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1.
J. investig. allergol. clin. immunol ; 32(5): 357-366, 2022. ilus, tab
Artigo em Inglês | IBECS | ID: ibc-212731

RESUMO

Hymenoptera venom immunotherapy (VIT) is effective for protecting individuals with systemic allergic reactions caused by Hymenopterastings. The need for a tool that shows the degree of protection afforded by VIT and the lack of useful biomarkers have made the stingchallenge test (SCT) the gold standard for this disorder, although its use has both lights and shadows. SCT with Hymenoptera involvescausing a real sting in a patient diagnosed with allergy to the venom of the stinging insect and who is undergoing treatment with specificimmunotherapy. In Spain, SCT is included in the list of services offered by some hospitals and forms part of their daily clinical practice. Thisreview aims to analyze the strengths and weaknesses of this test and to describe the standardized procedure and necessary resources,based on the experience of a group of Spanish experts and a review of the literature. (AU)


La inmunoterapia con veneno de himenóptero (ITV) es un tratamiento que se ha mostrado eficaz en la protección de sujetos con reaccionesalérgicas sistémicas por picaduras de himenópteros. La necesidad de una herramienta que demuestre el grado de protección proporcionadapor la ITV, y la ausencia de biomarcadores útiles, convierte a la Prueba de Provocación con Repicadura (PPR) en el gold standard en estapatología, con sus luces y sus sombras. La PPR con himenópteros es una prueba que consiste en provocar una picadura real, a un pacienteque ha sido diagnosticado de alergia al veneno del insecto picador y habitualmente está en tratamiento con inmunoterapia específica.En España, la PPR se incluye en la cartera de servicios de algunos hospitales, formando parte de su práctica clínica habitual. Esta revisióntrata de analizar las fortalezas y debilidades de esta prueba, integrando el procedimiento estandarizado y recursos necesarios, basándoseen la experiencia de un grupo de expertos españoles y en la revisión de la literatura. (AU)


Assuntos
Humanos , Animais , Venenos de Artrópodes/uso terapêutico , Dessensibilização Imunológica/métodos , Hipersensibilidade/terapia , Mordeduras e Picadas de Insetos , Venenos de Abelha/uso terapêutico , Biomarcadores
2.
J. investig. allergol. clin. immunol ; 32(6): 451-459, 2022. graf
Artigo em Inglês | IBECS | ID: ibc-213396

RESUMO

Background: Although exposure to stings has been identified as the leading risk factor for anaphylaxis due to Hymenoptera venom allergy, professional beekeepers receive hundreds of stings yearly without developing systemic reactions. Objective: This study aims to analyze the mechanisms underlying bee venom tolerance in beekeepers. Methods: A cross-sectional study was conducted. Participants were recruited and classified into 3 groups: allergic patients (APs), who experienced systemic reactions after bee stings, with a positive intradermal test and specific IgE (sIgE) to Apis mellifera venom (AmV); tolerant beekeepers (TBKs), who received ≥50 stings/year; and healthy nonexposed controls (HCs). We measured serum levels of sIgE and specific IgG4 (sIgG4) to AmV, rApi m 1, rApi m 2, rApi m 3, Api m 4, rApi m 5, and rApi m10, as well as AmV-induced basophil degranulation, percentage of T-cell subsets, regulatory T cells (Treg), and IL-10 production. Results: Compared with TBKs, APs had high levels of sIgE to AmV and all its allergic components (P<.001), together with a high basophil activation rate (P<.001). Conversely, compared with APs, TBKs had higher levels of sIgG4 (P<.001) and IL-10 (P<.0001), as well as an enhanced CTLA-4+ Treg population (P=.001), expanded Helios– Treg (P<.003), and reduced type 1 helper T cells (TH1) (P=.008), TH2 (P=.004), and TH17 (P=.007) subsets. Conclusions: The profile of TBKs, which was strongly marked by Treg activity, differed from that of TBKs. This natural tolerance would be led by the expansion of inducible Helios– Treg cells at the peripheral level. The Helios– Treg population could be a novel candidate biomarker for monitoring tolerance (AU)


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Idoso , Venenos de Abelha/imunologia , Criação de Abelhas , Exposição Ocupacional , Tolerância Imunológica , Linfócitos T Reguladores , Estudos Transversais , Imunoglobulina E/imunologia , Imunoglobulina G/imunologia
3.
J. investig. allergol. clin. immunol ; 32(2): 116-123, 2022. ilus, graf, tab
Artigo em Inglês | IBECS | ID: ibc-203901

