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1.
Int. microbiol ; 26(4): 723-739, Nov. 2023. ilus, graf
Artigo em Inglês | IBECS | ID: ibc-227464

RESUMO

Arthrobacter ureafaciens DnL1-1 is a bacterium used for atrazine degradation, while Trichoderma harzianum LTR-2 is a widely used biocontrol fungus. In this study, a liquid co-cultivation of these two organisms was initially tested. The significant changes in the metabolome of fermentation liquors were investigated based on cultivation techniques (single-cultured and co-cultured DnL1-1 and LTR-2) using an UPLC-QTOF-MS in an untargeted metabolomic approach. Principle components analysis (PCA) and partial least squares discriminant analysis (PLS-DA) supervised modelling revealed modifications of the metabolic profiles in fermentation liquors as a function of interactions between different strains. Compared with pure-cultivation of DnL1-1, 51 compounds were altered during the cocultivation, with unique and significant differences in the abundance of organic nitrogen compounds (e.g. carnitine, acylcarnitine 4:0, acylcarnitine 5:0, 3-dehydroxycarnitine and O-acetyl-L-carnitine) and trans-zeatin riboside. Nevertheless, compared with pure-cultivation of LTR-2, the abundance of 157 compounds, including amino acids, soluble sugars, organic acids, indoles and derivatives, nucleosides, and others, changed significantly in the cocultivation. Among them, the concentration of tryptophan, which is a precursor to indoleacetic acid, indoleacetic acid, aspartic acid, and L-glutamic acid increased while that of most soluble sugars decreased upon cocultivation. The fermentation filtrates of co-cultivation of LTR-2 and DnL1-1 showed significant promoting effects on germination and radicle length of wheat. A subsequent experiment demonstrated synergistic effects of differential metabolites caused by co-cultivation of DnL1-1 and LTR-2 on wheat germination. Comprehensive metabolic profiling may provide valuable information on the effects of DnL1-1 and LTR-2 on wheat growth.(AU)


Assuntos
Arthrobacter/crescimento & desenvolvimento , Trichoderma/crescimento & desenvolvimento , Técnicas de Cocultura , Metaboloma , Fermentação , Triticum , Arthrobacter/metabolismo , Trichoderma/metabolismo , Microbiologia
2.
Nutr. hosp ; 38(5)sep.-oct. 2021. tab
Artigo em Inglês | IBECS | ID: ibc-224647

RESUMO

Background: the biological activity of vitamin D depends on the activity of its receptor or VDR. On the other hand, the activity of this receptor is influenced by its state of methylation. The objective of this study was to verify if the BsmI polymorphism of the VDR gene influences its methylation profile in adolescents. Secondly, it was to verify if the status of some metabolic factors (oxidative stress, inflammation, lipid profile, and glycemia) in the serum, and gender-adjusted vitamin D levels are independent factors with an influence on the VDR methylation profile. Methods and results: the study included 198 adolescents of both sexes, aged 15-19 years, who underwent testing for VDR gene methylation polymorphisms, serum vitamin D levels, and metabolic, oxidative stress, and systemic inflammation markers. It was observed that the BB genotype was less methylated than the other groups (26.1 % versus 30.3 %, and 29.3 % for Bb and bb, respectively), although without statistical differences between them. The odds ratio indicated a protection of 13 % (partially methylated) for vitamin D status, while alpha glycols increased the risk ratio (of being partially methylated) by 3 %. MDA was protective at a 28 % chance of risk that adolescents with higher levels of lipid peroxidation would be hypomethylated. Conclusion: we conclude that the methylation profile of the VDR gene is not influenced by the different BsmI polymorphism genotypes, and that serum vitamin D and serum markers of oxidative stress and inflammation can modulate this profile. (AU)


