Lomustine for treatment of canine transmissible venereal tumor / Eficácia da lomustina no tratamento do tumor venéreo transmissível canino

Canine transmissible venereal tumor (TVTC) is a highly casuistic transmissible neoplasm in Brazil. Chemotherapy with vincristine sulfate is considered the treatment of choice, but the need for weekly applications and hematological monitoring, in addition to costs, are obstacles to owners' adhesion to the treatment. Lomustine is an alkylating class antineoplastic agent, and because it is administered orally, it is a more practical and less costly treatment option for the owners of animals with neoplasms sensitive to the drug. This study evaluated the therapeutic efficacy of lomustine in dogs affected by TVTC. Twelve dogs with cytopathological diagnosis of natural genital TVTC were selected. The dogs were submitted to the experimental protocol with lomustine administration at doses of 70 to 85 mg/m2 orally every 21 days, totaling a maximum of two administration cycles. The animals were reevaluated every 7 days until a maximum of +49 days after the first dose of lomustine, to monitor the regression of neoplastic lesions through measurements. Among the 12 dogs submitted to the lomustine protocol, 8/12 achieved complete remission of the neoplasm and were considered cured (66.6%), 1/12 had partial response to treatment (8.33%) and 3/12 had stable disease (25%). Important adverse effects such as severe neutrophilic leukopenia were detected in 3/12 dogs (25%). The clinical study indicated that lomustine may be a treatment option for TVTC.

O tumor venéreo transmissível canino (TVTC) é uma neoplasia transmissível de elevada casuística no Brasil. A quimioterapia com sulfato de vincristina é considerada o tratamento de escolha, mas a necessidade de aplicações semanais e acompanhamento hematológico, além dos custos, são obstáculos à adesão dos proprietários ao tratamento. A lomustina é um antineoplásico da classe dos agentes alquilantes e, por ser administrado por via oral, representa um opção de tratamento mais prática e menos onerosa para os proprietários de animais com neoplasias. O objetivo deste estudo foi avaliar a eficácia terapêutica da lomustina em cães acometidos por TVTC. Foram selecionados 12 cães com diagnóstico citopatológico de TVTC genital de ocorrência natural. Os cães foram submetidos ao protocolo experimental com administração de lomustina nas doses de 70 a 85 mg/m2 por via oral a cada 21 dias, totalizando no máximo dois ciclos de administração. Os animais foram reavaliados a cada sete dias até um máximo de +49 dias após a primeira dose de lomustina, para monitorar a regressão das lesões neoplásicas por meio de mensuração das lesões. Entre os 12 cães submetidos ao protocolo, 8/12 obtiveram remissão completa da neoplasia e foram considerados curados (66,6%), 1/12 tiveram resposta parcial ao tratamento (8,33%) e 3/12 tiveram doença estável (25%). Efeitos adversos importantes, como leucopenia neutrofílica grave, foram detectados em 3/12 cães (25%). O estudo clínico indicou que a lomustina pode ser uma opção de tratamento para TVTC.

Epidemiological, clinical, and therapeutic aspects of canine transmissible venereal tumor in Rio de Janeiro, Brazil (2015-2020) / Aspectos epidemiológicos, clínicos e terapêuticos do tumor venéreo transmissível canino no Rio de Janeiro, Brasil (2015-2020)

Canine transmissible venereal tumors (TVT) have a high incidence in Brazil. This is partly due to the large population of stray dogs and the ineffectiveness of epidemiological control programs. This study aimed to describe the epidemiological data, clinical manifestations, and treatments used in dogs affected by TVT. Data were retrospectively collected from the 2015-2020 records of the Veterinary Hospital of the Federal Rural University of Rio de Janeiro. A total of 252 dogs were diagnosed with TVT during the study period. Of these, 81.3% were mixed-breed, 50.4% were males, and 88.9% were young or adult animals. The genital region only was affected in 77.3% of cases. Exclusively extragenital lesions were observed in 22.6% of cases. Among the animals seen, 40.1% received no treatment. Of those treated, 99.3% underwent a vincristine sulfate protocol, and in 77.2%, the treatment resulted in total remission of the neoplasm after 4 to 6 chemotherapy sessions. It was concluded that TVT is a neoplasm most often seen in mixed-breed dogs and located in the genital region, with hemorrhagic secretion being the main clinical sign reported by owners. Vincristine sulfate is currently the most used therapy, with high efficacy. However, despite the good prognosis, there was a high rate of non-adherence or abandonment of treatment, and this is an important factor to be considered and addressed by veterinarians.

O tumor venéreo transmissível (TVT) apresenta elevada incidência no Brasil, relacionada a elevada população de caninos errantes e a ineficácia dos programas de controle epidemiológicos. O objetivo do estudo foi descrever dados epidemiológicos, manifestação clínica e o tratamento empregado em cães acometidos pelo TVT no Hospital Veterinário da Universidade Federal Rural do Rio de Janeiro entre os anos de 2015 e 2020. Foram diagnosticados 252 cães com TVT durante o período do estudo, sendo 81,3% cães sem raça definida, 50,4% machos e 49,6% fêmeas, e com 88,9% animais jovens ou adultos. A região genital foi acometida em 77,3% dos casos. Lesões exclusivamente extragenitais foram observadas em 22,6% dos casos. Quanto ao tratamento, 40,1% dos cães não receberam tratamento. Entre os animais tratados, 99,3% utilizaram protocolo com sulfato de vincristina e em 77,2% o tratamento resultou em remissão total da neoplasia, com a realização de 4 a 6 sessões do quimioterápico. Conclui-se que o TVT é uma neoplasia frequentemente relacionada a cães sem raça definida, localizados na região genital, com secreção hemorrágica sendo o principal sinal clínico reportado pelos proprietários. O sulfato de vincristina é a terapia mais empregada, com alta eficácia. Entretanto, apesar de ser uma neoplasia com bom prognóstico, o alto índice de não adesão ou abandono ao tratamento representa um importante fator a ser considerado e trabalhado pelos médicos veterinários.

