Effect of feeding frequency and water salinization on early development of Pyrrhulina brevis, an Amazonian ornamental fish / Efeito da frequência alimentar e da salinização da água sobre o desenvolvimento inicial de Pyrrhulina brevis, um peixe ornamental da Amazônia

Pyrrhulina brevis is an endemic fish from the Amazon basin that is valued in the ornamental fish market. In larviculture, fish are very sensitive to stressors and have a high mortality rate. Salinized water may provide more energy to overcome stress and feeding frequency is an important strategy to improve productive performance. Therefore, the aim of this study was to evaluate the best feeding frequency and the best level of water salinization for larval development of P. brevis. A total of 360 post-larvae of P. brevis (5.26 ± 1.65 mg, 5.57 ± 0.68 mm) were randomly distributed in 36 aquariums (1 L) in a 3x4 factorial scheme, to evaluate three concentrations of salt in water (0, 1 and 2 g L-1) and four feeding frequencies (once, twice, three and four times a day). We offered 150 Artemia nauplii per day and per post-larvae during 15 days. The fish kept at 1 g L-1 salinized water, and those fed 3 and 4 times a day presented significantly greater final length and weight, weight gain, and specific growth rate for length and weight. An interaction between salinized water and feeding frequency was observed for survival rate. We conclude that, during the initial rearing phase of P. brevis, feeding frequency of three times a day and salinized water at 1 g L-1 is recommended.(AU)

Pyrrhulina brevis é um peixe endêmico da bacia amazônica que apresenta bons valores no mercado de peixes ornamentais. Na larvicultura, os peixes são muito sensíveis aos estressores e apresentam alta taxa de mortalidade. A água salinizada pode fornecer mais energia para superar o estresse e a frequência alimentar é uma estratégia importante para melhorar o desempenho produtivo. Portanto, o objetivo deste trabalho foi avaliar a melhor frequência alimentar e o melhor nível de salinização da água para o desenvolvimento larval de P. brevis. Um total de 360 pós-larvas de P. brevis (5,26 ± 1,65 mg, 5,57 ± 0,68 mm) foram distribuídos aleatoriamente em 36 aquários (1 L) em esquema fatorial 3x4, para avaliar três concentrações de sal na água (0, 1 e 2 g L-1) e quatro frequências de alimentação (uma, duas, três e quatro vezes ao dia). Foram oferecidas 150 náuplios de Artemia por dia e por pós-larva, durante 15 dias. Os peixes mantidos com 1 g L-1 de água salinizada e os alimentados 3 e 4 vezes ao dia apresentaram comprimento e peso finais, ganho de peso e taxa de crescimento específico para comprimento e peso significativamente maiores. Uma interação entre água salinizada e frequência alimentar foi observada para a taxa de sobrevivência. Concluímos que, durante a fase inicial de criação de P. brevis, recomenda-se uma frequência alimentar de três vezes ao dia e água salinizada a 1 g L-1.(AU)

Influence of nutritional management prior to adaptation to a feedlot diet on ruminal microbiota of Nellore cattle

ABSTRACT The objective of this study was to evaluate the effect of either a limited forage intake or concentrate supplementation prior to the adaptation to high-concentrate diets on dry matter intake, ruminal pH, bacteria, and protozoa of Nellore cattle. The experiment was designed as a two 3×3 Latin square, and six cannulated Nellore steers were used. Each experimental period was composed by three feeding phases: pre-adaptation (14 days), adaptation (12 days), and finishing (seven days) diet, in a total of 33 days per period. The steers were assigned to one of three pre-adaptation dietary treatments: control (Tifton hay fed ad libitum + mineral supplement), restriction (Tifton hay fed at 1.4% of BW + mineral supplement), and concentrate (Tifton hay fed ad libitum + 0.5% of BW of a mix of concentrate feedstuffs and mineral supplement). The adaptation period consisted of two adaptation diets, which contained 72 and 79% concentrate for six days each. The finishing diet contained 86% concentrate. During the pre-adaptation phase, restricted cattle had higher pH than concentrate-fed cattle. There was a reduction in M. elsdenii relative population in cattle from either restriction or concentrate groups. During adaptation and finishing phases, cattle from concentrate group had smaller F. succinogenes populations compared with the control group. The previous nutritional backgrounds impact ruminal microbiota during adaptation and finishing phases without causing any negative effect on ruminal pH. Feeding concentrate prior to the adaptation positively impacted the transition to high-concentrate diets and promoted increased dry matter intake.

Ammonium dipropionate in the total mixed ration does not change the ingestive behavior but improves the productive performance of feedlot bulls / A adição do dipropionato de amônio na ração total misturada não altera o comportamento ingestivo, mas melhora o desempenho produtivo de novilhos confinados

The objective of the present study was to evaluate the productive performance, the ingestive behavior, the apparent digestibility of the diet, and the carcass characteristics of beef steers finished in confinement under the effect of ammonium dipropionate in the diet, and the fractionation or not in the supply of the diet. The experimental design was completely randomized, in a 2 x 2 factorial scheme, totaling four treatments, as follows: Diet without ammonium dipropionate provided twice a day; Diet without ammonium dipropionate given once daily; Diet with ammonium dipropionate provided twice daily; Ammonium dipropionate diet provided once daily. Thirty-two ½ Angus ½ Nellore bulls were used, with an average age of 11 months. The use of ammonium dipropionate in the overall average increased average daily gain, dry matter intake, and carcass gain. The diet provided twice a day provided, on average, greater weight gain, greater carcass gain, and better feed conversion. When evaluating the association between treatments, the use of dipropionate plus the diet supplied twice a day showed greater daily carcass gain during the experimental period and higher hot carcass weight (1.251 kg, 111.4 kg, and 308.6 kg respectively), as well as ensuring better apparent digestibility of dry matter (74.57%). With the data obtained in the present study, it is possible to state that it is advisable to use ammonium dipropionate while maintaining the fractionation of the diet for beef steers in the finishing phase.(AU)

