Morphological study of the digestive tract of the cardinal tetra, Paracheirodon axelrodi (Characiformes: Characidae)

The cardinal tetra Paracheirodon axelrodi is a species of the family Characidae of great interest as an ornamental fish. Many aspects of the biology of this species are still unknown. The present work presents a complete description of the different components of the digestive tract of P. axelrodi, analyzing its global anatomical arrangement, the adjacent glands (liver and pancreas) and the previously ignored pyloric caeca, using histological and histochemical techniques with light microscopy and scanning electron microscopy. Three levels of analysis were performed: macroscopic anatomy, histology of the gastrointestinal tract and scanning electron microscopy of mucosal surfaces. The intestinal coefficient (Ci) of P. axelrodi calculated here (0.94) is within the expected range for a carnivorous species. The anatomy of the alimentary and the resulting Ci is consistent with carnivorous diet in line with dietary studies in the wild in this species. The digestive tract, including adjacent glands (liver and pancreas), presented a relatively conserved general pattern within that described for other species of the family Characidae, especially at the histological level.(AU)

El tetra cardenal Paracheirodon axelrodi es una especie de la familia Characidae de gran interés como pez ornamental. Muchos aspectos de la biología de esta especie son aún desconocidos. En este trabajo se presenta una descripción completa de los diferentes componentes del tracto digestivo de P. axelrodi, analizando su disposición anatómica global, las glándulas adyacentes (hígado y páncreas) y la presencia de ciegos pilóricos, mediante técnicas histológicas e histoquímicas con microscopía óptica y electrónica de barrido. Se realizaron tres niveles de análisis: anatomía macroscópica, histología del tracto gastrointestinal y microscopía electrónica de barrido de las superficies mucosas. El coeficiente intestinal (Ci) de P. axelrodi calculado aquí (0,94) está dentro del rango esperado para una especie carnívora. Los estudios anatómicos del tracto alimentario y el Ci son consistentes con una dieta carnívora en línea con los estudios de la dieta en ejemplares silvestres en esta especie. El tracto digestivo, incluyendo las glándulas adyacentes (hígado y páncreas), presentó un patrón general relativamente conservado dentro de lo descrito para otras especies de la familia Characidae, especialmente a nivel histológico.(AU)

Vitality of mycobionts and photobionts after passing through the digestive tract of Constrictotermes cyphergaster (Isoptera) workers / Vitalidade de micobiontes e fotobiontes após passagem pelo trato digestivo de operários de Constrictotermes cyphergaster (Isoptera)

Termites are among the insects that consume lichens and may be potential dispersers of these symbionts. This study evaluated the vitality of photobionts and mycobionts after passing through the digestive tract of Constrictotermes cyphergaster. The percentage of live and dead algae was verified throughout the alimentary canal of 450 workers, originating from five sampled colonies in the Caatinga Dry Forest, NE, Brazil. A progressive growth in algae mortality was observed in the crop, paunch and rectum, however more than 40% of the algae found in faeces presented signs of vitality. Photobiont morphology was different between cells extracted from thallus in natura and algae present in termite faeces. The photobiont cells presented more shrunken cytoplasms after passing through the alimentary canal of C. cyphergaster. There was also an increase between the cell wall space and the cytoplasm membrane of algae found in the termite faecal pellets. Only four broken spores were found in the intestine, which made the vitality analysis unfeasible for these cells. The record of photobiont vitality in termite faecal pellets is indicative of endozoochoric dispersal, suggesting that this relationship between insects and lichens extends beyond a trophic interaction.

Os térmitas estão entre os insetos consumidores de liquens e representam um potencial dispersor desses simbiontes. Este estudo avaliou a vitalidade de fotobiontes e micobiontes depois de terem passado pelo trato digestivo de Constrictotermes cyphergaster. O percentual de algas vivas e mortas foi verificado ao longo do canal alimentar de 450 operários, oriundos de cinco colônias amostradas em Caatinga Dry Forest, NE, Brazil. Um crescimento progressivo na mortalidade das algas foi observado no sentido papo, pança e reto, porém mais de 40% das algas presentes nas fezes apresentaram sinais de vitalidade. A morfologia dos fotobiontes foi diferente entre células extraídas de talos in natura e algas presentes nas fezes dos térmitas. As células do fotobionte apresentaram seu citoplasma mais encolhido, após a passagem pelo canal alimentar de C. cyphergaster. Também houve um aumento entre o espaço da parede celular e a membrana do citoplasma das algas encontradas nas pelotas fecais dos térmitas. Apenas quatro esporos quebrados foram encontrados no intestino dos cupins, o que inviabilizou a análise de vitalidade dessas células. O registro da vitalidade de fotobiontes nas pelotas fecais dos térmitas é um indicativo de dispersão endozoocórica, remetendo que a relação entre esses insetos e os liquens pode ir além de uma interação trófica.

Gastroesophageal tube of the Iguana iguana (Iguanidae): histological description, histochemical and immunohistochemical analysis of 5-HT and SS cells / Tubo gastroesofágico da Iguana iguana (Iguanidae): descrição histológica, histoquímica e análise imunohistoquímica de células 5-HT e SS

Abstract The work aims were to describe the histological and histochemical structure of the gastroesophageal tube of Iguana iguana and verify the occurrence and distribution of immunoreactive serotonin (5-HT) and somatostatin (SS) cells. Fragments of the gastrointestinal tract (GIT) of five iguanas were which underwent standard histological and immunohistochemistry technique. Immunoreactive cells for 5-HT and SS were quantified using the STEPanizer. The oesophagus has ciliated columnar pseudostratified epithelium with staining Alcian blue (AB) + and goblet cells highly reactive to periodic acid Schiff (PAS). In the cervical oesophagus, the numerical density of 5-HT cells per unit area (QA [5-HT cells]/µm2) was 4.6x10-2 ± 2.0 and celomatic oesophagus presented QA = 4.0x10-2 ± 1.0. The epithelium of the stomach is simple columnar, PAS and AB +. The cranial and middle regions of the stomach presented (QA [5-HT cells]/µm2) = 6.18x10-2 ± 3.2 and the caudal region, QA = 0.6x10-2 ± 0.2. The SS cells were only observed in the caudal stomach, with numerical density (QA [SS cells]/µm2) = 1.4x10-2 ± 0.9 In I. iguana, variation was observed in terms of the distribution of mucus secretions and the pattern of occurrence of serotonin and somatostatin-secreting enteroendocrine cells in the TGI, which possibly will result in an interspecific adaptive response.