Could dysregulation of RASSF1 expression be a mechanism of tumorigenesis in CTVT? / A desregulação da expressão de RASSF1 pode ser um mecanismo da tumorigênese em TVTC?

Canine transmissible venereal tumor (CTVT) is the oldest known somatic cell lineage. It is a transmissible cancer that propagates naturally in dogs and reportedly contains gene mutations. RASSF1 participates in DNA damage repair, and its downregulation, results in tumor progression. Hence, RASSF1 is a tumor suppressor gene. Its expression was quantified in tumors from seventeen animals and three cell cultures derived from tumors. In general, RASSF1 was underexpressed in 65%, and absent in 35% of tumor samples. Cells from tumor tissue cultures showed decreased expression of RASSF1 in 67% and elevated expression in 33% of samples tested. The tumor tissues showed significantly lower levels of RASSF1 expression compared to cultured cells. Previously we reported that both the tumor microenvironment and the host immune system appear to influence the tumorigenesis and stage of CTVT. This is the first article to demonstrate the expression of RASSF1 in CTVT. Decreased RASSF1 possibly helps tumor progression.

O tumor venéreo transmissível canino (TVTC) é a linhagem de células somáticas mais antiga conhecida. É um câncer transmissível que se propaga naturalmente em cães e mutações genéticas já foram relatadas. O gene RASSF1 atua no reparo de danos ao DNA e presume-se que, quando suprimido ou com expressão gênica reduzida, o TVTC tende a progredir. A expressão do gene supressor de tumor, como RASSF1, foi quantificada em tecidos de dezessete animais e três culturas de células de tecidos tumorais. Em geral, o gene RASSF1 apresentou prevalência de subexpressão (65%) e ausência em 35% dos demais tecidos analisados. Células isoladas de culturas de tecidos tumorais também demonstraram 67% com expressão diminuída e 33% com expressão elevada, com diferença significativa entre os níveis de expressão gênica em amostras de tecido quando comparadas às culturas de células, com tecidos apresentando níveis mais baixos de expressão gênica em comparação com células. Anteriormente, relatamos que tanto o microambiente tumoral quanto o sistema imunológico do hospedeiro parecem influenciar a tumorigênese e o estágio do TVTC. Este é o primeiro artigo a demonstrar a expressão de RASSF1 no TVTC, possivelmente alterando sua tumorigênese e auxiliando no aumento da progressão tumoral.

Canine transmissible venereal tumor with spontaneous remission: case study with emphasis on clinical and cytopathological exams to monitor tumor evolution / Tumor venéreo transmissível canino com remissão espontânea: estudo de caso com ênfase aos exames clínico e citopatológico para monitoramento da evolução tumoral

The canine transmissible venereal tumor (TVTC) is a neoplasm transmitted mainly through copulation and with a high incidence in stray dogs in Brazil. In the process of tumor evolution of TVTC, the progression, stationary and regression phases are recognized. The host immunity is related to the disease's biological behavior, however, spontaneous regression observation in cases of naturally occurring TVTC is uncommon. A canine patient was attended, after beeing rescued from the street, due to an ulcerated mass in the external genitália and tick infestation. Cytopathological examination, which diagnosed TVTC, and laboratory tests that showed mild anemia and severe thrombocytopenia were performed. In view of the impossibility of carrying out other exams, it was made the presumptive diagnosis of canine monocytic ehrlichiosis (CME), and treatment was instituted. During follow-up it was observed quick improvement in clinical signs and laboratory changes, as well as a reduction in tumor mass. A new cytopathological evaluation was carried out, and was verified increase in mature lymphocytes and plasmocytes in the midst of the tumor cells, finding compatible with the stationary phase of the disease. From that moment on, it was decided to perform only clinical and cytopathological follow-up. In the following evaluations, continuous clinical remission and cytopathological findings compatible with those described in the regression phase were observed, until its complete remission. It is considered that the improvement in the general health of the patient after the treatment of CME is related to the spontaneous regression of TVTC, and that simultaneous performance of serial clinical and cytopathological exams may be feasible and useful for monitoring the stages of evolution of TVTC.

O tumor venéreo transmissível canino (TVTC) é uma neoplasia transmitida principalmente através da cópula, com elevada incidência em cães errantes no Brasil. No processo de evolução tumoral do TVTC, são reconhecidas as fases de progressão, estacionária e de regressão. O estado imunológico do hospedeiro está relacionado ao comportamento biológico da doença, contudo, a observação de regressão espontânea em casos de TVTC de ocorrência natural é incomum. Foi atendida uma paciente canina, resgatada da rua, por apresentar massa ulcerada na genitália externa e infestação por carrapatos. Foram realizados exame citopatológico, que diagnosticou TVTC, e exames laboratoriais que evidenciaram anemia discreta e grave trombocitopenia. Com isso e diante da impossibilidade de realizar outros exames, foi também estabelecido o diagnóstico presuntivo de erlichiose monocítica canina (EMC) e instituído tratamento para a hemoparasitose. Durante o acompanhamento, foi observada rápida melhora dos sinais clínicos e das alterações laboratoriais, bem como a redução espontânea da massa tumoral. Em sequência, foi realizada nova avaliação citopatológica do TVTC e verificado o aumento quantitativo de linfócitos maduros e plasmócitos, em meio as células tumorais, achado compatível com a fase estacionária da doença. A partir desse momento, optou-se por realizar apenas acompanhamento clínico e avaliação citopatológica da neoplasia. Foram observados contínua remissão clínica e achados microscópicos compatíveis com a fase de regressão do tumor, até sua remissão completa. Pondera-se que a melhora na saúde geral da paciente após o tratamento da EMC esteja relacionada à regressão espontânea do TVTC, e que realização simultânea de exames clínico e citopatológico seriados pode ser viável e útil ao acompanhamento das fases de evolução do TVTC.

