Effect of partially replacing corn with sugar cane molasses on blood parameters and composition of the M. longissimus thoracis of growing pigs / Efeito da substituição parcial do milho por melaço de cana-de-açúcar sobre parâmetros sanguíneos e a composição do músculo longissimus thoracis de suínos em crescimento

The effect of sugar cane molasses, as a partial replacement to corn in the diet, on blood parameters and composition of the M. longissimus thoracis (LT) in growing pigs was explored in this study. Twenty female pigs aged 63 days, and weighing 28.98 ± 3.56 kg, were randomly assigned to either the control or sugar cane molasses treatments. Molasses was included at the 3% level to partially replace corn in their diet. Blood samples were collected at the beginning and end of the experiments. The animals were slaughtered at 110 days of age after 47 days in the experiment, weighing 67.9 ± 5.58 kg, and an LT muscle sample was extracted and evaluated. Each animal was considered an experimental unit. The treatment had no effect on the length and area of the LT muscle. Backfat thickness was reduced when using the sugar cane molasses treatment (5.80 mm) compared to the control treatment (8.90 mm) (P < 0.05). Higher enzyme gamma-glutamyl transferase (GGT) levels were observed in animals of the control treatment (67.10 IU/L) compared to animals treated with the sugar cane molasses treatment (49.90 IU/L) (P < 0.05). Moreover, the proximal composition, fatty acid profile, and quality were not influenced by treatment. Sugar cane molasses, used as an energy source to partially replace corn in the diet of growing pigs at a level of 3%, reduced the backfat thickness of the pig carcass and improved the serum concentration of the enzyme gamma-glutamyl transferase in pigs.(AU)

Neste estudo foi explorado o efeito do melaço de cana-de-açúcar em substituição parcial ao milho na dieta sobre os parâmetros sanguíneos e a composição do músculo longissimus thoracis (LT) de suínos em crescimento. Vinte leitoas com 63 dias de idade, pesando 28,98 ± 3,56 kg foram aleatoriamente distribuídas nos tratamentos controle ou melaço de canade-açúcar. O melaço foi incluído ao nível de 3% em substituição parcial ao milho na dieta. Ao início e ao final do experimento foram coletadas amostras de sangue dos animais. Os animais foram abatidos aos 110 dias de idade após 47 dias de experimento pesando 67,9 ± 5,58 kg e uma amostra do músculo LT foi extraída e avaliada. Cada animal foi considerado uma unidade experimental. Não houve diferença entre os tratamentos sobre o comprimento e a área do músculo LT. A espessura de toucinho foi reduzida ao utilizar o tratamento melaço de cana-de-açúcar (5,80 mm) em relação ao tratamento controle (8,90 mm) (P < 0,05). Níveis mais elevados da enzima gama-glutamil transferase (GGT) foram observados nos animais do tratamento controle (67,10 UI/L) em comparação aos animais do tratamento melaço de cana-de-açucar (49,90 UI/L) (P < 0,05). A composição proximal e o perfil e qualidade dos ácidos graxos não foram influenciados pelo tratamento. O melaço de cana-de-açúcar utilizado como fonte energética em substituição parcial ao milho na dieta de suínos em crescimento ao nível de 3% reduziu a espessura de toucinho da carcaça de suínos e melhorou a concentração sérica da enzima gama-glutamil transferase de suínos.(AU)

Proximal composition, fatty acid profile, omegas and lipid quality in the tambaqui (Colossoma macropomum Cuvier, 1818) (Serrasalmidae) in 'flatted cut' / Composição centesimal, perfil de ácidos graxos, ômegas e qualidade lipídica no tambaqui (Colossoma macropomum Cuvier, 1818) (Serrasalmidae) em 'corte espalmado'

The aimed of this study was to determine proximal composition, fatty acid profile, omegas and lipid quality in the tambaqui (Colossoma macropomum) in "flatted cut", produced and commercializeded by Rondônia state, Brazil. Three pieces of flatted tambaqui were collected from 40 fish weighing 1.10 ± 0.10 kg. Proximal composition were determined by AOAC Official method 969.23 and 968.08. The fatty acids were grouped to calculate ratio ∑PUFAs/∑SFAs and proportion ∑PUFAs (n-6/n-3), atherogenicity index (AI) were calculated, thrombogenicity index (TI), and ratio between hypocholesterolemic and hypercholesterolemic fatty acids (HH). Were found 1.06 g/100g of mineral matter, 17.64 g/100g of crude protein, 5.74 g/100g of lipids, 75 .54 g/100g of moisture and 122.22 kcal/100g of caloric value. Lipid composition 39.425% of SFAs, 43.518% of MUFAs and 17.057% of PUFAs. It also presented ∑PUFAs/∑SFAs ratios of 2.31 and ∑PUFAs (n-6/n-3) of 3.78. As AI of 0.44, TI of 0.92 and HH of 2.02. Tambaqui meat of 1.10 ± 0.10 kg in flatted cut contains good proximal contents, with high protein content and excellent lipid content, being considered a low-fat meat, with low moisture content and low caloric value and rich in minerals. It can also be considered a good source of MUFAs and PUFAs, especially n-3 and n-6, with high nutritional value, in addition to essential fatty acids, ALA, AA, DHA and EPA, which were crucial to provide good levels of lipid quality.(AU)