RESUMO

Background: Component-resolved diagnosis plays a key role in the diagnosis and treatment of honeybee venom allergy (HVA). Our aimwas to study whether any of the allergens not included in the usual diagnostic platforms are relevant in our population.Patients and Methods: The allergenic sensitization profile of Spanish patients who experienced a systemic reaction after a honeybee stingand were diagnosed with HVA was studied by immunoblotting based on raw autochthonous Apis mellifera venom characterized usingSDS-PAGE and mass spectrometry and a commercial assay (ImmunoCAP).Results: Allergens in the International Union of Immunological Societies database were detected in the raw A mellifera venom extract used,except Api m 12. Sera from 51 patients with a median (IQR) age of 46.2 years (35.6-54.6) were analyzed. ImmunoCAP revealed Api m 1and Api m 10 to be major allergens (88.2% and 74.5%, respectively). Moreover, Api m 6 (85.4%) was detected by immunoblotting.Conclusion: Api m 1, Api m 6, and Api m 10 are major A mellifera venom allergens in our population (AU)


Antecedentes: El diagnóstico molecular puede ser una herramienta valiosa en el diagnóstico y el tratamiento de la alergia al veneno deabeja. Este estudio investiga si alguno de los alérgenos no incluidos en las plataformas diagnósticas habituales son relevantes en nuestrapoblación.Pacientes y métodos: Estudiamos mediante immunoblotting el perfil de sensibilización alergénica en pacientes españoles diagnosticadosde alergia al veneno de abeja. Los resultados se compararon con los obtenidos usando un ensayo comercial (ImmunoCAP). El venenocrudo de Apis mellifera autóctona se obtuvo y caracterizó mediante SDS-PAGE y espectrometría de masas.Resultados: Los alérgenos descritos en la base de datos International Union of Immunological Societies (IUIS) fueron detectados enel extracto crudo de veneno de A. mellifera utilizado. Se analizaron sueros de 51 pacientes con una edad media de 46,2 años (rangointercuartil 35,6–54,6). Api m 1 y Api m 10 fueron detectados como alérgenos mayoritarios (88,2% y 74,5%, respectivamente) usandoImmunoCAP. Además, se encontró Api m 6 (85,4%) mediante immunoblotting.Conclusión: Nuestra población reconoce Api m 1, Api m 6 y Api m 10 como alérgenos mayoritarios del veneno de A. mellifera (AU)


Assuntos
Humanos , Animais , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Hipersensibilidade/diagnóstico , Mordeduras e Picadas de Insetos/diagnóstico , Venenos de Abelha , Alérgenos , Abelhas , Imunoglobulina E , Mordeduras e Picadas de Insetos/imunologia
4.
Allergol. immunopatol ; 48(6): 582-588, nov.-dic. 2020. tab
Artigo em Inglês | IBECS | ID: ibc-199246

RESUMO

BACKGROUND: Apitherapy represents a certain form of complementary and alternative medicine that uses bee products in combination with other methods from this field. One of the basic concepts of this type of medicine is that all diseases can be treated using apitherapy. This study was performed to assess the recommendations from authors of books on apitherapy regarding the treatment of seasonal allergic rhinitis and compare them to findings from the scientific literature. METHODS: One hundred and twenty-nine books on apitherapy were analysed regarding recommendations for allergic seasonal rhinitis. Scientific evidence regarding the efficacy of using various bee products was searched via PubMed and JUSTfind. RESULTS: Only 38.8% of the apitherapy books mentioned seasonal allergic rhinitis. Among these books, we found 29 different recommendations in favour of bee products and one against the use of honey. The most reasonable recommendation according to clinical studies on the subject, namely the use of a mix of honey and pollen, was only found once (0.8%). CONCLUSIONS: The large discrepancies and number of different recommendations demonstrate that apitherapy is not a consistent type of medicine. The recommendations regarding seasonal allergic rhinitis in the vast majority of apitherapy books cannot be considered adequate when compared to the scientific findings


No disponible


Assuntos
Humanos , Rinite Alérgica Sazonal/terapia , Apiterapia/métodos , Livros de Texto como Assunto , Mel , Pólen , Venenos de Abelha , Terapias Complementares
5.
Allergol. immunopatol ; 48(6): 804-809, nov.-dic. 2020. tab, mapas
Artigo em Inglês | IBECS | ID: ibc-199274

