Evaluation and optimization of SYBR Green real-time reverse transcription polymerase chain reaction as a tool for diagnosis of the Flavivirus genus in Brazil.
Romeiro, Marilia Farignoli; Souza, William Marciel de; Tolardo, Aline Lavado; Vieira, Luiz Carlos; Colombo, Tatiana Elias; Aquino, Victor Hugo; Nogueira, Maurício Lacerda; Figueiredo, Luiz Tadeu Moraes.
Rev Soc Bras Med Trop
; 49(3): 279-85, 2016 May-Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27384823
Superiority of the buffy coat over serum or plasma for the detection of Alkhumra virus RNA using real time RT-PCR.
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Universal primers that amplify RNA from all three flavivirus subgroups.
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