Proprioception Analysis following Anterior Cruciate Ligament Reconstruction using Stabilometry: A Prospective, Longitudinal Study / Análise de propriocepção por estabilometria após a reconstrução do ligamento cruzado anterior: Um estudo prospectivo e longitudinal

Abstract Objective Commonly used methods for measuring proprioception have resulted in conflicting reports regarding knee proprioception with anterior cruciate ligament (ACL) rupture and the influence of ACL reconstruction. Methods One hundred subjects (50 patients with radiologically and arthroscopically confirmed unilateral ACL rupture and 50 normal controls) were assessed with regards to proprioception using dynamic single-leg stance postural stabilometry. Instrumented knee ligament laxity and knee outcome scores were also measured. Of the 50 patients in the ACL group, 34 underwent reconstruction and were reassessed postoperatively. Results There was a significant proprioceptive deficiency in the ACL group compared with their contralateral knee ( p < 0.001) and to the control group ( p = 0.01). There was asignificant improvement in knee proprioception following ACL reconstruction compared to preoperative findings ( p = 0.003). There was no correlation between ligament laxity measurements and outcome scores. A significant correlation was found preoperatively between outcome scores and proprioception measurements. This correlation was not found post-operatively. Pre-operative proprioception testing had asignificant correlation (r = 0.46) with post-operative proprioception ( p = 0.006). Conclusion Patients with an ACL rupture had a proprioceptive deficit which improved following ligament reconstruction. Knee outcome scores had a better correlation with proprioception than ligament laxity. Proprioception may be a superior objective measure than ligament laxity in quantifying functional knee deficits and outcomes in patients with ACL ruptures. Level of Evidence III Therapeutic Study; Prospective Longitudinal Case-Control Study.

Resumo Objetivo Os métodos comumente usados para medir a propriocepção resultaram em relatos conflitantes sobre a propriocepção do joelho com ruptura do ligamento cruzado anterior (LCA) e a influência da reconstrução do LCA. Métodos A propriocepção de 100 indivíduos (50 pacientes com ruptura unilateral do LCA confirmada à radiologia e artroscopia e 50 controles normais) foi avaliada por estabilometria postural dinâmica em apoio unipodal. A lassidão ligamentar do joelho instrumentado e suas pontuações de desfechos também foram medidas. Dos 50 pacientes do grupo LCA, 34 foram submetidos à reconstrução e reavaliados no período pós-operatório. Resultados O grupo LCA apresentou deficiência proprioceptiva significativa em relaçãoaojoelho contralateral ( p < 0,001) e ao grupo controle ( p = 0,01). A propriocepção do joelho melhorou de maneira significativa após a reconstrução do LCA em relação aos achados pré-operatórios ( p = 0,003). Não houve correlação entre as medidas de lassidão ligamentar e as pontuações de desfechos. Além disso, observamos uma correlação significativa entre as pontuações de desfechos e as medidas de propriocepção antes da cirurgia. Essa correlação não foi detectada no período pós-operatório. O teste pré-operatório de propriocepção teve correlação significativa ( r = 0,46) com a propriocepção pós-operatória ( p = 0,006). Conclusão Os pacientes com ruptura do LCA apresentaram déficit proprioceptivo que melhorou após a reconstrução ligamentar. As pontuações de desfecho do joelho tiveram melhor correlação à propriocepção do que a lassidão ligamentar. A proprio-cepção pode ser uma medida objetiva superior à lassidão ligamentar na quantificação de déficits funcionais e desfechos do joelho em pacientes com ruptura do LCA. Nível de Evidência III Estudo Terapêutico; Estudo de Caso-Controle Longitudinal Prospectivo.

Analysis of Nerve Endings in the Superior Labrum-Biceps Complex by Fluorescence Immunohistochemistry and Confocal Laser Microscopy / Análise de terminações nervosas no complexo labrum-bíceps superior usando imunohistoquímica de fluorescência e microscopia a laser confocal

Abstract Objectives The capsuloligamentous structures of the shoulder work as static stabilizers, together with the biceps and rotator cuff muscles, increasing the contact surface of the glenoid cavity. Free nerve endings and mechanoreceptors have been identified in the shoulder; however, there are a few studies that describe the presence of these nerves in the biceps' insertion. The present study aimed to describe the morphology and distribution of nerve endings using immunofluorescence with protein gene product 9.5 (PGP 9.5) and confocal microscopy. Methods Six labrum-biceps complexes from six fresh-frozen cadavers were studied. The specimens were coronally cut and prepared using the immunofluorescence technique. In both hematoxylin and eosin (H&E) and immunofluorescence, the organization of the connective tissue with parallel collagen fibers was described. Results In the H&E study, vascular structures and some nerve structures were visualized, which were identified by the elongated presence of the nerve cell. All specimens analyzed with immunofluorescence and confocal microscopy demonstrated poor occurrence of morphotypes of sensory corpuscles and free nerve endings. We identified free nerve endings located in the labrum and in the bicipital insertion, and sparse nerve endings along the tendon. Corpuscular endings with fusiform, cuneiform, and oval aspect were identified in the tendon. Conclusion These findings support the hypothesis that the generation of pain in the superior labral tear from Anterior to posterior (SLAP) lesions derives from the more proximal part of the long biceps cord and even more from the upper labrum. Future quantitative studies with a larger number of specimens may provide more information on these sensory systems.