RESUMO

Hymenoptera venom allergy (HVA) is one of the most frequent causes of anaphylaxis following a bee, vespid or ant sting. Real-life data regarding the management of HVA in children are lacking. To address this unmet need, we carried out a survey defining the current management of HVA in children among pediatric allergists in Italy. Educational investments on the improvement of the management of pediatric patients with HVA are urgently needed, and our analysis represents a relevant instrument in targeting a roadmap with this aim. The time for pediatric allergists to take action has come, and a task force from the Rare Allergic Diseases Commission of the Italian Society of Pediatric Allergy and Immunology is working on the topic to improve pediatricians' knowledge and optimize the care of these patients


No disponible


Assuntos
Humanos , Criança , Venenos de Vespas/efeitos adversos , Venenos de Abelha/efeitos adversos , Venenos de Formiga/efeitos adversos , Hipersensibilidade/etiologia , Mordeduras e Picadas de Insetos/complicações , Himenópteros , Venenos de Vespas/imunologia , Venenos de Abelha/imunologia , Venenos de Formiga/imunologia , Hipersensibilidade/terapia , Dessensibilização Imunológica/métodos , Itália
9.
Rev. esp. investig. quir ; 20(2): 59-61, 2017. tab
Artigo em Espanhol | IBECS | ID: ibc-164591

RESUMO

El síndrome de Kounis (SK) fue descrito en 1991 por Kounis y Zavras como la aparición simultánea de eventos coronarios agudos y reacciones alérgicas anafilácticas o anafilactoides. Engloba conceptos como el de angina alérgica e infarto alérgico y se ha descrito en relación con picaduras de insectos, ingesta de fármacos y alimentos, exposiciones ambientales y condiciones médicas varias. Se desconoce su incidencia real ya que, la mayoría de la información proviene de casos clínicos o pequeñas series


Kounis syndrome was described in 1991 by Kounis and Zavras as the coincidental occurrence of acute coronary syndromes with allergic reactions (anaphylactic or anaphylactoid). Today, allergic angina and allergic myocardial infarction are referred to as Kounis syndrome, and the latter has been reported in association with a variety of drugs, insect stings, food, environmental exposures and medical conditions, among other factors. The incidence is not known, as most of the available information comes from case reports or small case series


Assuntos
Humanos , Masculino , Adulto , Doença das Coronárias/etiologia , Anafilaxia/complicações , Hipersensibilidade/complicações , Venenos de Abelha/efeitos adversos , Nitroglicerina/uso terapêutico , Mordeduras e Picadas de Insetos/complicações , Vasodilatadores/uso terapêutico
10.
J. investig. allergol. clin. immunol ; 27(1): 19-31, 2017. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-160494

RESUMO

In this review, the Hymenoptera Allergy Committee of the SEAIC analyzes the most recent scientific literature addressing problems related to the diagnosis of hymenoptera allergy and to management of venom immunotherapy. Molecular diagnosis and molecular risk profiles are the key areas addressed. The appearance of new species of hymenoptera that are potentially allergenic in Spain and the associated diagnostic and therapeutic problems are also described. Finally, we analyze the issue of mast cell activation syndrome closely related to hymenoptera allergy, which has become a new diagnostic challenge for allergists given its high prevalence in patients with venom anaphylaxis (AU)


En esta revisión el Comité de Alergia a Himenópteros de la SEAIC ha analizado la literatura científica más reciente sobre los principales problemas diagnósticos de la alergia a himenópteros, así como sobre las dificultades que pueden surgir durante la inmunoterapia con venenos. Se revisan especialmente las novedades relacionadas con el diagnóstico molecular y los perfiles moleculares de riesgo. También se describe la alergia a himenópteros poco habituales y los problemas diagnósticos y terapéuticos que esta conlleva. Por último, se tratan los síndromes de activación mastocitaria clonal, íntimamente relacionados con la alergia a himenópteros, que se han convertido en un nuevo reto diagnóstico para el alergólogo (AU)


Assuntos
Humanos , Masculino , Feminino , Alergia e Imunologia/instrumentação , Hipersensibilidade/diagnóstico , Comitê de Profissionais/organização & administração , Comitê de Profissionais/normas , Biologia Molecular/métodos , Imunoterapia/métodos , Mordeduras e Picadas de Insetos/imunologia , Himenópteros , Mastocitose/complicações , Mastocitose/diagnóstico , Mastocitose/imunologia , Anafilaxia/diagnóstico , Anafilaxia/imunologia , Anafilaxia/terapia , Venenos/imunologia , Venenos de Abelha/imunologia
11.
Allergol. immunopatol ; 44(3): 232-240, mayo-jun. 2016. graf
Artigo em Inglês | IBECS | ID: ibc-152079

