The ADA*2 allele of the adenosine deaminase gene (20q13.11) and recurrent spontaneous abortions: an age-dependent association

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Nunes, Daniela Prudente Teixeira; Spegiorin, Lígia Cosentino Junqueira Franco; Mattos, Cinara Cássia Brandão de; Oliani, Antonio Helio; Vaz-Oliani, Denise Cristina Mós; Mattos, Luiz Carlos de.

Clinics ; 66(11): 1929-1933, 2011. ilus, tab

Artigo em Inglês | LILACS | ID: lil-605874

OBJECTIVE:

Adenosine deaminase acts on adenosine and deoxyadenosine metabolism and modulates the immune response. The adenosine deaminase G22A polymorphism (20q.11.33) influences the level of adenosine deaminase enzyme expression, which seems to play a key role in maintaining pregnancy. The adenosine deaminase 2 phenotype has been associated with a protective effect against recurrent spontaneous abortions in European Caucasian women. The aim of this study was to investigate whether the G22A polymorphism of the adenosine deaminase gene is associated with recurrent spontaneous abortions in Brazilian women.

A total of 311 women were recruited to form two groups G1, with a history of recurrent spontaneous abortions (N = 129), and G2, without a history of abortions (N = 182). Genomic DNA was extracted from peripheral blood with a commercial kit and PCR-RFLP analysis was used to identify the G22A genetic polymorphism. Fisher's exact test and odds ratio values were used to compare the proportions of adenosine deaminase genotypes and alleles between women with and without a history of recurrent spontaneous abortion (p<0.05). The differences between mean values for categorical data were calculated using unpaired t tests. The Hardy-Weinberg equilibrium was assessed with a chi-square test.

Statistically significant differences were identified for the frequencies of adenosine deaminase genotypes and alleles between the G1 and G2 groups when adjusted for maternal age.

The results suggest that the adenosine deaminase *2 allele is associated with a low risk for recurrent spontaneous abortions, but this association is dependent on older age.

Adulto Feminino Humanos Gravidez Alelos Aborto Habitual/genética Adenosina Desaminase/genética Polimorfismo Genético/genética Fatores Etários Aborto Habitual/epidemiologia Aborto Espontâneo/epidemiologia Aborto Espontâneo/genética Brasil/epidemiologia Estudos de Casos e Controles Distribuição de Qui-Quadrado Genótipo

Biblioteca responsável: BR1.1