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1.
Braz. arch. biol. technol ; 62: e19180365, 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1055401

RESUMO

Abstract In this study, we aim to determine the shelf life of dried and ready to use-powdered soup samples obtained from different types of fish species such as Rainbow trout (Oncorhynchus mykiss), sea bream (Sparusaurata L.) and sea bass (Dicentrarchuc labrax). For this purpose, the chemical (moisture, protein, fat, carbohydrate, ash, pH, Thiobarbituric acid (TBA), Total volatile basic nitrogen (TVB-N), microbiological (total mesophilic aerobic bacteria, total yeast and mold, total coliform, Staphylococcus spp. and Salmonella spp.) and sensory properties of fish soup samples were determined during 6-month storage period. The pH, TBA and TVB-N values of all samples increased during the storage period, but these values were within the consumption limits. Additionally, the microbiological properties of all fish soup samples were found within the consumption limits during storage. As a result, a product has been obtained with high nutritional value and rich with regard to protein, oil, and minerals by adding the fish meat to soup samples. It has been determined that the product quality and shelf life significantly increased by using the boiled product for the production of soup samples.


Assuntos
Tiobarbitúricos/análise , Sopas , Armazenamento de Alimentos/normas , Peixes , Nitrogênio/análise
2.
Acta cir. bras ; 32(3): 211-218, Mar. 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-837686

RESUMO

Abstract Purpose: To evaluate the effects of hypertonic saline solution associated to remote ischemic perconditioning in renal ischemia/reperfusion injury in rats. Methods: Twenty five male rats (Wistar) underwent right nephrectomy and were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of renal ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during kidney ischemia; Hypertonic saline solution group (HSS) treated with hypertonic saline solution (4ml/kg); remote ischemic perconditioning + Hypertonic saline solution group (Per+HSS) with both treatments. After reperfusion, blood samples were collected for BUN and creatinine serum levels analyzes. TBARS were evaluated in plasma and renal tissue to assess oxidative stress. Kidney histopathological examination were performed. Results: Per+HSS group showed a lower degree of renal dysfunction in relation to I/R group, whereas the technique of remote ischemic perconditioning isolated or associated with saline solution significantly reduced oxidative stress and histological damage. Conclusion: Remote ischemic perconditioning associated or not to saline solution promoted reduction of acute renal injury induced by ischemia/reperfusion.


Assuntos
Animais , Masculino , Solução Salina Hipertônica/farmacologia , Traumatismo por Reperfusão/prevenção & controle , Precondicionamento Isquêmico/métodos , Substâncias Protetoras/farmacologia , Isquemia/prevenção & controle , Rim/irrigação sanguínea , Tiobarbitúricos/análise , Fatores de Tempo , Nitrogênio da Ureia Sanguínea , Distribuição Aleatória , Reprodutibilidade dos Testes , Resultado do Tratamento , Ratos Wistar , Estresse Oxidativo , Creatinina/sangue , Rim/fisiopatologia , Rim/patologia , Rim/química , Testes de Função Renal , Necrose
3.
Rev. bras. cir. cardiovasc ; 30(2): 173-181, Mar-Apr/2015. tab, graf
Artigo em Inglês | LILACS | ID: lil-748938

RESUMO

Abstract Introduction: Pharmacological therapy is a strategy for the prevention of complications associated with ischemia and reperfusion injury that occurs after volume replacement in the treatment of hemorrhagic shock. Objective: The aim of this study was to evaluate the effect of N-acetylcysteine associated with fluid resuscitation in cardiac injury in a rat hemorrhagic shock model. Methods: Mice Wister male rats were randomly and subjected to controlled hemorrhagic shock for 60 min. and then, subjected to resuscitation with Ringer lactate. In a group of six animals, 150mg/kg of N-acetylcysteine were added to fluid volume replacement. The animals were observed for 120 min and after this period, were euthanized and cardiac tissue was collected for histopathological analysis and measurement of thiobarbituric acid reactive substances and pro-and anti-inflammatory interleukin. Results: Cardiac tissue of the group treated with N-acetylcysteine showed lower concentrations of thiobarbituric acid reactive substances (0.20±0.05 vs. 0.27±0.05, P=0.014) and reduced histopathological damage and edema when compared to the group whose volume replacement occurred only with Ringer lactate. There was no difference in the expression of cytokines interleukin 6 (2,138.29±316.89 vs. 1,870.16±303.68, P=0.091) and interleukin 10 (1.019,83±262,50 vs. 848.60±106.5, P=0.169) between the treated groups. Conclusion: The association of N-acetylcysteine on volume replacement attenuates oxidative stress in the heart, as well myocardial damage and edema, but does not modify the expression of inflammatory cytokines. .


