Effect of Ammonia Gas in Poultry Litter Contaminated with Salmonella Heidelberg

Salmonella Heidelberg is an emerging pathogen in Brazilian poultry production. The traditional methods (quicklime, windrowing and tarpaulin-on-surface) used for disinfecting reused poultry litter between flocks does not guarantee its elimination, thus allowing the transmission of this agent from one flock to another. The new tarpaulinon-surface method with controlled injection of ammonia gas has proven to be effective in its control, however, it is still unknown what dose of ammonia gas is needed to eliminate Salmonella Heidelberg in reused poultry litter. The objective of this study was to evaluate the effect of ammonia gas at different concentrations in sterile poultry litter artificially contaminated with Salmonella Heidelberg. Then, ammonia gas was injected in concentrations of 0.25%, 0.5%, and 1%, and 48 hours later, a sample was collected from each repetition in an entirely randomized design, and bacterial isolation was performed. All treatments, including positive and negative controls, were tested in quadruplicate and the parameters temperature, humidity, pH and water activity were evaluated. In the 0.5% and 1% treated samples the pathogen was not isolated, while in the 0.25% concentration one of the four samples tested was positive. The study reveals that ammonia gas is efficient in killing Salmonella Heidelberg in poultry litter at concentrations of 0.5 % or more within a 48-hour period and that the litter treated with ammonia gas increases its pH and water activity.(AU)

Physical and physiological qualities and productivity of corn seeds fertilized with poultry waste / Avaliação das qualidades física e fisiológica e da produtividade de sementes de milho adubado com resíduo avícola

ABSTRACT: This study evaluated the physical and physiological qualities and productivity of corn seeds, a variety UFVM 100 Native, produced in plots fertilized in top dressing with different levels of poultry waste. The experiment was organized in a randomized block design, with six treatments and four repetitions, totaling 24 plots. The treatments consisted of the application of 0.0, 1.5, 3.0, 4.5, 6.0, and 7.5 t/ha of tanned poultry waste. The seeds were subjected to the following evaluations: uniformity test, thousand seed weight, germination, vigor (first germination count, accelerated aging, soilless cold test, percentage, and emergence rate in sand), and productivity. Linear regression models were used to evaluate the effects of different dosages of poultry waste on the variables considered. The quality of seeds was not influenced by the effects of different levels of fertilization. Among the dosages used, 7.5 t/ha is recommended because it provides a greater increase in productivity, which may result in a greater financial return to the producer.

RESUMO: Este trabalho teve como objetivo avaliar as qualidades física e fisiológica, bem como a produtividade das sementes de milho, variedade UFVM 100 Nativo, produzidas em lavouras adubadas com diferentes níveis de resíduo avícola, em cobertura. O experimento foi instalado no delineamento em blocos casualizados, com seis tratamentos e quatro repetições, totalizando 24 parcelas. Os tratamentos consistiram da aplicação de 0,0; 1,5; 3,0; 4,5; 6,0 e 7,5 t/ha de resíduo avícola curtido. As sementes foram submetidas às seguintes avaliações: Teste de uniformidade, peso de mil sementes, germinação, vigor (primeira contagem de germinação, envelhecimento acelerado, teste de frio sem solo, porcentagem e velocidade de emergência em areia) e produtividade. Para avaliar o efeito das diferentes dosagens de resíduo avícola sobre as variáveis consideradas foram utilizados modelos de regressão linear. A qualidade das sementes não foi influenciada pelo efeito dos diferentes níveis de adubação. Dentre as dosagens utilizadas, recomenda-se a de 7,5 t/ha por proporcionar maior aumento da produtividade, o que poderá resultar em maior retorno financeiro ao produtor.

Physical and physiological qualities and productivity of corn seeds fertilized with poultry waste / Avaliação das qualidades física e fisiológica e da produtividade de sementes de milho adubado com resíduo avícola

This study evaluated the physical and physiological qualities and productivity of corn seeds, a variety UFVM 100 Native, produced in plots fertilized in top dressing with different levels of poultry waste. The experiment was organized in a randomized block design, with six treatments and four repetitions, totaling 24 plots. The treatments consisted of the application of 0.0, 1.5, 3.0, 4.5, 6.0, and 7.5 t/ha of tanned poultry waste. The seeds were subjected to the following evaluations: uniformity test, thousand seed weight, germination, vigor (first germination count, accelerated aging, soilless cold test, percentage, and emergence rate in sand), and productivity. Linear regression models were used to evaluate the effects of different dosages of poultry waste on the variables considered. The quality of seeds was not influenced by the effects of different levels of fertilization. Among the dosages used, 7.5 t/ha is recommended because it provides a greater increase in productivity, which may result in a greater financial return to the producer.

Este trabalho teve como objetivo avaliar as qualidades física e fisiológica, bem como a produtividade das sementes de milho, variedade UFVM 100 Nativo, produzidas em lavouras adubadas com diferentes níveis de resíduo avícola, em cobertura. O experimento foi instalado no delineamento em blocos casualizados, com seis tratamentos e quatro repetições, totalizando 24 parcelas. Os tratamentos consistiram da aplicação de 0,0; 1,5; 3,0; 4,5; 6,0 e 7,5 t/ha de resíduo avícola curtido. As sementes foram submetidas às seguintes avaliações: Teste de uniformidade, peso de mil sementes, germinação, vigor (primeira contagem de germinação, envelhecimento acelerado, teste de frio sem solo, porcentagem e velocidade de emergência em areia) e produtividade. Para avaliar o efeito das diferentes dosagens de resíduo avícola sobre as variáveis consideradas foram utilizados modelos de regressão linear. A qualidade das sementes não foi influenciada pelo efeito dos diferentes níveis de adubação. Dentre as dosagens utilizadas, recomenda-se a de 7,5 t/ha por proporcionar maior aumento da produtividade, o que poderá resultar em maior retorno financeiro ao produtor.