RESUMO

BACKGROUND: Gramineae bee-collected pollen is identified as being at the origin of allergic accidents but the biological potency of Gramineae bee-collected pollen is not well known. Cereal grasses (e.g., Zea) and European wild forage grasses (FG) are contained in bee-collected pollen. METHOD: In this experiment, Zea-mass and FG-mass were identified in bee pollen mass and the proportion of Zea and of FG was calculated using the bee pollen melissopalynology spectrum. Skin reactivity to Zea and to FG were assessed by measuring wheal diameters (W) from skin prick tests using three serial dilutions of bee-collected pollen on 10 allergic patients to Gramineae, in order to calculate the relationship between Zea mass (Masszea) or FG mass (MassFG) in bee pollen and skin reactivity. RESULTS: The linear function Log10(WFG) = 0.24(Log10(MassFG)) + 0.33 (R = 0.99) was established using a bee pollen sample with 0.168 mg of FG pollen per mg. The linear function Log10(Wzea) = 0.23(Log10(Masszea)) + 0.14 (R = 0.99) was established using a bee pollen sample with 0.983 mg of Zea pollen per mg. Gramineae allergens seem to be little altered by bee secretions. Gramineae bee pollen retains its allergenic capacity but it depends on the members of the Gramineae family. CONCLUSIONS: To our knowledge this is the first time it has been shown that skin reactivity to Gramineae is proportional to the absolute Gramineae mass contained in the bee-collected pollen and that it depends on the members of the Gramineae family


No disponible


Assuntos
Humanos , Masculino , Feminino , Pólen/efeitos adversos , Pólen/imunologia , Pólen/fisiologia , Rinite Alérgica Sazonal/diagnóstico , Rinite Alérgica Sazonal/imunologia , Rinite Alérgica Sazonal/patologia , Zea mays/efeitos adversos , Zea mays/imunologia , Zea mays/envenenamento , Poaceae/imunologia , Poaceae/envenenamento , Poaceae/toxicidade , Venenos de Abelha/efeitos adversos , Venenos de Abelha/envenenamento , Abelhas/imunologia , Abelhas/fisiologia , Abelhas/patogenicidade , Alérgenos/efeitos adversos , Alérgenos/análise , Alérgenos/imunologia
12.
J. investig. allergol. clin. immunol ; 26(1): 40-47, 2016. tab, graf
Artigo em Inglês | IBECS | ID: ibc-150188

RESUMO

Background: The ultrarush protocol is an attractive approach in the buildup phase of venom immunotherapy (VIT-UR). However, the degree of risk of VIT-UR in children remains unknown. The objective of this study was to compare the safety of VIT-UR in children and adults. Methods: We performed a study based on prospectively gathered medical records of children and adults with hymenoptera venom allergy treated with VIT-UR in 3 allergy centers in Poland. Results: The study population comprised 134 children (mean [SD] age, 12.6 [3.7] years; males, 70.1%) and 207 adults (mean age, 42.4 [14.0] years; males, 47.8%). The number of children in the subgroups of bee venom (BV) allergy and wasp venom (WV) allergy were comparable, although sensitization to WV was more predominant in the adult group (70.1%). Skin reactivity to both venoms was more common in children than in adults (P<.001); however, children had higher concentrations of total IgE and specific IgE to BV (both P<.001). Systemic allergic reactions (VIT-SARs) occurred in 6.2% of the patients (3.7% in children and 7.7% in adults; nonsignificant). In adults, SARs occurred more frequently in patients treated with BV than WV extracts (21.4% vs 2.6%; P<.001). The same pattern was observed in children (7.2% vs 0%; P=.058). However, VIT-SARs to BV were less frequent in children than in adults (P=.034). Similarly, no significant relationship was noted between children and adults receiving WV VIT (2.6% vs 0%; nonsignificant). The severity of VIT-SAR did not differ between children and adults. Conclusions: VIT-UR is safer in children. Age below 18 is not a risk factor for VIT-SARs (AU)


