First report of nocardiosis due to Nocardia asteroides in a domestic canary (Serinus canaria domestica) in Mexico

Nocardiosis is a multi-systemic disease that has been reported in several species of birds. It is characterized by the development of granulomatous inflammation in several organs, mainly affecting the respiratory system. An adult male domestic canary (Serinus canaria domestica) weighing 14.5 g was received for examination. Multiple nodules were identified in the epicardium, air sacs, and lungs. Histologically these nodules corresponded to granulomas, in which numerous thin, filamentous branching bacteria were identified. Gram stains of the epicardium, air sacs, and lungs revealed many filamentous branching Gram-positive bacteria. These bacteria were also strongly acid-fast positive by Fite-Faraco stain. PCR end-point analysis and sequencing using total DNA extracted from formalin-fixed paraffin-embedded samples of lungs, heart, and air sacs confirmed the presence of Nocardia asteroides. To the best of the authors' knowledge, this is the first case report of nocardiosis associated with Nocardia asteroides in a Domestic Canary (Serinus canaria domestica) in Mexico.(AU)

Nocardiose em gatos - achados clínicos, anatomopatológicos e morfotintoriais / Nocardiosis in cats - clinical, anatomopathological and morphotintorial characteristics

Background: Nocardiosis is an infectious bacterial disease that can cause cutaneous/ subcutaneous, pulmonary and systemic lesions in different species of domestic animals. The type of transmission occurs through mechanical lesions on the skin or contamination of wounds, in cases of skin involvement, inhalation of aerosols and ingestion of contaminated materials are involved in the pathogenesis of the respiratory and digestive form of the disease. This paper described 4 cases of nocardiosis in cats, addressing the clinical, anatomopathological and morphotintorial characteristics of Nocardia sp. Cases: Four cases of nocardiosis in cats were reviewed, in which data related to breed, sex, age, origin, clinical signs, macroscopic and histological lesions described in necropsy protocols were evaluated. The histological tissue sections stained with Hematoxylin and Eosin (HE) were evaluated in order to characterize the inflammatory response in each case. In addition, paraffin blocks of fragments from affected organ were selected to perform special histochemical staining techniques of Grocott Methenamine Silver (GMS), modified Ziehl-Neelsen, Gram Brown-Brenn and Giemsa stain which are the most characterized techniques used for histopathological diagnoses and it was also used an immunohistochemical test with polyclonal antibody anti-Nocardia sp. (non-commercial). The animals were adults of both sexes, mixed breed, not castrated and semi-domesticated. Neither immunosuppressive factors nor concomitant diseases were identified in the cases studied. The main clinical signs were apathy, anorexia, dehydration, phlegmon and draining tracts. Macroscopically, skin / subcutaneous tissue (3/4), skeletal muscle (2/4), lymph nodes (2/4), liver (2/4), omentum (1/4), spleen (1/4) were affected. In addition, it could be noted that mandibular bone (1/4), pleural tissue (1/4), left testicle (1/4) and Central Nervous System (CNS) (1/4) were also affected by this disease. Microscopically, regarding all cases, there was a pyogranulomatous inflammation in the affected organs. With respect to cases 1, 3 and 4, filamentous, branched, slightly basophilic structures in loose or individual aggregates in the interior of the pseudo-rosettes and in the necrotic areas were observed in the HEstained tissue sections. In all cases submitted to special histochemical techniques, filamentous, branched, individual or loose aggregate structures were observed, the samples were impregnated with silver, and bacteria appear as blue using the Brown-Brenn Gram technique, and stained red in the modified Ziehl-Neelsen, and stained faintly pink in Giemsa stain. The bacteria were observed mainly in the border of the pyogranulomas, in the center of the pseudo-rosettes and in the necrotic areas, being compatible with the infection by Nocardia sp. All cases were positive for immunohistochemistry (IHC). Discussion: Nocardiosis was diagnosed in all cats in this study based on the anatomopathological findings associated with the visualization of the agent and its morphotintorial characteristics by using special histochemical stains and being confirmed by IHC. It occurs mainly in the cutaneous and/or subcutaneous tissues, with systemic involvement and death of the affected animals, in addition to affecting bone tissue considered an uncommon site for the disease. The diagnosis can be established based on the anatomopathological findings associated with the morphotintorial characteristics by using special histochemical stains, which are important for evidencing and morphologically characterizing the agent, as well as being confirmed by IHC.

Bovine mastitis caused by multidrug-resistant Nocardia farcinica

Background: Mastitis caused by Nocardia is characterized by pyogranulomatous inflammation related to inadequatehygiene conditions and is difficult to treat. Prompted by the absence of documentation of Nocardia farcinica associated tobovine mastitis in the Northeast region of Brazil, this is the first report to describe bovine mastitis caused by multidrugresistant N. farcinica.Case: Four milk samples (one from each teat) obtained from a 3-year-old Jersey cow raised on a property located in the metropolitan region of Recife, Pernambuco state, Brazil, were submitted to the Laboratory of Infectious-Contagious Diseases ofthe Veterinary Hospital at Universidade Federal Rural de Pernambuco [UFRPE]. At the laboratory, samples were cultured inbase agar enriched with 7% sheep blood (blood agar) in a microbiological incubator at 37°C under aerobic conditions for 72h. After only 48 h, however, pure bacterial colony growth was observed in all samples. Macroscopic analysis revealed smallcolonies, with an irregular shape, dry aspect, and greyish in color. Gram-positive rods forming filaments and/or ramificationswere observed using a Gram staining method. Nocardia spp. were identified according to morphotinctorial characteristics.Susceptibility testing using the disc-diffusion method in agar (antibiogram) was performed using the following antibiotics:penicillin (10 IU), tetracycline (30 µg), amoxicillin (10 µg), gentamicin (10 µg), cephalexin (30 µg), erythromycin (15 µg),cephalothin (30 µg) and ampicillin (30 µg). However, the organism exhibited resistance to all drugs; as such, a new milksample was obtained at the same location the initial samples were collected. Samples (approximately 5 mL) were collectedaseptically and separately from all four teats in sterile bottles, during which the presence of granular material was noted.Bacterial culture was performed as...