Resumo Os objetivos do trabalho foram descrever a estrutura histológica e histoquímica do tubo gastroesofágico da Iguana iguana e verificar a ocorrência e distribuição de células serotonina (5-HT) e somatostatina (SS) imunorreativas. Fragmentos do trato gastrointestinal (TGI) de cinco iguanas foram submetidos à técnica histológica e imunohistoquímica padrão. As células imunorreativas para 5-HT e SS foram quantificadas usando o STEPanizer. O esôfago apresenta epitélio pseudoestratificado colunar ciliado Alcian blue (AB) positivo, com células caliciformes altamente reativas ao ácido periódico de Schiff (PAS). No esôfago cervical, a densidade numérica de células 5-HT por unidade de área (QA [células 5-HT] / µm2) foi de 4.6x10-2 ± 2.0 e o esôfago celomático apresentou QA = 4.0x10-2 ± 1.0. O epitélio do estômago é colunar simples, PAS e AB positivo. As regiões cranial e média do estômago apresentaram (QA [células 5-HT] / µm2) = 6.18x10-2 ± 3.2 e a região caudal, QA = 0.6x10-2 ± 0.2. As células SS foram observadas apenas no estômago caudal, com densidade numérica (QA [células SS] / µm2) = 1.4x10-2 ± 0.9. Em I. iguana, foi observada variações em termos da distribuição das secreções de muco e padrão de ocorrência das células enteroendócrinas secretoras de serotonina e somatostatina no TGI, o que possivelmente reflete uma resposta adaptativa interespecifica.

Gastroesophageal foreign bodies in dogs - endoscopy and surgical removal

Background: Gastroesophageal foreign bodies (GFD) are commonly diagnosed in dogs and are considered an endoscopic emergency that, although not resulting in serious clinical sequelae or mortality, can compromise the health and well-being of the patient. The use of the digestive endoscopy for the diagnosis and treatment of GFD can be a valuable and viable alternative. There are cases of GFD in dogs for which the indicated treatment is surgery, which can be performed using minimally invasive or conventional techniques, associated or not with flexible endoscopy. The objective of this work is to describe 16 cases of GFD removal in dogs demonstrating the efficiency of upper digestive endoscopy. Cases: Of the 16 GFD cases, 63% (10/16) were male and 37% (6/16) female. Most aged under 1 year (63%), puppies (5/16) and juveniles (5/16). The patient with the lowest body weight was a miniature pinscher weighing 0.8 kg (Case 14) and the heaviest was an American Pit Bull Terrier weighing 28 kg (Case 11), the mean body weight of patients diagnosed with GFD was 10.2 ± 6.7 kg. Small and medium breeds were more affected, 44.7% (7/16) and 44.7% (7/16), respectively, and large breeds (Golden Retrievier and Bull Terrier), from cases 1 and 4, the least affected, 12.6% (2/16) of the cases. The 16 patients underwent a 12 h food fast and a 4 h water fast, as gastrointestinal emptying in these cases of GFD can be influenced by these foreign bodies. All underwent general inhalation anesthesia with monitoring of physiological parameters (temperature, heart rate, respiratory rate, oxygen saturation and blood pressure) before, during and after EGD, being positioned in left lateral decubitus. The 16 canine patients with suspected GFD underwent EGD for diagnostic confirmation and removal of foreign bodies. Five esophageal FB were diagnosed, 31% (5/16), and 11 gastric FB, 69% (11/16). The most frequently diagnosed foreign bodies were bone and tissue, 37.5% (6/16) and 31% (5/16). Other foreign bodies were materials such as plastics, metals, rubber, foam and stone. Of the 16 cases of GFD, EGD efficiently treated 88% (14/16) without the need for hospitalization, with only supportive treatment for the remission of complications caused by the presence of foreign bodies in the gastroesophageal tract. The main complications related to the presence of GFD were esophagitis in 25% (4/16) of cases, gastritis in 38% (6/16) and both alterations in 13% (2/16). Discussion: In this work, we can observe that more than a third of the clinical cases of treated dogs were diagnosed with GFD, demonstrating that these cases are common in the veterinary clinic. Most of these animals were males less than 1 year old. The improvement of learning in this category can lead these animals to exacerbated oral exploration of new objects. Most FBs were found in the stomach because they were of adequate size, consistency and shape for their passage through the esophagus, whereas esophageal FBs were all bone fragments of rigid consistency with diameters and sizes larger than the esophageal lumen. The interval between the ingestion of the object and the veterinary care can be decisive for the removal of the FB in the esophagus or stomach. Most gastric FBs removed were fabrics and plastics, flexible objects that can pass through the esophageal lumen more easily. Removal of GFD by endoscopy was performed with a high success rate, with only 2 cases being resolved by esophagostomy and gastrotomy. Flexible endoscopy proved to be an efficient technique for removing treated GFD, which can help remove FB during esophagotomy and be associated with rigid endoscopy. Patients recovered quickly and without complications, but it is important to emphasize that inadequate maneuvers and conducts can determine other outcomes. The use of endoscopy for GFD removal needs to be more popularized, as it can ensure better results for dogs treated with GFD.

Cellulolytic activity of anaerobic facultative fungi from the digestive tract of sheep fed with banana leaf hay / Atividade celulolítica de fungos aeróbios facultativos do trato digestório de ovinos alimentados com feno da folha de bananeira

The addition of cellulolytic fungi, or their enzymes, in diets containing high levels of fiber are promising strategy for improving the performance. In this study, the aims were to select cellulolytic fungi from the digestive tract of sheep fed different concentrations of banana leaf hay. Thirty lambs raised in a feedlot were evaluated, distributed in a completely randomized design, with diets containing 0, 125, 250, 375, or 500 g/Kg of dry matter and six replications. Approximately 15 mL of ruminal fluid and swabs from the rectal ampulla were collected. The cultures were carried out in a medium containing microcrystalline cellulose (C medium). The mycelial fungi isolates were identified through the microculture technique. Among the fungi from the ruminal fluid, 23 isolates corresponded to the genus Aspergillus and three to Paecilomyces spp. Among the isolates from the rectal ampulla, seven were A. spp., and three were P. spp. The A. genus predominated among the isolates from both evaluated sites (p <0.05). Fragments of these fungi were inoculated in triplicate in medium C at 37 °C and the cellulolytic activity index (CAI) was determined after 24, 48, and 72 hours of incubation. There was no difference in the CAI of Aspergillus spp. from animals fed different diets or of different evaluated sites (P > 0.05). However, 22 isolates of Aspergillus spp. and three of Paecilomyces spp. showed a CAI > 1, indicating biotechnological potential for cellulase production. These selected isolates could be selected for the elaboration of microbial additives in ruminant diets.(AU)

