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1.
Rev. esp. patol ; 56(4): 279-283, Oct-Dic, 2023. tab, ilus, graf
Artigo em Inglês | IBECS | ID: ibc-226961

RESUMO

Intranodal palisaded myofibroblastoma (IPM) is a rare stroma-derived spindle-cell neoplasm of the lymph node with myofibroblastic differentiation and CTNNB1 (β-catenin gene) somatic mutations. We present a case of IPM found incidentally in the staging of lung adenocarcinoma. We describe the major histopathological and phenotypic features, including a palisaded bland spindle cell proliferation with myofibroblastic differentiation and Wnt pathway activation by immunohistochemistry, including β-catenin expression. Production of osteoid-like collagen directly from tumor cells was observed. We confirmed p.Gly34Arg CTNNB1 mutation by direct sequencing. We also reviewed the literature for similar cases.(AU)


El miofibroblastoma en empalizada intraganglionar linfático (MEIG) es una neoplasia infrecuente de células fusiformes del estroma del ganglio linfático con diferenciación miofibroblástica y mutaciones en CTNNB1 (gen de la β-catenina). Aquí mostramos el caso de un paciente con MEIG encontrado incidentalmente en la estadificación por un adenocarcinoma de pulmón. Se describen las características histopatológicas principales de la entidad, incluyendo una proliferación de células fusiformes con escasa atipia, empalizadas celulares y diferenciación miofibroblástica con activación de la vía Wnt, incluyendo expresión inmunohistoquímica de β-catenina. Se observó producción de colágeno de tipo osteoide por parte de las células tumorales. Se confirmó la presencia de la mutación p.Gly34Arg de CTNNB1 mediante secuenciación directa. Se recogen adicionalmente publicaciones de casos similares al nuestro.(AU)


Assuntos
Humanos , Feminino , Idoso , Mutação , Neoplasias de Tecido Muscular , Células Estromais , Via de Sinalização Wnt , beta Catenina , Neoplasias Pulmonares , Patologia Clínica , Pacientes Internados , Exame Físico , Avaliação de Sintomas
2.
Med. oral patol. oral cir. bucal (Internet) ; 28(6): e512-e518, nov. 2023. tab, ilus, graf
Artigo em Inglês | IBECS | ID: ibc-227368

RESUMO

Background: Oral Lichen Planus is a potential malignant disorder and shares clinical and histopathological features with other similar lesions. ALDH1 is a specific biomarker for stem cells identification, however its role in stromal cells of immune inflammatory infiltrate has not been explored. The aim of this study was to investigate the ALDH1 immunoexpression in epithelial and stromal cells of Oral Lichen Planus and other lesions with lichenoid inflammatory infiltrate. Material and Methods: 64 samples of Oral Lichen Planus, Oral Lichenoid Lesions, Oral Leukoplakia and Unspecific Chronic Inflammation were included. ALDH1 was evaluated in both epithelium and stromal cells. ALDH1+ cells ≥ 5% were considered positive in epithelium. Stromal cells were evaluated semi quantitatively. Fields were ranked in scores, according to criteria: 1 (0 to 10%); 2 (11 to 50%) and 3 (>50%). The mean value of the sum of the fields was the final score. Statistical differences among groups were investigated, considering p < 0.05. Results: ALDH1 expression in epithelium was low in all groups without difference among them. ALDH1+ cells in the lamina propria were higher for Lichen Planus [2.0], followed by Leukoplakia [1.3], Lichenoid lesions [1.2] and control [1.1] (p<0.05). Conclusions: ALDH1 immunoexpression in epithelium of lichenoid potential malignant disorders did not show a contributory tool, however ALDH1 in stromal cells of lichen planus might be involved in the complex process of immune regulation associated with the pathogenesis of this disease. (AU)


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Erupções Liquenoides/patologia , Líquen Plano Bucal/patologia , Estudos Transversais , Epitélio/patologia , Células Estromais/patologia
3.
Clin. transl. oncol. (Print) ; 25(2): 333-344, feb. 2023.
Artigo em Inglês | IBECS | ID: ibc-215933

RESUMO

Metastasis is the leading cause of mortality related to cancer. In the course of metastasis, cancer cells detach from the primary tumor, enter the circulation, extravasate at secondary sites, and colonize there. All of these steps are rate limiting and decrease the efficiency of metastasis. Prior to their arrival, tumor cells can modify the secondary sites. These favorable microenvironments increase the probability of successful dissemination and are referred to as pre-metastatic niches. Cancer cells use different mechanisms to induce and maintain these niches, among which immune cells play prominent roles. The immune system, including innate and adaptive, enhances recruitment, extravasation, and colonization of tumor cells at distant sites. In addition to immune cells, stromal cells can also contribute to forming pre-metastatic niches. This review summarizes the pro-metastatic responses conducted by immune cells and the assistance of stromal cells and endothelial cells in the induction of pre-metastatic niches (AU)