Antecedentes: la actividad biológica de la vitamina D depende de la actividad de su receptor, el VDR. Por otro lado, la actividad de este receptor está influenciada por su estado de metilación. El objetivo de este estudio es verificar si el polimorfismo BsmI del gen VDR influye en el perfil de metilación del mismo en los adolescentes. En segundo lugar, verificar si los factores metabólicos (estrés oxidativo, inflamación, perfil lipídico y glucemia) del suero y la vitamina D ajustada por sexo actúan independientemente de los polimorfismos sobre el perfil de metilación del VDR. Métodos y resultados: el estudio incluyó a 198 adolescentes de ambos sexos, de 15 a 19 años de edad, que se sometieron a análisis de polimorfismos de metilación del gen VDR, niveles de vitamina D, marcadores metabólicos, estrés oxidativo e inflamación sistémica. Se observó que el genotipo BB estaba menos metilado que los otros grupos (26,1 % contra 30,3 % y 29,3 % para Bb y bb respectivamente), aunque sin diferencias estadísticas entre ellos. El odds ratio indicó una protección del 13 % (parcialmente metilado) para el estado de la vitamina D, mientras que los alfa glicoles aumentaron el índice de riesgo (de estar parcialmente metilado) en un 3 %. La MDA fue protectora con un 28 % de probabilidad de riesgo de que los adolescentes con niveles más altos de peroxidación lipídica fueran hipometilados. Conclusión: concluimos que el perfil de metilación del gen VDR no está influenciado por los diferentes genotipos del polimorfismo BsmI y que la vitamina D y los marcadores de estrés oxidativo e inflamación en el suero pueden modular este perfil. (AU)


Assuntos
Humanos , Masculino , Feminino , Adolescente , Inflamação/genética , Metilação , Fatores Sexuais , Receptores de Calcitriol/genética , Receptores de Calcitriol/efeitos dos fármacos , Inflamação/prevenção & controle , Metaboloma/genética , Estresse Oxidativo/genética , Polimorfismo Genético/genética
4.
Rev. esp. patol. torac ; 31(2): 138-143, jun. 2019. graf, tab
Artigo em Espanhol | IBECS | ID: ibc-183655

RESUMO

Objetivo: Evaluar en pacientes con enfermedad pulmonar obstructiva crónica respecto a pacientes con cáncer epidermoide de pulmón en estadio inicial (CP I-II) si en el metaboloma del sudor existen diferencias en los compuestos. Metodología: Se incluyeron 11 pacientes con EPOC y 9 pacientes con CP I-II. El sudor se recogió siguiendo una técnica estandarizada y la muestra fue congelada a -80ºC hasta el análisis metabolómico, para lo que se utilizó un cromatógrafo de líquidos acoplado a un espectrómetro de masas de alta resolución (LC-QTOF) con ionización por electrospray. Se realizó un análisis de cambio (AC) para detectar las diferencias de concentración relativa de metabolitos entre grupos. Resultados. Las características basales de los sujetos incluidos en los dos grupos fueron similares. En la clínica destaca que un 67% de los enfermos con CP I-II (67%) no manifestaron síntomas atribuibles al tumor. El análisis metabolómico mostró que en el análisis de cambio una tetrahexosa presentó diferencias entre el grupo de enfermos con EPOC y con CP I-II (AC: - 4,021), igual tendencia se observó en un trisacárido fosfato (AC: -1,741) y en un lípido sulfónico (AC: -1,920). Conclusión: En muestras de sudor, el análisis de cambio muestra metabolitos con potencialidad para diferenciar entre pacientes EPOC y con CP I-II. Este resultado puede tener aplicabilidad en el cribado del cáncer de pulmón


