RESUMO
Objective: To assess the therapeutic effect and mechanism of 6'-o-galloylpaeoniflorin (GPF) in pediatric pneumonia.Methods: The effects of lipopolysaccharide (LPS) and GPF on cell viability and apoptosis were examined by cell counting kit-8 assay and flow cytometry analysis. The oxidative stress and inflammatory response were assessed by detecting expression levels of superoxide dismutase, glutathione, r-glutamyl cysteingl+glycine, myeloperoxidase, and malondialdehyde as well as tumor necrosis factor-α, Interleukin-18, and Interleukin-10 by using enzyme-linked-immuno-sorbent serologic assay. Moreover, the activation of nuclear factor erythroid 2-related factor 2 (Nrf2) pathway was detected by immunoblot assay, and the influence of Nrf2-knockdown on cell viability, oxidative stress, and inflammation response was also investigated.Results: The results established that GPF increased the viability of LPS-induced pneumonia cells. In addition, GPF reduced LPS-induced oxidative stress in pneumonia cells. It was further discovered that GPF reduced LPS-induced inflammation in pneumonic cell. GPF improved the activity of Nrf2 in LPS-treated pneumonic cells, and therefore alleviated inflammation and oxidative stress in pediatric pneumonia.Conclusion: GPF could serve as a promising drug for treating pediatric pneumonia (AU)
Assuntos
Humanos , Criança , Lipopolissacarídeos , Fator de Transcrição NF-E2 , Pneumonia/tratamento farmacológico , Compostos Bicíclicos Heterocíclicos com Pontes/metabolismo , Glucosídeos/metabolismo , Inflamação/tratamento farmacológico , Lipopolissacarídeos/metabolismo , Lipopolissacarídeos/farmacologia , Lipopolissacarídeos/uso terapêutico , Monoterpenos , Fator de Transcrição NF-E2/metabolismo , Fator de Transcrição NF-E2/farmacologia , Fator de Transcrição NF-E2/uso terapêutico , Estresse Oxidativo , Transdução de SinaisRESUMO
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