O objetivo deste estudo foi determinar a composição centesimal, perfil de ácidos graxos, ômegas e qualidade lipídica no tambaqui (Colossoma macropomum) "em corte espalmado", produzido e comercializado pelo estado de Rondônia, Brasil. Foram coletadas três amostras de 40 exemplares de tambaqui espalmado de 1,10 ± 0,10 kg. A composição centesimal foi deter-minada pelos métodos AOAC 969,23 e 968,08. Os ácidos graxos foram agrupados para calcular a razão ∑PUFAs/∑SFAs e a proporção ∑PUFAs (n-6/n-3), índice de aterogenicidade (IA), índice de trombogenicidade (IT) e razão entre ácidos graxos hipocolesterolêmico e hipercolesterolêmico (HH). Foram encontrados 1,06 g/100g de matéria mineral, 17,64 g/100g de pro-teína bruta, 5,74 g/100g de lipídios, 75,54 g/100g de umidade e 122,22 kcal/100g de valor calórico. Composição lipídica de 39,425% de SFAs, 43.518% de MUFAs e 12,057% de PUFAs. O corte também apresentou razões ∑PUFAs/∑SFAs de 2,31 e ∑PUFAs (n-6/ n -3) de 3,78. E também, AI de 0,44, TI de 0,92 e HH de 2,02. A carne de tambaqui de 1,10 ± 0,10 kg em corte espalmado contém bons teores centesimais, com alto teor proteico e excelente teor de lipídios, sendo considerada uma carne semi-gorda, com baixo teor de umidade e baixo valor calórico e rico em minerais. Também pode ser considerada uma boa fonte de MUFAs e PUFAs, principalmente n-3 e n-6, com alto valor nutricional, além de ácidos graxos essenciais, ALA, AA, DHA e EPA, que foram fundamentais para fornecer bons níveis de qualidade lipídica.(AU)

Morphology and immunolocalization of intertubular steroidogenic cell in mesonephros of Podocnemis expansa during gonadal differentiation

Sex steroid hormones are critical in gonadal differentiation in turtles. The gonads are not the only organs responsible for producing these hormones during this phase. Mesonephros play an important role in steroidogenesis. The present study aimed to investigate the presence of steroidogenic cells in mesonephros of Podocnemis expansa during gonadal differentiation and to evaluate their morphology and ultrastructure. Ten embryos of P. expansa were collected from 5 nests on day 36 of incubation, during spawning period on an artificial beach. Embryos were extracted from eggs by slicing the shell and euthanized. They were dissected under a stereoscopic microscope to collect the gonad-mesonephro complex, in which were fixed and subsequently processed for light microscopy, immunohistochemistry and transmission electron microscopy analysis. During histological analysis was observed mesonephros has typical morphological structure. Immunohistochemistry showed immunoreaction to aromatase in cells of intertubular space. Confirming these findings, it was possible to observe a type of intertubular cell in several regions of mesonephro, being more predominant in region close to blood vessels, distal and proximal tubules. In ultrastructural analysis these cells were characterized by having a clear, large, and rounded nucleus with evident nucleolus and cytoplasm rich in electron-dense droplets. This study demonstrated for the first time the presence of cells with morphological, immunohistochemical and ultrastructural characteristics similar to steroid-producing cells in P. expansa mesonephrons, suggesting that this organ may contribute to gonadal differentiation in this species.(AU)

Integrative taxonomy reveals a new species of Cyphocharax (Characiformes: Curimatidae) from the Upper Paraíba do Sul River basin, Brazil

A new species of Cyphocharax is described from the Upper Paraíba do Sul River basin, São Paulo, Brazil based on integrated morphological and molecular delimitation criteria. It is morphologically distinguished from its congeners by the presence of a round, dark blotch at the midlength of the caudal peduncle not extending to the proximal portions of the median caudal-fin rays, 19-20 circumpeduncular scales, 34-41 perforated lateral-line scales, 6-7 longitudinal scale rows above and below the lateral line, greatest body depth corresponding to 34.7-39.9% of standard length (SL), and the caudal peduncle depth corresponding to 13.3-15.2% of SL. The lowest genetic distances between the new species and other congeners are: 2.5% from C. gilbert, followed by 3.0% from C. santacatarinae, and 3.2% from C. aff. gilbert. All species delimitation criteria employed herein corroborated the recognition of the new species. In addition, comments on its conservation status are provided.(AU)

Uma nova espécie de Cyphocharax é descrita do alto rio Paraíba do Sul, São Paulo, Brasil, baseada em dados morfológicos e moleculares integrados. A espécie se distingue morfologicamente de seus congêneres por: apresentar uma mancha escura arredondada na porção média do pedúnculo caudal, não se estendendo à porção proximal dos raios medianos da nadadeira caudal, 19-20 escamas circunpedunculares, 34-41 escamas perfuradas na linha lateral, 6-7 escamas acima e abaixo da linha lateral, altura do corpo correspondendo à 34,7-39,9% do comprimento padrão (CP), e a altura do pedúnculo caudal correspondendo a 13,3-15,2% do CP. As menores distâncias genéticas entre a espécie nova e outras congêneres são: 2,5% de C gilbert, seguida de 3,0% de C. santacatarinae, e 3,2% de C. aff. gilbert. Todos os critérios de delimitação de espécies aqui empregados corroboraram o reconhecimento da nova espécie. Adicionalmente, comentários sobre seu estado de conservação são fornecidos.(AU)

