Effect of cell density on the malignant biological behavior of breast cancer by altering the subcellular localization of ANXA2 and its clinical implications

To investigate the subcellular localization of ANXA2 in breast cancer of different cell densities in humans and its relationship with the clinicopathological features of patients. To investigate the differences in ANXA2 subcellular localization in MDA-MB-231 cells of different cell densities. To compare the proliferation, invasion, and migration ability of MDA-MB-231 cells under different ANXA2 subcellular localization.MethodsImmunohistochemistry was applied to detect the subcellular localization of ANXA2 in tissue sections of 60 breast cancer patients, and the association with ANXA2 subcellular localization was verified in conjunction with cell density. To investigate the relationship between cell density and clinicopathological data of breast cancer patients. To establish high- and low-density models of MDA-MB-231 breast cancer cell lines and verify the subcellular localization of ANXA2 using immunofluorescence and observation under confocal microscopy. The proliferation, migration, and invasion ability of MDA-MB-231 cells under different subcellular localization of ANXA2 were detected and compared using CCK-8 assay and Transwell assay. After changing the subcellular localization of ANXA2 in high-density MDA-MB-231 cells with PY-60, changes in biological behaviors of the compared MDA-MB-231 cells were observed. Two different 4T1 cell lines with high and low densities were implanted subcutaneously in nude mice to observe the effects of different cell densities on tumor growth in nude mice.ResultsThe clinical data showed that breast cancer with high cell density had higher T stage and higher TNM stage, and the cell density was positively correlated with breast cancer mass size. ANXA2 was mainly localized to the cell membrane when the cell density of breast cancer cells was high and to the cytoplasm when the cell density was low. (AU)

Expresión de la anexina A2 en los carcinomas epidermoides de cabeza y cuello / Annexin A2 expression in head and neck squamous cell carcinoma

Objetivo: La expresión de la anexina A2 (ANXA2) se ha hallado elevada en varios cánceres. Sin embargo, no hay datos disponibles de la expresión de esta proteína en los carcinomas epidermoides de cabeza y cuello. El objetivo de este estudio preliminar es investigar la expresión de la ANXA2 en estos carcinomas. Material y método: Se analizó la expresión de la ANXA2 mediante inmunohistoquímica en muestras incluidas en parafina de 9 lesiones premalignas y 21 carcinomas epidermoides de cabeza y cuello. Resultados: Todas las lesiones con displasia mostraron una reducción en la expresión de la ANXA2 respecto al tejido normal. En contraste, se apreció expresión de la ANXA2 en todos menos uno de los tumores estudiados. La disminución de la expresión de la ANXA2 en los carcinomas se correlacionó de forma significativa con una peor diferenciación histológica, con tumores de mayor tamaño y con metástasis ganglionares. Conclusiones: Nuestros datos muestran por primera vez que la ANXA2 se expresa en los carcinomas epidermoides de cabeza y cuello e indican que su expresión se relaciona con el grado de diferenciación de estos tumores

Objective: Over-expression of annexin A2 (ANXA2) has been reported in various cancers. However, no data are available on the expression of this protein in head and neck squamous cell carcinomas (HNSCC). The objective of this preliminary study is to investigate the expression of ANXA2 in these carcinomas. Material and method: ANXA2 expression was analyzed by immunohistochemistry in paraffin-embedded sections from 9 patients with premalignant lesions and 21 patients with HNSCC. Results: All dysplastic tissues showed significantly reduced ANXA2 expression compared to normal tissue. In contrast, ANXA2 expression was observed in all but one of the tumours studied. There was a significant correlation of lower ANXA2 expression with a poorer histological differentiation, larger tumours, and nodal metastases. Conclusions: Our data show for the first time that ANXA2 is expressed in head and neck squamous cell carcinomas and that its expression seems to be related with the degree of differentiation status of these tumours

Expresión de las anexinas A1 y A2 en la mucosa del tracto aerodigestivo superior / Expression of annexins A1 and A2 in the mucosa of the upper aerodigestive tract

Introducción: Las anexinas A1 y A2 se han relacionado con el mantenimiento de la integridad tisular. Han sido identificadas en varios tejidos humanos pero su expresión en el tracto aerodigestivo superior es poco conocida. El objetivo de este trabajo es estudiar la expresión de estas proteínas en la mucosa del tracto aerodigestivo superior. Material y métodos: Se estudian muestras de mucosa respiratoria (nasal y laríngea) y digestiva (de cavidad oral y faringe) de pacientes intervenidos de cirugía no oncológica. La expresión de las anexinas A1 y A2 se estudia por inmunohistoquímica. Resultados: Ambas anexinas se expresan tanto en el epitelio ciliado pseudoestratificado como en el plano poliestratificado no queratinizado, pero con un patrón diferente; la anexina A1 se expresa en las células más diferenciadas, mientras que la A2 lo hace en las menos diferenciadas (con la excepción de los cilios de las células ciliadas). Conclusión: Aunque las anexinas A1 y A2 están estructural y filogenéticamente relacionadas, su diferente expresión en la mucosa del tracto aerodigestivo superior sugiere que participan en funciones claramente diferenciadas (AU)

INTRODUCTION: Annexins A1 and A2 have been related with the maintenance of tissue integrity. They have been identified in a wide variety of tissues, but little is known regarding their expression in upper the aerodigestive tract. The aim of this work is to describe the expression of these proteins in the mucosa of the upper aerodigestive tract. MATERIAL AND METHODS: Tissue samples from respiratory (nasal and laryngeal) and digestive (oral and pharyngeal) mucosa from non-oncological patients were studied. Annexin A1 and A2 expression was determined by immunohistochemistry. RESULTS: Both annexins were expressed in the ciliated and in the stratified non-keratinized epithelia, but with a different pattern; ANXA1 was expressed in the more differentiated cells whereas ANXA2 was expressed in the less differentiated ones (with the exception of the cilia of ciliated cells). CONCLUSION: Although annexins A1 and A2 are structurally and philogenetically related its expression pattern in the upper aerodigestive tract suggests that they have different functions (AU)