Resumo Introdução: A terapia farmacológica é uma estratégia de prevenção das complicações associadas à lesão de isquemia e reperfusão tecidual que ocorre após a reposição volêmica no tratamento do choque hemorrágico. Objetivo: O objetivo deste estudo foi avaliar a repercussão da N-acetilcisteína associada à reposição volêmica na lesão cardíaca em modelo de choque hemorrágico em ratos. Métodos: Ratos Wistar, machos, foram randomizados e submetidos ao choque hemorrágico controlado por 60 minutos e, depois, submetidos à reposição volêmica com Ringer Lactato. Em um grupo de seis animais, foram adicionados 150 mg/Kg de N-acetilcisteína ao fluido de reposição volêmica. Os animais foram observados por 120 minutos e após este período foram submetidos à eutanásia e coleta do tecido cardíaco para análise histopatológica e dosagem de substâncias reativas ao ácido tiobarbitúrico e interleucinas pró e anti-inflamatórias. Resultados: Foi observada menor concentração de substâncias reativas ao ácido tiobarbitúrico (0,20±0,05 vs. 0,27±0,05, P=0,014) e menor dano histopatológico e edema no tecido cardíaco do grupo tratado com N-acetilcisteína em relação ao grupo cuja reposição volêmica ocorreu somente com Ringer Lactato. Não foi observada diferença da expressão das citocinas interleucina 6 (2.138,29±316,89 vs. 1.870,16±303,68, P=0,091) e interleucina 10 (1.019,83±262,50 vs. 848,60±106,5, P=0,169) entre os grupos tratados. Conclusão: A associação da N-acetilcisteína na reposição volêmica atenua o estresse oxidativo no coração, assim como dano e edema miocárdicos, porém, não modifica a expressão de citocinas inflamatórias. .


Assuntos
Animais , Masculino , Acetilcisteína/farmacologia , Sequestradores de Radicais Livres/farmacologia , Coração/efeitos dos fármacos , Choque Hemorrágico/tratamento farmacológico , Pressão Arterial , Acetilcisteína/uso terapêutico , Hidratação/métodos , Sequestradores de Radicais Livres/uso terapêutico , /análise , /análise , Soluções Isotônicas/farmacologia , Soluções Isotônicas/uso terapêutico , Ácido Láctico/sangue , Miocárdio/metabolismo , Miocárdio/patologia , Estresse Oxidativo/efeitos dos fármacos , Potássio/sangue , Distribuição Aleatória , Ratos Wistar , Reprodutibilidade dos Testes , Traumatismo por Reperfusão/prevenção & controle , Ressuscitação/métodos , Choque Hemorrágico/metabolismo , Fatores de Tempo , Tiobarbitúricos/análise
4.
Biol. Res ; 48: 1-9, 2015. graf, tab
Artigo em Inglês | LILACS | ID: biblio-950817