Vincristine sulfate treatment influence on kidney function of female dogs with transmissible venereal tumor / Influência do tratamento com sulfato de vincristina na função renal de cadela com tumor transmissível venéreo

Chemotherapy agents have some undesirable and non-selective cytostatic effects. Considering that kidneys are vulnerable to drug-induced toxicity, this study evaluated renal injury caused by vincristine sulfate (VS) in 12 female dogs diagnosed with transmissible venereal tumor (TVT). The animals were treated with VS (0.025 mg/kg IV) every 7 days for 4 weeks. During treatment, the animals were subjected to clinical examination, blood count, serum measurement of symmetric dimethylarginine (SDMA), blood urea nitrogen (BUN), creatinine, alanine aminotransferase, and alkaline phosphatase. In addition, urinalysis and urinary gamma-glutamyl transferase (GGT) measurements were performed. All parameters were determined three times: before beginning the treatment (T0), after 14 days (T1), and after 28 days (T2). During the study period, there were no changes in serum urea or creatinine levels, urine specific gravity, or persistent proteinuria. Furthermore, urinary GGT measurement did not indicate tubular lesions, and consistent elevation of SDMA was found in only one patient above the reference range. The results showed that weekly therapy with VS as a single agent for 28 days does not induce renal injury in most cases.(AU)

Os agentes quimioterápicos possuem efeitos citostáticos indesejáveis e não seletivos. Considerando a vulnerabilidade renal à toxicidade induzida por drogas, este estudo avaliou a lesão renal causada pelo sulfato de vincristina (VS) em 12 cadelas com diagnóstico de tumor venéreo transmissível (TVT). Os animais foram tratados com VS (0,025 mg / kg IV) a cada sete dias, durante quatro semanas. No transcurso do tratamento, os animais foram submetidos a exame clínico, hemograma, dosagem sérica de dimetilarginina simétrica (SDMA), nitrogênio ureico sanguíneo (BUN), creatinina, alanina aminotransferase e fosfatase alcalina. Além disso, foram realizadas análises de urina e medições de gama-glutamil transferase (GGT) urinária. Todos os parâmetros foram mensurados em três tempos, antes do início do tratamento (T0), aos 14 dias (T1) e aos 28 dias (T2). Durante o período do estudo, não houve alterações nas concentrações de ureia ou creatinina séricas, na gravidade específica da urina ou proteinúria persistente. Além disso, a medição de GGT urinária não indicou lesões tubulares, e elevação consistente de SDMA foi encontrada em apenas um paciente acima do intervalo de referência. Os resultados mostraram que a terapia semanal com VS como agente único por 28 dias não induz lesão renal na maioria dos casos.(AU)

Tumor venéreo transmissível canino em laringe com metástase pulmonar / Canine transmissible venereal tumor in the larynx with pulmonary metastasis

Background: Canine transmissible venereal tumor (TVT) is one of the most common canine neoplasms in Brazil. Lesions develop mainly in the genital tissue and less frequently in other areas, in the extragenital form. Metastasis is rare, and the disease progression depends on the patient’s immune status. The treatment of choice is chemotherapy with vincristine sulfate, considered an effective and safe modality. The present work describes the first case of extragenital TVT in the larynx with pulmonary metastasis at the time of diagnosis. Case: An approximately 4-month-old mixed-breed bitch was attended manifesting acute clinical respiratory signs. The patient had been rescued from the street 2 months beforehand. Physical examination revealed pale mucous membranes and crackling pulmonary auscultation. Thoracic radiography was performed in 3 projections, showing multiple nodules in the lung parenchyma, followed by cytopathological examination using fine needle puncture guided by thoracic ultrasound, confirming the diagnosis of TVT. Considering the cytological finding and the absence of primary tumor lesions in the genital, oral, cutaneous or nasal regions, endoscopy was performed in order to locate a possible primary focus. The exam revealed a small erythematous tumor with smooth and regular surface, measuring approximately 2.5 cm, located in the larynx, between the arytenoid cartilages. Chemotherapy was performed with vincristine sulfate with radiographic follow-up throughout the treatment. Full remission of the pulmonary and laryngeal nodules was achieved at the end of the protocol. The animal remained in complete remission for a period of 2 years. Discussion: TVT is more frequently observed in animals of active reproductive age, and rarely in animals under 1 year of age, as in the case...

Tumor venéreo transmissível (TVT) nasal em cães / Nasal transmissible venereal tumor (TVT) in dogs

Background: Transmissible venereal tumors (TVT) are naturally occurring neoplasms that can be transmitted throughcopulation or cell transplantation. It is a disease that affects canines, has no preference for sex or breed, and generallynoticed in the external genital apparatus. Extragenital occurrence may eventually be seen; however, nasal involvementhas been described in only a few reports of studies conducted in Brazil. Therefore, the objective of this study is to report3 cases of nasal TVT in dogs who were treated in 2 municipalities in the mountainous region of Santa Catarina, Brazil.Cases: This case report includes 3 male mixed-breed canines of age 3-13. Only 1 of the animals was castrated. As per themedical history, some points, such as an enlarged nasal region, sneezing, nasal discharge, and hoarseness, reported by thedogs’ respective owners were similar among all the dogs. Likewise, nosebleed was observed on physical examination inall the cases. The result of cytological examination was inconclusive only in 1 case. Rhinoscopy, incisional biopsy, andhistopathological examination were then performed for achieving a definitive diagnosis. In the 2 cases wherein cytologygave conclusive results, the cytological smears showed changes suggestive of TVT, such as cells with eccentric nuclei andlittle cytoplasm, which had vacuoles inside them. In 2 cases, radiographic examinations of the skull were also performed.The images showed changes in bone radiopacity, conformation of trabeculae with areas of bone lysis and cell proliferation,and irregularity in the contour of the nasal bone. After TVT diagnosis was confirmed, chemotherapy was initiated usingvincristine at a dose of 0.75 mg/m2 for 2 cases and 0.025 mg/kg for the remaining case...

