Anthropometric and dietary assessment of patients with glycogenosis type i / Avaliação antropométrica e dietética de pacientes com glicogenose tipo i

ABSTRACT Objective: To perform anthropometric and dietary evaluation of patients with glycogenosis type Ia and Ib. Methods: This cross-sectional study is composed of a sample of 11 patients with glycogenosis divided into two subgroups according to the classification of glycogenosis (type Ia=5 and type Ib=6), aged between 4 and 20 years. The analyzed anthropometric variables were weight, height, body mass index, and measures of lean and fat body mass, which were compared with reference values. For dietary assessment, a food frequency questionnaire was used to calculate energy and macronutrients intake as well as the amount of raw cornstarch consumed. Mann-Whitney U test and Fisher's exact test were performed, considering a significance level of 5%. Results: Patients ingested raw cornstarch in the amount of 0.49 to 1.34 g/kg/dose at a frequency of six times a day, which is lower than recommended (1.75-2.50 g/kg/dose, four times a day). The amount of energy intake was, on average, 50% higher than energy requirements; however, carbohydrate intake was below the adequacy percentage in 5/11 patients. Short stature was found in 4/10 patients; obesity, in 3/11; and muscle mass deficit, in 7/11. There were no statistical differences between the subgroups. Conclusions: In patients with glycogenosis type I, there was deficit in growth and muscle mass, but no differences were found between the subgroups (Ia and Ib). Although the diet did not exceed the adequacy of carbohydrates, about 1/3 of the patients presented obesity, probably due to higher energy intake.

RESUMO Objetivo: Realizar avaliação antropométrica e dietética de pacientes com glicogenose tipos Ia e Ib. Métodos: Estudo transversal composto de uma amostra de 11 pacientes com glicogenose divididos em dois subgrupos de acordo com a classificação da glicogenose (tipo Ia=5; tipo Ib=6), com idades entre 4 e 20 anos. As variáveis antropométricas analisadas foram peso, estatura, índice de massa corporal e medidas de massa magra e gorda, que foram comparadas com valores de referência. Para avaliação dietética, foi utilizado um questionário de frequência alimentar para cálculo de ingestão de energia e macronutrientes, além da quantidade de amido cru ingerida. Realizaram-se testes U de Mann-Whitney e exato de Fisher, com nível de significância de 5%. Resultados: Os pacientes ingeriram amido cru na quantidade de 0,49 a 1,34 g/kg/dose na frequência de seis vezes ao dia, inferior à dosagem preconizada (1,75-2,50 g/kg/dose quatro vezes ao dia). A quantidade de energia consumida foi, em média, 50% a mais que as necessidades, contudo o consumo de carboidratos foi abaixo da porcentagem de adequação em 5/11 pacientes. Baixa estatura ocorreu em 4/10 pacientes, obesidade em 3/11 e déficit de massa muscular em 7/11. Não houve diferença estatística entre os subgrupos. Conclusões: Em pacientes com glicogenose tipo I, houve déficit de crescimento e de massa muscular, mas não diferença significante entre os subgrupos (Ia e Ib). Embora a dieta não tenha ultrapassado a adequação de carboidratos, 1/3 dos pacientes apresentou obesidade, provavelmente pela maior ingestão de energia.

Characterising four Sarconesiopsis magellanica (Diptera: Calliphoridae) larval fat body-derived antimicrobial peptides

BACKGROUND The inappropriate use of antibiotics has led to the accelerated growth of resistance to antibiotics. The search for new therapeutic strategies (i.e., antimicrobial peptides-AMPs) has thus become a pressing need. OBJECTIVE Characterising and evaluating Sarconesiopsis magellanica larval fat body-derived AMPs. METHODS Fat body extracts were analysed by reversed-phase high-performance liquid chromatography (RP-HPLC); mass spectrometry was used for characterising the primary structure of the AMPs so found. ProtParam (Expasy) was used for analysing the AMPs' physico-chemical properties. Synthetic AMPs' antibacterial activity was evaluated. FINDINGS Four new AMPs were obtained and called sarconesin III, IV, V and VI. Sarconesin III had an α-helix structure and sarconesins IV, V and VI had linear formations. Oligomer prediction highlighted peptide-peptide interactions, suggesting that sarconesins III, V and VI could form self-aggregations when in contact with the microbial membrane. AMPs synthesised from their native molecules' sequences had potent activity against Gram-positive bacteria and, to a lesser extent, against Gram-negative and drug-resistant bacteria. Sarconesin VI was the most efficient AMP. None of the four synthetic AMPs had a cytotoxic effect. MAIN CONCLUSIONS S. magellanica larval fat body-derived antimicrobial peptides are an important source of AMPs and could be used in different antimicrobial therapies and overcoming bacterial resistance.