A adição de fungos celulolíticos, ou suas enzimas, em dietas contendo elevados teores de fibras são estratégias pro-missoras para melhorar o desempenho. Neste estudo os objetivos foram selecionar fungos celulolíticos do trato digestório de ovinos alimentados com diferentes concentrações do feno da folha da bananeira (FBH). Foram avaliados 30 borregos criados em sistema intensivo, distribuídos em delineamento inteiramente ao acaso, com cinco dietas contendo 0, 125, 250, 375 ou 500 g/KG de matéria seca em seis repetições. Foram coletados aproximadamente 15 mL de fluido ruminal e swabs da ampola retal. Os cultivos foram realizados em meio de cultura contendo celulose microcristalina (meio C). Os fungos micelianos foram identificados após a técnica de microcultivo. Entre os fungos provenientes do fluido ruminal, 23 isolados corresponderam ao gênero Aspergillus e três a Paecilomyces spp.. Foram identificados nas fezes dos animais sete Aspergillus spp. e três Paecilomyces spp.. O gênero Aspergillus predominou entre os isolados de ambos os sítios avaliados (p =0,013). Fragmentos desses fungos foram inoculados em triplicada em meio C a 37 °C e determinou-se o índice de atividade celulolítica(IAC) após 24, 48 e 72 horas de incubação. Não houve diferença entre CAI de isolados de Aspergillus spp. provenientes dos animais em diferentes dietas ou sítios avaliados (P > 0.05). Entretanto, 22 isolados de Aspergillus spp. e três de Paecilomyces spp. apresentaram IAC >1, indicando potencial biotecnológico para produção de celulases.(AU)

Gastroesophageal tube of the Iguana iguana (Iguanidae): histological description, histochemical and immunohistochemical analysis of 5-HT and SS cells / Tubo gastroesofágico da Iguana iguana (Iguanidae): descrição histológica, histoquímica e análise imunohistoquímica de células 5-HT e SS

The work aims were to describe the histological and histochemical structure of the gastroesophageal tube of Iguana iguana and verify the occurrence and distribution of immunoreactive serotonin (5-HT) and somatostatin (SS) cells. Fragments of the gastrointestinal tract (GIT) of five iguanas were which underwent standard histological and immunohistochemistry technique. Immunoreactive cells for 5-HT and SS were quantified using the STEPanizer. The oesophagus has ciliated columnar pseudostratified epithelium with staining Alcian blue (AB) + and goblet cells highly reactive to periodic acid Schiff (PAS). In the cervical oesophagus, the numerical density of 5-HT cells per unit area (QA [5-HT cells]/µm2) was 4.6x10-2 ± 2.0 and celomatic oesophagus presented QA = 4.0x10-2 ± 1.0. The epithelium of the stomach is simple columnar, PAS and AB +. The cranial and middle regions of the stomach presented (QA [5-HT cells]/µm2) = 6.18x10-2 ± 3.2 and the caudal region, QA = 0.6x10-2 ± 0.2. The SS cells were only observed in the caudal stomach, with numerical density (QA [SS cells]/µm2) = 1.4x10-2 ± 0.9 In I. iguana, variation was observed in terms of the distribution of mucus secretions and the pattern of occurrence of serotonin and somatostatin-secreting enteroendocrine cells in the TGI, which possibly will result in an interspecific adaptive response.

Os objetivos do trabalho foram descrever a estrutura histológica e histoquímica do tubo gastroesofágico da Iguana iguana e verificar a ocorrência e distribuição de células serotonina (5-HT) e somatostatina (SS) imunorreativas. Fragmentos do trato gastrointestinal (TGI) de cinco iguanas foram submetidos à técnica histológica e imunohistoquímica padrão. As células imunorreativas para 5-HT e SS foram quantificadas usando o STEPanizer. O esôfago apresenta epitélio pseudoestratificado colunar ciliado Alcian blue (AB) positivo, com células caliciformes altamente reativas ao ácido periódico de Schiff (PAS). No esôfago cervical, a densidade numérica de células 5-HT por unidade de área (QA [células 5-HT] / µm2) foi de 4.6x10-2 ± 2.0 e o esôfago celomático apresentou QA = 4.0x10-2 ± 1.0. O epitélio do estômago é colunar simples, PAS e AB positivo. As regiões cranial e média do estômago apresentaram (QA [células 5-HT] / µm2) = 6.18x10-2 ± 3.2 e a região caudal, QA = 0.6x10-2 ± 0.2. As células SS foram observadas apenas no estômago caudal, com densidade numérica (QA [células SS] / µm2) = 1.4x10-2 ± 0.9. Em I. iguana, foi observada variações em termos da distribuição das secreções de muco e padrão de ocorrência das células enteroendócrinas secretoras de serotonina e somatostatina no TGI, o que possivelmente reflete uma resposta adaptativa interespecifica.

Gastroesophageal tube of the Iguana iguana (Iguanidae): histological description, histochemical and immunohistochemical analysis of 5-HT and SS cells / Tubo gastroesofágico da Iguana iguana (Iguanidae): descrição histológica, histoquímica e análise imunohistoquímica de células 5-HT e SS

The work aims were to describe the histological and histochemical structure of the gastroesophageal tube of Iguana iguana and verify the occurrence and distribution of immunoreactive serotonin (5-HT) and somatostatin (SS) cells. Fragments of the gastrointestinal tract (GIT) of five iguanas were which underwent standard histological and immunohistochemistry technique. Immunoreactive cells for 5-HT and SS were quantified using the STEPanizer. The oesophagus has ciliated columnar pseudostratified epithelium with staining Alcian blue (AB) + and goblet cells highly reactive to periodic acid Schiff (PAS). In the cervical oesophagus, the numerical density of 5-HT cells per unit area (QA [5-HT cells]/µm2) was 4.6x10-2 ± 2.0 and celomatic oesophagus presented QA = 4.0x10-2 ± 1.0. The epithelium of the stomach is simple columnar, PAS and AB +. The cranial and middle regions of the stomach presented (QA [5-HT cells]/µm2) = 6.18x10-2 ± 3.2 and the caudal region, QA = 0.6x10-2 ± 0.2. The SS cells were only observed in the caudal stomach, with numerical density (QA [SS cells]/µm2) = 1.4x10-2 ± 0.9 In I. iguana, variation was observed in terms of the distribution of mucus secretions and the pattern of occurrence of serotonin and somatostatin-secreting enteroendocrine cells in the TGI, which possibly will result in an interspecific adaptive response.(AU)