Objective: To evaluate whether there are differences in sweat metabolite compounds in patients with chronic obstructive pulmonary disease compared to patients with early-stage squamous cell lung cancer (LC I-II). Methods: 11 patients with COPD and 9 patients with LC I-II were included. Sweat was collected using a standardized technique and the sample was frozen at -80ºC until the metabolic analysis was performed, which used a liquid chromatograph coupled with a high-resolution mass spectrometer (LC-QTOF) with electrospray ionization. A change analysis (CA) was done to detect the differences in the relative concentrations of metabolites between groups. Results: The baseline characteristics of subjects included in the two groups were similar. In the clinical presentation, it is worth noting that 67% of patients with LC I-II (67%) did not show symptoms that could be attributed to the tumor. The metabolic analysis showed that in the change analysis, a tetra-hexose showed differences between the COPD group and LC I-II group (CA: -4.021), the same pattern observed in a phosphate trisaccharide (CA: -1.741) and in a sulphonic lipid (AC: -1.920). Conclusion: In sweat samples, the change analysis shows metabolites with the potential to differ between patients with COPD and those with LC I-II. This result can be applied in lung cancer screening


Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Idoso , Suor , Doença Pulmonar Obstrutiva Crônica/metabolismo , Neoplasias Pulmonares/metabolismo , Metaboloma , Espectrometria de Massas , Estudos Prospectivos , Cromatografia , Estatísticas não Paramétricas
5.
J. physiol. biochem ; 74(3): 403-416, ago. 2018. graf
Artigo em Inglês | IBECS | ID: ibc-178995

RESUMO

Diabetes mellitus (DM) is a chronic disease that can affect metabolism of glucose and other metabolites. In this study, the normal- and obese-diabetic rats were compared to understand the diabetes disorders of type 1 and 2 diabetes mellitus. This was done by evaluating their urine metabolites using proton nuclear magnetic resonance (1H NMR)-based metabolomics and comparing with controls at different time points, considering the induction periods of obesity and diabetes. The biochemical parameters of the serum were also investigated. The obese-diabetic model was developed by feeding the rats a high-fat diet and inducing diabetic conditions with a low dose of streptozotocin (STZ) (25 mg/kg bw). However, the normal rats were induced by a high dose of STZ (55 mg/kg bw). A partial least squares discriminant analysis (PLS-DA) model showed the biomarkers of both DM types compared to control. The synthesis and degradation of ketone bodies, tricarboxylic (TCA) cycles, and amino acid pathways were the ones most involved in the variation with the highest impact. The diabetic groups also exhibited a noticeable increase in the plasma glucose level and lipid profile disorders compared to the control. There was also an increase in the plasma cholesterol and low-density lipoprotein (LDL) levels and a decline in the high-density lipoprotein (HDL) of diabetic rats. The normal-diabetic rats exhibited the highest effect of all parameters compared to the obese-diabetic rats in the advancement of the DM period. This finding can build a platform to understand the metabolic and biochemical complications of both types of DM and can generate ideas for finding targeted drugs


Assuntos
Animais , Masculino , Ratos , Diabetes Mellitus Experimental/sangue , Hipoglicemiantes/farmacologia , Metaboloma , Metformina/farmacologia , Obesidade/sangue , Aminoácidos/sangue , Aminoácidos/urina , Glicemia/metabolismo , HDL-Colesterol/sangue , LDL-Colesterol/sangue , Diabetes Mellitus Tipo 1 , Diabetes Mellitus Tipo 2 , Dieta Hiperlipídica/efeitos adversos , Corpos Cetônicos/sangue , Ratos Sprague-Dawley
6.
J. physiol. biochem ; 74(3): 417-429, ago. 2018. graf, tab
Artigo em Inglês | IBECS | ID: ibc-178996

