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Genetic-relatedness of peri-implants and buccal Candida albicans isolates determined by RAPD-PCR / Relación genética de aislamentos de candida albicans por rapd-pcr en surcos en surcos peri-implantarios de cavidad bucal

Bertone, Adriana Mabel; Rosa de Nastri, Alcira Cristina; Nastri, Natalia Lorena; Santillán, Héctor D; Ariza, Yamila; Lovannitti, Cristina A; Jewtuchowicz, Virginia Marta.
Acta odontol. latinoam ; 29(3): 197-205, 2016. tab, graf
Artigo em Inglês | LILACS | ID: biblio-868691
Molecular techniques have been used in recent studies toidentify a wide range of potential bacterial pathogens inperi­implant pockets of the oral cavity. However, the prevalence and molecular epidemiology of yeasts and species distribution related to peri­implantitis are as yet unknown. The aim of thisstudy was to determine the prevalence and distribution of yeasts in peri­-implant biofilm and to study genetic relatedness of Candida albicans.Yeasts recovered from peri­implant biofilm samples (n=89) andbuccal samples (n=120) were studied in 40 immunocompetent non­-smoking patients who visited the dental clinic of the Asociación Implantodontológica Argentina, Buenos Aires, Argentina, and had received oral rehabilitation with implants for more than five years. Yeasts recovered from samples were studied by typing assays using RAPD­PCR. The prevalence of yeasts in the peri­implant sulcus was 73% (n=29). C. albicans was the most prevalent species identified in this study population. The RAPD analysis showed identical genotypes inmost C. albicans spp. from the two different sampling sites buccal and peri­implant. These findings suggest that periimplant biofilm is an ecological niche that favors the growth of yeast species. Most C. albicans found in peri­implant biofilmoriginate from the endogenous infection caused by commensalstrains.
Biblioteca responsável: AR29.1