Assuntos
Humanos , Células Endoteliais/patologia , Metástase Neoplásica/patologia , Células Estromais/patologia , Carcinogênese/patologia , Microambiente Tumoral
4.
Med. oral patol. oral cir. bucal (Internet) ; 27(4): 1-9, July 2022. tab, graf, ilus
Artigo em Inglês | IBECS | ID: ibc-209793

RESUMO

Background: Analysis of the tumor microenvironment has been proposed as a strategy for the treatment and prognosis of different neoplastic processes. A grading system based on the tumor-stroma ratio (TSR), which evaluates theproportion of stroma in relation to neoplastic parenchyma at the invasion front, has shown a strong prognostic valuein different neoplastic processes. The aim of the present systematic review was to understand the role of the TSR inhead and neck squamous cell carcinoma (HNSCC), evaluating its correlation with clinical and prognostic parameters.Material and Methods: An electronic search was performed in PubMed/Medline, Web of Science, Science Direct,Scopus, Embase, and the Cochrane Collaboration Library. Publications assessing the relationship between TSRand prognosis in cases of HNSCC were eligible. The quality of the studies was assessed independently by fourevaluators using the Newcastle-Ottawa scale.Results: After application of the previously es+lished inclusion/exclusion criteria, nine articles were included inthe qualitative synthesis. With regards to quality on the Newcastle-Ottawa scale, an overall value of 4.55 was obtained. This systematic review demonstrated a strong association between TSR and prognosis in esophageal andoral squamous cell carcinomas.Conclusions: Histopathological analysis of the TSR can optimize the analysis of the prognosis of cases diagnosedwith HNSSC. In addition, the TSR is a reliable and simple parameter that can be evaluated in hematoxylin/eosinstained slides during routine laboratory examinations, showing high inter- and intraobserver agreement. (AU)


Assuntos
Humanos , Carcinoma de Células Escamosas/patologia , Neoplasias de Cabeça e Pescoço/patologia , Prognóstico , Carcinoma de Células Escamosas de Cabeça e Pescoço/patologia , Células Estromais/patologia , Microambiente Tumoral
5.
Cient. dent. (Ed. impr.) ; 18(3): 159-164, jun.-jul. 2021. ilus, tab
Artigo em Espanhol | IBECS | ID: ibc-217147

RESUMO

Actualmente, la implantología está considerada como la terapia de elección para rehabilitar dientes ausentes. Sin embargo, debido a diversos factores, no siempre se consigue un enfoque inmediato para la colocación de los implantes. Se debe prestar una atención especial a los sectores maxilares posteriores, que debido al paso del tiempo y a la ausencia de dientes, sufren una gran atrofia alveolar que se encuentra asociada a una expansión del seno maxilar que dificulta, en ocasiones, la rehabilitación con implantes osteointegrados. Debido a esta pérdida ósea, se han desarrollado técnicas quirúrgicas como la elevación de seno maxilar para obtener una ganancia de hueso y poder favorecer la colocación y el pronóstico de los implantes. Sin embargo, existen situaciones en las que incluso esta técnica no aporta un volumen óseo suficiente, siendo preciso acompañarla de técnicas de regeneración ósea.En la actualidad, existen estudios que plantean el uso de células madre mesenquimales en procedimientos de regeneración ósea como alternativa a los procedimientos convencionales.El objetivo del presente trabajo es evaluar a través de la realización de una revisión sistemática de la literatura, la efectividad de las células madres mesenquimales en elevaciones de seno maxilar con diferentes tipos de matriz ósea. (AU)


Currently, implantology is considered the therapy of choice to rehabilitate missing teeth. However, due to various factors, we do not always get an immediate approach to the placement of our implants. Above all, we must pay special attention to the posterior maxillary sectors, which due to the passage of time and the absence of dental pieces suffer great alveolar atrophy associated with an expansion of the maxillary sinus that makes difficult and sometimes impossible the implant therapy. Due to this great bone loss, the professionals have been forced to practice surgical techniques such as maxillary sinus lift to gain bone and to be able to favour the placement and prognosis of the implants. However, there are clinical situations in which even this technique does not give us sufficient bone volume, being necessary a bone regeneration therapy.Thus, thanks to advances in the field of scientific research, various methods have been used to solve this type of problem, but one of the most innovative and current options is bone regeneration using mesenchymal stem cells.This work aims to evaluate, carrying out a systematic review of the literature, the effectiveness of mesenchymal stem cells in maxillary sinus elevations with several types of matrix. (AU)