RESUMO

Cardiac arrest is one of the leading causes of death among adults in older age. Understanding mechanisms how organism responds to ischemia at global level is essential for the prevention and ischemic patient’s treatment. In this study, we used a global cerebral ischemia induced by four-vessel occlusion as an established animal model for ischemic stroke to investigate metabolic changes after 24 h reperfusion, when transitions occur due to the onset of delayed neuronal death. We also focused on the endogenous phenomenon known as ischemic tolerance by the pre-ischemic treatment. The experiments were carried out on blood plasma samples as easily available and metabolically reflecting the overall changes in injured organism. Our results imply that disturbed glycolysis pathway, as a consequence of ischemic injury, leads to the increased level of ketone bodies (acetone, acetoacetate and β-hydroxybutyrate) along with increased utilization of triacylglycerols in plasma of ischemic and ischemically preconditioned rats. Complementary to, a decreased level of glycolytic intermediates (lactate, pyruvate, acetate) with increased level of glucose was found in ischemic and preconditioned animals. The protective effect of ischemic preconditioning on metabolome recovery was demonstrated by significantly increased level of creatine compared to ischemic, non-preconditioned rats. We also document that acetoacetate, pyruvate, lactate, and leucine have the best discriminatory power between ischemic and control plasma. Conclusively, our results provide evidence that NMR spectra analysis can identify specific group of metabolites present in plasma with the capability for discrimination between individual groups of animals. In addition, an excellent feasibility for the statistical discrimination among ischemic, preconditioned, and control rats can be applied regardless of native or deproteinated plasma and also regardless of noesy or cpmg NMR acquisition


Assuntos
Animais , Masculino , Ratos , Isquemia Encefálica/sangue , Transtornos Cerebrovasculares/sangue , Precondicionamento Isquêmico/métodos , Corpos Cetônicos/sangue , Metaboloma , Traumatismo por Reperfusão/sangue , Ácido Acético/sangue , Biomarcadores/sangue , Glicemia/metabolismo , Isquemia Encefálica/patologia , Transtornos Cerebrovasculares/patologia , Creatina/sangue , Traumatismo por Reperfusão/patologia , Triglicerídeos/sangue
7.
An. R. Acad. Farm ; 82(n.extr): 234-259, oct. 2016. ilus, graf, tab
Artigo em Espanhol | IBECS | ID: ibc-157629

RESUMO

La contribución de la microbiota intestinal al desarrollo de diversas enfermedades, incluyendo la obesidad, se está estudiando minuciosamente. Aunque los mecanismos no están completamente definidos, las perturbaciones en la composición de la microbiota intestinal parecen estar relacionados con el sobrepeso, revelando alteraciones en los niveles de Bacteriodetes y Firmicutes en comparación con individuos delgados. La modulación de la comunidad bacteriana intestinal orientada a favorecer el crecimiento de bacterias ‘saludables’ y reducir las dañinas podría ser una eficaz herramienta terapéutica contra la obesidad. El consumo de dietas con alto contenido en grasa y azúcares afecta notablemente a la composición de la microbiota, alterando su equilibrio hacia patrones asociados a obesidad, siendo un punto de partida para un tratamiento de precisión de esta enfermedad. La interacción entre componentes de la dieta y la microbiota intestinal podría ser, en parte, responsable de sus beneficios para la salud, por lo que la administración de compuestos bioactivos podría promover el crecimiento de bacterias beneficiosas en detrimento de otras patógenas o asociadas a la obesidad. El impacto sobre el metaboloma de las intervenciones dietéticas y la administración de polifenoles se podría identificar mediante metabolómica no dirigida de las heces, permitiendo estratificar los individuos en función de la intervención dietética con el fin de aplicar tratamientos personalizados. Esta revisión pretende proporcionar una instantánea de este sistema complejo que comprende microbiota intestinal, dieta, polifenoles, metabolismo del individuo y obesidad, y cuyo conocimiento se beneficia de tecnologías avanzadas como la secuenciación de última generación y la metabolómica no dirigida (AU)


