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1.
Medicentro (Villa Clara) ; 27(2)jun. 2023.
Artigo em Espanhol | LILACS | ID: biblio-1440543

RESUMO

Se presenta el caso clínico de un paciente asistido en el servicio de Dermatología por tener lesión tumoral gigante en calcáneo derecho de instauración progresiva. La biopsia incisional muestra sarcoma de Kaposi endémico sin afectación visceral. El estadio tan avanzado de la enfermedad propició la evolución tórpida del paciente. El estudio histopatológico estableció el diagnóstico certero de la lesión tumoral; la biopsia fue el método auxiliar que estableció el vínculo necesario entre el examen macroscópico y microscópico de la piel, y la interrelación básico-clínica entre dos disciplinas: Anatomía Patológica y Dermatología.


We present a clinical case of a patient seen in the Dermatology service due to a progressive giant cell tumour in the right calcaneus. Incisional biopsy shows endemic Kaposi's sarcoma without visceral involvement. The advanced stage of the disease led to the torpid evolution of the patient. The histopathological study established the accurate diagnosis of the tumour lesion, the biopsy was the auxiliary method that established the necessary link between the macroscopic and microscopic examination of the skin and the basic and clinical relationship between two disciplines: Pathological Anatomy and Dermatology.


Assuntos
Sarcoma de Kaposi , HIV , Simplexvirus , Antirretrovirais
3.
Braz. J. Pharm. Sci. (Online) ; 58: e18637, 2022. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1364416

RESUMO

Abstract The therapeutic drugs to treat Herpes simplex virus (HSV) infections have toxic side effects and there has been an emergence of drug-resistant strains. Therefore, the search for new treatments for HSV infections is mounting. In the present study, semi-solid formulations containing a crude hydroethanolic extract (CHE) from Schinus terebinthifolia were developed. Skin irritation, cutaneous permeation, and in vivo therapeutic efficacy of the formulations were investigated. Treatment with the ointment formulations did not result in any signs of skin irritation while the emulsions increased the thickness of the epidermis in Swiss mice. The cutaneous permeation test indicated that the CHE incorporated in the formulations permeated through the skin layers and was present in the epidermis and dermis even 3 h after topical application. In vivo antiviral activity in BALB/c mice treated with the CHE ointments was better than those treated with the CHE emulsions and did not significantly differ from an acyclovir-treated group. Taken together, this suggests that the incorporation of CHE in the ointment may be a potential candidate for the alternative topical treatment of herpetic lesions.


Assuntos
Preparações Farmacêuticas/análise , Simplexvirus/classificação , Herpesvirus Humano 1/classificação , Anacardiaceae/efeitos adversos , Antivirais/efeitos adversos , Aciclovir/antagonistas & inibidores , Eficácia , Emulsões/efeitos adversos
4.
Rev. odontopediatr. latinoam ; 11(1): 419130, 2021. ilus
Artigo em Espanhol | LILACS, COLNAL | ID: biblio-1417026

RESUMO

La gingivoestomatitis herpética corres-ponde a la manifestación primaria de la infección por virus herpes simple tipo I que se presenta con mayor frecuencia en lactantes mayores y preescolares. Objeti-vo: describir abordaje y manejo de gingi-vo estomatitis herpética en una paciente con cardiopatía congénita y cuadro de desnutrición severa. El caso se refiere a paciente femenina de 16 meses de edad a quien le fue realizado anamnesis, exa-men clínicoe interconsultas con servicios de pediatría, patología, medicina bucal, infectología. El diagnóstico incluyó co-municación intraventricular, desnutrición severa y gingivoestomatitis herpética. Se realizó tratamiento paliativo para el dolor, terapia antiviral (aciclovir en suspensión 1cc cada 6 horas por 7 días) y tratamiento tópico (sucralfato en suspensión 1g/5ml mezclado en partes iguales con cetirizina en suspensión. 5 mg / 5 ml, 3 veces al día directamente sobre las lesiones) durante 14 días logrando reducción de la sintoma-tologia. Conclusiones: el correcto manejo multidisciplinario permitió lograr dismi-nución en tamaño y número de las lesio-nes en cavidad oral, orientación dietética y canalización apropiada.