Diffuse reflectance spectroscopy for estimating soil organic carbon and make nitrogen recommendations

Diffuse reflectance spectroscopy (DRS) has the potential to predict soil organic carbon (SOC). However, it is still little used as a matter of routine in soil laboratories in Brazil. The objective of this study was to make evaluations as to whether SOC predicted by spectral techniques can replace measurement by routine chemical methods with no loss in quality and be applied in the recommendation of nitrogen fertilizer as well as identifying the best prediction strategies to use. A data set containing 2,471 samples from six soil spectral libraries (SSL) was used to develop spectroscopic models for SOC content prediction, including consideration of sample stratification and preprocessing techniques. The SOC was quantified through the analytical-chemical methods of wet combustion with determination by titration, designated as the reference method (REM), and colorimeter, designated as the routine method (ROM in an independent data set). SOC contents predicted by the spectral analysis method (SAM) were compared to the REM and ROM results, converted to soil organic matter (SOM) and used for N recommendations. The best estimate for SOM content using the SAM was achieved through stratification of the SSL and application of the standard normal variate (SNV) preprocessing. The SOC predicted by spectral techniques proved capable of replacing the SOC measured by routine chemical methods with no loss of quality and supported by an appropriate nitrogen fertilizer recommendation, provided the models met the conditions and possessed the characteristics of the samples to be analyzed.

Diffuse reflectance spectroscopy for estimating soil organic carbon and make nitrogen recommendations

Diffuse reflectance spectroscopy (DRS) has the potential to predict soil organic carbon (SOC). However, it is still little used as a matter of routine in soil laboratories in Brazil. The objective of this study was to make evaluations as to whether SOC predicted by spectral techniques can replace measurement by routine chemical methods with no loss in quality and be applied in the recommendation of nitrogen fertilizer as well as identifying the best prediction strategies to use. A data set containing 2,471 samples from six soil spectral libraries (SSL) was used to develop spectroscopic models for SOC content prediction, including consideration of sample stratification and preprocessing techniques. The SOC was quantified through the analytical-chemical methods of wet combustion with determination by titration, designated as the reference method (REM), and colorimeter, designated as the routine method (ROM in an independent data set). SOC contents predicted by the spectral analysis method (SAM) were compared to the REM and ROM results, converted to soil organic matter (SOM) and used for N recommendations. The best estimate for SOM content using the SAM was achieved through stratification of the SSL and application of the standard normal variate (SNV) preprocessing. The SOC predicted by spectral techniques proved capable of replacing the SOC measured by routine chemical methods with no loss of quality and supported by an appropriate nitrogen fertilizer recommendation, provided the models met the conditions and possessed the characteristics of the samples to be analyzed.(AU)

Localization and expression of ADAM2 in the dromedary camel testis, epididymis and sperm during rutting season

ADAM2 (fertilin ) is a sperm surface protein reported in several mammalian species. However, the presence of ADAM2 in the male reproductive system and sperm of the camel is not well known. The present study was to clarify the localization and expression of ADAM2 in the dromedary camel testis, epididymis and spermatozoa during rutting season using immunohistochemistry (IHC) and the quantitative real-time polymerase chain reaction (qPCR). Tissue samples were obtained from the testis (proximal and distal) and epididymis (caput, corpus, and cauda) from eight mature male camels. Epididymal and ejaculated sperms were collected from four other fertile camels. IHC analysis clearly showed the localization of ADAM2 protein in the spermatocytes and the round and elongated spermatids of the testis, in the epithelial cells along the epididymis tract, on the posterior head of the sperm within the cauda epididymis, and on the acrosomal cap of both the epididymal and ejaculated sperm. The expression of camel ADAM2 mRNA was significantly higher (P 0.05) in the testis when compared with the epididymis. These findings may suggest an important role of ADAM2 in the fertility of male dromedary camels.(AU)

Região metafisária da tíbia de coelhos: uma opção para estudos dos substitutos ósseos / Metaphyseal region of the tibia of rabbits: an option for bone substitutes studies

Apresenta-se um modelo experimental de ostectomia de tíbia em coelhos para estudo de biomateriais em processos de reparação óssea. Foi realizada falha segmentar de 6mm de diâmetro na região metafisária medial proximal de tíbia de 72 coelhos as quais foram preenchidas com substituto ósseo e avaliadas clinicamente, por exame radiográfico e por meio de tomografia computadorizada feixe cônico, em diferentes tempos. Conclui-se que a metáfise medial proximal de tíbia de coelhos é adequada como modelo para estudos que avaliem o comportamento de enxertos e/ou biomateriais em falhas ósseas.

Is presented an experimental model of tibial ostectomy in rabbits to study biomaterials during bone repair process. Segmental failure of 6 mm diameter was performed in the medial proximal tibial metaphyseal region of 72 rabbits, which were filled with bone substitute and evaluated by clinical exam, X-ray, and cone beam computed tomography at different times. It is concluded that the medial proximal tibial metaphysis region of rabbits is suitable as a model for studies that evaluate the behavior of grafts and/or biomaterials on bone defects.

