RESUMO
Cicatrization can be divided into three phases: inflammation, fibroblastic, maturation and remodeling [1]. The extracellular matrix may be replaced by a stronger and more elastic connective tissue. In a scar collagen is the major component of the mature connective tissue [2]. In homeopathic area, the greater is the investigated segment ultra dilutions [6]. However, little research has been done to explore the effect of dynamized drugs in in vitro cell culture [4]. Using the Zincum metallicum 6CH and Calendula officinalis 6CH applied independently in different concentrations in fibroblast cultures sought to determine the increase in proliferative activity using techniques such as IC50, MTT, flow cytometry and quantification of collagen. As expected from the literature, ie both homeopathic according to the literature are used for treatments that Require skin healing, both showed increased proliferative activity, having Calendula most cellular response, presenting as cell cycle stimulating checked via flow cytometry.(AU)
Assuntos
Humanos , Cicatrização , Zincum Metallicum/uso terapêutico , /uso terapêutico , Altas Potências , FibroblastosAssuntos
Humanos , Animais , Cobaias , Técnicas In Vitro , Doses Mínimas , Pesquisa Homeopática Básica , Mecanismo de Ação do Medicamento Homeopático , Calorimetria , Técnicas de Cultura , Intoxicação por Cádmio , Cisplatino/envenenamento , Anuros , Tiroxina/farmacologia , /farmacologia , Fator de Crescimento Epidérmico/farmacologia , Fibroblastos , Cólera/terapia , Medicina Herbária , Galphimia/uso terapêutico , Rinite Alérgica Sazonal/terapia , Ensaios Clínicos como Assunto , Triticum , Argentum Nitricum/farmacologia , Conformação Molecular , Adjuvantes Imunológicos , Carcinógenos , Neoplasias/terapiaRESUMO
The effects of several preparations of Iscador, a commercially obtainable extract of mistletoe, Viscum album L., on cell proliferation and cell viability was studied. Iscador Quercus was shown to inhibit cell proliferation of: human fibroblast cell lines, mouse tumour cell lines, human carcinoma tumour cell lines and a human lymphoblastic tumour cell line. Iscador Quercus, Iscador Ulnus and Iscador Malus were about equally cytostatic to mouse 3T3 fibroblasts. Iscador Pinus was somewhat less cytostatic. Iscador Quercus had a very direct cytotoxic effect on MOLT4 tumour cells as assessed by trypan blue dye exclusion. The cytostatic and cytotoxic properties of Iscador could be annulled by heating Iscador form 30 min. at 90 "graus"C. Iscador reduced the cloning efficiency of ASG experiments it can be concluded that a small percentage of CHO cells is insensitive to high concentrations of Iscador at short incubation times. The importance of these findings in relation to the cytostatic and cytotoxic compounds possibly present in Iscador is discussed
