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1.
Allergol. immunopatol ; 52(1): 60-64, 01 jan. 2024. tab, graf
Artigo em Inglês | IBECS | ID: ibc-229179

RESUMO

Purpose: The importance of carbohydrates in anaphylaxis has been described with some foods. The current work intends to obtain clinical and immunological evidence of the importance of the O-glycans for IgE binding activity in anaphylactic reactions due to Helix aspersa (HA) ingestión and Artemisia vulgaris (AV) exposition. Methods: The studio focused on two cases of IgE-mediated anaphylaxis induced by snail ingestion in patients with underlying rhino-conjunctivitis and asthma due to AV. We performed on both patients: skin prick tests ( SPTs) with HA and AV and with a battery of aeroallergen, controlled nasal challenge and specific IgE to HA and AV, ImmunoCAP ISAC®, and a differential pattern of IgE recognition with SDS-PAGE Immunoblotting (SDSI) when these allergens have suffered an O-deglycosylation procedure. Results: The patients showed positive results in SPTs, nasal challenges, and serum-specific IgE against HA and AV. In patient 1, the SDSI detected several IgE-binding proteins in AV with a molecular mass of 22, 24, and 44 kDa, whereas a band of 12 kDa was detected in HA. On the other hand, patient 2’s serum revealed an IgE-binding zone between 75 and 20 kDa in the AV and a band of 24 kDa in the HA. When glycans were removed, patient 1’s serum only revealed the AV’s 22 and 24 kDa bands, whereas patient 2’s serum did not detect any IgE-reactive protein in the HA. Conclusions: Our data suggest that O-glycosylation can be relevant in patients with anaphylaxis due to snails and allergy to Artemisia vulgaris. This new entity representing cross-reactivity between AV and HA could be named Snail-Artemisia Syndrome (AU)


Assuntos
Humanos , Masculino , Feminino , Adulto , Anafilaxia/diagnóstico , Anafilaxia/etiologia , Artemisia , Caramujos , Carboidratos , Imunoglobulina E , Polissacarídeos
2.
Int. microbiol ; 26(4): 961-972, Nov. 2023. graf
Artigo em Inglês | IBECS | ID: ibc-227484

RESUMO

Phytases are specialized enzymes meant for phytic acid degradation. They possess ability to prevent phytic acid indigestion, including its attendant environmental pollution. This study was aimed at investigating biochemical properties of purified phytase of B. cereus isolated from Achatina fulica. Phytase produced from Bacillus cereus that exhibited optimal phytate degrading-ability of all the bacteria isolated was purified in a three-step purification. The biochemical properties of the purified enzyme were also determined. The phytase homogeny of approximately 45 kDa exhibited 12.8-purification fold and 1.6% yield with optima phytate degrading efficiency and maximum stability at pH 7 and 50 °C. Remaining activity of 52 and 47% obtained between 60 and 70 °C after 2 h further established thermostability of the purified phytase. Mg2+ and Zn2+ enhanced phytate hydrolysis by the enzyme, while Na+ showed mild inhibition but Hg2+ severely inhibited the enzymatic activity. Km and Vmax were estimated to be 0.11 mM and 55.6 μmol/min/mL, displaying enzyme-high substrate affinity and catalytic efficiency, respectively. Phytase purified from Bacillus cereus, isolated from African giant snails, has shown excellent characteristics suitable for phytic acid hydrolysis and could be employed in industrial and biotechnological applications.(AU)


Assuntos
Humanos , Ácido Fítico/química , 6-Fitase/química , Trato Gastrointestinal , Bacillus cereus/metabolismo , Caramujos/metabolismo , Prótons , 6-Fitase/metabolismo , Microbiologia , Técnicas Microbiológicas , Ácido Fítico/metabolismo
3.
Rev. toxicol ; 37(1): 19-25, 2020. ilus, tab, graf
Artigo em Inglês | IBECS | ID: ibc-194441

RESUMO

Uncontrolled waste disposal has continuously threatened the health of the surrounding environment through the leaching of hazardous xenobiotics. Systemic toxicity and genotoxicity potential of waste leachates from Onitsha municipal dumpsite were investigated in giant African land snail (Limicolaria aurora) through oxidative stress biomarker and micronucleus test assessment respectively. Physicochemical indices were evaluated in the leachate following standard protocols. Snails were exposed to different concentrations (0, 6.25, 12.5, 25.0 and 50.0%) of waste leachate for 21 days; oxidative stress biomarkers and micronucleus analysis performed on snail digestive gland and hemocyte respectively. The leachate induced dose-duration dependent increase (P< 0.05) in Superoxide dismutase, Catalase, malondialdehyde and Glutathione peroxidase levels with associated decrease in total protein concentrations in the exposed snails compared to the control. Similarly, the frequency of micronucleus and other nuclear abnormalities shows concentration dependent increase (P< 0.05) in treated groups. This observed genotoxic effect might be induced by the oxidative stress, via the production of reactive oxygen species. This shows that waste leachate contains hazardous and genotoxic compounds capable of inducing oxidative stress and DNA damage. Therefore, continuous exposure of waste leachate into the environment could pose a grave health risk to the surrounding biota, humans included


