Diagnosis of arboviruses using indirect sandwich IgG ELISA in horses from the Brazilian Amazon
Casseb, Alexandre do Rosário; Nunes, Márcio Roberto Teixeira; Rodrigues, Sueli Guerreiro; Travassos da Rosa, Elizabeth Salbé; Casseb, Lívia Medeiros Neves; Casseb, Samir Manssor Moraes; Silva, Sandro Patroca da; Rodrigues, Érika Dayane Leal; Vasconcelos, Pedro Fernando da Costa.
J. venom. anim. toxins incl. trop. dis
; 20(29): 1 - 4, July 3, 2014. mapas, tab
Artigo
em Inglês
| Instituto Evandro Chagas (SOPHIA) | ID: iec-14420
Background:
The Amazon as a whole is the largest reservoir of arboviruses worldwide, while the Brazilian Amazon hosts the largest variety of arboviruses isolated to date. In this study, the results of an indirect sandwich IgG ELISA, standardized for 19 arbovirustypes circulating among horses in Brazilian Amazon, were compared to results of the hemagglutination inhibition test. A screening test assessed the conditional probability distribution and a Pearson linear correlation test determined the correlation strength among the absorbance values recorded for viruses from the same family.Findings:
Sensitivity varied between 40.85 and 100 per cent; the specificity was low and ranged from 39.71 to 67.0 per cent; and the accuracy varied between 41 and 65.2 per cent. The test developed in this study yielded a large number of serological crossÁreactions.Conclusions:
The test can be employed to detect IgG antibodies within one arbovirus family; however, the hemagglutination test or other more specific techniques, such as the serum neutralization test in mice or the plaque-reduction neutralization test, are essential complementary methods for positive cases. (AU)
Biblioteca responsável:
BR275.1
Localização: PCIEC2014 / BR275.1
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