Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells
Barreira, Luzia Aparecida Costa; Scheucher, Priscila Santos; Romeiro, Marilia Farignoli; Serra, Leonardo La; Badra, Soraya Jabur; Souza, William Marciel de; Figueiredo, Luiz Tadeu Moraes.
Rev. Soc. Bras. Med. Trop
; 51(2): 168-173, Mar.-Apr. 2018. graf
Artigo
em Inglês
| LILACS | ID: biblio-897058
Abstract
INTRODUCTION:
Dengue virus (DENV) is the most important arthropod-borne viral disease worldwide with an estimated 50 million infections occurring each year.METHODS:
In this study, we present a flow cytometry assay (FACS) for diagnosing DENV, and compare its results with those of the non-structural protein 1 (NS1) immunochromatographic assay and reverse transcriptase polymerase chain reaction (RT-PCR).RESULTS:
All three assays identified 29.1% (39/134) of the patients as dengue-positive. The FACS approach and real-time RT-PCR detected the DENV in 39 and 44 samples, respectively. On the other hand, the immunochromatographic assay detected the NS1 protein in 40.1% (56/134) of the patients. The Cohen's kappa coefficient analysis revealed a substantial agreement among the three methods.CONCLUSIONS:
The FACS approach may be a useful alternative for dengue diagnosis and can be implemented in public and private laboratories.
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