RESUMO
The aim of the present study was to isolate novel lactic acid bacteria (LAB) from hairy vetch forage crop and characterize their probiotic and fermentative potential for preparing Korean cabbage kimchi. First, functional characterization of isolated strains such as antagonistic property, auto-aggregation, antibiotic susceptibility, and extracellular enzyme production was performed. The isolated Lactobacillus plantarum KCC-41 strain was able to inhibit pathogenic fungal spore formation. It showed susceptibility to common commercial antibiotics drugs. The selected LAB strain was then subjected to microencapsulation with alginate biopolymer. Its ability to survive in in vitro simulated gastro-intestinal fluid was evaluated. It was also used in the fermentation of cabbage kimchi samples. The encapsulated KCC-41 strain could effectively lead to kimchi fermentation in terms of reducing its pH and dominating bacterial count. It also significantly increased organic acid production than non-encapsulated LAB (KCC-41) for cabbage kimchi samples
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Assuntos
Brassica/microbiologia , Células Imobilizadas/metabolismo , Microbiologia de Alimentos , Probióticos/metabolismo , Endófitos/isolamento & purificação , Lactobacillus plantarum/isolamento & purificação , Vicia/microbiologia , Composição de Medicamentos , Endófitos/metabolismo , Lactobacillus plantarum/metabolismoRESUMO
La microencapsulación de células es una estrategia terapéutica que permite el tratamiento de un gran número de enfermedades crónicas sin la necesidad de agentes inmunosupresores. Para lograr este objetivo es necesario inmovilizar células secretoras de productos terapéuticos en microcápsulas convenientemente diseñadas, de forma que se asegure la funcionalidad del injerto a largo plazo. Los avances realizados en este campo, a lo largo de los últimos años, auguran que la microencapsulación de células pueda convertirse, en un futuro no muy lejano, en una estrategia terapéutica de uso clínico habitual (AU)
Assuntos
Humanos , Composição de Medicamentos/métodos , Cápsulas/farmacologia , Biotecnologia/tendências , Células Imobilizadas/análise , Enzimas Imobilizadas/análise , Linhagem CelularRESUMO
Immobilized lichen cells from the thalli of the lichen Pseudevernia furfuracea, supplied with acetate as the only source of carbon, continuously produced phenolic substances, atranorin and physodic acid, over 23 days. Epiphytic bacteria associated with the lichen thallus grew actively, probably using both acetate and reduced compounds supplied by lichen cells, since their active growth was avoided by including 10 microM 3,3'-dichlorophenyl-1,1'dimethylurea in the bath solution. Penicillin largely impeded the growth of epiphytic bacteria and decreased phenolic production, which was recovered only at the end of the experimental period, just when the bacteria started a slow, but active growth. We suggest the cooperation of epiphytic bacteria in the biosynthesis of both atranotrin and physodic acid (AU)
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