Tumor venéreo transmissível (TVT) nasal em cães / Nasal transmissible venereal tumor (TVT) in dogs

Background: Transmissible venereal tumors (TVT) are naturally occurring neoplasms that can be transmitted throughcopulation or cell transplantation. It is a disease that affects canines, has no preference for sex or breed, and generallynoticed in the external genital apparatus. Extragenital occurrence may eventually be seen; however, nasal involvementhas been described in only a few reports of studies conducted in Brazil. Therefore, the objective of this study is to report3 cases of nasal TVT in dogs who were treated in 2 municipalities in the mountainous region of Santa Catarina, Brazil.Cases: This case report includes 3 male mixed-breed canines of age 3-13. Only 1 of the animals was castrated. As per themedical history, some points, such as an enlarged nasal region, sneezing, nasal discharge, and hoarseness, reported by thedogs respective owners were similar among all the dogs. Likewise, nosebleed was observed on physical examination inall the cases. The result of cytological examination was inconclusive only in 1 case. Rhinoscopy, incisional biopsy, andhistopathological examination were then performed for achieving a definitive diagnosis. In the 2 cases wherein cytologygave conclusive results, the cytological smears showed changes suggestive of TVT, such as cells with eccentric nuclei andlittle cytoplasm, which had vacuoles inside them. In 2 cases, radiographic examinations of the skull were also performed.The images showed changes in bone radiopacity, conformation of trabeculae with areas of bone lysis and cell proliferation,and irregularity in the contour of the nasal bone. After TVT diagnosis was confirmed, chemotherapy was initiated usingvincristine at a dose of 0.75 mg/m2 for 2 cases and 0.025 mg/kg for the remaining case...(AU)

Tumor Venéreo Transmissível (TVT) nasal com metástase óssea em um cão / Nasal transmissible venereal tumor (TVT) with bone metastasis in a dog

Background: Transmissible venereal tumor (TVT) is a highly contagious round cell neoplasm that affects dogs, and itis usually transmitted through coitus. The tumor is mainly located in the genital area; however, the neoplasm can also beextragenital, affecting the nose, mouth, and eyes, as well as the skin and superficial lymph nodes. Cytological examinationis the most commonly used method for definitive diagnosis due to its low cost and fast execution. Chemotherapy, radiotherapy, surgical resection, and other procedures such as cryosurgery are the possible treatment options. The objective ofthis report was to describe a case of extragenital TVT with nasal primary site and metastasis in the bone tissue in a dogtreated at a private veterinary hospital in the city of Belém, Pará, Brazil.Case: A 6-year-old male domiciliary Labrador Retriever dog, weighing 24.2 kg, received oncologic treatment in a privateveterinary hospital in the city of Belém, Pará, Brazil. The animal had a history of neoplastic disease, and he had undergoneTVT resection associated with chemotherapy treatment more than 3 years ago. The clinical examination revealed a volumeincrease in the periorbital region, left lateral ocular displacement, left nostril excessive epistaxis, recurrent sneezing, cough,and pain signs, and tumor metastasis was suspected. Complementary exams of oncological cytology, computed tomography(CT), hemogram, and serum biochemistry were requested for diagnosis and staging of the condition, and supportive therapywas prescribed. The cytological report showed a dense population of neoplastic round cells with characteristics of TVT. CTindicated the presence of a heterogeneous hypodense mass with irregular contours and partially defined limits, with slightuptake of the intravenously injected contrast medium that obliterated the nasal cavity, maxillary recess, nasopharyngealmeatus, frontal sinus, and sphenoid sinus on the left side...

Tumor Venéreo Transmissível (TVT) nasal com metástase óssea em um cão / Nasal transmissible venereal tumor (TVT) with bone metastasis in a dog

Background: Transmissible venereal tumor (TVT) is a highly contagious round cell neoplasm that affects dogs, and itis usually transmitted through coitus. The tumor is mainly located in the genital area; however, the neoplasm can also beextragenital, affecting the nose, mouth, and eyes, as well as the skin and superficial lymph nodes. Cytological examinationis the most commonly used method for definitive diagnosis due to its low cost and fast execution. Chemotherapy, radiotherapy, surgical resection, and other procedures such as cryosurgery are the possible treatment options. The objective ofthis report was to describe a case of extragenital TVT with nasal primary site and metastasis in the bone tissue in a dogtreated at a private veterinary hospital in the city of Belém, Pará, Brazil.Case: A 6-year-old male domiciliary Labrador Retriever dog, weighing 24.2 kg, received oncologic treatment in a privateveterinary hospital in the city of Belém, Pará, Brazil. The animal had a history of neoplastic disease, and he had undergoneTVT resection associated with chemotherapy treatment more than 3 years ago. The clinical examination revealed a volumeincrease in the periorbital region, left lateral ocular displacement, left nostril excessive epistaxis, recurrent sneezing, cough,and pain signs, and tumor metastasis was suspected. Complementary exams of oncological cytology, computed tomography(CT), hemogram, and serum biochemistry were requested for diagnosis and staging of the condition, and supportive therapywas prescribed. The cytological report showed a dense population of neoplastic round cells with characteristics of TVT. CTindicated the presence of a heterogeneous hypodense mass with irregular contours and partially defined limits, with slightuptake of the intravenously injected contrast medium that obliterated the nasal cavity, maxillary recess, nasopharyngealmeatus, frontal sinus, and sphenoid sinus on the left side...(AU)

Pulmonary metastasis of transmissible venereal tumour in a dog: a case report / Metastase pulmonar de tumor venéreo transmissível em um cão: relato de caso

The transmissible venereal tumour (TVT) is one of the most frequent neoplasias in dogs. This tumour has specific characteristics, and it is exclusively of canines. Its transmission occurs through viable neoplastic cell transplantation when in contact with mucosa or unhealthy skin and rarely metastasise. This paper aims to report a rare presentation of pulmonary metastasis of widespread transmissible venereal tumours in a Blue Heeler dog. The patient was cachectic, dyspnoeic, and dehydrated and had multiple skin and pharynx nodulations. The cytology of all cutaneous nodulations showed round vacuolated cells with large eccentric nuclei and loose chromatin, which is compatible with TVT’s microscopic characteristics. Owing to the clinical evolution and reserved prognosis, the patient was euthanized. Necroscopy revealed a mass in the right pulmonary caudal lobe. The mass showed the same histopathologic characteristic of the others: not encapsulated infiltrative neoplastic proliferation of round vacuolated cells. The atypical manifestation of cutaneous metastasis and mainly pulmonary metastasis, in this case, denote the importance of TVT inclusion as a differential in cutaneous neoplasia, even if they show distant organ metastasis. Therefore, it emphasised the importance of cytology and histology in the diagnosis of nodular affections.

