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Resultados  1-12 de 49

Insulated Isothermal Reverse Transcriptase PCR (iiRT-PCR) for Rapid and Sensitive Detection of Classical Swine Fever Virus.

Autor(es): Lung, O; Pasick, J; Fisher, M; Buchanan, C; Erickson, A; Ambagala, A
Fonte: Transbound Emerg Dis;63(5): e395-402, 2016 Oct.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 25644051
Resumo: Classical swine fever (CSF) is an OIE-listed disease that can have a severe impact on the swine industry. User-friendly, sensitive, rapid diagnostic tests that utilize low-cost field-deployable instruments for CSF diagnosis can be useful for disease surveillance and outbreak monitoring. In this study, we describe validation of a new probe-based insulated isothermal reverse transcriptase PCR (iiRT-PCR) assay for rapid detection of classical swine fever virus (CSFV) on a compact, user-friendl (mais)

Evaluation of custom multiplex real - time RT - PCR in comparison to fast - track diagnostics respiratory 21 pathogens kit for detection of multiple respiratory viruses.

Autor(es): Malhotra, Bharti; Swamy, M Anjaneya; Reddy, P V Janardhan; Kumar, Neeraj; Tiwari, Jitendra Kumar
Fonte: Virol J;13: 91, 2016 Jun 06.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27267595
Resumo: BACKGROUND: Severe acute respiratory infections in children can be fatal, rapid identification of the causative agent and timely treatment can be life saving. Multiplex real time RT-PCR helps in simultaneous detection of multiple viruses saving cost, time and labour. Commercially available multiplex real time RT-PCR kits are very expensive. Therefore the aim of the present study was to develop a cost effective multiplex real time RT-PCR for the detection of 18 respiratory viruses and compar (mais)

Effects of previous episodes of influenza and vaccination in preventing laboratory-confirmed influenza in Navarre, Spain, 2013/14 season.

Autor(es): Castilla, Jesús; Navascués, Ana; Fernández-Alonso, Mirian; Reina, Gabriel; Albéniz, Esther; Pozo, Francisco; Álvarez, Nerea; Martínez-Baz, Iván; Guevara, Marcela; García-Cenoz, Manuel; Irisarri, Fátima; Casado, Itziar; Ezpeleta, Carmen
Fonte: Euro Surveill;20(22)2016 Jun 02.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27277013
Resumo: We estimated whether previous episodes of influenza and trivalent influenza vaccination prevented laboratory-confirmed influenza in Navarre, Spain, in season 2013/14. Patients with medically-attended influenza-like illness (MA-ILI) in hospitals (n = 645) and primary healthcare (n = 525) were included. We compared 589 influenza cases and 581 negative controls. MA-ILI related to a specific virus subtype in the previous five seasons was defined as a laboratory-confirmed influenza infection wit (mais)

Severe sensitivity loss in an influenza A molecular assay due to antigenic drift variants during the 2014/15 influenza season.

Autor(es): Overmeire, Yarah; Vanlaere, Elke; Hombrouck, Anneleen; De Beenhouwer, Hans; Simons, Guus; Brink, Antoinette; Van den Abeele, Anne-Marie; Verfaillie, Charlotte; Van Acker, Jos
Fonte: Diagn Microbiol Infect Dis;85(1): 42-6, 2016 May.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26964723
Resumo: The 2014-2015 influenza season in Belgium was dominated by the circulation of 2 influenza A(H3N2) subgroups: 3C.2a and 3C.3b. Analysis of 166 nasopharyngeal aspirates, collected in patients with respiratory illness at the start of the epidemic season, showed a decreased sensitivity for the detection of influenza A(H3N2)/3C.2a using a commercially available multiplex assay. Gene sequencing of the matrix protein showed a point mutation (C163T) leading to a mismatch with the assay probes.

Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco.

Autor(es): Fellahi, Siham; El Harrak, Mehdi; Kuhn, Jens H; Sebbar, Ghizlane; Bouaiti, El Arbi; Khataby, Khadija; Fihri, Ouafae Fassi; El Houadfi, Mohammed; Ennaji, My Mustapha
Fonte: BMC Res Notes;9: 231, 2016 Apr 22.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27106608
Resumo: BACKGROUND: A rapid, sensitive, and specific molecular method for the diagnosis of infectious bronchitis virus (IBV) infection is important in curbing infectious bronchitis outbreaks in Morocco and other countries. METHODS: In this study, an easy-to-perform SYBR green I real-time reverse transcriptase polymerase chain reaction (RT-PCR) targeting the nucleocapsid gene of IBV was developed and compared with conventional agarose gel-based RT-PCR for the detection of IBV infection. RESULTS: We (mais)

Performance of rapid SOFIA Influenza A+B test compared to Luminex x-TAG respiratory viral panel assay in the diagnosis of influenza A, B, and subtype H3.