Ultrasonographic features of the normal thyroid gland in adult horses

ABSTRACT: Few reports have been published regarding the use of ultrasonography as a method of evaluating the normal thyroid gland in horses. For these reasons, this study aimed at determining reliably of the thyroid measurements from the comparison between the left and right thyroid lobes, as well as assessing the contour, format, echotexture and echogenicity of the healthy thyroid by mode-B ultrasonography. Additionally, the equine thyroid vascularization was quali-quantitatively characterized the by Doppler. The sample size initially was determined by the animal selection with advanced age and without volume increase in the neck proximal region. Finally, eleven horses were selected by laboratory test, search of thyroid neoformations by ultrasonography and cytology thyroid. Next, these animals were submitted to thyroid lobes ultrasonographic evaluation. Excellent reproducibility was observed for all measurements obtained. Among the comparisons made between the quantitative parameters of the left and right lobes, it was observed that there was only difference between their respective lengths. Qualitatively, there was a significant variation between the lobes elliptical format in the longitudinal plane of some glands, which oscillated between a rounded and flattened conformation. Thus, we can conclude that the difference between the lobes format of some thyroids can be explained by the significant difference observed between the length of the left and right lobes. Additionally, it was verified that there was no difference between the Doppler quantitative parameters. Therefore, we may suggest that unilateral analysis of the cranial thyroid artery by spectral Doppler can be used to evaluate equine thyroid diffuse disorders.

RESUMO: Poucas análises foram publicadas com relação ao uso da ultrassonografia como método de avaliação da glândula tireoide hígida em equinos. Alguns trabalhos incluem a determinação das dimensões e características do parênquima. Por essas razões, este estudo teve como objetivo determinar com segurança as medidas tireoidianas a partir da comparação entre o lobo tireoidiano esquerdo e direito, bem como avaliar os contornos, formato, ecotextura e ecogenicidade das glândulas tireoides hígidas pela ultrassonografia em modo B. Além disso, a vascularização da tireoide equina foi caracterizada quali-quantitativamente pela ferramenta Doppler. O tamanho da amostra inicialmente foi determinado pela seleção dos animais com idade avançada e sem aumento de volume na região proximal do pescoço. Por fim, onze equinos foram selecionados por meio de exame laboratorial, pesquisa de neoformações tireoidianas por ultrassonografia e citologia da tireoide. Em seguida, estes animais foram submetidos à avaliação de lobos tireoidianos por ultrassonografia. Observou-se excelente reprodutibilidade em relação a todas as medidas deste estudo. Foram observadas diferenças qualitativas entre os lobos tireoidianos direito e esquerdo. Assim, podemos concluir que tais diferenças entre o formato dos lobos tireoidianos podem ser explicadas pela diferença significativa e/ou tendência observada no parâmetro comprimento entre os respectivos lobos. Além disso, não foi encontrada diferença significativa entre os valores da artéria tireoidiana cranial esquerda em relação aos obtidos na artéria tireoidiana cranial direita, sugerindo que a análise dos valores espectrais do Doppler de uma das artérias tireoidianas craniais possa ser utilizada como método de avaliação de distúrbios difusos da tireoide equina.

Flesh quality and stress responses of Piaractus mesopotamicus after exposure to sublethal levels of ammonia and subsequent recovery / Qualidade da carne e respostas de estresse em Piaractus mesopotamicus após exposição à níveis subletais de amônia e subsequente recuperação

This study aimed to evaluate de effect of the exposure to sub-lethal levels of ammonia on blood parameters and flesh quality of juvenile pacu (Piaractus mesopotamicus), including assessment of such parameters post recovery in ammonia-free water. Juveniles (27.1 ± 5.4 g) were exposed to concentrations of ammonia at 0.0 (control); 0.5; and 1.0 mg N-NH3 L-1 for 10 days followed by the same period of recovery in ammonia-free water. On the 10th day post exposure and after a recovery period, samples of blood were taken for glucose, lactate analyses and evaluation of haematocrit. To evaluate lipid peroxidation, proximate composition and sensory analysis, samples of muscle/fillets were also obtained. Exposure to ammonia caused alterations in haematological response and negatively affected sensory analysis of pacu fillet. However, proximate composition was unchanged and lipid peroxidation process was not intensified in muscle. In conclusion, exposure to sublethal levels of ammonia induces secondary stress responses and altered the organoleptic characteristics of pacu flesh. Nevertheless, a recovery period of 10 days was sufficient to allow for a complete restoration of the homeostasis and organoleptic characteristics of the fillet.

O objetivo do presente estudo foi avaliar os efeitos da exposição a níveis subletais de amônia nos parâmetros sanguíneos e qualidade da carne de juvenis de pacu (Piaractus mesopotamicus). Além disso, também foi realizada a avaliação desses parâmetros pós-recuperação em água livre de amônia. Os peixes (27,1 ± 5,4 g) foram expostos a 0,0 (controle); 0,5 e 1,0 mg N-NH3 L-1 por 10 dias seguidos pelo mesmo tempo de recuperação em água livre de amônia. Ao final dos 10 dias de exposição e após o período de recuperação foram coletadas amostras de sangue para determinação dos níveis de glicose, lactato e dos valores de hematócrito. Para avaliação dos níveis de peroxidação lipídica, da composição proximal e realização da análise sensorial, amostras de músculo/filé também foram coletadas. A exposição à amônia ocasionou alterações nos parâmetros sanguíneos e afetou negativamente a análise sensorial dos filés de pacu. Entretanto, a composição proximal e o grau de peroxidação lipídica não foram alterados. Concluindo, a exposição a níveis subletais de amônia induziu respostas secundárias de estresse em juvenis de pacu e alterou características organolépticas do filé. Um período de recuperação de 10 dias foi suficiente para restaurar a homeostase e a qualidade do filé.