A gengivoestomatite herpética correspon-de à manifestação primária da infecção pelo vírus herpes simplex tipo I, que ocorre com mais frequência em bebês e em idade pré-escolar. Objetivo: descrever a aborda-gem e o tratamento da estomatite herpética gengival em um paciente com cardiopatia congênita e desnutrição grave. O caso re-fere-se a uma paciente de 16 meses de ida-de, submetida a anamnese, exame clínico e interconsultas com serviços de pediatria, patologia, medicina bucal, infectologia. O diagnóstico incluiu comunicação intraven-tricular, desnutrição grave e gengivosto-matite herpética. Foram realizados trata-mento paliativo para dor, terapia antiviral (suspensão de aciclovir 1cc a cada 6 horas por 7 dias) e tratamento tópico (suspensão de sucralfato 1g / 5ml misturado em partes iguais com suspensão de cetirizina 5mg / 5ml, 3 vezes ao dia diretamente. lesões) por 14 dias, alcançando redução dos sintomas. Conclusões: o correto manejo multidisci-plinar permitiu diminuir o tamanho e o número de lesões na cavidade oral, orien-tação alimentar e canalização adequada.


Herpetic gingivostomatitis is the pri-mary manifestation of herpes simplex virus type I infection, common in older infants and preschoolers. Objective: to describe the approach and management of herpetic stomatitis in a patient with congenital heart disease and severe mal-nutrition. The case refers to a 16-mon-th-old female patient who underwent an anamnesis, clinical examination, and inter-consultations with pediatric, pa-thological, oral medicine services, and Diagnosis included intraventricular communication, severe malnutrition, and herpetic gingivostomatitis. Palliati-ve treatment for pain, antiviral therapy (acyclovir suspension 1cc every 6 hours for 7 days) and topical treatment (sucral-fate suspension 1g / 5ml mixed in equal parts with cetirizine suspension 5mg / 5ml, 3 times a day directly, were perfor-med. about injuries) for 14 days. Conclu-sions: multidisciplinary, management, allowed to obtain, clinical diagnosis and establish a treatment plan with positive outcome,decreasing oral cavity dietary guidance and appropriate channeling.


Assuntos
Humanos , Feminino , Lactente , Antivirais , Cardiopatias Congênitas , Herpes Simples , Estomatite Herpética , Sucralfato , Aciclovir , Cetirizina , Simplexvirus , Medicina Bucal , Anamnese
5.
Arq. neuropsiquiatr ; 78(3): 163-168, Mar. 2020. tab
Artigo em Inglês | LILACS | ID: biblio-1098075

RESUMO

Abstract Herpes simplex virus (HSV) is a cause of a severe disease of the central nervous system (CNS) in humans. The demonstration of specific antibodies in the cerebrospinal fluid (CSF) may contribute to the retrospective neurological diagnosis. However, the commercial immunological tests for HSV infection are for use in serum samples. Objective: The aim of the present study was to adapt a commercial kit anti-HSV IgG used for serum samples to be performed with a CSF sample. Methods: Forty CSF specimens from 38 patients with suspected CNS HSV infection were serially diluted for detecting anti-HSV IgG by enzyme immunoassay (EIA). The same samples were also analyzed with the polymerase chain reaction (PCR). Results: The sensitivity of EIA test for HSV was 5% (dilution 1:40) and 65% (dilution 1:2) in CSF, and HSV DNA PCR was 15%. The combined analysis of EIA (dilution 1:2) and PCR increased the sensitivity up to 72.5%. The inflammatory CSF was associated with positive HSV PCR. Conclusions: We demonstrated the importance to adapt serological anti-HSV IgG EIA test for CSF assays to increase the accuracy of the analysis, considering the low concentration of specific antibodies in CSF.


