Aspectos anatomopatológicos e imuno-histoquímicos de cinomose em graxains / Anatomopathological and immunohistochemical aspects of distemper virus in crab-eating-foxes and pampa-foxes

Background: Distemper is a contagious disease with worldwide distribution, which is caused by a single-stranded RNAvirus of the genus Morbillivirus. The Crab-eating fox (Cerdocyon thous) and the Pampas fox (Lycalopex gymnocercus), wildcanids commonly found in the state of Rio Grande do Sul, are highly susceptible to the disease. Distemper virus infection oftenshows a multisystemic presentation. Central nervous system lesions are frequently reported, and may lead to death of wild canids.Distemper virus infection affecting these species has been cited by several studies; nonetheless, case descriptions involving theCrab-eating fox and the Pampas fox are infrequent. Therefore, the objective of the present study is to describe the anatomopathological and immunohistochemical aspects of distemper in the referred species, aiming to assist with the diagnosis of the disease.Materials, Methods & Results: A retrospective study was performed, and the necropsy reports of Crab-eating foxes and Pampasfoxes admitted to SPV-UFRGS from 2010 to 2016 were reviewed. Necropsy reports were revised in order to obtain information related to the clinical history and anatomopathological findings, and cases compatible with distemper were selected. Dataregarding the affected species, sex, approximate age, origin and clinical signs were compiled and analyzed. Selected histologicalsections of brain and cerebellum were submitted for immunohistochemistry (IHC) for monoclonal antibody canine distempervirus. In the studied period, 20 animals of the referred species were subjected to necropsy, of which four were diagnosed withdistemper as a cause of death. Of the affected animals, two were female and two were male. Three were juvenile and one wasan adult animal. Clinical signs reported included myoclonia, opisthotonos, forelimb paresis and nystagmus. No significant grosschanges were noted. Histopathological findings were restricted to the central nervous system...

Aspectos anatomopatológicos e imuno-histoquímicos de cinomose em graxains / Anatomopathological and immunohistochemical aspects of distemper virus in crab-eating-foxes and pampa-foxes

Background: Distemper is a contagious disease with worldwide distribution, which is caused by a single-stranded RNAvirus of the genus Morbillivirus. The Crab-eating fox (Cerdocyon thous) and the Pampas fox (Lycalopex gymnocercus), wildcanids commonly found in the state of Rio Grande do Sul, are highly susceptible to the disease. Distemper virus infection oftenshows a multisystemic presentation. Central nervous system lesions are frequently reported, and may lead to death of wild canids.Distemper virus infection affecting these species has been cited by several studies; nonetheless, case descriptions involving theCrab-eating fox and the Pampas fox are infrequent. Therefore, the objective of the present study is to describe the anatomopathological and immunohistochemical aspects of distemper in the referred species, aiming to assist with the diagnosis of the disease.Materials, Methods & Results: A retrospective study was performed, and the necropsy reports of Crab-eating foxes and Pampasfoxes admitted to SPV-UFRGS from 2010 to 2016 were reviewed. Necropsy reports were revised in order to obtain information related to the clinical history and anatomopathological findings, and cases compatible with distemper were selected. Dataregarding the affected species, sex, approximate age, origin and clinical signs were compiled and analyzed. Selected histologicalsections of brain and cerebellum were submitted for immunohistochemistry (IHC) for monoclonal antibody canine distempervirus. In the studied period, 20 animals of the referred species were subjected to necropsy, of which four were diagnosed withdistemper as a cause of death. Of the affected animals, two were female and two were male. Three were juvenile and one wasan adult animal. Clinical signs reported included myoclonia, opisthotonos, forelimb paresis and nystagmus. No significant grosschanges were noted. Histopathological findings were restricted to the central nervous system...(AU)

Papilomatose cutânea em cachorro-vinagre (Speothus venaticus Lund, 1842) - primeiro relato de caso / Cutaneous papillom atosis in a Bush Dog (Speothus venaticus Lund, 1842) first case report

Relata-se um caso de papilomatose cutânea em um cachorro-vinagre (Speothus venaticus) macho comidade de seis anos, pertencente ao plantel do Zoológico de Curitiba, Paraná. O paciente foi anestesiado pela associação de cetamina, xilazina e atropina, para avaliação clínica geral. Foram encontrados tumoreslocalizados em ambos os lados da face, especialmente ao redor das narinas, nos lábiose sob o olho direito.Essas lesões mediam 0,4 a 2,0 cm de diâmetro e até 0,5 cm de altura, e foram removidas cirurgicamente. O exame histopatológico mostrou que a epiderme apresentava hiperplasia irregular endo e exofítica com formações papiliformes curtas e irregulares, além de ortoqueratose compacta. A camada granulosa da epiderme exibia grânulos de queratohialina irregulares e grosseiros, sugestivos de causa viral. Os queratinócitos proliferados não apresentavam sinais importantes de atipia, e a derme subjacente apresentava edema e infiltrado inflamatório misto, em padrão perivascular a difuso.As lesões macroscópicas e microscópicaspossibilitaram o diagnóstico de papilomatose cutânea. Trata-se de uma doença comum em cães domésticos, mas este caso é o primeiro relatado em Speothus venaticus, canídeo neotropical ameaçado de extinção. Este artigo resgata um caso originalmente apresentado em 1998, no XVI Congresso Panamericano de Ciências Veterinárias (Santa Cruz de La Sierra, Bolivia). (AU)