Assuntos
Humanos , Células-Tronco Mesenquimais , Levantamento do Assoalho do Seio Maxilar , Regeneração Óssea , Medula Óssea , Células Estromais
6.
Arch. bronconeumol. (Ed. impr.) ; 57(2): 130-137, feb. 2021. tab, graf, ilus
Artigo em Inglês | IBECS | ID: ibc-200894

RESUMO

BACKGROUND: Stroma, mainly composed by fibroblasts, extracellular matrix (ECM) and vessels, may play a role in tumorigenesis and cancer progression. Chronic Obstructive Pulmonary Disease (COPD) is an independent risk factor for LC. We hypothesized that markers of fibroblasts, ECM and endothelial cells may differ in tumors of LC patients with/without COPD. METHODS: Markers of cultured cancer-associated fibroblasts and normal fibroblasts [CAFs and NFs, respectively, vimentin and alpha-smooth muscle actin (SMA) markers, immunofluorescence in cultured lung fibroblasts], ECM, and endothelial cells (type I collagen and CD31 markers, respectively, immunohistochemistry) were identified in lung tumor and non-tumor specimens (thoracotomy for lung tumor resection) from 15 LC-COPD patients and 15 LC-only patients. RESULTS: Numbers of CAFs significantly increased, while those of NFs significantly decreased in tumor samples compared to non-tumor specimens of both LC and LC-COPD patients. Endothelial cells (CD31) significantly decreased in tumor samples compared to non-tumor specimens only in LC patients. No significant differences were seen in levels of type I collagen in any samples or study groups. CONCLUSIONS: Vascular endothelial marker CD31 expression was reduced in tumors of non-COPD patients, while type I collagen levels did not differ between groups. A rise in CAFs levels was detected in lung tumors of patients irrespective of airway obstruction. Low levels of CD31 may have implications in the overall survival of LC patients, especially in those without underlying airway obstruction. Identification of CD31 role as a prognostic and therapeutic biomarker in lung tumors of patients with underlying respiratory diseases warrants attention


ANTECEDENTES: El estroma, compuesto principalmente por fibroblastos, matriz extracelular (MEC) y vasos, puede desempeñar un papel en la génesis tumoral y la progresión del cáncer. La enfermedad pulmonar obstructiva crónica (EPOC) es un factor de riesgo independiente para el carcinoma de pulmón (CP). Nuestra hipótesis fue que los marcadores de fibroblastos, MEC y células endoteliales pueden variar en los tumores de los pacientes con CP con o sin EPOC. MÉTODOS: Se identificaron los marcadores de fibroblastos asociados al cáncer y los fibroblastos normales cultivados (FAC y FN, respectivamente; marcadores: vimentina y α-actina del músculo liso [SMA por sus siglas en inglés]; inmunofluorescencia en fibroblastos de pulmón cultivados) y marcadores de la MEC y las células endoteliales (marcadores: colágeno tipo I y CD31, respectivamente; inmunohistoquímica) en muestras de pulmón tumoral y no tumoral (toracotomía para resección de tumores pulmonares) de 15 pacientes con EPOC-CP y 15 pacientes con solo CP. RESULTADOS: El número de FAC aumentó de forma significativa, mientras que el de FN disminuyó significativamente en las muestras tumorales en comparación con las muestras no tumorales de pacientes con CP y EPOC-CP. Las células endoteliales (CD31) disminuyeron también de forma significativa en las muestras tumorales en comparación con las muestras no tumorales solo en los pacientes con CP. No se observaron diferencias significativas en los niveles de colágeno tipo I en ninguna muestra o grupo de estudio. CONCLUSIONES: La expresión del marcador vascular endotelial CD31 se redujo en los tumores de los pacientes sin EPOC, mientras que los niveles de colágeno tipo I no difirieron entre los grupos. Se detectó un aumento en los niveles de FAC en los tumores de pulmón de los pacientes, con independencia de la presencia de obstrucción de las vías respiratorias. Los niveles bajos de CD31 pueden tener implicaciones en la supervivencia general de los pacientes con CP, en especial, en aquellos sin obstrucción subyacente de las vías respiratorias. Convendría estudiar e identificar el papel del CD31 como biomarcador terapéutico y de pronóstico en los tumores de pulmón de pacientes con enfermedades respiratorias subyacentes