Bovine mastitis caused by multidrug-resistant Nocardia farcinica

Background: Mastitis caused by Nocardia is characterized by pyogranulomatous inflammation related to inadequatehygiene conditions and is difficult to treat. Prompted by the absence of documentation of Nocardia farcinica associated tobovine mastitis in the Northeast region of Brazil, this is the first report to describe bovine mastitis caused by multidrugresistant N. farcinica.Case: Four milk samples (one from each teat) obtained from a 3-year-old Jersey cow raised on a property located in the metropolitan region of Recife, Pernambuco state, Brazil, were submitted to the Laboratory of Infectious-Contagious Diseases ofthe Veterinary Hospital at Universidade Federal Rural de Pernambuco [UFRPE]. At the laboratory, samples were cultured inbase agar enriched with 7% sheep blood (blood agar) in a microbiological incubator at 37°C under aerobic conditions for 72h. After only 48 h, however, pure bacterial colony growth was observed in all samples. Macroscopic analysis revealed smallcolonies, with an irregular shape, dry aspect, and greyish in color. Gram-positive rods forming filaments and/or ramificationswere observed using a Gram staining method. Nocardia spp. were identified according to morphotinctorial characteristics.Susceptibility testing using the disc-diffusion method in agar (antibiogram) was performed using the following antibiotics:penicillin (10 IU), tetracycline (30 µg), amoxicillin (10 µg), gentamicin (10 µg), cephalexin (30 µg), erythromycin (15 µg),cephalothin (30 µg) and ampicillin (30 µg). However, the organism exhibited resistance to all drugs; as such, a new milksample was obtained at the same location the initial samples were collected. Samples (approximately 5 mL) were collectedaseptically and separately from all four teats in sterile bottles, during which the presence of granular material was noted.Bacterial culture was performed as...(AU)

Peritonite piogranulomatosa por Nocardia concava em cão - relato de caso / Piogranulomatous peritonitis by Nocardia concava in dog - Case Report

Nocardiose é causada por bactérias do gênero Nocardia do subgrupo Actinomycetos, que são Gram-positivas aeróbicas, filamentosas e podem apresentar ramificações. O diagnóstico baseia-se na presença de lesão inflamatória, com o microrganismo morfologicamente compatível, associada ao isolamento e à identificação microbiológica e molecular. Este trabalho tem por objetivo relatar um caso de nocardiose em canino, que desenvolveu inflamação piogranulomatosa peritoneal seis meses após ovariossalpingo-histerectomia. O animal apresentava hipertermia, distensão abdominal, taquipneia, polidipsia, hiporexia, mucosas hipocoradas e fezes pastosas. Os achados laboratoriais evidenciaram anemia leve e leucocitose por neutrofilia com desvio à esquerda e hipoalbuminemia. Uma massa na região mesogástrica e efusão peritoneal foram evidenciadas por meio da ultrassonografia abdominal. O líquido foi classificado como exsudato piogranulomatoso, e o animal submetido à laparotomia exploratória para lavagem abdominal e remoção da massa. Após procedimentos terapêuticos, ocorreu piora clínica e óbito. Peritonite piogranulomatosa foi a principal alteração anatomopatológica a qual foi associada à Nocardia spp. Molecularmente, a espécie isolada se aproxima da N. concava, por meio da análise filogenética. Essa espécie já foi descrita como causa de infecção em humanos na Ásia, no entanto não há registros na literatura na espécie canina, sendo este o primeiro relato.(AU)

Nocardiosis is caused by an aerobic, gram-positive, ramificated and filamentous bacteria of the Nocardia genus, subgroup Actinomycetos. The diagnosis is based on the presence of the inflammatory lesions with the morphologically compatible microorganism associated with microbiological and molecular isolation and identification. The objective of this work is to report a case of nocardiosis in a canine that developed peritoneal pyogranulomatous inflammation six months after ovariosalpingohisterectomy. The animal had hyperthermia, abdominal distention, tachypnea, polydipsia, hyporexia, hypocorous mucosae and pasty feces. The laboratory findings revealed mild anemia and leukocytosis due to neutrophilia with left deviation and hypoalbuminemia. A mass in the mesogastric region and peritoneal effusion were evidenced by abdominal ultrasonography. The fluid was classified as pyogranulomatous exudate and the animal underwent exploratory laparotomy for abdominal lavage and mass removal. Despite the therapeutic procedures and clinical alterations the dog died. Piogranulomatous peritonitis was the main anatomopathological alteration which was associated with Nocardia spp. Molecularly, the isolated species approaches the N. concava species through phylogenetic analysis. This specie was described as a cause of infection in humans in Asia; however, there are no records in literature on the canine species, being this the first report.(AU)

Peritonite piogranulomatosa por Nocardia concava em cão - relato de caso / Piogranulomatous peritonitis by Nocardia concava in dog - Case Report

Nocardiose é causada por bactérias do gênero Nocardia do subgrupo Actinomycetos, que são Gram-positivas aeróbicas, filamentosas e podem apresentar ramificações. O diagnóstico baseia-se na presença de lesão inflamatória, com o microrganismo morfologicamente compatível, associada ao isolamento e à identificação microbiológica e molecular. Este trabalho tem por objetivo relatar um caso de nocardiose em canino, que desenvolveu inflamação piogranulomatosa peritoneal seis meses após ovariossalpingo-histerectomia. O animal apresentava hipertermia, distensão abdominal, taquipneia, polidipsia, hiporexia, mucosas hipocoradas e fezes pastosas. Os achados laboratoriais evidenciaram anemia leve e leucocitose por neutrofilia com desvio à esquerda e hipoalbuminemia. Uma massa na região mesogástrica e efusão peritoneal foram evidenciadas por meio da ultrassonografia abdominal. O líquido foi classificado como exsudato piogranulomatoso, e o animal submetido à laparotomia exploratória para lavagem abdominal e remoção da massa. Após procedimentos terapêuticos, ocorreu piora clínica e óbito. Peritonite piogranulomatosa foi a principal alteração anatomopatológica a qual foi associada à Nocardia spp. Molecularmente, a espécie isolada se aproxima da N. concava, por meio da análise filogenética. Essa espécie já foi descrita como causa de infecção em humanos na Ásia, no entanto não há registros na literatura na espécie canina, sendo este o primeiro relato.(AU)

Nocardiosis is caused by an aerobic, gram-positive, ramificated and filamentous bacteria of the Nocardia genus, subgroup Actinomycetos. The diagnosis is based on the presence of the inflammatory lesions with the morphologically compatible microorganism associated with microbiological and molecular isolation and identification. The objective of this work is to report a case of nocardiosis in a canine that developed peritoneal pyogranulomatous inflammation six months after ovariosalpingohisterectomy. The animal had hyperthermia, abdominal distention, tachypnea, polydipsia, hyporexia, hypocorous mucosae and pasty feces. The laboratory findings revealed mild anemia and leukocytosis due to neutrophilia with left deviation and hypoalbuminemia. A mass in the mesogastric region and peritoneal effusion were evidenced by abdominal ultrasonography. The fluid was classified as pyogranulomatous exudate and the animal underwent exploratory laparotomy for abdominal lavage and mass removal. Despite the therapeutic procedures and clinical alterations the dog died. Piogranulomatous peritonitis was the main anatomopathological alteration which was associated with Nocardia spp. Molecularly, the isolated species approaches the N. concava species through phylogenetic analysis. This specie was described as a cause of infection in humans in Asia; however, there are no records in literature on the canine species, being this the first report.(AU)