It is reported a case of cutaneous papillomatosis in a six-year old, male Bush Dog (Speothus venaticus),from the Zoo of Curitiba, Paraná, Brazil. The patient was submitted to general anesthesia with the associationof ketamine hydrochloride, xylazine hydrochloride and atropine sulfate, for routine clinical examination.There were found tumors in both sides of the face, mostly around the nostrils, on the lips, andunder the right eye. They measured 0.4 to 2.0 cm in diameter and up to 0.5 cm in height, and were surgicallyremoved. Samples were collected and microscopically examined.Histologically, epidermis showedirregular endophytic and exophytic hyperplasia with short and irregular papilliform formations, aswell as compact orthokeratosis. The epidermal granulous layer showed irregular and coarse granules ofqueratohyalin, suggesting a viral etiology.The proliferative keratinocytes did not show significant signsof atypia, and underlying dermis has edema and mixed inflammatory infiltration in a perivascular todiffuse pattern. The macroscopic and microscopic features supported a diagnosis of cutaneous papillo-matosis. This is a fairly common disease in domestic dogs, but this case is the first one ever-reported inSpeothus venaticus, a neotropical canid endangered with extinction. This paper recovers a case originallypresented in 1998, at the XVI Pan-American Congress of Veterinary Sciences (Santa Cruz de la Sierra,Bolivia). (AU)

Papilomatose cutânea em cachorro-vinagre (Speothus venaticus Lund, 1842) - primeiro relato de caso / Cutaneous papillom atosis in a Bush Dog (Speothus venaticus Lund, 1842) – first case report

Relata-se um caso de papilomatose cutânea em um cachorro-vinagre (Speothus venaticus) macho comidade de seis anos, pertencente ao plantel do Zoológico de Curitiba, Paraná. O paciente foi anestesiado pela associação de cetamina, xilazina e atropina, para avaliação clínica geral. Foram encontrados tumoreslocalizados em ambos os lados da face, especialmente ao redor das narinas, nos lábiose sob o olho direito.Essas lesões mediam 0,4 a 2,0 cm de diâmetro e até 0,5 cm de altura, e foram removidas cirurgicamente. O exame histopatológico mostrou que a epiderme apresentava hiperplasia irregular endo e exofítica com formações papiliformes curtas e irregulares, além de ortoqueratose compacta. A camada granulosa da epiderme exibia grânulos de queratohialina irregulares e grosseiros, sugestivos de causa viral. Os queratinócitos proliferados não apresentavam sinais importantes de atipia, e a derme subjacente apresentava edema e infiltrado inflamatório misto, em padrão perivascular a difuso.As lesões macroscópicas e microscópicaspossibilitaram o diagnóstico de papilomatose cutânea. Trata-se de uma doença comum em cães domésticos, mas este caso é o primeiro relatado em Speothus venaticus, canídeo neotropical ameaçado de extinção. Este artigo resgata um caso originalmente apresentado em 1998, no XVI Congresso Panamericano de Ciências Veterinárias (Santa Cruz de La Sierra, Bolivia).

It is reported a case of cutaneous papillomatosis in a six-year old, male Bush Dog (Speothus venaticus),from the Zoo of Curitiba, Paraná, Brazil. The patient was submitted to general anesthesia with the associationof ketamine hydrochloride, xylazine hydrochloride and atropine sulfate, for routine clinical examination.There were found tumors in both sides of the face, mostly around the nostrils, on the lips, andunder the right eye. They measured 0.4 to 2.0 cm in diameter and up to 0.5 cm in height, and were surgicallyremoved. Samples were collected and microscopically examined.Histologically, epidermis showedirregular endophytic and exophytic hyperplasia with short and irregular papilliform formations, aswell as compact orthokeratosis. The epidermal granulous layer showed irregular and coarse granules ofqueratohyalin, suggesting a viral etiology.The proliferative keratinocytes did not show significant signsof atypia, and underlying dermis has edema and mixed inflammatory infiltration in a perivascular todiffuse pattern. The macroscopic and microscopic features supported a diagnosis of cutaneous papillo-matosis. This is a fairly common disease in domestic dogs, but this case is the first one ever-reported inSpeothus venaticus, a neotropical canid endangered with extinction. This paper recovers a case originallypresented in 1998, at the XVI Pan-American Congress of Veterinary Sciences (Santa Cruz de la Sierra,Bolivia).