Introducción: Los protocolos ultra rápidos son considerados de utilidad para realizar la fase de inicio de la inmunoterapia con venenos de himenópteros (VIT-UR). La seguridad de estos protocolos VIT-UR en los niños sigue siendo una cuestión debatida. El objetivo de este estudio fue comparar la seguridad de VIT-UR en niños y adultos. Métodos: Estudio prospectivo de seguimiento de la seguridad de la inmunoterapia en niños y adultos regularmente tratados con VIT-UR seguidos en tres unidades de alergia en Polonia. Resultados: En el estudio fueron incluidos un total de 134 niños (edad media de 12,6 años, SD 3,7; varones 70,1%) y 207 adultos (edad media 42,4 años, SD 14,0; 47,8% varones). El número de niños sensibilizados a veneno de abeja (BV) era comparable al de los sensibilizados a veneno de avista (WV), mientras que la sensibilidad al veneno de avispa prevaleció en el grupo de adultos (70,1%). Los niños con hipersensibilidad a venenos (HVA) mostraron menor reactividad cutánea a ambos venenos que los adultos con HVA (p <0,001) pero, por el contrario, en comparación con los adultos presentan concentraciones de IgE sérica total e IgE específica frente a BV (ambas p <0,001). Un 6,2% de todos los pacientes (3,7% de los niños y 7.7% de los adultos, NS) presentaron reacciones alérgicas sistémicas frente a la inmunoterapia con venenos (VIT-SAR). En los adultos, el SARS fueron más frecuentes en los pacientes tratados con BV que los tratados con WV (21,4% frente a 2,6% p <0,001). El mismo patrón se produjo en niños (7,2% vs 0%; p = 0,058). Sin embargo, las VIT-SAR frente a inmunoterapia con BV fueron menos frecuentes en los niños que en adultos (p = 0,034). Del mismo modo la frecuencia de reacciones frente a WV VIT fue menor en niños que en adultos pero sin diferencias significativas (0% vs 2,6%, NS). La gravedad de las VIT-SAR fue similar para niños y adultos. Conclusiones: Los protocolos VIT-UR son más seguros en los niños. Edad menor de 18 años no es un factor de riesgo de VIT-SAR (AU)


Assuntos
Humanos , Masculino , Feminino , Criança , Adulto , Venenos de Abelha/imunologia , Venenos de Abelha/uso terapêutico , Imunoterapia/instrumentação , Imunoterapia/métodos , Venenos de Vespas , Venenos de Vespas/imunologia , Venenos de Vespas/uso terapêutico , Hipersensibilidade Imediata/imunologia , Imunoglobulina E/imunologia , Receptores de IgE/imunologia , Protocolos Clínicos , Estudos Prospectivos , Seguimentos , Imunização/métodos , Imunização , Fatores de Risco
13.
J. investig. allergol. clin. immunol ; 26(6): 355-361, 2016. tab, graf
Artigo em Inglês | IBECS | ID: ibc-159022

RESUMO

Background and Objective: Different clinical behaviors have been identified in patients allergic to bee venom. Compound-resolved diagnosis could be an appropriate tool for investigating these differences. The aims of this study were to analyze whether specific IgE to Api m 4 (sIgE-Api m 4) can identify a particular kind of bee venom allergy and to describe response to bee venom immunotherapy (bVIT). Methods: Prospective study of 31 patients allergic to bee venom who were assigned to phenotype group A (sIgE-Api m 4 <0.98 kU/L), treated with native aqueous (NA) extract, or phenotype group B (sIgE-Api m 4 ≥0.98 kU/L), treated with purified aqueous (PA) extract. Sex, age, cardiovascular risk, severity of preceding sting reaction, exposure to beekeeping, and immunological data (intradermal test, sIgE/ sIgG4-Apis-nApi m 1, and sIgE-rApi m 2-Api m 4 were analyzed. Systemic reactions (SRs) during bVIT build-up were analyzed. Immunological and sting challenge outcomes were evaluated in each group after 1 and 2 years of bVIT. Results: Phenotype B patients had more severe reactions (P=.049) and higher skin sensitivity (P=.011), baseline sIgE-Apis (P=.0004), sIgE-nApi m 1 (P=.0004), and sIgG4-Apis (P=.027) than phenotype A patients. Furthermore, 41% of patients in group B experienced SRs during the build-up phase with NA; the sting challenge success rate in this group was 82%. There were no significant reductions in serial intradermal test results, but an intense reduction in sIgE-nApi m 1 (P=.013) and sIgE-Api m 4 (P=.004) was observed after the first year of bVIT. Conclusion: Use of IgE-Api m 4 as the only discrimination criterion demonstrated differences in bee venom allergy. Further investigation with larger populations is necessary (AU)