Características epidemiológicas, clínico-patológicas e morfotintoriais de quatorze casos de nocardiose em cães / Epidemiological, clinical, pathological and morphotinctorial features of fourteen cases of nocardiosis in dogs

Este artigo descreve os aspectos epidemiológicos, clínicos, patológicos e características morfotintoriais em quatorze casos de nocardiose em cães. Para isso foi realizado um estudo retrospectivo durante o período de janeiro de 2005 a dezembro de 2015 e selecionados os casos sugestivos de nocardiose. A identificação e caracterização do agente foi realizada através de técnicas histoquímicas especiais de Metenamina nitrato de prata de Grocott (GMS), Ziehl-Neelsen modificado, coloração de Gram do tipo Brown-Brenn modificado e Giemsa. Foram afetados predominantemente filhotes e em doze casos havia associação com o vírus da cinomose canina (VCC). Os sinais clínicos variaram de alterações respiratórias, neurológicas e cutâneas, relacionadas principalmente à infecção concomitante pelo VCC. Macroscopicamente haviam áreas multifocais a coalescentes, branco-amareladas, firmes, elevadas na superfície e que se aprofundavam ao corte, por vezes com material purulento, entremeadas por áreas avermelhadas irregulares afetando principalmente pulmões, linfonodos, fígado, rins e encéfalo. As lesões cutâneas foram observadas predominantemente nas regiões cervical e inguinal e variaram de supurativas a piogranulomatosas. No exame histopatológico a lesão era caracterizada principalmente por inflamação piogranulomatosa, porém em algumas áreas havia predomínio de inflamação necrossupurativa, e frequentemente eram observados macrófagos epitelioides, formando aglomerados de forma radiada, muitas vezes lembrando pseudorosetas. Nas técnicas histoquímicas foram observadas estruturas filamentosas, ramificadas, não septadas, medindo aproximadamente 1µm de espessura, impregnadas na coloração de prata, coradas em vermelho no Ziehl-Neelsen modificado, em azul na coloração de Gram do tipo Brown-Brenn modificado e fracamente rósea pálido no Giemsa. A nocardiose deve ser considerada em animais jovens que apresentam sinais respiratórios e neurológicos progressivos, bem como em lesões cutâneas com envolvimento do subcutâneo e linfonodos regionais. Deve ser investigado ainda um provável fator predisponente, como a infecção pelo vírus da cinomose canina e hemoparasitoses. O diagnóstico foi estabelecido através do exame histopatológico baseando-se na morfologia da bactéria e suas características histoquímicas, distinguindo principalmente de outros agentes bacterianos e fúngicos, constituindo uma importante ferramenta para o diagnóstico, quando não é possível a coleta do material para cultivo e isolamento do agente.(AU)

This paper describes the epidemiological, clinical, pathological and morphotinctorial characteristics in fourteen cases of nocardiosis in dogs. A retrospective study for the period of January 2005 to December 2015 was made and selected suggestive cases of nocardiosis. The identification and characterization of the agent was performed by special histochemical techniques Methenamine silver nitrate Grocott (GMS), modified Ziehl-Neelsen, Gram stain type modified Brown-Brenn and Giemsa. Were affected predominantly young and in twelve cases were associated with canine distemper virus (CDV). Clinical signs vary from respiratory, neurological and skin changes, mainly related to concomitant infection by CDV. Macroscopically had multifocal areas coalescing, yellowish-white, firm, elevated in surface and deepened the court, sometimes with purulent material, intermixed by irregular reddened areas affecting mainly lungs, lymph nodes, liver, kidneys, and brain. The cutaneous lesions were predominantly observed in cervical and inguinal and ranged from suppurative well as pyogranulomatous. In the histopathologic examination the injury was mainly characterized by inflammation pyogranulomatous, but in some areas there was a predominance of necrossupurativa inflammation, epithelioid macrophages and were frequently observed, forming clusters radiated form, often reminding rosettes. In the histochemical techniques were observed filamentous structures, branched, non-septate, measuring approximately 1µm thick, impregnated on silver staining, stained in red on the modified Ziehl-Neelsen, in blue on Gram stain type modified Brown-Brenn and weakly pale pink in Giemsa. The nocardiosis should be considered in young animals with progressive respiratory and neurological signs, as well as skin lesions involving the subcutaneous tissue and regional lymph nodes. It should be further investigated a possible predisposing factor, such as infection by canine distemper virus and hemoparasites. The diagnosis was established by histopathological examination based on the morphology of the bacteria and their histochemical characteristics, distinguishing mainly from other bacterial and fungal agents and is an important tool for the diagnosis, when the collection of material for cultivation and isolation of the agent is not possible.(AU)

Características epidemiológicas, clínico-patológicas e morfotintoriais de quatorze casos de nocardiose em cães / Epidemiological, clinical, pathological and morphotinctorial features of fourteen cases of nocardiosis in dogs

Este artigo descreve os aspectos epidemiológicos, clínicos, patológicos e características morfotintoriais em quatorze casos de nocardiose em cães. Para isso foi realizado um estudo retrospectivo durante o período de janeiro de 2005 a dezembro de 2015 e selecionados os casos sugestivos de nocardiose. A identificação e caracterização do agente foi realizada através de técnicas histoquímicas especiais de Metenamina nitrato de prata de Grocott (GMS), Ziehl-Neelsen modificado, coloração de Gram do tipo Brown-Brenn modificado e Giemsa. Foram afetados predominantemente filhotes e em doze casos havia associação com o vírus da cinomose canina (VCC). Os sinais clínicos variaram de alterações respiratórias, neurológicas e cutâneas, relacionadas principalmente à infecção concomitante pelo VCC. Macroscopicamente haviam áreas multifocais a coalescentes, branco-amareladas, firmes, elevadas na superfície e que se aprofundavam ao corte, por vezes com material purulento, entremeadas por áreas avermelhadas irregulares afetando principalmente pulmões, linfonodos, fígado, rins e encéfalo. As lesões cutâneas foram observadas predominantemente nas regiões cervical e inguinal e variaram de supurativas a piogranulomatosas. No exame histopatológico a lesão era caracterizada principalmente por inflamação piogranulomatosa, porém em algumas áreas havia predomínio de inflamação necrossupurativa, e frequentemente eram observados macrófagos epitelioides, formando aglomerados de forma radiada, muitas vezes lembrando pseudorosetas. Nas técnicas histoquímicas foram observadas estruturas filamentosas, ramificadas, não septadas, medindo aproximadamente 1µm de espessura, impregnadas na coloração de prata, coradas em vermelho no Ziehl-Neelsen modificado, em azul na coloração de Gram do tipo Brown-Brenn modificado e fracamente rósea pálido no Giemsa. A nocardiose deve ser considerada em animais jovens que apresentam sinais respiratórios e neurológicos progressivos, bem como em...(AU)

