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Resultados  1-12 de 40

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR.

Autor(es): Goodman, Laura B; Anderson, Renee R; Slater, Marcia; Ortenberg, Elen; Renshaw, Randall W; Chilson, Brittany D; Laverack, Melissa A; Beeby, John S; Dubovi, Edward J; Glaser, Amy L
Fonte: J Vis Exp;(117)2016 Nov 28.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27929456
Resumo: Nanoliter scale real-time PCR uses spatial multiplexing to allow multiple assays to be run in parallel on a single plate without the typical drawbacks of combining reactions together. We designed and evaluated a panel based on this principle to rapidly identify the presence of common disease agents in dogs and horses with acute respiratory illness. This manuscript describes a nanoscale diagnostic PCR workflow for sample preparation, amplification, and analysis of target pathogen sequences, (mais)

Forecasting Influenza Outbreaks in Boroughs and Neighborhoods of New York City.

Autor(es): Yang, Wan; Olson, Donald R; Shaman, Jeffrey
Fonte: PLoS Comput Biol;12(11): e1005201, 2016 Nov.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27855155
Resumo: The ideal spatial scale, or granularity, at which infectious disease incidence should be monitored and forecast has been little explored. By identifying the optimal granularity for a given disease and host population, and matching surveillance and prediction efforts to this scale, response to emergent and recurrent outbreaks can be improved. Here we explore how granularity and representation of spatial structure affect influenza forecast accuracy within New York City. We develop network mod (mais)

Detection of porcine reproductive and respiratory syndrome virus (PRRSV) and influenza A virus (IAV) in oral fluid of pigs.

Autor(es): Biernacka, Kinga; Karbowiak, Pawel; Wróbel, Pawel; Chareza, Tomasz; Czopowicz, Michal; Balka, Gyula; Goodell, Christa; Rauh, Rolf; Stadejek, Tomasz
Fonte: Res Vet Sci;109: 74-80, 2016 Dec.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27892877
Resumo: Recently oral fluid has become a novel sample type for pathogen nucleic acid and antibody detection, as it is easy to obtain with non-invasive procedures. The objective of the study was to analyze porcine reproductive and respiratory syndrome virus (PRRSV) and influenza A virus (IAV) circulation in growing pigs from three Polish production farms, using Real Time PCR and ELISA testing of oral fluid and serum. Oral fluids were collected every 2weeks, in the same 3-4 pens of pigs aged between (mais)

Integrated microfluidic device using a single universal aptamer to detect multiple types of influenza viruses.

Autor(es): Wang, Chih-Hung; Chang, Chih-Peng; Lee, Gwo-Bin
Fonte: Biosens Bioelectron;86: 247-254, 2016 Dec 15.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27376195
Resumo: DNA aptamers that can bind specific molecular targets have great potential as probes for microbial diagnostic applications. However, aptamers may change their conformation under different operating conditions, thus affecting their affinity and specificity towards the target molecules. In this study, a new integrated microfluidic system was developed that exploited the predictable change in conformation of a single universal influenza aptamer exposed to differing ion concentrations in order (mais)

Effects of previous episodes of influenza and vaccination in preventing laboratory-confirmed influenza in Navarre, Spain, 2013/14 season.

Autor(es): Castilla, Jesús; Navascués, Ana; Fernández-Alonso, Mirian; Reina, Gabriel; Albéniz, Esther; Pozo, Francisco; Álvarez, Nerea; Martínez-Baz, Iván; Guevara, Marcela; García-Cenoz, Manuel; Irisarri, Fátima; Casado, Itziar; Ezpeleta, Carmen
Fonte: Euro Surveill;20(22)2016 Jun 02.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27277013
Resumo: We estimated whether previous episodes of influenza and trivalent influenza vaccination prevented laboratory-confirmed influenza in Navarre, Spain, in season 2013/14. Patients with medically-attended influenza-like illness (MA-ILI) in hospitals (n = 645) and primary healthcare (n = 525) were included. We compared 589 influenza cases and 581 negative controls. MA-ILI related to a specific virus subtype in the previous five seasons was defined as a laboratory-confirmed influenza infection wit (mais)

Variability in the diagnostic performance of a bedside rapid diagnostic influenza test over four epidemic seasons in a pediatric emergency department.

