Polysplenia syndrome - a rare case report

The aim of this study is to report a case of polysplenia syndrome observed in an 1 1/2 yr. old male patient who came for CT thorax for preoperative evaluation of congenital heart disease. There was partial anomalous pulmonary venous drainage – right lung was draining into right atrium and left lung into left atrium. The IVC was interrupted and venous return was via the continuation of enlarged azygos vein, which was running adjacent to the aorta and to its right side. The bronchial and vascular anatomy of the right lung was identical to that of the left lung, i.e, bilateral bilobed lungs with bilateral hyparterial bronchi - a variant condition called left thoracic isomerism. In the abdomen splenule was present. The pathogenesis is not completely clear, but it is thought to be caused by an abnormality of embryonic curvature with a possible genetic basis (AU)

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Interrupción aislada de la vena cava inferior / Isolated interruption of the inferior vena cava

El diagnóstico prenatal de la interrupción de vena cava inferior con continuación de la vena ácigos es infrecuente. Su hallazgo nos obligará a descartar la presencia de un síndrome de cardioesplenia o isomerismo, fundamentalmente el isomerismo izquierdo o poliesplenia, ya que la interrupción de vena cava inferior es un excelente marcador de esta afección: se encuentra en un 55-85% de los casos. Sin embargo, también puede corresponder a una malformación vascular aislada, con nula o escasa repercusión en el pronóstico vital del recién nacido, aunque luego no esté exento de posibles complicaciones en el transcurso de su vida adulta (AU)

The prenatal diagnosis of interruption of the inferior vena cava with azygos vein continuation is uncommon. Its finding will require us to rule out the presence of a cardiosplenic syndrome or isomerism, particularly left isomerism or polysplenia, as interruption of the inferior vena cava is an excellent marker of this disease, being found in 55-85% of cases. However, it can also correspond to an isolated vascular malformation, with no or little effect on the prognosis, essential in the newborn. Although there could be complications later on during their adult life (AU)

Determinación cuantitativa de indigotina e indirubina en el índigo natural mediante cromatografía líquida de alto rendimiento (HPLC) / Quantitative HPLC determination of indigotin and indirubin in indigo naturalis

Se desarrolló un método de cromatografía líquida de alto rendimiento cuantitativa y de separaciónutilizando una columna RP-18 eluida isocráticamente con metanol y agua (73:27, v/v) para analizar loscompuestos activos isoméricos indigotina e indirubina presentes en el extracto de cloroformo del índigonatural. Se utilizaron soluciones estandarizadas de indigotina e indirubina para evaluar la linealidad, laprecisión (replicabilidad), la exactitud y la especificidad del método. Los resultados mostraron unalinealidad (r2 > 0,999) de entre 1,8 y 16,2 mg/ml para la indigotina y entre 1,6 y 14,4 ìg/ml para laindirubina. También se obtuvo un alto grado de especificidad, robustez, replicabilidad (desviaciónestándar relativa inferior al 5% para estándares y extractos) y exactitud (% de recuperaciones superioral 95%)

A high-performance liquid chromatographic separation and quantitative method using a RP-18 columneluted isocratically with methanol and water (73:27, v/v) was developed to analyze the isomeric activecompounds, indigotin and indirubin, present in the chloroform extract of indigo naturalis. Standardizedsolutions of indigotin and indirubin were used to evaluate linearity, precision (repeatability), accuracyand specificity of the method were evaluated. The results exhibited a linearity (r2 > 0.999) of between1.8 - 16.2 ìg/ml and 1.6 - 14.4 mg/ml for indigotin and indirubin, respectively. A high degree ofspecificity and robustness, as well as repeatability (relative standard deviation less than 5% for bothstandards and the extracts) and accuracy (% recoveries greater than 95%) were also achieved