Resumo Objetivos As estruturas capsulo-ligamentares do ombro funcionam como estabilizadores estáticos, juntamente com os músculos do bíceps e do manguito rotador, aumentando a superfície de contato da cavidade glenoide. Terminações nervosas livres e mecanorreceptores foram identificados no ombro; no entanto, existem alguns estudos que descrevem a presença desses nervos na inserção do bíceps. Este estudo teve como objetivo descrever a morfologia e distribuição de terminações nervosas utilizando imunofluorescência com protein gene product 9.5 (PGP 9.5) e microscopia confocal. Métodos Foram estudados seis complexos labrum-bíceps de seis cadáveres congelados frescos. Os espécimes foram cortados coronalmente e preparados pelo método de imunofluorescência. Tanto em hematoxilina e eosina (H&E) quanto em imunofluorescência, foi descrita a organização do tecido conjuntivo com fibras paralelas de colágeno. Resultados No estudo de H&E, foram visualizadas estruturas vasculares e algumas estruturas nervosas, que foram identificadas pela presença alongada da célula nervosa. Todas as amostras analisadas com imunofluorescência e microscopia confocal demonstraram baixa ocorrência de morfotipos de corpúsculos sensoriais e terminações nervosas livres. Identificamos terminações nervosas livres localizadas no labrum, inserção bicipital e terminações nervosas esparsas ao longo do tendão. Terminais corpusculares com aspecto fusiforme, cuneiforme e oval foram identificados no tendão. Conclusão Esses achados corroboram a hipótese de que a geração de dor nas lesões labrais superiores de anterior a posterior (SLAP, na sigla em inglês) deriva da parte mais proximal do cabo longo do bíceps e ainda mais do labrum superior. Estudos quantitativos futuros com um número maior de espécimes podem fornecer mais informações sobre esses sistemas sensoriais.

Descripción y comparación de la línea lateral de tres especies de rayas eléctricas del género Narcine (Torpediniformes: Narcinidae) / Description and comparison of the lateral line of three species of electric rays of the genus Narcine (Torpediniformes: Narcinidae)

Resumen Se presenta la descripción de la composición y arreglo estructural del sistema de la línea lateral de tres especies de rayas eléctricas (Narcine bancroftii, N. entemedor y N. vermiculatus). Las tres especies muestran los canales supraorbital (SO), infraorbital (IO), hiomandibular (HYO) y de la línea lateral posterior (LLP), menos ramificados que en otros batoideos. Narcine entemedor difiere de sus congéneres por la gran ramificación de los canales SO e IO, los cuales convergen en la región inter-orbital; en N. vermiculatus estos canales presentan una condición similar, pero con una menor ramificación. En contraste N. bancroftii presenta los canales divergentes (dirigidos hacia afuera de la región inter-orbital). Los canales HYO están localizados en la periferia de los órganos eléctricos en estas especies; en N. bancroftii las ramificaciones se extienden hacia la región posterior, mientras que en las demás especies están reducidas (N. entemedor) o casi ausentes (N. vermiculatus). La LLP es de forma curva y está dirigida hacia el urostilo, asimismo muestra un gran número de ramificaciones, excepto en N. vermiculatus. La información sobre el arreglo y el número de poros sobre el sistema de canales de la LL en Narcine, puede ser de utilidad para la distinción taxonómica de sus especies, tal como ha sido corroborado para otros Torpediniformes.

Abstract This study describes the composition and structural arrangement of the lateral line system of three electric ray species (Narcine bancroftii, N. entemedor and N. vermiculatus). All three species showed the supraorbital (SO), infraorbital (IO), hyomandibular (HYO) and posterior lateral line (PLL) canals, less branched compared to other batoids. Narcine entemedor differs from its congeners by the extensive branching of the SO and IO canals, which converge in the inter-orbital region; in N. vermiculatus these canals present a similar condition, but are less branched. In contrast, N. bancroftii has divergent canals (directed outwards from the inter-orbital region). HYO canals are located on the periphery of the electric organs in these species; in N. bancroftii the branches extend towards the posterior region, whereas in the other species are reduced (N. entemedor) or almost absent (N. vermiculatus). The PLL is curved and projected towards the urostyle, it also shows a large number of ramifications, except in N. vermiculatus. Information about the LL canal system arrangement and pore number in Narcine can be useful for the taxonomic distinction of its species, as it has been corroborated in other Torpediniformes. Rev. Biol. Trop. 66(2): 586-592. Epub 2018 June 01.