Performance, physical egg quality, and economic index of laying hens under different rearing systems / Desempenho, qualidade do ovo e índice econômico de poedeiras em diferentes sistemas de criação

The objective was to evaluate the performance, physical egg quality (weight and gravity), and economic index of laying hens reared in cage and cage-free systems. A total of 312 40-week-old Hy-line brown laying hens were distributed in a fully randomized design, split into two treatments (cage-free and cage) with 12 replicates in each system. We housed 144 birds in galvanized-wire cages (12 birds each) and 168 birds in a cage-free system in boxes with wood shaving bedding (14 birds each). The experiment lasted 112 days and divided into four 28-day periods. Regarding performance, feed intake (g) and feed conversion (g/g) of birds reared in the cage-free system were higher (p<0.0001) than the cage system, but no differences were observed (p>0.05) for the other performance parameters and also for egg quality. As for economic index, egg production in the cage-free system was higher (p<0.0001) than the cage system. In conclusion, birds reared in the cage-free system have higher feed consumption but worse feed conversion, increasing egg production cost compared to caged birds; however, there are no changes in egg production and physical quality.(AU)

Objetivou-se avaliar o desempenho, qualidade de ovos e índice econômico de poedeiras semipesadas criadas em sistemas cage-free e em gaiolas. Foram utilizadas 312 poedeiras da linhagem Hy Line Brown, com 40 semanas de idade, distribuídas em um delineamento inteiramente casualisado (DIC), em dois tratamentos (cage-free e gaiolas) com 12 repetições em cada sistema, sendo 144 aves alojadas em gaiolas de arame galvanizado com 12 aves em cada gaiola e 168 aves alojadas em sistema cage-free, em boxes sobre cama de maravalha com 14 aves em cada repetição. O experimento teve duração de 112 dias, divididos em quatro períodos de 28 dias. Nos parâmetros de desempenho, o consumo de ração (g/aves/dia) e a conversão alimentar (g/g) foi maior (P<0,0001) nas aves criadas no sistema cage-free comparado ao sistema de gaiolas, não sendo observado diferença (P>0,05) nos demais parâmetros de desempenho e também na qualidade de ovos. No índice econômico, a produção de ovos em sistema cage-free apresentou maior valor (P<0,0001) em relação ao sistema de gaiola. Conclui-se que as aves criadas em sistema cage-free apresentam maior consumo de ração, pior conversão alimentar ocasionando um maior custo nos ovos em relação às aves criadas em gaiolas, no entanto não há alteração na produção e qualidade dos ovos.(AU)

Stable fly outbreaks in Brazil: a 50-year (1971-2020) retrospective / Surtos da mosca-dos-estábulos no Brasil: Retrospectiva 50 anos (1971-2020)

Over the last decades, stable fly (Stomoxys calcitrans) outbreaks associated with agricultural and/or livestock production systems have become a serious problem in some Brazilian locations. This article presents a survey on the history, evolution and mapping of such outbreaks in Brazil over five decades (1971-2020). Outbreaks (n= 579) were recorded in 285 municipalities from 14 states, mainly associated with by-products from the ethanol industry (82.7%), in natura organic fertilizers (12.6%) and integrated crop-livestock systems (3.1%). Few cases were reported until the mid-2000s, progressively increasing since then. Outbreaks associated with ethanol mills occurred in 224 municipalities, mainly in Southeast and Midwest states, while those associated with organic fertilizers (mainly poultry litter and coffee mulch) affected 39 municipalities, mostly in the Northeast and Southeast states. More recently, outbreaks in integrated crop-livestock systems during the rainy season have occurred in Midwest states. This survey highlights the magnitude of the problem of stable fly outbreaks in Brazil and its relationship with environmental public policies, agricultural production chains and regional trends. Specific public actions and policies are urgently needed to prevent their occurrence and impact in the affected regions.(AU)

Nas últimas décadas, surtos da mosca-dos-estábulos (Stomoxys calcitrans), associados a sistemas de produção agrícola e/ou pecuária, tornaram-se um grave problema em algumas localidades brasileiras. Este artigo apresenta um levantamento sobre a história, evolução e mapeamento desses surtos no Brasil ao longo de cinco décadas (1971-2020). Surtos (n= 579) foram registrados em 285 municípios de 14 estados, principalmente associados a subprodutos da indústria do etanol (82,7%), fertilizantes orgânicos in natura (12,6%) e sistemas de integração lavoura-pecuária (3,1%). Poucos casos foram registrados até meados dos anos 2000, aumentando progressivamente desde então. Surtos associados a usinas de etanol ocorreram em 224 municípios, principalmente no Sudeste e Centro-Oeste, enquanto surtos associados a fertilizantes orgânicos (principalmente esterco aviário e palha de café) afetaram 39 municípios, principalmente nos estados do Nordeste e Sudeste. Mais recentemente, surtos em sistemas de integração lavoura-pecuária, durante a estação chuvosa, têm ocorrido no Centro-Oeste. Este levantamento ressalta a magnitude do problema dos surtos da mosca-dos-estábulos no Brasil e sua relação com políticas públicas ambientais, cadeias produtivas agrícolas e tendências regionais. Ações e políticas públicas específicas são urgentemente necessárias para prevenir sua ocorrência e impacto nas regiões afetadas.(AU)

