Changes and clinical significance of serum MMP-9, TIMP-1, COX-2, and immune levels in patients with asthma

Objective: To detect serum metalloproteinase-9 (MMP-9), tissue inhibitor of metalloproteinases (TIMP-1), cyclooxygenase-2 (COX-2), and T helper cells 1–T helper cells 2 (Th1–Th2) levels in asthma patients and assess their clinical significance. Methods: A total of 72 patients experiencing acute asthma (acute group), 66 stable asthma patients (stable group), and 60 healthy volunteers (control group) were included in this study. The levels of TIMP-1, COX-2, and Th1–Th2 in patients with acute asthma were measured following treatment with budesonide aerosol inhalation. In addition, the levels of MMP-9, TIMP-1, COX-2 and Th1–Th2 were compared in patients with different severity of acute asthma before and after treatment. Results: The serum levels of MMP-9, TIMP-1, and COX-2 showed an increasing trend in the control, stable, and acute groups, while levels of Th1–Th2 showed a sequential decreasing trend, and the differences were statistically significant. Comparison of lung function indexes among the three groups of patients established a negative correlation between serum MMP-9 and its forced vital capacity% predicted (FEV%pred), TIMP-1, and COX-2, and FEV%pred and forced expiratory volume in 1 s–forced vital capacity (FEV1/FVC) levels, but a positive correlation between Th1–Th2 and FEV1/FVC levels in the acute group. A significant difference was observed on comparing the levels of serum MMP-9, TIMP-1, COX-2, and Th1–Th2 in patients with different conditions in the acute group. Specifically, as the condition worsened, a significant increase in serum MMP-9, TIMP-1, and COX-2 levels but a significant decrease in Th1–Th2 levels was observed. After treatment, we observed a significant decrease in serum MMP-9, TIMP-1, and COX-2 levels but a significant increase in Th1–Th2 levels in the acute group(AU)

Correlation analysis of TGF-β1, MMP-9, TIMP-1, IL-1, IL-4, IL-6, IL-17, and TNF-α in refractory chronic rhinosinusitis: A retrospective study

Objective: To investigate the potential correlation of transforming growth factor-β (TGF-β), matrix metalloprotein 9 (MMP-9), tissue inhibitor of metalloproteinases 1 (TIMP-1), Interleukin 1 (IL-1), IL-4, IL-6, IL-17, and tumor necrosis factor alpha (TNF-α) in refractory chronic rhinosinusitis.Methods: A total of 150 participants were retrospectively included in this study from August 2018 to February 2020. The people enrolled were equally allocated into refractory group (patients with refractory chronic rhinosinusitis), chronic group (patients with chronic rhinosi-nusitis), and control group (normal people). The level of TGF-β1, MMP-9, TIMP-1, IL-1, IL-4, IL-6, IL-17, and TNF-α were recorded. The unconditional multivariate binary logistic regression was used to analyze the factors affecting refractory chronic rhinosinusitis.Results: The Davos score, T&T olfactometer threshold test, and Lund-Mackay CT scores in refractory group were significantly higher than the chronic group (P<0.05). The level of TGF-β1, MMP-9, TIMP-1, IL-1, IL-4, IL-6, IL-17, and TNF-α in the refractory group were significantly higher than the chronic group and the control group (all P<0.05). Similarly, the level of the above mentioned indexes in the chronic group were significantly higher than the control group (P<0.05). The Davos score, T&T olfactometer threshold test score, Lund-Mackay CT score, and the level of TGF-β1, MMP-9, TIMP-1, IL-1, IL-4, IL-6, IL-17, and TNF-α positively correlated with refractory chronic rhinosinusitis. Moreover, the unconditional multivariate binary logistic regression showed that the influencing factors of refractory chronic rhinosinusitis included TGF-β1, MMP-9, TIMP-1, IL-1, IL-4, IL-6, IL-17, and TNF-α.Conclusion: The findings of the present study provide evidence for TGF-β1, MMP-9, TIMP-1, IL-4, IL-6, IL-17, and TNF-α as the influencing factors of refractory chronic rhinosinusitis (AU)

Los pacientes con placas coronarias vulnerables presentan mayores niveles séricos de metaloproteinada-I / Patients with vulnerable coronary plaques have higher serum metalloproteinase-1 levels

Fundamento: Las placas ateroscleróticas que producen la mayoría de los síndromes coronarios agudos al romperse son los fibroateromas de cápsula fina, denominados placas vulnerables. Éstas pueden ser detectadas únicamente con técnicas invasivas de imagen intracoronaria. Es preciso encontrar un biomarcador no invasivo que permita identificar a los pacientes con estas placas sin necesidad de cateterismo cardiaco. La metaloproteinasa-1 es una enzima involucrada en el metabolismo de la matriz extracelular que ha sido relacionada con la ruptura de las placas ateroscleróticas. Se desconocen sus niveles séricos en pacientes con placas vulnerables. Material y métodos: Se incluyeron pacientes sometidos a cateterismo cardiaco por enfermedad coronaria estable. Se estudiaron las arterias coronarias con tomografía de coherencia óptica para detectar placas vulnerables. Se extrajeron muestras de sangre periférica y del seno coronario para analizar la concentración de metaloproteinasa-1. Resultados: Se incluyeron 51 pacientes. Trece tenían al menos un fibroateroma de cápsula fina. No se encontraron diferencias significativas en las características clínicas, perfil lipídico ni proteína C reactiva entre los pacientes con y sin placas vulnerables. Los pacientes con placas vulnerables presentaron concentraciones significativamente mayores de metaloproteinasa-1, tanto en sangre periférica (7330±5541 vs 2894±1783 pg/ml, p=0,025) como en seno coronario (6012±3854 vs 2707±1252 pg/ml, p=0,047). Conclusiones: Los pacientes con placas vulnerables presentaron niveles séricos significativamente mayores de metaloproteinasa-1. Se requieren estudios con seguimiento clínico para evaluar el valor pronóstico de la metaloproteinasa-1 sérica (AU)

