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1.
Clin. transl. oncol. (Print) ; 19(4): 477-488, abr. 2017. tab, ilus, graf
Artigo em Inglês | IBECS | ID: ibc-160898

RESUMO

Background. The role of the interaction between tumor cells and inflammatory cells in gallbladder carcinoma (GBC) is unclear. Inflammatory cells exist in both the tumor immune microenvironment and the host peripheral blood circulatory system. In the current study, we examined the prognostic value of inflammatory cells in the tumor microenvironment and peripheral blood in patients with GBC. Methods. 98 patients with GBC were recruited in this retrospective study. Using immunohistochemistry, we examined tumor-infiltrating CD3+ generic T-cells, CD8+ cytotoxic T-cells, CD45RO+ memory T-cells, and CD15+ neutrophils. Peripheral venous blood samples were also collected, and absolute neutrophil count (ANC), absolute lymphocyte count (ALC) and neutrophil/lymphocyte ratio (NLR) were measured. The relationships between these variables and patient outcome were evaluated. Results. Survival analysis revealed that the density of CD3+ cell infiltrates in the tumor microenvironment was positively correlated with overall survival (OS) and the density of CD15+ cell infiltrates was negatively correlated with the OS. The combined analysis showed that a high density of CD3+ cell infiltrates combined with a low density of CD15+ cell infiltrates was an independent prognostic factor for GBC. In peripheral blood, survival analysis suggested that ANC and NLR were negatively correlated, while ALC was positively correlated with OS. Multivariate survival analysis showed that NLR was an independent prognostic factor for gallbladder cancer prognosis. Conclusions. The results indicate that the combination of high density of CD3+ cell infiltrates combined with a low density of CD15+ cell infiltrates in tumor samples and pretreatment peripheral blood NLR were independent prognostic factors in patients with GBC (AU)


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Assuntos
Humanos , Masculino , Feminino , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Neoplasias da Vesícula Biliar/tratamento farmacológico , Neoplasias da Vesícula Biliar/radioterapia , Prognóstico , Carcinoma/complicações , Carcinoma/patologia , Imuno-Histoquímica/métodos , Imuno-Histoquímica , Estudos Retrospectivos , Complexo CD3/análise , Neoplasias da Vesícula Biliar/patologia , Antígenos CD15/análise , Declaração de Helsinki , 28599 , Adenocarcinoma/complicações , Análise Multivariada
2.
Rev. esp. enferm. dig ; 107(10): 598-607, oct. 2015. tab, ilus
Artigo em Inglês | IBECS | ID: ibc-141423

RESUMO

BACKGROUND: Although colorectal carcinogenesis has been intensively studied, the published investigations do not provide a consistent description of how different carbohydrate determinants of colorectal epithelium are modified in colorectal cancer (CRC). OBJECTIVE: This study is an attempt to characterize the terminal fucosylation steps responsible for the synthesis of mono- (Lea/Lex) and difucosylated (Leb/Ley) Lewis antigens in healthy and tumour CRC tissue. METHODS: An immunohistochemical study of Lewis antigens' expression was undertaken, along with screening of the fucosyltransferase (FT) activities involved in their synthesis, on healthy and tumour samples from 18 patients undergoing CRC. RESULTS: Analysis of a(1,2/3/4)FT activities involved in the sequential fucosylation of cores 1 and 2 showed significant increases in tumour tissue. Expressed as mU/mg and control vs. tumour activity (p from Wilcoxon's test), the FT activities for Lea/ Leb synthesis were: lacto-N-biose a(1,2)/a(1,4)FT, 65.4 ± 19.0 vs. 186 ± 35.1 (p < 0.005); lacto-N-fucopentaose 1 a(1,4)FT, 64.9 ± 11.9 vs. 125.4 ± 20.7 (p < 0.005); Lea a(1,2)FT, 56.2 ± 7.2 vs. 130.5 ± 15.6 (p < 0.001). Similarly, for Lex/Ley synthesis were: N-acetyllactosamine a(1,2)-/a(1,3)FT, 53.4 ± 12.2 vs. 108.1 ± 18.9 (p < 0.001); 2'-Fucosyl-N-acetyllactosamine a(1,3)FT, 61.3 ± 10.7 vs. 126.4 ± 22.9 (p < 0.001); 2'-Fucosyllactose a(1,3)FT, 38.9 ± 10.9 vs. 143.6 ± 28.9 (p < 0.001); 2'-Methyllactose a(1,3) FT, 30.9 ± 4.8 vs. 66.1 ± 8.1 (p < 0.005); and Lex a(1,2)FT, 54.3 ± 11.9 vs. 88.2 ± 14.4 (p < 0.001). Immunohistochemical Ley expression was increased (p < 0.01 according to Wilcoxon's test) in tumour tissue, with 84.6% of specimens being positive: 7.7% weak, 15.4% moderate and 61.5% high intensity. CONCLUSIONS: Results suggest the activation of the biosynthesis pathways of mono- and difucosylated Lewis histo-blood antigens in tumour tissue from CRC patients, leading to the overexpression of Ley, probably at the expense of Lex