Resumo O vírus herpes simples (HSV) é um dos agentes causadores de uma doença grave no sistema nervoso central (SNC) em humanos. A detecção de anticorpos específicos no líquido cefalorraquidiano (LCR) pode contribuir para o diagnóstico neurológico retrospectivo. Entretanto, os testes imunológicos comerciais são para uso em amostras de soro. Objetivo: Adaptar um kit comercial sorológico anti-HSV IgG para ser utilizado no de LCR. Metodos: Quarenta amostras de LCR de 38 pacientes com suspeita de infecção por HSV no SNC foram diluídas pesquisa de anticorpos anti-HSV IgG pelo método imunoenzimático (EIA). Além disso, as mesmas amostras também foram analisadas por reação em cadeia da polimerase (PCR). Resultados: A sensibilidade do teste EIA para o HSV consistiu em 5% (diluição 1:40) e 65% (diluição 1:2) no LCR, e o PCR do DNA do HSV, 15%. A análise combinada de EIA (diluição 1:2) e PCR aumentou a sensibilidade para 72,5%. Houve associação entre presença do LCR inflamatório e PCR positiva para HSV. Conclusões: Demonstramos a importância na adaptação previa do teste sorológico anti-HSV IgG EIA para ensaios do no LCR, a fim de aumentar a acuracia da análise, considerando a baixa concentração de anticorpos específicos no LCR.


Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Líquido Cefalorraquidiano/virologia , Simplexvirus/isolamento & purificação , Herpes Simples/diagnóstico , Herpes Simples/virologia , Anticorpos Antivirais/líquido cefalorraquidiano , Proteínas Virais , DNA Viral/genética , Reação em Cadeia da Polimerase/métodos , Estudos Retrospectivos , Simplexvirus/genética , DNA Polimerase Dirigida por DNA/genética , Exodesoxirribonucleases , Herpes Simples/líquido cefalorraquidiano , Sistema Nervoso
6.
Clinics ; 75: e1498, 2020. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1055886

RESUMO

OBJECTIVE: To evaluate the role of intraocular fluid analysis as a diagnostic aid for uveitis. METHODS: Twenty-eight samples (27 patients including 3 HIV-infected patients) with active (n=24) or non-active (n=4) uveitis were submitted to aqueous (AH; n=12) or vitreous humor (VH) analysis (n=16). All samples were analyzed by quantitative PCR for herpes simplex virus (HSV), varicella zoster virus (VZV), cytomegalovirus (CMV), Epstein-Barr virus (EBV) and Toxoplasma gondii. RESULTS: The positivity of the PCR in AH was 41.7% (5/12), with 50% (2/4) in immunocompetent and 67% (2/3) in HIV+ patients. The positivity of the PCR in VH was 31.2% (5/16), with 13% (1/8) in immunocompetent and 50% (4/8) in immunosuppressed HIV negative patients. The analysis was a determinant in the diagnostic definition in 58% of HA and 50% of VH. CONCLUSION: Even in posterior uveitis, initial AH analysis may be helpful. A careful formulation of possible clinical diagnosis seems to increase the chance of intraocular sample analysis being meaningful.


Assuntos
Humanos , Humor Aquoso/microbiologia , Humor Aquoso/parasitologia , Humor Aquoso/virologia , Uveíte/diagnóstico , Corpo Vítreo/microbiologia , Corpo Vítreo/parasitologia , Toxoplasma , Uveíte/microbiologia , Uveíte/parasitologia , Uveíte/virologia , Corpo Vítreo/virologia , DNA Viral/análise , Reação em Cadeia da Polimerase , HIV-1 , Hospedeiro Imunocomprometido , Simplexvirus/genética , Simplexvirus/imunologia , Herpesvirus Humano 4 , Herpesvirus Humano 3/genética , Herpesvirus Humano 3/imunologia , Citomegalovirus/genética , Citomegalovirus/imunologia , Imunocompetência
7.
Vaccimonitor (La Habana, Print) ; 28(3): 91-96, sept.-dic. 2019. graf
Artigo em Espanhol | LILACS, CUMED | ID: biblio-1094630