RESUMO

BACKGROUND: Metabolic syndrome is a growing worldwide health problem. We evaluated the effects of wine grape powder (WGP), rich in antioxidants and fiber, in a rat model of metabolic syndrome induced by a high fructose diet. We tested whether WGP supplementation may prevent glucose intolerance and decrease oxidative stress in rats fed with a high fructose diet. METHODS: Male Sprague-Dawley rats weighing 180 g were divided into four groups according to their feeding protocols. Rats were fed with control diet (C), control plus 20 % WGP (C + WGP), 50 % high fructose (HF) or 50 % fructose plus 20 % WGP (HF + WGP) for 16 weeks. Blood glucose, insulin and triglycerides, weight, and arterial blood pressure were measured. Homeostasis model assessment (HOMA) index was calculated using insulin and glucose values. A glucose tolerance test was performed 2 days before the end of the experiment. As an index of oxidative stress, thio-barbituric acid reactive substances (TBARS) level was measured in plasma and kidney, and superoxide dismutase was measured in the kidney. RESULTS: Thiobarbituric acid reactive substances in plasma and renal tissue were significantly higher when compared to the control group. In addition, the area under the curve of the glucose tolerance test was higher in HF fed animals. Furthermore, fasting blood glucose, plasma insulin levels, and the HOMA index, were also increased. WGP supplementation prevented these alterations in rats fed with the HF diet. We did not find any significant difference in body weight or systolic blood pressure in any of the groups. CONCLUSIONS: Our results show that WGP supplementation prevented hyperglycemia, insulin resistance and reduced oxidative stress in rats fed with HF diet. We propose that WGP may be used as a supplement in human food as well.


Assuntos
Animais , Masculino , Ratos , Vinho , Intolerância à Glucose/prevenção & controle , Estresse Oxidativo/efeitos dos fármacos , Vitis/química , Síndrome Metabólica/prevenção & controle , Hiperglicemia/prevenção & controle , Fitoterapia/métodos , Pós/uso terapêutico , Superóxido Dismutase/análise , Tiobarbitúricos/análise , Triglicerídeos/análise , Glicemia/análise , Resistência à Insulina , Ratos Sprague-Dawley , Síndrome Metabólica/induzido quimicamente , Modelos Animais de Doenças , Pressão Arterial , Frutose/administração & dosagem , Teste de Tolerância a Glucose , Insulina/sangue , Rim/metabolismo , Antioxidantes/farmacologia
5.
Biol. Res ; 47: 1-7, 2014. ilus, graf, tab
Artigo em Inglês | LILACS | ID: lil-710931

RESUMO

BACKGROUND: Schistosomiasis is caused by helminth parasites of the genus Schistosoma. Berberine chloride (BER), an isoquinoline alkaloid, has been used in vivo for its antiparasitic, antioxidant and hepatoprotective properties. In this study, the protective effect of BER and praziquantel has been compared for the extent of schistosomiasis-induced oxidative stress in hepatic tissue of mice. RESULTS: S. mansoni was able to induce inflammation and injury to the liver, evidenced (i) by an increase in inflammatory cellular infiltrations, dilated sinusoids and vacuolated hepatocytes, (ii) by decreased levels of alanine and aspartate aminotransferases and increased levels of alkaline phosphatase, γ-glutamyl transferase in the liver homogenate, (iii) by increased production of nitric oxide and thiobarbituric acid reactive substances, and (iv) by lowered glutathione levels and decreased activities of catalase and superoxide dismutase, respectively. All these infection-induced parameters were significantly altered during BER treatment. In particular, berberine counteracted the S. mansoni-induced loss of glutathione and the activities of catalase and superoxide dismutase. CONCLUSION: Based on these results, it is concluded that berberine could ameliorate pre-existing liver damage and oxidative stress conditions due to schistosomiasis.


Assuntos
Animais , Camundongos , Berberina/uso terapêutico , Hepatopatias Parasitárias/tratamento farmacológico , Fígado/lesões , Estresse Oxidativo/efeitos dos fármacos , Esquistossomose/tratamento farmacológico , Alanina Transaminase/análise , Fosfatase Alcalina/análise , Aspartato Aminotransferases/análise , Catalase/metabolismo , Glutationa/análise , Infiltração de Neutrófilos , Óxido Nítrico/análise , Schistosoma mansoni , Superóxido Dismutase/metabolismo , Tiobarbitúricos/análise , gama-Glutamiltransferase/análise
6.
Acta cir. bras ; 27(8): 544-551, Aug. 2012. ilus, tab
Artigo em Inglês | LILACS | ID: lil-643622