Flesh quality and stress responses of Piaractus mesopotamicus after exposure to sublethal levels of ammonia and subsequent recovery / Qualidade da carne e respostas de estresse em Piaractus mesopotamicus após exposição à níveis subletais de amônia e subsequente recuperação

This study aimed to evaluate de effect of the exposure to sub-lethal levels of ammonia on blood parameters and flesh quality of juvenile pacu (Piaractus mesopotamicus), including assessment of such parameters post recovery in ammonia-free water. Juveniles (27.1 ± 5.4 g) were exposed to concentrations of ammonia at 0.0 (control); 0.5; and 1.0 mg N-NH3 L-1 for 10 days followed by the same period of recovery in ammonia-free water. On the 10th day post exposure and after a recovery period, samples of blood were taken for glucose, lactate analyses and evaluation of haematocrit. To evaluate lipid peroxidation, proximate composition and sensory analysis, samples of muscle/fillets were also obtained. Exposure to ammonia caused alterations in haematological response and negatively affected sensory analysis of pacu fillet. However, proximate composition was unchanged and lipid peroxidation process was not intensified in muscle. In conclusion, exposure to sublethal levels of ammonia induces secondary stress responses and altered the organoleptic characteristics of pacu flesh. Nevertheless, a recovery period of 10 days was sufficient to allow for a complete restoration of the homeostasis and organoleptic characteristics of the fillet.(AU)

O objetivo do presente estudo foi avaliar os efeitos da exposição a níveis subletais de amônia nos parâmetros sanguíneos e qualidade da carne de juvenis de pacu (Piaractus mesopotamicus). Além disso, também foi realizada a avaliação desses parâmetros pós-recuperação em água livre de amônia. Os peixes (27,1 ± 5,4 g) foram expostos a 0,0 (controle); 0,5 e 1,0 mg N-NH3 L-1 por 10 dias seguidos pelo mesmo tempo de recuperação em água livre de amônia. Ao final dos 10 dias de exposição e após o período de recuperação foram coletadas amostras de sangue para determinação dos níveis de glicose, lactato e dos valores de hematócrito. Para avaliação dos níveis de peroxidação lipídica, da composição proximal e realização da análise sensorial, amostras de músculo/filé também foram coletadas. A exposição à amônia ocasionou alterações nos parâmetros sanguíneos e afetou negativamente a análise sensorial dos filés de pacu. Entretanto, a composição proximal e o grau de peroxidação lipídica não foram alterados. Concluindo, a exposição a níveis subletais de amônia induziu respostas secundárias de estresse em juvenis de pacu e alterou características organolépticas do filé. Um período de recuperação de 10 dias foi suficiente para restaurar a homeostase e a qualidade do filé.(AU)

Precision production environments for sugarcane fields

Sugarcane (saccharum spp.) in Brazil is managed on the basis of production environments. These production environments are used for many purposes, such as variety allocation, application of fertilizers and definition of the planting and harvesting periods. A quality classification is essential to ensure high economic returns. However, the classification is carried out by few and, most of the time, non-representative soil samples, showing unreal local conditions of soil spatial variability and resulting in classifications that are imprecise. One of the important tools in the precision agriculture technological package is the apparent electrical conductivity (ECa) sensors that can quickly map soil spatial variability with high-resolution and at low-cost. The aim of the present work was to show that soil ECa maps are able to assist classification of the production environments in sugarcane fields and rapidly and accurately reflect the yield potential. Two sugarcane fields (35 and 100 ha) were mapped with an electromagnetic induction sensor to measure soil ECa and were sampled by a dense sampling grid. The results showed that the ECa technique was able to reflect mainly the spatial variability of the clay content, evidencing regions with different yield potentials, guiding soil sampling to soil classification that is both more secure and more accurate. Furthermore, ECa allowed for more precise classification, where new production environments, different from those previously defined by the traditional sampling methods, were revealed. Thus, sugarcane growers will be able to allocate suitable varieties and fertilize their agricultural fields in a coherent way with higher quality, guaranteeing greater sustainability and economic return on their production.