RESUMO

En los procesos neuroinflamatorios se produce a nivel de líquido cefalorraquídeo una activación policlonal y poliespecífica. Esta activación se produce desde los primeros días y puede permanecer por períodos prolongados. Luego por mecanismos de apoptosis los clones que no responden directamente contra los agentes biológicos involucrados no proliferan. El Reibergrama permite saber si las inmunoglobulinas presentes en el líquido cefalorraquídeo se sintetizaron o no en el sistema nervioso central (SNC) y el Índice de Anticuerpo (IA) determina la especificidad de las mismas en caso de que exista síntesis intratecal. Con estas herramientas nos propusimos identificar la respuesta neuroinmunológica frente a agentes de la familia herpesvirus en pacientes pediátricos con proceso inflamatorio del SNC a partir de sus respectivos IA. Para lograr esto se cuantificaron los niveles de IgG y albúmina en suero y líquido cefalorraquídeo (LCR) mediante inmunodifusión radial simple y por ensayo inmunoenzimático, con lo cual se construyó el Reibergrama que permitió la selección de 85 pacientes pediátricos con síntesis intratecal de inmunoglobulinas, que se diferenciaron en cuatro grupos según sus edades. Mediante ensayo inmunoenzimático se cuantificaron los niveles de IgG específica contra citomegalovirus, virus varicela zoster y virus herpes simple, tanto en suero como en LCR y se determinó el IA específico. La respuesta contra los virus estudiados fue similar para los distintos grupos de edades, lo cual nos permite afirmar la exposición temprana a los mismos(AU)


In a neuroinflammatory process a polyclonal and poly-specific activation is produced in cerebrospinal fluid. This activation starts from the first days and may persist for a long time. The clones not related directly against the biological agent do not proliferate by apoptosis. Reibergram determine if part of the immunoglobulins content in cerebrospinal fluid belongs from the blood or it is synthesized in the central nervous system. Antibody index determines if the specific antibodies was synthesized intrathecally. By these tools it can be possible to identify the humoral immune response against some herpes virus in pediatric patients suffering from a central nervous system inflammatory process. Quantification of specific IgG against citomegalovirus, varicella zoster and herpes simplex virus in serum and cerebrospinal fluid was done by ELISA. Specific Antibody index against these viruses were similar for the different age groups, which confirm the early exposure of the population(AU)


Assuntos
Humanos , Masculino , Feminino , Lactente , Pré-Escolar , Criança , Adolescente , Líquido Cefalorraquidiano , Simplexvirus , Infecções por Citomegalovirus/diagnóstico , Infecções por Citomegalovirus/epidemiologia , Infecção pelo Vírus da Varicela-Zoster/epidemiologia , Epidemiologia Descritiva , Estudos Transversais , Imunodifusão/métodos
13.
Rev. patol. trop ; 46(1): 01-21, abr. 2017. tab, ilus
Artigo em Inglês | LILACS | ID: biblio-913416

RESUMO

Over the past forty years, there has been a great advance in antiviral infections treatment. The discovery of acyclovir in 1977 paved the way to new antiviral drugs. Other nucleoside analogues such as valacyclovir, penciclovir, famciclovir, ganciclovir, valganciclovir, cidofovir and foscarnet were made available, as well as neuraminidase inhibitors. Also, drugs for the treatment of viral hepatitis and patients with HIV/AIDS have not only increased life quality and expectancy, but also decreased the incidence of some viral infections. Antiviral drugs are important tools to the clinician, especially when treating patients with impaired immunological and clinical condition. Aiming to restore health and prevent further adverse events, the clinician must be aware of the best antiviral drug available, its proper route of administration and dosage. The aim of this review is to present the antiviral drugs currently available, focusing on treatment of common viral infections in clinical practice. A brief description of the mechanisms of action and prescription of antiviral drugs is presented, using the data available from evidence-based medicine.


Assuntos
Antivirais , Orthomyxoviridae , Simplexvirus , Herpesvirus Humano 3 , Citomegalovirus
14.
Biomédica (Bogotá) ; 36(supl.2): 201-210, ago. 2016. graf, tab
Artigo em Espanhol | LILACS | ID: lil-794032