Autor(es): Avril, E; Lacroix, S; Vrignaud, B; Moreau-Klein, A; Coste-Burel, M; Launay, E; Gras-Le Guen, C
Fonte: Diagn Microbiol Infect Dis;85(3): 334-337, 2016 Jul.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27139081
Resumo: We wanted to determine the diagnostic performance of a rapid influenza diagnostic test (RIDT) used bedside in a pediatric emergency department (PED). This was a prospective study over four consecutive winters (2009-2013), comparing the results of a RIDT (QuickVue®) with RT-PCR in children admitted to a PED. Among the 764 children included, we did not observe any significant differences in the diagnostic performance of RIDT except during the H1N1 pandemic. The overall sensitivity of the tes (mais)

Trypsin pre-treatment corrects SRID over-estimation of immunologically active, pre-fusion HA caused by mixed immunoprecipitin rings.

Autor(es): Wen, Yingxia; Palladino, Giuseppe; Xie, Yuhong; Ferrari, Annette; Ma, Xiuwen; Han, Liqun; Dormitzer, Philip R; Settembre, Ethan C
Fonte: Vaccine;34(29): 3388-95, 2016 Jun 17.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27154389
Resumo: Influenza vaccines are the primary intervention to prevent the substantial health burden of seasonal and pandemic influenza. Subunit and split influenza vaccines are formulated, released for clinical use, and tested for stability based on their content of immunologically active (capable of eliciting functional antibodies) hemagglutinin (HA). Single-radial immunodiffusion (SRID), the standard in vitro potency assay in the field, is believed to specifically detect immunologically active HA. W (mais)

The composition of immune cells serves as a predictor of adaptive immunity in a cohort of 50- to 74-year-old adults.

Autor(es): Kennedy, Richard B; Simon, Whitney L; Gibson, Michael J; Goergen, Krista M; Grill, Diane E; Oberg, Ann L; Poland, Gregory A
Fonte: Immunology;148(3): 266-75, 2016 07.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27188667
Resumo: Influenza causes significant morbidity and mortality annually. Although vaccination offers a considerable amount of protection, it is far from perfect, especially in aging populations. This is due to age-related defects in immune function, a process called immunosenescence. To date, there are no assays or methods to predict or explain variations in an individual's level of response to influenza vaccination. In this study, we measured levels of several immune cell subsets at baseline (Day 0) (mais)

Comparison of hemagglutination inhibition and microbead array assays for the measurement of influenza antibody levels in HIV-infected adults.

Autor(es): Thorne, Anona; Chowdhury, Fahad; Singer, Joel; Keynan, Yoav; Fowke, Keith R; Cooper, Curtis
Fonte: Vaccine;34(31): 3584-91, 2016 Jun 30.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27211040
Resumo: INTRODUCTION: Determining vaccine responsiveness is challenging in immune compromised individuals. The microbead array (MBA) assay is a rapid, inexpensive test for evaluating influenza vaccine immunogenicity. MBA performance was compared to hemagglutination inhibition assay (HAI) utilizing HIV seropositive vaccine recipient specimens. METHODS: CTN 253 evaluated standard dose, single antigen, inactivated split adjuvanted (AS03(A)) H1N1 influenza vaccine (Arepanrix) vs standard dose plus boos (mais)

Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco.

Autor(es): Fellahi, Siham; El Harrak, Mehdi; Kuhn, Jens H; Sebbar, Ghizlane; Bouaiti, El Arbi; Khataby, Khadija; Fihri, Ouafae Fassi; El Houadfi, Mohammed; Ennaji, My Mustapha
Fonte: BMC Res Notes;9: 231, 2016 Apr 22.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27106608
Resumo: BACKGROUND: A rapid, sensitive, and specific molecular method for the diagnosis of infectious bronchitis virus (IBV) infection is important in curbing infectious bronchitis outbreaks in Morocco and other countries. METHODS: In this study, an easy-to-perform SYBR green I real-time reverse transcriptase polymerase chain reaction (RT-PCR) targeting the nucleocapsid gene of IBV was developed and compared with conventional agarose gel-based RT-PCR for the detection of IBV infection. RESULTS: We (mais)

Concordance of interim and final estimates of influenza vaccine effectiveness: a systematic review.

Autor(es): Leung, Vivian K; Cowling, Benjamin J; Feng, Shuo; Sullivan, Sheena G
Fonte: Euro Surveill;21(16)2016 Apr 21.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27124573
Resumo: The World Health Organization's Global Influenza Surveillance and Response System meets twice a year to generate a recommendation for the composition of the seasonal influenza vaccine. Interim vaccine effectiveness (VE) estimates provide a preliminary indication of influenza vaccine performance during the season and may be useful for decision making. We reviewed 17 pairs of studies reporting 33 pairs of interim and final estimates using the test-negative design to evaluate whether interim e (mais)
Resultados  1-12 de 40