Os objetivos do trabalho foram descrever a estrutura histológica e histoquímica do tubo gastroesofágico da Iguana iguana e verificar a ocorrência e distribuição de células serotonina (5-HT) e somatostatina (SS) imunorreativas. Fragmentos do trato gastrointestinal (TGI) de cinco iguanas foram submetidos à técnica histológica e imunohistoquímica padrão. As células imunorreativas para 5-HT e SS foram quantificadas usando o STEPanizer. O esôfago apresenta epitélio pseudoestratificado colunar ciliado Alcian blue (AB) positivo, com células caliciformes altamente reativas ao ácido periódico de Schiff (PAS). No esôfago cervical, a densidade numérica de células 5-HT por unidade de área (QA [células 5-HT] / µm2) foi de 4.6x10-2 ± 2.0 e o esôfago celomático apresentou QA = 4.0x10-2 ± 1.0. O epitélio do estômago é colunar simples, PAS e AB positivo. As regiões cranial e média do estômago apresentaram (QA [células 5-HT] / µm2) = 6.18x10-2 ± 3.2 e a região caudal, QA = 0.6x10-2 ± 0.2. As células SS foram observadas apenas no estômago caudal, com densidade numérica (QA [células SS] / µm2) = 1.4x10-2 ± 0.9. Em I. iguana, foi observada variações em termos da distribuição das secreções de muco e padrão de ocorrência das células enteroendócrinas secretoras de serotonina e somatostatina no TGI, o que possivelmente reflete uma resposta adaptativa interespecifica.(AU)

Elaboration, characterization, and probiotic viability of synbiotic non-dairy drink based on coconut / Elaboração, caracterização e viabilidade probiótica de bebida simbiótica não-láctea a base de coco

ABSTRACT: Coconut is a fruit grown in more than 80 countries owing to its outstanding nutritional and biological value and it is an important crop for the food industry" por "Coconut is a fruit grown in more than 80 countries and owing to its outstanding nutritional and biological value and it is an important crop for the food industry. Thus, developing new coconut-based products is attractive to explore the benefits provided by microorganisms and improve the nutritional and bioactive composition of coconut products, such as by preparing fermented beverages. This study developed and characterize a drink based on dry coconut with the prebiotic fructooligosaccharide fermented by the probiotic Lactobacillus casei. The drink was formulated, filtered, fermented, matured, and stored under refrigeration (4 °C) for 28 days; it was evaluated for its physical, chemical, antioxidant, and microbiological characteristics. Compared to the standard non-fermented sample during storage, the fermented drink showed significant variations (P < 0.05) in instrumental color, acidity, and pH, while changes in soluble solids and stability index were observed after 7 days of storage. Regarding the chemical composition, all parameters varied significantly after fermentation. The total phenolic compound content and antioxidant capacity increased significantly after fermentation. Significant reductions were observed (P < 0.05) in the viability of Lactobacillus casei after exposure to gastrointestinal tract conditions, with the following counts (in log CFU mL-1) after 0 and 28 days of storage: - initial: 9.23 ± 0.04 and 9.05 ± 0.12; after the gastric phase: 6.21 ± 0.09 and 5.90 ± 0.01; and after the intestinal phase: 4.59 ± 0.33 and 4.75 ± 0.23, respectively.

RESUMO: O coco é uma fruta cultivada em mais de 80 países e devido ao seu excelente valor nutricional e biológico é uma importante cultura para a indústria alimentícia. Assim, desenvolver novos produtos à base de coco é atrativo para explorar os benefícios proporcionados por microrganismos e melhorar a composição nutricional e bioativa de produtos de coco, como na preparação de bebidas fermentadas. Este estudo desenvolveu e caracterizou uma bebida à base de coco seco com o prebiótico frutooligossacarídeo, fermentada pelo probiótico Lactobacillus casei. A bebida foi formulada, filtrada, fermentada, maturada e armazenada sob refrigeração (4 °C) por 28 dias; além de avaliada em relação às suas características físicas, químicas, antioxidantes e microbiológicas. Quando comparada à amostra padrão não-fermentada ao longo do armazenamento, a bebida fermentada apresentou variações significativas (P < 0.05) para cor instrumental, acidez e pH, enquanto as alterações para sólidos solúveis e índice de estabilidade foram observadas a partir do 7° dia de armazenamento. Em relação à composição química, todos os parâmetros variaram significativamente após o processo fermentativo. O teor de compostos fenólicos totais e capacidade antioxidante aumentaram significativamente após a fermentação. Reduções significativas foram observadas (P < 0,05) na viabilidade do Lactobacillus casei após a exposição às condições do trato gastrointestinal, com as seguintes contagens (em UFC mL-1) após 0 e 28 dias de armazenamento: - inicial: 9,23 ± 0.04 e 9,05 ± 0,12; após a fase gástrica: 6,21 ± 0,09 e 5,90 ± 0,01; e após a fase intestinal: 4,59 ± 0,33 e 4,75 ± 0,23, respectivamente.

Elaboration, characterization, and probiotic viability of synbiotic non-dairy drink based on coconut / Elaboração, caracterização e viabilidade probiótica de bebida simbiótica não-láctea a base de coco

Coconut is a fruit grown in more than 80 countries owing to its outstanding nutritional and biological value and it is an important crop for the food industry" por "Coconut is a fruit grown in more than 80 countries and owing to its outstanding nutritional and biological value and it is an important crop for the food industry. Thus, developing new coconut-based products is attractive to explore the benefits provided by microorganisms and improve the nutritional and bioactive composition of coconut products, such as by preparing fermented beverages. This study developed and characterize a drink based on dry coconut with the prebiotic fructooligosaccharide fermented by the probiotic Lactobacillus casei. The drink was formulated, filtered, fermented, matured, and stored under refrigeration (4 °C) for 28 days; it was evaluated for its physical, chemical, antioxidant, and microbiological characteristics. Compared to the standard non-fermented sample during storage, the fermented drink showed significant variations (P < 0.05) in instrumental color, acidity, and pH, while changes in soluble solids and stability index were observed after 7 days of storage. Regarding the chemical composition, all parameters varied significantly after fermentation. The total phenolic compound content and antioxidant capacity increased significantly after fermentation. Significant reductions were observed (P < 0.05) in the viability of Lactobacillus casei after exposure to gastrointestinal tract conditions, with the following counts (in log CFU mL-1) after 0 and 28 days of storage: - initial: 9.23 ± 0.04 and 9.05 ± 0.12; after the gastric phase: 6.21 ± 0.09 and 5.90 ± 0.01; and after the intestinal phase: 4.59 ± 0.33 and 4.75 ± 0.23, respectively.