RESUMO

Introducción. El trasplante de precursores hematopoyéticos es una alternativa en el tratamiento de diversas condiciones en la población pediátrica. La intensidad del acondicionamiento para el trasplante predispone al desarrollo de complicaciones en los receptores. Las infecciones por el virus herpes simple 1 (HSV-1), el virus herpes simple 2 (HSV-2), el citomegalovirus (CMV) humano y el virus de Epstein-Barr (EBV) son una causa importante de morbimortalidad en estos pacientes. La reactivación de infecciones latentes puede producir descargas virales asintomáticas detectables en la saliva, lo cual ayuda a determinar el comportamiento de dichas infecciones en pacientes con trasplante y a establecer el diagnóstico temprano de la reactivación. Objetivo. Evaluar el comportamiento de la descarga viral de HSV-1, HSV-2, CMV y EBV en la saliva de pacientes hospitalizados en la Unidad de Trasplante de la Fundación HOMI - Hospital de la Misericordia, entre enero y noviembre de 2012. Materiales y métodos. Se evaluaron muestras de saliva de 17 receptores de trasplante. La presencia de ADN de HSV-1, HSV-2, CMV y EBV en las muestras de saliva se detectó mediante reacción en cadena de la polimerasa convencional. Resultados. Se detectó el ADN del HSV-2 en la saliva de cuatro pacientes, del CMV en la de cuatro y del EBV en la de nueve, lo cual se asoció con leucopenia. Cuatro de los 17 pacientes presentaron cargas simultáneas de CMV y EBV. No se detectó el ADN del HSV-1. Conclusiones: Se demostró una descarga asintomática de HSV-2, CMV y EBV asociada a leucopenia en la saliva de los pacientes.


Introduction: Hematopoietic stem cell transplantation in pediatric patients is an alternative treatment for different diseases. The conditioning regimen for transplant predisposes recipients to the development of infections. Viral infections by herpes simplex virus 1 (HSV-1), herpes simplex virus 2 (HSV-2), human cytomegalovirus (CMV), and Epstein-Barr virus (EBV), are the most common, and the leading cause of morbidity and mortality among these patients. These viruses lie dormant in various cell types and the reactivation of latent infections may lead to asymptomatic viral shedding in saliva. The detection of these viruses in secretions may contribute to understand the behavioral dynamics of these viral infections in transplanted patients, and to the early diagnosis of reactivation. Objective: To assess HSV-1, HSV-2, CMV and EBV viral shedding in the saliva of patients admitted for hematopoietic stem cell transplantation at Fundación HOMI - Hospital de la Misericordia between January and November of 2012. Materials and methods: We evaluated stimulated saliva samples of 17 hematopoietic stem cell transplantation recipients weekly. We performed DNA extraction from saliva, and we evaluated the presence of DNA for HSV-1, HSV-2, CMV, and EBV by PCR. Results: While we detected HSV-2 and CMV DNA in the saliva of four patients, EBV DNA was detected in nine patients with leukopenia. In contrast, we did not detect HSV-1 DNA in saliva. Additionally, four out of the 17 patients showed a simultaneous shedding of CMV and EBV. Conclusions: By conventional PCR, we demonstrated asymptomatic HSV-2, CMV, and EBV viral shedding in saliva, associated with leukopenia.


Assuntos
Transplante de Células-Tronco Hematopoéticas , Transplante de Medula Óssea , Citomegalovirus , Herpes Simples , Herpesviridae , Herpesvirus Humano 4 , Simplexvirus
15.
Rev. chil. infectol ; 32(1): 58-70, feb. 2015. ilus
Artigo em Espanhol | LILACS | ID: lil-742540

RESUMO

Herpes simplex viruses and humans have co-existed for tens of thousands of years. This long relationship has translated into the evolution and selection of viral determinants to evade the host immune response and reciprocally the evolution and selection of host immune components for limiting virus infection and damage. Currently there are no vaccines available to avoid infection with these viruses or therapies to cure them. Herpes simplex viruses are neurotropic and reside latently in neurons at the trigeminal and dorsal root ganglia, occasionally reactivating. Most viral recurrences are subclinical and thus, unnoticed. Here, we discuss the initial steps of infection by herpes simplex viruses and the molecular mechanisms they have developed to evade innate and adaptive immunity. A better understanding of the molecular mechanisms evolved by these viruses to evade host immunity should help us envision novel vaccine strategies and therapies that limit infection and dissemination.