Effects of plantar foot sensitivity manipulation on postural control of young adult and elderly / Efeitos da manipulação da sensibilidade plantar sobre o controle da postura ereta em adultos jovens e idosos

ABSTRACT Introduction: Subjects with sensorial losses present balance deficits. Although such condition is often observed among elderly, there is discussion concerning the dependence on sensorial information for body sway control in the elderly without sensorial losses. Purpose: We investigated the effects of foot sensitivity manipulation on postural control during upright standing in young adults and independent elderly (n = 19/group). Methods: Plantar sensitivity was evaluated by esthesiometry, and speed of center of pressure shift data during upright posture were evaluated for each foot using a baropodometer while the subjects were standing with eyes open or closed. The young adult group was evaluated for center of pressure in normal conditions and after plantar sensitivity disturbance, by immersing their feet in water and ice. Results: Young adults did not show alterations in their center of pressure after sensorial perturbation and presented, even under sensorial perturbation, better postural control than elderly subjects. The elderly showed lower foot sensitivity and greater center of pressure oscillation than young adults. Conclusion: Elderly subjects seem to rely more on foot sensitivity for control of body sway than young adults. In the elderly, a clinical intervention to improve foot sensitivity may help in upright posture maintenance.

RESUMO Introdução: Pessoas com perdas sensoriais apresentam déficits de equilíbrio. Embora esse quadro seja comum em idosos, ainda se discute o quanto idosos sem doenças que afetam as vias sensoriais dependem dessa informação para controlar oscilações corporais durante o controle da postura. Objetivo: Investigar os efeitos da perturbação da sensibilidade plantar sobre o controle da postura ereta em adultos jovens e idosos independentes (n = 19/grupo). Métodos: A sensibilidade plantar foi avaliada com estesiômetro e dados de velocidade e deslocamento do centro de pressão durante a postura de pé foram avaliados para cada pé com um baropodômetro, em condições de olhos abertos e fechados. O grupo de adultos jovens foi avaliado quanto ao centro de pressão nas condições normal e pós-perturbação da sensibilidade plantar, pela imersão dos pés em água e gelo. Resultados: Adultos não apresentaram alterações no centro de pressão em resposta à perturbação sensorial e tiveram, mesmo na condição de perturbação sensorial, melhor controle postural do que idosos. Idosos apresentaram menor sensibilidade plantar e maior oscilação do centro de pressão do que os adultos jovens. Conclusão: Idosos pareceram depender mais da sensibilidade plantar para manter o controle postural do que adultos jovens. Em idosos, intervenções clínicas que melhorem a sensibilidade plantar podem auxiliar na tarefa de manter a postura de pé.

Development of a new haptic perception instrument: a pilot study / Desenvolvimento de um novo instrumento de avaliação da percepção háptica: um estudo piloto

ABSTRACT Objective Hand sensory tests do not consider distinct physiological receptors, nor detect normal range variations concerning developmental or pathological changes. We developed an instrument with a set of tests with timing and scoring for assessing haptic perception, which is the interaction between sensory and motor systems, in surfaces exploration, by moving hands. Method Firstly, group meetings were set for test/manual conception and materials testing. The test/manual were submitted to 30 reviewers in 3 stages (10 reviewers on each stage). Results The Hand Haptic Perception Instrument (HHPI) evaluates hand sensorimotor performance on six domains: depression, elevation, texture, compressibility, weight (barognosis) and form perception. Each domain requires specific materials. Score ranges from 0 to 57, being 0 the worst rating. Conclusion This methodological process allowed the development of six domains and instructions to assess haptic perception. This version of HHPI is a pilot model. Further studies will determine reliability and normality ranges.

RESUMO Objetivo Testes de sensibilidade manual não consideram receptores fisiológicos distintos, tampouco variações do desenvolvimento normal ou patológico. Desenvolvemos um instrumento, com pontuação e tempo de desempenho, para avaliar percepção háptica, que é a interação sensório-motora na exploração de superfícies, pelo movimento das mãos. Método Reuniões de grupo foram estabelecidas para desenvolver os testes/ manual e testar materiais. O instrumento e seu manual foram submetidos a 30 revisores, em 3 estágios (com 10 revisores em cada estágio). Resultados O instrumento de avaliação da percepção háptica manual (IAPHM) avalia o desempenho sensório-motor da mão em seis domínios: depressão, elevação, textura, compressibilidade, peso (barognosia) e percepção de forma. Cada domínio requer materiais específicos. A pontuação vai de 0 a 57, sendo 0 a pior pontuação. Conclusão Esse método permitiu o desenvolvimento do instrumento para avaliar percepção háptica. Essa versão do IAPHM é um modelo piloto. Estudos futuros determinarão confiabilidade e variações de normalidade.