This paper describes the epidemiological, clinical, pathological and morphotinctorial characteristics in fourteen cases of nocardiosis in dogs. A retrospective study for the period of January 2005 to December 2015 was made and selected suggestive cases of nocardiosis. The identification and characterization of the agent was performed by special histochemical techniques Methenamine silver nitrate Grocott (GMS), modified Ziehl-Neelsen, Gram stain type modified Brown-Brenn and Giemsa. Were affected predominantly young and in twelve cases were associated with canine distemper virus (CDV). Clinical signs vary from respiratory, neurological and skin changes, mainly related to concomitant infection by CDV. Macroscopically had multifocal areas coalescing, yellowish-white, firm, elevated in surface and deepened the court, sometimes with purulent material, intermixed by irregular reddened areas affecting mainly lungs, lymph nodes, liver, kidneys, and brain. The cutaneous lesions were predominantly observed in cervical and inguinal and ranged from suppurative well as pyogranulomatous. In the histopathologic examination the injury was mainly characterized by inflammation pyogranulomatous, but in some areas there was a predominance of necrossupurativa inflammation, epithelioid macrophages and were frequently observed, forming clusters radiated form, often reminding rosettes. In the histochemical techniques were observed filamentous structures, branched, non-septate, measuring approximately 1µm thick, impregnated on silver staining, stained in red on the modified Ziehl-Neelsen, in blue on Gram stain type modified Brown-Brenn and weakly pale pink in Giemsa. The nocardiosis should be considered in young animals with progressive respiratory and neurological signs, as well as skin lesions involving the subcutaneous tissue and regional lymph nodes. It should be further investigated a...(AU)

Características epidemiológicas, clínico-patológicas e morfotintoriais de quatorze casos de nocardiose em cães

ABSTRACT: This paper describes the epidemiological, clinical, pathological and morphotinctorial characteristics in fourteen cases of nocardiosis in dogs. A retrospective study for the period of January 2005 to December 2015 was made and selected suggestive cases of nocardiosis. The identification and characterization of the agent was performed by special histochemical techniques Methenamine silver nitrate Grocott (GMS), modified Ziehl-Neelsen, Gram stain type modified Brown-Brenn and Giemsa. Were affected predominantly young and in twelve cases were associated with canine distemper virus (CDV). Clinical signs vary from respiratory, neurological and skin changes, mainly related to concomitant infection by CDV. Macroscopically had multifocal areas coalescing, yellowish-white, firm, elevated in surface and deepened the court, sometimes with purulent material, intermixed by irregular reddened areas affecting mainly lungs, lymph nodes, liver, kidneys, and brain. The cutaneous lesions were predominantly observed in cervical and inguinal and ranged from suppurative well as pyogranulomatous. In the histopathologic examination the injury was mainly characterized by inflammation pyogranulomatous, but in some areas there was a predominance of necrossupurativa inflammation, epithelioid macrophages and were frequently observed, forming clusters radiated form, often reminding rosettes. In the histochemical techniques were observed filamentous structures, branched, non-septate, measuring approximately 1µm thick, impregnated on silver staining, stained in red on the modified Ziehl-Neelsen, in blue on Gram stain type modified Brown-Brenn and weakly pale pink in Giemsa. The nocardiosis should be considered in young animals with progressive respiratory and neurological signs, as well as skin lesions involving the subcutaneous tissue and regional lymph nodes. It should be further investigated a possible predisposing factor, such as infection by canine distemper virus and hemoparasites. The diagnosis was established by histopathological examination based on the morphology of the bacteria and their histochemical characteristics, distinguishing mainly from other bacterial and fungal agents and is an important tool for the diagnosis, when the collection of material for cultivation and isolation of the agent is not possible.

RESUMO: Este artigo descreve os aspectos epidemiológicos, clínicos, patológicos e características morfotintoriais em quatorze casos de nocardiose em cães. Para isso foi realizado um estudo retrospectivo durante o período de janeiro de 2005 a dezembro de 2015 e selecionados os casos sugestivos de nocardiose. A identificação e caracterização do agente foi realizada através de técnicas histoquímicas especiais de Metenamina nitrato de prata de Grocott (GMS), Ziehl-Neelsen modificado, coloração de Gram do tipo Brown-Brenn modificado e Giemsa. Foram afetados predominantemente filhotes e em doze casos havia associação com o vírus da cinomose canina (VCC). Os sinais clínicos variaram de alterações respiratórias, neurológicas e cutâneas, relacionadas principalmente à infecção concomitante pelo VCC. Macroscopicamente haviam áreas multifocais a coalescentes, branco-amareladas, firmes, elevadas na superfície e que se aprofundavam ao corte, por vezes com material purulento, entremeadas por áreas avermelhadas irregulares afetando principalmente pulmões, linfonodos, fígado, rins e encéfalo. As lesões cutâneas foram observadas predominantemente nas regiões cervical e inguinal e variaram de supurativas a piogranulomatosas. No exame histopatológico a lesão era caracterizada principalmente por inflamação piogranulomatosa, porém em algumas áreas havia predomínio de inflamação necrossupurativa, e frequentemente eram observados macrófagos epitelioides, formando aglomerados de forma radiada, muitas vezes lembrando pseudorosetas. Nas técnicas histoquímicas foram observadas estruturas filamentosas, ramificadas, não septadas, medindo aproximadamente 1µm de espessura, impregnadas na coloração de prata, coradas em vermelho no Ziehl-Neelsen modificado, em azul na coloração de Gram do tipo Brown-Brenn modificado e fracamente rósea pálido no Giemsa. A nocardiose deve ser considerada em animais jovens que apresentam sinais respiratórios e neurológicos progressivos, bem como em lesões cutâneas com envolvimento do subcutâneo e linfonodos regionais. Deve ser investigado ainda um provável fator predisponente, como a infecção pelo vírus da cinomose canina e hemoparasitoses. O diagnóstico foi estabelecido através do exame histopatológico baseando-se na morfologia da bactéria e suas características histoquímicas, distinguindo principalmente de outros agentes bacterianos e fúngicos, constituindo uma importante ferramenta para o diagnóstico, quando não é possível a coleta do material para cultivo e isolamento do agente.