O coco é uma fruta cultivada em mais de 80 países e devido ao seu excelente valor nutricional e biológico é uma importante cultura para a indústria alimentícia. Assim, desenvolver novos produtos à base de coco é atrativo para explorar os benefícios proporcionados por microrganismos e melhorar a composição nutricional e bioativa de produtos de coco, como na preparação de bebidas fermentadas. Este estudo desenvolveu e caracterizou uma bebida à base de coco seco com o prebiótico frutooligossacarídeo, fermentada pelo probiótico Lactobacillus casei. A bebida foi formulada, filtrada, fermentada, maturada e armazenada sob refrigeração (4 °C) por 28 dias; além de avaliada em relação às suas características físicas, químicas, antioxidantes e microbiológicas. Quando comparada à amostra padrão não-fermentada ao longo do armazenamento, a bebida fermentada apresentou variações significativas (P < 0.05) para cor instrumental, acidez e pH, enquanto as alterações para sólidos solúveis e índice de estabilidade foram observadas a partir do 7° dia de armazenamento. Em relação à composição química, todos os parâmetros variaram significativamente após o processo fermentativo. O teor de compostos fenólicos totais e capacidade antioxidante aumentaram significativamente após a fermentação. Reduções significativas foram observadas (P < 0,05) na viabilidade do Lactobacillus casei após a exposição às condições do trato gastrointestinal, com as seguintes contagens (em UFC mL-1) após 0 e 28 dias de armazenamento: - inicial: 9,23 ± 0.04 e 9,05 ± 0,12; após a fase gástrica: 6,21 ± 0,09 e 5,90 ± 0,01; e após a fase intestinal: 4,59 ± 0,33 e 4,75 ± 0,23, respectivamente.

Emulsifier in Diets with Different Alternative Lipid Sources: Effects on the Health of Japanese Quails

The present research aims to evaluate the inclusion of an emulsifying additive based on mono- and diglycerides of fatty acids in diets with different lipid sources, studying its effects on duodenum histology, biometry of the digestive and reproductive tract, and blood biochemical profile of Japanese quails. The study was carried out in the experimental aviary of the Federal University of Grande Dourados, Mato Grosso do Sul, Brazil. The experimental design was completely randomized, with diets in a 3x2 factorial design with three different lipid sources (soybean oil, poultry fat, and beef tallow), either supplemented or not with an emulsifier. The birds that received a diet composed of poultry fat and an emulsifying additive had a larger gizzard. Animals that received a diet containing an emulsifier, regardless of the lipid source, had greater heart weight. The duodenum of animals that received bovine tallow in their diet showed a greater height and villus:crypt ratio. Biochemical parameters were not affected by either the lipid source or the inclusion of emulsifier in quail diets. The biochemical blood profile of birds in treatments did not show significant changes among all diets used. The use of soybean oil, bovine tallow, and poultry fat with or without the inclusion of an emulsifier does not show changes in the health and development of the quails' organs, which also indicates that the health of Japanese quails in the laying phase is kept in good standards.(AU)

Addition of calcitic seaweed in the diet of sows positively affects the number of live-born piglets and milk parameters

This study was conducted to assess the effects of maternal dietary calcitic seaweed (CSW) on performance and blood metabolites of sows, and on performance, blood metabolites, intestinal microbiota, and parameters of gastrointestinal tract and bone of litters. On d 21 (post-insemination), non-pregnant sows were removed from the trial, remaining 19 sows in control group (without CSW) and 16 sows receiving CSW. Then, a total of 35 sows were allocated in a randomized block design with two treatments: control diet with calcitic limestone plus dicalcium phosphate (CTL) or CTL plus 0.4% CSW. In gestation, sows were fed twice a day (07:00 and 15:00 h) to reach an intake of 2.5 kg animal−1 day−1 divided into two equal meals. On parturition day, sows were offered only 0.5 kg feed animal−1. Throughout lactation, sows were fed three times a day (≅7 kg animal−1 day−1). All diets were provided as mash. Results suggested that sows fed CTL had litters with lower body weight at birth compared with those fed CSW. Sows fed CSW had 14.28% more live-born piglets and lower stillborns. Piglets from sows fed CSW showed greater calcium concentration on d 14 after birth than those from sows fed CTL. Sows fed CSW showed better milk chemical composition and an increase of 27.16% in milk production compared with those fed CTL. Piglets from sows fed CSW had an increase in cecum content in the Enterobacteriaceae count. This study showed that adding 0.4% CSW in the diet of pregnant and lactating sows as an organic calcium source positively influences the number of live-born piglets and the percentage of stillborns. In addition, milk composition and production are also improved without affecting piglets' biological response.(AU)

Trophic relationships among three species of ornamental fish from the region of Lake Amanã, Amazon / Relações tróficas entre três espécies de peixes ornamentais da região do lago de Amanã, Amazonas

The present study objectified to evaluate the trophic relationships and the possible aggressive mimicry involving Carnegiella strigata, Carnegiella marthae and Gnathocharax steindachneri, in lowland forest streams. Samplings occurred in November 2002, March and August 2003 in Amanã Sustainable Development Reserve (ASDR). The total of 943 fish was caught, being 79.43% of C. marthae, 3.18% of C. strigata and 17.39% of G. steindachneri. Stomach contents, degree of stomach repletion, relative volume and frequency of occurrence of food items were evaluated. Thirteen food items were identified, and the presence of terrestrial and aquatic insects indicates the high dependence of these species and the flooded forest. Low feeding overlap was observed between Carnegiella strigata and C. marthae compared to Gnathocharax steindachneri. However, the overlap between the two Carnegiella species was relatively high, which could explain the low frequency of syntopic occurrence among these species, suggesting a possible case of competitive exclusion.

