Investigation of serum IL-12, IL-16, and IL-17A as diagnostic biomarkers in children with cow’s milk protein allergy

Background Cow’s milk protein allergy (CMPA) is an abnormal immune response caused by milk proteins and is most common in infancy and early childhood. Statistics revealed up to 7.5% of children suffered from milk allergy. Its clinical symptoms were characterized by diversity, non-specificity, and can affect multiple systems, including the digestive tract, skin, and respiratory tract. In this study, we aimed to investigate the effects of IL-12, IL-16, and IL-17A on diagnosing and monitoring CMPA in children for clinical treatment.Methods A total of 158 infants with CMPA and 89 healthy babies were recruited and evaluated. Demographic and clinical information of all participants were recorded. An extensive analysis of inflammatory cytokine levels, including IL-12, IL-16, and IL-17A, was performed in blood samples from 247 infants younger than 9 months. Meanwhile, the serological specificity immunoglobulin E (sIgE) levels were evaluated. In addition, the area under the curve (AUC) values of IL-12, IL-16, and IL-17A in differentiating CMP from healthy babies were measured by receiver operating characteristic analysis. Finally, the correlation between sIgE and IL-12, IL-16, and IL-17A levels were detected using Spearman correlation analysis.Results Compared with healthy control, infants who developed CMPA had decreased IL-12, increased IL-16, and IL-17A. Moreover, a significant correlation between serum IL-12, IL-16, IL-17A and sIgE levels was observed in the CMPA group. In addition, AUC values of IL-12, IL-16, and IL-17A in discriminating CMPA from healthy infants were 0.8425, 0.9196, and 0.8813, respectively. Finally, IL-12 was increased while IL-16 and IL-17A levels were decreased in the CMPA group after three months of milk avoidance treatment.Conclusions We found that IL-12, IL-16, and IL-17A levels in children with CMPA were associated with SCORAD scores, sIgE levels (AU)

Concentraciones de interleucina-16 en pacientes con preeclampsia y gestantes normotensas sanas / Interleukin-16 Concentrations In Patients With Preeclampsia And Healthy Normotensive Pregnant Patients

Objetivo Comparar las concentraciones de interleucina-16 en pacientes con preeclampsia y gestantes normotensas sanas. Método Se seleccionó un total de 100 pacientes. Se incluyeron 50 pacientes con preeclampsia como grupo de estudio (grupo A) y un grupo de control seleccionado por tener una edad y un índice de masa corporal similares al grupo de estudio que consistió en 50 gestantes normotensas sanas (grupo B). Las muestras de sangre se recolectaron en todas las pacientes antes del parto e inmediatamente después del diagnóstico en el grupo B para determinar las concentraciones de interleucina-16.ResultadosSe encontraron diferencias estadísticamente significativas en las concentraciones de interleucina-16 entre las pacientes del grupo de estudio (grupo A: 211,9 ± 78,7 pg/ml) y las pacientes del grupo de control (grupo B: 83,6 ± 9,9 pg/ml; p < 0,05). Se observó una correlación fuerte, positiva y significativa con los valores de presión arterial sistólica (r = 0,282; p < 0,05) y con los valores de presión arterial diastólica (r = 0,320; p < 0,05). Un valor de corte de 180 pg/ml presentó un valor por debajo de la curva de 0,95, una sensibilidad del 94,0%, especificidad del 70,0%, valor predictivo positivo del 75,8% y valor predictivo negativo del 92,1%, con una exactitud diagnóstica del 75,0%.ConclusionesLas pacientes con preeclampsia presentaron concentraciones significativamente más altas de interleucina-16 al compararlo con gestantes normotensas sanas (AU)

Objective To compare interleukin-16 concentrations in patients with preeclampsia and healthy normotensive pregnant women. Method A total of 100 patients were selected. Fifty patients with preeclampsia were selected as the study group (group A) and 50 healthy normotensive pregnant women with the same age and body mass index as the study group were selected as controls (group B). Blood samples were extracted from all patients before labor and immediately after diagnosis in group B to determine interleukin-16 concentrations. Results There was statistically significant difference in interleukin-16 concentrations between group A (211.9 ± 78.7 pg/ml) and group B (83.6 ± 9.9 pg/ml; p < 0.05). There was a strong, positive and significant correlation with systolic blood pressure values (r = 0.282; p < 0.05) and with diastolic blood pressure values (r = 0.320; p < 0.05). A cutoff value of 180 pg/ml had an area under the curve of 0.95, sensitivity of 94.0%, specificity of 70.0%, a positive predictive value of 75.8% and a negative predictive value of 92.1%, with a diagnostic accuracy of 75.0%.ConclusionsInterleukin-16 concentrations were significantly higher in patients with preeclampsia than in healthy normotensive pregnant women (AU)