Fechamento de comunicação buco-sinusal utilizando o corpo adiposo bucal: Relato de Caso Clínico / Closure of oroantral communications using buccal fat pad: technique report

Introdução: As comunicações buco-sinusais (CBS) são complicações cirúrgicas relativamente frequentes na prática odontológica, relacionadas à extração dos molares superiores. As CBS permitem a passagem de alimentos e outras substâncias para o interior do seio maxilar, propiciando, assim, quadros infecciosos conhecidos como Sinusites. O tratamento das CBS é desafiador. Objetivo: Relatar um caso clínico de fístula bucossinusal tratada pela técnica de fechamento com o corpo adiposo bucal. Relato de caso: paciente do gênero masculino, 59 anos, leucoderma, sem alterações de ordem sistêmica, compareceu ao ambulatório de Pós-graduação da Universidade do Sagrado Coração relatando como queixa principal o fato de "sair líquidos pelo nariz" durante alimentação e havia iniciado após realizar exodontia de raiz residual do dente 16. O mesmo relatou que após a exodontia foi realizada uma tentativa de fechamento da fístula buco-sinusal através do reposicionamento do retalho vestibular e não foi obtido sucesso. Após 45 dias de condicionamento da mucosa do seio maxilar através de irrigações com soro fisiológico e clorexidina a 0,12% foi proposto ao paciente e realizado o fechamento da fístula buco-sinusal através da utilização do corpo adiposo bucal. Conclusão: atualmente para se alcançar o alto índice de sucesso no fechamento de comunicações busco-sinusais utilizamos esta técnica descrita, por ser um procedimento simples e que porprociona resultados satisfatórios ao paciente.

Introduction: Bucosinusal communications are relatively frequent surgical complications in dental practice. Although several conditions are related to this type of communication, the exodontia of upper posterior teeth presents as the main etiological factor. They are diagnosed through fistulas, which communicate the buccal and sinus cavities, thus allowing the passage of food and other substances into the maxillary sinus, thus providing infectious pictures known as Sinusites. Objective: through the report of a clinical case of buccosalusal fistula, we will focus on the success of the closure of buccomasinal communication with Bichat Adipose Body. Technique report: male patient, 59 years old, leucoderma, with no systemic alterations, who attended the University of the Sacred Heart Postgraduation outpatient clinic reporting as a main complaint the fact of "getting fluids through the nose" during feeding and that this had started after performing a residual root exodon of element 16. It also reported that after the exodontia, an attempt was made to close the buccosinusal fistula through the repositioning of the vestibular flap and no success was achieved. After 45 days of conditioning the maxillary sinus mucosa through irrigations with saline solution and 0.12% chlorhexidine, the patient was proposed and the buccosinusal fistula was closed using the Bichat Adipose Body. Conclusion: Today, in order to achieve the high success rate in the closure of buscossinusal communications, we use the Bichat Adipose Body, because it is a simple procedure and provides satisfactory results for the patient.

Utilização de corpo adiposo bucal em comunicação oroantral / Use of body adiposo body in oroantral communication

Introdução: A comunicação oroantral tem como principais causas etiológicas os processos patológicos, traumatismos e cirurgias, sendo comum sua ocorrência durante exodontias de elementos dentários superiores posteriores devido ao íntimo contato dos ápices radiculares com o assoalho do seio maxilar. Relato de caso: Paciente do sexo feminino, com 51 anos de idade, apresentando comunicação oroantral há 6 anos após exodontia do elemento dental 16. Realizado fechamento da comunicação oroantral sob anestesia local, com tracionamento e sutura da bola de Bichat sobre a falha óssea. Após um ano de proservação, observamos a ausência de comunicação bucosinusal bem como de sintomatologia, resultando no sucesso do tratamento. Considerações Finais: A utilização do retalho pediculado do corpo adiposo bucal é uma alternativa útil para o fechamento e tratamento de comunicações bucosinusais, sendo que o deslizamento da bola de Bichat é um método seguro para fechamento de tais comunicações e que esta é uma técnica de simples execução, com poucas complicações e limitações... (AU)