O objetivo do presente estudo foi avaliar o desempenho produtivo, o comportamento ingestivo, a digestibilidade aparente da dieta e as características de carcaça de novilhos de corte terminados em confinamento sob efeito do dipropionato de amônio na dieta, e do fracionamento ou não no fornecimento da dieta. O delineamento experimental foi inteiramente casualizado, em um esquema fatorial 2 x 2, totalizando quatro tratamentos, sendo: Dieta sem dipropionato de amônio fornecida duas vezes ao dia; Dieta sem dipropionato de amônio fornecida uma vez ao dia; Dieta com dipropionato de amônio fornecida duas vezes ao dia; Dieta com dipropionato de amônio fornecida uma vez ao dia. Utilizou-se 32 novilhos ½ sangue Angus ½ sangue Nelore, com idade média de 11 meses. O uso do dipropionato de amônio na média geral aumentou o ganho médio diário, a ingestão de matéria seca, e o ganho de carcaça. A dieta fornecida duas vezes ao dia proporcionou na média geral, maior ganho de peso, maior ganho de carcaça e melhor conversão alimentar. Ao avaliar a associação entre os tratamentos, o uso de dipropionato mais a dieta fornecida duas vezes ao dia mostrou maior ganho de carcaça diário, durante o período experimental e maior peso de carcaça quente (1,251 kg, 111,4 kg e 308,6 kg respectivamente), assim como garantiu melhor digestibilidade aparente da matéria seca (74,57%). Com os dados obtidos no presente estudo é possível afirmar que é recomendável utilizar o dipropionato de amônio mantendo o fracionamento da dieta para novilhos de corte em fase de terminação.(AU)

Applicability of management tools in a family dairy farm: a case study / Aplicabilidade de ferramenta de gestão em propriedade leiteira familiar: estudo de caso

This study aimed to describe the application of the management tool GUT matrix in a family dairy farm in the state of Acre to identify the main weaknesses and propose adequate tools to allow better management of the causes of the three biggest identified problems. This is a case study carried out on a single property located in the municipality of Rio Branco - Acre in May 2022. Information was collected by applying a semi-structured form with 267 questions. The GUT matrix allo-wed ranking the three main weaknesses of the property, namely: "lack of zootechnical bookkeeping," "long calving interval," and "failure in feeding management." The management tools proposed to the producer, seeking to guide and organize the activities of the property, consisted of 5W2H and Ishikawa diagram.(AU)

Objetivou-se descrever a aplicação da ferramenta de gestão, "matriz GUT", em uma propriedade leiteira familiar no estado do Acre para identificar os principais pontos fracos e propor ferramentas adequadas para permitir melhor gerenciamento das causas dos 3 maiores problemas identificados. Trata-se de um estudo de caso realizado em propriedade única localizada no município de Rio Branco - AC em maio de 2022, as informações foram coletadas por meio de formulário semiestruturado contendo 267 questões. A matriz GUT permitiu ranquear os 3 principais pontos fracos da propriedade, sendo: "falta de escrituração zootécnica", "intervalo entre partos longo" e "Falha no manejo alimentar". As ferramentas de gestão propostas ao produtor, buscando orientar e organizar as atividades da propriedade consistiram no 5W2H e Diagrama de Ishikawa.(AU)

Effect of lactic acid bacteria preparations on calf fecal flora

This experiment was conducted to investigate the effects of lactic acid bacteria preparations on microbial diversity and community structure of calves. On days 1 and 7 of the trial period, feces were collected into sterile tubes and labeled (Day 1: control group D1DZ, experimental group D1SY, and Day 7: control group D7DZ, experimental group D7SY). Twenty Angus calves (150±10 kg) were selected and randomly divided into two groups of 10 calves each. The control group fed a basal diet. In addition to feeding the basal diet, the experimental group was given 15 mL lactobacillus preparation orally at 09:00 and 16:00 h every day. Calves were allowed free feeding and drinking water. All other feeding environments and management conditions remained consistent with the experiment lasting for seven days. At the end of the experiment, the fecal microflora of the calves was analyzed using 16S rRNA sequencing techniques. The 16S rRNA analysis data were processed using the Excel 2007 software and analyzed by the IBM SPSS statistical software (Statistical Analysis System, version 22). The Alpha diversity index analysis showed that the Chao and the Ace indices were significantly different after feeding supplemented with lactic acid bacteria. The PCA analysis showed that the fecal flora structure differed significantly after supplementation with the lactic acid bacteria preparation. Further analysis showed that the lactic acid bacteria increased Firmicutes, Patescibacteria, Rikenellaceae_RC9_gut_group, Clostridium_sensu_stricto_1, and prevotellaceae_UCG-003 in the feces. Therefore, we speculate that lactic acid bacteria preparations play an important role in animal production and are beneficial to the diversity of the fecal microflora of the calves.

Effects of mixed feeding strategies with different dietary energy: protein ratios on juvenile Nile tilapia (Oreochromis niloticus) / Efeitos de estratégias de alimentação mista com diferentes relações de energia: proteína para juvenis de tilápia-do-Nilo (Oreochromis niloticus)

The evaluation of feeding strategies is necessary to ensure the sustainability of aquaculture. This study assessed the effect of two diets with different E:P ratios (9.6 and 10.3 kcal of digestible energy per gram of crude protein) on Nile tilapia juveniles. The growth, feed and nutrient use, economic parameters, whole-body composition, and liver steatosis of fish were evaluated. There was no significant effect of treatments on the growth, feed intake, feed conversion ratio, uniformity, and survival of the fish. The lower feed cost (P>0.05) per biomass or 1000 units produced was registered in Nile tilapia juveniles fed with 10.3 kcal DE/g CP diet for seven days. The contribution of ether extract in fish weight gain was reduced (P=0.055) by the increased use of the 10.3 kcal DE/g CP diet in the feeding strategies. The same trend was observed in fish whole-body lipid levels. Body indexes were similar (P>0.05) among fish from the different treatments. Mixing diets with different E:P ratios in a weekly feeding protocol does not impair productive performance of Nile tilapia juveniles. However, considering the cost of feeding, the recommendation is to supply a diet with 33%CPand 3.4 kcal/DE for seven days per week.(AU)

A avaliação das estratégias de alimentação é necessária para garantir a sustentabilidade da aquicultura. Este estudo avaliou o efeito de duas dietas com diferentes proporções de E:P (9,6 e 10,3 kcal de energia digestível por grama de proteína bruta) para juvenis de tilápia do Nilo. Foram avaliados o crescimento, uso da dieta e nutrientes, parâmetros econômicos, composição corporal e esteatose hepática de peixes. Não houve efeito significativo dos tratamentos sobre o crescimento, consumo de ração, conversão alimentar, uniformidade e sobrevivência dos peixes. O menor custo de ração (P>0,05) por biomassa ou 1000 unidades produzidas foi registrado nos juvenis de tilápia do Nilo alimentados com a dieta 10,3 kcal DE/g PB por sete dias. A contribuição do extrato etéreo no ganho de peso dos peixes foi reduzida (P=0,055) pela maior utilização da dieta 10,3 kcal DE/g PB nas estratégias de alimentação utilizadas. A mesma tendência foi observada nos níveis de lipídios corporais em peixes. Os índices corporais foram semelhantes (P>0,05) entre os peixes dos diferentes tratamentos. A mistura de dietas com diferentes relações E:P em um protocolo de alimentação semanal não prejudica o desempenho produtivo de juvenis de tilápia do Nilo. No entanto, considerando os custos de alimentação, a recomendação é fornecer uma dieta com 33% PB e 3,4 kcal/DE por sete dias por semana.(AU)