The contribution of the gut microbiota to the development of many diseases, including obesity, is being thoroughly explored. Although mechanisms are not fully understood, perturbations on gut microbiota composition seem to be related to overweight. Indeed, subjects with excessive body weight, show impairment in intestinal levels of Bacteriodetes and Firmicutes as compared to lean individuals. Therefore, modulation of gut bacterial community with approaches that could enhance the growth of ‘healthy’ bacteria and reduce harmful bacteria might be an effective therapeutic tool against obesity. Bi-directional interactions between natural compounds and the gut microbiota taking place at intestinal level, has been hypothesized to be partly responsible for the health beneficial outcomes. The consumption of high-fat high-sucrose diets strongly impacts gut microbiota composition impairing the bacterial balance towards an obesity-associated gut microbial pattern, which may be the basis for a precision management of obesity. Remarkably, the administration of bioactive compounds could counteract the disturbance of gut microbiota related to diet-induced obesity, promoting the growth of some beneficial bacteria while reducing some pathogenic and obesity- associated microbes. Biological outcomes exerted by dietary interventions and polyphenol administration on global host metabolome might be distinguished through a faecal non-targeted metabolomic analysis, where individuals might be also stratified in different groups based on the dietary intervention for personalized treatments . Therefore, this overview aimed to provide a snapshot of this complex system consisting of gut microbiota, diet and polyphenols, host metabolism and obesity taking advantage of advanced technologies namely next-generation sequencing and untargeted metabolomics (AU)


Assuntos
Humanos , Sobrepeso/metabolismo , Metabolômica/tendências , Obesidade/metabolismo , Microbioma Gastrointestinal/fisiologia , Polifenóis/metabolismo , Metaboloma/fisiologia , Ingestão de Alimentos/fisiologia
8.
Rev. iberoam. fertil. reprod. hum ; 33(3): 67-72, jul.-sept. 2016. tab
Artigo em Espanhol | IBECS | ID: ibc-156074

RESUMO

Las técnicas de reproducción asistida se han desarrollado con el objetivo de aumentar las tasas de implantación y reducir el número de embriones transferidos. El conocimiento sobre la morfología embrionaria y el desarrollo de la tecnología molecular aplicada al diagnóstico preimplantacional, han supuesto un gran avance en el rendimiento de los ciclos, mejorando los resultados clínicos y disminuyendo la tasa de aborto. El objetivo de esta revisión es describir el estado actual de las técnicas de diagnóstico embrionario. Técnicas como el time-lapse aplicada a la morfocinética nos permiten estudiar de manera continua el desarrollo embrionario, aportando un enfoque más dinámico. Tecnología molecular como la metabolómica dirigida o la proteómica, muestran nuevos elementos de evaluación sobre las primeras etapas del desarrollo hasta blastocisto. Estudios sobre el secretoma embrionario en medios de cultivo in-vitro intentan predecir patrones de desarrollo en los embriones. La búsqueda de estrategias de diagnóstico y biomarcadores asociados a calidad embrionaria, aneuploidías y potencial implantatorio son nuevos retos a los que se enfrenta la tecnología en medicina reproductiva


The assisted reproduction techniques have been developed with the aim of increasing implantation rates and reducing the number of embryos transferred. Knowledge about embryonic morphology and the development of molecular technology applied to preimplantation diagnosis has resulted in great progress in terms of cycle performance, improving clinical outcomes and reducing the rate of abortion. The aim of this review is to describe embryonic diagnostic techniques. Currently, techniques like time-lapse applied to morphokinetics allow us to continuously study embryonic development providing a more dynamic approach. Molecular techniques such as directed metabolomics or proteomics show new elements of evaluation since the early stages of development to blastocyst. Studies on the metabolism of embryos cultured in-vitro are providing data that improve embryo selection. The development of diagnostic strategies and biomarkers associated with embryonic quality, aneuploidy and implantatory potential are new challenges for the future in reproductive medicine technology


Assuntos
Humanos , Masculino , Feminino , Implantação do Embrião/fisiologia , Técnicas de Reprodução Assistida/instrumentação , Técnicas de Reprodução Assistida/normas , Técnicas de Reprodução Assistida , Metaboloma/fisiologia , Medicina Reprodutiva/classificação , Medicina Reprodutiva/métodos , Saúde Reprodutiva/normas , Pesquisas com Embriões , Proteômica/métodos , Técnicas de Reprodução Assistida/classificação , Técnicas de Reprodução Assistida/tendências , Biomarcadores/análise , Reação em Cadeia da Polimerase , Metabolômica/métodos
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