Introduction: The oroantral communication has as main etiological causes the pathological processes, traumatisms and surgeries. Its occurrence during the exodontia of posterior superior dental elements is due to the intimate contact of the root apices with the floor of the maxillary sinus. Case report: A 51-year-old female patient, presenting oroantral communication 6 years ago after the dental element 16 was extubated. The oroantral communication was closed under local anesthesia with Bichat's ball traction and suture on the bone defect. After 1 year of proservation, we observed the absence of oral communication, as well as symptomatology, resulting in the success of the treatment. Final considerations: It is concluded that the use of the pedicle flap of the buccal adipose body is a useful alternative for the closure and treatment of buco sinusal communications, and the sliding of the bichat ball is a safe method for closing such communications and that this is a technique of simple execution, with few complications and limitations... (AU)

Beetle (Ulomoides dermestoides) fat improves diabetes: effect on liver and pancreatic architecture and on PPARgamma expression

Ulomoides dermestoides is a beetle traditionally consumed to treat diabetes. In this study, we performed a composition analysis of U. dermestoides to obtain the principal fractions, which were used to assess the effect on glycemia, liver and pancreatic architecture, and PPARγ and GLUT4 expression. Normal mice and alloxan-induced diabetic mice were administered fractions of chitin, protein or fat, and the acute hypoglycemic effect was evaluated. A subacute study involving daily administration of these fractions to diabetic mice was also performed over 30 days, after which the liver and pancreas were processed by conventional histological techniques and stained with hematoxylin and eosin to evaluate morphological changes. The most active fraction, the fat fraction, was analyzed by gas chromatography-mass spectrometry (GC-MS), and PPARγ and GLUT4 mRNA expressions were determined in 3T3-L1 adipocytes. The protein and fat fractions exhibited hypoglycemic effects in the acute as well as in the 30-day study. Only the fat fraction led to elevated insulin levels and reduced glycemia, as well as lower intake of water and food. In the liver, we observed recovery of close hepatic cords in the central lobule vein following treatment with the fat fraction, while in the pancreas there was an increased density and percentage of islets and number of cells per islet, suggesting cellular regeneration. The GC-MS analysis of fat revealed three fatty acids as the major components. Finally, increased expression of PPARγ and GLUT4 was observed in 3T3-L1 adipocytes, indicating an antidiabetic effect.

Understanding fatness in the public sphere of young students: social representations and emotional response / Entendiendo la gordura en la esfera pública de estudiantes jóvenes: representaciones sociales y respuesta emocional / Entendendo a gordura no universo público dos jovens: representações sociais e resposta emocional

This study examines how youth collectively represent fatness and determines the emotions it arouses. Understanding how fatness is socially constructed by young people is crucial to create programs that better deal with it. A free association exercise elicited by the word "fatness" was answered by 200 people of the Autonomous Community of the Basque Country (Spain), and the content was analyzed by its lexicon using Alceste software. The results showed that health-related representation of fatness was mostly descriptive, and it was not connected to risky or any emotional response. But fatness was also completely represented as a social pressure issue related to stigmatization and highly correlated with negative emotions, such as sadness, insecurity, embarrassment, anguish, lonesomeness, pity or anger. That is, risky and negative emotions were linked to social non-acceptance, and not with health problems. Thus, the conclusion is that fatness is transmitted from fear and not from a positive construction of the health.

Este estudio examina de qué forma la juventud representa la gordura colectivamente y determina las emociones que suscita. Entender cómo se construye la gordura socialmente por parte de la gente joven, es crucial para crear programas que puedan lidiar mejor con ella. Para ello se llevó a cabo un ejercicio de asociación libre, suscitado por la palabra "gordura", que fue respondido por 200 personas de la Comunidad Autónoma del País Vasco (España). Su contenido fue analizado a través de su léxico, usando el software Alceste. Los resultados mostraron que la representación que relacionaba la gordura con la salud fue en su mayor parte descriptiva, y no estaba conectada con ninguna respuesta emocional o relacionada con el riesgo. Sin embargo, la gordura fue también representada como una presión social, relacionada con la estigmatización, y altamente correlacionada con emociones negativas como la tristeza, inseguridad, vergüenza, angustia, soledad, lástima o ira. Es decir, las emociones relacionadas con el riesgo y negativas se vincularon a una no aceptación social, y no a problemas de salud. Por tanto, la conclusión es que la gordura se transmite partiendo desde el temor y no desde una construcción positiva de lo que significa la salud.