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Idoso , Neoplasias Pulmonares/patologia , Matriz Extracelular/patologia , Fibroblastos Associados a Câncer/patologia , Doença Pulmonar Obstrutiva Crônica/patologia , Estudos Transversais , Estudos Prospectivos , Molécula-1 de Adesão Celular Endotelial a Plaquetas/análise , Colágeno Tipo I/análise , Progressão da Doença , Biomarcadores Tumorais/análise , Actinas/análise , Imuno-Histoquímica , Células Estromais/patologia , Carcinogênese
8.
Rev. esp. patol ; 52(3): 154-162, jul.-sept. 2019. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-191931

RESUMO

We investigated the efficiency and accuracy of endoscopic ultrasound-guided fine-needle aspiration cytology (EUS-FNAC) in the diagnosis of gastrointestinal leiomyoma (GIL). Between January 2009 and May 2018 we performed 795 EUS-FNAC studies of lesions of the gastrointestinal (GI) tract for various clinical indications. A diagnosis of GIL by cytological and cell block study was made in 14 patients (57.1% males, mean age 53.6 years, range 22-84 years).7 tumors (50%) were detected incidentally. The lesions ranged in size from 2 to 10cm (mean size 4.4cm). The location of the tumors was: esophagus 7 (50%), stomach 6 (42.9%) and rectum 1(7.1%). The mean size of the symptomatic tumors was 5.2cm (range 3-10cm). The follow-up of the 14 patients varied from 1 to 108 months (median 39.5 months), during which no recurrence or evidence of lesion progression was observed. Imaging alone was not sufficient for an accurate diagnosis to be made. The pathological diagnosis was based on a combination of cytological, histopathological, and immunohistochemical features. The intracytoplasmic eosinophilic globule is a useful marker of paucicellular GIL differentiating it from gastrointestinal stromal tumor and leiomyosarcoma. EUS-FNAC is a reliable, accurate, and safe method for the diagnosis of GIL


Hemos investigado la eficacia y la precisión de la citología por aspiración con aguja fina guiada por ecografía endoscópica (EUS-FNAC) en el diagnóstico del leiomioma gastrointestinal (LGI). Entre enero de 2009 y mayo de 2018 se realizaron 795 estudios EUS-FNAC de lesiones del tracto digestivo por una variedad de indicaciones clínicas. Catorce pacientes (57,1% varones, edad media: 53,6 años, rango: 22-84 años) fueron diagnosticados mediante estudio citológico y de bloque celular de LGI. Siete tumores (50%) fueron detectados de manera incidental. Las lesiones variaron en tamaño de 2 a 10cm (tamaño promedio: 4,4cm). La localización de los tumores fue: esófago 7 (50%), estómago 6 (42,9%) y recto uno (7,1%). El tamaño medio de los tumores sintomáticos fue de 5,2cm (rango: 3-10cm). El seguimiento de los 14 pacientes varió de uno a 108 meses (mediana: 39,5 meses). No se observó recurrencia o evidencia de progresión de la lesión. El estudio de las imágenes radiológicas por sí solo no permitió diagnosticar la lesión. El diagnóstico patológico se basó en una combinación de datos citológicos, histopatológicos e inmunohistoquímicos. El glóbulo eosinofílico intracitoplasmático es un marcador útil de LGI paucicelular que permite diferenciarlo del tumor del estroma gastrointestinal y del leiomiosarcoma. La EUS-FNAC es un método fiable, preciso y seguro para el diagnóstico del LGI


Assuntos
Humanos , Masculino , Feminino , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Aspiração por Agulha Fina Guiada por Ultrassom Endoscópico/métodos , Leiomioma/patologia , Neoplasias Gastrointestinais/patologia , Células Estromais/patologia , Leiomiossarcoma/patologia , Mesenquimoma/patologia
9.
Rev. senol. patol. mamar. (Ed. impr.) ; 32(1): 17-25, ene.-mar. 2019. ilus, graf
Artigo em Espanhol | IBECS | ID: ibc-187029

RESUMO

A pesar de los avances conseguidos en el tratamiento del cáncer de mama, todavía sigue siendo una causa importante de mortalidad en las mujeres. Por tanto, resulta necesario plantear nuevos enfoques de la fisiopatología de la enfermedad que contribuyan a realizar una mejor evaluación pronóstica, y a la mejora de las estrategias terapéuticas. Para ello, deberíamos considerar que el cáncer no es solo una transformación maligna de las células epiteliales y su progresión meramente autónoma; sino que, hoy en día, existen datos que apoyan el concepto del cáncer como un ecosistema basado en una sociología de diferentes tipos celulares, con sus interacciones complejas. Entre los diversos tipos de células que conforman el estroma tumoral, y que tienen un papel relevante en la progresión del cáncer de mama, se encuentran los fibroblastos asociados al cáncer, las células inflamatorias y las células endoteliales. Existen diferentes factores moleculares expresados por esas células que se asocian con el desarrollo de metástasis, tales como las metaloproteasas de matriz y sus inhibidores tisulares, citoquinas o receptores tipo toll. En base a la expresión de todos ellos, aquí proponemos 2 fenotipos de estroma del cáncer de mama con influencias marcadamente diferentes sobre el pronóstico de las pacientes. También analizamos los mecanismos involucrados en la interrelación tumor-estroma que pueden llevarnos a mejorar las estrategias terapéuticas en el cáncer de mama