Análise dos mecanorreceptores e terminações nervosas livres da banda anterior doligamento glenoumeral inferior com imunofluorescência e microscopia confocal

A banda anterior do ligamento glenoumeral inferior(LGUI), possui uma importante função na estabilidade mecânica do ombro, sendo onsiderado o principal estabilizador estático do ombro, quando o braço encontra-se em abdução e rotação externa. Seis bandas anteriores do LGUI foram cuidadosamente dissecadas para avaliaçãoda morfologiados mecanorreceptorese terminações nervosas livres, buscando compreender a interação do sistema proprioceptivo e mecânico do ombro. Para imunihistoquimica foi utilizado um marcador tipo PGP9.5 (protein gene product)como anticorpo primário e Alexa Fluor 488 como anticorpo secundário, seguido de análise daslâminas pormicroscopia confocal de varredura a laser. Observou-se em todos os ligamentos corpúsculos de Meissnercom diâmetros queentre 30 e 65 μme comprimentos entre 80 e 400 μm,sendo estes descritos pela primeira vez no ligamento glenoumeral inferior;o corpúsculo dePacini, com diâmetro entre 40 e 80 μm e comprimento entre 100 e 180 μme terminações nervosas livres, onde as fibras apresentavam espessura entre 3 e 7 μm e comprimento entre 300 e 700 μm, além de terminações nervosas não classificadas, com formas irregulares, fusiformes e retangulares. Observamos que na banda anterior do ligamento glenoumeral inferior opredomínio de mecanorreceptores de adaptação rápida, tipo Meissner e Pacini. A densidade dos mecanorreceptores foi de aproximadamente 1,1026% ,sendo a densidadede 1,6102% quando a analise foi com profundidade entre 400 e 500 μme de 0,6018%...

The anterior band of the inferior glenohumeral ligament (IGHL) has an important role in the mechanical stability of the shoulder and is considered the main static stabilizer of the shoulder when the arm is in abduction and external rotation. Six anterior bands of IGHL were carefully dissected from the bone attachments to describe the morphology of the mechanoreceptors and free nerve endings and to elucidate the interaction between the proprioceptive system and shoulder mechanics. For immunostaining, a protein gene product 9.5 marker was used as the primary antibody and Alexa Fluor 488 was used as the secondary antibody, followed by image examination using confocal laser scanning microscopy. All the ligament samples contained Meissner corpuscles with a diameter between 30 and 65 μm and length between 80 and 400 μm. This is the first report of these corpuscles in IGHL. Furthermore, these ligaments contained Pacinian corpuscles, with a diameter between 40 and 80 μm and length between 100 and 180 μm, free nerve endings with fiber thickness between 3 and 7 μm and length between 300 and 700 μm, and unclassified nerve endings with irregular, spindle, and rectangular shapes. The mechanoreceptors were approximately 1.2% of the area of the ligament. There was a predominance of Meissner-and Pacini-type rapidly adapting mechanoreceptors.The density ofmechanoreceptorswas approximately 1.1026%, and the densitywhen the analysis was with a depthbetween 400 and 500μmwas 1.6102% and greaterdepthbetween 1500and 1850μm, was 0.6018%...

Oseopercepción: una puesta al día / Osseoperception: an update

La incorporación de implantes dentales al Sistema Estomatognático genera muchos cuestionamientos, entre ellos: ¿Cómo se explica que el paciente “sienta” a través de estos sistemas de anclaje?; ¿Por qué el paciente dice que mastica mejor que con las prótesis mucosoportadas?;¿Por qué a pesar de haber perdido dos componentes fisiológicos esenciales (dientes y periodonto) no se aprecian clínicamente importantes problemas dentro del sistema?; ¿Qué reordenamientos del sistema nervioso determinan la regulación motora luego de rehabilitado con estas técnicas? Es probable que las respuestas surjan a través del conocimiento de una nueva modalidad sensorial descriptacomo oseopercepción, la cual implica un reordenamiento de las áreas sensitivas y motoras de la corteza cerebral (neuroplasticidad).

Many questions arise from the introduction of dental implants into the stomatognathic system, for example: How can patients “feel” through these anchorage structures? Why does the patient feel that his mastication is improved with respect to the classic complete dentures? Why there are not remarkable alterations in the function of the stomatognathic system despite the loss of two essential componentsof this system? What rearrangements of the nervous system take place after the placement of dental implants that control the motor regulation of the stomatognathic system? Probably, the answer to these questions may come from the study of a new sensorymodality known as osseoperception, which involves a rearrangement of sensory and motor areas of the brain cortex (neuroplasticity).