Background: Most acute coronary syndromes are caused by the fracture of a vulnerable atherosclerotic plaque. These plaques are thin cap fibroatheromas, which can only be detected with invasive coronary imaging techniques. It is necessary to find a non-invasive biomarker of these vulnerable plaques in order to identify patients at risk without a coronary angiography. Metalloproteinase-1 is an enzyme involved in extracellular matrix metabolism which has been correlated with the rupture of atherosclerotic plaques. Its serum levels in patients with vulnerable plaques remain unknown. Methods: Patients with suspected stable coronary artery disease undergoing coronary angiography in our hospital were included. The coronary arteries were studied with optical coherence tomography to detect vulnerable plaques. Blood samples were taken from a peripheral vein and from the coronary sinus, to assess metalloproteinase-1 levels. Results: Fifty-one patients were included, 13 of whom had at least one vulnerable plaque. There were not significant differences in clinical characteristics, lipid profile or C reactive protein levels, between patients with or without vulnerable plaques. Patients with vulnerable plaques had significant higher metalloproteinase-1 levels both in peripheral (7330±5541 vs 2894±1783 pg/ml, p=0.025) and coronary sinus serum (6012±3854 vs 2707±1252 pg/ml, p=0.047). Conclusions: Patients with vulnerable plaques had significantly higher metalloproteinase-1 serum levels. Further studies with clinical follow up are needed to assess the prognostic value of serum metalloproteinase-1 (AU)

TGF-beta 1 y su relación con la hipertensión arterial esencial / Transforming growth factor-Beta1 in essential hypertension

Introducción. Se ha sugerido que los niveles plasmáticos elevados del factor de crecimiento transformante beta 1 (TGF-beta 1) juegan un papel clave para el desarrollo de las enfermedades que cursan con fibrosis. Objetivos. Este estudio observacional, transversal, retrospectivo de casos controles busca evaluar si existe asociación entre los niveles plasmáticos de TGF-beta 1 como factor causal en la patogénesis de la hipertensión arterial (HT-A) esencial y la lesión de órganos diana, en pacientes con HT-A esencial refractaria comparado con un grupo de sujetos sanos y comprobar si existe una correlación entre los niveles de TGF-beta 1 y los de PICP (propéptido C-terminal de la molécula de procolágeno tipo I), de degradación MMP-1 (metaloproteinasa de la matriz tipo 1) y degradación del colágeno ICTP (telopéptido C-terminal de la molécula de colágeno tipo I) en el grupo de sujetos hipertensos. Pacientes y métodos. Se estudiaron 52 pacientes diagnosticados de HT-A con edad media de 53 años y se comparó con grupo control de 24 voluntarios sanos con edad media de 45 años. TGF-beta 1 fue medido por método ELISA previa activación de la muestra. Resultados. Las concentraciones de TGF-beta 1 no fueron mayores en el grupo de hipertensos. La media±desviación estándar fue de 40±13 pg/mL, mientras que en el grupo control fue de 50±23 pg/mL. La t de Student no estableció diferencias significativas. Conclusiones. no se han encontrado niveles elevados de TGF-beta 1 en pacientes con hipertensión esencial en contra de lo evidenciado por otros autores. La exclusión en este estudio de pacientes que presentaban insuficiencia renal moderada o severa, asumimos que pudiera ser la causa de no encontrar elevados los niveles plasmáticos de TGF-beta (AU)

Introduction. It has been suggested that elevated serum transforming growth factor beta1 (TGF-beta 1) levels plays a key role to develope of diseases associated with fibrosis. Objective. This observational, transversal, retrospective case control study was designed to evaluate the association between TGF-beta 1 as causal factor for the hypertension arterial pathogenesis and damage on target organ. This study was designed to evaluate the association between circulating biomarkers of collagen metabolism and fibrosis in serum to compare TGF-beta1 levels obtained in a group of essential hypertension patients with a group of normotensive healthy subjects. Patients and methods. 46 essential hypertension patients, mean age: 53 years versus a control group of 20 healthy volunteers, mean age: 45 years. TGFâ1 was quantitated by a commercial ELISA technique, samples were previously activated. Results. TGF-beta 1 concentrations were not higher in essential hypertension patients, mean serum concentration (40±13ng/mL) than in normal group, mean serum concentration (50±23ng/mL). No significant differences were showed between two groups using t- student test. Conclusion. We have not found elevated TGF-beta 1 concentrations in essential hypertension patients in contrast to what has been shown by other authors. Exclusion of patients with mild or severe renal insufficiency should be considered to be a cause for not obtaining elevated TGF-beta 1 concentrations(AU)