O estudo examina as representações coletivas dos jovens em relação à gordura e identifica as emoções que suscita. Entender a construção social da gordura pelos jovens é crucial para poder criar programas para lidar melhor com a questão. Um exercício de associação livre evocada pela palavra "gordura" foi respondido por 200 pessoas da Comunidade Autônoma do País Basco (Espanha), e o conteúdo foi submetido a análise lexical com o uso do programa Alceste. Os resultados mostram que a representação da gordura em relação à saúde era predominantemente descritiva, sem associação com o risco ou com qualquer resposta emocional. Entretanto, a gordura também era representada enquanto questão de pressão social relacionada à estigmatização, fortemente correlacionada com emoções negativas como tristeza, insegurança, embaraço, ansiedade, solidão, pena ou raiva. Ou seja, as emoções arriscadas e negativas estiveram ligadas à não-aceitação social, e não a problemas de saúde. Portanto, a conclusão é que a representação da gordura é transmitida por medo, e não por uma construção positiva da saúde.

Correlations of condition factor and gonadosomatic, hepatosomatic and lipo-somatic relations of Leptodactylus macrosternum (ANURA: Leptodactylidae) in the Brazilian Semi-arid

ABSTRACT The objective of this study was to assess variations of the condition factor (K1) in relation to the gonadosomatic- RGS and energy reserves (hepatosomatic - RWL and liposomatic - RFB relations) of Leptodactylus macrosternum and their relationship to climate variation in the Northeast of Brazil, Caatinga area, state of Paraiba. The animals were captured fortnightly through active collecting, between January and December 2013. Significant differences were observed in the monthly variations of K1, RGS and RFB indices in male and female L. macrosternum over the months of collection. In males, K1 showed no significant relationship with the other variables. In females, RGS values only show notable correlations with RWF and K1 values. K1 values showed significant correlations with all other weight and length ratios. Climate change in the HFOB region showed significant relationships with the variation of the indexes evaluated, with the exception of RWF. The variation of K1, RGS, RWL and RFB values over the months of collection as well as their relation with the local climatic variation, showed a brief reproductive activity for the species.

Alterations in the oxidative metabolism of Rhipicephalus (Boophilus) microplus ticks in response to exposure to the insect growth regulator fluazuron / Alterações no metabolismo oxidativo de carrapatos Rhipicephalus (Boophilus) microplus em resposta à exposição ao regulador de crescimento fluazuron

Abstract Aiming to characterize the potential off-target effects of fluazuron on ticks, biochemical analyses were conducted to evaluate changes in the carbohydrate metabolism of Rhipicephalus (Boophilus) microplus ticks after exposure to fluazuron. Hemolymph and fat body were collected from female ticks before and after (4, 8 and 15 days) exposure to fluazuron. Spectrophotometric analyses were done to quantify glucose concentration and lactate dehydrogenase (LDH) activity in the hemolymph and the concentration of glycogen in the tick’s fat body. High Performance Liquid Chromatography (HPLC) was employed to determine the concentration of carboxylic acids in the hemolymph and to evaluate changes in intermediary metabolic processes requiring oxygen consumption. Increases in the levels of LDH activity and lactic acid concentration indicated that fluazuron enhanced fermentative metabolism in ticks. Exposure to fluazuron was also found to increase glucose concentrations in the hemolymph over time, although no significant differences were noted daily. In addition to expanding the body of knowledge about the mode of action of fluazuron, investigations into these mechanisms may also be useful in discovering new and as yet unexplored secondary effects.

Resumo Com o objetivo de caracterizar os efeitos não-alvo da ação do fluazuron, foram realizados testes bioquímicos para analisar possíveis alterações no metabolismo de carboidratos em carrapatos Rhipicephalus (Boophilus) microplus após sua exposição ao composto. Foram coletados hemolinfa e corpo gorduroso de fêmeas ingurgitadas antes e após (4, 8 e 15 dias) a exposição ao fluazuron. Análises espectrofotométricas foram usadas para quantificar a concentração de glicose e a atividade da lactato desidrogenase (LDH) na hemolinfa e concentração de glicogênio no corpo gorduroso. Cromatografia Líquida de Alta Eficiência (CLAE) foi usada para determinação das concentrações de ácidos carboxílicos na hemolinfa e avaliar possíveis alterações em metabolismo intermediário em relação ao consumo de oxigênio. Aumento na atividade de LDH e concentração de ácido lático indicaram que o fluazuron pode regular o metabolismo fermentativo em carrapatos. A exposição ao fluazuron também aumentou a concentração de glicose na hemolinfa, apesar de não ter havido diferença significativa na comparação entre as médias no mesmo dia de avaliação. Além de aumentar o conhecimento sobre o modo de ação do fluazuron, investigações sobre tais mecanismos também são úteis no descobrimento de novos efeitos secundários ainda não explorados.