Control of respiration in fish, amphibians and reptiles

Fish and amphibians utilise a suction/force pump to ventilate gills or lungs, with the respiratory muscles innervated by cranial nerves, while reptiles have a thoracic, aspiratory pump innervated by spinal nerves. However, fish can recruit a hypobranchial pump for active jaw occlusion during hypoxia, using feeding muscles innervated by anterior spinal nerves. This same pump is used to ventilate the air-breathing organ in air-breathing fishes. Some reptiles retain a buccal force pump for use during hypoxia or exercise. All vertebrates have respiratory rhythm generators (RRG) located in the brainstem. In cyclostomes and possibly jawed fishes, this may comprise elements of the trigeminal nucleus, though in the latter group RRG neurons have been located in the reticular formation. In air-breathing fishes and amphibians, there may be separate RRG for gill and lung ventilation. There is some evidence for multiple RRG in reptiles. Both amphibians and reptiles show episodic breathing patterns that may be centrally generated, though they do respond to changes in oxygen supply. Fish and larval amphibians have chemoreceptors sensitive to oxygen partial pressure located on the gills. Hypoxia induces increased ventilation and a reflex bradycardia and may trigger aquatic surface respiration or air-breathing, though these latter activities also respond to behavioural cues. Adult amphibians and reptiles have peripheral chemoreceptors located on the carotid arteries and central chemoreceptors sensitive to blood carbon dioxide levels. Lung perfusion may be regulated by cardiac shunting and lung ventilation stimulates lung stretch receptors.

Demanda do sistema nervoso central no controle da postura ereta humana: um modelo em malha aberta e malha fechada / Central nervous system demand on human erect posture control: an open and closed loop model

A maneira como os seres humanos controlam o equilíbrio na postura ereta não é ainda totalmente compreendida e como o sistema nervoso central controla e mantém a postura ereta quieta ainda é alvo de discussão. Nos modelos de controle postural existentes observa-se uma variação contínua do sinal do elemento final de controle, o que certamente pode comprometer a integridade do mesmo e provocar grande demanda de processamento. Visando sua preservação, uma solução seria aumentar o período de comutação, fazendo com que o elemento final de controle atue somente quando o balanço postural afastar-se consideravelmente do sinal de referência. Uma forma de implementar essa solução é empregar controladores que possuam uma zona morta ou intervalo diferencial em torno do valor de referência, definida por um limite superior e um limite inferior. O presente trabalho teve como objetivo investigar se modelos de controladores com intervalo diferencial são adequados para representar o controle da postura ereta. Para tanto, foi implementado um modelo de pêndulo simples para representar a dinâmica do sistema músculo-esquelético humano no plano sagital, com o sistema de controle neural enviando comandos para gerar um torque corretivo que resiste ao desvio da posição do corpo. Implementou-se um modelo de controle por feedback, onde o desvio da posição de referência é percebido e corrigido por um controlador PID que se assemelha a parâmetros neuromusculares, acrescido das características passivas visco-elásticas do músculo. O modelo foi simulado para limites superiores e inferiores de até 0,5º do sinal de referência, onde neste intervalo o sistema age como malha aberta. Observou-se que a opção do controlador com intervalo diferencial piora a qualidade do controle, mas solicita menos o elemento final de controle.

The necessary demand to stabilize the human posture is associated with the way Central Nervous System controls and keeps the quiet erect posture. In existing posture control models, a continuous variation of final element control signal is observed, which will certainly wear the controller. A solution to preserve it would be the increase in commutation period, causing the final control element to act only when postural balance is distantly related to reference signal. A way to implement this solution is to use controllers with a differential gap around the reference signal, defined by a superior and inferior limit. A simple pendulum model is used to represent the dynamics of the human musculo skeletal system on sagittal plane, with neural control system sending instructions in order to produce a corrective torque that stand up to body position deviation. A feedback control model was carried out, where there reference position deviation is perceived and corrected by a PID controller, that resembles neuromuscular parameters, added to passive viscous-elastic properties of the muscle. A model for superior and inferior limits up to 0.5° of reference signal was simulated. In this gap the system acted as an open loop, therefore without the correction of reference signal. It was observed that the option of controller with differential gap worsens the quality of control, but demands less the final element of control.