Este estudo teve como objetivo analisar as relações tróficas e a possível existência de uma relação de mimetismo agressivo envolvendo Carnegiella strigata, Carnegiella marthae e Gnathocharax steindachneri, em igarapés de terra firme. As coletas ocorreram em novembro de 2002, março e agosto de 2003 e foram realizadas na Reserva de Desenvolvimento Sustentável de Amanã (RDSA). Foram capturados 943 peixes, onde C. marthae representou 79,43%, C. strigata 3,18% e G. steindachneri 17,39%. Foram analisados os conteúdos estomacais e verificados grau de repleção, volume relativo e frequência de ocorrência dos alimentos. Foram identificados 13 itens alimentares, onde insetos terrestres e aquáticos denotaram alto grau de dependência destas espécies em relação à floresta alagada. Ocorreu baixa sobreposição alimentar das espécies Carnegiella strigata e C. marthae em relação a Gnathocharax steindachneri. Entretanto, a sobreposição entre as duas espécies de Carnegiella foi relativamente alta, o que poderia explicar a baixa frequência de ocorrência sintópica entre essas espécies, sugerindo um possível caso de exclusão competitiva.

Performance, gastrointestinal morphometry, carcass and non-carcass traits in sheep finished on diets containing canola (Brassica napusL. )

The objective of this study was to evaluate performance, gastrointestinal morphometry, carcass,and non-carcass traits in lambs finished on different levels of canola grain in thediet. Twenty-seven Santa Ines lambs with an average initial weight of 19.33 ± 1.39 kg were given different levels of canola grain in the diet: 0, 8,and 16%. Weights and body condition scores were determined at the beginning, every 14 days and at the end of the experimental period. Animals were slaughtered to assess gastrointestinal morphometry, carcass and non-carcass traits. The statistical design used was completely randomized. The inclusion of canola grain showed a linear effect (0.723) for the width and height of ruminal papillae in the ventral region of the rumen. Intestinal villi and crypts showed a quadratic effect, with peaks of 0.62&956;m and 0.43&956;m, with the inclusion of 8 and 16%, respectively. When evaluating carcass traits, a decreasing linear effect was found for hot and cold carcass yield with 16% inclusion of canola. Canola grain can be used as an alternative in diets for finishing sheep up to 8% inclusion in the diet without affecting performance, ruminal and intestinal histometry, carcass,and non-carcass traits.(AU)

Digestive system of Anourosorex squamipes - appearance and morphological features

Background: Anourosorex squamipes have a wide range of feeding habits, which is a kind of omnivorous animal. As an indispensable part of organisms, the differentiation degree of digestive system indicates the evolution degree of species. The higher the evolution degree of animals, the higher the differentiation of digestive system. At present, the research on Anourosorex squamipes digestive system mainly focuses on its feeding habits and mainly depends on the direct observation of gastric contents with the naked eye, but the research on the morphology of digestive system has not been reported. Materials, Methods & Results: Twenty adult Anourosorex squamipes were selected to investigate the characteristics of the appearance, including the contour parameters (body weight, body length, forefoot length, posterior foot length, nasal length, tail length), digestive system parameters (length of esophagus, intestinal length, intestinal weight, stomach weight and liver weight) and morphological structure of the small intestine by histological methods. The results showed that there were no significant differences in body weight, body length, forefoot length, posterior foot length, nasal length and tail length between males and females in Anourosorex squamipes, and no significant differences were observed in parameters of digestive system in length of esophagus, intestinal length, intestinal weight, stomach weight and liver weight. There were no significant differences in villus height, muscular layer thickness and villus height/recess depth (V/C) between males and females, but only ileal crypt depth decreased significantly in males. Discussion: Animal morphological characteristics are closely related to their life habits, unique organs and lifestyles ensure that they thrive on earth. In our study, we found that unique characters are found by observing the appearance of Anourosorex squamipes, the snout is blunt and short, the eyes are degenerated, and the tail is very short and covered with scales, which may be related to its feeding habits, the living environment-long-term nocturnal life in underground with minimal exposure to sunlight, and the unique tail can reduce the friction between tail and soil during the movement. There are certain differences in the appearance parameters of male and female Anourosorex squamipes, but are not significant. The esophagus, stomach, intestine, liver and other parameters of Anourosorex squamipes have little difference between males and females, indicating that Anourosorex squamipes don't have obvious distinctions between males and females in food intake. However, the liver weight of male Anourosorex squamipes is larger than female, indicating that the physiological metabolic capacity of male is larger than female. It is speculated that this may be related to the difference in the amount of activity between males and females in daily life. Males are more active and require more energy, but their digestion and absorption abilities are weaker than females. Therefore, the male Anourosorex squamipes needs more food to provide energy to meet the daily physiological metabolism, and the larger stomach capacity provides the essential conditions for the male to store more food. In addition, we found that the crypt depth of male Anourosorex squamipes is generally greater than that of females, especially in the ileal crypt depth, indicating that the female's cell maturation rate is greater than that of the male. It is speculated that the female has a greater demand for nutrient absorption and better digestion and absorption of food, because females need to give birth to offspring and require greater nutrition.

Inhibitory effect of different probiotic formulations on Salmonella Heidelberg, avian pathogenic Escherichia coli and Campylobacter jejuni

A wide variety of microorganism species have been used as probiotics to improve the intestinal microbial balance, control and prevent the colonization of pathogenic bacteria and promote growth in animal production. Based on the various beneficial factors of probiotic agents, this study aimed to evaluate the effectiveness of three different formulations of probiotics strains through the analysis of antagonistic capacity against common pathogens from chickens gastrointestinal tract. Three formulations composed by Bacillus, Lactobacillus, Saccharomyces, Enterococcus, Bifidobacterium and Pediococcus were tested in vitro by the method of probiotic culture spot in plates seeded with inocula of Escherichia coli, Salmonella Heidelberg and Campylobacter jejuni. The inhibition halos were measured and classified according to the degree of pathogenic bacteria inhibition. The formulation containing an association among Bacillus, Lactobacillus and Saccharomyces presented better results when compared to the other formulations.

