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Resultados  1-12 de 45

Monitoramento de vírus respiratórios na região metropolitana de Belo Horizonte, 2011 a 2013/ Monitoreo de vírus respiratórios en la región metropolitana de Belo Horizonte, 2011-2013/ Monitoring respiratory virus infection in the metropolitan area of Belo Horizonte, Brazil, 2011-2013

Autor(es): Monteiro, Cristiane Campos; Dezanet, Lorenza Nogueira Campos; França, Elisabeth Barboza
Fonte: Epidemiol. serv. saúde;25(2): 233-242, abr.-jun. 2016. tab, graf
LILACS - Literatura Latino-Americana e do Caribe em Ciências da Saúde ID: 785217
Resumo: OBJETIVO: analisar a circulação dos vírus respiratórios em residentes na região metropolitana de Belo Horizonte, Brasil, hospitalizados em Belo Horizonte, de 2011 a 2013. MÉTODOS: estudo descritivo de 5.158 indivíduos com síndrome respiratória aguda grave; foram comparadas as características dos casos confirmados com casos descartados ou sem coleta de swab. RESULTADOS: metade dos vírus isolados foi da influenza A, especialmente os subtipos A(H1N1)pdm09 em pessoas de 20-59 anos e (mais)

Evaluation of custom multiplex real - time RT - PCR in comparison to fast - track diagnostics respiratory 21 pathogens kit for detection of multiple respiratory viruses.

Autor(es): Malhotra, Bharti; Swamy, M Anjaneya; Reddy, P V Janardhan; Kumar, Neeraj; Tiwari, Jitendra Kumar
Fonte: Virol J;13: 91, 2016 Jun 06.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27267595
Resumo: BACKGROUND: Severe acute respiratory infections in children can be fatal, rapid identification of the causative agent and timely treatment can be life saving. Multiplex real time RT-PCR helps in simultaneous detection of multiple viruses saving cost, time and labour. Commercially available multiplex real time RT-PCR kits are very expensive. Therefore the aim of the present study was to develop a cost effective multiplex real time RT-PCR for the detection of 18 respiratory viruses and compar (mais)

Effects of previous episodes of influenza and vaccination in preventing laboratory-confirmed influenza in Navarre, Spain, 2013/14 season.

Autor(es): Castilla, Jesús; Navascués, Ana; Fernández-Alonso, Mirian; Reina, Gabriel; Albéniz, Esther; Pozo, Francisco; Álvarez, Nerea; Martínez-Baz, Iván; Guevara, Marcela; García-Cenoz, Manuel; Irisarri, Fátima; Casado, Itziar; Ezpeleta, Carmen
Fonte: Euro Surveill;20(22)2016 Jun 02.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27277013
Resumo: We estimated whether previous episodes of influenza and trivalent influenza vaccination prevented laboratory-confirmed influenza in Navarre, Spain, in season 2013/14. Patients with medically-attended influenza-like illness (MA-ILI) in hospitals (n = 645) and primary healthcare (n = 525) were included. We compared 589 influenza cases and 581 negative controls. MA-ILI related to a specific virus subtype in the previous five seasons was defined as a laboratory-confirmed influenza infection wit (mais)

Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco.

Autor(es): Fellahi, Siham; El Harrak, Mehdi; Kuhn, Jens H; Sebbar, Ghizlane; Bouaiti, El Arbi; Khataby, Khadija; Fihri, Ouafae Fassi; El Houadfi, Mohammed; Ennaji, My Mustapha
Fonte: BMC Res Notes;9: 231, 2016 Apr 22.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27106608
Resumo: BACKGROUND: A rapid, sensitive, and specific molecular method for the diagnosis of infectious bronchitis virus (IBV) infection is important in curbing infectious bronchitis outbreaks in Morocco and other countries. METHODS: In this study, an easy-to-perform SYBR green I real-time reverse transcriptase polymerase chain reaction (RT-PCR) targeting the nucleocapsid gene of IBV was developed and compared with conventional agarose gel-based RT-PCR for the detection of IBV infection. RESULTS: We (mais)

Interim estimates of 2015/16 vaccine effectiveness against influenza A(H1N1)pdm09, Canada, February 2016.

