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Resultados  1-12 de 64

Influenza A Viruses of Swine (IAV-S) in Vietnam from 2010 to 2015: Multiple Introductions of A(H1N1)pdm09 Viruses into the Pig Population and Diversifying Genetic Constellations of Enzootic IAV-S.

Autor(es): Takemae, Nobuhiro; Harada, Michiyo; Nguyen, Phuong Thanh; Nguyen, Tung; Nguyen, Tien Ngoc; To, Thanh Long; Nguyen, Tho Dang; Pham, Vu Phong; Le, Vu Tri; Do, Hoa Thi; Vo, Hung Van; Le, Quang Vinh Tin; Tran, Tan Minh; Nguyen, Thanh Duy; Thai, Phuong Duy; Nguyen, Dang Hoang; Le, Anh Quynh Thi; Nguyen, Diep Thi; Uchida, Yuko; Saito, Takehiko
Fonte: J Virol;91(1)2017 Jan 01.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27795418
Resumo: Active surveillance of influenza A viruses of swine (IAV-S) involving 262 farms and 10 slaughterhouses in seven provinces in northern and southern Vietnam from 2010 to 2015 yielded 388 isolates from 32 farms; these viruses were classified into H1N1, H1N2, and H3N2 subtypes. Whole-genome sequencing followed by phylogenetic analysis revealed that the isolates represented 15 genotypes, according to the genetic constellation of the eight segments. All of the H1N1 viruses were entirely A(H1N1)pd (mais)

Dual Roles of the Hemagglutinin Segment-Specific Noncoding Nucleotides in the Extended Duplex Region of the Influenza A Virus RNA Promoter.

Autor(es): Wang, Jingfeng; Li, Jinghua; Zhao, Lili; Cao, Mengmeng; Deng, Tao
Fonte: J Virol;91(1)2017 Jan 01.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27795444
Resumo: We recently reported that the segment-specific noncoding regions (NCRs) of the hemagglutinin (HA) and neuraminidase (NA) segments are subtype specific, varying significantly in sequence and length at both the 3' and 5' ends. Interestingly, we found that nucleotides CC at positions 13 and 14 at the 3' end and GUG at positions 14 to 16 at the 5' end (termed 14' and 16' to distinguish them from 3' positions) are absolutely conserved among all HA subtype-specific NCRs. These HA segment-specific (mais)

Detection of porcine reproductive and respiratory syndrome virus (PRRSV) and influenza A virus (IAV) in oral fluid of pigs.

Autor(es): Biernacka, Kinga; Karbowiak, Pawel; Wróbel, Pawel; Chareza, Tomasz; Czopowicz, Michal; Balka, Gyula; Goodell, Christa; Rauh, Rolf; Stadejek, Tomasz
Fonte: Res Vet Sci;109: 74-80, 2016 Dec.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27892877
Resumo: Recently oral fluid has become a novel sample type for pathogen nucleic acid and antibody detection, as it is easy to obtain with non-invasive procedures. The objective of the study was to analyze porcine reproductive and respiratory syndrome virus (PRRSV) and influenza A virus (IAV) circulation in growing pigs from three Polish production farms, using Real Time PCR and ELISA testing of oral fluid and serum. Oral fluids were collected every 2weeks, in the same 3-4 pens of pigs aged between (mais)

Deep Sequencing of Influenza A Virus from a Human Challenge Study Reveals a Selective Bottleneck and Only Limited Intrahost Genetic Diversification.

Autor(es): Sobel Leonard, Ashley; McClain, Micah T; Smith, Gavin J D; Wentworth, David E; Halpin, Rebecca A; Lin, Xudong; Ransier, Amy; Stockwell, Timothy B; Das, Suman R; Gilbert, Anthony S; Lambkin-Williams, Robert; Ginsburg, Geoffrey S; Woods, Christopher W; Koelle, Katia
Fonte: J Virol;90(24): 11247-11258, 2016 Dec 15.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27707932
Resumo: Knowledge of influenza virus evolution at the point of transmission and at the intrahost level remains limited, particularly for human hosts. Here, we analyze a unique viral data set of next-generation sequencing (NGS) samples generated from a human influenza challenge study wherein 17 healthy subjects were inoculated with cell- and egg-passaged virus. Nasal wash samples collected from 7 of these subjects were successfully deep sequenced. From these, we characterized changes in the subjects (mais)

Cross-Neutralising Nanobodies Bind to a Conserved Pocket in the Hemagglutinin Stem Region Identified Using Yeast Display and Deep Mutational Scanning.

Autor(es): Gaiotto, Tiziano; Hufton, Simon E
Fonte: PLoS One;11(10): e0164296, 2016.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27741319
Resumo: Cross-neutralising monoclonal antibodies against influenza hemagglutinin (HA) are of considerable interest as both therapeutics and diagnostic tools. We have recently described five different single domain antibodies (nanobodies) which share this cross-neutralising activity and suggest their small size, high stability, and cleft binding properties may present distinct advantages over equivalent conventional antibodies. We have used yeast display in combination with deep mutational scanning (mais)

Improving influenza virological surveillance in Europe: strain-based reporting of antigenic and genetic characterisation data, 11 European countries, influenza season 2013/14.