Antecedentes y Objetivos: Los pacientes alérgicos a veneno de abeja muestran distintos comportamientos clínicos. El diagnóstico por componentes podría ayudar a entenderlos. El objetivo de este estudio fue estudiar la capacidad de la IgE-Api m4 para identificar diferentes patrones de alergia al veneno de abeja, incluyendo la respuesta a la inmunoterapia al veneno de abeja (ITVa). Métodos: Estudio prospectivo de 31 pacientes alérgicos al veneno de abeja, distribuidos en dos grupos fenotípicos (A y B) en función de sus niveles de IgE-Api m4 (punto de corte 0,98 kU/L) y tratados con extracto acuoso nativo (AN)-fenotipo A, o extracto acuoso purificado (AP)-fenotipo B. Se analizaron sexo, edad, riesgo cardiovascular, gravedad de la picadura, exposición y datos inmunológicos (intradermorreación, IgE e IgG4-Apis-nApi m1 e IgE-rApi m2-Api m4). Se analizó la seguridad en la fase de inicio de la ITVa, y la eficacia y cambios inmunológicos después de 1 y 2 años de ITVa. Resultados: El fenotipo-B mostró reacciones más graves con las picaduras (p=0,049), una mayor sensibilidad cutánea (p=0,011) y valores más elevados de IgE-Apis (p=0,0004), IgE-nApim1 (p=0,0004), e IgG4-Apis (p=0,027) que el fenotipo-A. Por otra parte, el 41% de los pacientes del fenotipo-B sufrió reacciones sistémicas durante el inicio con AN, con una tasa de protección del 82%. La respuesta cutánea no mejoró significativamente, y se comprobó la reducción intensa de IgE-nApi m1 (p=0,013) e IgE-Api m4 (p=0,004) desde el primer año de ITVa. Conclusión: El uso de la IgE-Api m4 como único criterio discriminativo ha podido confirmar que hay diferentes maneras de ser alérgico al Va. Se necesitan estudios en poblaciones más amplias (AU)


Assuntos
Humanos , Masculino , Feminino , Venenos de Abelha/efeitos adversos , Venenos de Abelha/imunologia , Hipersensibilidade Imediata/imunologia , Imunoglobulina E/análise , Imunoglobulina E/imunologia , Alérgenos/imunologia , Dessensibilização Imunológica/métodos , Mordeduras e Picadas/imunologia , Mordeduras e Picadas de Insetos/imunologia , Estudos Prospectivos , Imunoterapia/instrumentação , Imunoterapia
14.
J. investig. allergol. clin. immunol ; 25(6): 426-430, 2015. tab, graf, mapas
Artigo em Inglês | IBECS | ID: ibc-146914

RESUMO

Background and Objective: The Spanish version of the health-related quality of life questionnaire for allergy to hymenoptera venom (HRQLHA) has been shown to be reliable, internally consistent, and externally valid. The aim of this study was to complete the validation of the HRQLHA by analyzing its sensitivity to changes (longitudinal validity) using the sting challenge test (SCT) as the variable of change. Patients and Methods: Patients over the age of 17 years with a systemic allergic reaction to Apis, Vespula, or Polistes venom were included during their first year of venom-specific immunotherapy. Patients were assigned to either a group that underwent the SCT or a control group that did not. All patients completed the HRQLHA at baseline and after a period of 2 to 4 months, during which time the SCT was performed in the active group, with no intervention in the control group. Results: Fifty patients were included in the study: 25 in the SCT group and 25 in the control group. The patients in the SCT group showed a significant improvement in mean HRQLHA score (+0.35, P=.03) after the SCT, while those in the control group showed no significant changes in questionnaire scores. Conclusions: Our results demonstrate the sensitivity of the HRQLHA to changes and thus complete the longitudinal validation of the questionnaire. A well-tolerated SCT improves the quality of life of venom-allergic patients as it reduces anxiety associated with the fear of being stung (AU)


