The aim of the present study was to describe an improved protocol of
reverse transcription polymerase chain reaction (RT-PCR) for
Yellow Fever virus genome detection. A
strain of
ribonucleic acid of
Yellow Fever virus was submitted to the improved protocol of RT-PCR and the amplicons were visualized under ultraviolet transilluminator, purifed and sequenced. The
nucleotide sequence obtained was compared with sequences available in
GenBank using the tblastx tool. The amplicons produced by the
strain of
ribonucleic acid of
Yellow Fever virus exhibited fragments of 400 and 800
base pairs and the
consensus sequence exhibited a similarity of 100% with
Yellow Fever virus sequences recorded in
GenBank. The improved protocol described in this study allowed
Yellow Fever virus genome detection and enabled the elimination of the nested-
PCR step, which has been frequently associated with
contamination. In addition, it reduced the
time of reaction, the
cost of
reagents and the possibility of sample
contamination. New
methods of investigating these
infections must be elaborated and a continuous vigilance of these
viruses in their diï¬erent vectors and hosts is required to avoid negative impacts on
human health,
tourism and trade.(AU)