Optimizing gilt mammary development to increase future milk yield

Sows do not produce enough milk to sustain optimal growth of their litters. This is particularly important when considering the current hyperprolific sow genetic lines. Mammary development needs to be considered to improve potential milk yield. One can only attempt to stimulate mammogenesis during periods when rapid mammary development is already ongoing. There are two such periods before lactation starts, namely, from three months of age until puberty, and from 90 days of gestation until farrowing. Early studies showed that a 20% feed restriction from 90 days of age until puberty drastically reduces mammary parenchymal tissue mass. Yet, in a more recent study, sow milk yield was not altered following a 10% or 20% feed restriction, or a 25% dietary fibre addition from 90 days of age to breeding. This absence of effect was likely due to the greater feed intake of control gilts in that recent study compared with the older studies, and suggested that feed intake of growing gilts can be reduced to 2.7 kg/d (but not 2.1 kg/d) without detrimental effects on future milk yield. During prepuberty, inclusion of the phytoestrogen genistein in the diet increases the number of mammary parenchymal cells. During late gestation, feeding very high energy levels may have detrimental effects on mammary development and subsequent milk production. Feed intake throughout gestation is also important because of its effect on body condition, with gilts that are too thin (< 16 mm backfat thickness) in late gestation showing less mammary development. A 40% increase in lysine intake via inclusion of additional soybean meal to the diet of gilts from days 90 to 110 of gestation increased mammary parenchymal mass by 44%. Increasing circulating concentrations of the growth factor IGF-1 during late gestation also increased mammary parenchymal mass by 22%. Current data clearly demonstrate that feeding management before lactation can be used to enhance mammary development, hence future milk yield.(AU)

Anthelmintic usage on the Reproductive Parameters in Captive reared Agoutis (Dasyprocta leporina) in Trinidad and Tobago, West Indies

Abstract The agouti (Dasyprocta leporina) is a rodent that is found in the Neo-tropical region. This animal is hunted for its meat but has recently been reared in captivity as a source of meat protein in rural communities. A 20-month experiment was carried out to evaluate the effect of an anthelmintic on the reproductive performance of the agouti (Dasyprocta leporina) reared in captivity. This experiment was conducted in the humid tropics of Trinidad and Tobago. Sixteen animals (15 females, 1 male) placed in each of the two treatment groups in a completely randomized study design. In treatment 1 (T1) animals were given subcutaneous injections of Endovet Ces® (Ivermectin/Praziquantel) at 0.2 mg/kg every three months. Treatment 2 (T2) was the negative control group where animals were not exposed to an anthelmintic. Reproductive data were collected at parturition which included birth weight, litter weight, litter size and gender of offspring. The results showed that there was no statistical difference (p > 0.05) between the treatment groups with respect to birth weight, litter weight, litter size and gender. However, agoutis that were dewormed had a higher birth weight (220.24 g vs 209.1 g) and litter weight (369.8 g vs 343 g). The same values were obtained for the litter size (1.7 vs 1.7) and animals that were dewormed had a higher female offspring to male offspring (2.41:1 vs 1.11:1). This experiment demonstrated that the use of an anthelmintic strategically in the management of captive reared agoutis had no statistical effect (p > 0.05) on the reproductive parameters. Therefore, these animals can be kept in captive conditions without being dewormed and produce efficiently with proper feeding and housing management.

Resumo A cutia (Dasyprocta leporina) é um roedor que se encontra na região neo-tropical. Esse animal é caçado por sua carne, mas recentemente foi criado em cativeiro como fonte de proteína de carne em comunidades rurais. Um experimento de 20 meses foi realizado para avaliar o efeito de um anti-helmíntico no desempenho reprodutivo de cutias (Dasyprocta leporina) criadas em cativeiro. Esse experimento foi conduzido nos trópicos úmidos de Trinidad e Tobago. Dezesseis animais (15 fêmeas, 1 macho) colocados em cada um dos dois grupos de tratamento em um desenho de estudo completamente randomizado. No tratamento 1 (T1) os animais receberam injeções subcutâneas de Endovet Ces® (Ivermectina / Praziquantel) na dose de 0,2 mg / kg a cada três meses. O tratamento 2 (T2) foi o grupo de controle negativo onde os animais não foram expostos a um anti-helmíntico. Os dados reprodutivos foram coletados no parto, incluindo peso ao nascer, peso da ninhada, tamanho da ninhada e sexo da prole. Os resultados mostraram que não houve diferença estatística (p > 0,05) entre os grupos de tratamento com relação ao peso ao nascer, peso da ninhada, tamanho da ninhada e sexo. No entanto, cutias desparasitadas tiveram maior peso ao nascer (220,24 g vs. 209,1 g) e peso da ninhada (369,8 g vs. 343 g). Os mesmos valores foram obtidos para o tamanho da ninhada (1,7 vs. 1,7) e os animais que foram desparasitados tiveram uma prole feminina maior do que a prole masculina (2,41: 1 vs. 1,11: 1). Esse experimento demonstrou que o uso de anti-helmíntico estrategicamente no manejo de cutias criadas em cativeiro não teve efeito estatístico (p > 0,05) sobre os parâmetros reprodutivos. Portanto, esses animais podem ser mantidos em cativeiro sem serem vermifugados e produzir de forma eficiente com alimentação adequada e manejo do alojamento.

Effect of temperature on embryonic development and first exogenous feeding of goldfish Carassius auratus (Linnaeus, 1758) / Efeito da temperatura no desenvolvimento embrionário e na primeira alimentação exógena do goldfish Carassius auratus (Linnaeus, 1758)

Goldfish or Kinguio is a widely marketed species worldwide due to the ornamental market. There is some lack of acknowledgment of the production of the species under specific climatic conditions. To evaluate the effect of temperature on embryonic development and the first exogenous feeding of goldfish, an experiment was proposed. Fifteen incubators, organized in five treatments (18, 22, 26, 30, and 34 °C) with three replications each, were used to keep the fertilized goldfish eggs until the first exogenous feeding of the larvae. The main development events were observed to understand the possible effects of these temperatures on embryos and larvae of the species. Temperature influences embryo development and the time of first exogenous feeding of goldfish. The temperature of 34 °C was lethal to the species causing 100% of anomalies in the embryos and larvae. The experiment data allow us to conclude that the species presents a maximum thermal limit during embryogenesis, and these data are important to the aquaculture industry and to understand the effect of climate changes on goldfish. The data obtained in this experiment will assist in the management of invasive species and production of the species (aquaculture).