Introducción y objetivo: El cuestionario de calidad de vida en español para alérgicos a veneno de himenópteros (HRQLHA) ha demostrado su fiabilidad, consistencia interna y validez externa. El objetivo de este estudio era completar la validación del HRQLHA mediante el análisis de su sensibilidad al cambio o validación longitudinal, considerando como variable de cambio la prueba de repicadura controlada intrahospitalaria (RIH). Pacientes y Métodos: Se incluyeron pacientes mayores de 17 años con reacción sistémica por alergia a veneno de Apis, Vespula o Polistes, en el primer año de tratamiento con inmunoterapia. Los pacientes se distribuyeron en un grupo sometido a RIH y un grupo control no sometido a esta prueba. Los pacientes cumplimentaron el HRQLHA basal y después de la repicadura o después de un periodo de 2-4 meses si pertenecían al grupo control. Resultados: Se incluyeron 50 pacientes en el estudio. 25 en el grupo de RIH y 25 en el grupo control. Los pacientes del grupo RIH presentaron una mejoría significativa en la puntuación del cuestionario HRQLHA después de la repicadura de + 0,35 (p=0,03), mientras que los pacientes del grupo control no mostraron cambios significativos en la puntuación del cuestionario. Conclusiones: Los resultados obtenidos demuestran la sensibilidad al cambio del HRQLHA y permiten completar la validación longitudinal del cuestionario. La prueba de repicadura controlada intrahospitalaria bien tolerada mejora la calidad de vida de los pacientes alérgicos a venenos ya que disminuye su ansiedad ante una nueva picadura (AU)


Assuntos
Humanos , Venenos de Abelha/efeitos adversos , Hipersensibilidade/psicologia , Psicometria/instrumentação , Himenópteros/patogenicidade , Qualidade de Vida/psicologia , Perfil de Impacto da Doença , Estudos de Casos e Controles
18.
J. investig. allergol. clin. immunol ; 23(1): 30-36, ene.-feb. 2013. tab, ilus
Artigo em Inglês | IBECS | ID: ibc-111767

RESUMO

Antecedentes: Los niveles séricos elevados de triptasa son un marcador de gravedad de las reacciones a las picaduras de himenópteros y de las reacciones a la inmunoterapia específica. Objetivo: Investigar los niveles séricos de triptasa en apicultores alérgicos y no alérgicos al veneno de himenópteros. Métodos: Se determinó la triptasa sérica en pacientes adultos con alergia al veneno de himenópteros (n=91, 37 de los cuales eran apicultores), apicultores sin alergia al veneno de himenópteros (n=152), y en controles de una población general adulta (n=246). Resultados: En los análisis multivariante, se observó que las concentraciones de triptasa sérica estaban positivamente asociadas con el hecho de ser apicultor (P<.001) y con el hecho de ser alérgico al veneno de himenópteros (P<.001). Los niveles de triptasa sérica también se asociaron positivamente con la edad (P<.001) y el sexo masculino (P=.02), y negativamente con el consumo de alcohol (P=.002). Conclusiones: La apicultura y la alergia al veneno de himenópteros se asocian independientemente con concentraciones elevadas de triptasa sérica (AU)


Background: Increased tryptase concentrations are a risk marker for the severity of reactions to Hymenoptera stings or venom immunotherapy. Objective: To investigate serum tryptase concentrations in beekeepers with and without Hymenoptera venom allergy (HVA). Methods: Serum tryptase concentrations were measured in adult patients with HVA (n=91, 37 of whom were beekeepers), beekeepers without HVA (n=152), and control individuals from the general adult population (n=246). Results: Multivariate analyses revealed that serum tryptase levels were positively associated with beekeeping activities (P<.001) and HVA (P<.001). Tryptase levels were also positively associated with age (P<.001) and male sex (P=.02), and negatively associated with alcohol consumption (P=.002). Conclusions: Beekeeping and HVA are independently associated with increased concentrations of serum tryptase (AU)


Assuntos
Humanos , Triptases/sangue , Venenos de Abelha/efeitos adversos , Himenópteros/patogenicidade , Hipersensibilidade/imunologia , Criação de Abelhas , Biomarcadores/análise
19.
Allergol. immunopatol ; 40(1): 9-13, ene.-feb. 2012.
Artigo em Inglês | IBECS | ID: ibc-96252