O tumor venéreo transmissível (TVT) é uma das neoplasias mais frequentes em cães. Com características específi-cas, esse tumor é exclusivo da espécie canina. Sua transmição acontece através da transplantação de células neoplásicas viáveis quando em contato com mucosas ou pele não íntegra, raramente ocorrendo metástases. Este trabalho visa relatar a apresen-tação rara de metástase pulmonar de tumor venéreo transmissível generalizado em um cão Blue Heeler. O paciente apresen-tava-se caquético, dispneico, desidratado e com diversas nodulações cutâneas e em faringe. A citologia de todas as nodulações cutâneas evidenciaram células redondas vacuolizadas, com núcleo excêntrico grande e cromatina frouxa, compatível com as características microscópicas de TVT. Em decorrência da evolução clínica e prognóstico reservado, o paciente foi eutanasiado. No exame macroscópico foi evidenciado massa em lobo caudal do pulmão direito com as mesmas características histopatoló-gicas dos demais nódulos, proliferação neoplásica de células redondas vacuolizadas infiltrativas não encapsuladas. A manifes-tação atípica de metástases cutâneas e principalmente pulmonar neste caso denota a importância da inclusão do TVT como diferencial de neoplasias cutâneas mesmo com metástase em órgãos. Com isso ressalta-sea importância de exames de citopa-tologia e histopatologia no diagnóstico de afecções nodulares.

Pulmonary metastasis of transmissible venereal tumour in a dog: a case report / Metastase pulmonar de tumor venéreo transmissível em um cão: relato de caso

The transmissible venereal tumour (TVT) is one of the most frequent neoplasias in dogs. This tumour has specific characteristics, and it is exclusively of canines. Its transmission occurs through viable neoplastic cell transplantation when in contact with mucosa or unhealthy skin and rarely metastasise. This paper aims to report a rare presentation of pulmonary metastasis of widespread transmissible venereal tumours in a Blue Heeler dog. The patient was cachectic, dyspnoeic, and dehydrated and had multiple skin and pharynx nodulations. The cytology of all cutaneous nodulations showed round vacuolated cells with large eccentric nuclei and loose chromatin, which is compatible with TVTs microscopic characteristics. Owing to the clinical evolution and reserved prognosis, the patient was euthanized. Necroscopy revealed a mass in the right pulmonary caudal lobe. The mass showed the same histopathologic characteristic of the others: not encapsulated infiltrative neoplastic proliferation of round vacuolated cells. The atypical manifestation of cutaneous metastasis and mainly pulmonary metastasis, in this case, denote the importance of TVT inclusion as a differential in cutaneous neoplasia, even if they show distant organ metastasis. Therefore, it emphasised the importance of cytology and histology in the diagnosis of nodular affections.(AU)

O tumor venéreo transmissível (TVT) é uma das neoplasias mais frequentes em cães. Com características específi-cas, esse tumor é exclusivo da espécie canina. Sua transmição acontece através da transplantação de células neoplásicas viáveis quando em contato com mucosas ou pele não íntegra, raramente ocorrendo metástases. Este trabalho visa relatar a apresen-tação rara de metástase pulmonar de tumor venéreo transmissível generalizado em um cão Blue Heeler. O paciente apresen-tava-se caquético, dispneico, desidratado e com diversas nodulações cutâneas e em faringe. A citologia de todas as nodulações cutâneas evidenciaram células redondas vacuolizadas, com núcleo excêntrico grande e cromatina frouxa, compatível com as características microscópicas de TVT. Em decorrência da evolução clínica e prognóstico reservado, o paciente foi eutanasiado. No exame macroscópico foi evidenciado massa em lobo caudal do pulmão direito com as mesmas características histopatoló-gicas dos demais nódulos, proliferação neoplásica de células redondas vacuolizadas infiltrativas não encapsuladas. A manifes-tação atípica de metástases cutâneas e principalmente pulmonar neste caso denota a importância da inclusão do TVT como diferencial de neoplasias cutâneas mesmo com metástase em órgãos. Com isso ressalta-sea importância de exames de citopa-tologia e histopatologia no diagnóstico de afecções nodulares.(AU)

Comparison between hematological and biochemical changes caused by conventional and metronomic chemotherapies in the treatment of canine transmissible venereal tumor

ABSTRACT: This study aimed to report the hematological and biochemical changes caused by conventional and metronomic chemotherapies, using vincristine sulfate to treat canine Transmissible Venereal Tumor (TVT). Twelve dogs were selected, six of them for the group receiving conventional chemotherapy (G1), and six dogs for the group receiving metronomic chemotherapy (G2). The G1 group received vincristine sulfate once a week at the dose of 0.75mg/m² until the tumor had disappeared with treatment, and the G2 group was treated with vincristine sulfate three times a week at the dose of 0.25mg/m2 until the tumor had disappeared. Before and after chemotherapy treatment, hematological and biochemical blood tests were performed to evaluate the main alterations caused by both chemotherapeutic models. Dogs undergoing conventional chemotherapy had significant leukocyte changes (p 0.05), causing neutropenia and leukopenia. In dogs undergoing metronomic chemotherapy, leukocytes remained within the reference range. Half of the dogs in group G1 had normochromic, normocytic anemia. The only biochemical alteration observed was the increase of urea in group G2. Thus, metronomic chemotherapy for the treatment of TVT with vincristine sulfate proved to be an excellent method for treatment, with fewer adverse effects, especially in maintaining the leukogram of dogs within normal range and reducing the number of anemia in animals during treatment.