Despite advances in the treatment of breast cancer, it remains an important cause of mortality in women. Therefore, it is necessary to propose new approaches to the pathophysiology of the disease that could help to improve prognostic evaluation and therapeutic strategies. To do this, we should consider that cancer is not only a malignant transformation of the epithelial cells or their purely autonomous growth; nowadays, there are data that support the concept of cancer as a system based on a sociology of different cell types, with complex interactions. Among the various types of cells that make up the tumour stroma and which play an important role in the progression of breast cancer are cancer-associated fibroblasts, inflammatory cells and endothelial cells. Several molecular factors expressed by these cells are associated with the development of metastases, such as matrix metalloproteases and their tissue inhibitors, cytokines or toll-like receptors. Based on the expression of all of these factors, here we propose two types of stroma from breast cancer that display markedly different influences on patient prognosis. We also analyse mechanisms involved in the tumour-stroma interrrelationship that could help to improve therapeutic strategies in breast cancer


Assuntos
Humanos , Células Estromais/patologia , Neoplasias da Mama/patologia , Fibroblastos/patologia , Células Endoteliais/patologia , Mediadores da Inflamação/análise , Prognóstico , Receptores Toll-Like/análise , Junções Intercelulares/patologia
10.
Clin. transl. oncol. (Print) ; 20(9): 1185-1195, sept. 2018. ilus, tab, graf
Artigo em Inglês | IBECS | ID: ibc-173704

RESUMO

Purpose: Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers worldwide, and its outcome is poor. The purpose of this study was to determine the association between JNK1 and vitamin D receptor (VDR) expression and the prognosis of ESCC. Methods: Immunohistochemical staining was conducted on ESCC tissue microarrays (362 pairs of ESCC and normal esophagus tissues). The epithelial and stromal expression levels of c-jun NH2-terminal kinase 1 (JNK1) and VDR were scored and correlated with the ESCC characteristics. Laser-capture-based quantitative RT-PCR was performed on ESCC tissues. The effects of JNK1 and VDR on ESCC cell proliferation and migration were analyzed in vitro by transient transfection, and protein changes were evaluated by immunoblotting. Results: Both JNK1 and VDR were reduced in ESCC epithelial cells in comparison with the normal esophagus, but the expression of JNK1 and VDR in ESCC stromal tissues, not epithelial cells, was strongly associated with the survival time of ESCC patients. Functional studies showed that increased JNK1 suppressed cancer cell proliferation, mobility, and migration, which were linked to the alterations of VDR and metastasis-associated proteins. Conclusion: JNK1 and VDR act as tumor suppressors, and their stromal expression levels are associated with prognosis in esophageal squamous cell carcinoma


No disponible


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Células Estromais/patologia , Neoplasias Esofágicas/patologia , Carcinoma de Células Escamosas/patologia , Proteínas Quinases JNK Ativadas por Mitógeno/isolamento & purificação , Receptores de Calcitriol/isolamento & purificação , Prognóstico , Biomarcadores Tumorais/análise , Proteínas Supressoras de Tumor/isolamento & purificação
12.
Cir. plást. ibero-latinoam ; 43(4): 381-386, oct.-dic. 2017. graf, tab
Artigo em Espanhol | IBECS | ID: ibc-170453