Autor(es): Broberg, Eeva; Hungnes, Olav; Schweiger, Brunhilde; Prosenc, Katarina; Daniels, Rod; Guiomar, Raquel; Ikonen, Niina; Kossyvakis, Athanasios; Pozo, Francisco; Puzelli, Simona; Thomas, Isabelle; Waters, Allison; Wiman, Åsa; Meijer, Adam
Fonte: Euro Surveill;21(41)2016 Oct 13.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27762211
Resumo: Influenza antigenic and genetic characterisation data are crucial for influenza vaccine composition decision making. Previously, aggregate data were reported to the European Centre for Disease Prevention and Control by European Union/European Economic Area (EU/EEA) countries. A system for collecting case-specific influenza antigenic and genetic characterisation data was established for the 2013/14 influenza season. In a pilot study, 11 EU/EEA countries reported through the new mechanism. We (mais)

Reversion of Cold-Adapted Live Attenuated Influenza Vaccine into a Pathogenic Virus.

Autor(es): Zhou, Bin; Meliopoulos, Victoria A; Wang, Wei; Lin, Xudong; Stucker, Karla M; Halpin, Rebecca A; Stockwell, Timothy B; Schultz-Cherry, Stacey; Wentworth, David E
Fonte: J Virol;90(19): 8454-63, 2016 Oct 01.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27440882
Resumo: UNLABELLED: The only licensed live attenuated influenza A virus vaccines (LAIVs) in the United States (FluMist) are created using internal protein-coding gene segments from the cold-adapted temperature-sensitive master donor virus A/Ann Arbor/6/1960 and HA/NA gene segments from circulating viruses. During serial passage of A/Ann Arbor/6/1960 at low temperatures to select the desired attenuating phenotypes, multiple cold-adaptive mutations and temperature-sensitive mutations arose. A substan (mais)

Caspase-mediated degradation of host cortactin that promotes influenza A virus infection in epithelial cells.

Autor(es): Chen, Da-Yuan; Husain, Matloob
Fonte: Virology;497: 146-56, 2016 Oct.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27471953
Resumo: Influenza A virus (IAV) is well-known to exploit host factors to its advantage. Here, we report that IAV exploits host cortactin, an actin filament-stabilising protein for infection in epithelial cells. By using RNA interference-mediated knockdown and overexpression approach, we demonstrate that cortactin promotes IAV infection. However, cortactin polypeptide undergoes the degradation during late IAV infection. By perturbing the lysosome and proteasome, two main compartments governing the d (mais)

Evaluating the potential of a loop-extended scorpion toxin-like peptide as a protein scaffold.

Autor(es): Zhang, Shangfei; Zhu, Limei; Yu, Jie; Xu, Jun; Gao, Bin; Zhou, Changlin; Zhu, Shunyi
Fonte: Protein Eng Des Sel;29(12): 607-616, 2016 Dec.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27672050
Resumo: Grafting of exogenous bioactive sites or functional motifs onto structurally stable scaffolds to gain new functions represents an important research direction in protein engineering. Some engineered proteins have been developed into therapeutic drugs. MeuNaTxα-3 (abbreviated as MT-3) is a newly characterized scorpion sodium channel toxin-like peptide isolated from the venom of the scorpion Mesobuthus eupeus, which contains a rigid scaffold highly similar to classical scorpion sodium ch (mais)

Enhanced Genetic Characterization of Influenza A(H3N2) Viruses and Vaccine Effectiveness by Genetic Group, 2014-2015.

Autor(es): Flannery, Brendan; Zimmerman, Richard K; Gubareva, Larisa V; Garten, Rebecca J; Chung, Jessie R; Nowalk, Mary Patricia; Jackson, Michael L; Jackson, Lisa A; Monto, Arnold S; Ohmit, Suzanne E; Belongia, Edward A; McLean, Huong Q; Gaglani, Manjusha; Piedra, Pedro A; Mishin, Vasiliy P; Chesnokov, Anton P; Spencer, Sarah; Thaker, Swathi N; Barnes, John R; Foust, Angie; Sessions, Wendy; Xu, Xiyan; Katz, Jacqueline; Fry, Alicia M
Fonte: J Infect Dis;214(7): 1010-9, 2016 Oct 01.
MEDLINE - Literatura Internacional em Ciências da Saúde PMID: 27190176
Resumo: BACKGROUND: During the 2014-2015 US influenza season, expanded genetic characterization of circulating influenza A(H3N2) viruses was used to assess the impact of the genetic variability of influenza A(H3N2) viruses on influenza vaccine effectiveness (VE). METHODS: A novel pyrosequencing assay was used to determine genetic group, based on hemagglutinin (HA) gene sequences, of influenza A(H3N2) viruses from patients enrolled at US Influenza Vaccine Effectiveness Network sites. VE was estimate (mais)
Resultados  1-12 de 64