RESUMO: Esta pesquisa teve como objetivo relatar as alterações hematológicas e bioquímicas causadas pelo tratamento quimioterápico convencional e pela quimioterapia metronômica, utilizando-se sulfato de vincristina para o tratamento do tumor venéreo transmissível canino(TVTC). Foram selecionados 12 cães, sendo seis para o grupo que recebeu quimioterapia convencional (G1) e seis cães para o grupo que recebeu quimioterapia metronômica (G2). O grupo G1 recebeu sulfato de vincristina, uma vez por semana, na dose de 0,75mg/m2, até o desaparecimento do tumor e o grupo G2 foi tratado com sulfato de vincristina, três vezes por semana, na dose de 0,25mg/m2, até o desaparecimento do tumor. Antes e após o tratamento quimioterápico foram realizados exames hematológicos e bioquímicos sanguíneos para avaliação das principais alterações causadas pelos dois modelos quimioterápicos. Os cães submetidos à quimioterapia convencional tiveram alterações leucocitárias significativas (p 0,05), causando uma leucopenia por neutropenia enquanto nos cães, submetidos à quimioterapia metronômica, os leucócitos mantiveram-se dentro do intervalo de referência. A metade dos cães do grupo G1 tiveram uma anemia do tipo normocítica normocrômica. A única alteração bioquímica observada foi o aumento da ureia no grupo G2. Desta forma, a quimioterapia metronômica para o tratamento do TVT com sulfato de vincristina, demonstrou ser um excelente método para a cura do animal, com menores reduções de efeitos adversos, sobretudo, na manutenção do leucograma dos cães e na redução de animais com anemia.

Comparison between hematological and biochemical changes caused by conventional and metronomic chemotherapies in the treatment of canine transmissible venereal tumor / Comparação entre as alterações hematológicas e bioquímicas ocasionadas pela quimioterapia convencional e quimioterapia metronômica no tratamento do tumor venéreo transmissível canino

This study aimed to report the hematological and biochemical changes caused by conventional and metronomic chemotherapies, using vincristine sulfate to treat canine Transmissible Venereal Tumor (TVT). Twelve dogs were selected, six of them for the group receiving conventional chemotherapy (G1), and six dogs for the group receiving metronomic chemotherapy (G2). The G1 group received vincristine sulfate once a week at the dose of 0.75mg/m² until the tumor had disappeared with treatment, and the G2 group was treated with vincristine sulfate three times a week at the dose of 0.25mg/m2 until the tumor had disappeared. Before and after chemotherapy treatment, hematological and biochemical blood tests were performed to evaluate the main alterations caused by both chemotherapeutic models. Dogs undergoing conventional chemotherapy had significant leukocyte changes (p<0.05), causing neutropenia and leukopenia. In dogs undergoing metronomic chemotherapy, leukocytes remained within the reference range. Half of the dogs in group G1 had normochromic, normocytic anemia. The only biochemical alteration observed was the increase of urea in group G2. Thus, metronomic chemotherapy for the treatment of TVT with vincristine sulfate proved to be an excellent method for treatment, with fewer adverse effects, especially in maintaining the leukogram of dogs within normal range and reducing the number of anemia in animals during treatment.(AU)

Esta pesquisa teve como objetivo relatar as alterações hematológicas e bioquímicas causadas pelo tratamento quimioterápico convencional e pela quimioterapia metronômica, utilizando-se sulfato de vincristina para o tratamento do tumor venéreo transmissível canino(TVTC). Foram selecionados 12 cães, sendo seis para o grupo que recebeu quimioterapia convencional (G1) e seis cães para o grupo que recebeu quimioterapia metronômica (G2). O grupo G1 recebeu sulfato de vincristina, uma vez por semana, na dose de 0,75mg/m2, até o desaparecimento do tumor e o grupo G2 foi tratado com sulfato de vincristina, três vezes por semana, na dose de 0,25mg/m2, até o desaparecimento do tumor. Antes e após o tratamento quimioterápico foram realizados exames hematológicos e bioquímicos sanguíneos para avaliação das principais alterações causadas pelos dois modelos quimioterápicos. Os cães submetidos à quimioterapia convencional tiveram alterações leucocitárias significativas (p<0,05), causando uma leucopenia por neutropenia enquanto nos cães, submetidos à quimioterapia metronômica, os leucócitos mantiveram-se dentro do intervalo de referência. A metade dos cães do grupo G1 tiveram uma anemia do tipo normocítica normocrômica. A única alteração bioquímica observada foi o aumento da ureia no grupo G2. Desta forma, a quimioterapia metronômica para o tratamento do TVT com sulfato de vincristina, demonstrou ser um excelente método para a cura do animal, com menores reduções de efeitos adversos, sobretudo, na manutenção do leucograma dos cães e na redução de animais com anemia.(AU)

Comparison between hematological and biochemical changes caused by conventional and metronomic chemotherapies in the treatment of canine transmissible venereal tumor / Comparação entre as alterações hematológicas e bioquímicas ocasionadas pela quimioterapia convencional e quimioterapia metronômica no tratamento do tumor venéreo transmissível canino

This study aimed to report the hematological and biochemical changes caused by conventional and metronomic chemotherapies, using vincristine sulfate to treat canine Transmissible Venereal Tumor (TVT). Twelve dogs were selected, six of them for the group receiving conventional chemotherapy (G1), and six dogs for the group receiving metronomic chemotherapy (G2). The G1 group received vincristine sulfate once a week at the dose of 0.75mg/m² until the tumor had disappeared with treatment, and the G2 group was treated with vincristine sulfate three times a week at the dose of 0.25mg/m2 until the tumor had disappeared. Before and after chemotherapy treatment, hematological and biochemical blood tests were performed to evaluate the main alterations caused by both chemotherapeutic models. Dogs undergoing conventional chemotherapy had significant leukocyte changes (p<0.05), causing neutropenia and leukopenia. In dogs undergoing metronomic chemotherapy, leukocytes remained within the reference range. Half of the dogs in group G1 had normochromic, normocytic anemia. The only biochemical alteration observed was the increase of urea in group G2. Thus, metronomic chemotherapy for the treatment of TVT with vincristine sulfate proved to be an excellent method for treatment, with fewer adverse effects, especially in maintaining the leukogram of dogs within normal range and reducing the number of anemia in animals during treatment.(AU)

