Norovirus genogroups I e II em amostras de água ambiental da cidade de Belém, no norte do Brasil/Norovirus genogroups I and II in environmental water samples from Belem city, Northern Brazil
Teixeira, Dielle Monteiro; Spada, Paula Katharine de Pontes; Morais, Lena Líllian Canto de Sá; Fumian, Túlio Machado; Lima, Ian Carlos Gomes de; Oliveira, Darleise de Souza; Bandeira, Renato da Silva; Gurjão, Tereza Cristina Monteiro; Sousa, Maísa Silva de; Mascarenhas, Joana D'Arc Pereira; Gabbay, Yvone Benchimol.
J Water Health
; 15(1): 163-174, 2017. ilus, tab
Artigo
em Inglês
| Instituto Evandro Chagas (SOPHIA) | ID: iec-16101
This study investigated the presence of norovirus (NoV) GI and GII in environmental samples from the northern region of Brazil. Water samples were collected monthly (November 2008/October 2010) from different sources and sewage and concentrated by the adsorption-elution method. The NoV investigation used molecular methods followed by sequencing reactions. The general positivity for NoV was 33.9% (57/168). Considering the results obtained only in the semi-nested RT-PCR (reverse transcription polymerase chain reaction) and only in the TaqMan (R) real-time PCR, the rates were 26.8% (45/168) and 27.4% (46/168), respectively, being for NoV GI 22.2% (10/45) and 19.6% (9/46); for GII 17.8% (8/45) and 15.2% (7/46); and for GI+GII 60% (27/45) and 65.2% (30/46), respectively. Different GI (GI. 1, GI. 4, GI. 7 and GI. 8) and GII (GII. 4, GII. 6, GII. 9, GII. 12 and GII. 14) genotypes were detected. These results demonstrated the NoV was disseminated in the waters of Belem city due to a lack of sanitation that allowed the discharge of contaminated effluents into these aquatic ecosystems
Biblioteca responsável:
BR275.1
Localização: PCIEC2017 / BR275.1
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