Tratamento de fístula bucossinusal com o uso de retalho do corpo adiposo de Bichat û relato de caso / Treatment of oroantral communications using pedicled buccal fat pad graft û case report

Fístulas bucossinusais são comunicações epitelizadas entre o meio oral e o seio maxilar. Ocorrem principalmente como complicação de intervenções cirúrgicas orais e maxilofaciais. Pequenas comunicações geralmente são autorresolutivas, porém nas comunicações maiores, um tecido epitelial pode desenvolver-se em torno do seu trajeto, configurando, assim, uma fístula bucossinusal. Vários métodos de tratamento para fístula bucossinusal têm sido descritos na literatura. Devido à facilidade de acesso e rico suprimento sanguíneo, o corpo adiposo bucal é adequado para obliteração de defeitos posteriores da maxila, tanto na região de palato duro e mole, como na região alveolar e retromolar. Este trabalho objetiva relatar o caso clínico de uma fístula bucossinusal na região de rebordo alveolar maxilar esquerdo proveniente de um procedimento cirúrgico para levantamento de seio maxilar realizado há dois meses. Foi realizada antibioticoterapia para controle da infecção sinusal e tratamento cirúrgico da fístula pela técnica de retalho pediculado do corpo adiposo de Bichat, onde o mesmo foi dissecado, fracionado para a área comprometida e estabilizado à mucosa adjacente. A paciente encontra-se em acompanhamento há um ano sem queixas e/ou sinais de recidiva...

Oroantral fistula are epithelized communications between the oral environment and the maxillary sinus. They occur mainly as a complication of oral and maxillofacial surgery. Small communications are usually self resolving, but in major communications, an epithelial tissue can develop around your path, so setting a oroantral fistula. Various methods of treatment for buccal sinus fistula have been described in literature. Due to the ease of access and rich blood supply, the buccal fat pad is suitable for obliteration of later defects of the jaw, both in the area of hard and soft palate, as in alveolar and retromolar region. This is a case report of a oroantral fistula on the left maxillary alveolar region from a surgical procedure to maxillary sinus survey conducted two months ago. Antibiotic therapy was performed to control sinus infection and surgical treatment of fistula by pedicle flap technique of the fat pad of Bichat, where it was dissected, split to the affected area, and stabilized to the adjacent mucosa. The patient has been followed for a year without complaints and/or signs of recurrence...

Validez de la bioimpedancia para estimar la composición corporal de mujeres entre los 18 y 40 años / Validation of bioelectrical impedance equations for predicting body composition in women

Antecedentes: la aplicación de la bioimpedancia para estimar la composición corporal se ha incrementado recientemente en Colombia. Objetivo: evaluar la validez de la bioimpedancia para estimar el porcentaje de grasa corporal (%GC) en mujeres de Medellín-Colombia. Materiales y métodos: estudio transversal con una muestra de 52 mujeres entre los 18 y 40 años. El %GC se estimó por hidrodensitometría, bioimpedancia mano-pie con las ecuaciones de Sun y Kotler, y bioimpedancia pie-pie con báscula Tanita. Resultados: los %GC obtenidos por Kotler (31,9±6,0) y Tanita (25,6±6,1) fueron diferentes (p<0,001) del obtenido por hidrodensitometría (29,6±5,3), asimismo, estas técnicas presentaron un bajo grado de acuerdo con este método de referencia, Kotler (Bland-Altman: -2,3 IC95%: -10,9; 6,1) y Tanita (Bland-Altman: 4.0 IC95%: -6,3; 14,3). La ecuación de Sun estimó valores similares a la hidrodensitometría (29,6±5,3 vs. 28,6±5,2, p=0,098) pero presentó un bajo grado de acuerdo con este método (Bland-Altman: 0,9 IC95%: -7,1; 9,0). Conclusiones: la bioimpedancia mano-pie con la ecuación de Kotler y pie-pie con báscula Tanita, no fueron válidas para estimar el %GC de las mujeres evaluadas. La bioimpedancia mano-pie con la ecuación de Sun estimó de forma adecuada el %GC grupal, pero presentó poca validez para estimar el %GC individual en esta población.