Esta pesquisa teve como objetivo relatar as alterações hematológicas e bioquímicas causadas pelo tratamento quimioterápico convencional e pela quimioterapia metronômica, utilizando-se sulfato de vincristina para o tratamento do tumor venéreo transmissível canino(TVTC). Foram selecionados 12 cães, sendo seis para o grupo que recebeu quimioterapia convencional (G1) e seis cães para o grupo que recebeu quimioterapia metronômica (G2). O grupo G1 recebeu sulfato de vincristina, uma vez por semana, na dose de 0,75mg/m2, até o desaparecimento do tumor e o grupo G2 foi tratado com sulfato de vincristina, três vezes por semana, na dose de 0,25mg/m2, até o desaparecimento do tumor. Antes e após o tratamento quimioterápico foram realizados exames hematológicos e bioquímicos sanguíneos para avaliação das principais alterações causadas pelos dois modelos quimioterápicos. Os cães submetidos à quimioterapia convencional tiveram alterações leucocitárias significativas (p<0,05), causando uma leucopenia por neutropenia enquanto nos cães, submetidos à quimioterapia metronômica, os leucócitos mantiveram-se dentro do intervalo de referência. A metade dos cães do grupo G1 tiveram uma anemia do tipo normocítica normocrômica. A única alteração bioquímica observada foi o aumento da ureia no grupo G2. Desta forma, a quimioterapia metronômica para o tratamento do TVT com sulfato de vincristina, demonstrou ser um excelente método para a cura do animal, com menores reduções de efeitos adversos, sobretudo, na manutenção do leucograma dos cães e na redução de animais com anemia.(AU)

Hepatozoonose canina associada a quimioterapia para linfossarcoma de Sticker em Santa Catarina, Brasil / Canine hepatozoonosis associated with chemotherapy for Sticker's Lymphosarcoma in Santa Catarina, Brazil

A hepatozoonose canina, causada pelo protozoário Hepatozoon spp, é uma enfermidade que acomete carnívoros domésticos. O desenvolvimento da doença por vezes depende do estado imunológico do animal e pode se desenvolver, na maioria dos casos, na forma subclínica e crônica. O Linfossarcoma de Sticker, também conhecido como Tumor Venéreo Transmissível (TVT), é uma neoplasia de células redondas que acomete principalmente a membrana mucosa da genitália externa. Este trabalho teve por objetivo descrever o diagnóstico de Hepatozoonose em cão associado ao tratamento quimioterápico para TVT. Foi atendido na Clínica Veterinária Escola da UFSC um cão macho, idoso, SRD, não castrado. Na anamnese foi relatado aumento do volume na base da região peniana com presença de sangramento pelo orifício prepucial. À exposição da glande do pênis, nada foi observado, mas, durante a palpação interna do prepúcio, foram notadas várias massas de aspecto polipoide que, por meio de citologia, evidenciaram TVT. O paciente iniciou quimioterapia com vincristina e, após a terceira sessão, iniciou com quadro clínico de emagrecimento, conjuntivite e blefarite. Ao hemograma, observou-se gamonte de H. canis em neutrófilo. O paciente foi tratado com doxiciclina e dipropionato de imidocarb associado à atropina e recuperou-se bem. O esfregaço sanguíneo foi uma ferramenta diagnóstica muito importante nesse caso, pois as formas de H. canis foram melhor observadas em decorrência do aumento da parasitemia causada pela imunossupressão do animal.

Canine hepatozoonosis, caused by the Hepatozoon spp, is a disease that affects domestic carnivores. The development of the disease sometimes depends on the animal's immune status and it can develop, in most cases, in the subclinical and chronic form. Sticker's Lymphosarcoma, also known as Transmissible Venereal Tumor(TVT), is a round cell neoplasma that mainly affects the mucous membrane of the external genitalia. This study aimed to describe the diagnosis of Hepatozoonosis in a dog associated with chemotherapy treatment for TVT. A male dog, elderly, SRD, not neutered was seen at the Veterinary Clinic of UFSC. In the anamnesis, an increase in volume at the base of the penile with bleeding through the preputial orifice was reported. Upon exposure of the glans penis, nothing was observed, but during the internal palpation of the foreskin, several polypoid masses were noticed, which, through cytology, showed TVT. The patient started chemotherapy with vincristine and, after the third session, started having weight loss, conjunctivitis and blepharitis. At hemogram, a gamonte of H. canis was observed in neutrophils. The patient was treated with doxycycline and atropine-associated imidocarb dipropionate and recovered well. The blood smear was a very important diagnostic tool in this case, as the forms of H. canis were better observed due to the increase in parasitemia caused by the animal's immunosuppression.

Hepatozoonose canina associada a quimioterapia para linfossarcoma de Sticker em Santa Catarina, Brasil / Canine hepatozoonosis associated with chemotherapy for Sticker's Lymphosarcoma in Santa Catarina, Brazil

A hepatozoonose canina, causada pelo protozoário Hepatozoon spp, é uma enfermidade que acomete carnívoros domésticos. O desenvolvimento da doença por vezes depende do estado imunológico do animal e pode se desenvolver, na maioria dos casos, na forma subclínica e crônica. O Linfossarcoma de Sticker, também conhecido como Tumor Venéreo Transmissível (TVT), é uma neoplasia de células redondas que acomete principalmente a membrana mucosa da genitália externa. Este trabalho teve por objetivo descrever o diagnóstico de Hepatozoonose em cão associado ao tratamento quimioterápico para TVT. Foi atendido na Clínica Veterinária Escola da UFSC um cão macho, idoso, SRD, não castrado. Na anamnese foi relatado aumento do volume na base da região peniana com presença de sangramento pelo orifício prepucial. À exposição da glande do pênis, nada foi observado, mas, durante a palpação interna do prepúcio, foram notadas várias massas de aspecto polipoide que, por meio de citologia, evidenciaram TVT. O paciente iniciou quimioterapia com vincristina e, após a terceira sessão, iniciou com quadro clínico de emagrecimento, conjuntivite e blefarite. Ao hemograma, observou-se gamonte de H. canis em neutrófilo. O paciente foi tratado com doxiciclina e dipropionato de imidocarb associado à atropina e recuperou-se bem. O esfregaço sanguíneo foi uma ferramenta diagnóstica muito importante nesse caso, pois as formas de H. canis foram melhor observadas em decorrência do aumento da parasitemia causada pela imunossupressão do animal.(AU)