RESUMO

Introducción y Objetivo. El tejido adiposo es una fuente de células estromales de fácil acceso. Para su almacenamiento y posterior aplicación en clínica es importante que el método de criopreservación a utilizar mantenga adecuadas tasas de viabilidad tras un ciclo de congelación. El objetivo del presente trabajo es obtener células estromales humanas derivadas del tejido adiposo, implementar un protocolo de criopreservación libre de proteína animal y medir las tasas de viabilidad posterior a un ciclo de criopreservación para su eventual aplicación clínica. Material y Método. Utilizamos grasa fresca de 5 pacientes sometidas a lipoaspiración y aislamos las células estromales mediante técnicas de digestión enzimática y expansión celular. Realizamos la caracterización de las células mediante inmunofenotipificación. Criopreservamos las células utilizando dimetil-sulfóxido 10% y las almacenamos durante 1 mes en nitrógeno líquido. Evaluamos la tasa de viabilidad mediante ioduro de propidio y citometría de flujo, antes y después de un ciclo de criopreservación. Resultados. Obtuvimos células estromales derivadas del tejido adiposo, confirmado con el panel de inmunofenotipificación. Las tasas de viabilidad promedio obtenidas con ioduro de propidio fue 61.89% mientras que la tasa de mortalidad fue 32.68% tras un ciclo de criopreservación. Conclusiones. Mediante un protocolo de criopreservación utilizando un medio definido con dimetil-sulfóxido 10% en ausencia de proteína animal, es posible obtener tasas aceptables de viabilidad de las células estromales derivadas del tejido adiposo tras un ciclo de criopreservación (AU)


Background and Objective. Adipose tissue is an easy access source of stromal cells. For storage and subsequent clinical application, it is important that the method of cryopreservation used maintain adequate viability rates after a cycle of freezing. Our aim is to get stromal cells derived from human adipose tissue, implement a protocol of cryopreservation free of animal protein and subsequent measure viability rates after a cryopreservation cycle for its posterior clinical use. Methods. Fresh adipose tissue of 5 patients undergoing liposuction was used and stromal cells were isolated by enzymatic digestion techniques and cell expansion. Characterization of the cells was performed by immunophenotyping. Cells using 10% dimethylsulfoxide and stored for 1 month were cryopreserved in liquid nitrogen. Viability rates were assessed by propidium iodide and flow cytometry before and after a cryopreservation cycle. Results. Stromal cells derived from adipose tissue, confirmed immunophenotyping panel, were obtained. The average viability rates obtained with propidium iodide was 61.89% while the mortality rate was 32.68% after a cryopreservation cycle. Conclusions. Using a cryopreservation protocol with a defined medium with 10% dimethylsulfoxide and animal protein free, it is possible to obtain acceptable viability rates of stromal cells derived from adipose tissue after a cryopreservation cycle (AU)


Assuntos
Humanos , Feminino , Adulto , Células Estromais , Tecido Adiposo/cirurgia , Transplante de Células-Tronco/métodos , Gordura Abdominal/cirurgia , Imunofenotipagem/métodos , Citometria de Fluxo/métodos , Índice de Massa Corporal
13.
Rev. esp. patol ; 50(4): 229-233, oct.-dic. 2017. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-166038

RESUMO

Recientemente se ha descrito un tumor placentario que se ha denominado corangiocarcinoma. Consiste en una proliferación vascular, con células estromales, rodeadas por una neoplasia trofoblástica atípica. La proliferación vascular y estromal se pone de manifiesto con CD-34 y vimentina. Las células trofoblásticas atípicas, pleomórficas con frecuentes mitosis son positivas para CK 8-18, panCK y BHCG. Es difícil distinguir de un tumor de colisión del tipo corangioma-coriocarcinoma. Hasta el momento actual se han descrito menos de diez casos de esta entidad en la literatura. Tras el seguimiento realizado en cada caso no se han observado metástasis en las progenitoras ni en los descendientes (AU)


Chorangiocarcinoma is a recently described placental tumour showing a vascular proliferation with stromal cells surrounded by an atypical trophoblastic neoplasia. The stromal and vascular proliferation is demonstrated with CD-34 and Vimentin. Atypical pleomorphic trophoblastic cells with frequent mitosis are positive for CK-8-18, pan CK and BCHG. It is difficult to differentiate this carcinoma from a collision tumour of the corangioma-choriocarcinoma type. To date, less than ten cases of this tumour have been reported but follow ups did not reveal any metastases in either mother or child in any case (AU)


Assuntos
Humanos , Feminino , Adulto , Doença Trofoblástica Gestacional/patologia , Células Estromais/patologia , Tumores do Estroma Endometrial/patologia , Coriocarcinoma/patologia , Placenta/patologia , Hemorragia Uterina/complicações , Hemorragia Uterina/patologia , Programas de Rastreamento/métodos , Mitose
14.
Actas urol. esp ; 41(6): 376-382, jul.-ago. 2017. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-164453