Background: The application of bioimpedance to estimate body composition in Colombia has increased in the last years. Objective: To validate bioimpedance equations to assess fat mass percentage (%FM) in women from Medellín- Colombia. Materials and methods: This is a cross sectional study with a sample of 52 women 18 to 40 years old. The %FM was assessed by the reference method hydrodensitometry and by hand-to-feet bioimpedance with the equations of Sun and Kotler, and feet-to-feet bioimpedance with a Tanita scale. Results: The %FM estimated by Kotler (31,9±6,0) and Tanita (25,6±6,1) were different (p<0.001) from hydrodensitometry (29,6±5,3). These techniques showed low agreement with the reference method, Kotler (Bland-Altman: -2,3 IC95%: -10,9; 6,1) and Tanita (Bland- Altman: 4.0 IC95%: -6,3; 14,3). The %FM estimated by the Sun equation was similar to hydrodensitometry (29,6±5,3 Vs. 28,6±5,2, p=0,098). However, this equation showed low agreement with this method (Bland-Altman: 0,9 IC95%: -7,1; 9,0). Conclusions: The hand-to-feet bioimpedance with the Kotler equation and feet-to-feet bioimpedance with Tanita scale were not valid to assess the %FM of this specific population of women. The hand-to-feet bioimpedance with the Sun equation estimated accurately the average group of %FM, but it lacked of validity to assess the individual %FM in this population.

Cuerpo adiposo bucal, su utilización en cirugía oral / Buccal fat body, its use in oral surgery

El cuerpo adiposo bucal es de gran utilidad en el tratamiento de defectos intraorales. La técnica descrita ha demostrado ser más sencilla,mejor tolerada y con menor tasa de complicaciones, que otras técnicas descriptas anteriormente. Por estos motivos es importante considerar a este elemento anatómico al momento de cerrar comunicaciones bucosinusales. El presente trabajo presenta la utilizacióndel mismo en tres casos de comunicación buco sinusal, todas de etilogía odontógena.

Fat body bucal is very useful when treating intraoral defects. The technique has proven to be simpler, more tolerable and shows a lowercomplication rate than the utilization of other techniques previously described. This is why it's important to consider this anatomic element when closing communication between oral and nasal cavities. We present the utilization of said element in three cases of communication between cavities, all of them of odontogenic etiology.

A comparative study of fat body morphology in five mosquito species

The insect fat body plays major roles in the intermediary metabolism, in the storage and transport of haemolymph compounds and in the innate immunity. Here, the overall structure of the fat body of five species of mosquitoes (Aedes albopictus, Aedes fluviatilis, Culex quinquefasciatus, Anopheles aquasalis and Anopheles darlingi) was compared through light, scanning and transmission electron microscopy. Generally for mosquitoes, the fat body consists of lobes projecting into the haemocoel and is formed by great cell masses consisting of trophocytes and oenocytes. Trophocytes are rich in lipid droplets and protein granules. Interestingly, brown pigment granules, likely ommochromes, were found exclusively in the trophocytes located within the thorax and near the dorsal integument of Anopheles, which is suggestive of the role these cells play in detoxification via ommochrome storage. This study provides a detailed comparative analysis of the fat body in five different mosquito species and represents a significant contribution towards the understanding of the structural-functional relationships associated with this organ.

Changes in the fat body during the post-embryonic development of the predator Toxorhynchites theobaldi (Dyar & Knab) (Diptera: Culicidae)

Several studies have focused on understanding the biochemistry and morphology of the fat body of the hematophagous mosquito Aedes aegypti (L.) (Diptera: Culicidae). In contrast, few studies, if any, have focused on morphological characters of the fat body in other mosquitoes, especially non-hematophagous taxa such as the culicid Toxorhynchites. Larvae of Toxorhynchites prey upon the larvae of other mosquito species and are used in vector mosquito control. We investigated aspects of the fat body trophocytes, including the morphometric analyses of the lipid droplets, protein granules and nuclei, during Toxorhynchites theobaldi (Dyar & Knab) post-embryonic development. Following the body weight increase from larval stage L2 to L4, the size of lipid droplets within the trophocytes also increase, and are likely the result of lipogenesis. Lipid droplets decrease in size during L4 to the female pupal stage and increase once again during the period from newly-emerged to mature adult females. Protein granules are observed for the first time in female pupae, and their appearance might be related to protein storage during metamorphosis. The size of the nucleus of trophocytes also increases during larval development, followed by a decrease during metamorphosis and an additional increase as adult female ages. In conclusion, the morphology of the fat body of T. theobaldi changes according to the developmental stage. Our study provides for the first time important insights into T. theobaldi fat body development and contributes to understand this species biology.