Los virus herpes simplex y humanos co-existen desde decenas de miles de años. Esta prolongada relación se ha traducido en la evolución y selección de determinantes virales para evadir la respuesta inmune y recíprocamente la evolución y selección de componentes inmunes del hospedero para limitar la infección viral y el daño que producen. Actualmente no existen vacunas para evitar la infección de estos virus o terapias que la curen. Los virus herpes simplex son neurotrópicos y permanecen latentes en neuronas de ganglios trigémino y dorsales, reactivándose esporádicamente. La mayoría de las recurrencias por virus herpes simplex son sub-clínicas y por tanto pasan inadvertidas. Aquí discutimos los pasos iniciales de la infección porvirus herpes simplex y los mecanismos moleculares que estos virus han desarrollado para evadir la respuesta inmune innata y adaptativa. Una mejor comprensión de los mecanismos moleculares evolucionados por estos virus para evadir la respuesta inmune del hospedero deberían ayudarnos visualizar nuevas estrategias para desarrollar vacunas y terapias que limiten su infección y diseminación.


Assuntos
Humanos , Imunidade Adaptativa/imunologia , Herpes Simples/imunologia , Evasão da Resposta Imune , Simplexvirus/patogenicidade , Apoptose/fisiologia , Interferon Tipo I/imunologia , Simplexvirus/fisiologia , Latência Viral/fisiologia , Replicação Viral/fisiologia
16.
An. bras. dermatol ; 89(3): 448-452, May-Jun/2014. tab, graf
Artigo em Inglês | LILACS | ID: lil-711599

RESUMO

BACKGROUND: Herpes simplex virus (HSV) infection is an endemic disease and it is estimated that 6095% of the adult population are infected with symptoms that are usually self-limiting, though they can be serious, extensive and prolonged in immunocompromised individuals, highlighted by the emergence of drug-resistant strains. The study of the wild-type HSV strains based on the cytopathogenic features and its antiviral sensitivity are important in the establishment of an antivirogram for controlling the infection. OBJECTIVE: This study sought to isolate and examine the cytopathological characteristics of circulating strains of the Herpes simplex virus, from clinical specimens and their sensitivity to commercially available antiherpesvirus drugs, acyclovir, phosphonophormic acid and trifluridine. METHODS: Herpes simplex virus isolation, cytopathological features and antiviral sensitivity assays were performed in cell culture by tissue culture infectious dose or plaque forming unit assay. RESULTS: From twenty-two clinical specimens, we isolated and adapted nine strains. Overall, the cytopathic effect was detected 24 h post-infection (p.i.) and the presence of syncytia was remarkable 48 h p.i., observed after cell staining. Out of eight isolates, four developed plaques of varying sizes. All the isolates were sensitive to acyclovir, phosphonophormic and trifluridine, with the percentage of virus inhibition (%VI) ranging from 49.7-100%. CONCLUSIONS: The methodology for HSV isolation and characterization is a straightforward approach, but the drug sensitivity test, regarded as being of great practical importance, needs to be better understood. .


Assuntos
Humanos , Aciclovir/farmacologia , Antivirais/farmacologia , Foscarnet/farmacologia , Simplexvirus/efeitos dos fármacos , Simplexvirus/isolamento & purificação , Trifluridina/farmacologia , Células Cultivadas , Hematoxilina , Testes de Sensibilidade Microbiana , Fatores de Tempo , Ensaio de Placa Viral
17.
Dermatol. pediátr. latinoam. (En línea) ; 12(1): 15-20, ene.-abr. 2014. ilus
Artigo em Espanhol | LILACS | ID: lil-776147

RESUMO

Presentamos un caso de infección por Herpes virus simplex adquirido por vía transplacentaria en un neonato nacido a las 34 semanas de gestación por parto vaginal. La madre presentó un único episodio de infección genital por herpes virus en la décima semana de gestación.En el momento del nacimiento el niño presentódos ampollas y lesiones de aplasia cutis que semejaba una epidermólisis bullosa. Además, se evidenciaron múltiples anomalías congénitas del sistema nervioso central, oftalmológicas y cardíacas que sustentan la infección herpética intrauterina. El test de Tzanck y la serología para Herpes virus simplex 2 (IgG) fueron positivas. La infección intrauterina por virus herpes está asociada a alta morbilidad y mortalidad pero responde a la terapia antiviral. Este diagnóstico debe ser considerado en todo neonato con una enfermedad ampollar o piel erosionada.