Pyogranulomatous dermatitis and panniculitis due to Nocardia nova in a cat / Dermatite e paniculite piogranulomatosas causadas por Nocardia nova em um gato

Este relato descreve os achados clínicos, patológicos e microbiológicos de uma infecção incomum por Nocardia nova em um gato. Um gato macho, sem raça definida, de 3 anos de idade, apresentou ferida cutânea exsudativa e ulcerada. Amostras da lesão foram coletadas para histopatologia e bacteriologia. Histologicamente, a lesão consistiu de dermatite e paniculite piogranulomatosas associadas a colônias grandes e irregulares de bactérias filamentosas e ramificadas. O cultivo bacteriológico revelou bacilos filamentosos, gram-positivos, parcialmente ácido resistentes, visualizados pelas colorações de Gram e Kinyoun, respectivamente. A identificação da Nocardia novafoi confirmada pelo sequenciamento 16S rDNA e análise filogenética. Este é o primeiro caso de paniculite e dermatite piogranulomatosas em um gato causado por Nocardia nova registrado no Brasil.(AU)

This report describes the clinical, pathological and microbiological findings of an uncommon infection in a cat by Nocardia nova. A 3-year-old male domestic short hair cat with an ulcerated and exudative cutaneous wound was presented for clinical examination. Samples were collected for histopathology and bacteriology diagnosis. Microscopically, the lesion was diagnosed as pyogranulomatous dermatitis and panniculitis with large and irregular colonies of branching filamentous bacterium. Skin bacteriological culture showed gram-positive rods and partially acid-fast branching filaments by gram and kinyoun staining, respectively. The identity of Nocardia nova was confirmed by 16S rDNA sequencing and phylogenetic analysis. This is the first case of pyogranulomatous dermatitis and panniculitis in a cat caused byNocardia nova reported in Brazil.(AU)

Esporotricose felina: distribuição das lesões e caracterização anatomopatológica utilizando diversos métodos de diagnóstico

A esporotricose é uma infecção fúngica crônica profunda da pele causada pelo gênero Sporothrix spp. Pode se apresentar na forma cutânea, linfo-cutânea e extracutânea, embora esta última ainda sem muitos estudos e esclarecimentos. O objetivo desse trabalho foi caracterizar por histopatologia e imuno-histoquímica, as lesões cutâneas e extracutâneas da esporotricose e comparar os diferentes métodos diagnósticos em gatos da região metropolitana de Belo Horizonte. No período de março de 2018 a março de 2019, foram avaliados 110 gatos resgatados pelo Centro de Controle de Zoonoses de Belo Horizonte, com suspeita clínica de esporotricose felina, confirmada ou não por cultivo e isolamento. Os gatos com impossibilidade de serem tratados foram eutanasiados. Após a eutanásia, os animais foram avaliados por exames macroscópico, citopatológico, histopatológico e imuno-histoquímico. Testes moleculares para a confirmação da espécie de Sporothrix também foram realizados. Dos 110 animais, 74 foram positivos para Sporothrix spp. por coloração de rotina, 30 por imuno-histoquímica e 2 somente por cultura fúngica e PCR. A citopatologia e a histopatologia foram eficientes no diagnóstico da doença, embora em gatos com lesões contendo carga fúngica baixa, a imuno-histoquímica foi necessária para a confirmação do agente. Os achados histopatológicos mais comuns na pele foram: dermatite piogranulomatosa, dermatite histiocitária e plasmocitária e dermatite granulomatosa, com intensidades variadas na quantidade de leveduras nas lesões. Os achados extracutâneos foram rinite/rinosinusite histiocitária e plasmocitária ou piogranulomatosa, linfadenite histiocitária sinusoidal, linfadenite granulomatosa, pneumonia histioplasmocitária e neutrofílica, conjuntivite histiolinfoplasmocitária, glossite granulomatosa, periorquite granulomatosa e necrosante e meningite histiocitária, todos com presença de leveduras em diferentes intensidades. Em quatro dos 110 gatos com lesões cutâneas ulcerativas, foram diagnosticados três casos com carcinoma de células escamosas e um caso de nocardiose cutânea, sendo considerados importantes para o diagnóstico diferencial

Sporotrichosis is a chronic deep fungal infection of the skin caused by the genus Sporothrix spp. The disease has cutaneous, lympho-cutaneous and extracutaneous forms, although the last one has not been studied and clarified yet. The aim of this study was to characterize by histopathology and immunohistochemistry exams, the cutaneous and extracutaneous lesions of sporotrichosis in cats from metropolitan region of Belo Horizonte. In the period from March 2018 to March 2019, 110 cats rescued by the Zoonoses Control Center of Belo Horizonte, were evaluated with clinical suspicion of feline sporotrichosis, confirmed or not by culture and isolation. Cats unable to be treated were euthanized. After euthanasia, the animals were evaluated by macroscopic, cytopathological, histopathological and immunohistochemical exams. Molecular tests to confirm the Sporothrix species were also performed. Out of 110 animals, 74 were positive for Sporothrix spp. on routine hematoxylin and eosin staining, 30 by immunohistochemistry and two only by fungal culture and PCR. Cytopathology and histopathology exams were efficient in the diagnosis of the disease, although in cats with lesions containing low fungal load, immunohistochemistry was needed to confirm the agent. The most common histopathological findings on the skin were pyogranulomatous dermatitis, histiocytic and plasmacytic dermatitis and granulomatous dermatitis, with different amount of yeasts in the lesions. The extracutaneous findings were histiocytic and plasmacytic or pyogranulomatous rhinitis/rhinosinusitis, sinusoidal histiocytic lymphadenitis, granulomatous lymphadenitis, histioplasmacytic and neutrophilic pneumonia, histiolymphoplasmacytic conjunctivitis, granulomatous glossitis, granulomatous and necrotic periorchitis and histiocytic meningitis, all with many different numbers of yeasts. In four of the 110 cats with ulcerative skin lesions, other diseases were diagnosed; three cases of squamous cell carcinoma and one case of cutaneous nocardiosis, being considered important for the differential diagnosis.