RESUMO

Objetivo: Analizar el comportamiento de la metaloprotesa 11 (MMP11) en fibroblastos cultivados procedentes de tumores prostáticos humanos con diferentes características clinicopatológicas. Material y métodos: Para este estudio se analizaron muestras de biopsias de próstata obtenidas por vía transrectal de tumores con diferentes características, tratados o no con privación androgénica (PA). Tras la optimización del método de cultivo, se aislaron y cultivaron los fibroblastos para realizar el estudio (PCR) del ARNm de MMP11. Resultados: Se estudiaron finalmente 37 casos: 5 muestras de hiperplasia benigna de próstata, 14 casos con neoplasias localizadas (7 de alto riesgo según la clasificación de D’Amico), 5 con tumores con metástasis óseas y 13 tratados con PA, de los que 6 cumplían los requisitos para ser definidos como resistentes a la castración. En los tumores sin PA, la expresión de MMP11 fue significativamente mayor (p = 0,001) en los fibroblastos de tumores de grados más altos. Se encontró una correlación significativa (p = 0,001) entre PSA y expresión de MMP11 fibroblástica y un aumento significativo de la expresión de MMP11 en los tumores metastásicos. En los tumores con PA se objetivó una expresión significativamente mayor de MMP11 en pacientes resistentes a la castración que en los sensibles a esta (p = 0,003). Conclusión: En tumores de próstata avanzados o en fases de mayor agresividad tumoral, la producción de MMP11 por los fibroblastos resulta significativamente mayor que en tumores no metastásicos o en fases de sensibilidad a la PA


Objective: To analyze the expression of metalloprotein 11 (MMP11) in cultured fibroblasts obtained from human prostate tumors with different clinical and pathological characteristics. Material and methods: For this study we analyzed samples of transrectal prostate biopsies from tumors with different characteristics, treated with or whithout androgen deprivation (AD). After optimization of the culture method, fibroblasts were isolated and cultured to perform the study (PCR) of MMP11 mRNA. Results: Finally, 37 cases were studied: 5 samples of benign prostatic hyperplasia, 14 cases with localized neoplasms (7 high-risk according to the D’Amico classification), 5 with metastasic tumors (bone metastases), and 13 treated with AD therapy, of which 6 fulfilled the requirements to be defined as resistant to castration. In tumors without AD therapy, MMP11 expression was significantly higher (P= .001) in fibroblasts of higher grade tumors. A significant (P= .001) correlation was found between PSA and expression of MMP11 in fibroblast s and a significant increase of MMP11 expression in metastatic tumors. In tumors with AD therapy, a significantly greater expression of MMP11 was observed in resistant to castration patients than in those sensitive to castration (P= .003). Conclusion: In advanced prostate tumors or in stages of increased tumor aggressiveness, the production of MMP11 by fibroblasts is significantly greater than in non-metastatic tumors or in AD sensitive tumors


Assuntos
Humanos , Masculino , Neoplasias da Próstata/patologia , Metaloproteinase 11 da Matriz/análise , Neoplasias de Próstata Resistentes à Castração/patologia , Biomarcadores Tumorais/análise , Estadiamento de Neoplasias , Fibroblastos/ultraestrutura , Células Estromais/patologia
15.
Arch. esp. urol. (Ed. impr.) ; 70(6): 617-620, jul.-ago. 2017. ilus
Artigo em Espanhol | IBECS | ID: ibc-164566

RESUMO

OBJETIVO: Describir y conocer los tumores de células de la granulosa testicular del adulto (TCG) (clasificado como tumores del estroma y cordones sexuales de las gónadas) ya que conforman una variante rara con pocos casos publicados y de comportamiento clínico poco conocido. Método; Presentación de un nuevo caso de TCG testicular del adulto en un varón de 59 años, asintomático, con hallazgo ecográfico de una masa intratesticular de 3,3 cm heterogénea, con áreas sólidas y quísticas; marcadores tumorales y estudio de extensión negativos. RESULTADO: Confirmación del caso con análisis anatomopatológico e inmunohistoquímico, similar a su homólogo ovárico. CONCLUSIONES: El TCG del adulto, es un tumor infrecuente de comportamiento incierto. Aunque suele cursar con buen pronóstico, se ha descrito su potencial metastásico incluso años después de la orquiectomía, por lo que requiere un seguimiento a largo plazo


OBJECTIVE: To describe the adult type granulosa cell testicular tumors (classified as sex cord-stromal tumor) due to their behavior, hardly known with a small number of cases reported. METHOD: We report a new case of a 59-year-old man presenting an adult type granulosa cell tumor of the testis (AGCTT), painless, with a 3.3 centimeter intratesticular heterogeneous mass on ultrasound, with solid and cystic areas. Serum tumor markers and extension study were negative. RESULTS: Histologic and inmunohistochemical studies confirmed an AGCTT, similar to its ovarian counterpart. CONCLUSION: AGCTT are rare neoplasms with unpredictable behavior. Their metastatic potential has been described, reason why they need a long follow-up; however, they usually have a good prognosis


