01da descexport-xml.xslid:mdl-2231839420standardxslt12OBJECTIVE: A new vaccine, Multimeric-001, containing conserved linear epitopes from the HA, NP, and M1 proteins of influenza type A and type B strains was designed to protect against seasonal and pandemic influenza virus strains, regardless of mutations. We assessed its safety and tolerability and characterized humoral and cellular immune responses elicited by its administration. METHODS: Sixty healthy volunteers received either 250 or 500 µg injections, with or without adjuvant (Montanide ISA 51VG), or matching placebo. Two intramuscular injections were administered, 21 days apart. RESULTS: Treatment was well tolerated and no significant adverse events were noted. Forty-two days after first injection, there was a 50-fold and 37-fold increase in IgG titers against Multimeric-001 protein, following the adjuvanted 500 and 250 µg doses, respectively. Sera from immunized subjects lysed MDCK cells infected with strains of influenza representing the major strains that infect humans: H1N1, H3N2, and influenza B. Proliferation of peripheral blood mononuclear cells as well as increase in IL-2 and IFN-gamma secretion occurred following incubation with the vaccine. CONCLUSION: This vaccine model differs fundamentally from the current influenza virus vaccines, as it does not contain the variable regions of the virus hemagglutinin and hence does not induce hemagglutination inhibition antibodies that serve as surrogate markers for protection. In order to demonstrate the potential efficacy of the Multimeric-001, an alternative assay was employed, in which the lysis of MDCK cells infected with different virus strains was shown, with the involvement of the complement mechanism. The humoral and cellular responses suggest a cross-immunity of the vaccine toward influenza virus strains regardless of mutations. These results corroborate the protective effect of the vaccine, previously shown in animals. Larger-scale studies are under way to further substantiate the safety and efficacy of the vaccine candidate.Atsmon JKate-Ilovitz EShaikevich DSinger YVolokhov IHaim KYBen-Yedidia Th1n1Netherlands201205MEDLINEJ Clin Immunol;32(3): 595-603, 2012 Jun. mdl-223183943enAdjuvantes Imunológicos/administração & dosagemEpitopos/administração & dosagemVírus da Influenza A/imunologiaVírus da Influenza B/imunologiaVacinas contra Influenza/administração & dosagemManitol/análogos & derivadosÁcidos Oléicos/administração & dosagemAdolescenteAdultoAnimaisAntígenos Virais/imunologiaLinhagem CelularCãesFemininoGlicoproteínas de Hemaglutininação de Vírus da Influenza/imunologiaHumanosImunoglobulina G/imunologiaInterferon gama/imunologiaInterleucina-2/imunologiaLeucócitos Mononucleares/imunologiaMasculinoManitol/administração & dosagemMeia-IdadeNucleoproteínas/imunologiaProteínas da Matriz Viral/imunologiaAdulto Jovem00.6522012595-603Ensaio Clínico Fase IEnsaio Clínico Fase IIArtigo de RevistaEnsaio Clínico Controlado AleatórioResearch Support, Non-U.S. Gov'tJ Clin ImmunolSafety and immunogenicity of multimeric-001--a novel universal influenza vaccine.articlehttp://dx.doi.org/10.1007/s10875-011-9632-532111111111111