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Resultados  1-12 de 53

Insulated Isothermal Reverse Transcriptase PCR (iiRT-PCR) for Rapid and Sensitive Detection of Classical Swine Fever Virus.

Autor(es): Lung, O; Pasick, J; Fisher, M; Buchanan, C; Erickson, A; Ambagala, A
Fonte: Transbound Emerg Dis;63(5): e395-402, 2016 Oct.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 25644051
Resumo: Classical swine fever (CSF) is an OIE-listed disease that can have a severe impact on the swine industry. User-friendly, sensitive, rapid diagnostic tests that utilize low-cost field-deployable instruments for CSF diagnosis can be useful for disease surveillance and outbreak monitoring. In this study, we describe validation of a new probe-based insulated isothermal reverse transcriptase PCR (iiRT-PCR) assay for rapid detection of classical swine fever virus (CSFV) on a compact, user-friendl (mais)

Monitoramento de vírus respiratórios na região metropolitana de Belo Horizonte, 2011 a 2013/ Monitoreo de vírus respiratórios en la región metropolitana de Belo Horizonte, 2011-2013/ Monitoring respiratory virus infection in the metropolitan area of Belo Horizonte, Brazil, 2011-2013

Autor(es): Monteiro, Cristiane Campos; Dezanet, Lorenza Nogueira Campos; França, Elisabeth Barboza
Fonte: Epidemiol. serv. saúde;25(2): 233-242, abr.-jun. 2016. tab, graf
LILACS - Literatura Latino-Americana e do Caribe em Ciências da Saúde ID: 785217
Resumo: Resumo OBJETIVO: analisar a circulação dos vírus respiratórios em residentes na região metropolitana de Belo Horizonte, Brasil, hospitalizados em Belo Horizonte, de 2011 a 2013. MÉTODOS: estudo descritivo de 5.158 indivíduos com síndrome respiratória aguda grave; foram comparadas as características dos casos confirmados com casos descartados ou sem coleta de swab. RESULTADOS: metade dos vírus isolados foi da influenza A, especialmente os subtipos A(H1N1)pdm09 em pessoas de 20-59 (mais)

Evaluation of custom multiplex real - time RT - PCR in comparison to fast - track diagnostics respiratory 21 pathogens kit for detection of multiple respiratory viruses.

Autor(es): Malhotra, Bharti; Swamy, M Anjaneya; Reddy, P V Janardhan; Kumar, Neeraj; Tiwari, Jitendra Kumar
Fonte: Virol J;13: 91, 2016 Jun 06.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27267595
Resumo: BACKGROUND: Severe acute respiratory infections in children can be fatal, rapid identification of the causative agent and timely treatment can be life saving. Multiplex real time RT-PCR helps in simultaneous detection of multiple viruses saving cost, time and labour. Commercially available multiplex real time RT-PCR kits are very expensive. Therefore the aim of the present study was to develop a cost effective multiplex real time RT-PCR for the detection of 18 respiratory viruses and compar (mais)

Effects of previous episodes of influenza and vaccination in preventing laboratory-confirmed influenza in Navarre, Spain, 2013/14 season.

Autor(es): Castilla, Jesús; Navascués, Ana; Fernández-Alonso, Mirian; Reina, Gabriel; Albéniz, Esther; Pozo, Francisco; Álvarez, Nerea; Martínez-Baz, Iván; Guevara, Marcela; García-Cenoz, Manuel; Irisarri, Fátima; Casado, Itziar; Ezpeleta, Carmen
Fonte: Euro Surveill;20(22)2016 Jun 02.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27277013
Resumo: We estimated whether previous episodes of influenza and trivalent influenza vaccination prevented laboratory-confirmed influenza in Navarre, Spain, in season 2013/14. Patients with medically-attended influenza-like illness (MA-ILI) in hospitals (n = 645) and primary healthcare (n = 525) were included. We compared 589 influenza cases and 581 negative controls. MA-ILI related to a specific virus subtype in the previous five seasons was defined as a laboratory-confirmed influenza infection wit (mais)

Severe sensitivity loss in an influenza A molecular assay due to antigenic drift variants during the 2014/15 influenza season.

Autor(es): Overmeire, Yarah; Vanlaere, Elke; Hombrouck, Anneleen; De Beenhouwer, Hans; Simons, Guus; Brink, Antoinette; Van den Abeele, Anne-Marie; Verfaillie, Charlotte; Van Acker, Jos
Fonte: Diagn Microbiol Infect Dis;85(1): 42-6, 2016 May.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26964723
Resumo: The 2014-2015 influenza season in Belgium was dominated by the circulation of 2 influenza A(H3N2) subgroups: 3C.2a and 3C.3b. Analysis of 166 nasopharyngeal aspirates, collected in patients with respiratory illness at the start of the epidemic season, showed a decreased sensitivity for the detection of influenza A(H3N2)/3C.2a using a commercially available multiplex assay. Gene sequencing of the matrix protein showed a point mutation (C163T) leading to a mismatch with the assay probes.

Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco.

Autor(es): Fellahi, Siham; El Harrak, Mehdi; Kuhn, Jens H; Sebbar, Ghizlane; Bouaiti, El Arbi; Khataby, Khadija; Fihri, Ouafae Fassi; El Houadfi, Mohammed; Ennaji, My Mustapha
Fonte: BMC Res Notes;9: 231, 2016 Apr 22.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27106608
Resumo: BACKGROUND: A rapid, sensitive, and specific molecular method for the diagnosis of infectious bronchitis virus (IBV) infection is important in curbing infectious bronchitis outbreaks in Morocco and other countries. METHODS: In this study, an easy-to-perform SYBR green I real-time reverse transcriptase polymerase chain reaction (RT-PCR) targeting the nucleocapsid gene of IBV was developed and compared with conventional agarose gel-based RT-PCR for the detection of IBV infection. RESULTS: We (mais)

Performance of rapid SOFIA Influenza A+B test compared to Luminex x-TAG respiratory viral panel assay in the diagnosis of influenza A, B, and subtype H3.

Autor(es): Selove, W; Rao, L V
Fonte: J Investig Med;64(4): 905-7, 2016 Apr.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26911275
Resumo: Influenza is an acute respiratory illness caused by influenza A or B viruses that occur in outbreaks, mainly during the winter season. Rapid laboratory diagnosis of influenza can help guide the clinical management of suspected patients effectively. Clinical sensitivities and specificities of the rapid influenza diagnostic tests have varied considerably in the literature. Most of these studies are evaluated using previously frozen or stored specimens that had previously tested positive. This (mais)

A Rapid and Automated Device for Purifying Nucleic Acids.

Autor(es): Uehara, Masayuki; Hanamura, Masato; Yamada, Kiyohito; Yamaguchi, Akemi; Murayama, Toshiro; Saito, Yuji; Idegami, Kotaro; Honda, Takayuki
Fonte: Anal Sci;32(3): 371-4, 2016.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26960621
Resumo: We have developed a rapid, automated nucleic acid purification device in a single cartridge containing silica-coated magnetic beads. We succeeded in extracting the matrix protein gene of influenza A virus from pharyngeal swab samples within 3 min. The device will be widely applicable to detect a specific gene from the various samples for clinical diagnosis and genetic research.
Resultados  1-12 de 53