Precision production environments for sugarcane fields

Sugarcane (saccharum spp.) in Brazil is managed on the basis of production environments. These production environments are used for many purposes, such as variety allocation, application of fertilizers and definition of the planting and harvesting periods. A quality classification is essential to ensure high economic returns. However, the classification is carried out by few and, most of the time, non-representative soil samples, showing unreal local conditions of soil spatial variability and resulting in classifications that are imprecise. One of the important tools in the precision agriculture technological package is the apparent electrical conductivity (ECa) sensors that can quickly map soil spatial variability with high-resolution and at low-cost. The aim of the present work was to show that soil ECa maps are able to assist classification of the production environments in sugarcane fields and rapidly and accurately reflect the yield potential. Two sugarcane fields (35 and 100 ha) were mapped with an electromagnetic induction sensor to measure soil ECa and were sampled by a dense sampling grid. The results showed that the ECa technique was able to reflect mainly the spatial variability of the clay content, evidencing regions with different yield potentials, guiding soil sampling to soil classification that is both more secure and more accurate. Furthermore, ECa allowed for more precise classification, where new production environments, different from those previously defined by the traditional sampling methods, were revealed. Thus, sugarcane growers will be able to allocate suitable varieties and fertilize their agricultural fields in a coherent way with higher quality, guaranteeing greater sustainability and economic return on their production.(AU)

Dexmedetomidine attenuates P2X4 and NLRP3 expression in the spine of rats with diabetic neuropathic pain

Purpose: To evaluate the effects of Dexmedetomidine (Dex) on spinal pathology and inflammatory factor in a rat model of Diabetic neuropathic pain (DNP). Methods: The rats were divided into 3 groups (eight in each group): normal group (N group), diabetic neuropathic pain model group (DNP group), and DNP model with dexmedetomidine (Dex group). The rat model of diabetes was established with intraperitoneal streptozotocin (STZ) injections. Nerve cell ultrastructure was evaluated with transmission electron microscopy (TEM). The mechanical withdrawal threshold (MWT) and motor nerve conduction velocity (MNCV) tests documented that DNP rat model was characterized by a decreased pain threshold and nerve conduction velocity. Results: Dex restored the phenotype of neurocytes, reduced the extent of demyelination and improved MWT and MNCV of DNP-treated rats (P=0.01, P=0.038, respectively). The expression of three pain-and inflammation-associated factors (P2X4, NLRP3, and IL-IP) was significantly upregulated at the protein level in DNP rats, and this change was reversed by Dex administration (P=0.0022, P=0.0092, P=0.0028, respectively). Conclusion: The P2X4/NLRP3 signaling pathway is implicated in the development and presence of DNP in vivo, and Dex protects from this disorder.(AU)

Ultrasonographic features of the normal thyroid gland in adult horses / Características ultrassonográficas da glândula tireoide hígida em equinos adultos

Few reports have been published regarding the use of ultrasonography as a method of evaluating the normal thyroid gland in horses. For these reasons, this study aimed at determining reliably of the thyroid measurements from the comparison between the left and right thyroid lobes, as well as assessing the contour, format, echotexture and echogenicity of the healthy thyroid by mode-B ultrasonography. Additionally, the equine thyroid vascularization was quali-quantitatively characterized the by Doppler. The sample size initially was determined by the animal selection with advanced age and without volume increase in the neck proximal region. Finally, eleven horses were selected by laboratory test, search of thyroid neoformations by ultrasonography and cytology thyroid. Next, these animals were submitted to thyroid lobes ultrasonographic evaluation. Excellent reproducibility was observed for all measurements obtained. Among the comparisons made between the quantitative parameters of the left and right lobes, it was observed that there was only difference between their respective lengths. Qualitatively, there was a significant variation between the lobes elliptical format in the longitudinal plane of some glands, which oscillated between a rounded and flattened conformation. Thus, we can conclude that the difference between the lobes format of some thyroids can be explained by the significant difference observed between the length of the left and right lobes. Additionally, it was verified that there was no difference between the Doppler quantitative parameters. Therefore, we may suggest that unilateral analysis of the cranial thyroid artery by spectral Doppler can be used to evaluate equine thyroid diffuse disorders.(AU)

Poucas análises foram publicadas com relação ao uso da ultrassonografia como método de avaliação da glândula tireoide hígida em equinos. Alguns trabalhos incluem a determinação das dimensões e características do parênquima. Por essas razões, este estudo teve como objetivo determinar com segurança as medidas tireoidianas a partir da comparação entre o lobo tireoidiano esquerdo e direito, bem como avaliar os contornos, formato, ecotextura e ecogenicidade das glândulas tireoides hígidas pela ultrassonografia em modo B. Além disso, a vascularização da tireoide equina foi caracterizada quali-quantitativamente pela ferramenta Doppler. O tamanho da amostra inicialmente foi determinado pela seleção dos animais com idade avançada e sem aumento de volume na região proximal do pescoço. Por fim, onze equinos foram selecionados por meio de exame laboratorial, pesquisa de neoformações tireoidianas por ultrassonografia e citologia da tireoide. Em seguida, estes animais foram submetidos à avaliação de lobos tireoidianos por ultrassonografia. Observou-se excelente reprodutibilidade em relação a todas as medidas deste estudo. Foram observadas diferenças qualitativas entre os lobos tireoidianos direito e esquerdo. Assim, podemos concluir que tais diferenças entre o formato dos lobos tireoidianos podem ser explicadas pela diferença significativa e/ou tendência observada no parâmetro comprimento entre os respectivos lobos. Além disso, não foi encontrada diferença significativa entre os valores da artéria tireoidiana cranial esquerda em relação aos obtidos na artéria tireoidiana cranial direita, sugerindo que a análise dos valores espectrais do Doppler de uma das artérias tireoidianas craniais possa ser utilizada como método de avaliação de distúrbios difusos da tireoide equina.(AU)