Nocardiose cutânea em felino: relato de caso / Feline cutaneous nocardiosis: case report

A nocardiose cutânea é uma condição supurativa a piogranulomatosa, que ocorre após penetração do agente em ferimentos napele que foram expostas a água ou ao solo contaminado. A doença se manifesta com formação de abscessos localizados, comfístulas e úlceras necróticas que drenam uma secreção serossanguinolenta, atingindo membros ou abdômen. É rara em gatos, enestes, a maior parte se deve a infecção por Nocardia asteroides. Este relato objetivou descrever o diagnóstico clínico e laboratorialde um felino doméstico com diagnóstico microbiológico de Nocardia asteroides, descrevendo o tratamento preconizado combase no antibiograma. Uma gata castrada, sem raça definida (SRD), com cinco anos de idade, desenvolveu lesão cutânea emforma de tratos fistulosos drenando secreção piossanguinolenta sob a região frontal da face, membros torácicos e abscesso emmembro pélvico, além de inapetência, emagrecimento e apatia. Havia sido tratada anteriormente de forma empírica para infecçãobacteriana com enrofloxacina, seguida de cefalexina após identificação bacteriana de Staphylococcus spp. Realizou-se coleta doexsudato para fins de cultura e antibiograma. O diagnóstico foi de nocardiose e o agente identificado como Nocardia asteroidese os micro-organismos foram sensíveis apenas a amicacina, neomicina e canamicina. Foi instituído tratamento com amicacina(10 mg/kg, a cada 12 horas), passando após sete dias a ser administrado a cada 24 horas devido a cilindrúria constatada atravésde urinálise. A remissão dos sinais ocorreu com 20 dias de uso contínuo do antibiótico, o qual foi mantido por mais três semanassem outras complicações com resolução da cilindrúria.(AU)

The cutaneous nocardiosis is a suppurative to pyogranulomatous condition that occurs after penetration of the agent on woundsin the skin that were exposed to contaminated water or soil. The disease is manifested with formation of localized abscess, withnecrotic fistula and ulcers that draining a serosanguineous secretion, reaching limbs or abdomen. It is rare in cats, and in these, themost are due to infection by Nocardia asteroides. This report aimed to describe the clinical and laboratory diagnosis of a domesticfeline with microbiological diagnosis of Nocardia asteroides, describing the recommended treatment based on antibiogram. A spayedfemale cat, mongrel, with five years of age, developed cutaneous lesions in the form of fistulous tracts draining piosanguinolentasecretion on the frontal region of the face, forelimb and abscess in hind limb, and loss of appetite, weight loss and apathy. It hadpreviously been treated empirically for bacterial infections with enrofloxacin, followed by cephalexin after bacterial identification ofStaphylococcus spp. Held collection of exudate for culture and antibiogram. It was diagnosed nocardiosis and the identified agentas Nocardia asteroides and microrganisms were sensitive only to amikacin, kanamycin and neomycin. Treatment with amikacin (10mg / kg every 12 hours) was introduced, and seven days after being administered once every 24 hours due to cilindruria detectedby urinalysis. The remission of the signs occurred 20 days of continuous use of the antibiotics, which was maintained for threeweeks without further complications with resolution of the cilindruria.(AU)

ACTINOMICETOS DIAGNOSTICADOS EM FELINOS NO SERTÃO DA PARAÍBA

Vários actinomicetos são responsáveis por desenvolverem doenças em animais domésticos e no homem, causando diversas manifestações clínicas, que podem evoluir para a morte dos animais afetados. Geralmente as características das lesões causadas por esses microrganismos são semelhantes, cuja função da resposta tecidual do hospedeiro visa circundar o microrganismo em contingente celular delimitado por cápsula fibrosa, com vistas a impedir sua dispersão via sistêmica ou por ação direta do agente na região circunvizinha. No exame histopatológico, é possível identificar uma arquitetura celular típica, formando granulomas e/ou piogranulomas. Esta dissertação apresentada ao Programa de Pós-Graduação em Medicina Veterinária da Universidade Federal de Campina Grande é composta por dois capítulos constituídos por artigos científicos. O primeiro capítulo submetido à revista Pesquisa Veterinária Brasileira e o segundo submetido ao Arquivo Brasileiro de Medicina Veterinária e Zootecnia. O primeiro artigo dessa dissertação descreve as características clínicas, patológicas, imuno-histoquímicas e moleculares de micobactérias em felinos diagnosticados no sertão da Paraíba. O segundo descreve quatro casos de nocardiose em felinos domésticos, abordando as principais características clínicas, anatomopatológicas e morfotintoriais da Nocardia spp. São doenças que ocorrem esporadicamente em gatos e que nos últimos anos vem aumentando o diagnóstico no Laboratório de Patologia Animal da Universidade Federal de Campina Grande. É importante ressaltar o diagnóstico de tuberculose felina por Mycobacterium bovis para a saúde pública, por possuir potencial zoonótico e muitos dos animais infectados apresentarem sinais clínicos inespecíficos. E que as infecções por micobactérias e por Nocardia spp. devem ser incluídas no diagnóstico diferencial de felinos com emagrecimento progressivo, linfadenopatia, dispneia, piotórax, peritonite e sinais neurológicos.

Several actinomycetes are responsible for developing diseases in domestic animals and humans. Causing various clinical manifestations, which may evolve to the death of affected animals. Generally the characteristics of the lesions caused by these microorganisms are similar, whose function of the host tissue response is to surround the microorganism in a cellular contingent delimited by a fibrous capsule, in order to prevent its dispersion via systemic or direct action of the agent in the surrounding region. In histopathological examination, it´s possible to identify a typical cellular architecture, forming granulomas and/or piogranulomas. This dissertation presented to the Graduate Program in Veterinary Medicine of the Federal University of Campina Grande is composed by two chapters constituted by cientific articles. The first chapter was submitted to the Brazilian Veterinary Research Magazine and the second one submitted to the Brazilian Archive of Veterinary Medicine and Animal Science. The first article of this dissertation describes the clinical, pathological, Immunohistochemistry and molecular characteristics of mycobacteria in felines diagnosed in the Backland od Paraiba, Brazil. The second article describes four cases of nocardiosis in domestic felines, approaching the main clinical, pathological and morphotintorial features of Nocardia spp. These diseases are sporadically diagnosed in cats and in recent years have been increasing their occurrence in the Animal Pathology Laboratory of the Federal University of Campina Grande. Its importante to emphasize the diagnosis of feline tuberculosis caused by Mycobacterium bovis for local public health because it has zoonotic potential and many of the infected animals show nonspecific clinical signs. And that infections by mycobacteria and by Nocardia spp. should be included in the differential diagnosis of felines with progressive weight loss, lymphadenopathy, dyspnea, piothorax, peritonitis and neurological signs.