O peixe dourado ou Kinguio é uma espécie amplamente comercializada em todo o mundo devido ao mercado ornamental. Existe alguma falta de conhecimento da produção da espécie em condições climáticas específicas. Para avaliar o efeito da temperatura no desenvolvimento embrionário e na primeira alimentação exógena do Kinguio, um experimento foi proposto. Quinze incubadoras, organizadas em cinco tratamentos (18, 22, 26, 30 e 34 °C) com três repetições cada, foram utilizadas para manter os ovos fertilizados de Kinguio até a primeira alimentação exógena das larvas. Os principais eventos do desenvolvimento foram observados para entender os possíveis efeitos dessas temperaturas em embriões e larvas da espécie. A temperatura influencia o desenvolvimento do embrião e o tempo da primeira alimentação exógena do Kinguio. A temperatura de 34°C foi letal para a espécie causando 100% de anomalias nos embriões e larvas. Os dados do experimento permitem concluir que a espécie apresenta um limite térmico máximo durante a embriogênese, sendo esses dados importantes para a indústria da aquicultura e para entender o efeito das mudanças climáticas no Kinguio. Os dados obtidos neste experimento auxiliarão no manejo de espécies invasoras e na produção da espécie (aquicultura).

Zoo visitors as a source of enrichment to reduce abnormal behavior in captive rhesus macaques (Macaca mulatta) in the Central Zoo, Kathmandu, Nepal

Overexpression of abnormal behavior among captive primates indicates poor management practices. The type, frequency, and contributing factors of abnormal behaviors vary highly across individual animals in captive settings. This study explored if sex, rearing history, the number of visitors, and type of visitor-monkey interactions affected the behaviors of captive rhesus macaques (Macaca mulatta) housed at the Central Zoo in Kathmandu, Nepal. Behavioral observations of six adult rhesus macaques (ages 4­12 years; two were rescued from the wild, and four were born in the zoo) were conducted using the focal animal sampling method. Observations were collected daily in two conditions (7:00­10:00 AM, without visitors present; and 10:00 AM­1:00 PM, with visitors present). During the visitor presence condition, instantaneous scan sampling also was implemented every 10 minutes to record the number of visitors and the type of visitor-monkey interaction. Resting, feeding and grooming were the most prevalent activities, and abnormal behavior ranked fifth throughout the observation period. Significant differences were observed in abnormal or stress-related behavior by sex and rearing history: females engaged in abnormal behavior more than the males, and rescued monkeys engaged in more abnormal behavior than the captive born. Abnormal behavior decreased, begging behavior increased as the number of zoo visitors and the extent of visitor-monkey interaction increased. We conclude that captive rhesus macaques respond to zoo visitors as a source of enrichment, and visitors' presence helps decrease abnormal or stress-related behavior levels.

Anthelmintic usage on the Reproductive Parameters in Captive reared Agoutis (Dasyprocta leporina) in Trinidad and Tobago, West Indies / Uso de anti-helmíntico nos parâmetros reprodutivos em cutias criadas em cativeiro (Dasyprocta leporina) em Trinidad e Tobago, Índias Ocidentais

Abstract The agouti (Dasyprocta leporina) is a rodent that is found in the Neo-tropical region. This animal is hunted for its meat but has recently been reared in captivity as a source of meat protein in rural communities. A 20-month experiment was carried out to evaluate the effect of an anthelmintic on the reproductive performance of the agouti (Dasyprocta leporina) reared in captivity. This experiment was conducted in the humid tropics of Trinidad and Tobago. Sixteen animals (15 females, 1 male) placed in each of the two treatment groups in a completely randomized study design. In treatment 1 (T1) animals were given subcutaneous injections of Endovet Ces® (Ivermectin/Praziquantel) at 0.2 mg/kg every three months. Treatment 2 (T2) was the negative control group where animals were not exposed to an anthelmintic. Reproductive data were collected at parturition which included birth weight, litter weight, litter size and gender of offspring. The results showed that there was no statistical difference (p > 0.05) between the treatment groups with respect to birth weight, litter weight, litter size and gender. However, agoutis that were dewormed had a higher birth weight (220.24 g vs 209.1 g) and litter weight (369.8 g vs 343 g). The same values were obtained for the litter size (1.7 vs 1.7) and animals that were dewormed had a higher female offspring to male offspring (2.41:1 vs 1.11:1). This experiment demonstrated that the use of an anthelmintic strategically in the management of captive reared agoutis had no statistical effect (p > 0.05) on the reproductive parameters. Therefore, these animals can be kept in captive conditions without being dewormed and produce efficiently with proper feeding and housing management.

Resumo A cutia (Dasyprocta leporina) é um roedor que se encontra na região neo-tropical. Esse animal é caçado por sua carne, mas recentemente foi criado em cativeiro como fonte de proteína de carne em comunidades rurais. Um experimento de 20 meses foi realizado para avaliar o efeito de um anti-helmíntico no desempenho reprodutivo de cutias (Dasyprocta leporina) criadas em cativeiro. Esse experimento foi conduzido nos trópicos úmidos de Trinidad e Tobago. Dezesseis animais (15 fêmeas, 1 macho) colocados em cada um dos dois grupos de tratamento em um desenho de estudo completamente randomizado. No tratamento 1 (T1) os animais receberam injeções subcutâneas de Endovet Ces® (Ivermectina / Praziquantel) na dose de 0,2 mg / kg a cada três meses. O tratamento 2 (T2) foi o grupo de controle negativo onde os animais não foram expostos a um anti-helmíntico. Os dados reprodutivos foram coletados no parto, incluindo peso ao nascer, peso da ninhada, tamanho da ninhada e sexo da prole. Os resultados mostraram que não houve diferença estatística (p > 0,05) entre os grupos de tratamento com relação ao peso ao nascer, peso da ninhada, tamanho da ninhada e sexo. No entanto, cutias desparasitadas tiveram maior peso ao nascer (220,24 g vs. 209,1 g) e peso da ninhada (369,8 g vs. 343 g). Os mesmos valores foram obtidos para o tamanho da ninhada (1,7 vs. 1,7) e os animais que foram desparasitados tiveram uma prole feminina maior do que a prole masculina (2,41: 1 vs. 1,11: 1). Esse experimento demonstrou que o uso de anti-helmíntico estrategicamente no manejo de cutias criadas em cativeiro não teve efeito estatístico (p > 0,05) sobre os parâmetros reprodutivos. Portanto, esses animais podem ser mantidos em cativeiro sem serem vermifugados e produzir de forma eficiente com alimentação adequada e manejo do alojamento.