RESUMO

Background Although there are some published data about the prevalence of honeybee and vespid venom allergy from Turkey, there has been no report about Hymenoptera venom immunotherapy practice. Our aim was to determine the characteristics of Hymenoptera venom hypersensitivity and venom immunotherapy practice in Ankara, Turkey. Methods Demographic and clinical data, intradermal test, and serum specific IgE results of 65 Hymenoptera venom allergic patients who were followed up in our department from February 2005 to August 2009 were analysed. Results Serum Vespula specific IgE class (p:0.02) and Apis specific IgE class were high (p<0.0001) and Apis intradermal test results were positive (p<0.001) in accordance with the patients’ history. However, intradermal test results with Vespula were not consistent with self-reported Hymenoptera type (p:0.15). While Apis specific IgE and intradermal test results were correlated with each other (rho: 0.59, p<0.0001), Vespula specific IgE and intradermal test results were not (rho: 0.2, p:0.17). Intradermal test against Vespula did not discriminate between Apis and Vespula hypersensitive patients. There were no significant differences when the grade of reaction and specific IgE and intradermal test results were compared between Apis and Vespula. Conclusions Vespula venom hypersensitivity was more common among our patients. However, intradermal tests with Vespula had limited diagnostic sensitivity and were not correlated with serum specific IgE. Based on our results and previous reports, we recommend that negative skin test responses, especially with Vespula, need further investigation(AU)


Assuntos
Humanos , Himenópteros/patogenicidade , Venenos de Abelha/efeitos adversos , Hipersensibilidade/imunologia , Venenos de Vespas/efeitos adversos , Testes Cutâneos
20.
Rev. toxicol ; 28(2): 147-151, jul.-dic. 2011. tab, ilus
Artigo em Espanhol | IBECS | ID: ibc-94024

RESUMO

La utilización de productos con bajo o nulo impacto en organismos circundantes y el ambiente, constituye parte del nuevo concepto de protección de cultivos. Los productos NeoNim 60 y OleoNim 80, obtenidos del árbol del Nim, están propuestos para su uso como insecticida, nematicida y acaricida. Con el objetivo de realizar la evaluación ecotoxicológica de estos productos, se realizaron los ensayos de toxicidad aguda en lombriz de tierra Eisenia andrei y de toxicidad aguda por contacto en abejas Apis mellifera. Cada ensayo contó con un grupo control y dos tratados. El ensayo en lombrices se realizó durante 14 días utilizando suelo artificial, realizando observaciones clínicas, conteo de animales y estudio del comportamiento de la biomasa. La prueba en abejas se prolongó por 48 horas, realizándose observaciones clínicas y conteo de animales. Se obtuvo un 100% de supervivencia en las lombrices de los grupos tratados, obteniendo en el NeoNim 60 valores de disminución de la biomasa significativamente inferiores al grupo control, mientras que el grupo tratado con OleoNim 80 mostró aumento en la biomasa del inicio al final del ensayo. En el ensayo en abejas, la comparación estadística no mostró diferencias entre grupos en cuanto a la mortalidad. Teniendo en cuenta el conjunto de los resultados obtenidos, se pudo concluir que los derivados del Nim NeoNim 60 y OleoNim 80 no producen efecto tóxico sobre las lombrices de tierra y las abejas (AU)


The use of products with low or null impact in surroundingorganisms and the environment constitutes part of the new concept of crop protection. The products NeoNim 60 and OleoNim 80, obtained from the Neem tree, are proposed for its use as insecticide, nematicide and acaricide. With the objective of perform the ecotoxicological assessment of these products, it were carried out the earthworm acute toxicity and the honey bee acute contact toxicity tests. For each assay two Treated and one Control group were established. The assay in earthworms Eisenia andrei was carried out during 14 days using artificial soil, with daily clinical observations, count of animals and study of the changes of the biomass. The test in honey bees Apis mellifera was prolonged for 48 hours, with daily clinical observations and count of animals. A survival of 100% was obtained in the earthworms of the Treated groups, obtaining in the NeoNim 60 group significative lower values of biomass when compared to the Control, while the group treated with OleoNim 80 showed an increase in the biomass from the beginning to the end of the assay. In the assay in bees, the statistical comparison didn't show differences among groups as for the mortality. Taken into account the obtained results, it could be concluded that the Neem derivatives NeoNim 60 and OleoNim 80 don't produce toxic effect on the earthworms and the honeybees (AU)


Assuntos
Animais , Masculino , Feminino , Ecotoxicologia/organização & administração , Ecotoxicologia/normas , Oligoquetos/patogenicidade , Oligoquetos/efeitos da radiação , Venenos de Abelha/toxicidade , Abelhas/patogenicidade , Ecotoxicologia/métodos , Ecotoxicologia/estatística & dados numéricos , Compostagem com Minhocas/estatística & dados numéricos
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