Efficacy of Aluminum Sulfate Addition to Poultry Litter on Productive Performance of Laying Hens, Ammonia Emissions, and Litter Quality

The objective of this research was to examine whether different types of litter (New, used, and mixed) and alum could interfere in litter quality, thereby also influencing the productive performance of laying hens. A total number of 450 Inshas chickens aged 24 weeks were randomly distributed into six groups (5 replicates, each with 15 hens) of floor litter. The groups included (G1) new wheat straw litter, (G2) used wheat straw litter, (G3) mixed wheat straw litter (50% new+50% used) and (4, 5 and 6) the same litter types in groups 1, 2 and 3 supplied with 500g of alum /m2 litter. The experiment lasted 16 weeks. Laying hens productive parameters, blood parameters, carcass parameters, respiratory rate, coliform count and pH, as well as ammonia emission, litter quality were assessed.The results showed that the litter type significantly affects the level of ammonia emission, litter characteristics, carcass characteristics, intestinal pH and coliform count, blood characteristics (T3, cholesterol, uric acid, total protein, albumin, globulin, hemoglobin, and PCV), as well as the body weight gain. In general, we found that reused litter followed by the mixed litter without added alum resulted in the lowest efficiency on the studied traits, as compared to the same types of litter treated with alum and the new litter. We therefore conclude that the addition of alum reduces ammonia emission and improves the characteristics of the litter, which is reflected in enhancement of productive performance and properties of blood and carcass.(AU)

Ammonia gas for bacterial control in poultry litter

ABSTRACT: The current techniques used in the disinfection of reused poultry litter, such as lime addition, windrowing and plastic cover on the surface, do not guarantee the elimination of pathogenic microorganisms, causing damage to the environment and animal health. Gram negative bacteria, i.e., Salmonella and Escherichia coli, can be transmitted from one batch to another through reused litter, causing health damage to broilers and humans that consume food contaminated by these agents. Our study assessed the effectiveness of the methods plastic cover on the surface (PCS) and plastic cover on the surface with ammonia gas injection (PCSAI) in the control of Gram negative bacteria. The results obtained, both in laboratory conditions (Experiment 1) and in the field (Experiment 2), demonstrate that the method PCSAI with 0.22% ammonia gas had a significant reduction (P 0.05) of Gram negative bacteria in the period of 48 hours This new methodology for disinfecting poultry litter will allow its reuse in a practical and safe way, improving the preservation of the environment, of the health of broilers and consumers of poultry products.

RESUMO: O reaproveitamento de camas aviárias na criação de frangos de corte é uma prática muito utilizada no Brasil. Essa prática reduz custos de produção e contribui na conservação do meio ambiente. As técnicas atuais utilizadas na desinfecção de camas aviárias reaproveitadas, como adição de cal, enleiramento e lona na superfície, não garantem a eliminação de microrganismos patogênicos porque não geram quantidade suficiente de amônia. O gás amônia, em concentrações elevadas, tem efeito biocida. Bactérias Gram negativas, como as Salmonelas e Escherichia coli, podem ser transmitidas de um lote para outro através do reaproveitamento da cama, ocasionando prejuízos para a saúde das aves e dos humanos que consomem alimentos contaminados por estes agentes. Este trabalho avaliou a eficácia do método lona na superfície com injeção de gás amônia no controle de microrganismos Gram negativos. Os resultados obtidos demostraram que esse método controlou os microrganismos Gram negativos num período de 48 horas em camas de frangos de corte reaproveitadas. Assim, essa nova metodologia de desinfecção de camas de aviário permitirá sua reutilização de forma prática e segura, melhorando a saúde das aves e dos consumidores dos produtos avícolas.

Ammonia gas for bacterial control in poultry litter / Gás amônia para controle bacteriano em cama aviária

The current techniques used in the disinfection of reused poultry litter, such as lime addition, windrowing and plastic cover on the surface, do not guarantee the elimination of pathogenic microorganisms, causing damage to the environment and animal health. Gram negative bacteria, i.e., Salmonella and Escherichia coli, can be transmitted from one batch to another through reused litter, causing health damage to broilers and humans that consume food contaminated by these agents. Our study assessed the effectiveness of the methods plastic cover on the surface (PCS) and plastic cover on the surface with ammonia gas injection (PCSAI) in the control of Gram negative bacteria. The results obtained, both in laboratory conditions (Experiment 1) and in the field (Experiment 2), demonstrate that the method PCSAI with 0.22% ammonia gas had a significant reduction (P<0.05) of Gram negative bacteria in the period of 48 hours This new methodology for disinfecting poultry litter will allow its reuse in a practical and safe way, improving the preservation of the environment, of the health of broilers and consumers of poultry products.

O reaproveitamento de camas aviárias na criação de frangos de corte é uma prática muito utilizada no Brasil. Essa prática reduz custos de produção e contribui na conservação do meio ambiente. As técnicas atuais utilizadas na desinfecção de camas aviárias reaproveitadas, como adição de cal, enleiramento e lona na superfície, não garantem a eliminação de microrganismos patogênicos porque não geram quantidade suficiente de amônia. O gás amônia, em concentrações elevadas, tem efeito biocida. Bactérias Gram negativas, como as Salmonelas e Escherichia coli, podem ser transmitidas de um lote para outro através do reaproveitamento da cama, ocasionando prejuízos para a saúde das aves e dos humanos que consomem alimentos contaminados por estes agentes. Este trabalho avaliou a eficácia do método lona na superfície com injeção de gás amônia no controle de microrganismos Gram negativos. Os resultados obtidos demostraram que esse método controlou os microrganismos Gram negativos num período de 48 horas em camas de frangos de corte reaproveitadas. Assim, essa nova metodologia de desinfecção de camas de aviário permitirá sua reutilização de forma prática e segura, melhorando a saúde das aves e dos consumidores dos produtos avícolas.

