RESUMO
No disponible
Assuntos
Humanos , Bezoares/tratamento farmacológico , Celulase/uso terapêutico , Pancreatina/uso terapêutico , Ácido Ursodesoxicólico/uso terapêutico , Bebidas Gaseificadas , Resultado do TratamentoRESUMO
No disponible
Assuntos
Humanos , Pimenta/efeitos adversos , Capsicum/efeitos adversos , Hipersensibilidade Alimentar/diagnóstico , Anafilaxia/diagnóstico , Esofagite Eosinofílica/diagnóstico , Reações Cruzadas/imunologia , Immunoblotting/métodos , Celulase/efeitos adversos , Peroxidase/efeitos adversos , Alérgenos/isolamento & purificaçãoRESUMO
No disponible
Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Obstrução Intestinal/etiologia , Bezoares/complicações , Acloridria/complicações , Motilidade Gastrointestinal , Fatores de Risco , Celulase/uso terapêuticoRESUMO
Fungal strains naturally occurring on the wood and leaves of the salt-excreting desert tree Tamarix were isolated and characterized for their ability to produce cellulose- and starch- degrading enzymes. Of the 100 isolates, six fungal species were identified by ITS1 sequence analysis. No significant differences were observed among taxa isolated from wood samples of different Tamarix species, while highly salt-tolerant forms related to the genus Scopulariopsis (an anamorphic ascomycete) occurred only on the phylloplane of T. aphylla. All strains had cellulase and amylase activities, but the production of these enzymes was highest in strain D, a Schizophyllum-commune- related form. This strain, when grown on pretreated Tamarix biomass, produced an enzymatic complex containing levels of filter paperase (414 ± 16 IU/ml) that were higher than those of other S. commune strains. The enzyme complex was used to hydrolyze different lignocellulosic substrates, resulting in a saccharification rate of pretreated milk thistle (73.5 ± 1.2 %) that was only 10 % lower than that obtained with commercial cellulases. Our results support the use of Tamarix biomass as a useful source of cellulolytic and amylolytic fungi and as a good feedstock for the economical production of commercially relevant cellulases and amylases (AU)
No disponible
Assuntos
Glicosídeo Hidrolases/isolamento & purificação , Fungos/crescimento & desenvolvimento , Plantas/microbiologia , Schizophyllum/crescimento & desenvolvimento , Tamaricaceae/microbiologia , Celulase/isolamento & purificação , Amilases/isolamento & purificação , BiomassaRESUMO
The ability of earthworms to decompose lignocellulose involves the assistance of microorganisms in their digestive system. While many studies have revealed a diverse microbiota in the earthworm gut, including aerobic and anaerobic microorganisms, it remains unclear which of these species contribute to lignocellulose digestion. In this study, aerobic microorganisms with cellulolytic activity isolated from the gut of two endogeic earthworms, Amynthas heteropoda (Megascolecidae) and Eisenia fetida (Lumbricidae) were isolated by solid culture of gut homogenates using filter paper as a carbon source. A total of 48 strains, including four bacterial and four fungal genera, were isolated from two earthworm species. Characterization of these strains using enzyme assays showed that the most representative ones had exocellulase and xylanase activities, while some had weak laccase activity. These findings suggest that earthworms digest lignocellulose by exploiting microbial exocellulase and xylanase besides their own endocellulase. Phylogenetic analysis showed that among the cellulolytic isolates in both earthworm species Burkholderia and Chaetomium were the dominant bacterial and fungal members (AU)
No disponible
Assuntos
Animais , Bactérias Aeróbias/isolamento & purificação , Bactérias Aeróbias/metabolismo , Fungos/isolamento & purificação , Fungos/metabolismo , Lignina/metabolismo , Oligoquetos/microbiologia , Xilosidases/metabolismo , Bactérias Aeróbias/enzimologia , Bactérias Aeróbias/genética , Celulase/metabolismo , Fungos/enzimologia , Fungos/genética , Trato Gastrointestinal/microbiologia , FilogeniaRESUMO
No disponible
Assuntos
Humanos , Bezoares/tratamento farmacológico , Celulase/uso terapêutico , Fibras na Dieta/efeitos adversosRESUMO
El autor presenta la necesidad de protocolizar los métodos de diagnóstico y tratamiento con el fin de sistematizar la terapia antienvejecimiento. En relación a los protocolos establecidos, remarca la importancia de valorar, de forma práctica, las distintas pruebas tanto químicas como biológicas: estudio de los componentes sanguíneos, de la composición corporal, el índice de masa corporal o BMl, el índice metabólico basal o BMR, los análisis del colesterol, lipoproteínas, triglicéridos y glucosa y el llamado estado biológico. A continuación expone como Se pueden valorar todos estos datos obtenidos para poder aplicar el correspondiente tratamiento mediante un aporte enzimático (enzimas digestivas, metabólicas y alimentarias), analizando la influencia corporal de los déficits de determinadas enzimas alimentarias (proteasa, amilasa, lipasa, celulasa, etc) así como sus mecanismos y niveles de actuación para finalmente exponer una serie de puntos clave y las diferentes soluciones recomendables para modificar el proceso del envejecimiento con especial énfasis en el ejercicio y sus diversas modalidades (AU)