Ultrasonographic features of the normal thyroid gland in adult horses / Características ultrassonográficas da glândula tireoide hígida em equinos adultos

Few reports have been published regarding the use of ultrasonography as a method of evaluating the normal thyroid gland in horses. For these reasons, this study aimed at determining reliably of the thyroid measurements from the comparison between the left and right thyroid lobes, as well as assessing the contour, format, echotexture and echogenicity of the healthy thyroid by mode-B ultrasonography. Additionally, the equine thyroid vascularization was quali-quantitatively characterized the by Doppler. The sample size initially was determined by the animal selection with advanced age and without volume increase in the neck proximal region. Finally, eleven horses were selected by laboratory test, search of thyroid neoformations by ultrasonography and cytology thyroid. Next, these animals were submitted to thyroid lobes ultrasonographic evaluation. Excellent reproducibility was observed for all measurements obtained. Among the comparisons made between the quantitative parameters of the left and right lobes, it was observed that there was only difference between their respective lengths. Qualitatively, there was a significant variation between the lobes elliptical format in the longitudinal plane of some glands, which oscillated between a rounded and flattened conformation. Thus, we can conclude that the difference between the lobes format of some thyroids can be explained by the significant difference observed between the length of the left and right lobes. Additionally, it was verified that there was no difference between the Doppler quantitative parameters. Therefore, we may suggest that unilateral analysis of the cranial thyroid artery by spectral Doppler can be used to evaluate equine thyroid diffuse disorders.(AU)

Poucas análises foram publicadas com relação ao uso da ultrassonografia como método de avaliação da glândula tireoide hígida em equinos. Alguns trabalhos incluem a determinação das dimensões e características do parênquima. Por essas razões, este estudo teve como objetivo determinar com segurança as medidas tireoidianas a partir da comparação entre o lobo tireoidiano esquerdo e direito, bem como avaliar os contornos, formato, ecotextura e ecogenicidade das glândulas tireoides hígidas pela ultrassonografia em modo B. Além disso, a vascularização da tireoide equina foi caracterizada quali-quantitativamente pela ferramenta Doppler. O tamanho da amostra inicialmente foi determinado pela seleção dos animais com idade avançada e sem aumento de volume na região proximal do pescoço. Por fim, onze equinos foram selecionados por meio de exame laboratorial, pesquisa de neoformações tireoidianas por ultrassonografia e citologia da tireoide. Em seguida, estes animais foram submetidos à avaliação de lobos tireoidianos por ultrassonografia. Observou-se excelente reprodutibilidade em relação a todas as medidas deste estudo. Foram observadas diferenças qualitativas entre os lobos tireoidianos direito e esquerdo. Assim, podemos concluir que tais diferenças entre o formato dos lobos tireoidianos podem ser explicadas pela diferença significativa e/ou tendência observada no parâmetro comprimento entre os respectivos lobos. Além disso, não foi encontrada diferença significativa entre os valores da artéria tireoidiana cranial esquerda em relação aos obtidos na artéria tireoidiana cranial direita, sugerindo que a análise dos valores espectrais do Doppler de uma das artérias tireoidianas craniais possa ser utilizada como método de avaliação de distúrbios difusos da tireoide equina.(AU)

The Investigation of the Relationship Between HSP-27 Release and Oxidative DNA Damage in Broiler Chickens with Tibial Dyschondroplasia by Using Histopathological and Immunohistochemical Methods

Tibial dyschondroplasia (TD) is a skeletal disorder that occurs in the proximal metaphyses of tibiotarsus and sometimes tarsometatarsus, resulting in the development of avascularized and non-mineralized abnormal cartilage and causing significant economic loss. In this study, we aimed to show the histopathological changes and the relationship between the release of Heat-Shock Protein 27 (HSP-27) and oxidative DNA damage in broiler chickens with tibial dyschondroplasia, using histopathologic and immunohistochemical methods. Our study material consisted of totally 20 animals out of 42 days old 205 Ross 308 broiler chickens, 10 with TD lesions and 10 healthy control subjects. Tissue samples taken from animals performed necropsy was exposed to routine tissue follow-up. Macroscopically, unilateral and bilateral thickening and swelling were observed in the growth plates of tibiotarsal joints of the broiler chickens diagnosed with tibial dyscondroplasia. Histopathologic examination of the tibiotarsal joints of broiler chickens affected by TD revealed an increase in the number of immature chondrocytes, as well as deficiencies in vascularization and calcification. In the immunohistochemical study; HSP-27 and 8-OHDG release was positive in the chondrocytes located on the Proliferative Zone, Maturation Zone and Hypertrophic Zone. However, the positivity was the most profound in the PZ and MZ, while less in the HZ chondrocytes. As a result; we demonstrated by immunohistochemical methods that the increase in the HSP-27 release is parallel to the increase in 8-OHDG release in TD lesioned areas and this may be related to oxidative stress.(AU)

Evaluation of the accuracy and precision of the GE Dash 4000 oscillometric monitor for blood pressure measurement in anesthetized female dogs / Determinação da acurácia do monitor oscilométrico GE Dash 4000 na mensuração da pressão arterial em cadelas anestesiadas