Activity of powdered methylxanthine applied to poultry litter on adults of Alphitobius diaperinus (Panzer, 1797) (Coleoptera: Tenebrionidae) / Atividade da Metilxantina em pó aplicada na cama de frango sobre adultos de Alphitobius diaperinus (Panzer, 1797) (Coleoptera: Tenebrionidae)

One of the pests that most affect and compromise poultry production worldwide is the insect Alphitobius diaperinus, known as the lesser mealworm. This insect is a vector of diseases that compromise not only chicken production but also human health. This study proposes to examine the efficacy and determine the appropriate rate of methylxanthine (MTX), a natural insecticide extracted from cafeine, for the control of an adult population of lesser mealworms in poultry litter. A total of 2,500 adult mealworms were distributed into five treatments in a completely randomized design using 10 replications with 50 insects per replication. The treatments consisted of a control group and four concentrations of MTX (14, 16, 18, and 20 g/m2) spread in plastic boxes containing reused poultry litter and feed, allocated in a broiler shed, to simulate the farm condition. The experimental period was 18 days, and five readings were performed on days 2, 4, 6, 10, and 18. Methylxanthine affected (P<0.05) the mealworms' cumulative mortality rate, with the groups of insects housed in boxes treated with 16 g /m2 MTX showing the highest cumulative mortality (86.6%) at the end of the experimental period. In conclusion, MTX has insecticidal action on adults of lesser mealworm and can be used on chicken litter to control the population of this insect in poultry sheds. The MTX concentration of 16 g/m2 showed the greatest effectiveness.

Uma das pragas que mais afetam e comprometem a produção avícola no mundo é o inseto Alphitobius diaperinus, conhecido como cascudinho. Este inseto é vetor de doenças que comprometem não só a produção de frangos como também a saúde humana. Objetivou-se com esse trabalho avaliar a eficácia e determinar a dose adequada de Metilxantina (MTX), inseticida natural extraído da cafeína, para o controle da população adulta de cascudinhos em cama de frango. Foram utilizados 2.500 cascudinhos adultos distribuídos em delineamento inteiramente casualizado, cinco tratamentos, 10 repetições com 50 insetos por repetição. Os tratamentos consistiram de grupo controle e quatro concentrações 14 g/m2, 16 g/m2, 18 g/m2, 20 g/m2 de MTX espalhadas em recipientes plásticos contendo cama de frango reutilizada e ração, alocados em um galpão de frangos de corte a fm de simular a condição de granja. Período experimental foi de 18 dias e realizadas cinco leituras nos dias dois, quatro, seis, 10 e 18. A MTX afetou (P<0,05) a taxa de mortalidade acumulada de cascudinhos, grupos de insetos alojados em caixas tratadas com 16 g/m2 de MTX apresentaram maior mortalidade acumulada (86,6%) ao final do período experimental. Conclui-se que MTX tem ação inseticida sobre adultos de cascudinho, podendo ser utilizada sobre a cama de frango para o controle da população deste inseto em galpões de criação de frangos, a concentração 16 g/MTX/m2 demonstrou maior efetividade.

Características físico-químicas da cama de aviário e desempenho de frangos de corte alojados em diferentes materiais de cama e duas densidades

La arena para aviarios se utiliza para absorber la humedad y convertirse en una barrera para el contacto directo del ave con el suelo en los sistemas de cría intensiva. De esta forma, contribuye al bienestar y mejor desempeño productivo de las aves al reducir los cambios bruscos de temperatura en los galpones. El objetivo de este trabajo fue evaluar las características físicas y químicas de cuatro tipos de material de cama bajo dos densidades de alojamiento y el comportamiento de pollos de engorde alojados en diferentes tratamientos durante un período de 42 días. Se adoptó un diseño experimental completamente al azar, en un esquema factorial 4 x 2 (cuatro tipos de material de cama y dos tasas de carga) y cinco repeticiones. Los cuatro tipos de material de cama fueron arena lavada, virutas de madera, cáscara de arroz y heno Tifton 85 y las dos tasas de carga fueron de 10 y 14 aves/m2. Los parámetros de desempeño de los animales evaluados fueron: aumento de peso, consumo, conversión alimenticia, peso vivo y viabilidad, mientras que las características analizadas de la camada fueron: pH, temperatura y humedad. Los materiales utilizados para la cama de aves de corral no afectaron negativamente el rendimiento de las aves, sin embargo, las aves alojadas a una densidad de 10 aves/m2 tuvieron un mejor rendimiento. No hubo diferencia entre los valores de pH y temperatura de los diferent

Aviary litter is used to absorb moisture and become a barrier to the bird's direct contact with the ground in intensive rearing systems. In this way, it contributes to the welfare and better productive performance of birds by reducing sudden temperature changes in the sheds. The objective of this work was to evaluate the physical and chemical characteristics of four types of litter material under two housing densities and the performance of broilers housed in different treatments over a period of 42 days. A completely randomized experimental design was adopted, in a 4 x 2 factorial scheme (four types of litter material and two stocking rates) and five replications. The four types of litter material were washed sand, wood shavings, rice husk and Tifton 85 hay and the two stocking rates were 10 and 14 birds/m2. The performance parameters of the evaluated animals were: weight gain, consumption, feed conversion, live weight and viability, while the analyzed characteristics of the litter were: pH, temperature and humidity. The used poultry litter materials did not negatively affect the performance of the birds, however the birds housed at a density of 10 birds/m2 had better performance. There was no difference between the pH and temperature values of the different treatments, however the humidity of the sand bed at a density of 10 birds/m2 was significantly higher than that of the o