Corpo adiposo da bochecha: um caso de variação anatômica / Cheek adiposo corpuscle: a case for anatomic variation

O corpo adiposo da bochecha é uma massa esférica de gordura, encapsulada e encontrada nabochecha. Tem uma função mecânica, servindo como coxim para facilitar a movimentação de um músculo em relação a outro nos movimentos de sucção e de mastigação. Observa-se ainda seu uso como enxerto para reconstrução de defeitos oroantrais e oronasais. Durante a dissecação de cadáveres realizada na Disciplina de Anatomia Humana do Instituto deCiências Biomédicas da Universidade Federal de Uberlândia, observou-se um corpo adiposo da bochecha de tamanhosignificativamente maior que o normal. Tal caso, no universo analisado e considerado, representa 1,2%. Sendo assim, devido a sua raridade e ao seu grande desenvolvimento volumétrico e sua proximidade com ramos do nervo facial, justifica-se a descrição deste relato de caso aos profissionais que estudam e realizam intervenções na região anatômica da face, evitando-se assim possíveis lesões do referido nervo.

The cheek fat corpuscle a spherical mass of fat encapsulated and found in cheek. It has a mechanical function, serving as a cushion to facilitate the movement of a muscle regarding the movements of anothermuscle of suction and chew. We note that its use as a graft for reconstruction of defects oroantrais and oronasais. During the dissection of corpses held in the Human Anatomy discipline of the Biomedical Sciences Institute of the Federal University of Uberlândia there was a cheek fat corpuscle in size significantly higher than normal. This case, analyzed and considered in the universe, accounts for 1.2%. Therefore, because of its rarity and its large volume development and itsproximity to the facial nerve branches, appropriate to describe this case report to the professionals who study and carry out interventions in anatomical region of the face, thus avoiding possible injury to that nerve.

Allosteric regulation of 6-phosphofructo-1-kinase activity of fat body and flight muscle from the bloodsucking bug Rhodnius prolixus

6-phosphofructo-1-kinase (phosphofructokinase; PFK) activity from Rhodnius prolixus, a haematophagous insect which is usually a poor flyer, was measured and compared in two metabolically active tissues - flight muscle and fat body. The activity of this important regulatory glycolytic enzyme was much more pronounced in muscle (15.1 ± 1.4 U/mg) than in fat body extracts (3.6±0.4 U/mg), although the latter presented higher levels of enzyme per protein content, as measured by western-blotting. Muscle extracts are more responsible than fat body to ATP and fructose 6-phosphate, both substrates of PFK. Allosteric regulation exerted by different effectors such as ADP, AMP and fructose 2,6-phosphate presented a singular pattern for each tissue. Optimal pH (8.0-8.5) and sensitivity to pH variation was very similar, and citrate was unable to inhibit PFK activity in both extracts. Our results suggest the existence of a particular PFK activity for each tissue, with regulatory patterns that are consistent with their physiological roles.

A atividade da fosfofrutocinase (PFK) de Rodnius prolixus, um inseto hematófago, o qual vôa somente pequenas distâncias, foi medida e comparada em dois tecidos metabolicamente ativos - músculo de asa e corpo gorduroso. A atividade desta importante enzima glicolítica regulatória foi muito mais pronunciada em músculo de asa (15,1 ±1,4 U/mg) do que em extrato de corpo gorduroso (3,6 ±0,4 U/mg) embora este último tenha apresentado níveis mais altos da enzima por quantidade de proteína, como medido por western-blotting. Extratos de músculo foram mais responsivos do que corpo gorduroso para ATP e frutose-6-fosfato, ambos substratos da PFK. A regulação alostérica exercida por diferentes efetores tais como ADP, AMP, frutose-2,6-bisfosfato apresentou um padrão singular para cada tecido. O pH ótimo (8,0-8,5) e a sensibilidade a variações de pH, foram muito similares e o citrato foi incapaz de inibir a atividade da PFK em ambos os extratos. Nossos resultados sugerem a existência de uma atividade particular da PFK para cada tecido com padrões regulatórios que são consistentes com suas funções fisiológicas.

Selamento de fístula buco sinusal utilizando o corpo adiposo da bochecha. Breve revisão da literatura e relato de caso clínico / Oroantral fistula closure using buccal fat pad. Brief review of literature and report of clinical case

As fístulas buco sinusais são complicações geralmente relacionadas com as exodontias dos dentes superiores posteriores, devido à proximidade existente entre os ápices desses elementos dentais e o assoalho do seio maxilar. O presente trabalho objetiva, com base em berve revisão da literatura e na experiência dos autores, mostrar as vantagens e desvantagens da utilização do corpo adiposo da bochecha para o selamento das fístulas oroantrais e apresentar um caso clínico incomum de um paciente portador de fístula buco sinusal bilateral que se submeteu a essa modalidade de tratamento.

