energetic cost of the crop production corresponded to 14370.9MJ ha-1, and the most onerous item was the "inputs" (50.4%). In the industrial step, the energetic consumption was equivalent to 1,641.56MJ t-1. The 'hydrolysis, saccharification and broth treatment operations' represented 71.72% of the total energetic expenditure. It was also observed an energetic cost of 2.0MJ L-1 in relation to the etanol produced in the main crop production operations, and 19.4MJ L-1 in the industrial processing. The energy efficiency observed in the crop production and industrialization were of 1.1.

Analisou-se energeticamente a produção de etanol originária da cana-de-açúcar. As pesquisas de campo foram realizadas na região paulista do Médio Paranapanema, no período de janeiro a dezembro de 2007, avaliando-se o consumo energético referente às fases de produção e processamento industrial. Verificou-se que o custo energético total da produção agrícola correspondeu a 14.370,9MJ ha-1, e o item mais oneroso foi o de "insumos" (50,4%). Nas etapas industriais, o consumo energético foi equivalente a 1.641,6MJ t-1. As operações de "hidrólise, sacarificação e tratamento do caldo" representaram 71,7% do dispêndio energético total. Na cana-de-açúcar, observou-se um custo energético de 2,0MJ L-1 em relação ao etanol produzido nas principais operações agronômicas de produção e 19,4MJ L-1 nas etapas de processamento industrial. A eficiência energética no cultivo e na industrialização foi de 1,1.

Xylanase production with xylan rich lignocellulosic wastes by a local soil isolate of Trichoderma viride

In the present study, cultural and nutritional conditions for enhanced production of xylanase by a local soil isolate of Trichoderma viride, using various lignocellulosic substrates in submerged culture fermentation have been optimized. Of the lignocellulosics used, maize straw was the best inducer followed by jowar straw for xylanase production. The highest activity achieved was between 14 to 17 days of fermentation. A continuous increase in xylanase production was observed with increasing level of lignocellulosics in the medium and highest activity was observed with maize straw at 5% level. Xylanase production with higher levels of lignocellulosics (3 to 5%) of maize, jowar and barseem was found to be higher as compared to that with commercial xylan as carbon source. Sodium nitrate was the best nitrogen source among the six sources used. Maximum xylanase production was achieved with initial medium pH of 3.5-4.0 and incubation temperature of 25ºC.The enzyme preparation was effective in bringing about saccharification of different lignocellulosics. The xylanase production could be further improved by using alkali treated straw as carbon source.

Neste estudo, otimizou-se as condições culturais e nutricionais para produção aumentada de xilanase por uma cepa local de Trichoderma viride isolada de solo, empregando-se vários substratos lignocelulósicos, em fermentação submersa. Entre os substratos utilizados, o melhor indutor de produção de xilanase foi palha de milho, seguido de palha de sorgo. A atividade mais alta foi obtida entre 14 e 17 dias de fermentação. Com palha de milho observou-se um aumento contínuo na produção de xilanase com o aumento da concentração dos substratos lignocelulósicos no meio, sendo que a melhor atividade foi obtida com 5% de palha de milho. A produção de xilanase com níveis mais altos de (3 a 5%) de milho, sorgo e forragem verde (barseem) foi mais levada do que com xilana comercial como fonte de carbono. Entre as fontes de nitrogênio testadas, a melhor foi nitrato de sódio. Produção máxima de xilanase foi obtida quando o pH inicial do meio foi 3,5 4,0 e a temperatura de incubação 25ºC. A enzima foi eficiente na sacarificação de diferentes substratos lignocelulósicos. A produção de xilanase poderia ser aumentada empregando-se álcali ao invés de palha tratada como fonte de carbono.