Paternal effects on fetal programming

Paternal programming is the concept that the environmental signals from the sire's experiences leading up to mating can alter semen and ultimately affect the phenotype of resulting offspring. Potential mechanisms carrying the paternal effects to offspring can be associated with epigenetic signatures (DNA methylation, histone modification and non-coding RNAs), oxidative stress, cytokines, and the seminal microbiome. Several opportunities exist for sperm/semen to be influenced during development; these opportunities are within the testicle, the epididymis, or accessory sex glands. Epigenetic signatures of sperm can be impacted during the pre-natal and pre-pubertal periods, during sexual maturity and with advancing sire age. Sperm are susceptible to alterations as dictated by their developmental stage at the time of the perturbation, and sperm and seminal plasma likely have both dependent and independent effects on offspring. Research using rodent models has revealed that many factors including over/under nutrition, dietary fat, protein, and ingredient composition (e.g., macro- or micronutrients), stress, exercise, and exposure to drugs, alcohol, and endocrine disruptors all elicit paternal programming responses that are evident in offspring phenotype. Research using livestock species has also revealed that sire age, fertility level, plane of nutrition, and heat stress can induce alterations in the epigenetic, oxidative stress, cytokine, and microbiome profiles of sperm and/or seminal plasma. In addition, recent findings in pigs, sheep, and cattle have indicated programming effects in blastocysts post-fertilization with some continuing into post-natal life of the offspring. Our research group is focused on understanding the effects of common management scenarios of plane of nutrition and growth rates in bulls and rams on mechanisms resulting in paternal programming and subsequent offspring outcomes. Understanding the implication of paternal programming is imperative as short-term feeding and management decisions have the potential to impact productivity and profitability of our herds for generations to come.(AU)

Efeito da dieta total de vacas leiteiras ao longo da linha de cocho: um estudo de caso / Effects of the total diet of dairy cows along the trough line: a case study / Efectos de la dieta total de vacas lecheras a lo largo de la línea del baldo: un estudio de caso

A pecuária leiteira no Brasil é uma atividade que emprega milhares de pessoas, sendo muito representativa no âmbito do agronegócio, destacando a grande importância do estado de Minas Gerais, consolidado como o maior produtor de leite no país. Destacada a importância dessa atividade, um de seus maiores gargalos está relacionado à nutrição dos animais, visto que ela impacta mais de 50% no custo de produção. Por isso, é essencial que haja um acompanhamento desse setor em específico. Desse modo, o objetivo deste trabalho foi avaliar a distribuição da dieta total misturada na linha de cocho, homogeneização da mistura, carregamento, tempo de mistura e produção de leite. O trabalho realizado foi por meio de um estudo de caso em uma fazenda em Bambuí ­ MG, avaliando quatro lotes em lactação, havendo mudanças das dietas ao longo dos dias de análises. Os resultados variaram nos lotes que não recebiam a dieta no mesmo carregamento, sendo que não houve diferença de carregamento, mistura e água entre os distintos lotes, mas houve diferença na produção de leite com as mudanças das dietas e número de lactação dos animais avaliados. Dessa maneira, o presente trabalho mostra que o acompanhamento diário nas fazendas deve ser realizado com o intuito de minimizar perdas e, assim, haver melhor gestão sobre a atividade.

Dairy farming in Brazil is an activity that employs thousands of people, being very representative in the field of agribusiness, highlighting the great importance of the state of Minas Gerais, consolidated as the largest producer of milk in the country. Highlighting the importance of this activity, one of its biggest bottlenecks is related to animal nutrition, as it impacts more than 50% on production costs. Therefore, it is essential that there is a follow-up of this specific sector. Thus, the objective of this work was to evaluate the distribution of the total diet mixed in the trough line, mixture homogenization, loading, mixing time and milk production. The work carried out was through a case study on a farm in Bambuí - MG, evaluating four lots in lactation, with changes in diets over the days of analysis. The results varied in the lots that did not receive the diet in the come load, and there was no difference in loading, mixture and water between the evaluated lots, but there was a difference in milk production with changes in the diets and number of lactation of the evaluated animals. In this way, the present work shows that the daily monitoring on the farms must be carried out in order to minimize losses and, thus, have more management over the activity.

La ganadería lechera en Brasil es una actividad que emplea a miles de personas, siendo muy representativa en el campo de la agroindustria, destacando la gran importancia del estado de Minas Gerais, consolidado como el mayor productor de leche del país. Resaltando la importancia de esta actividad, uno de sus mayores cuellos de botella está relacionado con la nutrición animal, ya que impacta más del 50% en los costos de producción. Por lo tanto, es fundamental que haya un seguimiento de este sector específico. Así, el objetivo de este trabajo fue evaluar la distribución de la dieta total mezclada en la línea de comederos, homogenización de la mezcla, carga, tiempo de mezclado y producción de leche. El trabajo realizado fue a través de un estudio de caso en una granja en Bambuí - MG, evaluando cuatro lotes en lactancia, con cambios en las dietas a lo largo de los días de análisis. Los resultados variaron en los lotes que no recibieron la dieta en la misma carga, y no hubo diferencia en carga, mezcla y agua entre los lotes evaluados, pero sí hubo diferencia en la producción de leche con cambios en las dietas y número de lactancias de los animales evaluados. De esta forma, el presente trabajo demuestra que se debe realizar el monitoreo diario en las fincas con el fin de minimizar las pérdidas y así tener un mayor manejo sobre la actividad.