A cama de aviário é utilizada para absorver umidade e se tornar uma barreira do contato direto da ave com o chão em sistemas intensivos de criação. Desse modo, contribui para o bem-estar e melhor desempenho produtivo das aves por reduzir as mudanças bruscas de temperatura nos galpões. O objetivo deste trabalho foi avaliar as características físico-químicas de quatro tipos de material de cama aviária sob duas densidades de alojamento e o desempenho de frangos de corte alojados nos diferentes tratamentos no período de 42 dias. Foi adotado o delineamento experimental inteiramente ao acaso, em um esquema fatorial 4 x 2 (quatro tipos de material de cama e duas taxas de lotação) e cinco repetições. Os quatro tipos de material de cama foram areia lavada, maravalha, casca de arroz e feno de Tifton 85 e as duas taxas de lotação foram 10 e 14 aves/m2. Os parâmetros de desempenho dos animais avaliados foram: ganho de peso, consumo, conversão alimentar, peso vivo e viabilidade, enquanto que as características analisadas da cama foram: pH, temperatura e umidade. Os materiais de cama aviária utilizados não afetaram negativamente o desempenho das aves, entretanto as aves alojadas na densidade de 10 aves/m2 apresentam melhor desempenho. Não houve diferença entre os valores de pH e temperatura dos diferentes tratamentos, entretanto a umidade da cama de areia na densidade de 10 aves/m2 foi signi

Características físico-químicas da cama de aviário e desempenho de frangos de corte alojados em diferentes materiais de cama e duas densidades / Physical-chemical characteristics of poultry litter and performance of broilers housed in different litter materials and two densities / Características físico-químicas de la cama de aves de corral y rendimiento de los pollos de engorde alojados en diferentes materiales de cama y dos densidades

A cama de aviário é utilizada para absorver umidade e se tornar uma barreira do contato direto da ave com o chão em sistemas intensivos de criação. Desse modo, contribui para o bem-estar e melhor desempenho produtivo das aves por reduzir as mudanças bruscas de temperatura nos galpões. O objetivo deste trabalho foi avaliar as características fisico-químicas de quatro tipos de material de cama aviária sob duas densidades de alojamento e o desempenho de frangos de corte alojados nos diferentes tratamentos no período de 42 dias. Foi adotado o delineamento experimental inteiramente ao acaso, em um esquema fatorial 4 x 2 (quatro tipos de material de cama e duas taxas de lotação) e cinco repetições. Os quatro tipos de material de cama foram areia lavada, maravalha, casca de arroz e feno de Tifton 85 e as duas taxas de lotação foram 10 e 14 aves/m2. Os parâmetros de desempenho dos animais avaliados foram: ganho de peso, consumo, conversão alimentar, peso vivo e viabilidade, enquanto que as características analisadas da cama foram: pH, temperatura e umidade. Os materiais de cama aviária utilizados não afetaram negativamente o desempenho das aves, entretanto as aves alojadas na densidade de 10 aves/m² apresentam melhor desempenho. Não houve diferença entre os valores de pH e temperatura dos diferentes tratamentos, entretanto a umidade da cama de areia na densidade de 10 aves/m² foi significativamente maior que a dos demais materiais. Portanto, os materiais de cama aviária utilizados nesse estudo apresentaram características físico-químicas semelhantes, sendo que a areia lavada tem maior poder de absorção de água, mas, mesmo assim, não afetou negativamente o desempenho das aves. As aves alojadas na densidade de 10 aves/m² apresentaram melhor desempenho.

Aviary litter is used to absorb moisture and become barrier to the bird's direct contact with the ground in intensive rearing systems. In this way, it contributes to the welfare and better productive performance of birds by reducing sudden temperature changes in the sheds. The objective of this work was to evaluate the physical and chemical characteristics of four types of litter material under two housing densities and the performance of broilers housed in different treatments over a period of 42 days. A completely randomized experimental design was adopted, in a 4 x 2 factorial scheme (four types of litter material and two stocking rates) and five replications. The four types of litter material were washed sand, wood shavings, rice husk and Tifton 85 hay and the two stocking rates were 10 and 14 birds/m². The performance parameters of the evaluated animals were: weight gain, consumption, feed conversion, live weight and viability, while the analyzed characteristics of the litter were: pH, temperature and humidity. The used poultry litter materials did not negatively affect the performance of the birds, however the birds housed at a density of 10 birds/m² had better performance. There was no difference between the pH and temperature values of the different treatments, however the humidity of the sand bed at a density of 10 birds/m² was significantly higher than that of the other materials. Therefore, the avian litter materials used in this study had similar physicochemical characteristics, with washed sand having greater water absorption power, but even so, it did not negatively affect the performance of the birds. Poultry housed at a density of 10 birds/m2 showed better performance.