Primary intestinal fibrosarcoma in cats

Background: Fibrosarcomas are malignant neoplasms of mesenchymal origin and can have different symptoms depending on the species, age, location and etiopathogenesis. Intestinal tumors in domestic cats are common and the small intestine is the most common site; however, fibrosarcomas are rare in the intestine of all animal species. This work reports intestinal fibrosarcoma in 2 domestic cats and aims to clarify and present information concerning this neoplastic type in the gastrointestinal tract of this species. Cases: We report 2 cases of intestinal fibrosarcoma in domestic felines (Felis catus). Cat 1. A 14-year-old female Persian breed, domestic cat, was taken to the Feline Sector of the Veterinary Hospital of Small Animals (HVPA) of the Federal Rural University of Rio de Janeiro (UFRRJ). The main complaint was chronic constipation and rectal prolapse. The clinical examination revealed an ulcerated mass, measuring 4.0 cm x 1.7 cm. Cat 2. A 10-year-old female undefined breed, domestic cat, was taken to the private clinic. The main complaint was diarrhea with bloody and rectal prolapse. The clinical examination revealed nodule measuring 2.5 cm in diameter. The surgical option decided upon was to use the rectal pull-through technique in both animals. The patients had no trans-surgical or postoperative complications. The material collected during the surgical interventions was analyzed macroscopically and fixed in 10% buffered formalin for 24 h and then sent to the Histopathology Laboratory of the Pathological Anatomy Sector (SAP) at UFRRJ for the cat 1 and in private laboratory for the cat 2. After fixation, it was cleaved for routine microscope exam using Hematoxylin and Eosin (HE) stains and for the histochemical method of Masson's Trichrome staining technique. Complementary immunohistochemistry tests and electron microscopy were also performed. The patients were followed up clinically, showing complete remission of the clinical signs and survival for approximately 1 year after the neoplastic resection. Discussion: There are few reports of intestinal fibrosarcomas in veterinary medicine, therefore, little is known about racial predilection, age, sex or biological behavior. As far as these authors know, this is the 6th and 7th report of this neoplasm with a primary site in the large intestine in this species. The morphological diagnosis of fibrosarcoma is relatively simple, whereas, in some cases the differential diagnosis for tumors of the peripheral nerve sheath, leiomyosarcomas and gastrointestinal stromal tumor (GIST) can be extremely difficult. The immunohistochemistry technique in these cases may not be particularly useful. The fibrosarcoma diagnosis was also confirmed by electron microscopy since no evidence was found that could lead to a neuronal origin, thus excluding tumors such as neurofibrosarcoma and schawnoma, corroborating the immunohistochemical examination. The surgical management of tumor resection with wide safety margins (minimum 2 cm) remains the "gold standard" therapy for dealing with fibrosarcomas since they have a low response rate to chemotherapy and radiotherapy and the use of these therapies as an adjuvant is controversial. The advantages of this technique are related to the surgical time, simplicity, easy access and reduction in the risk of abdominal contamination. Histopathological, immunohistochemistry and electron microscopy evaluations were sufficient to enable the diagnosis of an intestinal fibrosarcoma in both cats. The occurrence of this neoplasm with intestinal involvement in the feline species is rare; therefore, this description is important as it provides information about epidemiology, associated signs, differential diagnoses, biological behavior, treatment and prognosis.

Niche partitioning of two piscivorous fish species in a river in the western brazilian amazon / Particionamento de nicho de duas espécies de peixes piscívoros em um rio na amazônia ocidental brasileira

We analyzed the seasonal variation in the diet, trophic niche breadth (Levins index), the partitioning of food resources (Pianka's symmetric index) and trophic level (weighed average of trophic level of each prey determined in FishBase and SeaLifeBase platform) of Plagioscion squamosissimus (Heckel, 1840) and Hydrolycus scomberoides (Cuvier, 1819) in the Machado River, Rondônia, Brazil. Fish samplings were conducted bimonthly from June 2013 to May 2015 in five sites, using eight sets of gillnets. The occurrence frequency and volumetric frequency were used to quantify the food items. We analyzed the stomach contents of 283 individuals, 134 of H. scomberoides and 149 of P. squamosissimus. Fish were the most consumed food item by both piscivorous species. However, H. scomberoides mostly ingested pelagic fish (e.g. Characiformes fishes and Prochilodus nigricas Spix & Agassiz, 1829), while P. squamosissimus mostly consumed benthic fish [e.g. Pimelodus blochii Valenciennes, 1840 and Tenellus trimaculatus (Boulenger, 1898)]. Hydrolycus scomberoides presented the trophic level 3.55 for both periods analyzed, while P. squamosissimus 4.01 in the flood period and 3.82 in the drought period. Seasonal variations in the diet of H. scomberoides and P. squamosissimus were observed (PERMANOVA). Specifically, P. squamosissimus consumed mainly "Siluriformes" fishes and P. blochii in the drought period. The trophic niche breadth of P. squamosissimus was greater than that of H. scomberoides in the flood period. The species P. squamosissimus and H. scomberoides had low (0.35) food niche overlap in both seasons analysed. The data indicated that P. squamosissimus has a generalist feeding habit, while H. scomberoides is specialized in prey selection. The overlap of food niche between the species in both periods of the hydrological cycle was low, indicating that niche partitioning was probably the main mechanism of coexistence of these species, with little relationship with variations of the hydrological cycle.

Nós analisamos a variação sazonal na dieta, a amplitude do nicho trófico (Índice de Levins), a partição dos recursos alimentares (Índice simétrico de Pianka) e o nível trófico (média do nível trófico de cada presa determinada pela plataforma FishBase e SeaLifeBase) de Plagioscion squamosissimus (Heckel, 1840) e Hydrolycus scomberoides (Cuvier, 1819) no rio Machado, Rondônia, Brasil. As amostragens de peixes foram realizadas bimestralmente de junho de 2013 a maio de 2015 em cinco locais, utilizando oito conjuntos de redes de emalhar. A frequência de ocorrência e a frequência volumétrica foram utilizados para quantificar os itens alimentares. Analisamos o conteúdo estomacal de 283 indivíduos, 134 de H. scomberoides e 149 de P. squamosissimus. Peixes foram o item alimentar mais consumido pelas duas espécies piscívoras. No entanto, H. scomberoides ingeriu principalmente peixes pelágicos (ex. peixes Characiformes e Prochilodus nigricas Spix & Agassiz, 1829), enquanto P. squamosissimus consumiu principalmente peixes bentônicos [ex. Pimelodus blochii Valenciennes, 1840 e Tenellus trimaculatus (Boulenger, 1898)]. Hydrolycus scomberoides apresentou nível trófico de 3,55 para ambos os períodos analisados, enquanto para P. squamosissimus o nível trófico foi de 4,01 no período da cheia e 3,82 no período de seca. Variações sazonais na dieta de H. scomberoides e P. squamosissimus foram observadas (PERMANOVA). Especificamente, P. squamosissimus consumiu principalmente peixes "Siluriformes" e P. blochii no período de seca. A largura do nicho trófico de P. squamosissimus foi maior que a de H. scomberoides no período da cheia. Plagioscion squamosissimus e H. scomberoides apresentaram baixa (0,35) sobreposição de nicho alimentar nas duas estações analisadas. Os dados indicaram que P. squamosissimus tem hábito alimentar generalista, enquanto H. scomberoides é especializado na seleção de presas. A sobreposição de nicho alimentar entre as espécies em ambos os períodos do ciclo hidrológico foi baixa, indicando que a partição de nicho foi provavelmente o principal mecanismo de coexistência dessas espécies, com pouca relação com as variações do ciclo hidrológico.