Gastrointestinal parasites of wild carnivores from conservation institutions in the Cerrado of Goiás, Brazil / Parasitas gastrintestinais de carnívoros silvestres de instituições de conservação no Cerrado de Goiás, Brasil

Increased interaction between wild and urban environments owing to human population growth, increased anthropization of biomes, and habitat loss for wild animals increases the spread of infectious and parasitic agents. The present study reports on the occurrence of gastrointestinal parasites in carnivorous mammals at two conservation institutions in the state of Goiás, Brazil. Fecal samples from 39 adult carnivores were collected after spontaneous defecation and analyzed by flotation and sedimentation. The structure and management data of each institution were recorded. Parasitism prevalence, binomial confidence intervals (CI) at 95%, variables associated with the presence of contact animals, size of the enclosure and type of food were recorded. The overall prevalence of gastrointestinal parasites in the samples analyzed was 71.8% (CI 55.1­83.0; 28/39). Ancylostomatidae, Toxocara spp., Toxascaris leonina, Strongyloides spp., Calodium hepaticum, and Trematoda eggs, and Cystoisospora spp. oocysts were detected. Environmental conditions were not correlated with parasitism prevalence; however, the parasites found could be managed, considering their biology, such as controlling synanthropic and domestic animals in captivity, feeding with healthy feed.(AU)

A crescente urbanização e a perda de hábitat para animais selvagens tem levado a uma maior interação entre ambientes selvagens e humanos, o que pode favorecer a disseminação de agentes infecciosos e parasitários. O presente estudo relata a ocorrência de parasitas gastrointestinais em mamíferos carnívoros em duas instituições de conservação no estado de Goiás, Brasil. Amostras fecais de 39 carnívoros adultos foram coletadas após a defecação espontânea e analisadas por flotação e sedimentação. Foram registrados os dados de estrutura e gestão de cada instituição. Prevalência de parasitismo, intervalo de confiança binomial (IC) a 95% e variáveis associadas a presença de animais de contato, tamanho do recinto e tipo de alimento foram registrados. A prevalência geral de parasitas gastrointestinais nas amostras analisadas foi de 71,8% (IC 55,1­83,0; 28/39). Foram detectados ovos de Ancylostomatidae, Toxocara spp., Toxascaris leonina, Strongyloides spp., Calodium hepaticum e de Trematoda e oocistos de Cystoisospora spp.. As condições ambientais não apresentaram correlação com a prevalência de parasitismo; entretanto, os parasitos encontrados podem ser manejados, considerando-se sua biologia, como controlar animais sinantrópicos e domésticos em cativeiro, e uso de alimentos livres de contaminantes.(AU)

Retinopatia deficiente de taurina em uma gata / Taurine deficiency retinopathy in a cat

Background: Taurine is an essential amino acid for cats and its deficiency causes an ocular disorder called taurine deficiency retinopathy. The retinal lesion is definitive and can be classified into five progression stages. In an advanced stage, it leads to blindness that in most cases is irreversible. This disease is considered rare as taurine is currently supplemented in commercial cat food. The objective of this report is to describe the ophthalmic changes in a cat with advanced taurine deficiency retinopathy, a rare but current disease that is important for differential diagnosis of blindness in cats. Case: We report the case of an adult mixed-breed cat (weighing 3.4 kg), that was attended by the Ophthalmology Service of the College of Veterinary Medicine and Animal Science (UNESP - Botucatu), which was treated due to complaints of poor visual acuity for about 1 year. The animal, which was previously a stray animal, had been adopted for 2 years and provided premium cat food ad libitum. Ophthalmic examination showed bilateral mydriasis, with negative menace, direct pupillary, and consensual light reflexes. No change was observed in the ocular appendages, cornea, anterior chamber, lens, and vitreous humor. The eyes were normotensive and fluorescein test negative. Direct and indirect fundoscopy revealed an area of ellipsoidal hyperreflexia with darkened margins laterally to the optic nerve disk in the tapetal region and intense retinal vascular attenuation in both eyes, with a diagnosis of taurine deficiency retinopathy. Complete blood count and biochemical analysis parameters were within the normal range, including the leukocyte count. The guardian was instructed to continue feeding the cat balanced cat food and received information on the proper care and management of a blind animal. Discussion: Although taurine deficient retinopathy is currently underdiagnosed due to the supplementation of this amino acid in commercial cat food, animals that are not properly fed, such as those receiving dog food or homemade food, may be deficient in this amino acid. Taurine deficiency and in this case, the consequent taurine deficiency retinopathy, was diagnosed by visualizing the lesion characteristic of this amino acid deficit since no other retinal change presents this aspect in cats. Therefore, this lesion is considered pathognomonic of this deficiency. Hyperreflective retinal lesions with darkened margins indicate the slow chronic progression in the already stabilized lesions. The animal in this report presented pigmented lesion margins, indicating the chronicity of these retinal changes. Moreover, lesion signs are visible on fundoscopy only after a period of 2-11 months of nutritional amino acid deficiency, and complete retinal atrophy usually occurs after at least nine months of taurine deficiency. Thus, complete blindness associated with advanced retinal changes reinforced the suspicion that the animal had taurine deficiency for a prolonged period of time prior to its adoption. Its guardian was instructed to provide balanced commercial cat food, because although retinal lesions are irreversible, cardiac changes resulting from taurine deficiency are reversed with dietary supplementation. In addition, taurine deficiency affects other organs and systems, such as the central nervous, immune, and reproductive systems. In conclusion, although taurine deficiency retinopathy is currently rare, this condition should be considered one of the possible differential diagnoses for blindness in feline patients.

Anthelmintic usage on the Reproductive Parameters in Captive reared Agoutis (Dasyprocta leporina) in Trinidad and Tobago, West Indies / Uso de anti-helmíntico nos parâmetros reprodutivos em cutias criadas em cativeiro (Dasyprocta leporina) em Trinidad e Tobago, Índias Ocidentais

The agouti (Dasyprocta leporina) is a rodent that is found in the Neo-tropical region. This animal is hunted for its meat but has recently been reared in captivity as a source of meat protein in rural communities. A 20-month experiment was carried out to evaluate the effect of an anthelmintic on the reproductive performance of the agouti (Dasyprocta leporina) reared in captivity. This experiment was conducted in the humid tropics of Trinidad and Tobago. Sixteen animals (15 females, 1 male) placed in each of the two treatment groups in a completely randomized study design. In treatment 1 (T1) animals were given subcutaneous injections of Endovet Ces® (Ivermectin/Praziquantel) at 0.2 mg/kg every three months. Treatment 2 (T2) was the negative control group where animals were not exposed to an anthelmintic. Reproductive data were collected at parturition which included birth weight, litter weight, litter size and gender of offspring. The results showed that there was no statistical difference (p > 0.05) between the treatment groups with respect to birth weight, litter weight, litter size and gender. However, agoutis that were dewormed had a higher birth weight (220.24 g vs 209.1 g) and litter weight (369.8 g vs 343 g). The same values were obtained for the litter size (1.7 vs 1.7) and animals that were dewormed had a higher female offspring to male offspring (2.41:1 vs 1.11:1). This experiment demonstrated that the use of an anthelmintic strategically in the management of captive reared agoutis had no statistical effect (p > 0.05) on the reproductive parameters. Therefore, these animals can be kept in captive conditions without being dewormed and produce efficiently with proper feeding and housing management.