La arena para aviarios se utiliza para absorber la humedad y convertirse en una barrera para el contacto directo del ave con el suelo en los sistemas de cría intensiva. De esta forma, contribuye al bienestar y mejor desempeño productivo de las aves al reducir los cambios bruscos de temperatura en los galpones. El objetivo de este trabajo fue evaluar las características físicas y químicas de cuatro tipos de material de cama bajo dos densidades de alojamiento y el comportamiento de pollos de engorde alojados en diferentes tratamientos durante un período de 42 días. Se adoptó un diseño experimental completamente al azar, en un esquema factorial 4 x 2 (cuatro tipos de material de cama y dos tasas de carga) y cinco repeticiones. Los cuatro tipos de material de cama fueron arena lavada, virutas de madera, cáscara de arroz y heno Tifton 85 y las dos tasas de carga fueron de 10 y 14 aves/m². Los parámetros de desempeño de los animales evaluados fueron: aumento de peso, consumo, conversión alimenticia, peso vivo y viabilidad, mientras que las características analizadas de la camada fueron: pH, temperatura y humedad. Los materiales utilizados para la cama de aves de corral no afectaron negativamente el rendimiento de las aves, sin embargo, las aves alojadas a una densidad de 10 aves/m² tuvieron un mejor rendimiento. No hubo diferencia entre los valores de pH y temperatura de los diferentes tratamientos, sin embargo la humedad del lecho de arena a una densidad de 10 aves/m² fue significativamente mayor que la de los otros materiales. Por lo tanto, los materiales de cama de aves utilizados en este estudio tenían características fisicoquímicas similares, teniendo la arena lavada un mayor poder de absorción de agua, pero aun así, no afectó negativamente el desempeño de las aves. Las aves de corral alojadas a una densidad de 10 aves/m² mostraron un mejor rendimiento.

The Use of A Compound Based on Phyllosilicates and Cinnamon Essential Oil for Chicken Broiler's Litter

The objective of this study was to evaluate the effectiveness of a litter conditioning compound (LCC) based on 98% phyllosilicates (kaolinite) and 2% cinnamon essential oil to be applied on the litter of broiler houses. Animal performance, prevalence of footpad lesions, and litter quality (water activity, pH, and moisture) were evaluated, in addition to the effect on the incidence of Salmonella spp., evaluated by testing shoe-drag swabs, fecal samples, and organs. Twenty-one broiler houses with similar structure and rearing conditions were distributed in a completely randomized design with three treatments and seven replications each, as follows: T0: control, without the use of LCC; T100: application of 100 grams of LCC per m² of litter per week; T200: application of 200 grams of LCC per m² of litter per week. There was no significant effect of treatments on performance variables; however litter pH was lower when 200g/m2 was used in comparison with the control treatment, a result not verified for moisture and water activity (p>0.05). Our findings demonstrated significant reduction in the percentage of footpad lesions in birds that received the LCC (T0: 63.37%; T100: 41.38% and T200: 27.24%). A reduction in the number of positive flocks for Salmonella spp. Was also observed (with overall positivity rates of 17.86%, 12.14%, and 5% for treatments T0, T100, and T200, respectively). It is concluded that the product reduces the incidence of Salmonella spp. and also significantly reduces the incidence of footpad lesions, two important factors for the poultry industry.(AU)

Liquid preparation of diatomaceous earth against the lesser mealworm, Alphitobius diaperinus (Panzer, 1797) (Coleoptera: Tenebrionidae)

The lesser mealworm (LMW) is the most important pest in poultry production. Insects are associated to avian pathogens, mainly Salmonella. Its control is based on chemical insecticide, with limited efficacy in population reduction. Also, insect populations are resistant to the main active ingredients used. Diatomaceous earth (DE) is a mineral dust that has activity against the LMW when used in dust application. No information is available about DE liquid preparation against this insect. Thus, bioassays were conducted aiming to develop a strategy for DE liquid preparation to this insect control. In laboratory the ideal concentration for poultry house experiment was estimated and the effect of insect contact with poultry litter or chicken feed after application was checked. In the poultry house, DE liquid preparation (10% in water, 1 L·m­2) was applied on the dirt soil of a cleaned and empty poultry house. In the control poultry house, a chemical insecticide was used (cypermethrin 15 g + chlorpyriphos 25 g + piperonyl butoxide 15 g) (1 L·m­2). DE at all concentrations was efficient mainly when insects were dried for 24 h before contact with a substrate. In poultry house, both treatments obtained similar results at 15 days after treatment (94% of reduction of insect population). At 45 days after treatment, the insect population with DE treatment increased 39% while the insect population remained 17% smaller than the initial population with a chemical insecticide. DE liquid preparation has potential to be used as a safe treatment in LMW population management, as a shock treatment.

Ammonia levels during broiler rearing cycle

The evaluated ammonia volatilization rates and its association with humidity, temperature, and pH in broiler litter in conventional and dark house rearing systems. Evaluations were performed at 0.01, 1.00, and 1.50 m high, using a completely randomized design, at a weekly frequency for 12 weeks­from the sanitary void to the end of the 42-day cycle. Litter temperature had no significant difference for different types of house. Humidity and pH levels varied according to houses and evaluation periods. No ammonia volatilization was detected during initial periods. However, up from the 21st day of rearing, rates started to increase, showing higher concentrations at 0.01 m height. At 1.00 and 1.50 m, values around 20 mL·m­3, were obtained and considered an acceptable exposure level for the broilers and employees directly working in this sector. Yet, broiler breeders should target lower levels due to environmental impacts.

