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Assembly of a Parts List of the Human Mitotic Cell Cycle Machinery.

Giotti, Bruno; Chen, Sz-Hau; Barnett, Mark W; Regan, Tim; Ly, Tony; Wiemann, Stefan; Hume, David A; Freeman, Tom C.
J Mol Cell Biol; 2018 Nov 17.
Artículo en Inglés | MEDLINE | ID: mdl-30452682
The set of proteins required for mitotic division remains poorly characterised. Here, an extensive series of correlation analyses of human and mouse transcriptomics data was performed to identify genes strongly and reproducibly associated with cells undergoing S/G2-M phases of the cell cycle. In so doing, a list of 701 cell cycle-associated genes were defined and whilst it was shown that many are only expressed during these phases, the expression of others is also driven by alternative promoters. Of this list, 496 genes have known cell cycle functions, whereas 205 were assigned as putative cell cycle genes, 53 of which are functionally uncharacterised. Among these, 27 were screened for subcellular localisation revealing many to be nuclear localised and at least four to be novel centrosomal proteins. Furthermore, 10 others inhibited cell proliferation upon siRNA knockdown. This study presents the first comprehensive list of human cell cycle proteins, identifying many new candidate proteins.