A cutia (Dasyprocta leporina) é um roedor que se encontra na região neo-tropical. Esse animal é caçado por sua carne, mas recentemente foi criado em cativeiro como fonte de proteína de carne em comunidades rurais. Um experimento de 20 meses foi realizado para avaliar o efeito de um anti-helmíntico no desempenho reprodutivo de cutias (Dasyprocta leporina) criadas em cativeiro. Esse experimento foi conduzido nos trópicos úmidos de Trinidad e Tobago. Dezesseis animais (15 fêmeas, 1 macho) colocados em cada um dos dois grupos de tratamento em um desenho de estudo completamente randomizado. No tratamento 1 (T1) os animais receberam injeções subcutâneas de Endovet Ces® (Ivermectina / Praziquantel) na dose de 0,2 mg / kg a cada três meses. O tratamento 2 (T2) foi o grupo de controle negativo onde os animais não foram expostos a um anti-helmíntico. Os dados reprodutivos foram coletados no parto, incluindo peso ao nascer, peso da ninhada, tamanho da ninhada e sexo da prole. Os resultados mostraram que não houve diferença estatística (p > 0,05) entre os grupos de tratamento com relação ao peso ao nascer, peso da ninhada, tamanho da ninhada e sexo. No entanto, cutias desparasitadas tiveram maior peso ao nascer (220,24 g vs. 209,1 g) e peso da ninhada (369,8 g vs. 343 g). Os mesmos valores foram obtidos para o tamanho da ninhada (1,7 vs. 1,7) e os animais que foram desparasitados tiveram uma prole feminina maior do que a prole masculina (2,41: 1 vs. 1,11: 1). Esse experimento demonstrou que o uso de anti-helmíntico estrategicamente no manejo de cutias criadas em cativeiro não teve efeito estatístico (p > 0,05) sobre os parâmetros reprodutivos. Portanto, esses animais podem ser mantidos em cativeiro sem serem vermifugados e produzir de forma eficiente com alimentação adequada e manejo do alojamento.

Anthelmintic usage on the Reproductive Parameters in Captive reared Agoutis (Dasyprocta leporina) in Trinidad and Tobago, West Indies / Uso de anti-helmíntico nos parâmetros reprodutivos em cutias criadas em cativeiro (Dasyprocta leporina) em Trinidad e Tobago, Índias Ocidentais

The agouti (Dasyprocta leporina) is a rodent that is found in the Neo-tropical region. This animal is hunted for its meat but has recently been reared in captivity as a source of meat protein in rural communities. A 20-month experiment was carried out to evaluate the effect of an anthelmintic on the reproductive performance of the agouti (Dasyprocta leporina) reared in captivity. This experiment was conducted in the humid tropics of Trinidad and Tobago. Sixteen animals (15 females, 1 male) placed in each of the two treatment groups in a completely randomized study design. In treatment 1 (T1) animals were given subcutaneous injections of Endovet Ces® (Ivermectin/Praziquantel) at 0.2 mg/kg every three months. Treatment 2 (T2) was the negative control group where animals were not exposed to an anthelmintic. Reproductive data were collected at parturition which included birth weight, litter weight, litter size and gender of offspring. The results showed that there was no statistical difference (p > 0.05) between the treatment groups with respect to birth weight, litter weight, litter size and gender. However, agoutis that were dewormed had a higher birth weight (220.24 g vs 209.1 g) and litter weight (369.8 g vs 343 g). The same values were obtained for the litter size (1.7 vs 1.7) and animals that were dewormed had a higher female offspring to male offspring (2.41:1 vs 1.11:1). This experiment demonstrated that the use of an anthelmintic strategically in the management of captive reared agoutis had no statistical effect (p > 0.05) on the reproductive parameters. Therefore, these animals can be kept in captive conditions without being dewormed and produce efficiently with proper feeding and housing management.(AU)

A cutia (Dasyprocta leporina) é um roedor que se encontra na região neo-tropical. Esse animal é caçado por sua carne, mas recentemente foi criado em cativeiro como fonte de proteína de carne em comunidades rurais. Um experimento de 20 meses foi realizado para avaliar o efeito de um anti-helmíntico no desempenho reprodutivo de cutias (Dasyprocta leporina) criadas em cativeiro. Esse experimento foi conduzido nos trópicos úmidos de Trinidad e Tobago. Dezesseis animais (15 fêmeas, 1 macho) colocados em cada um dos dois grupos de tratamento em um desenho de estudo completamente randomizado. No tratamento 1 (T1) os animais receberam injeções subcutâneas de Endovet Ces® (Ivermectina / Praziquantel) na dose de 0,2 mg / kg a cada três meses. O tratamento 2 (T2) foi o grupo de controle negativo onde os animais não foram expostos a um anti-helmíntico. Os dados reprodutivos foram coletados no parto, incluindo peso ao nascer, peso da ninhada, tamanho da ninhada e sexo da prole. Os resultados mostraram que não houve diferença estatística (p > 0,05) entre os grupos de tratamento com relação ao peso ao nascer, peso da ninhada, tamanho da ninhada e sexo. No entanto, cutias desparasitadas tiveram maior peso ao nascer (220,24 g vs. 209,1 g) e peso da ninhada (369,8 g vs. 343 g). Os mesmos valores foram obtidos para o tamanho da ninhada (1,7 vs. 1,7) e os animais que foram desparasitados tiveram uma prole feminina maior do que a prole masculina (2,41: 1 vs. 1,11: 1). Esse experimento demonstrou que o uso de anti-helmíntico estrategicamente no manejo de cutias criadas em cativeiro não teve efeito estatístico (p > 0,05) sobre os parâmetros reprodutivos. Portanto, esses animais podem ser mantidos em cativeiro sem serem vermifugados e produzir de forma eficiente com alimentação adequada e manejo do alojamento.(AU)

Dynamic of fish trophic guilds in the plateau-plain gradient in the Paraguay River, Northern Pantanal

This study aims to evaluate the dynamics of fish trophic guilds according to the longitudinal gradient of the Paraguay River, northern Pantanal, Brazil. Three river segments were sampled: plateau, confluence and plain. These segments have different physical and biological characteristics, with high water flow in forest areas in plateau and slow flow in meanders, with Pantanal typical vegetation. In total, 26,542 individuals distributed in 130 fish species were collected. The sampled species were characterized in seven trophic guilds. From the seven trophic guilds identified, only three were statistically related to the type of the environment; herbivores were more abundant in the plateau, piscivores in the confluence, and invertivores in the plain. According to values of corrected Akaike Information Criteria, the environmental variable that best explains the abundance of piscivorous fishes in the segments sampled in the Paraguay River was water transparency. For herbivores, the model that explained the variation in abundance was composed by temperature, altitude and dense forest proportion. The variable altitude best represented the abundance of invertivores. Water transparency, temperature, altitude, river width and dense forest proportion were determining factors for the distribution of piscivorous, herbivorous and invertivorous fishes as a response to an environmental gradient that meets its ecological requirements. Understanding the trophic relationships is fundamental for management actions, contributing to the maintenance of ecosystem services of different species. Therefore, future research must be taken into account regarding management and ecological relationships.(AU)