Autor(es): Selove, W; Rao, L V
Fonte: J Investig Med;64(4): 905-7, 2016 Apr.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26911275
Resumo: Influenza is an acute respiratory illness caused by influenza A or B viruses that occur in outbreaks, mainly during the winter season. Rapid laboratory diagnosis of influenza can help guide the clinical management of suspected patients effectively. Clinical sensitivities and specificities of the rapid influenza diagnostic tests have varied considerably in the literature. Most of these studies are evaluated using previously frozen or stored specimens that had previously tested positive. This (mais)

A Rapid and Automated Device for Purifying Nucleic Acids.

Autor(es): Uehara, Masayuki; Hanamura, Masato; Yamada, Kiyohito; Yamaguchi, Akemi; Murayama, Toshiro; Saito, Yuji; Idegami, Kotaro; Honda, Takayuki
Fonte: Anal Sci;32(3): 371-4, 2016.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26960621
Resumo: We have developed a rapid, automated nucleic acid purification device in a single cartridge containing silica-coated magnetic beads. We succeeded in extracting the matrix protein gene of influenza A virus from pharyngeal swab samples within 3 min. The device will be widely applicable to detect a specific gene from the various samples for clinical diagnosis and genetic research.

Interim estimates of 2015/16 vaccine effectiveness against influenza A(H1N1)pdm09, Canada, February 2016.

Autor(es): Chambers, Catharine; Skowronski, Danuta M; Sabaiduc, Suzana; Winter, Anne Luise; Dickinson, James A; De Serres, Gaston; Gubbay, Jonathan B; Drews, Steven J; Martineau, Christine; Eshaghi, Alireza; Krajden, Mel; Bastien, Nathalie; Li, Yan
Fonte: Euro Surveill;21(11): 30168, 2016.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27020673
Resumo: Using a test-negative design, the Canadian Sentinel Practitioner Surveillance Network (SPSN) assessed interim 2015/16 vaccine effectiveness (VE) against influenza A(H1N1)pdm09 viruses. Adjusted VE showed significant protection of 64% (95% confidence interval (CI): 44-77%) overall and 56% (95%CI: 26-73%) for adults between 20 and 64 years-old against medically attended, laboratory-confirmed A(H1N1)pdm09 illness. Among the 67 A(H1N1)pdm09-positive specimens that were successfully sequenced, 6 (mais)

Emergence of influenza A (H1N1)pdm09 genogroup 6B and drug resistant virus, India, January to May 2015.

Autor(es): Parida, Manmohan; Dash, Paban Kumar; Kumar, Jyoti S; Joshi, Gaurav; Tandel, Kundan; Sharma, Shashi; Srivastava, Ambuj; Agarwal, Ankita; Saha, Amrita; Saraswat, Shweta; Karothia, Divyanshi; Malviya, Vatsala
Fonte: Euro Surveill;21(5): 6-11, 2016.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26876980
Resumo: To investigate the aetiology of the 2015 A(H1N1)pdm09 influenza outbreak in India, 1,083 nasopharyngeal swabs from suspect patients were screened for influenza A(H1N1)pdm09 in the state of Madhya Pradesh. Of 412 positive specimens, six were further characterised by phylogenetic analysis of haemagglutinin (HA) sequences revealing that they belonged to genogroup 6B. A new mutation (E164G) was observed in HA2 of two sequences. Neuraminidase genes in two of 12 isolates from fatal cases on prior oseltamivir treatment harboured the H275Y mutation.

Dual-role FilmArray® diagnostics for high-impact viral diseases.

Autor(es): Dorsch, M; Meehan, B; Michalski, W P; Heine, H G; Foord, A J; Carlile, G; Wang, J; McCullough, S; Zuelke, K
Fonte: Aust Vet J;94(3): 64-6, 2016 Mar.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26914951
Resumo: In this study, we explored the potential utility of the human-focused FilmArray® Respiratory Panel for the diagnosis of a broad range of influenza viruses of veterinary concern as compared with the standard portfolio of recommended TaqMan®-based diagnostic tests. In addition, we discuss some potential operational advantages associated with the use of such integrated sample extraction, amplification and analysis devices in the context of a future long-term, dual-role strategy for the detection of emergency diseases of both human and veterinary concern.
Resultados  1-12 de 49