RESUMO

PURPOSE: To evaluate the influence of aerobic exercise on oxidative stress in mice. METHODS: The study included twenty female mice Mus musculus-Swiss divided into two groups: sedentary control (GA) and exercise (GB), each containing ten animals. All animals underwent an adaptation period of seven days isolated in individual boxes. After this period, the animals in the exercise group (GB) were trained in angled running wheel with circumference of 25 cm assembled on an articulated axle during five minutes for three consecutive days. On the fourth day, they underwent an exercise program of one session lasting 45 minutes. The evaluation of oxidative stress was performed by determining the levels of malondialhyde derived of lipid peroxidation by the TBA method. The samples were read in a spectrophotometer at 535 nm. RESULTS: No significant difference was observed in the intergroup comparison of MDA levels in the tissues evaluated. A significant difference was observed in the intragroup comparison of MDA levels in the control group (p = 0.0201).The Tukeys' post hoc test indicated significantly lower values of MDA in the smooth muscle in relation to plasma. In the analysis of variance in the exercise group, a significant difference between tissues (p = 0.0009), with significantly lower values in the smooth muscle in relation to plasma (p<0.001) and higher in striated muscle in relation to smooth muscle (p<0.05) was observed. CONCLUSION: There was no change in the analysis of oxidative stress in mice which were undergone a single session of aerobic exercise.


OBJETIVO: Avaliar a influência do exercício físico aeróbico sobre o estresse oxidativo em camundongos. MÉTODOS: Participaram do estudo 20 camundongos (Swiss), distribuídos em dois grupos: controle-sedentário (GA) e exercício (GB) cada um contendo dez animais. Todos os animais passaram por um período de adaptação de sete dias. Após os animais do grupo (GB) receberam treinamento em roda giratória angulada montada sobre eixo articulado por cinco minutos durante três dias consecutivos. No quarto dia foram submetidos à única sessão de exercício por 45 minutos. A avaliação do estresse oxidativo foi realizada por meio dos níveis de malondiadeído pelo método do TBA. As amostras foram lidas em espectrofotômetro a 535nm. RESULTADOS: Não houve diferença significativa na comparação intergrupos nos tecidos avaliados. Diferença significativa foi observada na comparação intragrupo para o GA (p=0,0201). O post hoc test de Tukey apontou valores significantemente inferiores no músculo liso em relação ao plasma. A análise de variância do GB apontou diferença significativa entre os tecidos (p=0,0009), com valores menores no músculo liso em relação ao plasma (p<0,001), e maiores no músculo estriado em relação ao músculo liso (p<0,05). CONCLUSÃO: Não houve alteração nas análises de malondialdeído tecidual entre os grupos avaliados.


Assuntos
Animais , Feminino , Camundongos , Estresse Oxidativo/fisiologia , Condicionamento Físico Animal/fisiologia , Biomarcadores/análise , Peroxidação de Lipídeos/fisiologia , Malondialdeído/análise , Músculo Esquelético/química , Esforço Físico/fisiologia , Fatores de Tempo , Tiobarbitúricos
7.
Rev. colomb. cienc. pecu ; 21(3): 330-339, Sep. 2008. tab, graf
Artigo em Espanhol | LILACS | ID: lil-559410

RESUMO

Las alteraciones fisicoquímicas y sensoriales fueron evaluadas en filetes sajados de híbrido de cachama (Piaractus brachypomus x Colossoma macropomum), biopreservados con un extracto crudo de bacteriocinas producidas por Lactobacillus plantarum LPBM10, empacados al vacío y almacenados a 3 ºC/30 días. La evaluación serealizó bajo tres tratamientos, extracto crudo de bacteriocinas, ácido láctico y control.Los valores de pH disminuyeron a lo largo del periodo de almacenamiento alcanzand1 o valores de 6.20 para el final del periodo. El valor inicial del acido tiobarbitúrico (TBA) para los filetes fue de 0.93 mg de malonaldehido/kg, hacia el día 10 de almacenamiento se obtuvieron los valores más altos de TBA. Para este periodo el mayor valor fue alcanzado para el tratamiento con ácido láctico y el menor para el tratamiento control.El análisis de bases volátiles totales de nitrógeno (BVT-N) mostró los mejores resultados para el tratamiento con extracto crudo de bacteriocinas y finalizó con 19.3 mg BVT8N/100g. Los resultados del análisis sensorial para filetes sajados en estado fresco y cocinado, presentaron las mejores puntuaciones de aceptabilidad para el tratamiento con extracto crudo de bacteriocinas.