Prey selectivity of the invasive largemouth bass towards native and non-native prey: an experimental approach

Non-native predators are known to cause ecological impacts through heightened consumption of resources and decimation of native species. One such species is Micropterus salmoides. Often introduced for sport fishing worldwide; it has been listed by International Union for Conservation of Nature and Natural Resources as one of the 100 of the world's worst invasive species. Whilst impacts conferred towards native prey are well known, its relationship with other non-native species has received much less attention. Therefore, the aim of this study was to experimentally investigate the feeding preference of M. salmoides towards native prey (Geophagus iporangensis) compared to non-native prey (Oreochromis niloticus and Coptodon rendalli) using comparative consumption and the Manly-Chesson selectivity index. We found a higher consumption by M. salmoides towards the non-native prey and a tendency for this non-native preference to increase when prey availability increased. Our results are in line with the hypothesis that the invasion of the non-native predator can be facilitated by the high abundance and reproductive rate of the non-native prey. This is relevant considering the interaction of multiple invaders in an ecosystem, in particular environments modified by humans. Interspecific relationships are complex and their understanding is necessary for environmental management decision-making.(AU)

Predadores não-nativos são conhecidos por causar impactos ecológicos com o aumento do consumo de recursos e do declínio das espécies nativas. Uma dessas espécies é Micropterus salmoides. Frequentemente introduzida ao redor do mundo para pesca esportiva; essa espécie foi listada pela União Internacional para a Conservação da Natureza como uma das 100 piores espécies invasoras do mundo. Embora os impactos nas presas nativas sejam bem conhecidos, sua relação com outras espécies não-nativas tem recebido muito menos atenção. Portanto, o objetivo deste estudo foi pesquisar experimentalmente a preferência alimentar de M. salmoides por presas nativas (Geophagus iporangensis) em comparação com presas não-nativas (Oreochromis niloticus e Coptodon rendalli) comparando o consumo e o índice de seletividade de Manly-Chesson. Encontramos que M. salmoides tem um maior consumo de presas não-nativas e uma tendência de aumento de preferência pela presa não-nativa com o aumento da disponibilidade de presas. Nossos resultados são consistentes com a hipótese de que a invasão do predador não-nativo pode ser facilitada pela grande abundância e capacidade reprodutiva de presas não-nativas. Isso é relevante considerando a interação de múltiplos invasores em um ecossistema, particularmente em ambientes modificados pelo homem. Relações interespecíficas são complexas e seu entendimento é necessário para a tomada de decisões de gestão ambiental.(AU)

Food habits of indian crested porcupine (Hystrix indica) (Kerr 1792), in district Bagh, Azad Jammu and Kashmir / Hábitos alimentares do porco-espinho de crista indiano (Hystrix indica) (Kerr 1972) nos distritos de Bagh, Azad Jammu e Caxemira

The Indian Crested Porcupine (Hystrix indica) is classified as an agricultural pest species. It feeds on plants and crops; hence, it is responsible for massive financial losses worldwide. The current study was conducted to assess the diet composition of Indian Crested Porcupine in District Bagh, Azad Jammu and Kashmir (AJ&K). Thus, fecal samples were collected and examined from different sampling sites. Reference slides of the material collected from the study area were prepared for identification of dietary components in fecal pellets. A total of 80 fecal samples were collected and processed. Percent relative frequencies (P.R.F.) were calculated for each plant species recovered from pellets. Data revealed that Indian Crested Porcupine consumed 31 plant species in its diet, among them Zea mays (34.31±7.76) was the most frequently selected species followed by Rumex obtusifolius (15.32±2.57) and Melia azedarach (12.83±4.79). The study revealed that the greatest diversity of (n=20) plant species were consumed in summer season while minimum (n=13) species were used during winter. Among the parts of plants, stem was highly consumed in spring (57.2%) as compared to seed in fall (36.7%) while spikes and leaf were the least recovered parts from the fecal matter. The Berger-Parker diversity index showed highly diversified food (10.92) in the summer time of the year as compared to the autumn season (2.95). This study provides a baseline for the diet preference of this pest in the study area. Based on current findings, a detailed investigation on damage assessment, exploration, habitat use and management of Indian Crested Porcupine in AJ&K has been recommended.

O porco-espinho de crista indiano (Hystrix indica) é classificado como uma espécie de praga agrícola. Alimenta-se de plantas e colheitas; portanto, é responsável por enormes perdas financeiras em todo o mundo. O estudo atual foi realizado para avaliar a composição da dieta do porco-espinho de crista indiano nos distritos de Bagh, Azad Jammu e Caxemira (AJ&K). Assim, amostras fecais foram coletadas e examinadas em diferentes locais de amostragem. Lâminas de referência do material coletado na área de estudo foram preparadas para identificação dos componentes da dieta em pellets fecais. Um total de 80 amostras fecais foi coletado e processado. As frequências relativas percentuais (P.R.F.) foram calculadas para cada espécie de planta recuperada de pelotas. Os dados revelaram que o porco-espinho indiano consumiu 31 espécies de plantas em sua dieta, entre elas Zea mays (34,31 ± 7,76) foi a espécie mais selecionada, seguida por Rumex obtusifolius (15,32 ± 2,57) e Melia azedarach (12,83 ± 4,79). O estudo revelou que a maior diversidade de (n = 20) espécies de plantas foi consumida no verão, enquanto o mínimo (n = 13) espécies foi utilizado durante o inverno. Entre as partes das plantas, o caule foi muito consumido na primavera (57,2%) em relação à semente no outono (36,7%), enquanto a espiga e a folha foram as partes menos recuperadas da matéria fecal., O índice de diversidade de Berger-Parker mostrou alimentos altamente diversificados (10,92) no verão do ano em comparação com o outono (2,95). Este estudo fornece uma linha de base para a preferência alimentar dessa praga na área de estudo. Com base nas descobertas atuais, uma investigação detalhada sobre avaliação de danos, exploração, uso de habitat e gerenciamento de porco-espinho de crista indiano em AJ&K foi recomendada.