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Resultados  1-12 de 53

Contribution of a rapid influenza diagnostic test to manage hospitalized patients with suspected influenza.

Autor(es): Busson, Laurent; Mahadeb, Bhavna; De Foor, Marc; Vandenberg, Olivier; Hallin, Marie
Fonte: Diagn Microbiol Infect Dis;87(3): 238-242, 2017 Mar.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27939284
Resumo: AIM: To evaluate the performances of the Alere i influenza A&B test and to appraise its contribution to patient management. METHODS: In total, 267 samples were tested. Influenza A and B PCR was performed as the reference. For each positive result, the supervising physician was contacted to collect data regarding patient management. FINDINGS: The overall sensitivity and specificity of the Alere i were 91.4% and 97.6% for influenza A and 54.5% and 98.8% for influenza B, respectively. More spe (mais)

Detection of porcine reproductive and respiratory syndrome virus (PRRSV) and influenza A virus (IAV) in oral fluid of pigs.

Autor(es): Biernacka, Kinga; Karbowiak, Pawel; Wróbel, Pawel; Chareza, Tomasz; Czopowicz, Michal; Balka, Gyula; Goodell, Christa; Rauh, Rolf; Stadejek, Tomasz
Fonte: Res Vet Sci;109: 74-80, 2016 Dec.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27892877
Resumo: Recently oral fluid has become a novel sample type for pathogen nucleic acid and antibody detection, as it is easy to obtain with non-invasive procedures. The objective of the study was to analyze porcine reproductive and respiratory syndrome virus (PRRSV) and influenza A virus (IAV) circulation in growing pigs from three Polish production farms, using Real Time PCR and ELISA testing of oral fluid and serum. Oral fluids were collected every 2weeks, in the same 3-4 pens of pigs aged between (mais)

Evaluation of the rapid influenza detection tests GOLD SIGN FLU and Quick Navi-Flu for the detection of influenza A and B virus antigens in adults during the influenza season.

Autor(es): Akaishi, Yu; Matsumoto, Tetsuya; Harada, Yoshimi; Hirayama, Yoji
Fonte: Int J Infect Dis;52: 55-58, 2016 Nov.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27720945
Resumo: As the characteristics and accuracy of rapid influenza detection tests (RIDTs) vary, the development of a high-performance RIDT has been eagerly anticipated. In this study, the new RIDT GOLD SIGN FLU and the existing RIDT Quick Navi-Flu were evaluated in terms of detecting the antigens of influenza viruses A and B in Japanese adults with influenza-like symptoms. The study was performed from December 2013 to March 2014. Among the 123 patients from whom nasopharyngeal swab specimens were coll (mais)

Sensitive detection of influenza viruses with Europium nanoparticles on an epoxy silica sol-gel functionalized polycarbonate-polydimethylsiloxane hybrid microchip.

Autor(es): Liu, Jikun; Zhao, Jiangqin; Petrochenko, Peter; Zheng, Jiwen; Hewlett, Indira
Fonte: Biosens Bioelectron;86: 150-5, 2016 Dec 15.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27362253
Resumo: In an effort to develop new tools for diagnosing influenza in resource-limited settings, we fabricated a polycarbonate (PC)-polydimethylsiloxane (PDMS) hybrid microchip using a simple epoxy silica sol-gel coating/bonding method and employed it in sensitive detection of influenza virus with Europium nanoparticles (EuNPs). The incorporation of sol-gel material in device fabrication provided functionalized channel surfaces ready for covalent immobilization of primary antibodies and a strong bo (mais)

Integrated microfluidic device using a single universal aptamer to detect multiple types of influenza viruses.

Autor(es): Wang, Chih-Hung; Chang, Chih-Peng; Lee, Gwo-Bin
Fonte: Biosens Bioelectron;86: 247-54, 2016 Dec 15.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27376195
Resumo: DNA aptamers that can bind specific molecular targets have great potential as probes for microbial diagnostic applications. However, aptamers may change their conformation under different operating conditions, thus affecting their affinity and specificity towards the target molecules. In this study, a new integrated microfluidic system was developed that exploited the predictable change in conformation of a single universal influenza aptamer exposed to differing ion concentrations in order (mais)

Insulated Isothermal Reverse Transcriptase PCR (iiRT-PCR) for Rapid and Sensitive Detection of Classical Swine Fever Virus.