The influence of gender and bruxism on human minimum interdental threshold ability

OBJECTIVE: To evaluate the influence of gender and bruxism on the ability to discriminate minimum interdental threshold. MATERIAL AND METHODS: One hundred and fifteen individuals, representing both genders, bruxers and non-bruxers, with a mean age of 23.64 years, were selected for this study. For group allocation, every individual was subjected to a specific physical examination to detect bruxism (performed by three different examiners). Evaluation of the ability to discriminate minimum interdental threshold was performed using industrialized 0.010 mm-, 0.024 mm-, 0.030 mm-, 0.050 mm-, 0.080 mm- and 0.094 mm-thick aluminum foils that were placed between upper and lower premolars. Data were analyzed statistically by multiple linear regression analysis at 5% significance level. RESULTS: Neither gender nor bruxism influenced the ability to discriminate minimum interdental threshold (p>0.05). CONCLUSIONS: Gender and the presence of bruxism do not play a role in the minimum interdental threshold.

Streptozotocin-induced mechanical hypernociception is not dependent on hyperglycemia

Since streptozotocin (STZ)-induced diabetes is a widely used model of painful diabetic neuropathy, the aim of the present study was to design a rational protocol to investigate whether the development of mechanical hypernociception induced by STZ depends exclusively on hyperglycemia. Male Wistar rats (180-200 g; N = 6-7 per group) received a single intravenous injection of STZ at three different doses (10, 20, or 40 mg/kg). Only the higher dose (40 mg/kg) induced a significant increase in blood glucose levels, glucose tolerance and deficiency in weight gain. However, all STZ-treated rats (hyperglycemic or not) developed persistent (for at least 20 days) and indistinguishable bilateral mechanical hypernociception that was not prevented by daily insulin treatment (2 IU twice a day, sc). Systemic morphine (2 mg/kg) but not local (intraplantar) morphine treatment (8 µg/paw) significantly inhibited the mechanical hypernociception induced by STZ (10 or 40 mg/kg). In addition, intraplantar injection of STZ at doses that did not cause hyperglycemia (30, 100 or 300 µg/paw) induced ipsilateral mechanical hypernociception for at least 8 h that was inhibited by local and systemic morphine treatment (8 µg/paw or 2 mg/kg, respectively), but not by dexamethasone (1 mg/kg, sc). The results of this study demonstrate that systemic administration of STZ induces mechanical hypernociception that does not depend on hyperglycemia and intraplantar STZ induces mechanical sensitization of primary sensory neurons responsive to local morphine treatment.

Efeito de informação sensorial adicional na propriocepção e equilíbrio de indivíduos com lesão do LCA / Effect of additional sensory information in the proprioception and postural control of individuals with ACL lesion

OBJETIVO: Investigar o efeito da utilização de informação sensorial adicional na propriocepção e no equilíbrio de indivíduos com lesão do Ligamento Cruzado Anterior (LCA). MÉTODOS: Participaram deste estudo 28 indivíduos com lesão unilateral do LCA e 28 indivíduos com joelhos sadios. A propriocepção foi avaliada por meio do limiar para detecção de movimento passivo da articulação do joelho nas posições de 15 e 45 graus, para as direções de flexão e de extensão. O equilíbrio foi avaliado em posição unipodal sem visão, sobre uma plataforma de força e investigado por meio da amplitude e da velocidade média de oscilação do centro de pressão. As condições de informação sensorial utilizadas foram: informação normal, bandagem infra-patelar e faixa infra-patelar. RESULTADOS: Indivíduos com lesão do LCA apresentam um prejuízo na propriocepção e no equilíbrio quando comparados a indivíduos com joelhos sadios (p<0,05). Entretanto, com adição de informação sensorial, tanto a capacidade proprioceptiva quanto o equilíbrio de indivíduos com lesão do LCA é melhorado (p<0,05). Indivíduos com joelhos sadios não apresentam benefício na utilização de informação sensorial adicional (p>0,05). CONCLUSÃO: A lesão do LCA acarreta um prejuízo da propriocepção e do equilíbrio, porém estes efeitos são minimizados com utilização de informação sensorial adicional.

OBJECTIVE: The purpose of this study was to examine the effect of additional sensory information in proprioception and postural balance of individuals with ACL lesion. METHODS: Participated in this study 28 individuals with ACL unilateral lesion and 28 individuals with healthy knee. Proprioception was evaluated through the threshold to detection of passive knee motion from 15 and 45 degrees, for flexion and extension directions. Postural balance was evaluated in single leg stance without vision, on a plate force and investigated through the mean sway amplitude, mean sway velocity of the center of pressure. The conditions of sensory information used were: normal information, infrapatellar adhesive tape and infrapatellar strap. RESULTS: Individuals with ACL lesion show a deficit in proprioception and postural balance when compared with individuals with healthy knee (p<0.05). However, with the use of additional sensory information, proprioception and postural control performance in individuals with ACL lesion improve (p<0.05). Individuals with healthy knee did not show any benefit with the use of additional sensory information (p>0.05). CONCLUSION: ACL lesion causes damage in the proprioception and postural control system. However, these effects are minimized with the use of additional sensory information.