Poultry litter biochar rates and incubation times: effects on soil fertility and radish development / Doses de biocarvão de cama de aviário e tempo de incubação: efeitos na fertilidade do solo e desenvolvimento de rabanete

The objective of this study was to evaluate the effect of increasing rates of poultry litter biochar incubated in soil for different periods on soil fertility attributes and the development of the radish plant. An experiment was carried out in a greenhouse at the Federal University of Campina Grande - UFCG. Treatments consisted of the combination of two factors: four incubation times (0, 30, 60, and 90 days) and four biochar rates (12.5, 25.0, 37.5, 50.0 t ha-1), in three replications, totalizing 48 experimental plots, in a completely randomized design. After the incubation period, soil samples were collected from each experimental unit and chemically characterized. Following this, radish was sown and cultivated for up to 30 days. The agronomic development of radish was evaluated based on the following variables: leaf area; leaf area ratio; specific leaf area; shoot biomass production index; transverse and longitudinal tuber diameters; and fresh and dry tuber biomasses. Under the present experimental conditions, biochar increases soil fertility levels and is able to supply nutrients to the plants in a short period, increasing plant variables.(AU)

O objetivo deste trabalho foi avaliar o efeito de doses crescentes de biocarvão de cama de aviário, incubado ao solo por diferentes períodos, nos atributos de fertilidade do solo e no desenvolvimento de rabanete. Para tanto, foi realizado um experimento em casa de vegetação, na Universidade Federal de Campina Grande - UFCG, onde os tratamentos resultaram da combinação de dois fatores: 4 tempos de incubação (0, 30, 60 e 90 dias) e 4 doses de biocarvão (12,5, 25,0, 37,5, 50,0 t ha-1) com três repetições, totalizando 48 unidades experimentais, em delineamento inteiramente casualizado. Após o período de incubação, foram coletadas amostras de solo de cada unidade experimental, caracterizadas quimicamente e em seguida o rabanete foi semeado e cultivado por até 30 dias. O desenvolvimento agronômico do rabanete foi avaliado conforme as variáveis: área foliar; razão de área foliar; área foliar específica e índice de produção de biomassa da parte aérea do rabanete; diâmetro transversal e longitudinal do rabanete; biomassa fresca e seca do rabanete. Pelas condições experimentais, concluiu-se que o biocarvão aumentou os níveis de fertilidade do solo e foi capaz de fornecer nutrientes às plantas em um curto período aumentando as variáveis analisadas das plantas.(AU)

Effects of Medium Chain Fatty Acid Supplementation in Broiler Diet on Microbiological Quality of Litter

In our experiment, coconut and palm oil supplementation was added to the diet of broiler chickens to prove the hypothesis that medium chain fatty acids (MCFAs) can reduce the occurrence of potential pathogens (Clostridium perfingens, Coliform) in gut microflore and therefore improve bird welfare. Cobb 500 cockerels were divided in five groups. Control birds were fed commercial broiler diet, while birds in the four treatment group diets were supplemented with coconut, palm oil, or a combination of the two, respectively. As a positive control, sunflower oil supplementation was included in the diet of the fifth group. During the 28 days of the study (from 14 to 42 days of life), 5 samplings were scheduled, when excreta samples were taken from the litter to analyse total microbial count and the number of Clostridia, Coliforms, and Salmonella. According to the results of microbiological analysis, coconut oil supplementation led to the continuous decline of Clostridium perfringens numbers until they vanished by the end of the experimental period. A similar but faster decline was found as a result of palm oil and the combined (palm oil+coconut oil) treatment. However, the number of Coliforms and total microbial count changed only slightly by the end of the study. No Salmonella spp. was present in the samples throughout the experiment. Altogether, coconut and palm oil supplementation has a beneficial effect on the microbiological composition of poultry litter.(AU)

Effects of Medium Chain Fatty Acid Supplementation in Broiler Diet on Microbiological Quality of Litter

In our experiment, coconut and palm oil supplementation was added to the diet of broiler chickens to prove the hypothesis that medium chain fatty acids (MCFAs) can reduce the occurrence of potential pathogens (Clostridium perfingens, Coliform) in gut microflore and therefore improve bird welfare. Cobb 500 cockerels were divided in five groups. Control birds were fed commercial broiler diet, while birds in the four treatment group diets were supplemented with coconut, palm oil, or a combination of the two, respectively. As a positive control, sunflower oil supplementation was included in the diet of the fifth group. During the 28 days of the study (from 14 to 42 days of life), 5 samplings were scheduled, when excreta samples were taken from the litter to analyse total microbial count and the number of Clostridia, Coliforms, and Salmonella. According to the results of microbiological analysis, coconut oil supplementation led to the continuous decline of Clostridium perfringens numbers until they vanished by the end of the experimental period. A similar but faster decline was found as a result of palm oil and the combined (palm oil+coconut oil) treatment. However, the number of Coliforms and total microbial count changed only slightly by the end of the study. No Salmonella spp. was present in the samples throughout the experiment. Altogether, coconut and palm oil supplementation has a beneficial effect on the microbiological composition of poultry litter.