Background: Indirect measurement of arterial blood pressure, such as the oscillometric method, is the most commonly used in clinical practice of dogs and cats. This method measures blood pressure values that are estimates of direct (invasive) arterial blood pressure values. Oscillometric devices are easy to use even for non-experienced personnel. However, there is considerable variation in accuracy and precision of blood pressure values measured by different oscillometric monitors. The present study aimed to determine the accuracy and precision of the GE Dash 4000 oscillometric monitor for arterial blood pressure measurement in anesthetized female dogs.Materials, Methods & Results:Sixteen healthy adult female dogs received 0.3 mg/kg morphine as premedication and were anesthetized with propofol and isoflurane. A 22-gauge catheter was introduced into the dorsal pedal artery and connected to a rigid tubular system and a pressure transducer filled with heparinized solution to allow direct (invasive) measurement of systolic (SAP), mean (MAP) and diastolic arterial pressure (DAP). A blood pressure cuff was positioned proximal to the carpus and connected to the oscillometric device (GE-DASH 4000 monitor) in order to obtain indirect measurements of SAP, MAP and DAP. Cuff width was 40% of limb circumference. During anesthesia, invasive arterial blood pressure values were measured and recorded simultaneously with the oscillometric method. The Bland Altman method was used to evaluate agreement between the methods by calculating the bias (invasive - oscillometric) and limits of agreement. Percentages of differences between the methods with an error ≤ 10 mmHg and ≤ 20 mmHg were calculated. Results were compared with the criteria from the American College of Veterinary Internal Medicine (ACVIM) for validation of noninvasive blood pressure methods.[...]

Evaluation of the accuracy and precision of the GE Dash 4000 oscillometric monitor for blood pressure measurement in anesthetized female dogs / Determinação da acurácia do monitor oscilométrico GE Dash 4000 na mensuração da pressão arterial em cadelas anestesiadas

Background: Indirect measurement of arterial blood pressure, such as the oscillometric method, is the most commonly used in clinical practice of dogs and cats. This method measures blood pressure values that are estimates of direct (invasive) arterial blood pressure values. Oscillometric devices are easy to use even for non-experienced personnel. However, there is considerable variation in accuracy and precision of blood pressure values measured by different oscillometric monitors. The present study aimed to determine the accuracy and precision of the GE Dash 4000 oscillometric monitor for arterial blood pressure measurement in anesthetized female dogs.Materials, Methods & Results:Sixteen healthy adult female dogs received 0.3 mg/kg morphine as premedication and were anesthetized with propofol and isoflurane. A 22-gauge catheter was introduced into the dorsal pedal artery and connected to a rigid tubular system and a pressure transducer filled with heparinized solution to allow direct (invasive) measurement of systolic (SAP), mean (MAP) and diastolic arterial pressure (DAP). A blood pressure cuff was positioned proximal to the carpus and connected to the oscillometric device (GE-DASH 4000 monitor) in order to obtain indirect measurements of SAP, MAP and DAP. Cuff width was 40% of limb circumference. During anesthesia, invasive arterial blood pressure values were measured and recorded simultaneously with the oscillometric method. The Bland Altman method was used to evaluate agreement between the methods by calculating the bias (invasive - oscillometric) and limits of agreement. Percentages of differences between the methods with an error ≤ 10 mmHg and ≤ 20 mmHg were calculated. Results were compared with the criteria from the American College of Veterinary Internal Medicine (ACVIM) for validation of noninvasive blood pressure methods.[...](AU)

Antioxidant activity and physicochemical analysis of passion fruit (Passiflora glandulosa Cav. ) pulp native to Cariri region / Atividade antioxidante e análise físico-química da polpa do maracujá (Passiflora glandulosa Cav. ) nativo da região do Cariri

The objective of this study was to determine the proximal composition, the physical and chemical characteristics and the in vitro antioxidant capacity (DPPH, ABTS and FRAP) of the pulp of the wild passion fruit (Passiflora glandulosa Cav.) from Cariri region, Ceara, Brazil. The results showed that the proximal composition and the caloric value of this passion fruit are similar to other species, but with a high ascorbic acid content. The fruit pulp showed low level of antioxidant activity and low level of polyphenolic compounds followed by three methodologies used. Due to high levels of titratable acidity (3.52) and total soluble solids (17.80 Brix), this fruit can be considered as a high value fruit for commerce. However, it is a species of passion fruit with few studies described in the literature, and more research is needed to assess its nutritional and functional potential.(AU)

O objetivo do estudo foi determinar a composição centesimal, as características físicas equímicas e a capacidade antioxidante (DPPH, ABTS e FRAP) in vitro da polpa do maracujá-do-mato(Passiflora glandulosa Cav.) da região do Cariri, Ceará, Brasil. Os resultados demonstraram que a composiçãocentesimal e o valor calórico desse tipo de maracujá são similares a outras espécies, mas com nível elevadode ácido ascórbico. A polpa apresentou baixo teor de polifenóis e baixa atividade antioxidante para as trêsmetodologias aplicadas. Em razão dos altos níveis de acidez titulável (3,52) e sólidos solúveis totais (17,80°Brix), este fruto pode ser considerado como um fruto de alto valor para o comércio. Porém, esta é umaespécie de maracujá pouco descrita na literatura, sendo necessárias mais análises para avaliar seu potencialvalor nutricional e funcional.(AU)