Training-related changes in the R-R interval at the onset of passive movements in humans

The aim of the present study was to determine whether training-related alterations in muscle mechanoreflex activation affect cardiac vagal withdrawal at the onset of exercise. Eighteen male volunteers divided into 9 controls (26 ± 1.9 years) and 9 racket players (25 ± 1.9 years) performed 10 s of voluntary and passive movement characterized by the wrist flexion of their dominant and non-dominant limbs. The respiratory cycle was divided into four phases and the phase 4 R-R interval was measured before and immediately following the initiation of either voluntary or passive movement. At the onset of voluntary exercise, the decrease in R-R interval was similar between dominant and non-dominant forearms in both controls (166 ± 20 vs 180 ± 34 ms, respectively; P > 0.05) and racket players (202 ± 29 vs 201 ± 31 ms, respectively; P > 0.05). Following passive movement, the non-dominant forearm of racket players elicited greater changes than the dominant forearm (129 ± 30 vs 77 ± 17 ms; P < 0.05), as well as both the dominant (54 ± 20 ms; P < 0.05) and non-dominant (59 ± 14 ms; P < 0.05) forearms of control subjects. In contrast, changes in R-R interval elicited by the racket players' dominant forearm were similar to that observed in the control group, indicating that changes in R-R interval at the onset of passive exercise were not attenuated in the dominant forearm of racket players. In summary, cardiac vagal withdrawal induced by muscle mechanoreflex stimulation is well-maintained, despite long-term exposure to training.

Extensor indicis proprius transfer for the abducted small finger

PURPOSE: Persistent abduction of the small finger has usually been treated by transfer of the extensor digiti minimi muscle. However, anatomic variations of the extensor system may limit the potential for a successful extensor digiti minimi transfer. Therefore, we evaluated the outcomes of an alternative reconstruction method for the abducted small finger using an extensor indicis proprius (EIP) transfer. METHODS: We performed 8 EIP transfers in 8 patients with persistent, flexible abduction posturing of the small finger. The primary etiology of the deformity was incomplete motor reinnervation after surgeries for ulnar neuropathy in 6 patients, rupture of the third palmar interosseous musculotendinous unit in 1 patient, and intrinsic muscle fibrosis in 1 patient. The EIP was elongated by splitting the tendinous portion and was transferred to the distal and radial part of the extensor hood. Surgical outcomes were assessed by comparing preoperative and postoperative active adduction and abduction motion of the 2 ulnar digits. RESULTS: At the mean follow-up of 23 months, the average adduction angle improved from 19 degrees to 1 degrees postoperatively. In terms of active finger motion, 6 patients showed excellent results, 1 good, and 1 fair, without loss of flexion and extension. No patient had an extension lag or complained of functional deficits of the donor index finger. There was not adverse change to digital function or range of motion for the middle and ring fingers that are crossed by the EIP. CONCLUSIONS: Extensor indicis proprius transfer can be a reliable option for correction of abduction deformity of the small finger, maintaining active abduction and full flexion and extension. TYPE OF STUDY/LEVEL OF EVIDENCE: Therapeutic IV.

Sistema sensório-motor articular: revisão de literatura / Joint sensorimotor system: a literature review

Tradicionalmente, a estabilidade articular é considerada como a perfeita congruência entre ossos durante o movimento, sendo que muitas vezes os componentes atribuídos para esta estabilidade se resumem aos ligamentos e músculos envolvidos na articulação...

Joint stability is traditionally defined as the perfect congruence among bones during movement; the components of such stability are often limited to related ligaments and muscles. Although these structures are the greatest joint stabilizers, motor-sensory integrity is fundamental for an efficient joint stability...

Mecanoreceptores da mucosa palatina de avestruz (Struthio camelus): estudo ao microscópio de luz / Mechanoreceptors of the palatine mucosa of ostrich (Struthio camelus): light microscope study

Foram estudados corpúsculos de Herbst da mucosa palatina de avestruz em nível de microscopia de luz. Os corpúsculos compõem-se de uma cápsula externa, cápsula interna e axônio central. A cápsula externa apresentou numerosas lamelas, enquanto que a cápsula interna mostrou estrutura de folhas compactas. Os corpúsculos apresentaram formato ovalado ou circular e circundado por espessos feixes de fibras colágenas. Cada lamela estava composta de uma densa rede de fibras espessas. Os axônios terminais estavam situados ao longo do eixo, terminando em um bulbo terminal. As fibras da cápsula externa, coradas por Picrosirius e examinadas no microscópio óptico sob luz polarizada, revelou a presença de fibras colágenas do tipo I em verde e na região periférica observou-se grande quantidade de fibras colágenas do tipo III. Os corpúsculos apresentaram-se envoltos por células planas e envoltos por fibras colágenas.