Evaluation of Analytical Sensitivity of Sdf I based PCR and Sandwich ELISA for Salmonella Enteritidis detection and On-Farm prevalence in Punjab, Pakistan

Salmonella Enteritidis (SE) is a dominant serotype among non-typhoidal Salmonella which renders poultry products unsafe for human consumption. Due to frequent reporting of egg associated outbreaks, broiler breeder flocks are understudied although farm environment present supporting conditions for the growth of SE. In this study, two rapid detection techniques for SE were compared in terms of analytical sensitivity and the extent of SE contamination in broiler breeder farm environment was determined. Analytical sensitivity as limit of detection (LOD) was evaluated quantitatively for serotype specific PCR based on amplification of Sdf I gene and a commercially available sandwich ELISA for antigen detection. In triplicate experiments, tenfold serial dilutions of SE were prepared and tested with each technique. Using pure cultures, analytical sensitivity of PCR and ELISA were found to be 18.6 CFU/ml and 2.77×105 CFU/ml respectively. PCR (LOD, log 1.2) was found to be more sensitive and rapid than ELISA (LOD, log 5.4). Environmental swab samples (n = 260) were collected from 22 hen houses representing 8 broiler breeder farms located in and around Lahore and Sheikhupura districts of Punjab province. From each hen house swab samples were collected from litter, nests, feeders, drinkers, fans, pads, ceiling, walls and walkways. Following selective enrichment, pooled swab samples were subjected to PCR. Results showed that 36.3 % (8/22) hen houses were detected positive for SE. These findings suggest improvement in farm biosecurity measures and advocate implementation of integrated Salmonellosis control programs in broiler breeder houses to minimize carcass contamination.(AU)

Symptoms of deficiency and initial growth of maize cultivated with biochar under nutrient omission / Sintomas de deficiência e crescimento inicial do milho cultivado com biocarvão sob omissão de nutrientes

Maize is the second largest agricultural crop in Brazil. It reaches high yields as supported by the intensive use of technologies, particularly mineral fertilization, which is normally costly. To lower production costs and improve crop productivity on small farms, the present study tested the efficiency of poultry litter biochar as a source of nutrients in the initial growth of BRS 2022 maize by the 'diagnosis by subtraction' method. The study was carried out in a greenhouse, using a completely randomized experimental design with a factorial arrangement (7×3). The following treatments were tested: complete nutrient solution (N, P, K, Ca, Mg, S, B, Cl, Cu, Fe, Mn, and Zn); complete nutrient solutions with omission of only nitrogen (-N), phosphorus (-P), potassium (-K), calcium (-Ca), and magnesium (-Mg); and complete absence of nutrients and three increasing rates of biochar (0, 5, and 10 t ha-1). Absence of nutrients with biochar rates significantly influenced the growth and dry biomass production variables of the maize plants. Except for stem diameter and the ratio between shoot and root dry biomass, all variables were influenced by the interaction between nutrients and biochar rates. Nutrient omission limited maize growth; however, the application of biochar reduced these limitations and significantly improved all analyzed variables. In the treatments without fertilizer, maize growth was very low, with generalized symptoms of deficiency that would decrease with the application of biochar. Even in the treatment with complete fertilization, which showed some slight visual symptoms, these decreased with the application of biochar.(AU)

A cultura do milho é a segunda maior produção agrícola do Brasil. Alcança altos rendimentos, apoiada no uso intensivo de tecnologias, em particular, a adubação mineral, normalmente onerosa. Na visão de baixar custos de produção e melhorar a produtividade da cultura na pequena propriedade rural, neste trabalho foi testado pelo método diagnose por subtração a eficiência do biocarvão da cama de frango como fonte de nutrientes ao crescimento inicial do milho BRS 2022. O estudo foi realizado em casa de vegetação, utilizando delineamento experimental inteiramente casualizado, em esquema fatorial (7x3), com os seguintes tratamentos: solução nutritiva completa (N, P, K, Ca, Mg, S, B, Cl, Cu, Fe, Mn, Zn); soluções nutricionais completas com a omissão de apenas nitrogênio (-N), fósforo (-P), potássio (-K), cálcio (-Ca) e magnésio (-Mg); com ausência completa de nutrientes e três doses crescentes de biochar (0, 5 e 10 t ha-1). As variáveis de crescimento e produção de biomassa seca de plantas de milho foram significativamente influenciadas pela ausência de nutrientes e pelo efeito das doses de biocarvão. Com exceção do diâmetro do caule e da relação entre a parte aérea e a biomassa da raiz seca as interações também foram significativamente afetadas pelos tratamentos. A omissão de nutrientes limitou o crescimento do milho, no entanto, a aplicação do biocarvão diminuiu essas limitações e melhorou significativamente todas as variáveis analisadas. Nos tratamentos sem adubação, o crescimento do milho foi muito baixo, com sintomas generalizados de deficiência que diminuiriam com a aplicação do biocarvão. Até mesmo o tratamento com adubação completa que apresentou alguns leves sintomas visuais, houve redução a aplicação do biocarvão.(AU)

Evaluation of the Ingestive Behavior of Foreign Fragments and the Integrity of Gastrointestinal Tract of Broiler Chickens

Fragments with vibrant colors and attractive textures in poultry house litter may be consumed considering that the confinement environment does not offer many opportunities for the development of the birdss natural behavior. Control and quality programs aiming to reduce and to eliminate the consumption of materials from the construction of sheds have been implanted. However, it is necessary to develop studies for a better understanding of the birds ingestive behavior and the consequences for their health, welfare and productivity. The goal of this study was to evaluate the ingestive behavior of broilers exposed to metal, wood, glass and plastic fragments at different concentrations and sizes added to the poultry house litter and the integrity of the gastrointestinal tract of broilers. A total of 360 birds were distributed in a completely randomized design over a 2x3 factorial scheme (2 fragment sizes: 2 to 7 mm and 5 to 8 mm and 3 concentrations: 0.1, 0.5 and 1%), totaling 6 treatments and 5 replicates. The fragments used were glass, wood, metal and plastic, in respective concentrations and sizes, distributed in the poultry house litter. The supply of fragments or the accidental occurrence of similar fragments to which were analyzed in this study concerning the poultry house litter, with different sizes, colors and textures does not compromise the integrity of the mucosa of the gastrointestinal tract and the welfare of the birds.(AU)