Ultrastructural study of the esophagus and stomach of Arapaima gigas (Schinz 1822), juvenile paiche, created excavated tank

Paiche (Arapaima gigas) belongs to the Kingdom Animalia, Phylum Chordata, Class Actinopterygii, Order Osteoglossiformes, Family Arapaimidae, Genus Arapaima, and its origin may date to the Jurassic period. The species has natural habitat in the Amazonian rivers, found mainly in marginal lakes, being considered an important fishing resource, with high market value and high demand for meat and leather in both Brazilian and international trade. This study aims to report the morphology of the esophagus and stomach by light microscopy and scanning electronics microscopy. The esophagus was presented as muscular, short, tubular and fan-shaped in the cranial portion, also presenting deep longitudinal folds, and the entire mucosa is covered by mucus secretory cells with distinct morphological characteristics. Pirarurcu's stomach has a J-shape divided into three regions: cardiac with a lighter aspect, fundus portion with few folds in the mucosa, and pyloric with deeper folds, also presenting gastroliths in fundus and pyloric portions. Both microscopy studies highlighted three glandular regions, composed by mucoid columnar epithelial cells, gastric crypts with different shapes and sizes depending on each portion, in which the different shapes of the mucosal folds in each region of the stomach were evident, and digitiform microsaliences were found in the cardiac region, and micro-orifices and desmosome in the fundus region. Also, fundus and pyloric portions produce more mucus than the cardiac. Then morphology found was consistent with the eating habits and management of distinct characteristics of the digestive tract.(AU)

Prevalência de helmintos gastrintestinais em equinos no extremo sul do Rio Grande do Sul / Prevalence of gastrointestinal helminths in horses in the extreme south of Rio Grande do Sul

Objetivou-se através deste estudo determinar a prevalência de helmintos gastrintestinais em equinos do extremo sul do estado do Rio Grande do Sul, Brasil. Para a realização deste trabalho, foram utilizados resultados de exames coproparasitologicos de amostras analisadas no Laboratório de Doenças Parasitárias (LADOPAR) da Faculdade de Veterinária da Universidade Federal de Pelotas entre os anos de 2014 e 2016. Neste período, foram analisadas 972 amostras fecais de equinos. Das amostras analisadas, 80,5% apresentaram-se positivas para algum gênero de parasito gastrintestinal. Além disso, os ovos da família Strongylidae foram os mais prevalentes, sendo observados em 78,1% das amostras. Desta forma, conclui-se que a maioria dos animais avaliados estavam parasitados por algum gênero de helminto gastrintestinal, indicando a presença destes agentes nos equinos criados na região sul do estado.

The objective of this study was to determine the prevalence of gastrointestinal helminths in horses from the extreme south of the state of Rio Grande do Sul, Brazil. To carry out this work, results of coproparasitological examinations of samples analyzed at the Laboratory of Parasitic Diseases (LADOPAR) of the Veterinary Faculty of the Federal University of Pelotas between 2014 and 2016 were used. During this period, 972 fecal samples of horses were analyzed. Among the analyzed samples, 80.5% were positive for some genus of gastrointestinal parasite. Moreover, the eggs of the family Strongylidae were the most prevalent, being observed in 78.1% of the samples. In conclusion, most of the animals evaluated were parasitized by some gastrointestinal helminth genus, indicating the presence of these agents in horses raised in the southern region of the state.

Diseases of the digestive system of agoutis (Dasyprocta leporina) raised in captivity in the Brazilian semiarid region / Doenças do sistema digestivo de cutias (Dasyprocta leporina) criadas em cativeiro na região semiárida brasileira

The objective of this study was to describe the clinical and pathological aspects of diseases of the digestive system in agoutis (Dasyprocta leporina Linnaeus, 1758) diagnosed by the "Laboratório de Patologia Veterinária" (Veterinary Pathology Laboratory) of the "Universidade Federal Rural do Semi-Árido" (UFERSA), from January 2018 to February 2020. During the study period, necropsy and a survey of the clinical history of 27 agoutis were performed, 25.93% (7/27) of which were diagnosed with digestive system diseases. The percentages of digestive tract diseases among the diagnosed were: acute carbohydrate overload (11.12%), gastric ulcer (7.41%), gastric volvulus (3.70%), and intestinal volvulus (3.70%). Studies on the occurrence rate of these diseases, as well as the description of their clinical and anatomopathological aspects, may serve as a basis for guiding the appropriate management in the breeding of these animals.

O objetivo deste estudo foi descrever os aspectos clínicos e patológicos das doenças do aparelho digestivo em cutias (Dasyprocta leporina Linnaeus, 1758) diagnosticadas pelo Laboratório de Patologia Veterinária da Universidade Federal Rural do Semiárido (UFERSA), de janeiro 2018 a fevereiro de 2020. Durante o período do estudo, foram realizadas necropsias e levantamento da história clínica de 27 cutias, sendo 25,93% (7/27) diagnosticadas com doenças do aparelho digestivo. Os percentuais de doenças do aparelho digestivo foram: sobrecarga aguda de carboidratos (11,12%), úlcera gástrica (7,41%), vólvulo gástrico (3,70%) e vólvulo intestinal (3,70%). Estudos sobre a taxa de ocorrência dessas doenças, bem como a descrição de seus aspectos clínicos e anatomopatológicos, podem servir de base para orientar o manejo adequado na criação dessa espécie.