Autor(es): Lung, O; Pasick, J; Fisher, M; Buchanan, C; Erickson, A; Ambagala, A
Fonte: Transbound Emerg Dis;63(5): e395-402, 2016 Oct.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 25644051
Resumo: Classical swine fever (CSF) is an OIE-listed disease that can have a severe impact on the swine industry. User-friendly, sensitive, rapid diagnostic tests that utilize low-cost field-deployable instruments for CSF diagnosis can be useful for disease surveillance and outbreak monitoring. In this study, we describe validation of a new probe-based insulated isothermal reverse transcriptase PCR (iiRT-PCR) assay for rapid detection of classical swine fever virus (CSFV) on a compact, user-friendl (mais)

Evaluation of a smartphone-based rapid fluorescent diagnostic system for H9N2 virus in specific-pathogen-free chickens.

Autor(es): Yeo, Seon-Ju; Cuc, Bui Thi; Sung, Haan Woo; Park, Hyun
Fonte: Arch Virol;161(8): 2249-56, 2016 Aug.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27287435
Resumo: Repeated interspecies transmission of H9N2 virus from poultry to humans and human infections transmitted via aerosols highlight the need for a highly sensitive, rapid diagnostic system for the detection of this virus. However, no such test exhibiting high performance has been developed. In this study, the performance of a smartphone-based rapid fluorescent diagnostic system (SRFDS) was optimized for the diagnosis of an H9N2-virus-infected animal. To suppress the nonspecific reactivity of th (mais)

Evaluation of the Alere i Influenza A&B assay for rapid identification of influenza A and influenza B viruses.

Autor(es): Chiarella, Flavia Cecilia; Culebras, Esther; Fuentes-Ferrer, Manuel Enrique; Picazo, Juan José
Fonte: J Med Microbiol;65(6): 456-61, 2016 Jun.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 26967368
Resumo: The Alere i Influenza A&B assay is a novel isothermal nucleic acid amplification assay capable of detecting and differentiating influenza A and B viruses in approximately 15 min with minimal hands-on time. This study was conducted in order to assess the performance of the Alere i Influenza A&B assay compared to molecular techniques, considered to be gold standard methods, to evaluate the results. A total of 119 nasopharyngeal swabs collected from inpatients with influenza-like illness were (mais)

Variability in the diagnostic performance of a bedside rapid diagnostic influenza test over four epidemic seasons in a pediatric emergency department.

Autor(es): Avril, E; Lacroix, S; Vrignaud, B; Moreau-Klein, A; Coste-Burel, M; Launay, E; Gras-Le Guen, C
Fonte: Diagn Microbiol Infect Dis;85(3): 334-7, 2016 Jul.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27139081
Resumo: We wanted to determine the diagnostic performance of a rapid influenza diagnostic test (RIDT) used bedside in a pediatric emergency department (PED). This was a prospective study over four consecutive winters (2009-2013), comparing the results of a RIDT (QuickVue®) with RT-PCR in children admitted to a PED. Among the 764 children included, we did not observe any significant differences in the diagnostic performance of RIDT except during the H1N1 pandemic. The overall sensitivity of the tes (mais)

Effects of previous episodes of influenza and vaccination in preventing laboratory-confirmed influenza in Navarre, Spain, 2013/14 season.

Autor(es): Castilla, Jesús; Navascués, Ana; Fernández-Alonso, Mirian; Reina, Gabriel; Albéniz, Esther; Pozo, Francisco; Álvarez, Nerea; Martínez-Baz, Iván; Guevara, Marcela; García-Cenoz, Manuel; Irisarri, Fátima; Casado, Itziar; Ezpeleta, Carmen
Fonte: Euro Surveill;20(22)2016 Jun 02.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27277013
Resumo: We estimated whether previous episodes of influenza and trivalent influenza vaccination prevented laboratory-confirmed influenza in Navarre, Spain, in season 2013/14. Patients with medically-attended influenza-like illness (MA-ILI) in hospitals (n = 645) and primary healthcare (n = 525) were included. We compared 589 influenza cases and 581 negative controls. MA-ILI related to a specific virus subtype in the previous five seasons was defined as a laboratory-confirmed influenza infection wit (mais)

Comparison of two new generation influenza rapid diagnostic tests with instrument-based digital readout systems for influenza virus detection.

Autor(es): Ryu, S-W; Lee, J-H; Kim, J; Jang, M-A; Nam, J-H; Byoun, M-S; Lim, C S
Fonte: Br J Biomed Sci;73(3): 115-120, 2016 Jul.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27327199
Resumo: INTRODUCTION: Influenza rapid diagnostic tests (RDTs) have been developed to supply scientists with more sensitive and specific techniques. Newly developed digital reader-based techniques require test evaluations before their clinical application. METHODS: Two types of digital influenza RDTs using a digital readout system and one conventional RDT were compared using 314 nasopharyngeal swabs of influenza. The swabs originated from symptomatic individuals suspected of influenza infection, and (mais)
Resultados  1-12 de 53