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1.
Int. microbiol ; 26(4): 1143-1155, Nov. 2023. ilus
Artigo em Inglês | IBECS | ID: ibc-227499

RESUMO

One of the biggest global causes of death is cancer. The side effects of currently available therapies have triggered the search for new drugs. The marine environment, with its vast biodiversity, including sponges, is a rich source of natural products with immense pharmaceutical potential. The aim of the study was to analyze the microbes associated with the marine sponge, Lamellodysidea herbacea, and explore them as resources for anticancer ability. This study includes the isolation of fungi from L. herbacea, and their evaluation for cytotoxic potential against human cancer cell lines such as A-549 (lung), HCT-116 (colorectal carcinoma), HT-1080 (Fibrosarcoma), and PC-3 (prostate) using MTT assay. This revealed that fifteen extracts showed significant anticancer ability (IC50 ≤ 20 µg/mL), at least against one of the cell lines. Three extracts, SPG12, SPG19, and SDHY 01/02, were found significant in terms of anticancer activity, at least against three to four cell lines (IC50 values ≤ 20 µg/mL). The fungus SDHY01/02 was identified by sequencing the internal transcribed spacer (ITS) region as Alternaria alternata. Its extract showed IC50 values < 10 µg/mL against all the tested cell lines and was further analysed through light and fluorescence microscopy. The extract of SDHY01/02 was active (lowest IC50 4.27 µg/mL) against A549 cells in a dose-dependent manner and caused apoptotic cell death. Further, the extract was fractionated and analyzed the constituents by GC-MS (Gas Chromatography-Mass Spectrometry). Di-ethyl ether fraction revealed the constituents (having anticancer activity) such pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(2-methyl propyl); 4,5,6,7-tetrahydro-benzo[C]rhiophene-1-carboxylic acid cyclopropylamide; 17-pentatriacontene; 9,12-octadecadienoic acid (Z, Z)-, methyl ester; while DCM fraction contained Oleic acid, eicosyl ester. This is the first report of A. alternata with anticancer potential that has been isolated from the sponge L. herbacea, as far as we are aware.This A. alternata can be exploited to get anticancer molecule(s) in the future.(AU)


Assuntos
Humanos , Masculino , Feminino , Alternaria , Ésteres , Linhagem Celular Tumoral , Extratos Vegetais/química , Poríferos , Microbiologia , Técnicas Microbiológicas , Neoplasias , Filogenia
2.
Rev. esp. quimioter ; 21(3): 174-179, sept. 2008. tab
Artigo em Espanhol | IBECS | ID: ibc-77588

RESUMO

Como parte de la búsqueda de sustancias activas antimicrobianasnovedosas se analizó la actividad in vitro de losextractos orgánicos crudos de 15 especies de esponjas marinasde la costa noreste de Colombia contra microorganismosde importancia clínica en humanos (una cepa de cada especiede Streptococcus faecalis, Staphylococcus aureus, Escherichiacoli, Pseudomonas aeruginosa y Candida albicans).Los extractos de las esponjas Halichondria sp., Petromicaciocalyptoides y Xestospongia proxima exhibieron actividadcontra las bacterias grampositivas y contra el hongo, mientrasque la esponja Dragmacidon reticulata sólo mostró actividadcontra esta levadura. La bioactividad de los extractosfue contrastada con un antibiótico (cefoperazona) y un antimicótico(nistatina) comerciales y se encontró que los valoresde inhibición in vitro del extracto de X. proxima son mayores,en algunos casos, a los observados para la cefoperazona yla nistatina. Los extractos de las esponjas Myrmekiodermagyroderma, Myrmekioderma rea, Biemna cribaria, Cinachyrellakuekenthali, Iotrochota imminuta, Oceanapia peltata,Polymastia tenax, Desmapsamma anchorata, Spirastrellacoccinea, Cribrochalina infundibulum y Oceanapia bartschino presentaron actividad antimicrobiana (AU)


As part of the search for new natural sources of antibioticcompounds, in this study, carried out in the northeasterncoast of Colombia, 15 sponge species were collected.A crude organic extract was obtained from each one and evaluated regarding their antimicrobial propertiesin vitro against microorganisms with clinical importancefor humans (one strain for each specie of Streptococcusfaecalis, Staphylococcus aureus, Escherichia coli, Pseudomonasaeruginosa and Candida albicans). Sponge extractsfrom Halichondria spp., Petromica ciocalyptoides and Xestospongiaproxima exhibited antibacterial activity againstgram-positive bacteria and antifungal activity against thefungi, while the sponge Dragmacidon reticulata showedactivity only for the same yeast specie. Bioactivity of theextracts was compared with that of both a antibiotic (cefoperazone)and an antimicotic (nistatine). It was found thatinhibition values of X. proxima extracts in vitro are, in somecases, higher than those observed for cefoperazone andnistatine. Crude extracts from the sponges Myrmekiodermagyroderma, Myrmekioderma rea, Biemna cribaria, Cinachyrellakuekenthali, Iotrochota imminuta, Oceanapiapeltata, Polymastia tenax, Desmapsamma anchorata, Spirastrellacoccinea, Cribrochalina infundibulum and Oceanapiabartschi did not show any antimicrobial activitywhatsoever (AU)


Assuntos
Animais , Poríferos/anatomia & histologia , Poríferos/classificação , Poríferos/enzimologia , Poríferos/crescimento & desenvolvimento , Poríferos/microbiologia , Flora Marinha/análise , Flora Marinha/história , Ambiente Marinho/análise , Ambiente Marinho/métodos , Bactérias , Fungos
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