Canine hepatozoonosis, caused by the Hepatozoon spp, is a disease that affects domestic carnivores. The development of the disease sometimes depends on the animal's immune status and it can develop, in most cases, in the subclinical and chronic form. Sticker's Lymphosarcoma, also known as Transmissible Venereal Tumor(TVT), is a round cell neoplasma that mainly affects the mucous membrane of the external genitalia. This study aimed to describe the diagnosis of Hepatozoonosis in a dog associated with chemotherapy treatment for TVT. A male dog, elderly, SRD, not neutered was seen at the Veterinary Clinic of UFSC. In the anamnesis, an increase in volume at the base of the penile with bleeding through the preputial orifice was reported. Upon exposure of the glans penis, nothing was observed, but during the internal palpation of the foreskin, several polypoid masses were noticed, which, through cytology, showed TVT. The patient started chemotherapy with vincristine and, after the third session, started having weight loss, conjunctivitis and blepharitis. At hemogram, a gamonte of H. canis was observed in neutrophils. The patient was treated with doxycycline and atropine-associated imidocarb dipropionate and recovered well. The blood smear was a very important diagnostic tool in this case, as the forms of H. canis were better observed due to the increase in parasitemia caused by the animal's immunosuppression.(AU)

Combinação da auto-hemoterapia e sulfato de vincristina no tratamento de tumor venéreo transmissível em cadelas em Moçambique / Combination of autohemotherapy and vincristine sulfate in treatment of canine transmissible venereal tumor in bitches in Mozambique

Background: Canine transmissible venereal tumor (TVT) is a tumor of round cells. Vincristine sulfate is the most effective for TVT. Alternatively, hemotherapy is an alternative therapy that consists of the administration of autologous blood and the positive effects are associated with an immunomodulatory effect. Since chemotherapy has some collateral effects, it is necessary to study another treatment with minimal side effects. In this context, this report case aimed to describe the use of autohemotherapy associated with vincristine sulfate for treating a transmissible venereal tumor in the vulvar mucosa of 7 adult bitches, being the first case report in Mozambique, Africa. Case: Seven adult bitches, median size, were referred to the School Veterinary Hospital, School of Veterinary, Eduardo Mondlane University, Maputo, Mozambique, Africa, with a diagnosis of TVT in the vulvar mucosa. All bitches were treated weekly with autohemotherapy and vincristine sulfate for 21 days. The parameters assessed included clinical and TVT macroscopic examination, complete blood count, serum biochemical examination and urinalysis, and were evaluated 60-min before each treatment. No clinical side effects were identified during the treatments. Color, appearance and tumor size were changed during the treatment period, and all bitches showed complete remission of the tumor 21 days after the beginning of treatment or after the third therapeutic session. The values of the complete blood count, serum biochemical and urinalysis did not demonstrate significant variations throughout the evaluated time-points. The TVT cytopathological classification was lymphocytic (42.9 %), plasmacytic (28.6 %) and lymphoplasmacytic (28.6 %). Discussion: The aims of this report were to describe the combination of autohemotherapy and vincristine sulfate for treating the transmissible venereal tumor located in the vulvar mucosa of adult bitches, through clinical and laboratory evaluation, and was not...

Ocorrência de tumor venéreo transmissível em cães atendidos no Hovet - PA UFRA / Occurrence of transmissible venereum tumor in dogs served at hove t- PA UFRA

The objective was to verify the occurrence of the transmissible venereal tumor in canines attended at (UFRA), Campus Belém / Pará, from March 2016 to September 2019. The information was obtained from the SISVET® program (Veterinary System). A total of 3,714 records were analyzed regarding the animals served and sent to the animal reproduction sector. Of this total, 155 were from dogs diagnosed with transmissible venereal tumor and 8 negative cases, 102 females and 53 males. The data were tabulated in an electronic spreadsheet, submitted to statistical analysis using the SAS® Universty Edition software regarding the frequency of occurrences, the influence of the age group, sex, race, through the Chi-Square test with 5% significance. Of the 155 cases of TVT diagnosed, 102 (65.81%) were in females and 53 (34.19%) males, with prevalence in SRD (72.86%) and in adults (54.19%). As for the site of involvement, 32.26% were in vulva and 20% in the body of the penis. Regarding the neighborhoods, there was a predominance in Guamá with 25 cases (15.59%). Thus, it is concluded that TVT was more frequent in SRD females and in adult dogs. Vulva 50 (32.26%), vagina 46 (29.68%) and penis 31 (20%) were the most affected sites.

Ocorrência de tumor venéreo transmissível em cães atendidos no Hovet - PA UFRA / Occurrence of transmissible venereum tumor in dogs served at hove t- PA UFRA

The objective was to verify the occurrence of the transmissible venereal tumor in canines attended at (UFRA), Campus Belém / Pará, from March 2016 to September 2019. The information was obtained from the SISVET® program (Veterinary System). A total of 3,714 records were analyzed regarding the animals served and sent to the animal reproduction sector. Of this total, 155 were from dogs diagnosed with transmissible venereal tumor and 8 negative cases, 102 females and 53 males. The data were tabulated in an electronic spreadsheet, submitted to statistical analysis using the SAS® Universty Edition software regarding the frequency of occurrences, the influence of the age group, sex, race, through the Chi-Square test with 5% significance. Of the 155 cases of TVT diagnosed, 102 (65.81%) were in females and 53 (34.19%) males, with prevalence in SRD (72.86%) and in adults (54.19%). As for the site of involvement, 32.26% were in vulva and 20% in the body of the penis. Regarding the neighborhoods, there was a predominance in Guamá with 25 cases (15.59%). Thus, it is concluded that TVT was more frequent in SRD females and in adult dogs. Vulva 50 (32.26%), vagina 46 (29.68%) and penis 31 (20%) were the most affected sites.(AU)