No disponible


Assuntos
Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias Colorretais/diagnóstico , Neoplasias Colorretais/epidemiologia , Antígenos CD15/análise , Antígenos CD15/isolamento & purificação , Imuno-Histoquímica/métodos , Imuno-Histoquímica , Fucosiltransferases/análise , Fucosiltransferases , Oligossacarídeos , Carcinogênese/patologia , Estatísticas não Paramétricas , Recidiva Local de Neoplasia/diagnóstico
3.
Clin. transl. oncol. (Print) ; 17(1): 50-56, ene. 2015. tab, ilus
Artigo em Inglês | IBECS | ID: ibc-131904

RESUMO

Purpose. The role of interleukin-17 (IL-17) in the tumor microenvironment is controversial. We analyzed the in situ tumor expression of IL-17 in colorectal cancer (CRC), adenoma and non-tumor tissue to explore the possible correlation of IL-17 expression to clinicopathological characteristics, tumor-infiltrating neutrophils (TINs) and survival in CRC. Methods. We reviewed the records of 78 consecutive patients diagnosed with CRC. Archival tissues were used. Thirty-six patients with colorectal adenoma were also included. From the 78 CRC patients, we randomly chose 40 cases and collected non-tumor tissue at 10 cm from the edge of the resected tumor. Immunohistochemistry was performed using anti-IL-17 and anti-CD15 (targeting neutrophils) antibody, respectively. Real-time PCR was used to detect IL-17 mRNA in different tissues. Associations between IL-17 expression, clinicopathological parameters and prognosis were evaluated. Results. The level of IL-17 mRNA was higher in CRC than in adenoma and non-tumor tissue (P < 0.05). Positive IL-17 protein expression was observed more frequently in CRC as compared to colorectal adenoma and non-tumor tissue, respectively (P < 0.01). IL-17 expression correlated to well differentiation and early stage CRC. The number of CD15+ neutrophils significantly increased in CRC and positively correlated to the expression of IL-17 (P < 0.05). Both Kaplan–Meier analysis and multivariate Cox regression analysis indicated that patients with positive IL-17 expression showed better overall survival. Conclusions. The association between IL-17 expression and the clinicopathological parameters, as well as the clinical outcome suggests a significant role of IL-17 in CRC. IL-17 is a marker of favorable prognosis (AU)


No disponible


Assuntos
Humanos , Masculino , Feminino , Pessoa de Meia-Idade , Interleucina-17 , Interleucina-17/metabolismo , Interleucina-17/farmacocinética , Biomarcadores Tumorais/antagonistas & inibidores , Neoplasias Colorretais/diagnóstico , Antígenos CD15/administração & dosagem , Antígenos CD15/análise , Quimiotaxia de Leucócito , Interleucina-17/antagonistas & inibidores , Imuno-Histoquímica/métodos , Imuno-Histoquímica , Reação em Cadeia da Polimerase/métodos , Reação em Cadeia da Polimerase/normas , Estimativa de Kaplan-Meier
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