No disponible


Assuntos
Animais , Estresse Oxidativo/genética , Esgotos , Eliminação de Resíduos Líquidos , Caramujos/genética , Micronúcleo Germinativo/genética , Monitoramento Ambiental/métodos , 34709
4.
Rev. toxicol ; 34(2): 118-123, jul.-dic. 2017. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-169821

RESUMO

Los productos farmacéuticos son ampliamente utilizados en todo el planeta. Existe una creciente preocupación por los efectos que los medicamentos consumidos y los desechos de estos producen en el ambiente. A pesar del gran uso de los antibacterianos no son muy investigados como contaminantes, prestándose mayor atención a la antibioresistencia por lo que el objetivo de la investigación fue evaluar la ecotoxicidad aguda de antibacterianos. Teniendo en cuenta el consumo de los antibacterianos y la predicción de sus concentraciones ambientales así como su ecoxicidad en Lactuca sativa L ya previamente determinados en anteriores investigaciones se determinó el riesgo ecotoxicológico en Artemia salina L. y Physa cubensis P. En el ensayo de Artemia salina la Ceftazidima se clasifica como muy tóxico con valor de CL50 de 0,060773 g/L, la Cefepima con valor de 0,993731 g/L como moderadamente tóxica y el resto de los antibacterianos evaluados se clasifican como no tóxicos. En los bioensayos en Physa cubensis Cefepima y Cefazolina ocasionaron la mayor mortalidad con CL50 de 0,000270 y 0,025684 g/L respectivamente y los que indujeron menor mortalidad fueron Vancomicina y Amoxicilina/Sulbactam con CL50 de 1,528440 y 1,055492 g/L. El vertimiento de residuos de antibacterianos puede ser causa de contaminación ambiental perjudicial para algunas especies (AU)


Pharmaceuticals are widely used all over the planet. There is growing concern about the effects that drugs and its residues produce in the environment. Despite the wide use of antibacterial, they are not very investigated as pollutants, paying greater attention to the antibiotic resistance, so the objective of this research was to evaluate the antibacterial acute ecotoxicity. Taking into account the antibacterial consumption and the prediction of their environmental concentrations as well as their ecotoxicity in Lactuca sativa L already determined in previous research, the ecotoxicological risk was determined in Artemia salina L. and Physa cubensis P. In the Artemia salina trial Ceftazidime is classified as very toxic with LC50 value of 0.060773g/L, Cefepime with value of 0.993731g/L is classified as moderately toxic and the rest of antibacterial evaluated are classified as non-toxic. In bio trials of Physa cubensis, Cefepime and Cefazolin caused the greatest mortality with LC50 0.000270 and 0.025684g/L respectively, and the ones that led lower mortality were Vancomycin and Amoxicillin/Sulbactam with LC50 of 1.528440 and 1.055492 g/L. The dumping of antibiotics residues can be the cause of environmental pollution, detrimental to some species (AU)


Assuntos
Ecotoxicologia/métodos , Antibacterianos/isolamento & purificação , Artemia , Caramujos , Poluição da Água/análise , Substâncias Perigosas/isolamento & purificação , Riscos Ambientais
5.
Eur. j. anat ; 15(1): 10-16, ene. 2011. ilus
Artigo em Inglês | IBECS | ID: ibc-108074

RESUMO

The physiological functions of the embryonic epicardium have been neglected for decades. However, we currently know that this tissue is an essential element in cardiac development. In this review we summarize the available knowledge about how the embryonic epicardium arises from an extracardiac progenitor tissue, the proepicardium, a cluster of coelomic cells that adhere to and spread over the myocardial surface. We describe the control, at molecular level, of the epicardial epithelialmesenchymal transition leading to the formation of the epicardial-derived cells (EPDC), which are highly invasive mesenchymal cells that invade the subepicardial space and the myocardium. We briefly discuss the developmental fate of the EPDC and their contribution to the development of the coronary vessels. Furthermore, epicardial cells and EPDC interact with the developing ventricular myocardium, promoting its maturation and growth, through a poorly-known mechanism that seems to be dependent on retinoic acid signaling. We finish this review with a discussion of the evolutionary origin of the proepicardium and epicardium, and their relationship with the primitive excretory system of vertebrates. This relationship can account for some of the peculiar features of epicardial development (AU)


No disponible


Assuntos
Humanos , Animais , Pericárdio/embriologia , Coração/embriologia , Vasos Coronários/embriologia , Genes do Tumor de Wilms , Caderinas , Caramujos/embriologia
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