Biopreservatives for a native strain bacteriocin producer were evaluated on fillets of hybrid Cachama. A raw extract of Lactobacillus plantarum LPBM10-bacteriocin was added to fillets of cachama hybrids (Piaractus brachypomus x Colossoma macropomum)°vaccum-packed and stored at 30 °C/30 days. The evaluation was performed with three treatments, crude bacteriocin extract, lactic acid and control. The pH values decrease throughout storage period levels 6.20 at the end of the period. The initial value of thiobarbituric acid (TBA) for the fillets was 0.93 mg of malonaldehyde/kg, the highest values in physicochemical and sensory changes were evaluated to cachama hybrid fillets Piaractus brachypomus x Colossoma macropomum biopreserved with a crude bacteriocin extract produced by Lactobacillus plantarum LPBM10, vacuum-packed and stored at 30 ºC/30 days. The evaluation of TBA was obtained up the day 10 of storage. For this period the lactic acid treatment reached the highest value and the lower were observed for the control treatment. The analysis of TVB-N showed the best results for bacteriocin treatment and concluded with 19.3 mg TVB-N/100g. The results of sensory analysis for fresh and cooked cut fillets showed the best acceptability scores for the crude bacteriocin extract treatment.


As perturbações físicas e sensoriais foram avaliadas em filetes Sajid de híbridos de cachama (Piaractus brachypomus x Colossoma macropomum), biopreservados com um extrato bruto de bacteriocinas produzidas por Lactobacillus plantarum LPBM10, vácuo-embaladas e armazenadas em 3 ºC/30 dias. A avaliação foi conduzida sob três tratamentos, extrato bruto de bacteriocinas, ácido láctico e de controlo. O pH diminuiu ao longo do período de armazenagem atingindo valores de 6.20 no final do período. O valor inicial no ácido thiobarbiturico (TBA) foi para os filetes de estresse oxidativo 0.93 mg/kg a 10 dias de armazenamento foram os maiores valores de TBA. Para este período, o valor mais elevado foi atingido por tratamento com ácido láctico e os mais baixos para o tratamento controle. A análise de aminas voláteis de nitrogênio (N-BVT) apresentou os melhores resultados para o tratamento com extrato bruto de bacteriocinas e terminou com 19.3 mg BVT-N/100g. Os resultados da análise sensorial para filetes Sajid como frescos e cozidos, teve a melhor pontuação para a aceitação do tratamento com extrato bruto de bacteriocinas.


Assuntos
Animais , Bacteriocinas , Lactobacillus , Tiobarbitúricos
8.
Biol. Res ; 39(2): 281-288, 2006. ilus, tab
Artigo em Inglês | LILACS | ID: lil-432430

RESUMO

Free radicals induce numerous diseases by lipid peroxidation, protein peroxidation, and DNA damage. It has been reported that numerous plant extracts have antioxidant activities to scavenge free radicals. Whether Polygonum aviculare L. (Polygonaceae) has antioxidant activity is unknown. In this study, dried Polygonum aviculare L. was extracted by ethanol, and the extract was lyophilized. The antioxidant activities of extract powder were examined by free radical scavenging assays, superoxide radical scavenging assays, lipid peroxidation assays and hydroxyl radical-induced DNA strand scission assays. The results show that the IC50 value of Polygonum aviculare L. extract is 50 µg/ml in free radical scavenging assays, 0.8 µg/ml in superoxide radical scavenging assays, and 15 µg/ml in lipid peroxidation assays, respectively. Furthermore, Polygonum aviculare L. extract has DNA protective effect in hydroxyl radical-induced DNA strand scission assays. The total phenolics and flavonoid content of extract is 677.4 ± 62.7 µg/g and 112.7 ± 13 µg/g. The results indicate that Polygonum aviculare L. extract clearly has antioxidant effects.