We present a case of Herpes simplex virus infection acquired by transplacental route in a neonate born at 34 weeks of gestation by vaginal delivery. The mother presented a single episode of genital herpes simplex virus infection in the tenth week of pregnancy. At birth, the neonate presented two blisters and lesions of aplasia cutis resembling epidermolysis bullosa. In addition, he presented multiple congenital anomalies of the brain, eyes and heart, supporting the intrauterine infection. Tzanck test and serology for Herpes simplex virus 2(IgG) were positive. Intrauterine infection by herpes virus is associated with high morbidity and mortality, however it responds to antiviral therapy. This diagnosis should be considered in any neonate with blisters or denuded skin.


Assuntos
Humanos , Masculino , Recém-Nascido , Displasia Ectodérmica , Epidermólise Bolhosa , Herpes Genital , Herpes Simples , Simplexvirus , Simplexvirus/patogenicidade , Anormalidades Congênitas , Anormalidades da Pele
18.
Rev. Soc. Bras. Med. Trop ; 46(5): 625-628, Sept-Oct/2013. tab, graf
Artigo em Inglês | LILACS | ID: lil-691413

RESUMO

Introduction Herpes simplex virus (HSV) and varicella zoster virus (VZV) are responsible for a variety of human diseases, including central nervous system diseases. The use of polymerase chain reaction (PCR) techniques on cerebrospinal fluid samples has allowed the detection of viral DNA with high sensitivity and specificity. Methods Serial dilutions of quantified commercial controls of each virus were subjected to an in-house nested-PCR technique. Results The minimum detection limits for HSV and VZV were 5 and 10 copies/µL, respectively. Conclusions The detection limit of nested-PCR for HSV and VZV in this study was similar to the limits found in previous studies. .


Assuntos
Humanos , DNA Viral/análise , Herpes Simples/diagnóstico , Herpes Zoster/diagnóstico , /genética , Reação em Cadeia da Polimerase , Simplexvirus/genética , Limite de Detecção , Sensibilidade e Especificidade
19.
Int. j. morphol ; 31(1): 254-263, mar. 2013. ilus
Artigo em Espanhol | LILACS | ID: lil-676166

RESUMO

En los últimos años el estudio de las infecciones de transmisión sexual ha cobrado gran importancia debido principalmente al incremento de estas en parejas heterosexuales y hombres que tienen sexo con hombres. En mujeres existe mucha información de epidemiología y patogénesis de estas infecciones, sin embargo, en hombres la información es muy escasa debido a que la mayoría no presenta sintomatología. En los últimos años se ha evidenciado un creciente interés en el estudio del semen como vía de transmisión, debido principalmente a la afinidad de algunos patógenos con los espermatozoides. Dentro de los principales microorganismos infectantes en semen se encuentran Chlamydia trachomatis, Neisseria gonorrhoeae, Mollicutes, Virus de la Inmunodeficiencia Humana tipos 1 y 2, Virus Herpes Simplex 1 y 2, Virus Papiloma Humano, Virus de la Hepatitis B y C, Citomegalovirus, Virus Epstein-Barr y Trichomonas vaginalis.


Sexually transmitted infections study has become an important issue in these days, mainly due to the increment of heterosexual and men have sex with men partners of people. In women, there is a lot information about epidemiology and pathogenesis of these infections. However, the information is very limited in men, because most infected men are asymptomatic. In recent years, there has been an increasing interest in study of semen as a transmission way, due to the affinity of some pathogens to sperm. The most prevalent microorganisms infecting semen are: Chlamydia trachomatis, Neisseria gonorrhoeae, Mollicutes, Human Immunodeficiency Virus Types 1 and 2 Herpes Simplex Virus 1 and 2, Human Papillomavirus, Hepatitis B and C virus, Cytomegalovirus, Epstein-Barr and Trichomonas vaginalis.