DOENÇAS DO SISTEMA NERVOSO CENTRAL E INFECÇÕES OPORTUNISTAS DIAGNOSTICADAS EM CÃES NO LABORATÓRIO DE PATOLOGIA ANIMAL DO HOSPITAL VETERINÁRIO/UFCG, PATOS, PARAÍBA

Descrevem-se nesta tese três artigos científicos relacionados a doenças de cães diagnosticadas no Laboratório de Patologia Animal, do Hospital Veterinário, da Universidade Federal de Campina Grande (LPA/HV/UFCG), Campus de Patos, Paraíba. No primeiro capítulo descrevem-se as doenças do sistema nervoso central diagnosticadas em cães durante o período de janeiro de 2003 a dezembro de 2014. Verificaram-se nesse estudo 354 casos (29,37%) com alterações neurológicas, desses 296 (83,61%) com o diagnóstico definitivo e 58 casos (16,39%) inconclusivos. As principais doenças verificadas foram as infecciosas [59,60% (211/354)], seguidas dos distúrbios causados por agentes físicos [9,89% (35/354)] e neoplasias primárias e secundárias [5,93% (21/354)]. Também foram observadas alterações neurológicas secundárias a distúrbios metabólicos em decorrência de insuficiência hepática e renal [2,54% (9/354)], além de hidrocefalia congênita [1,41% (5/354)]. Verificaram-se ainda manifestações neurológicas associadas a lesões vasculares, degenerativas e inflamatórias não infecciosas, dos quais muitos não foram possíveis estabelecer uma causa específica que representaram 4,24% (15/354), sendo infartos isquêmicos e hemorrágicos (6/15), vasculite e necrose fibrinoide (5/15), doença do disco intervertebral (2/15), meningoencefalite granulomatosa (1/15) e granuloma de colesterol (1/15). No segundo capítulo descrevem-se os aspectos epidemiológicos, clínicos, patológicos e características morfotintoriais em quatorze casos de nocardiose em cães diagnosticados durante o período de janeiro de 2005 a dezembro de 2015. Foram afetados predominantemente filhotes e em doze casos havia associação com o vírus da cinomose canina (VCC). Os sinais clínicos variaram de alterações respiratórias, neurológicas e cutâneas, relacionadas principalmente à infecção concomitante pelo VCC. Macroscopicamente haviam áreas multifocais a coalescentes, branco-amareladas, firmes, elevadas na superfície e que se aprofundavam ao corte, por vezes com material purulento, entremeadas por áreas avermelhadas irregulares afetando principalmente pulmões, linfonodos, fígado, rins e encéfalo. As lesões cutâneas foram observadas predominantemente nas regiões cervical e inguinal e variaram de supurativas a piogranulomatosas. No exame histopatológico a lesão era caracterizada principalmente por inflamação piogranulomatosa, porém em algumas áreas havia predomínio de inflamação necrossupurativa, e frequentemente eram observados macrófagos epitelioides, formando aglomerados de forma radiada, muitas vezes lembrando pseudorosetas. Nas técnicas histoquímicas foram observadas estruturas filamentosas, ramificadas, não septadas, medindo aproximadamente 1 m de espessura, impregnadas na coloração de prata, coradas em vermelho no Ziehl-Neelsen modificado, em azul na coloração de Gram do tipo Brown-Brenn modificado e fracamente rósea pálido no Giemsa, compatíveis com infecção bacteriana pelo gênero Nocardia. No terceiro capítulo descreve-se um caso de colite necrohemorrágica em um canino causada por Entamoeba histolytica associada à infecção pelo vírus da cinomose, caracterizando os aspectos epidemiológicos e clínico-patológicos. A doença ocorreu em uma cadela errante, sem raça definida, adulta que apresentou anorexia, ataxia, estado geral ruim com desidratação de 12%, secreção ocular e nasal bilateral purulenta. Foi estabelecido diagnóstico clínico de cinomose e instituído o tratamento. O quadro evoluiu para andar em círculos, andar a esmo, sonolência e cegueira. Após três dias do início dos sinais neurológicos, também apresentou diarreia com fezes amolecidas e sanguinolentas. Sem melhora do quadro, optou-se pela eutanásia. Na necropsia observou-se animal magro com edema subcutâneo. Pulmões com áreas de consolidação nas porções crânio-ventrais, amareladas que ao corte apresentava secreção purulenta. No intestino grosso observou-se distensão segmentar na porção final do cólon descendente de aproximadamente 15 cm de extensão e a serosa avermelhada e irregular, além de discreto espessamento da parede e mucosa edemaciada com coágulos de sangue, fibrina e múltiplos focos de ulcerações. Microscopicamente no intestino havia área focalmente extensa de necrose, hemorragia, hiperplasia e dilatação das vilosidades na mucosa. Nessas áreas eram observadas estruturas arredondadas de citoplasma abundante eosinofílico, levemente granular ou vacuolizado, com núcleo central ou ligeiramente excêntrico e nucléolo em forma de alvo, que mediam aproximadamente 15 m de diâmetro, compatíveis com trofozoítos de amebas. Havia também encefalite desmielinizante associado à malacia e corpúsculos de inclusões eosinofílicos intranucleares e/ou intracitoplasmáticos em células ependimárias, gemistócitos e astrócitos, característicos de infecção pelo vírus da cinomose. Na imuno-histoquímica com anticorpo policlonal anti-E. histolytica na diluição de 1:1000 os trofozoítos foram imunomarcados, confirmando a suspeita de amebíase. Dentre as doenças diagnosticadas em cães no LPA/HV/UFCG, verificou-se que as infecciosas ocorrem mais comumente representando uma importante causa de morte na região. Destas, a cinomose foi a mais frequente e constituiu uma importante causa de imunossupressão predispondo os animais a infecções secundárias, resultando em complicações sistêmicas graves.