Ultramorphology and histochemistry of fat body cells from last Instar larval of the Pachycondyla (=Neoponera) villosa (Fabricius) (Formicidae: Ponerinae)

As células do corpo gorduroso de Pachycondyla (=Neoponera) villosa distribuem-se como uma única camada entre a cutícula e o trato digestivo, formando um conjunto de células agrupadas e recobertas por uma fina membrana. Não foram identificados tipos celulares distintos por meio da ultramorfologia, porém a histologia revelou três tipos celulares distintos: os trofócitos, mais abundantes, as células de urato, distribuídas por entre os trofócitos, e os enócitos, menos abundantes que os demais. Os enócitos são comumente observados próximos da cutícula. Histoquimicamente, os trofócitos apresentaram reação positiva para proteínas básicas no núcleo e no citoplasma e reação fortemente positiva nos grânulos citoplasmáticos. O teste para carboidratos foi fortemente positivo em todo o citoplasma, enquanto para os lipídeos observou-se reação positiva nas vesículas citoplasmáticas. Em relação às células de urato, estas apresentaram reação positiva para proteínas básicas no núcleo e citoplasma, por entre as vesículas. Essas células não apresentaram reação para o teste de PAS e Sudan Black B. Quanto aos enócitos, estes apresentaram citoplasma fracamente positivo ao PAS e fortemente positivo ao Sudan Black B e para o azul de bromofenol.

Flight metabolism in Panstrongylus megistus (Hemiptera: Reduviidae): the role of carbohydrates and lipids

The metabolism of lipids and carbohydrates related to flight activity in Panstrongylus megistus was investigated. Insects were subjected to different times of flight under laboratory conditions and changes in total lipids, lipophorin density and carbohydrates were followed in the hemolymph. Lipids and glycogen were also assayed in fat body and flight muscle. In resting insects, hemolymph lipids averaged 3.4 mg/ml and significantly increased after 45 min of flight (8.8 mg/ml, P < 0.001). High-density lipophorin was the sole lipoprotein observed in resting animals. A second fraction with lower density corresponding to low-density lipophorin appeared in insects subjected to flight. Particles from both fractions showed significant differences in diacylglycerol content and size. In resting insects, carbohydrate levels averaged 0.52 mg/ml. They sharply declined more than twofold after 15 min of flight, being undetectable in hemolymph of insects flown for 45 min. Lipid and glycogen from fat body and flight muscle decreased significantly after 45 min of flight. Taken together, the results indicate that P. megistus uses carbohydrates during the initiation of the flight after which, switching fuel for flight from carbohydrates to lipids.

Fechamento de comunicaçäo bucossinusal usando enxerto pediculado com corpo adiposo bucal / Closure of oro-antral and/or oro-nal communication using a pedicled buccal fat pad graft

As fístulas bucossinusais säo ocorrências comuns e quase sempre estäo relacionadas à remoçäo dos molares superiores, principalmente porque há uma relaçäo anatômica íntima entre o ápice das raízes destes dentes com o seio maxilar. Normalmente uma fístula bucossinusal com menos de 2 mm de diâmetro fecha-se espontaneamente, mas quando este defeito é igual ou maior que 3 mm, ou o seio ou a regiäo periodontal estäo comprometidos por um processo inflamatório ou infeccioso, a abertura persiste freqüentemente. Na literatura há relatos de várias técnicas cirúrgicas para o fechamento de fístula bucossinusal, porém, estes procedimentos nem sempre apresentam resultados satisfatórios. Neste trabalho os autores avaliam o uso do enxerto pediculado com o corpo adiposo bucal para fechamento de fístula bucossinusal, e concluem que o procedimento tem larga aplicaçäo e um alto grau de sucesso

Ultrastructural features of the fat body and oenocytes of Rhinocricus padbergi Verhoeff (Diplopoda, Spirobolida)

The fat body of the diplopod Rhinocricus padbergi is located in two preferential areas of its body: a) immediately below the tegument, denominated parietal, and b) filling the body cavity, close to the viscera, mainly the ovaries and alimentary canal, denominated perivisceral. Ultrastructurally, its cells, the adipocytes, presented varied morphology and contained organelles indicating that they are cells that mainly produce and store lipids and proteins. The presence of cells similar to the oenocytes found in insects was observed for the first time in diplopods, associated to the fat body of R. padbergi. Our observations suggest that this tissue probably maintains activity cycles, since the presence of cells undergoing apoptosis was detected.