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Tumor de Células da Granulosa/patologia , Neoplasias Testiculares/patologia , Orquiectomia , Células Estromais/patologia , Cordão Espermático/patologia
16.
Eur. j. anat ; 21(2): 97-112, abr. 2017. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-163135

RESUMO

The gastrointestinal stromal tumour (GISTs), the most common mesenchymal neoplasm in the gastrointestinal tract, has been the subject of great interest in recent years in terms of prognosis, diagnosis and treatment. Its etiology is linked to the mutation of c-KIT and PDGFRA genes, although between 5 and 15% show no signs of such mutations. It is still diagnosed using immunohistochemical staining. The first line of treatment continues to be surgery, although advances in the molecular biology of GISTs are facilitating the development of new treatment strategies. Those that act by regulating tyrosine kinase activity are of particular interest. Drugs such as imatinib and sunitinib have improved the prognosis of these patients, although the development of resistance constitutes one of the main limitations of the treatment. The aim of this review is to present an up-to-date overview of the main etiopathogenic, diagnostic and therapeutic aspects of these tumours


No disponible


Assuntos
Humanos , Tumores do Estroma Gastrointestinal/patologia , Mesoderma/patologia , Neoplasias Gastrointestinais/patologia , Células Estromais/patologia , Nanotecnologia/métodos , Proteínas Proto-Oncogênicas c-kit/análise , Marcadores Genéticos
17.
J. physiol. biochem ; 73(1): 1-4, feb. 2017. ilus
Artigo em Inglês | IBECS | ID: ibc-168387

RESUMO

Obesity is a leading health problem facing the modern world; however, no effective therapy for this health issue has yet been developed. A promising research direction to identify novel therapies to prevent obesity has emerged from discoveries on development and function of brown/brite adipocytes in mammals. Importantly, there is evidence for the presence and function of active thermogenic brown adipocytes in both infants and adult humans. Several new investigations have shown that thermogenic adipocytes are beneficial to maintain glucose homeostasis, insulin sensitivity, and a healthy body fat content. Such thermogenic adipocytes have been considered as targets to develop a therapy for preventing obesity. This short review seeks to highlight recent findings on the development and function of brown/brite adipocytes in humans and to discuss potential treatments based on these adipocytes to reduce obesity and its related disorders (AU)


No disponible


Assuntos
Humanos , Animais , Adipócitos Bege/metabolismo , Adipócitos Marrons/metabolismo , Adipogenia , Modelos Biológicos , Termogênese , Adipócitos Brancos , Adiposidade , Capilares/citologia , Resistência à Insulina , Obesidade , Células Estromais/citologia
19.
Rev. esp. patol ; 49(1): 62-65, ene.-mar. 2016. ilus
Artigo em Espanhol | IBECS | ID: ibc-149069

RESUMO

Los tumores de células de la granulosa (TCG) pertenecen al grupo de tumores del estroma y cordones sexuales de las gónadas. Se distinguen 2 tipos: juvenil y adulto. Los TCG de tipo adulto testicular son extremadamente raros, y solo un pequeño número de casos han sido publicados. Su comportamiento biológico es incierto, con casos descritos de metástasis a distancia años después de su presentación inicial. Su diagnóstico se basa en el reconocimiento de las características citológicas y arquitecturales descritas en su homólogo ovárico, ya que ambos son morfológicamente indistinguibles. Describimos un nuevo caso de TCG de tipo adulto testicular en un varón de 23 años, haciendo hincapié en su comportamiento clínico, así como en las características inmunofenotípicas de este raro tumor (AU)


Granulosa cell tumors (GCT) belong to the group of sex cord-gonadal stromal tumours. Two types can be differentiated: juvenile and adult. Adult testicular GCT are extremely rare with only a small number of published cases. Their biological behaviour is not completely clear with cases of distant metastasis appearing years after the initial diagnosis. Diagnosis is based on the cytological and architectural characteristics described in its ovarian counterpart, since both are morphologically indistinguishable. We present a further case of GCT of the adult type in a 23 year old man. We discuss the clinical behavior and immunophenotypical characteristics of this rare tumour (AU)


Assuntos
Humanos , Masculino , Adulto , Tumor de Células da Granulosa/complicações , Tumor de Células da Granulosa/diagnóstico , Tumor de Células da Granulosa/patologia , Neoplasias Testiculares/patologia , Células Estromais , Tumores do Estroma Gonadal e dos Cordões Sexuais/patologia , Neoplasias Testiculares , Testículo/patologia , Testículo
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