Autor(es): Chambers, Catharine; Skowronski, Danuta M; Sabaiduc, Suzana; Winter, Anne Luise; Dickinson, James A; De Serres, Gaston; Gubbay, Jonathan B; Drews, Steven J; Martineau, Christine; Eshaghi, Alireza; Krajden, Mel; Bastien, Nathalie; Li, Yan
Fonte: Euro Surveill;21(11): 30168, 2016.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27020673
Resumo: Using a test-negative design, the Canadian Sentinel Practitioner Surveillance Network (SPSN) assessed interim 2015/16 vaccine effectiveness (VE) against influenza A(H1N1)pdm09 viruses. Adjusted VE showed significant protection of 64% (95% confidence interval (CI): 44-77%) overall and 56% (95%CI: 26-73%) for adults between 20 and 64 years-old against medically attended, laboratory-confirmed A(H1N1)pdm09 illness. Among the 67 A(H1N1)pdm09-positive specimens that were successfully sequenced, 6 (mais)

Emergence of influenza A (H1N1)pdm09 genogroup 6B and drug resistant virus, India, January to May 2015.

Autor(es): Parida, Manmohan; Dash, Paban Kumar; Kumar, Jyoti S; Joshi, Gaurav; Tandel, Kundan; Sharma, Shashi; Srivastava, Ambuj; Agarwal, Ankita; Saha, Amrita; Saraswat, Shweta; Karothia, Divyanshi; Malviya, Vatsala
Fonte: Euro Surveill;21(5): 6-11, 2016.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26876980
Resumo: To investigate the aetiology of the 2015 A(H1N1)pdm09 influenza outbreak in India, 1,083 nasopharyngeal swabs from suspect patients were screened for influenza A(H1N1)pdm09 in the state of Madhya Pradesh. Of 412 positive specimens, six were further characterised by phylogenetic analysis of haemagglutinin (HA) sequences revealing that they belonged to genogroup 6B. A new mutation (E164G) was observed in HA2 of two sequences. Neuraminidase genes in two of 12 isolates from fatal cases on prior oseltamivir treatment harboured the H275Y mutation.

Dual-role FilmArray® diagnostics for high-impact viral diseases.

Autor(es): Dorsch, M; Meehan, B; Michalski, W P; Heine, H G; Foord, A J; Carlile, G; Wang, J; McCullough, S; Zuelke, K
Fonte: Aust Vet J;94(3): 64-6, 2016 Mar.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26914951
Resumo: In this study, we explored the potential utility of the human-focused FilmArray® Respiratory Panel for the diagnosis of a broad range of influenza viruses of veterinary concern as compared with the standard portfolio of recommended TaqMan®-based diagnostic tests. In addition, we discuss some potential operational advantages associated with the use of such integrated sample extraction, amplification and analysis devices in the context of a future long-term, dual-role strategy for the detection of emergency diseases of both human and veterinary concern.

Ring test evaluation of the detection of influenza A virus in swine oral fluids by real-time reverse-transcription polymerase chain reaction and virus isolation.

Autor(es): Goodell, Christa K; Zhang, Jianqiang; Strait, Erin; Harmon, Karen; Patnayak, Devi; Otterson, Tracy; Culhane, Marie; Christopher-Hennings, Jane; Clement, Travis; Leslie-Steen, Pamela; Hesse, Richard; Anderson, Joe; Skarbek, Kevin; Vincent, Amy; Kitikoon, Pravina; Swenson, Sabrina; Jenkins-Moore, Melinda; McGill, Jodi; Rauh, Rolf; Nelson, William; O'Connell, Catherine; Shah, Rohan; Wang, Chong; Main, Rodger; Zimmerman, Jeffrey J
Fonte: Can J Vet Res;80(1): 12-20, 2016 Jan.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26733728
Resumo: The probability of detecting influenza A virus (IAV) in oral fluid (OF) specimens was calculated for each of 13 assays based on real-time reverse-transcription polymerase chain reaction (rRT-PCR) and 7 assays based on virus isolation (VI). The OF specimens were inoculated with H1N1 or H3N2 IAV and serially diluted 10-fold (10(-1) to 10(-8)). Eight participating laboratories received 180 randomized OF samples (10 replicates × 8 dilutions × 2 IAV subtypes plus 20 IAV-negative samples) and p (mais)

Multiplex RT-PCR detection of H3N2 influenza A virus in dogs.

Autor(es): Lee, EunJung; Kim, Eun-Ju; Kim, Bo-Hye; Song, Jae-Young; Cho, In-Soo; Shin, Yeun-Kyung
Fonte: Mol Cell Probes;30(1): 56-60, 2016 Feb.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26738688
Resumo: A multiplex RT-PCR (mRT-PCR) assay to detect H3N2 CIV genomic segments was developed as a rapid and cost-effective method. Its performance was evaluated with forty-six influenza A viruses from different hosts using three primer sets which amplify four segments of H3N2 CIV simultaneously. The mRT-PCR has been successful in detecting the viral segments, indicating that it can improve the speed of diagnosis for H3N2 CIV and its reassortants.
Resultados  1-12 de 45