Assuntos
Animais , Masculino , Camundongos , Antioxidantes/farmacologia , Dano ao DNA/efeitos dos fármacos , Sequestradores de Radicais Livres/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Polygonum/química , Flavonoides/análise , Hidroxibenzoatos , Camundongos Endogâmicos BALB C , Nitroazul de Tetrazólio/farmacologia , Picratos/farmacologia , Extratos Vegetais/farmacologia , Tiobarbitúricos/farmacologia
9.
Acta cir. bras ; 18(4): 283-288, jul.-ago. 2003. ilus, tab
Artigo em Inglês | LILACS | ID: lil-347140

RESUMO

PURPOSE: Investigate if alpha-tocopherol has a protective effect on intestinal mucosa after obstruction and to evaluate the potential relations between lipid peroxidation and bacterial translocation. METHODS: Ten rats were submitted to a sham laparotomy and six served as control group. A small bowel obstruction was done in sixteen animals and among them eight were pretreated with alpha-tocopherol. Forty-eight hours later, mesenteric lymph node, spleen, liver and blood cultures and also samples from ileal mucosal were obtained, Thiobarbituric acid reactive substances (TBARS) levels were determined and intestinal histological assessment was performed. RESULTS: Bacterial translocation was significantly increased in the obstructed rats compared with the control, sham and antioxidant pretreated groups (p< 0,05). TBARS (nmol/100mg) in untreated obstructed rats increased from 49,0 ± 13,3 in control group to 128,8 ± 40 after 48 hours of intestinal obstruction and achieved 72,3 ± 24,6 in alpha-tocopherol group (p< 0,05). Bacterial adherence to the intestinal epithelial cells surface and mucosal necrosis were significantly increased in the obstructed compared with nonobstructed rats. CONCLUSION: Alpha-tocopherol reduce the deleterious effects of the TBARS over the intestinal mucosal suggesting that in such circumstances there might be an association between bacterial translocation and lipid peroxidation after an intestinal occlusion.


Assuntos
Animais , Masculino , Ratos , Translocação Bacteriana , Radicais Livres , Obstrução Intestinal , Peroxidação de Lipídeos , Vitamina E , Tiobarbitúricos/sangue
10.
Arch. latinoam. nutr ; 35(2): 337-46, jun. 1985. tab
Artigo em Espanhol | LILACS | ID: lil-27384

RESUMO

En Venezuela es necesario aprovechar al máximo las especies sub-utilizadas de pescado que se capturan con el camarón. Este trabajo tuvo por propósito evaluar la estabilidad de tres especies de pescados sub-utilizados: bagre, cunaro y caballa, con base en los cambios que pueden ocurrir en las grasas. Ello se hizo comparando tanto la parte comestible del pescado sin deshuesar, como la carne deshuesada de cada uno de ellos. La evaluación de los cambios se efectuó por medio de los índices de acidez y peróxido, con extración previa de la grasa y el índice del ácido tiobarbitúrico (TBA). Por otra parte, se determinó el perfil de ácidos grasos de las tres especies investigadas, valiéndose de cromatografía de gases. En todos los casos, la mayor alteración se detectó en la muestra deshuesada, obteniéndose los valores más altos, tanto de TBA como del índice de acidez en el tercer mes de almacenamiento en todas las especies; luego se notó una disminución de estos valores. Por otro parte, el índice de peróxido acusó valores heterogéneos en las tres especies analizadas a lo largo del almacenamiento. Los ácidos grasos predominantes fueron: el palmítico (16:0) entre los saturados, y el oleico (18:1) entre los insaturados. En las tres especies se constató una mayor proporción de ácidos grasos insaturados. También se observó un incremento de éstos en la muestra dehuesada con respecto al pescado sin deshuesar


Assuntos
Ácidos Graxos/análise , Peixes , Tecnologia de Alimentos , Tiobarbitúricos/análise , Ácidos Oleicos/análise , Ácidos Palmíticos/análise
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