Assuntos
Humanos , Masculino , Feminino , Sêmen/microbiologia , Espermatozoides/parasitologia , Infecções Sexualmente Transmissíveis/transmissão , Sêmen/parasitologia , Espermatozoides/microbiologia , Bactérias/patogenicidade , Trichomonas vaginalis , Vírus/patogenicidade , Infecções Sexualmente Transmissíveis/microbiologia , Infecções Sexualmente Transmissíveis/parasitologia , Chlamydia trachomatis , Vírus da Hepatite B , HIV , Simplexvirus , Herpesvirus Humano 1 , Citomegalovirus , Vetores de Doenças , Neisseria gonorrhoeae
20.
Med. U.P.B ; 31(2): 181-192, jul.-dic. 2012.
Artigo em Espanhol | LILACS, COLNAL | ID: lil-689086

RESUMO

Objetivo: describir la frecuencia, y el comportamiento clínico y de laboratorio de las infecciones por citomegalovirus (CMV), virus Epstein-Barr (EBV) y virus herpes simplex 1 y 2 (HSV 1 y HSV 2) en el sistema nervioso central tanto en pacientes inmunocomprometidos como inmunocompetentes. Metodología: se analizaron 204 líquidos cefalorraquídeos con el uso de la reacción en cadena de la polimerasa (PCR, de su sigla en inglés) en tiempo real como herramienta diagnóstica molecular para la detección de infección. Resultados: un 16% de los líquidos cefalorraquídeos es positivo para alguno de los virus estudiados, el EBV se detectó en el 22.8% de los casos positivos y se ubica por encima de las infecciones ocasionadas por HSV y CMV. El 61.9% de los pacientes con resultado positivo tenía algún tipo de inmunocompromiso. onclusiones: la utilización de la PCR en tiempo real permite establecer el agente causal de infección en el sistema nervioso central y es de gran ayuda en los pacientes inmunocomprometidos en los que las variaciones clínicas y de laboratorio no son concluyentes. Esto permitiría la implementación de una terapia específica.


Objective: To describe the frequency and the clinical and laboratory characteristics of infections with cytomegalovirus (CMV), Epstein-Barr (EBV) and herpes simplex virus 1 and 2 (HSV 1 and HSV 2) in central nervous system in both immunocompetent and immunocompromised patients. Methods: A total of 204 cerebrospinal fluid samples were tested for CMV, EBV or HSV 1 and 2 using real time PCR as a molecular diagnostic tool for detection of infection. Results: Sixteen percent of cerebrospinal fluid was positive for at least one of the viruses, and Epstein Barr virus was detected in 22.8% of cases, which is above the infections caused by HSV and CMV. 61.9% of positive patients had some form of immune compromise. Conclusions: The use of real-time PCR identifies the causative agent of infection in the central nervous system and is of great help in immunocompromised patients where clinical and laboratory variations are inconclusive; this will also help to implement a specific therapy.


Objetivo: descrever a frequência, e o comportamento clínico e de laboratório das infecções por citomegalovírus (CMV), vírus Epstein-Barr (EBV) e vírus herpes simplex 1 e 2 (HSV 1 e HSV 2) no sistema nervoso central tanto em pacientes imuno-comprometidos como imuno-competentes. Metodologia: analisaram-se 204 líquidos cefalorraquídianos utilizando a reação em corrente da polimerase (PCR, de sua sigla em virilhas) em tempo real como ferramenta diagnostica molecular para a detecção de infecção. Resultados: um 16% dos líquidos cefalorraquídianos foram positivos para algum dos vírus estudados, o EBV se detectou no 22.8% dos casos positivos localizando-se acima das infecções ocasionadas por HSV e CMV. O 61.9% dos pacientes com resultado positivo tinha algum tipo de imuno-compromisso. Conclusões: a utilização da PCR em tempo real permite estabelecer o agente causal de infecção no sistema nervoso central sendo de grande ajuda nos pacientes inmunocomprometidos onde as variações clínicas e de laboratório não são concludentes e isto permitiria a implementação de uma terapia específica.


Assuntos
Humanos , Citomegalovirus , Encefalite Viral , Infecções por Vírus Epstein-Barr , Líquido Cefalorraquidiano , Meningite Asséptica , Reação em Cadeia da Polimerase , Simplexvirus
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