Herbst corpuscles of the palatine mucosa of ostrich were studied by light microscopy. The corpuscles are composed of an outer core, inner core and central nerve terminal. The outer core presents numerous lamellae, while the inner core shows compact structure of cytoplasm sheets. The corpuscles are elongate or oval in shape and are surrounded by bundles of collagen fibers. Each lamella is composed of a dense network of thick fibrils. The terminal axons are located along the axis and form a bulb terminal. The fibers of external core stained by Picrosirius and examined by polarized light microscopy revealed to be green in color like type I collagen fibers, and at the periphery is a large amount of collagen type III. The corpuscles are surrounded by flat cells and dense collagen fibers at the periphery.

Estudo comparativo dos mecanorreceptores dos discos intervertebrais normais e degenerados da coluna lombar de humanos pela radiografia, ressonância magnética e estudo anatomopatológico / Comparative study of normal and degenerated intervertebral discs' mechanoreceptors of human lumar spine X-ray, magnetic resonance and anatomopathologic study

Os autores fizeram um estudo da coluna lombar de humanos, objetivando avaliar e determinar os diferentes tipos de fibras nervosas no disco intervertebral normal e no degenerado. Foram usadas dez colunas lombares de cadáveres com aproximadamente 48 a 72 horas de óbito. As peças foram submetidas a exames de radiografia simples e ressonância magnética. Após os exames, os discos foram classificados em normais e degenerados. Em seguida, foram dissecados, divididos em regiões anterior e posterior, incluídos em parafina e realizado estudo de imuno-histoquímica com a proteína S100. Com o auxílio de um programa de computador Image-Pro Plus (media cybernetics®), as fibras nervosas tiveram seu diâmetro medidos em micrômetros e classificadas em quatro tipos de fibras. Foram encontrados quatro tipos de fibras nervosas nas diferentes regiões discais. O número e o tipo de fibras variaram de acordo com a região e grau de degeneração do disco intervertebral. Concluíram que as fibras do tipo III são mais freqüentes na região anterior; as fibras dos tipos II e IV são mais freqüentes na região posterior, e as fibras do tipo I não apresentaram diferenças entre a região anterior e a posterior; além disso, o disco degenerado tem mais fibras nervosas que o disco normal.

The authors conducted a study on human lumbar spine, aiming to assess and determine the different kinds of nervous fibers in normal and degenerated intervertebral discs. Ten cadavers lumbar spines with approximately 48 - 72 of death have been used. The pieces were submitted to simple X-ray and magnetic resonance tests. Subsequently to the tests, discs were divided into normal and degenerated. Then, they were dissected, divided into anterior and posterior according to the region, included in paraffin and an immunohistochemical study with S100 protein was performed. With the aid of Image-Pro Plus computer software (media cybernetics) ®, nervous fibers diameters were measured as micrometers and classified into four kinds of fibers. Four types of nervous fibers were found on different disc regions. The number and kind of fibers varied according to the region and degree of intervertebral disc degeneration. It was concluded that type-III fibers are more common at the anterior region; type-II and type-IV fibers are more common at the posterior region, and type-I fibers do not show any differences regarding anterior and posterior regions; in addition, a degenerated disc has a higher number of nervous fibers than a normal one.

Understanding the mechanisms of lung mechanical stress

Physical forces affect both the function and phenotype of cells in the lung. Bronchial, alveolar, and other parenchymal cells, as well as fibroblasts and macrophages, are normally subjected to a variety of passive and active mechanical forces associated with lung inflation and vascular perfusion as a result of the dynamic nature of lung function. These forces include changes in stress (force per unit area) or strain (any forced change in length in relation to the initial length) and shear stress (the stress component parallel to a given surface). The responses of cells to mechanical forces are the result of the cell's ability to sense and transduce these stimuli into intracellular signaling pathways able to communicate the information to its interior. This review will focus on the modulation of intracellular pathways by lung mechanical forces and the intercellular signaling. A better understanding of the mechanisms by which lung cells transduce physical forces into biochemical and biological signals is of key importance for identifying targets for the treatment and prevention of physical force-related disorders.

Analysis of antenal sensilla patterns of Rhodnius prolixus from Colombia and Venezuela

Antennal sensilla patterns were used to analyze population variation of domestic Rhodnius prolixus from six departments and states representing three biogeographical regions of Colombia and Venezuela. Discriminant analysis of the patterns of mechanoreceptors and of three types of chemoreceptors on the pedicel and flagellar segments showed clear differentiation between R. prolixus populations east and west of the Andean Cordillera. The distribution of thick and thin-walled trichoids on the second flagellar segment also showed correlation with latitude, but this was not seen in the patterns of other sensilla. The results of the sensilla patterns appear to be reflecting biogeographic features or population isolation rather than characters associated with different habitats and lend support to the idea that domestic R. prolixus originated in the eastern region of the Andes.