We describe in this thesis three scientific papers related to dog diseases diagnosed in the Laboratory of Animal Pathology, Veterinary Hospital of the Federal University of Campina Grande (LPA/HV/UFCG), Campus of Patos, Paraíba. The first chapter describes the central nervous system diseases diagnosed in dogs during the period from January 2003 to December 2014. Have verified in this study 354 cases (29.37%) with neurological disorders, these 296 (83.61%) with a definitive diagnosis and 58 cases (16.39%) inconclusive. The main infectious diseases were checked [59.60% (211/354)], followed by disturbances caused by physical agents [9.89% (35/354)] and primary and secondary tumors [5.93% (21/354)]. It was also observed secondary neurological disorders to metabolic disorders due to liver and kidney failure [2.54% (9/354)], and congenital hydrocephalus [1.41% (5/354)]. There were neurological manifestations associated with vascular, degenerative and non-infectious inflammatory injuries, many of which were not possible to establish a specific cause which accounted for 4.24% (15/354), which were ischemic and hemorrhagic infarcts (6/15), vasculitis and fibrinoid necrosis (5/15), intervertebral disc disease (2/15), granulomatous meningoencephalitis (1/15) and cholesterol granuloma (1/15). In the second chapter describes the epidemiological, clinical, pathological and morphotinctorial characteristics in fourteen cases of nocardiosis in dogs diagnosed during the period from January 2005 to December 2015. Were affected predominantly young and in twelve cases were associated with virus distemper (VCC). Clinical signs ranged from respiratory, neurological and skin changes, mainly related to concomitant infection VCC. Macroscopically had multifocal areas coalescing, yellowish-white, firm, elevated in surface and deepened the court, sometimes with purulent material, interspersed with irregular reddened areas mainly affecting the lungs, lymph nodes, liver, kidneys and brain. The cutaneous lesions were predominantly observed in cervical and inguinal and ranged from suppurative to pyogranulomatous. In the histopathology injury it was mainly characterized by inflammation pyogranulomatous, but in some areas there was a predominance of necrossupurativa inflammation, epithelioid macrophages and were frequently observed, forming clusters of radiated form, often reminding pseudorosetas. In the histochemical techniques were observed filamentous structures, branched, non-septate, measuring approximately 1 m thick, impregnated on silver staining, stained in red on the modified Ziehl-Neelsen, in blue on Gram stain type modified Brown-Brenn and weakly pale pink in Giemsa, compatible with bacterial infection by genus Nocardia. In the third chapter describes a case of necro-hemorrhagic colitis in a canine caused by Entamoeba histolytica associated with infection by canine distemper virus, characterizing the epidemiological, clinicopathological and immunohistochemical. The disease occurred in an errant bitch, mongrel adult who presented anorexia, ataxia, poor general condition with 12% dehydration, eye discharge and purulent nasal bilateral. It was established clinical diagnosis of distemper and treatment instituted. Patient evolved to walk in circles, walking aimlessly, drowsiness and blindness. After three days of onset of neurological signs, also presented diarrhea with loose and bloody stools. No improvement, we opted for euthanasia. At necropsy there was lean animal with subcutaneous edema. Lungs with areas of consolidation in the cranio-ventral portions, yellow that the court had purulent secretion. In the large intestine was observed segmental distention in the final portion of the descending colon of approximately 15 cm in length and irregular reddish serous and mild thickening of the mucosa edema with blood clots, fibrin and multiple ulcerations foci. Microscopically intestine was focally extensive area of necrosis, hemorrhage and hyperplasia of villi on mucosa swelling. These areas were observed rounded structures of eosinophilic abundant cytoplasm, slightly granular or vacuolated, with core or slightly eccentric and nucleolus-shaped target, which measured approximately 15 m in diameter, consistent with trophozoites of amoebae. There was also demyelinating encephalitis associated with malacic and corpuscles intranuclear eosinophilic inclusions and / or intracytoplasmic in ependymal cells, astrocytes and gemistocytes characteristic of infection with canine distemper virus. In immunohistochemistry with polyclonal anti-E. histolytica in the dilution of 1:1000 trophozoites were immunomarked, confirming the suspected amebiasis. Among the diseases diagnosed in dogs in semiarid Paraiba, it was found that the most commonly occurring infectious representing a major cause of death in the region. Of these, distemper was the most frequent and constituted an important cause of immunosuppression predisposing animals to secondary infections resulting in severe systemic complications.

Caracterização fenotípica, genotípica e termorressistência à fervura em linhagens de Nocardia spp isoladas de animais domésticos e humanos

Nocardia é um microorganismo ubíquo, relacionado a processos piogranulomatosos supurativos de difícil resolução terapêutica em humanos e animais. Foi investigada caracterização fenotípica, genotípica, perfil de sensibilidade aos antimicrobianos, e termorresistência à fervura em 95 linhagens de Nocardia spp. provenientes de animais domésticos e humanos. Em animais de produção a afecção mais comum foi mastite bovina identificada em 71 linhagens (74,73%). Em animais de companhia obteve-se mais isolamento nos cães com 12 linhagens (12,63%), principalmente de tecido cutâneo. Foram estudados sete isolados de Nocardia spp. obtidos de humanos, com quadros cutâneos e neurológicos. A caracterização molecular e análise filogenética realizada por PCR da fração 16S rRNA identificaram as seguintes espécies: Nocardia nova (68,42%), Nocardia farcinica (13,68%), Nocardia cyriacigeorgica (4,58%), Nocardia puris (3,67%), Nocardia veterana (3,67%), Nocardia africana (2,10%), Nocardia asiatica (0,91%) e Nocardia arthritidis (0,91%). A ocorrência de multirresistência a três ou mais e a cinco ou mais antimicrobianos ocorreu em, respectivamente, 42,88% e 14,28%. A comparação entre perfil de sensibilidade por disco-difusão e concentração inibitória mínima mostrou correlação fraca, exceto para amicacina. Na avaliação da termorresistência à fervura foi observado reisolamento de 84,21% das linhagens após atingir 100ºC no leite, 50,52% após um minuto de fervura a 100ºC e 2,10% após cinco minutos. Depreendeu-se no estudo alta similaridade entre linhagens de Nocardia sp. isoladas de animais domésticos e humanos, elevada multirresistência aos antimicrobianos utilizados na terapia da nocardiose em animais e humanos, e a termorresistência à fervura do leite, ressaltando riscos de consumo do leite de animais com nocardiose mamária

Nocardia is an ubiquitous microorganism, environmental, related to supurative pyogranulomatous of difficult treatment in humans and domestic animals. The phenotypic and genotypic characterization, antimicrobial susceptibility profile and thermorresistance to boiling of 95 strains of Nocardia spp., from both domestic animals and humans, were investigated. In production animals the most common affection was the bovine mastitis, identified in 71 strains (74,73%). Among the small animal, most of the isolates were from dogs, a total of 12 strains (12,63%), especially from cutaneous manifestations. Seven isolates were obtained from humans, with emphasis for cases with cutaneous and neurological signs. The molecular characterization and the phylogenetic analysis made through PCR of the 16S rRNA resulted in the identification: Nocardia nova (68,42%), Nocardia farcinica (13,68%), Nocardia cyriacigeorgica (4,58%), Nocardia puris (3,67%), Nocardia veterana (3,67%), Nocardia africana (2,10%), Nocardia asiatica (0,91%) and Nocardia arthritidis (0,91%). The occurrence of multi-resistance to three or more and five or more antimicrobials was observed in 42,88% and 14,28%of the studied strains, respectively. The comparison between the susceptibility profile through disc diffusion and the minimum inhibitory concentration showed a weak correlation, except for amikacin. In the evaluation of thermorresistance to boiling 84,21% of the strains were re-isolated after reaching 100°C in milk, 50,52% after boiling for one minute at 100ºC and 2,10% after boiling for 5 minutes. This study showed high similarity between Nocardia sp. strains isolated from domestic animals and humans, increased multi-resistance to some of the most used antimicrobials in the therapy of nocardiosis in animals and humans, thermorresistance to the boiling conditions of milk, emphasizing the risk of consuming products from animals with mammary nocardiosis